MANILA TYTANA COLLEGES
SENIOR HIGH SCHOOL DEPARTMENT
Pres. Diosdado Macapagal Blvd., Metropolitan Park, Pasay City
GENERAL BIOLOGY 2
Manipulation of Compound Microscope
Laboratory Experiment No. 1
Members: ______________________________________________________ Group Number: ___________________
Grade and Section: _______________________________________________ Date: ____________________________
OBJECTIVES
● To identify the parts of a compound microscope and relate them to their functions
● To compute the total magnification provided by the compound microscope.
● To illustrate, describe and differentiate the different animal tissues.
SPECIMENS
Small letter “e”
Animal tissues (blood smear, smooth muscle, stratified squamous epithelium)
MATERIALS
Compound microscope, ballpen, pencil, laboratory gown
PROCEDURE
1. Borrow a compound microscope from the Science Laboratory. Carry it carefully.
2. Place the microscope on the laboratory table just in front of you.
3. Clean the ocular and objective lenses with the lens paper for a clear view of your specimen.
4. Look at the ocular/eyepiece and notice a number with an “x” after it. This refers to the magnification of the
eyepiece. If it has 5x, it means a magnification of five times the original size.
5. Look for the objective lenses. There may be three or four of them. You will notice that each objective has a number
followed by “x”. Each objective magnifies the object by the factor marked on the particular lens. For example, LPO
has 10x, HPO has 40x, and oil immersion has 100x, which gives the largest images. If you multiply the magnification
of the ocular by the magnification of the objective being used, you will arrive at the total magnification of the
microscope. For example, if you are using the LPO, which has 10x, just multiply this with the magnification number
from the ocular, which is 10x, and you will get a total magnification of 100x.
6. Use the prepared slides of letter “e” and animal tissues and observe under a compound microscope.
RESULTS
1. Draw the letter “e” as seen under LPO and HPO
LPO HPO
2. Draw three (3) animal tissues as seen under LPO and HPO. Label the visible parts.
A. Specimen: ________________________
LPO HPO
B. Specimen: ________________________
LPO HPO
C. Specimen: ________________________
LPO HPO
QUESTIONS
1. Parts
a. What part of the microscope concentrates the light?
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b. What regulates the amount of light in the microscope? Why do you need to regulate it?
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c. What will you use to clean the lenses of the microscope? Why would you use this?
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2. Manipulation
Letter “e”
a. What happened to the position of the letter “e” as seen under the microscope?
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b. With which objective did you see the whole mount of the letter “e”? LPO or HPO?
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Animal Tissues
a. Can you see the whole animal tissue under LPO? HPO?
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b. What are the functions of epithelial tissues?
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c. What is/are the components of the extracellular matrix of the blood?
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d. Differentiate skeletal, cardiac, and smooth muscle.
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3. Magnification
Complete the table below by supplying the missing magnifications of the objective lenses and total magnification.
Ocular Objective Total Magnification
10x 100x
10x 400x
10x 100x
10x 5x
4. Usage
a. Why must you use the fine focus control for HPO?
________________________________________________________________________________________
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b. Why do you have to make sure that the area you wish to study is in the center of the field of view before
changing the objective?
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c. Why must you turn the nosepiece to the LPO before putting the microscope away?
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