Taking Blood Cultures
 Always wash hands before taking blood cultures - hand decontamination required even if wearing gloves
 If blood being drawn for other investigations, take blood culture first before other samples
 Only take blood cultures if the patient is for active treatment with antibiotics
Step 1                                                  Step 2                                  Step3
 Collect equipment from blood culture kit in a          Clean appropriate vein                 Remove caps from
  clean tray.                                             site with ChloraPrep                    blood culture
 Make sure all items to be used are within expiry        Frepp (2% Chlorhexdine                  bottles and clean
  date.                                                   Gluconate in 70%                        bottle tops with a
                                                          Isopropyl alcohol)                      2% Chlorhexidine
                                                          applicator for 30 seconds.              in 70% alcohol
                                                         Leave to dry for 30                     wipe.
                                                          seconds.                               Leave for 30
                                                         Do not re-palpate vein.                 seconds to dry.
Step 4                                                                                          Step 5
 Gain venous access with the winged blood collection                                            Remove and dispose of sharps and clinical
  set. You may secure device with surgical tape                                                   waste.
  (micropore).                                                                                   Complete blood culture label and put into
 Standing blood culture bottle upright insert into adapter                                       patient’s notes.
  cap to gain sample (8-10 ml).                                                                  Label blood culture bottles and fully
 Fill blue/silver (aerobic) bottle first and then red/purple                                     complete specimen form.
  (anaerobic) bottle.                                                                            Ensure the bar code on the blood culture
                                                                                                  bottle is not covered by the patient label.
Heart of England Foundation Trust Infection Control Team December 2017 - VERSION 3