May 2018
Agdex 251/15-1
              Development of Greenhouse
              Lettuce Growing Techniques
                       in Alberta
L ettuce (Lactuca sativa) has excellent potential as
  a year-round greenhouse crop. Lettuce, a good
source of Vitamin A, E and folacin, is considered a
healthy food choice.
Background
Lettuce is one of the most commonly grown
greenhouse vegetables in Alberta, after cucumbers,
tomatoes and peppers, and the primary product of
greenhouse lettuce is the head or leaf.
According to Statistics Canada, the Canadian
greenhouse production of lettuce in 2014 was
19.9 hectares. Alberta consumed 21,357 tonnes of          Figure 1. Greenhouse lettuce growing systems
lettuce in 2011, about 20 to 30 per cent of Canadian
production, yet only 5 per cent of that total was         in styrofoam boards that float in a pool of nutrient
produced in Alberta.                                      solution.
Alberta greenhouse producers could increase lettuce       For greenhouse producers, re-circulating aeroponic
production and replace imports. Profitable greenhouse     and hydroponic systems allow for a very effective use
production depends on growers using production            of growing space and nutrients, and these systems are
practices that maximize productivity and quality while    well suited to the greenhouse production of high value
minimizing costs.                                         crops such as lettuce.
Lettuce growing systems                                   What is aeroponic?
Around the world, different styles of growing systems     Lettuce can be grown aeroponically in A-frames with
are used for greenhouse lettuce (Figure 1).               a nutrient solution sprayed on the bare roots (Figure
In Alberta, some large commercial greenhouses use a       2). Aeroponics is a system where the plant roots are
raft system for producing lettuce. The lettuce is grown   suspended in the air and saturated with a fine mist
of nutrient solution at designated time intervals.            Using aeroponics, the plants are totally suspended in
This technology is designed to minimize water                 air and can access up to 100 per cent of the available
consumption.                                                  oxygen in the air (Figure 3).
Figure 2. Roots suspended in air in an aeroponic system       Figure 3. Plant roots are open to the air
With aeroponics, growers can produce lettuce year             Some key elements to note:
round, with the potential for more plants per area as         •   growing method promotes root metabolism and
well as faster growth and bigger lettuce heads while              plant growth
using less water and nutrient supply.                         •   plants grow longer and faster: accelerates biomass
                                                                  growth and reduces rooting time
The development of high performance, automated                •   use of fine droplets, 20 to 100 microns, produces
aeroponic systems for lettuce production signifies an             excessive root hairs
economic advantage for Alberta-based greenhouse               •   large droplets, over 100 microns, leads to less
production for several reasons:                                   oxygen being taken up by the plants
•   improved water use efficiency (low requirement of         •   misting time: 1 to 2 minutes at different interval
    water to operate against conventional hydroponic              times up to 3 minutes
    methods)                                                  •   root formation, number of roots, root length all
•   more growing cycles per year to reduce costs                  increase as dissolved oxygen increases
    associated with greenhouse production (for                •   root zone temperature: cool nutrient solution
    example, labour)                                              holds more dissolved oxygen (DO) for root uptake
•   improved integration of automation, re-circulation            – nutrient solution carries dissolved oxygen
    and space utilization in plant culture                        greater than 7 ppm (parts per million), but nutrient
                                                                  solution temperature must be below 20 °C
These strengths may provide a cost-effective                  •   lack of oxygen (O2) can cause root cell death and
sustainable production system for greenhouse lettuce.             increase the risk of root disease such as pythium
Potential of aeroponics technique                             Root oxygenation and role of oxygen
Aeroponics is a research tool for nutrient uptake,            Good aeration of the re-circulating nutrient solution is
monitoring of plant health and optimization of                essential to provide oxygen to plant roots and reduce
crops grown in a closed environment. An aeroponic             both the incidence and severity of root rot diseases.
computerized control system will monitor key                  When oxygen levels are low in the root zone, the roots
elements within the crop management targets during            do not take up the nutrients required for growth. The
the growing season:                                           more oxygen present (greater than 7 ppm), the better
                                                              the nutrient uptake and the better the root system.
• watering spray intervals (time between spray
    applications)                                             However, root zone temperature is very important.
• spray duration (length of time for each spray               If the root zone temperature is high, then the oxygen
    application)                                              level will go down. The movement caused by the flow
• control strategies (nutrient injection and feed
    recipes)
                                                          2
of water in the aeroponic system is usually enough to
keep the oxygen level high in the solution.
Aeroponics system design
An A-frame shaped aeroponic system of one movable
bench can be built with mounted, fixed spray nozzles
connected with 2 cm (3/4 in.) diameter PVC plastic pipe
(see Figure 2). In total, the growing area would be 9 m2,
with the supporting A-frame reaching 121 cm at the
peak.
In an aeroponic production system, the plants are
suspended in an enclosed environment by misting
the plant roots with an atomized, nutrient-rich water
                                                                  Figure 4. Hydroponic system to grow plants
solution using the nozzles (each nozzle puts out 6.8
litres (1.8 gallons) of solution per hour) while the rest
of the plant (canopy) sits above the setup.                       The demand for hydroponically grown products is
                                                                  increasing fast around the world (Figure 5).
A nutrient-injection program can consist of two
stock tanks plus one acid tank. A high pressure pump
delivers nutrients to the roots with a flow rate of
1,363 litres (360 gallons) of solution per hour. Fertilizer
and irrigation water are delivered through a fully
automated aeroponic nutrient-injection system under
the control of a computer system in two nutrient
regimes.
Advantages of an aeroponics system
• water use efficiency (80 to 90% less water use in
   aeroponics versus hydroponics)
• re-use of nutrient solution
• space: plants stack vertically, roof-topping
   greenhouses
• less weight                                                     Figure 5. Demand for hydroponically grown produce is
• increase water uptake                                           increasing
• excellent aeration
Disadvantages of an aeroponics system                             Hydroponics system design
• clogged foggers, pump failure or breakdowns                     In hydroponic floating raft technology, one movable
   (pipe) going unnoticed may damage plants in a                  bench can be built with a total of 9 m2 growing area.
   short time                                                     The nutrient-rich solution can be delivered to the
• regular maintenance schedule, filter needs to be                plants by filling the benches, draining them and
   kept clean                                                     then pumping the solution back to the same bench
                                                                  continuously.
                                                                  Advantages of a hydroponic system
What is hydroponic?                                               • can grow anywhere and produce crop year-round
Hydroponics is the method growing of plants using                 • planting out and automated harvesting is easier
water and the essential nutrients required without the               than with aeroponic system
presence of soil (Figure 4).
                                                              3
Disadvantages of a hydroponic system                            The feed target (ppm) of the nutrient solution should
• if a root disease appears, all plants in the system           be maintained at the following levels:
   will be affected, and plant death will occur rapidly         •   nitrate nitrogen 165 ppm
• uses more space and water compared with                       •   phosphorus 50 ppm
   aeroponic system                                             •   potassium 210 ppm
                                                                •   calcium 180 to 200 ppm
                                                                •   iron 3 to 5 ppm
                                                                •   manganese 0.5 ppm
Growing conditions                                              •   zinc 0.1ppm
Temperature and humidity controls are also important            •   boron 0.5 ppm
for successful lettuce production.                              •   sodium molybdate 0.05 ppm
Temperature: Temperature is an important factor at
all stages of lettuce growth. Lettuce is a cool weather
crop, so long days and hot summer temperatures can              Cultivars
cause the crop to “bolt” or go to flower.                       Butterhead lettuce
                                                                1. Seed company - RIJK ZWAAN
During crop growth and production, cooling fans,                    • Alexandria:
ventilation and curtain shade cloths are used to                        ◦   has good size and performance
maintain a daytime temperature of 16 to 18 °C and                       ◦   popular cultivar in commercial greenhouse
a nighttime temperature ranging from 11 to 12 °C,                           lettuce in Alberta
depending on growth stage.
                                                                2. Seed company - BAYER
Sudden changes in temperature can favour disease                   • Frank:
development by causing condensation on leaves or
increasing the incidence of tip burn. Lettuce is highly                 ◦   great shaped lettuce with a well filled head
sensitive to freezing, which damages the leaves.                            and a good balance of outer leaves
Humidity: Excessive humidity will reduce transpiration,         3. Seed company - ENZA ZADEN
resulting in decreased nutrient transport. A relative              • Fairly:
humidity (RH) of 70 to 80 per cent is generally targeted                ◦   has remarkable reliable performance under
during lettuce production.                                                  different conditions
                                                                        ◦   high tolerance to internal tip burn
                                                                Oak leaf lettuce
Nutrient solution management                                    1. Seed company - ENZA ZADEN
Nutrient solution management allows for accurate                   • Pagero:
control of the fertilizer feed solution, which has a                    ◦   has highly attractive, nice fresh green
significant effect in increasing productivity and lettuce                   colour
crop quality.                                                           ◦   big-sized cultivar has very regular heads
Two factors have a significant effect on the growth of          Romaine lettuce
greenhouse lettuce: concentration of nutrient salts             1. Seed company - BAYER
and pH, so these elements need to be tested and                    • Intred:
monitored regularly. In the winter, lettuce should                      ◦   unique internal red colour
receive a concentration of nutrient salts EC (Electrical                ◦   very nice heart shape, medium size, good
Conductivity) from 1.9 to 2.2 mS/cm and in the                              shelf life
summer, 1.6 to 1.8 mS/cm. A pH of 5.8 for the nutrient
solution is optimum.                                            2. Seed company - BAYER
                                                                   • Thumper:
Fertilizer and acid are added to the feed tank to
maintain pH and the appropriate nutrient levels for                     ◦   compact little lettuce with a good uniform
each cultivar and stage of crop development.                                head shape
                                                                        ◦   very stable
                                                            4
Physiological disorders                                                 newly developing cells, so tip burn symptoms begin to
                                                                        appear. These symptoms will appear even when plenty
                                                                        of water-soluble calcium is in the nutrient solution.
Tip burn
Tip burn happens in both areoponic and hydroponic                       Methods to prevent this physiological disorder:
systems and with cultivars under supplemental winter
                                                                        •	 Calcium levels in the nutrient solution must be
lighting (Figure 6).
                                                                           high enough for sufficient calcium uptake by roots,
                                                                           and transpiration rates must be high enough to
                                                                           enable sufficient translocation of calcium ions to
                                                                           the growing tips.
                                                                        •	 Increasing ventilation and air circulation with fans
                                                                           will increase transpiration and help keep moisture
                                                                           from accumulating around the leaf surfaces.
                                                                        •	 Use low temperature and low light with the
                                                                           formation of the lettuce head.
                                                                        •	 Reducing the application of nitrogen, harvesting
                                                                           the lettuce slightly before maturity and
                                                                           maintaining proper humidity will help prevent tip
                                                                           burn.
Figure 6. Tip burn on greenhouse lettuce plant
                                                                        Cost effectiveness and
                                                                        productivity
Tip burn of young, inner lettuce leaves is caused                       The initial cost of building an aeroponics system
by a calcium (Ca) deficiency and is characterized by                    is higher than the initial cost required to build a
browning of the edges and tips of the leaves. Calcium                   hydroponics system.
is an immobile element and must be taken up in the
transpiration stream.                                                   Aeroponics: butterhead lettuce produces 360 heads/
                                                                        cycle at 12 cycles per year, which will produce 4,320
Environmental conditions that reduce the transpiration                  heads per year.
rate – such as sudden temperature changes, too high
relative humidity (RH) too low light or low temperature                 Hydroponics: butterhead lettuce in the same
– can result in tip burn.                                               conditions will provide 1,980 heads per year.
Under high RH, the plants are unable to evaporate                       There are significant differences between the
enough water to enable the transport of minerals                        automated aeroponic and hydroponic systems
(like Ca) to growing plant cells. In this situation,                    (Figure 7).
calcium uptake becomes insufficient to reach the
                                        Aeroponic System                               Hydroponic System
    Nutrient Solution              Nutrients in mist of water droplets             Nutrients in H2O solution
    Water Use Efficiency           Low re-circulating mist of droplets             High re-circulating flow of water
    Yield                          360 lettuce heads in 9 square metres            180 lettuce heads in 9 square metres
    Risk Factor                    High                                            Medium
   Figure 7. Differences between aeroponic and hydroponic systems
                                                                    5
Depending on the cultivar, time of the year and
supplemental winter lighting, the yield/productivity of
the two systems will be quite different (Figure 8).
                                   Yield (Number of Heads Harvested per Cycle per 9 m²)
                             400
                                                 360
                             350
   Number of Lettice Heads
                             300
                             250
                             200                                               180
                             150
                             100
                             50
                              0
                                           Aeroponic System              Hydroponic System
Figure 8. Lettuce yield/productivity differs between areoponic
and hydroponic systems
Conclusion
Alberta’s greenhouse industry is positioned for growth
through access to new technology. An increasing
number of leafy crops like lettuce are being grown
hydroponically in more and more places around the
world. Aeroponics offers another way to increase
greenhouse lettuce production in Alberta.
Prepared by
Nabeel Mohammed
Alberta Agriculture and Forestry
Greenhouse Section
Crop Diversification Centre South
Brooks, Alberta
More information
Alberta Ag-Info Centre
Call toll free 310-FARM (3276)
Website: agriculture.alberta.ca
The development of this factsheet was supported
in part by Growing Forward 2, a federal-provincial-
territorial initiative.