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Biological Microscopes 2005 - 2

The document discusses Olympus biological microscopes and their capabilities. It introduces the FluoView FV1000 and FV300 confocal laser scanning biological microscopes, which offer high resolution confocal sectioning and the ability to conduct time-lapse experiments. It also describes the BX61 motorized system microscope and various other Olympus microscope models, objectives, cameras, and accessories for biological and medical research applications.
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0% found this document useful (0 votes)
137 views24 pages

Biological Microscopes 2005 - 2

The document discusses Olympus biological microscopes and their capabilities. It introduces the FluoView FV1000 and FV300 confocal laser scanning biological microscopes, which offer high resolution confocal sectioning and the ability to conduct time-lapse experiments. It also describes the BX61 motorized system microscope and various other Olympus microscope models, objectives, cameras, and accessories for biological and medical research applications.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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BIOLOGICAL

MICROSCOPES

2005-2
N ew avenues of research are opening in biological and medical

fields. As research demands become more specialized and

diversified, biological microscopes must offer the capabilities to

meet these needs.

Olympus microscopes and their accessories are developed to meet

the ever-changing needs of research applications.

Our accomplishments in microscope development date back more

than three-quarters of a century. Olympus has accumulated a broad

range of advanced optical and precision technologies and we are

renowned for our innovative approach to microscopy.

An outstanding example of Olympus ingenuity is the superior UIS2

infinity-corrected optical system employed on the BX2 and IX2

microscopes. Olympus has also won acclaim for its system

versatility and broad range of advanced accessories.

Our microscopes are evolving with enhanced performance and

operational ease. Olympus continues to answer research demands in

the biological and medical field of today and pave the way for future

advances with increasingly sophisticated research equipment.


Contents
Fluoview FV1000 Confocal Laser Scanning Biological Microscope ... 3
Fluoview FV300 Confocal Laser Scanning Biological Microscope ... 3

BX61 Motorized System Microscope ... 4


BX51 System Microscope ... 4
BX41 System Microscope ... 5
BX45/BX45A Ergonomic Laboratory Microscope ... 5
Accessories for BX2 ... 6-7

BX51WI Fixed Stage Upright Microscope/


BX61WI Fixed Stage Upright Microscope with Motorized Focusing ... 7

CX41 System Microscope/CX31 Biological Microscope ... 8


CX21 Biological Microscope ... 8

IX81 Motorized System Inverted Microscope ... 9


IX71 Resarch Inverted System Microscope/
IX51 Inverted System Microscope ... 9
Accessories for IX2 ... 10
CKX41/CKX31 Inverted Microscopes ... 11
Accessories for CKX41/CKX31 ... 11

ON3 Series Micromanipulators ... 12

BX51-P Polarizing Microscope ... 13


CX31-P Polarizing Microscope ... 13

SZX12/SZX9 Research Stereomicroscope System ... 14


SZX7 Stereomicroscope System ... 15
SZ61/SZ51 Stereomicroscope System ... 15
SD/SF Series Stereo Microscopes ... 15
Accessories for SZX/SZ ... 16

DP70 Digital Camera ... 17


DP30BW High Sensitivity cooled CCD camera ... 17
DP12 Microscope Digital Camera ... 17
PM30 Automatic Photomicrographic System ... 18
PM20 Automatic Photomicrographic System ... 18
PM10SP Automatic Photomicrographic System ... 18

UIS2 Series Objectives ... 19-21


UIS Series Objectives ... 20, 22
The FV1000 is the original spectral detection system uses a high

FLUOVIEW FV1000
speed diffraction grating in combination with a variable slit to
deliver superior linear spectral distribution, enabling high-precision,
high-resolution,high-speed spectroscopy in observations ranging
CONFOCAL LASER SCANNING
from milliseconds to hours. SIM (SIMultaneous) Scanner System
BIOLOGICAL MICROSCOPE Synchronizes laser light stimulation and confocal imaging.
The FV1000 incorporates 2 laser scanners for simultaneous
observation and laser light stimulation.
The FV1000 the most suitable choice of microscope for FRAP,
FLIP and photo activation.
UNIVERSAL
INFINITY SYSTEM

Spectral type fluorescence detector Filter type fluorescence detector


Laser Visible Multi-line Ar laser ,HeNe(G) laser, HeNe (R) laser
light light HeNe(G) laser (543nm, 1mW), HeNe (R) laser(633nm, 10mW)
laser Visible light laser platform with implemented AOTF system
Violet laser (option)
Laser Diode 405nm, 440nm
Laser port Standard 3 laser ports, VIS - UV - IR
Scanning Detector Standard 3 confocal Channels. Standard 3 confocal Channels.
and module Spectral detection : CH1 to CH3 each with 6 position
Detection CH1 and CH2 equipped with barrier filter turret.
independent grating.
CH3 with 6 position barrier filter turret
Scanning method Dual galvano mirror scanner (X, Y)
Scanning pixel size:64 x 64 — 4096 x 4096
modes Pixel Dwell time: 2 to 200 microsec with unidirectional
0.5 or 1 microsec with fast bidirectional scanning
X,Y,T,Z,λ (any combination)
Line scanning: Straight line with free orientation, free line
Field Number (N.A.) 18
Optical Zoom 1X — 50X in 0.5X increment
Z-drive Motorized focus module of the microscope, minimum increment 10 nm
Transmitted External transmitted photomultiplyer detector
light detector unit
Micro- Motorized Inverted IX81, Upright BX61,
scope microscope Upright focussing nosepiece & fixed stage BX61WI
Option- SIM 2 Galvano scanning mirrors, pupil projection lens,
al Scanner built-in laser shutter, 1 laser port
unit Fiber introduction of near UV laser diode or visible light laser,
FV1000+IX81 Optional: 2nd AOTF laser combiner
*Please refer to FV1000 catalog for further details

Giving both individual and group users the right solution to

FLUOVIEW FV300
match their research needs and budget.
The systems is compatible with the Olympus research range of
microscopes offering high resolution confocal sectioning with the
CONFOCAL LASER SCANNING
ability to conduct time-lapse experiments.
BIOLOGICAL MICROSCOPE The FV300 offers a wider number of options with the ability to
upgrade the systems for the future.
•Highest image quality (12 bit, 2048x2048 pixel resolution) with
economical cost.
•Simultaneous capturing of 2 fluorescence and 1 transmitted light
UNIVERSAL
INFINITY SYSTEM
detector images.
•Simple, straight optical systems for easy system construction.

Up to 3 channel *2-channel fluorescence (+1-channel transmitted light)


Visible light laser, *Ar laser, *Multi laser, *Green HeNe laser, *Red HeNe laser,
Helium Cadmium laser
1 laser port for visible light laser
Manual operating scanning unit
5 position single pinhole turret
Scanning unit, *Galvanometer mirror scanner (both X and Y), *Photo detector: PMT
Automatic laser control/laser combiner
*Each laser light path is equipped with countiunuousy variable neutal density filters
or AOTF and a shutter
All lser lines are combined and introodued into via a single fiber optic
Intuitive user friendly software
Scanning mode: 1-dimension: Point scanning
2-dimension (space): X-Y, rect, X-Z, linear line-Z
and free line-Z
2-dimension (time): X-t, linear line-t and free line-t
3-dimension (space): X-Y-Z and rect-Z
3-dimension (time): X-Y-t, X-Z-t and rect-t
4-dimension: X-Y-Z-t and rect-Z-t
Image size and scanning speed: 256X256(0.45s) - 2048X2048(10.835s)
*High speed mode: 512X512(0.25s)
Image acquisition: 12bit
Image analysis: 2D measurement, image filtering,
various image display mode,
3D animation display etc.
Microscope
Upright microscope (BX50, BX51, BX61), Inverted microscope (IX70, IX71, IX81),
Fixed stage upright microscope (BX61WI)
FV300+BX61WI
*Please refer to FV300 catalog for further details
Motorized system microscope BX61 in the BX2 series features
outstanding reliability in high advanced microscopy such as three
dimensional imaging capturing with high-end fluorescence imaging
MOTORIZED SYSTEM MICROSCOPE and confocal microscope.
Standard features include motorized focusing and light adjustment
and a stage escape mechanism. Several key macro microscope
operations are available by software-controlled setting, and
executed by hand switch, personal computer.

•Uses flexible software which can easily be customized for specific


purposes.
•Many operating procedures (including switching between observation
methods) are stored as individual macros and allotted to buttons on the
microscope, hand switches and keys on the monitor of the personal
computer.
•A wide variety of separate modules make expansion very easy.

Illumination Transmitted light 12V100W halogen Koehler illumination


Focusing Motorized focus
full stroke: 14mm, minimum fine adjustment: 0.01µm
Observation tube Widefield binolucar (F.N.22), widefield tilting binocular (F.N.22),
widefield trinocular (F.N.22), widefield tilting/telescoping binocular
(F.N.22), super widefield trinocular (F.N.26.5)
Nosepiece Interchangeable reversed quintuple/sextuple/septuple, motorized
sextuple with slider slot for DIC, septuple for DIC/simple POL
Stage Ceramic-coated coaxial with left or right hand low drive control,
non-stick grooved coaxial, plain, rotatable
Condenser Abbe (N.A.1.1), swing out Achromatic (N.A.0.9),
Achromatic Aplanatic (N.A.1.4), universal (N.A.1.4/0.9)
Other features Coarse/fine changeover button, stage shunting button, stage
up/down button, built-in filters (LBD-IF, ND6, ND25, option)
Accessories Motorized fluorescence illuminator, motorized universal
condenser, motorized transmitted filter wheel, motorized
reflected filter wheel, motorized observation filter wheel,
hand switch, control unit, etc.
*Please refer to BX51/BX61 catalog for further details

These leading models from the BX2 series offer improved


ergonomics and system performance, and are widely used in both
routine work and specialized research. Each is equipped with
Olympus’ original UIS2 optic system, and a high-rigidity Y-shape
SYSTEM MICROSCOPE frame with newly refined ergonomics. Excellent features provide
the flexibility needed for compliance with a variety of applications.
In addition, many kinds of filter sliders and accessories are all
designed with multiple opening on the bodies and illuminators.

•Can be combined with a swing-out condenser and a septuple revolving


nosepiece with DIC slider to enable continuous observations from 1.25X
to 100X.
•A newly developed 8-position universal condenser gives even greater
freedom to combine observation methods.

Illumination Transmitted light 12V100W halogen Koehler illumination


Focusing Coarse & fine coaxial handle; full stroke: 25mm,
minimum fine adjustment: 1µm
Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22),
widefield trinocular (F.N.22), widefield tilting/telescoping binocular
(F.N.22), super widefield trinocular (F.N.26.5)
Nosepiece Interchangeable reversed quintuple/sextuple/septuple
Stage Ceramic-coated coaxial with left or right hand low drive control,
non-stick grooved coaxial, plain, rotatable
Condenser Abbe (N.A.1.1), swing out Achromatic (N.A.0.9),
Achromatic Aplanatic (N.A.1.4), universal (N.A.1.4/0.9)
Other features Coarse/fine changeover button, stage shunting button, stage
up/down button, built-in filters (LBD-IF, ND6, ND25, option)
Accessories Reflected light fluorescence attachment, DIC attachment,
phase-contrast attachment, multi-viewing attachment,
polarizing attachment, etc.
*Please refer to BX51/BX61 catalog for further details
This key model in the BX2 series offers an excellent cost-
performance and meets a wide variety of needs in the routine
inspection of laboratories and clinics. The extensive range of
features benefits from the consistently simple operation, enhanced
SYSTEM MICROSCOPE by Olympus’ original and ergonomically-advanced Y-shape frame
design and UIS2 optic system. This model allows easy system
expansion: by combining multiple units together, it can meet the
majority of general working requirements.
•Newly developed 30W halogen light source provides as much brightness
as a conventional 50W halogen lamp.
•Equipped with an easy-to-operate rackless stage with no protrusion of
the X-axis guide.
•The front-located power switch and light intensity volume control make it
possible to operate the microscope with the arms resting on the desk.
•A 3-filter cassette enables quick, easy exchange.
•The detachable revolving nosepiece allows quick exchange of objectives
to suit different observation methods.

Illumination Transmitted light 6V30W halogen Koehler illumination


Focusing Vertical stage movement: 25mm; Stage stroke with coarse
adjustment limit stop; Torque adjustment for coarse adjustment
knobs; Stage mounting position variable; High sensitivity fine
focusing knob (adjustment graduations 1µm)
Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22),
widefield trinocular (F.N.22), widefield ergo binocular (F.N.22)
super widefield trinocular (F.N.26.5)
Nosepiece Interchangeable reversed sextuple/quintuple
Stage Ceramic coated coaxial with right or left hand low drive control
and rotating mechanism, non stick grooved coaxial, plain,
rotatable
Condenser Abbe(N.A.1.1), swing-out Achromat (N.A.0.9),
Achromatic Aplanat (N.A.1.4), phase-contrast/darkfield (N.A.1.1),
darkfield dry (N.A,0.8-0.92), darkfield oil (N.A.1,20-1,40),
ultra low (N.A.0.16)
Other features Light pre-set switch
Accessories Reflected light fluorescence attachment, DIC attachment,
phase-contrast attachment, multi-viewing attachment, etc.
*Please refer to BX41/BX45/BX45A/BX51 catalog for further details

These high-performance laboratory microscopes are equipped


with the excellent image quality UIS2 optics and ergonomically
advanced Y-shape frame as other models in the BX2 series.
ERGONOMIC •Uses no-cover objectives which are suitable for observing smear
LABORATORY MICROSCOPE specimens (e.g. blood) with no cover glass.
•Includes a 10x objective with built-in ND filter. The brightness remains the
same even when magnification is increased to 40x.
•Employs a rackless stage providing precise movement and smooth
stopping with no protrusion of the X-axis guide.
•A convenient marking unit is provided.

BX45 -------------------------------------------------------------------------------------------------------------------------------
A 3-position universal condenser which complies with brightfield,
phase-contrast and darkfield observations, and a gout inspection
analyzer that uses simple polarizing observation. Possible to get
optimal illumination from 1.25x to 40x observation.
BX45A ---------------------------------------------------------------------------------------------------------------------------
A low stage position fixed at 128mm from the desktop, revolving
nosepiece with variable up/down movement, and an motorized
2-position revolving nosepiece which can make quick changes
between objectives.

Illumination Transmitted light 6V30W halogen Koehler illumination


Focusing Fixed low stage nosepiece focus
Observation tube Widefield binocular (F.N.22), widefield tilting binocular (F.N.22),
widefield trinocular (F.N.22), widefield ergo binocular (F.N.22)
Nosepiece Fixed motorized 2-position (BX45A)
Fixed reversed quintuple (BX45)
Stage Ceramic-coated coaxial with right or left hand low drive control,
rotating and torque adjustment mechanism, plain, rotation
Condenser Brightfield N.A.0.9 (BX45A)
Fixed 3 position universal condenser N.A.0.9 (BX45)
Other features One-touch marking unit, Light preset switch,
Torque adjustment
BX45 BX45A
*Please refer to BX41/BX45/BX45A/BX51 catalog for further details
Accessories for BX2

BX-RFAA U-FWR, U-FWO, U-FWT U-UCD8A-2


Motorized fluorescence illuminator Filter wheels Motorized universal condenser
Up to 6 fluorescence mirror units can be Motorized exchange of 6 filters. 3 kinds of Different combinations of designated
attached simultaneously. Mirror unit can filters can be attached simultaneously: optical components allow correspondence
be exchanged automatically with U-FWR (ø32, 25) for excitation, with various kinds of transmitted light
corresponding shutter adjustment. U-FWO (ø32, 25) for absorption and observation. Automatic control of optical
U-FWT (ø32) for transmitted light. component exchange, top lens swing
in/out and aperture iris diaphragm.

U-AFP1 U-UCD8-2 U-PCD2


Auto focus unit 8 position universal condenser Phase/darkfield condenser
Maintains steady auto focusing with 1.25X The universal condenser simultaneously High contrast phase imaging allows close
to 100X objectives. Applicable observation accepts up to 6 DIC prisms and 2 other observation of the cell interior and of live
methods are all besides phase-contrast. optical components at maximum. bacteria. Standard brightfield and dry
Combinable microscopes are BX61TRF The condenser numerical aperture of 0.9 darkfield observations and simultaneous
and BX62TRF. A host personal computer or 1.4 (oil) can be selected through observation of reflected light fluorescence 6
and BX2-UCB are required. interchangeable top elements. are possible.

U-KPA

U-POT U-ANT

BX-RFA, BX-URA2 U-RFSS U-KPO


BX fluorescence illuminator Rectangular field stop Simple polarizing attachment
BX reflected light illuminator Designed for use with CCD cameras, Simple polarizing observation can be
Fully integrated into the microscope arm, prevents photobleaching of the specimen accomplished with the combination of U-
two illuminators add to the stability of the outside of the imaging area. KPA intermediate attachment for simple
imaging platform. BX-RFA for research polarizing observation, U-ANT analyzer for
needs and BX-URA2 for routine observation. transmitted light and U-POT polarizer.

U-RSL6, U-RSL6EM U-EXBABG, U-EXBAUB, U-EXBAUG U-LH50MH


6 position filter slider Excitation balancer Metal halide lamp housing
Equipped with three single, two dual and Used singly or in tandem, the excitation This 50W lamp house offers low cost in
one triple band exciter, the 6 position filter balancer curtail the individual excitation long time usage and suitable for
slider provides all excitation modes bandwidths of the fluorochromes under experiments such as multicolor time lapse
typically wanted with just one filter set. observation. and ratio imaging.
Accessories for BX2
U-SDO3, U-MDO10B3
Multi observation body
A single image can be viewed with
the same orientation and brightness
by up to ten persons simultaneously
to facilitate discussion between
researchers, and for training and
education. No additional power unit is
required since the power unit of the arrow
pointer is incorporated in the main body.
•Also available for five persons (U-MDO) and
two persons in face-to-face (U-BDO).

BX51+U-MDO10B3

BX51+U-SDO3

The BX51WI is designed for Neuroscience and Cell Biology


application with incorporating the front focus operation and also
features total vibration free operation even during switching the
FIXED STAGE UPRIGHT MICROSCOPE/ objectives on the nosepiece and filters on the turret and optical
FIXED STAGE UPRIGHT MICROSCOPE path on the intermediate tubes. Two-wave length IR-DIC (775nm
WITH MOTORIZED FOCUSING and 900nm) has huge advantage for researchers whose work for
electro-physiology with brain slice sample. Our unique two-place
nosepiece can easily switch the objectives and then prevent the air
bubbles at the top of them. In addition Olympus offers only one
objective concept incorporated with XLUMPLFL20X/W objective
UNIVERSAL
INFINITY SYSTEM
and the intermediate magnification changer. BX51WI provides
dramatic bright fluorescence image along with our BX2 family.

Illumination Transmitted light 12V100W halogen Koehler illumination (BX51WI)


Transmitted light 12V100W halogen Koehler illumination;
Light adjustment: less than DC2Vg12V (continuous adjustment)
Brightness adjustment, light preset switch (BX61WI)
Focusing Nosepiece focus by roller guide (rack & pinion);
Stroke per rotation: fine: 0.1mm, coarse:15mm;
Maximum stroke: 25mm; Coarse lower limit stopper mechanism,
Torque adjustment mechanism for coarse focus (BX51WI)
Motorized focusing using stepping motor and ball screw
Nosepiece focus by cross roller guide;
Minimum graduation; fine: 1µm (sensitivity 1µm)
Resolution 0.01µm: Maximum stage movement speed 3mm/s
Stroke: 25mm, stage escape mechanism (BX61WI)
Observation tube Trinocular (F.N.22), erect image trinocular (F.N.22),
double port magnification change unit (F.N.22)
Nosepiece Swing, slide, single, position, swing-slide
Stage Mechanical, bridge
Condenser 8 position universal, long working distance oblique,
long working distance DIC, swing-out
*Please refer to BX51WI/BX61WI catalog for further details
The CX41/CX31 offer extended capabilities to match a wide range
of applications from routine clinical work to educational use. They
not only feature an ergonomically designed frame for maximum
SYSTEM MICROSCOPE/ operating comfort and enhanced rigidity, but also offer the
convenience of extra-bright illumination.
BIOLOGICAL MICROSCOPE
CX41 ------------------------------------------------------------------------------------------------------------------------------
Featuring powerful 6V30W halogen Koehler illumination and
outstanding flat images in this class of microscope, the CX41 is
applicable for a wide range of observation methods and
photomicroscopy.
CX31 ------------------------------------------------------------------------------------------------------------------------------
An ergonomically designed frame and bright 6V30W halogen
illumination make the CX31 ideal for routine clinical work and
educational applications.

Illumination Built-in transmitted Koehler illuminator


6V30W halogen bulb
100-120V/220-240Vg 0.85/0.45A 50/60Hz
Focusing Stage height movement by roller guide (rack & pinion)
Stroke per rotation: 36.8mm
Full stroke range: 25mm
Tension adjustment on coarse focus adjustment knob
Upper limit stopper (CX41)
Upper limit stopped by simplified pre-focusing dial (CX31)
Observation tube Binocular/Trinocular/Tilting trinocular
Nosepiece Fixed quadruple nosepiece with inward tilt
Stage Size: 188(W) X 134 (Y) mm
Movement range: (Y) 50mm X (X) 76mm
Specimen holder: Double slide holder
Condenser Abbe condenser, with built-in daylight filter(CX31 only)
CX31 Accessories Dual-observation attachment, phase-contrast attachment,
drawing attachment, simple polarizing attachment,
digital camera adapter etc.
CX41 *Please refer to CX41, CX31 catalogs for further details

The CX21 demonstrates the ideal combination of advanced


performance and operability for multiple inspection and
educational purposes in the medical field. Incorporating the UIS2
BIOLOGICAL MICROSCOPE optical system and employing Plan objective lenses as standard,
it delivers class-leading standards of image clarity and flatness in
a wide range of observation methods.
Other characteristics include excellent durability, and ergonomic
design features to reduce fatigue during long observations.
To maintain performance in any working environment, an effective
anti-fungal treatment is applied to the objectives, eyepieces and
microscope tube.

Optical system UIS2 (Universal Infinity System) optical system


Illumination System Built-in transmitted illumination system
6V20W halogen bulb
100-240V 50/60Hz universal voltage
Focusing Stage height movement (coarse movement stroke 20mm)
Fine focus graduation: 2.5µm
Revolving nosepiece Fixed quadruple nosepiece
Stage Wire movement mechanical fixed stage: 120 X 132mm
Traveling range: 76mm(X) X 30mm(Y)
Single specimen holder
Observation tube 30° inclined binocular tube
Interpupillary distance adjustment range 48-75mm
Condenser Abbe type with aperture iris diaphragm N.A.: 1.25
Objective lens Plan Achromatic objectives (anti-fungus)
4X N.A.: 0.10 W.D.: 18.5mm
10X N.A.: 0.25 W.D.: 10.6mm
40X N.A.: 0.65 W.D.: 0.6mm
100X N.A.: 1.25 W.D.: 0.13mm (option)
Eyepiece (10X) Field Number (F.N.): 18 (anti-fungus)
Optional accessories Mirror unit, 15X eyepiece (F.N. 12, anti-fungus), cord rest,
wooden storage box, filar micrometer, wire pointer,
filter holder, darkfield stop
*Please refer to CX21 catalog for further details
This model allows researchers to customize the motorized system
according to their own specific purpose, with operating control
handled from the front. By using special software via a personal
computer, it is also possible to exercise accurate control of multi-
MOTORIZED SYSTEM dimensional analyses, ranging from 2D to 6D. The full range of
INVERTED MICROSCOPE IX81performance functions, including observations, measurements
and manipulation, can be monitored via the numerous
input/output ports, which allow connection of various kind of light
sources and motorized modules.

•Purpose-selectable motorized units and easy operation right by the


operator's hand.
•Multi-dimensional analysis by PC control.
•Maximum installation of experimental equipment and minimum layout
limitations.
•Sharp, fade-free fluorescent images and faster observations.
•Optimized resolution and contrast in Nomarski DIC observation, for both
thick- and thin-cell specimens.
•Combining different light sources and video systems to obtain even
clearer images.
•Prolonged active-cell observation with highly reliable data.
•Special microscope body for FV1000/300 is available.

Illumination Transmitted Koehler light 12V100W halogen


Focusing Motorized focus; Stroke: 9mm Resolution: 0.1µm
Observation tube Tilting binocular (F.N. 22), trinocular (F.N. 22)
Nosepiece Motorized sextuple with simple waterproof mechanism
Stage Cross with flexible right handle, plain, mechanical
Condenser Motorized long working distance universal,
long working distance universal, DIC, mid long working distance,
8 position universal, ultra long working distance universal
Other features Video port for primary image,
integrated magnification change lens
Accessories Motorized/manual reflected light fluorescence attachment, DIC
attachment, external power supply unit, side-viewing attachment,
incubator, heat stage, micromanipulator, etc.
*Please refer to IX71/IX81 catalog for further details

Designed to provide the high performance and versatility needed


by researchers involved in live cell experiments, the IX71 offers
highly precise temperature control and resistance to heat and
RESEARCH INVERTED SYSTEM MICROSCOPE/ vibration, enabling work on active cells with much less risk of
damage and reducing the incidence of failure in prolonged
INVERTED SYSTEM MICROSCOPE experiments.
A 30W illumination pillar type IX51, which has no intermediate
magnification changer, is also available.

•More free space and a better working environment, with flexible use of
several cameras and light sources.
•Easy front operation allows auxiliary equipment to be placed near the
microscope.
•Flexible system expansion allows many different fluorescence applications
without major remodeling.
•Obtaining high-quality, purpose-specific images with different cells and
different types of container.
•Rigid construction and comprehensive system features to analyze time
lapse changes in highly active cell conditions.
•Special microscope body for FV1000/300 is available.

Illumination Transmitted light 6V30W halogen illumination


Focusing Vertical movement of nosepiece (stage fixed);
coarse & fine coaxial handle; full stroke: 9mm;
minimum fine adjustment: 1.2µm
Observation tube Tilting binocular (F.N.22), binocular (F.N.22), trinocular (F.N.22)
Nosepiece Sextuple, simple waterproof mechanism
Stage Cross with flexible right handle, cross with short left handle,
plain, mechanical
Condenser Motorized long working distance universal,
long working distance universal, DIC,
mid long working distance, 8 position universal
IX51 ultra long working distance universal
Other features Video port for primary image,
integrated magnification change lens
Accessories Reflected light fluorescence attachment, DIC attachment,
external power supply unit, side-viewing attachment, incubator,
IX71 heat stage, micromanipulator, etc.
*Please refer to IX71/IX81 catalog and IX51 catalog for further details
Accessories for IX2

TIRFM
Total internal reflected
fluorescent microscopy
An exclusive high N.A.(1.65,1.45)
U-DPCAD
Dual port with C-mount adapter
objective and reflected light
illuminator are provided, allowing Simultaneous mount of two cameras on
exchange between Evanescent left side port possible.
wave and normal fluorescence Setting example: IX71 TIRFM version
observation.

IX2-RFA IX2-RFAL IX2-GS


Fluorescence illuminator L-shape fluorescence illuminator Gliding stage
Can be mounted with six different mirror Allows easy centering and AS/FS To follow the quick movement of
units. An original mirror unit can be tailor- operation from the front and also permits caenorhabditis elegance, this stage is
made from generic mirror units. UV cut attachment of a large format camera to designed move smoothly and freely
filter integrated. the back port. throughout the plane.
10

IX2-LWUCD IX2-MLWCD IX2-DICD+IX2-TLW


Long working distance universal Mid long working distance DIC condenser +
condenser condenser water top lens for DICD
The new DIC system is especially effective in Olympus’ modulation contrast equipment, By combining the IX2-TLW top lens, DIC
obtaining high-contrast, high-resolution which can cast a shadow in any prism and DIC slider, this model provides
images in 20x and 40x DIC observations. designated direction. excellent operability in injection and patch
clamping operations.

IX2-TVRAC IX2-RFACA IX2-LWUCDA2


Motorized bottom port unit with Motorized fluorescent turret Motorized long working distance
C-mount universal condenser
Motorized change between reflected light
Entirely aberration-free primary images fluorescence and transmitted DIC Simultaneously accepts up to 6 optical
from UIS2 objectives are directed to c- observations possible. Can be mounted components at maximum. Motorized
mount CCD camera. with 6 mirror units. exchange through PC possible.
The CKX41/CKX31 are designed to achieve less time consuming
cultured cell viability checking. An unique centering free/common
phase annulus for 10x, 20x and 40x provides faster phase-
INVERTED MICROSCOPES contrast observation without adjustment. The compact but solid
frame design can save space and best fit into clean bench.
CKX41 ---------------------------------------------------------------------------------------------------------------------------
Observation tube is exchangeable, a trinocular tube is also
mountable. A glass stage insert plate provides quick recognition of
objectives.
CKX31 ---------------------------------------------------------------------------------------------------------------------------
The CKX31 is a standard type with a fixed binocular tube and a
powerful 6V30W halogen illumination and ideal for routine cultured
cell observation.

Illumination Transmitted light 6V30W halogen illumination


Focusing Vertical movement of nosepiece (stage fixed);
coarse & fine coaxial handle; full stroke: 9mm;
minimum fine adjustment: 1.2µm
Observation tube Binocular (CKX31 frame/CKX41 frame with tilting binocular tube)
Trinocular (CKX41 frame with trinocular tube)
Nosepiece Fixed quintuple
Stage Plain stage (160mm X 240mm), attachable mechanical stage
CKX31 Condenser Detachable long working distance condenser
(N.A. 0.3, W.D. 72mm)
CKX41 Accessories Glass stage insert plate, eyepoint adjuster, Relief Contrast
system, Terasaki holder, 35mm dia., petri dish holder, slide glass
holder, etc.
*Please refer to CKX41/CKX31 catalog for further details

11
Accessories for CKX41/CKX31

IX2-SLP IX2-SL CKXN-RC


Phase contrast slider (pre-centered) Phase contrast slider (centerable) Relief Contrast condenser
Centering-free type phase slider. Centerable type phase slider. The reduced halo of the Relief Contrast
A common phase annulus for 10x, 20x and The centering unit for phase annuli is improves information of the cell's interior.
40x enables fast and easy operation for available for precise adjustment. Maintains the same shadow direction even
routine use. if the magnification is changed.

CKX-RFA CK40-CPG30 IX2-BCTP


Fluorescence illuminator Glass stage insert plate Hemacytometer holder
Provides fluorescence observation (B and Easily recognition of objectives. The mechanical stage offers excellent
G excitation) for CKX41. An insert with ø30mm opening is available inspection performance with hema-
(CKX41 only). cytometer holder or other micro plates.
As a joint development with Narishige Scientific lab, the ON3

ON3 Series micromanipulators offer high-precision and easy operation for IVF,
injection and physiological experiment.
MICROMANIPULATORS

ON3-99D (1:1)
Oil-hydraulic micromanipulator
system
The ON3-99D consists of a pair of three-
axis motorized positioners, drop handle
joystick micromanipulators and UT-D
universal joints. The ON3-99D is ideal for
ICSI application.
Two types of injectors, the IM-9B
microinjector (for sperm injection) and the
IM-9C pneumatic injector (for oocyte
holding) are available separately.
Drop handle joystick
•Fine movement range: 10mm
•Full rotation of knob: 250µm
•Minimum graduation: 2.5µm
•Joystick (for X and Y movement): 400µm max.
(movement ratio and lever tension adjustable)

Motorized positioner
•Coarse movement range: 23mm
(movement speed adjustable)

12

ONO-301D MHW-3
Drop handle joystick micromanipulator (1:1) Three axis water hydraulic drum type micromanipulator
Thanks to its symmetrical design, this micromanipulator can be attached The MHW-3 is useful for physiological experiments as well as fine
on either the right or the left side. In combination with the optional universal injection or suction. The solid design including mechanical coarse
joint (UT-D) and return mechanism (UT-R), it also provides a pipette return
function. positioner provides reliable stability and precise movement.
The optional MHW-4 single axis micromanipulator is mountable.
•Accessory: IP plate
•Fine movement range: X, Y and Z axes 10mm •Fine movement range: 2mm
•Full rotation of knob: 250µm •Coarse movement range: 30mm
•Minimum graduation: 2.5µm •Full rotation of knob: 50µm
Photo: ONM-2D, ONO-301D, UT-D and UT-R configuration
•Minimum graduation: 0.2µm
This series employs UIS2 optics to achieve unsurpassed
performance in polarized light observation. These units deliver
optimum compensation for optical aberrations to achieve images
of unprecedented sharpness. Six compensator are available to
POLARIZING MICROSCOPES allow observation and measurement at various retardation levels.
•Conoscopic/orthoscopic version and Orthoscopic version available.
•6 different kinds of compensator are available for BX51-P.
•Accessories and video/camera system of BX2 Series mountable on
BX51-P.

Illumination Transmitted light 12V100W halogen Koehler illumination


Polarizing Swing-out focusable Bertrand lens with slot for 360° rotatable
intermediate tubeanalyzer for conoscopic & orthoscopic observation (U-CPA)
Test plate 1 wavelength (1λ), 1/4 wavelength (1/4λ)
Compensators Berek, Senarmont, Brace-Koehler, quartz wedge, etc.
(6 types available)
Focusing Coarse & fine coaxial handle; full stroke: 25mm;
minimum fine adjustment: 1µm
Observation tube Trinocular (F.N. 22)
Nosepiece Detachable quadruple nosepiece with centering adjustment
function
Stage Circular rotatable stage with centering adjustment function and
attachable mechanical stage.
360° graduated in 1° increments, lockable in any position
Condenser Achromat strain-free condenser with built-in 360° rotatable
Conoscopic/ Orthoscopic version polarizer (N.A. 0.18-0.9)
orthoscopic version *Please refer to BX51-P catalog for further details

13

The CX31-P is a high-quality polarizing microscope that’s ideal for


training, with the wide-ranging functions and superior durability
required in every field of research.
POLARIZING MICROSCOPE Its excellent optical performance is matched with the versatility to
meet the demands of many different kinds of applications, from
double-refraction examination of the structure and characteristics
of transparent specimens to complex analyses of rocks, fibers,
macromolecules and new materials.

Optics Objective ACHN-P series, UPLFL-P series


Eyepiece WHN10X, WHN10X-H, CROSSWHN10X,
WHB10X3, WHB10X2-H
Observation Binocular U-BI30P, U-CBI30-2
tube Trinocular U-TR30-2, U-CTR30-2
Conoscopic Bertrand lens Incorporated, detachable, focusable
Intermediate Changeover between Engage or disengage of Bertrand lens
tube orthoscopic/conoscopic Position: [ IN
(U-PA) observation Position: _ OUT
Analyzer Incorporated, detachable, 180° rotatable,
lockable in any position, 2° increments,
minimum retardation resolution 6',
using vernier scale
Slot for compensators Tint plate (U-TP530), 1/4 wavelength
retardation plate (U-TP137) and
various compensators attachable
*Please refer to BX31-P catalog for further details
The system modularity allows users to create the application
dedicated configurations they need. Offering optical and mechanical
excellence and stability, and a wide range of modularity
RESEARCH STEREOMICROSCOPE components, the SZX Series is today's first choice in research stereo
SYSTEM microscopy.
SZX12 --------------------------------------------------------------------------------------------------------------------------
Offers a zoom ratio of 12.86:1, the SZX12 is ideal for the most
demanding applications. The SZX12 is equipped with parfocal
lenses: two objectives mounted on a revolving nosepiece allows
speedy continuous observation, covering a total magnification range
of 3.5X - 144X*. The total available magnification ranges are: 2.1X -
675X. *using 10X eyepieces and the DFPLFL0.5X PF and DFPLFL1.6X PF objectives
SZX9 -----------------------------------------------------------------------------------------------------------------------------
Cost efficiency model. Zoom ratio of 9.05:1 puts it firmly at the top
of its class. Objective lens establishes a long working distance and
contributes to consistently excellent operation. An optional aperture
diaphragm unit (SZX-AS) can be attached.
SZX12 SZX9
Optical system Galilean type zoom stereo microscope
Zoom range 0.7X-9X (zoom ratio 12.86), 0.63X-5.7X (zoom ratio 9.05),
click stop equipped (releasable) click stop equipped (releasable)
Aperture diaphragm Built-in Optional (SZX-AS)
Total mag. range 2.1X-675X 3.15X-342X
Working distance 198 (with DFPLFL 0.45X) - 198 (with DFPLFL 0.5X) -
7mm (with DFPLAPO 1XPF+ 34mm (with DFPL 2X-3)
SZX-AL20X)
Observation 1. SZX-TR30: Trinocular (F.N. 22), 30° inclination
heads 2. SZX-TBI: Binocular (F.N. 22), 5-45° variable inclination
3. SZX-BI30: Binocular (F.N. 22), 30° inclination
Illumination 1. SZX-ILLB2: 6V30W halogen
SZX12 bases hi-level transmitted light illumination base
2. SZX-ILLD2: 6V30W halogen
brightfield/darkfield transmitted light illumination base
3. SZX-ILLK: 6V30W halogen transmitted light illumination base
SZX9
Objectives DFPLFL 0.3X, DFPL 0.5X,
DFPLFL 0.45X, DFPL 0.75X,
DFPLFL 0.5XPF, DFPLAPO 1X-2,
DFPLAPO 1XPF, SZX-ACH 1X, 14
DFPLAPO 1.2XPF2, DFPL 1.5X,
DFPLFL 1.6XPF, DFPL 2X-3
SZX-AL 20X
Eyepieces 10X (F.N. 22), 15X (F.N. 16), 20X (F.N. 12.5), 30X (F.N. 7)
Focusing unit 1. SZX-FOF: Coaxial coarse and fine focus, stroke 80mm
2. SZX-FO: Coarse focus, stroke 80mm
Accessories Coaxial illuminator, beam splitter, fluorescence illuminator,
revolving nosepiece, eyepoint adjuster, large stage plate,
arrow pointer, drawing attachment, side by side discussion tube,
stage adapter, etc.
*Please refer to SZX12/SZX9 catalog for further details

SZX-RFL2 SZX-RFL3
Coaxial fluorescence illuminator Oblique light fluorescence illuminator
Coaxial reflected light fluorescence Oblique light fluorescence illuminator with
illuminator with 4-position slider. Suitable 5-position turret and 8 filter selections.
for all research observations using GFP The excitation light path and observation
and excitations with longer wavelength light path are independent, allowing
than B excitation. fluorescence observation with high image
contrast, even with UV excitation Filter
sets for CFP, RFP and GFP are available.

SZX12+SZX-RFL3
The SZX minimizes strain and fatigue while fulfilling the key mission
of Olympus microscope designers — to provide the optimal image
for any specimen. The clear, accurate performance of the high-
STEREOMICROSCOPE SYSTEM level Galilean optical system is complemented by a Distortion Free
objective lens series with maximum Numerical Aperture.
The SZX7 microscope body is manufactured using newly
developed lead-free optics, demonstrating Olympus' commitment
to protect the environment.

Optical system Galilean type zoom stereo microscope


Zoom microscope Zoom ratio values: 7:1(0.8x to 5.6)
body Lead-free
Observation tube 1. SZX-BI45: Binocular, 45° inclination
2. SZX-TBI: Binocular, 5°-45° variable inclination
3. SZX-TR30: Trinocular, 30° inclination
3. SZX-TR30PT: Trinocular, 30° inclination
All observation tube Lead-free
Interpupillary distance adjustable range: 50 to 76 mm
Objective lens DFPL0.5x-4, DFPL0.75x-4, DFPLAPO1x-4,
SZX-ACH1x, SZX-ACH1.25x, DFPL1.5x-4, DFPL2x-4
All objectives: lead-free
Eyepieces "ComfortView" WHSZ series
All eyepieces: Lead-free
*Please refer to SZX7 catalog for further details

Incorporating new improvements to the highly-regarded


Greenough optical system, the SZ61 and SZ51 successfully meet
the demand for a variety of observation and documentation
options in a genuinely compact microscope design. Clear, sharp
ZOOM STEREOMICROSCOPE image reproduction is matched by new ergonomic design
elements which maximize comfort and ease of use.
The SZ61 and SZ51 microscope bodies are manufactured using
newly developed lead-free optics, demonstrating Olympus'
commitment to protect the environment.

15
SZ61 SZ61-60 SZ61TR SZ51 SZ51-60
Optical system Greenough type zoom stereo microscope
Zoom ration 6.7:1 5:1
Working distance 110mm
Tube inclination angle 45° 60° 45° 60°
Video camera C-mount
adaptability — (0.5x built in) —
Optical component Lead-free
Auxiliary objective Mounting by screwing into the thread at the bottom of frame
(M48 thread x 0.75)
Eyepieces "ComfortView" WHSZ series
All eyepieces:Lead-free
SZ61/SZ51 SZ61TR SZ61-60/SZ51-60 *Please refer to SZ61/SZ51 catalog for further details

The SD/SF compact stereo microscope series features

SD/SF Series Greenough optics. The long 110mm working distance and
increased focal depth combine to make these microscopes
perfect for all applications from biological research to
STEREO MICROSCOPES
training/educational use.

SD SF
Optical system Greenough type stereo microscope
Magnification 1× & 3× (switchable) 2× (SF20 Series)
1× (SF10 Series)
SF
Working distance 110mm
Observation tube
45°/60° 45°/60°
angle
SD
*Please refer to SD/SF catalog for further details
Accessories for SZX/SZ
SZ2-ILST SZ2-ILA
LED illuminator stand Transmitted illumination attachment
The world's first LED stand features a Used with the SZ2-ST stand, this cost-
thin design to keep sample positions effective illumination stand provides
low and to optimize operability. bright, uniform illumination from low
Simultaneous transmitted and reflected to high magnifications. Tiltable
light are available on this stand. LED light mirror provides direct and oblique
offers both long lifetime and constant color illumination for low contrast
temperature at any intensity. specimen. Available 22W and
100W lamphouses provide
necessary power for a variety of
illumination needs.

SZ2-LGDI
Interlock dual light guide
Standard oblique semi-rigid fiber optic
light guide. The light source position SZ2-LGSF
on the rear side of the stand saves Flexible light guide
desk space.
A single fiber optic guide is fixed at the
back of the objective so as not to disturb
microscope operation.

16

SZ2-LGR
Ring light guide
Used with LGB illumination system, this
ring light guide provides bright and
SZ2-STU3 uniform images.
Table clamp stand
The versatile SZ2-STU3 can be fixed to
the side of a desk to expand the
working area. Also, this stand exhibits
operational versatility in anatomical and
electrophysiological experimentation.
• For use with SZ2-STB1 bonder arm

SZ2-STU2
Universal stand type 2
This versatile stand allows smooth
adjustment of both arm angle and
length. This stand is perfect for
photomicrography and video mounting
of large specimens.
• For use with SZ2-STS arm
DP70
DIGITAL CAMERA

The DP70 employs a 2/3 inch, 1.45 million pixel CCD, which can
be used together with the image shift mechanism to shoot images
equivalent to 12.5 million pixel resolution. By combining Olympus
digital technologies refined through the development of consumer
digital cameras, together with high-speed processing hardware,
even an image of 12.5 million pixels can be captured at high
speed, around 3 seconds, while fully maintaining image quality,
accuracy and color fidelity. High sensitivity and low noise ensure
that even images derived from relatively faint fluorescence can be
acquired clearly. The DP70 is a highly efficient and versatile digital
camera system with an abundant lineup of software.
* Please refer to DP70 catalog for further details
DP70+BX51+PC

DP30BW
HIGH SENSITIVITY
COOLED CCD CAMERA

Using its Peltier-cooled system, the DP30BW offers quiet,


vibration-free operation. Combined with the built-in shutter and
new background subtract function (noise at long exposures is
17
reduced by using this), these features enable high-quality
recording of even weak fluorescence images.

* Please refer to DP30BW catalog for further details

DP30BW+BX51

DP12
MICROSCOPE DIGITAL CAMERA

The overall design is compact, with a palm-size multi function control


unit integrating a 3.5" LCD monitor with 200,000-pixel display, and a
small footprint that makes it easy to install and lay out any necessary
auxiliary equipment. The 3.34 million-pixel and 1/1.8 inch progressive
scanning CCD system ensures highly precise digital images which
can be stored at a maximum resolution of 2048x 1536.

* Please refer to DP12 catalog for further details

DP12+BX51+PC
PM30
AUTOMATIC PHOTOMICROGRAPHIC
SYSTEM

The PM30 features the exclusive Super FL Auto mode to ensure


foolproof photomicrography for fluorescence microscopy despite
the extremely difficult parameters of this microscopy.

* Please refer to PM30/20 catalog for further details

PM20
AUTOMATIC PHOTOMICROGRAPHIC
SYSTEM

The PM20 boasts normal Auto mode and a new FL Auto mode,
which uses specially developed exposure adjustment to simplify
intricate fluorescence photomicrography.
18
* Please refer to PM30/20 catalog for further details

PM10SP
AUTOMATIC PHOTOMICROGRAPHIC
SYSTEM

The PM10SP features 1% spot measurement, 3 zones of


reciprocity failure adjustment, auto/manual/time measurement
modes and AE lock function. 35mm operation features a
MULTI.EXP. mode to accommodate multi-exposure photography.

* Please refer to PM10SP catalog for further details


The advanced UIS2 system delivers
high performance over a wider wavelength spectrum.

19

UIS2 optics inherit high expandability Flat, high transmission over wide Complete chromatic aberration
As heir to Olympus' infinity-corrected wavelength range from UV to IR compensation up to near infrared
optical system, in which the tube lens is UW multi-coatings also yields a flat, high region
built into the observation tube, UIS2 optics transmission over a wide wavelength UPLSAPO objectives completely eliminate
display no image deterioration even when range, ensuring high performance in chromatic aberration up to the near
many different optical components or research tasks using different types of infrared region, matching the ability of
equipment are inserted in the parallel light fluorochromes. Super Apochromat objectives to provide
path. This inherent expandability gives clear images without overlapping colors or
users ample freedom to construct the High transmittance
color shift. As a result, a single objective
system in a way that meets their specific UPLSAPO100XO can perform imaging from UV to IR
requirements. wavelengths.
100 (new) UPLSAPO100XO
UPLSAPO series
90 chromatic aberration compensation
80 Comparing chromatic aberration compensation levels:
UW (Ultra wideband) multi-coatings (The smaller the figure the better)
70 2
Transmittance(%)

reduces autofluorescence and


60
improves S/N ratio 50 1.5
By using carefully selected raw materials 40
for glass, and applying advanced UW 30 1
(conventional) UPLAPO100XO
Focus (µm)

multi-coatings technology, Olympus has 20 (conventional) PLAPO100XO


reduced objective autofluorescence and 10 0.5
significantly improved the S/N ratio. 0
300 400 500 600 700 800
0

(new) UPLSAPO100XO
-0.5
400 450 500 550 600 650 700 750 800 850 900
Wavelength (nm) UPLSAPO100XO
UPLAPO100XO
UIS2/UIS Series Objectives
Universal objectives

UPLSAPO series UPLFLN series UPLFL-P series


The top-performance universal Plan Super These affordable Semi-Apochromat universal These Strain-free Semi-Apochromat universal
Apochromat objective offer an unbeatable objectives deliver superb resolution, contrast objectives reduce internal strain to an absolute
solution to every kind of digital imaging need. and flatness for any microscopic technique. minimum and are best suited for polarizing and
Nomarski DIC microscopies.

Brightfield objectives

PLAPON series PLN series PLFL series


Designed for unsurpassed resolution and contrast, These cost-effective Achromat objectives ensure Despite its high magnifying power, the PLFL100X is an
these Plan Apochromat objectives keep chromatic field flatness up to F.N. 22 and are widely used easy-to-use, non-oil immersion type objective. The
aberration down to an absolute minimum. in research, educational and routine work PLFL0.5X is designed for observation of specimens up to
applications. 53mm in diameter, enabling the recording and observation
of such specimens as entire brain slices or small animals.

Objectives for special purpose

20

UPLFLN-PH series No cover objectives UAPO/340 series


The newly designed phase annuli reduce flare These no cover objectives are specially These objectives feature a highest transmission
and halo to a minimum and ensure high designed for microscopy without a cover slip of 340nm wavelength light, ensuring maximum
resolution and contrast for unstained specimens, such as for blood smear specimens. performance in fluorescence microscopy through
i.e. living cells and microorganisms. UV excitation including CA2+ photometry.

Objectives for BX51WI/BX61WI

UMPLFLW, LUMPLFLW series LUMPLFLW/IR series XLFLUOR/340 series, XLUMPLFL20XW


These objectives address the need for high These objectives are specially designed for XLFUOR/340 series objectives is designed for
transmission from the near UV to visible light. visible band near IR spectral regions. Near IR- low magnification fluorescence observation.
For ratio imaging (fura-2, 340nm transmission DIC imaging deep within thick brain sections can High N.A. long W.D. XLUMPLFL20XW objective
requirement) fluorescence and DIC observation. be observed. allows the measurement of cell membrane
electric potential.

Objectives for inverted microscopes

LUCPLFLN-PH series LUCPLFLN series LCACHN series


These objectives are exclusively designed for culture These Semi-Apochromat objectives are These Achromat phase-contrast objectives are
specimens. These objectives are exclusively dedicated for tissue culture and offer excellent designed for cell culture observations and are
designed for culture specimens. An excellent contrast and resolution in brightfield, Nomarski best suited for various clinical examinations
phase-contrast image is assured regardless of the DIC and fluorescence observations. and cell testing.
thickness and material of the vessel.
UIS2 objectives * * All UIS2 objectives and WHN eyepieces: lead-free eco-glass

Description N.A. W.D. F.N. Cover Immersion Spring Correction Iris Water proof & For upright For inverted
(mm) glass ring diaphragm oil proof function microscope microscope

UPLSAPO UPLSAPO 4X 0.16 13 26.5 — _ _


UPLSAPO 10X 0.40 3.1 26.5 0.17 _ _
UPLSAPO 20X 0.75 0.6 26.5 0.17 _ _ _
UPLSAPO 40X 0.90 0.18 26.5 0.11-0.23 _ _ _ _
UPLSAPO 60XW 1.20 0.28 26.5 0.15-0.2 Water _ _ _ _ _
UPLSAPO 60XO 1.35 0.15 26.5 0.17 Oil _ _ _ _
UPLSAPO 100XO 1.40 0.13 26.5 0.17 Oil _ _ _ _
PLAPON PLAPON 1.25X 0.04 5 26.5 — _
PLAPON 2X 0.08 6.2 26.5 — _
PLAPON 60XO 1.42 0.15 26.5 0.17 Oil _ _ _ _
PLAPON 60XOTIRFM 1.45 0.1 26.5 0.13-0.19 Oil _ _ _ _ _
UPLFLN UPLFLN 4X 0.13 17 26.5 — _ _
UPLFLN 10X 0.30 10 26.5 — _ _
UPLFLN 20X 0.50 2.1 26.5 0.17 _ _ _
UPLFLN 40X 0.75 0.51 26.5 0.17 _ _ _
UPLFLN 40XO 1.30 0.2 26.5 0.17 Oil _ _ _ _
UPLFLN 60X 0.90 0.2 26.5 0.11-0.23 _ _ _ _
UPLFLN 60XOI 1.25-0.65 0.12 26.5 0.17 Oil _ _ _ _ _
UPLFLN 100XO 1.30 0.2 26.5 0.17 Oil _ _ _ _
UPLFLN 100XOI 1.3-0.6 0.2 26.5 0.17 Oil _ _ _ _ _
UPLFLN 4XPH 0.13 0.17 26.5 — _
UPLFLN 10XPH 0.30 10 26.5 — _
UPLFLN 20XPH 0.50 2.1 26.5 0.17 _ _
UPLFLN 40XPH 0.75 0.51 26.5 0.17 _ _
UPLFLN 60XOIPH 1.25-0.65 0.2 26.5 0.17 Oil _ _ _
UPLFLN 100XOPH 1.30 0.2 26.5 0.17 Oil _ _
PLN PLN 2X 0.06 5.8 22 — _
PLN 4X 0.10 18.5 22 — _
PLN 10X 0.25 10.6 22 — _
PLN 20X 0.40 1.2 22 0.17 _ _
PLN 40X 0.65 0.6 22 0.17 _ _
21
PLN 50XOI 0.90-0.50 0.2 22 — Oil _ _ _
PLN 100XO 1.25 0.15 22 — Oil _ _
PLN-PH PLN 10XPH 0.25 10.6 22 — _
PLN 20XPH 0.40 1.2 22 0.17 _
PLN 40XPH 0.65 0.6 22 0.17 _ _
PLN 100XOPH 1.25 0.15 22 — Oil _ _
PLN & ACHN-P PLN 4XP 0.10 18.5 22 — _
ACHN 10XP 0.25 6 22 — _
ACHN 20XP 0.40 3 22 0.17 _
ACHN 40XP 0.65 0.45 22 0.17 _ _
ACHN 100XOP 1.25 0.13 22 — Oil _ _
LUCPLFLN LUCPLFLN 20X 0.45 6.6-7.8 22 0-2 _ _
LUCPLFLN 40X 0.60 2.7-4 22 0-2 _ _
LUCPLFLN 60X 0.70 1.5-2.2 22 0.1-1.3 _ _
LUCPLFLN 20XPH 0.45 6.6-7.8 22 0-2 _ _
LUCPLFLN 20XRC 0.45 6.6-7.8 22 0-2 _ _
LUCPLFLN 40XPH 0.60 3.0-4.2 22 0-2 _ _
LUCPLFLN 40XRC 0.60 3.0-4.2 22 0-2 _ _
LUCPLFLN 60XPH 0.70 1.5-2.2 22 0.1-1.3 _ _
UPLFLN-PH UPLFLN 4XPH 0.13 17 26.5 — _
UPLFLN 10XPH 0.30 10 26.5 — _
UPLFLN-PHP UPLFLN 4XPHP 0.13 16.4 22 — _
CPLFLN CPLFLN 10XPH 0.30 9.5 22 1 _
CPLFLN 10XRC 0.30 9 22 1.5 _
LCACHN LCACHN 20X PH 0.40 3.2 22 1 _
LCACHN 20X PHP 0.40 3.2 22 1 _
LCACHN 20XRC 0.40 2.8 22 1.5 _
LCACHN 40XPH 0.55 2.2 22 1 _
LCACHN 40XPHP 0.55 2.2 22 1 _
LCACHN 40XPHP 0.55 1.9 22 1.5 _
CACHN & CPLN CACHN 10XPHP 0.25 8.8 22 1 _
CPLN 10XPH 0.25 10 22 1 _
CPLN 10XRC 0.25 9.7 22 1.5 _
UIS objectives
Description N.A. W.D. F.N. Cover Immersion Spring Correction Iris Water proof & For upright For inverted
(mm) glass ring diaphragm oil proof cap microscope microscope

UPLAPO UPLAPO 10XO3 0.40 0.24 26.5 0.17 Oil _ _ _ _


UPLAPO 10XW3 0.40 0.43 26.5 0.17 Water _ _ _
UPLAPO 20XO3 0.80 0.19 26.5 — Oil _ (_) _ _
UPLAPO 40XOI3 1.00-0.50 0.12 26.5 — Oil _ _ (_) _ _
PLAPO PLAPO 40X 0.95 0.13 26.5 0.11-0.23 _ _ _ _
UPLFL-P UPLFL 4XP 0.13 13 26.5 — _
UPLFL 10XP 0.30 3.1 26.5 — _
UPLFL 20XP 0.50 1.6 26.5 0.17 _ _
UPLFL 40XP 0.75 0.51 26.5 0.17 _ _
UPLFL 100XO3P 1.30 0.1 26.5 0.17 Oil _ _
PLFL PLFL 0.5X 0.02 7 26.5 —
PLFL 100X 0.95 0.2 26.5 0.14-0.2 _ _ _
UAPO UAPO 10X/340
UAPO 20X3/340 0.75 0.55 22 0.17 _ _ _
UAPO 40X3/340 0.90 0.2 22 0.11-0.23 _ _ _ _
UAPO 40XOI3/340 1.35-0.65 0.1 22 0.17 Oil _ _ _ _
UAPO 20XW3/340 0.70 0.4 22 0.17 Water _ _ _
UAPO 40XW3/340 1.15 0.25 22 0.13-0.25 Water _ _ _ _
APO APO 100XOHR 1.65 0.1 22 0.15 Oil _ _ _
Low magnification XLFLUOR 2X/340 0.14 21** 22 0-5 (Water)
fluorescence XLFLUOR 4X/340 0.28 29.5** 22 0-5 (Water)
Super high N.A. XLUMPLFL 20XW 0.95 2 22 Water
No cover MPLAPO 50X 0.95 0.3 0 _ _
objective MPLAPO 60X 0.90 0.4 0 _ _
MPLAPO 100XO 1.40 0.1 0 Oil _ _
UMPLFL 40X 0.75 0.63 0 _ _
UMPLFL 50X 0.80 0.66 0 _ _
UMPLFL 100X 0.95 0.31 0 _ _
UMPLFL 10XW 0.30 3.3 26.5 — Water _
UMPLFL 20XW 0.50 3.3 26.5 — Water _
UMPLFL 40XW 0.80 3.3 26.5 0 Water _
22
UMPLFL 60XW 0.90 2 26.5 0 Water _
LUMPLFL 40XW/IR2 0.80 3.3 26.5 0 Water _
LUMPLFL 60W/IR2 0.90 2 26.5 0 Water _
LUMPLFL 100XW
LSM objective PLAPO 40XWLSM 0.90 0.16 22 0.17 Water _ _ _ _
PLAPO 60XWLSM 1.00 0.15 22 0.17 Water _ _ _ _
PLAPO 40XOLSM 1.10 0.13 22 0.17 Oil _ _ _ _
** Include 5mm water (_): oil proof cap applicable
Patch clamping Time Lapse

TIRFM Digital imaging

Specifications are subject to change without any obligation on the part of the manufacturer.

This catalog is printed by enviromentally-friendly


waterless printing system with soy ink. 

Printed in Japan M05E

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