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QFR 1.

This document discusses several tests that can detect Rheumatic Factor (RF) besides latex agglutination. The haemagglutination test detects RF by mixing blood with sheep red blood cells coated with rabbit antibodies, causing agglutination if RF is present. Nephelometry uses light scattering to detect the formation of immune complexes between RF and aggregated human IgG, becoming more cloudy as complex numbers grow. Other past streptococcal infection tests have also been developed, such as the anti-CHO test which may help detect rheumatic fever.

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33 views2 pages

QFR 1.

This document discusses several tests that can detect Rheumatic Factor (RF) besides latex agglutination. The haemagglutination test detects RF by mixing blood with sheep red blood cells coated with rabbit antibodies, causing agglutination if RF is present. Nephelometry uses light scattering to detect the formation of immune complexes between RF and aggregated human IgG, becoming more cloudy as complex numbers grow. Other past streptococcal infection tests have also been developed, such as the anti-CHO test which may help detect rheumatic fever.

Uploaded by

Myeonnie Kim
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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ACT 17

1. Describe other tests to detect RF. Discuss their principles

Other test to detect the Rheumatic factor are the following:

⮚ Latex agglutination method.

-It is a rapid latex agglutination test for the qualitative and semi-quantitative determination of
Rheumatoid Factor (anti-gamma globulin)

Principle:- Latex particles coated with human gamma globulins are allowed to react with test serum.
Agglutination will be observed if RF is present in the serum.

⮚ Haemagglutination test

-It is a type of agglutination test performed on red blood cells.

Principle:- It mixes the blood being tested with a sheep’s red blood cells that have been covered with
rabbit antibodies. If RF is present, the red blood cells agglutinate.

⮚ Nephelometry test

-A sensitive technique for measuring the degree of light scatter caused by the interaction of rheumatoid
factors with heat aggregated human IgG.

Principle:- Nephelometric testing of rheumatoid factor levels is based upon antigen-antibody reactions.
RF binds to IgG to form immune complexes. As the number of immune complexes grows, the solution
becomes more visibly “cloudy”.

⮚ Other tests to determine past streptococcal infections have been developed including the anti-
CHO test (possible use for rheumatic fever).

REFERENCES:

Naeva. (2014, August 6). Detection of rheumatoid factor by using latex agglutination. SlideServe.
Retrieved November 12, 2022, from https://www.slideserve.com/naeva/detection-of-rheumatoid-
factor-by-using-latexagglutination

RF direct product insert – cenogenics. (n.d.). Retrieved November 12, 2022, from
http://www.cenogenics.net/pdf/RF-Direct-Product-Insert1.pdf

editorial, B. M. G. (2022, October 21). Measuring rheumatoid factors with the NEPHELOstar: BMG
LABTECH. Retrieved November 12, 2022, from
https://www.bmglabtech.com/en/application-notes/measuring-of-rheumatoid-factors-by-the-
nephelostar-plus-microplate-reader/

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