ERYTHROPOIESIS
Pronormoblast Basophilic Normoblast Polychromatophilic Normoblast Orthochromatic Normoblast Reticulocyte Erythrocyte
(Rubriblast) (Prorubriblast) (Rubricyte) (Metarubricyte)
Earliest recognizable and largest Hemoglobin synthesis begins at this Increased production of The last nucleated stage Slightly larger than the mature RBC A biconcave 6 – 8 um disc
cell of the erythrocyte series stage hemoglobin pigmentation and Cannot synthesize DNA and with residual amts of RNA Life span: 120 days
Size: 12 – 20 um Size: 10 – 15 um decreasing amounts of RNA cannot undergo cellular division Reticulocyte count: an index of Main function: to transport hemoglobin,
Nucleus: large round, oval, dark Nucleus: large round to sl oval; Last stage in which the cell is The NRBC sometimes seen in bone marrow activity or effective a protein that delivers oxygen from the
violet condensed, coarse chromatin; 0 – 1 capable of mitoses the peripheral circulation erythropoiesis lungs to tissues and cells
fine chromatin; 1 – 2 nucleoli nucleoli Size: 10 - 15 um Size: 8 - 10 um Size: 8 - 10 um Contains 90% hemoglobin and 10% H2O
Cytoplasm: deep blue spotty, Cytoplasm: deeply basophilic; Nucleus is condensed and intensely Nucleus: small pyknotic nucleus; Nucleus: anucleate cell containing normal conc of RBCs varies w/ age, sex &
basophilic w/ a perinuclear halo clusters of free ribosomes basophilic with coarse dense small amt of basophilic reticulum geographic distribution
N/C Ratio: 8:1 N/C Ratio: 6:1 heterochromatin granules. chromatin; 0 nucleoli (RNA) Size: 7 - 8 um
BM (%): 1 BM (%): 1-4 Lighter, grayish cytoplasm Cytoplasm: abundant red- Nucleoli: 0 Nucleus: anucleated cell
Round nucleus and thin rim of nuclear condensation (“fractured” Nucleoli: 0 orange cytoplasm uniform in Cytoplasm: large amt of blue-pink Nucleoli: 0
blue cytoplasm nucleus) with a deep blue color staining hemoglobin cytoplasm Cytoplasm: pink staining, zone of central
cytoplasm N/C Ratio: 1:2 pallor is 1/3 of cell diameter devoid of
No granules/Almost no nucleoli BM (%): 5-10 hemoglobin
present N/C Ratio: NA
LEUKOPOIESIS
Myeloblast Promyelocyte Myelocyte Metamyelocyte Band
first stage of the granulocytic series that is second largest stage in the granulocytic Size: 10 – 18 um Size: 10 – 16 um Size: 10 – 15 um
identifiable by light microscopy series Nucleus: central to eccentric; large, Nucleus: kidney or bean, indented, blue- Nucleus: C or S-shape; dark purple; dense
Size: 10 – 20 um Size: 12 – 21 um round,oval; blue; coarse chromatin; rarely purple; coarse and compact chromatin;no and clumpy chromatin; no nucleoli
Nucleus: large round to oval, indented on one Nucleus: large round to oval, dark blue; visible nucleoli nucleoli Cytoplasm: abundant; pink to violet; (+)
side; dark blue; 1 – 5 nucleoli often flattened; 2-3 nucleoli Cytoplasm: moderate; bluish pink or light Cytoplasm: abundant; pink; (+) specific large amount of secondary granules
Cytoplasm: scant, basophilic w/ distinct Cytoplasm: increasing in amount; medium pink, becoming light; (+) specific granules or granules or secondary granules N/C Ratio: 1:3
parachromatin blue; (+) nonspecific granules or primary secondary granules (violet) - LESS N/C Ratio: 4:1 BM (%): 10-40
N/C Ratio: 5:1 granules (red) - MORE N/C Ratio: 4:1 BM (%): 15-30
BM (%): 1-2 o N/C Ratio: 5:1 BM (%): 10-20
o BM (%): 2-5
Neutrophil Eosinophil Basophil
Size: 10 – 15 um Size: 10 – 15 um Size: 10 – 15 um
Nucleus: segmented or lobulated (2-5 lobes); dark purple; coarse and Nucleus: segmented or lobed with 1-3 lobes; dark purple and obscured by Nucleus: segmented or lobed with 1-3 lobes; dark purple; coarse and
clumped chromatin granules; coarse and clumped chromatin clumped chromatin
Cytoplasm: abundant; pink; abundant secondary granules Cytoplasm: abundant; pink; (+) large orange granules (eosinophilic) Cytoplasm: abundant; pale blue; (+) black-blue granules
N/C Ratio: 1:3 N/C Ratio: 1:3 N/C Ratio: 1:3
BM (%): 10-30 BM (%): 0-3 BM (%): 0-1
MONOPOIESIS
Monoblast Promonocyte Monocyte
Size: 12 – 20 um Size: 14 – 18 um Size: 12 – 20 um
Nucleus: round to oval with slight indentation centrally located; pale blue to Nucleus: large indentation, folding more distinct, centrally located; pale Nucleus: increased folding, often horseshoe; blue-purple; moderately
red purple; fine and lacy chromatin; 0 – 3 nucleoli red purple; slightly clumped chromatin; 0 – 2 nucleoli clumped chromatin; (-) nucleoli
Cytoplasm: moderate amount with regular border; basophilic & Cytoplasm: moderate amount with pseudopod at border; gray-blue Cytoplasm: abundant, equally proportioned around nucleus; pale gray-blue;
homogeneous; (-) granules opaque; with or w/o fine red dustlike particles (+) fine red dust-like particles & vacuoles
N/C Ratio: 4-6:1 N/C Ratio: 4-3:1 N/C Ratio: 4-2:1
BM (%): 0-3 BM (%): 0-3 BM (%): 0-3
LYMPHOPOIESIS
Lymphoblast Prolymphocyte Lymphocyte
- Size: 10 – 20 um - Size: 9 – 18 um - Size: 7 – 10 um
- Nucleus: round or oval with definite nuclear membrane; - Nucleus: ovoid, round or slightly indented; - Nucleus: round to oval with slight indentation;
blue-purple; chromatin not as fine as myeloblast; 0 – 3 nucleoli blue-purple to red-purple; clumped and coarse blue-purple; coarse and clumpy chromatin;
- Cytoplasm: scant; moderately basophilic, chromatin; o – 1 less distinct nucleoli (-) nucleoli
lighter near nucleus and darker at periphery; - Cytoplasm: moderate; clear basophilic; (-) - Cytoplasm: scant; moderate to dark blue;
(-)granules granules granulation usually absent, occasional azurophilic granules
- N/C Ratio: 4:1 - N/C Ratio: 4:1 - N/C Ratio: 4:1
- BM (%): 0-2 - BM (%): 0-1 - BM (%): 5-15
MEGAKARYOPOIESIS
Megakaryoblast Promegakaryocyte Granular Megakaryocyte Mature Megakaryocyte Platelet
