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The document contains graphs showing calibration curves from analytical assays of various compounds using techniques like colorimetry, fluorimetry, and UV-spectrophotometry. The calibration curves plot absorbance, peak area, or fluorescence intensity against concentration and have high R-squared values, indicating a linear relationship between the measurement and concentration.

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60 views11 pages

Graphs - 1

The document contains graphs showing calibration curves from analytical assays of various compounds using techniques like colorimetry, fluorimetry, and UV-spectrophotometry. The calibration curves plot absorbance, peak area, or fluorescence intensity against concentration and have high R-squared values, indicating a linear relationship between the measurement and concentration.

Uploaded by

mr copy xerox
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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0 0

0.5 0.099
1 0.612
1.5 1.122
2 1.616
2.5 2.062

ASSAY OF SULPHANILAMIDE USING BRATTON MARSHALL


REAGENT
2.5

2
ABSORBANCE

1.5

y = 0.986x - 0.3768
1 R² = 0.9993

0.5

0
0 0.5 1 1.5 2 2.5 3

CONCENTRATION(mcg/ml)
0 0
0.4 0.38
0.6 0.45
0.8 0.501
1 0.589

CIPROFLOXACIN BY COLORIMETRY METHOD


0.7

0.6

0.5
ABSORBANCE

0.4
y = 0.339x + 0.2427
0.3 R² = 0.9898
0.2

0.1

0
0 0.5 1 1.5
CONCENTRATION(mcg/ml)
0 0
10 0.46
20 0.83
30 1.08
40 1.29
50 1.512

RIBOFLAVIN BY COLORIMETRY METHOD


1.8
1.6
1.4
1.2
ABSORBANCE

1
0.8 y = 0.0256x + 0.2652
0.6 R² = 0.9853
0.4
0.2
0
0 20 40 60
CONCENTRATION(mcg/ml)
0 0
100 0.316
200 0.527
300 0.713
400 0.896
500 1.017

IBUPROFEN-UV SPECTROPHOTOMETRY
1.2

1
ABSORBANCE

0.8

0.6 y = 0.0018x + 0.1625


R² = 0.9917
0.4

0.2

0
0 200 400 600
CONCENTRATION9mcg/ml)
0 0
5 0.457
10 0.768
15 1.045
20 1.568
25 1.865
30 2.264

CALIBRATION CURVE
2.5

2
ABSORBANCE

1.5

1
y = 0.0734x + 0.0429
R² = 0.9933
0.5

0
0 5 10 15 20 25 30 35
CONCENTRATION(mcg/ml)
0 0
400 0.1
410 0.057
420 0.042
430 0.014
440 0.077
450 0.129
460 0.189
470 0.349
480 0.486
490 0.731
500 0.974
510 1.218
520 1.35
530 1.536
540 1.437
550 1.447
560 0.934
570 0.867
580 0.478
590 0.217

1.8
1.6
1.4
1.2
ABSORBANCE

1
0.8
0.6
0.4
0.2
0
0 100 200 300 400 500 600 700
WAVELENGTH
0 0
1 50
2 56.6
3 62.5
4 69.2
5 74.7
6 79.7
7 85.8
8 90.2
9 93.3
10 100

QUININE SULPHATE BY FLUORIMETRY


120

100

80
ABORBANCE

60

y = 5.4558x + 51.649
40
R² = 0.9947
20

0
0 2 4 6 8 10 12
CONCENTRATION(ppm)
0 0
10 567458
20 1174123
30 1806775
40 2269832
50 2837290

STANDARD CURVE OF DICLOFENAC SODIUM


4000000

3500000

3000000

2500000
PEAK AREA

2000000

1500000 y = 56354x + 604021


R² = 0.9974
1000000

500000

0
0 10 20 30 40 50 60
CONCENTERATION(mcg/ml)
0 0
2.5 831253
5 1694215
7.5 2532451
10 3323412
12.5 4125435
15 4927209

STANDARD CURVE OF ACECLOFENAC


6000000

5000000

4000000
PEAK AREA

3000000

2000000 y = 326450x + 49222


R² = 0.9997
1000000

0
0 2 4 6 8 10 12 14 16
CONCENTERATION(mcg/ml)
0 0
6 51476
8 67598
10 84897
12 101114
14 119554

STANDARD CURVE OF PARACETAMOL


140000

120000

100000
PEAK AREA

80000
y = 8483.6x + 91.8
60000 R² = 0.9995
40000

20000

0
0 2 4 6 8 10 12 14 16
CONCENTERATION(mcg/ml)
0 0
0 1.4
1 1.9
2 1.4
3 1
4 0.5
5 0.1

QUENCHING OF QUININE SULPHATE BY KI USING


FLUORIMETRY
2
FLUORESCENCE INTENSITY

y = -0.3171x + 1.8429
1.5 R² = 0.8093

0.5

0
0 1 2 3 4 5 6
VOLUME OF KI

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