DE LA SALLE UNIVERSITY

College of Science
Department of Biology

LBYBIO4-Vertebrate Embryology Laboratory

Prerequisite: COMPANA, LBYBIO3, LBYBIO1 Prerequisite to: none

Instructor: ______________________________ Contact details: ________________________

Consultation Hours: ________________________ Class Schedule and Room: ________________

Course Description

Vertebrate embryonic development is studied in the laboratory with frog, chick and pig as representative
animals. The course emphasizes actual observations of slide sections and integration with patterns and
mechanisms of development.

Learning Outcomes

On completion of this course, the student is expected to present the following learning outcomes in line with
the Expected Lasallian Graduate Attributes (ELGA).

ELGA Learning Outcome

Critical and Creative Thinker On the completion of the course, the student is expected
Effective Communicator to describe and identify the different stages of embryonic
Lifelong Learner development and compare and contrast the patterns of
Service-Driven Citizen development in vertebrates and also to further improve
their appreciation and respect of life.

Final Course Output

As evidence of attaining the above learning outcomes, the student is required to submit the following during
the indicated dates of the term.

Learning Outcome Required Output Due Date

On the completion of the course, the student Group Research Proposal Finals
is expected to describe and identify the Week
different stages of embryonic development
and compare and contrast the patterns of
development in vertebrates and also to
further improve their appreciation and
respect of life.

Rubric for assessment

A. Group Experimental Work and Presentation of Results

Neurula Gastrula Blastula Zygote

Criteria (3.5-4.0) (2.5-3.4)` (1.5-2.4) (1.0-1.4)
Objectives were set Objectives were set Objectives were set No objectives were
Set Objectives from the start of the from the start of from the start of set from the start of
(30%) experiment/presentatio the experiment/ the experiment/ the experiment/
nand were reviewed at presentation, and presentation, but presentation.
the end if it were were reviewed at were not reviewed
achieved. the end if it were at the end if it were
Moreover, the group achieved. achieved.
highlighted the
significance of the

2nd Term – AY 2018-2019

 reports of the course.

New information were New information New information No new

Content of learned from the was learned from was learned from information was
Experiment/ experiment/ the experiment/ the experiment/ learned from the
Presentation presentation, and was presentation and presentation but was experiment/
(30%) easy to understand. was easy to hard to understand. presentation.
Moreover, the understand.
presentation was
concise.
The group presented The group The group presented The group
their results clearly presented their their results but presented their
and made sure results clearly and lacked clarity and results very
Overall
everyone in class made sure fell short to make unclearly and made
Performance
understood the everyone in class sure everyone in no effort so
(20%) information they were understood the class understood the everyone in class
sharing. Moreover, information they information they understood the
they were very were sharing. were sharing. information they
enthusiastic and However they Enthusiasm and were sharing.
knowledgeable of their were not very knowledgeability Moreover, had no
report. enthusiastic or were low enthusiasm and
knowledgeable of were not
their report. knowledgeable of
their report.
The report was The report was The report was The report was dull
Multimedia creatively and creatively and ordinary and very and boring.
uniquely presented, uniquely standard, but was
(20%)
with the aid of the presented with the aided by the
computer. aid of the computer.
Moreover, it kept computer.
everyone attentive.

Additional Requirements

 Active individual participation in ALL laboratory activities.

 Submission of individual output or laboratory exercises.
 Participation in and submission of group output (Research Proposal)
 Five (5) Long Exams (Move Stations Type)

Grading System

Scale:

5 Long Exams 90% 92-100% 4.0

Group Research Proposal 5% 86-91% 3.5
Attendance/Performance 5% 80-85% 3.0
Total 100% 75-79% 2.5
Passing Grade 60% 70-74% 2.0
65-69% 1.5
60-64% 1.0
<60% 0.0

2nd Term – AY 2018-2019

Learning Plan

Learning Outcome Laboratory Activities Week Learning Activities

On the completion of the Mitosis
course, the student is Gametogenesis 1 Microscopic examination
expected to describe and Testis (Frog’s and mouse’s) of prepared slides
identify the different Ovary (Frog’s and cat’s)
stages of embryonic Stages of Early Ontogenic Development
development and Cleavage
compare and contrast the Blastula Microscopic examination
patterns of development Gastrula 2 of prepared slides
in vertebrates and also to Neurula
further improve their Neural plate
appreciation and respect Neural fold
of life. Neural tube
Amphibian Development
(Frog as animal model) 2 Microscopic examination
4 mm serial sections of prepared slides
10 mm serial sections
1st Long Exam 3 Move Stations Type
Avian Development
(Chick as animal model)
Whole-mounted samples 4 Microscopic examination
18 hr stage - wm of prepared slides
24 hr stage – wm
33 hr stage – wm
Serial section samples
24-hr serial sections
33-hr serial sections

Whole-mounted samples
48 hr stage – wm 5 Microscopic examination
72 hr stage – wm of prepared slides
96 hr stage – wm

2nd Long Exam 6 Move Stations Type

Serial section samples
48-hr serial sections 7 Microscopic examination
72-hr serial sections of prepared slides

3rd Long Exam 8 Move Stations Type

Mammalian Development 9-10
(Pig as animal model)
- Nervous System
- Digestive System
- Respiratory System

4th Long Exam 11 Move Stations Type

- Urogenital System
- Circulatory Sytem
Heart 12-13 Microscopic examination
Arterial System of prepared slides
Venous System

5h Long Exam 14 Move Stations Type

2nd Term – AY 2018-2019

 References

Appasani, K. and R. Appasani. 2011. Stem Cells & Regenerative Medicine (electronic resource) : From
Molecular Embryology to Tissue Engineering . Totowa, NJ: Humana Press
Desalle, R. and B. Schierwater. 2011. Key transactions in animal evolution. Enfield, New Hampshire:
Science Publishers
Kubiak, J. 2011. Cell Cycle in Development (electronic resource). Berlin, Heidelberg: Springer
Berlin Heidelberg.
Poole, D., Warren, A. and N. Nunez. 2007. The Story of human development. Upper Saddle River, NJ:
Pearson/Prentice Hall.
Schoenwolf, G. 2009. Laboratory studies of vertebrate and invertebrate embryos: guide and atlas of
descriptive and experimental development. San Francisco : Benjamin Cummings (REQUIRED TEXT)
Wolpert, L. and C. Tickle. 2011. Principles of Development. Oxford: Oxford University Press

Online Resources

For supplementary purposes only. Prelab lectures, the required text Schoenwolf, and the actual observations of
the slides in class will be the definitive bases of all the Long Exams.

Frog testis: http://home.cse.edu/~ikessler/emb/tutorials/frogtestis/indexfrogtestis.html

4mm frog: http://nte-serveur.univ-lyon1.fr/nte/embryon/www.uoguelph.ca/zoology/devobio/34mmfrog/
db34fg1.htm
10mm frog: http://nte-serveur.univ-lyon1.fr/nte/embryon/www.uoguelph.ca/zoology/devobio/57mmfrog/
db57fg1.htm
24hr chick: http://www.tulane.edu/~embryo/24hrChick/24hrChick.htm
33hr chick: http://www.tulane.edu/~embryo/33hrchick/33hrChick.htm
48hr chick: http://www.tulane.edu/~embryo/48hrchick/48hrMain.html
72hr chick: http://www.tulane.edu/~embryo/72hrChk/72htmlpages/72hrMain.html
10mm pig: http://www.tulane.edu/~embryo/10mmPig/10mmhtmlpages/10mmMain.html

Class Policies

 Honesty and integrity are integral components of the academic process. Any form plagiarism is
strongly prohibited.
 University policies on dishonesty, absences, and tardiness are strictly followed.
 Wearing of laboratory gown is required during laboratory period, as stipulated in policy on laboratory
classes.
 Proper decorum is to be observed among peers in all activities.
 Attentiveness and active participation are critical to successful learning.
 The use of cameras is allowed only on the class meeting immediately prior to the scheduled
examination (designated as the review session).
 No make-up exam for non-officially excused absence. Pass/Fail Policy will be observed for Vice Dean
certified absences, except for University sanctioned absences like Varsity competition.

Approved by:

DR. MARY JANE C. FLORES

Chair, Department of Biology

2nd Term – AY 2018-2019