SYLLABUS FOR B.

TECH PROGRAM IN BIOTECHNOLOGY

SYLLABUS OF B.TECH PROGRAM IN BIOTECHNOLOGY

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
MAC331 MATHEMATICS- PCR 3 1 0 4 4
III
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
Basic knowledge of topics CT+EA
included in MAC01 &
MAC02.
Course  CO1: Acquire the idea about mathematical formulations of phenomena in
Outcomes physics and engineering.
 CO2: To understand the common numerical methods to obtain the approximate
solutions for the intractable mathematical problems.
 CO3: To understand the basics of complex analysis and its role in modern
mathematics and applied contexts.
 CO4: To understand the optimization methods and algorithms developed for
solving various types of optimization problems.
Topics Partial Differential Equations (PDE): Formation of PDEs; Lagrange method for
Covered solution of first order quasilinear PDE; Charpit method for first order nonlinear
PDE; Homogenous and Nonhomogeneous linear PDE with constant coefficients:
Complimentary Function, Particular integral; Classification of second order linear
PDE and canonical forms; Initial & Boundary Value Problems involving one
dimensional wave equation, one dimensional heat equation and two dimensional
Laplace equation. [14]

Numerical Methods: Significant digits, Errors; Difference operators; Newton's

Forward, Backward and Lagrange’s interpolation formulae; Numerical solutions of
nonlinear algebraic/transcendental equations by Bisection and Newton-Raphson
methods; Trapezoidal and Simpson’s 1/3 rule for numerical integration; Euler’s
method and modified Eular's methods for solving first order differential equations.
[14]

Complex Analysis: Functions of complex variable, Limit, Continuity and

Derivative; Analytic function; Harmonic function; Conformal transformation and
Bilinear transformation; Complex integration; Cauchy’s integral theorem;
Cauchy’s integral formula; Taylor’s theorem, Laurent’s theorem (Statement only);
Singular points and residues; Cauchy’s residue theorem.
[17]

Optimization:
Mathematical Preliminaries: Hyperplanes and Linear Varieties; Convex Sets,
Polytopes and Polyhedra.
[2]

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Linear Programming Problem (LPP): Introduction; Formulation of linear

programming problem (LPP); Graphical method for its solution; Standard form of
LPP; Basic feasible solutions; Simplex Method for solving LPP.
[9]
Text Books, Text book:
and/or 1. An Elementary Course in Partial Differential Equations-T. Amarnath
reference 2. Numerical Methods for scientific & Engineering Computation- M.K.Jain,
material S.R.K. Iyengar & R.K.Jain.
3. Foundations of Complex Analysis- S. Ponnuswami
4. Operations Research Principles and Practices- Ravindran, Phillips, Solberg
5. Advanced Engineering Mathematics- E. Kreyszig

Reference Book:
1. Complex Analysis-L. V. Ahfors
2. Elements of partial differential equations- I. N. Sneddon
3. Operations Research- H. A. Taha

CO-PO mapping:

Course Code: MAC331 Course Title: MATHEMATICS-III

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 2 2 2 2 3

CO2 1 2 1 1 3 2 1 3

CO3 3 2 1 2 2 3

CO4 3 3 3 2 1 2 1 2 3

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
CHC331 PROCESS PEL 3 0 0 3 3
CALCULATIONS
AND
THERMODYNAMICS

Mathematics I and Mathematics II Course Assessment methods (Continuous (CT) and end
assessment (EA))
CT+EA

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course  CO1: To develop the concept of dimension and unit conversion to check
Outcomes dimensional consistency of balanced equation
 CO2: Learn basic laws about the behavior of gases, liquids and solids and
some basic mathematical tools.
 CO3: To Establish mathematical methodologies for the computation of
material balances and energy balances with and without chemical reaction
 CO4: To apply knowledge of the laws of thermodynamics to solve physical
and chemical problems encountered in chemical and biochemical industries.
 CO5: To analyze and interpret data, to identify, formulate, and solve
engineering problems.
Topics Module - I (10 hrs)
Covered
 Significance of Units and Dimensions: Conversion of Equations, Systems of
Units, Dimensional Homogeneity and Dimensionless Quantities, Buckingham
Pi-theorem for Dimensional Analysis Mathematical Requisites: Use of log-log
and semi-log graph paper, Triangular Diagram.
 Introduction to Chemical Engineering Calculations: Basis, Mole Fraction and
Mole Percent, Mass Fraction and Mass Percent, Concentration of different
forms, Conversion from one form to another.
 Ideal gas laws and its significance, Molar concept, Concept of partial pressure
& partial volume, Dalton’s law and Amagat’s law and Numerical problems on
their applications.
 Fundamental concept of vapor pressure & boiling point, Clausius-Clapeyron
equation, Antoine equation and numerical problems on their applications.

 Ideal & non-ideal solutions, Raoult’s law, Henry’s law and their applications
in numerical problems.
Module – II (10 hrs)
 Material Balances with and without chemical reaction: Material balances in
crystallizers, gas - liquid absorbers, evaporators, distillation plant. Systems
with recycle,drying, extraction.
 Energy Balance: Enthalpy calculation for systems without Chemical Reaction,
Estimation of Heat Capacities of solids, liquids and gases. Heat of fusion and
vaporization
 Enthalpy calculation for systems with Chemical Reaction, Thermo-chemistry,
Calculations of heat of reaction, heat of combustions, heat of formation and
heat of neutralization, Effect of Temperature and Pressure on Heat of
Reaction, Hess’s Law, Adiabatic Flame Temperature, Theoretical Flame
Temperature.
Module – III (10hrs)
 Scope of thermodynamics, Terminology and fundamental concepts.
Microscopic and macroscopic view. State and path functions, thermodynamics
processes, Zeroth and First law of thermodynamics: Applications of first law
to close and open system.Limitations of first law, Heat pump, heat engine,
Second law of thermodynamics: Reversibility and irreversibility, Carnot cycle,
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

concept and estimation of entropy, third law of thermodynamics,

Clausiusinequality,Gibb’s and Helmholtz free energy.
Module – IV (10 hrs)
 PVT behavior of pure substance, Equations of state for ideal and real gases,
cubic andvirial equation of state, problems, Compressibility
factor,thermodynamic properties of pure substances.
 Refrigeration of gases: Refrigerator, Co-efficient of performance, capacity of
refrigerator, Vapour compressioncycle, Choice of refrigerants.
Text Books, 1. Unit Operations–Chemical Process Principles – Part-I - Haugen,
and/or Wartson&Ragatz (CBS)
reference
material 2. Basic Principles and Calculations in Chemical Engineering – Himmelblau
((Prentice Hall of India)
3. Stoichiometry, Bhatt and Vora, Tata McGraw Hill Companies.
4. Chemical Engineering Thermodynamics – J. M. Smith & H. C. Van Ness and
M. M. Abbott (Tata McGraw Hill)
5. Chemical & Engineering Thermodynamics – S. I. Sandler (Wiley)

CO-PO MAPPING:
Course Code: CHC331 Course Title: PROCESS CALCULATIONS &
THERMODYNAMICS

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO1 PO1 PO12
0 1
CO 1 3 3 2 1 1 1 3 1 1 3

CO 2 3 3 2 1 1 1 3 1 1 3
CO 3 3 3 3 1 1 1 3 1 3 3
CO 4 3 3 3 2 1 1 2 3 1 3 3

Department of Biotechnology

Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) /
Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTC CELL PCR 3 1 0 4 4

301 BIOLOGY

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

AND
GENETICS

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))

CT+EA

Course CO1: To understand the basic organization of cells and organisms and the tools
Outcomes needed to study them

CO2: To understand the basic processes of the cell machinery, cell-cell interaction
and the eukaryotic cell cycle.

CO3: To apply the knowledge of cell process regulation and cell cycle in
understanding the use of a cell as a biological tool for manufacturing biomolecules.

CO4: To learn the fundamentals of Genetics and its applications.

CO5: To solve problems associated with genetic diseases and their transmission from
one generation to the next

Topics Classical Genetics: Mendelian inheritance; Euploidy and aneuploidy (4)

Covered
Genetic interactions (2)

Molecular Genetics-Split and Overlapping genes; Transposons & Retrotransposons;

Mutation (6)

DNA Repair and human diseases (4)

Recombination (2)

Internal Organization of the cell: Cells as experimental models, Cells and cellular
organelles, Tools of cell biology- Microscopy and cell Architecture, Purification of
cells, Membrane structure, Membrane Transport of small molecules and electrical
properties of membranes (8)

Cytoskeleton and cell movement: Structure and organization of actin filaments,

Actin myosin and cell movement, intermediate filaments, microtubules, microtubule
motors and movements, cell-cell interactions (6)

Cell signalling

Signaling molecules and their receptors, function of cell surface receptors, pathways
of intracellular signal transduction, signal transduction and the cytoskeleton,
signalling in development and differentiation (6)

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Cell cycle and cancer

Eukaryotic cell cycle, meiosis and fertilization, stem cells, Development and causes of
cancer, oncogenes, tumor suppressor genes (4)

Text  Molecular Biology of Cell by Albert et.al. John Wiley & Sons
Books,  The Cell by Cooper. ASM Press
and/or  Cell and Molecular Biology by Karp. John Wiley & Sons
reference  M.W.Strickberger: Genetics, Pearson.
material  In Introduction to genetic analysis, Griffiths, Miller, Suzuki, Lewontin and
Gelbart, Freeman and Company.
 Brown, T.A., Genetics a Molecular Approach, 4th Ed. Chapman and Hall,
1992
 Stratchan& Read: Human Molecular Genetics
 David Freifelder: Microbial Genetics, Jones and Bartlett Publisher Inc. 1987

CO-PO MAPPING:
Course Code: Course Title: CELL BIOLOGY & GENETICS
BTC301

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 2

CO2 2 2

CO3 2 2 3 2 1 3 2

CO4 1 2 2 1

CO5 2 2 2

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Electives (L) (T) (P) Hours

(PEL)

BTC MICROBIOLOGY PCR 3 1 0 4 4

302 AND
BIOPROCESS
TECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

BTC01 (LIFE SCIENCE) CT+EA

Course CO1: To develop knowledge on different types of microorganisms including viruses

Outcomes and microscopy for the visualization of microorganisms, their characteristic features
as well as internal and external structures and their functions.
CO2: To impart an understanding on microbial classification and taxonomy,
microbial community and interactions, microbial nutrition, nutritional types, growth
media, growthin different systems, and control of microorganisms using various
physical and chemical treatments including antimicrobial drugs.
CO3: To develop knowledge on microbial metabolism, energy transduction
mechanisms, and microbial genetics

CO4: To acquire experimental know how of microbial production of various

industrial products such as alcohol, antibiotics, amino acids, vitamins
exopolysaccharides, enzymes, etc. from industrial strains.
CO5: To illustrate the upstream and downstream processing for product recovery
and purification.

Topics PART A: Microbiology

Covered
Introduction to microbiology: History and scope of microbiology, major
contribution and events in microbiology, different types of microorganisms –
characteristic features, microbes and diseases, microbes in human welfare.[2]
Microbial structures: Different types of microscopy, preparation and staining of
specimens, microbial shape, size, arrangements, overview of procaryotic and
eucaryotic cell – internal and external structures, cytoplasmic matrix, nucleoid,
plasmids, ribosomes, flagella, pilli, fimbrie, spores, bacterial and archaebacterial cell
walls and cell membranes, Viruses – types, structures, multiplications [4]
Microbial classification and taxonomy: Domains of life, classification, taxonomic
ranks, techniques for determining microbial taxonomy and phylogeny, prokaryotic
phylogeny and diversity, microbial community and interactions – Mutualism,
Cooperation, Commensalism, Predation, Parasitism, Amenalism, Competition.
Normal microbiota of human body. [3]
Microbial nutrition, growth and control: Common nutrient requirements,
nutritional types, uptake of nutrients by cell, culture media, pure culture, microbial
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

growth – batch culture and continuous culture, growth curve, measurement of

growth, influence of environmental factors on growth, control of microorganisms by
physical and chemical agents, Antimicrobial drugs – general characteristics, narrow-
spectrum and broad-spectrum drugs, inhibitors of cell wall synthesis, nucleic acid
synthesis and protein synthesis, metabolic antagonists, Drug resistance.
[5]
Microbial metabolism: Energy release and conservation, chemoorganotrophic
fueling processes, aerobic respiration, glycolysis, TCA cycle, electron transport and
oxidative phosphorylation, anaerobic respiration - nitrate and sulphate reduction,
fermentations, chemolithotrophy, phototrophy [3]
Microbial genetics: Conjugation, Transduction, Transformation [4]

PART B: BIOPROCESS Technology

A) Introduction to Fermentation Technology: Microbial Culture systems; Media for
Industrial fermentations; Media Optimization; Sterilization of Industrial Media;
The development of Inoculum for Industrial fermentations; Starter Cultures;
Downstream Processing and fermentation economics.
………………………….. [4]
B) Commercial Strain Development & Microbial Processes: Sources of industrial
cultures and maintenance. Alcoholic fermentation: Production of Industrial
Alcohol – Fermentation mechanism. Recent developments, brewing and
malting, manufacture of wine and other distilled liquors. Cellular control
regulating production of microbial metabolites – Primary and Secondary
metabolite – Induced mutation technique – Analogue resistant mutant –
Catabolic derepressed mutants – Genetically engineered strain – Protoplast
fusion technique. Basic idea on fermentation process, submerged, stationary,
solid and semi-solid – with their merits and demerits. [5]
C) Microbial production of nucleosides and nucleotides: i) Introduction ii)
Classification of methods for production of 5’ IMP and 5’GMP iii) Production
of 5’IMP and 5’GMP by fermentation.
………………………………………………………[3]
D) Microbial production of Vitamins: 1) Vitamin B12 - Organisms used,
production method- process, recovery and assay. 2) Vitamin C - Organisms
used, production method, process, recovery and assay.
[3]
E) Lectures Microbial Production of Antibiotics : Organism used, production
process and recovery of- 1) Bacitracin & 2) Chloramphenicol
[2]
F) Lectures Microbial Production of acids, viz., citric, lactic, Acetic acid, vinegar
and gluconic acid. Mechanism of each fermentation, their uses. its spoilage and
prevention [2]
G) Production of Amino acids (Lysine and glutamic acid) and Antibiotics
(Pencillin, Streptomycin and Tetracyclines) and its new Developments
……………[2]

Text Books, Text Books:

and/or Prescott, Harley and Klein’s Microbiology – McGraw Hill
reference Microbiology by Pelczar, Chan and Krieg, Tata Mc Graw Hill
L.E. Casida. Jr, Industrial Microbiology, New Age International Publisher
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

material W. Crueger, Annelise Crueger, Biotechnology: A Textbook of Industrial

Microbiology, Pnima Publishing Corporation
Fermentation microbiology and biotechnology. Ed. E.M.T. El-Mansi , C.F.A.
Bryce, B. Dahhou, S. Sanchez, A.L. Demain, A.R. Allman. 3rd ed. Taylor and
Francis.
Reference books:
Microbiology by Tortora, Funke and Case
Brock Biology of Microorganisms
General Microbiology by Hans G Schlegel, Cambridge
Atkinson. B and Marituna. F, Biochemical Engineering and Biotechnology
Handbok, The Nature Press, Macmillan Publ.Ltd.4
James E Bailey, David F., Ollis, Biochemical engineering fundamentals, second
edition. McGraw Hill

CO-PO MAPPING:
Course Code: BTC302 Course Title: MICROBIOLOGY & BIOPROCESS
TECHNOLOGY

COs PO1 PO2 PO PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
3

CO1 1 2 2 2 1 - - - - - - 3

CO2 2 2 1 2 2 2 2 1 - - 1 2

CO3 2 2 2 2 2 1 2 2 2 1 - 3

CO4 3 2 2 2 2 2 2 1 2 - 1 2

CO5 3 3 2 2 2 2 2 2 2 1 2 2

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTC303 BIOCHEMISTRY PCR 3 0 0 3 3

AND ENZYME
TECHNOLOGY

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To understand the principles of bioenergetics and to correlate them with the
Outcomes metabolic pathway.
CO2: To impart an understanding on the fates of macromolecules during
metabolism.
CO3: To provide an understanding on the importance and synthesis of energy
currency molecule, ATP.
CO4: To interpret the regulation in the metabolic pathway and to study the role of
hormones in the metabolic pathway.
CO 5: To understand mechanism and kinetics of enzyme action and their
regulation for application of enzymes in living system and for industrial purpose.
Topics Module 1 (3+2)5
Covered
Biomolecules, Vitamins

Principles of Bioenergetics

Module 2

Carbohydrate and its metabolism 5

Carbohydrate Biosynthesis - Gluconeogenesis, Biosynthesis of glycogen, starch,

Sucrose , Photosynthetic Carbohydrate Synthesis,

Glycolysis and catabolism of hexoses - Glycolysis, pentose phosphate pathway

of glucose oxidation, Citric acid cycle, regulation of citric acid cycle, glyoxylate
cycle . Role of hormones in metabolism

Oxidative Phosphorylation and Photo Phosphorylation - Oxidative

Phosphorylation, Regulation of Oxidative Phosphorylation, Photosynthesis

Module 3 3

Lipid and its metabolism

Oxidation of Fatty acids - Transport of fatty acid, beta-oxidation, Ketone bodies

Lipid Biosynthesis - Biosynthesis of fatty acids

Module 4 3

Protein and its metabolism

Amino acid oxidation and production of Urea - Metabolic fates of amino

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

groups, Nitrogen excretion and the urea cycle, Pathways of amino acid degradation

Nitrogen metabolism, Biosynthesis of amino acids,

Module 5 2

Nucleic acid and its metabolism

Biosynthesis and degradation of Nucleotides

Module 6 12

Enzyme Technology and Vitamins

Enzymes:Nomenclature of enzymes, Enzyme kinetics, Mechanism of enzymatic,

Catalysis, Active site, Activators and inhibitors, Coenzymes, Isoenzymes,
Michaelis-Menten equation, Km and Vmax value, Regulation of enzyme activity
(single-substrate and multi-substrate reactions). Vitamin’s as coenzyme

Production of enzymes and immobilisation : Production of industrial enzymes

such as proteases, amylases, lipases, cellulases, whole cell biocatalysis. Enzyme
immobilization: Methods of immobilization of enzymes-physical & chemical
techniques, Kinetics of immobilized enzyme, Effect of external mass transfer &
intra-particle diffusion, limitation & applications of immobilized enzymes,
Bioreactors using immobilized enzyme. Engineering of Enzymes

Application of enzyme in leather industry, detergent industry, dairy industry;

Lignocellulose degrading enzymes.

Text Books, Text

and/or 1. Biochemistry by LubertStryer. W. H. Freeman & Company, NY
reference
2. Biochemistry by Lehninger. McMillan publishers
material

Reference:

1. Biochemistry, Voet&Voet
2. Fundamental of Enzymology by Price and Stevens (2002): Oxford
University Press
3. Enzyme technology by Chaplin and Bucke. Cambridge Univerity Press

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING:

Course Code: BTC303 Course Title: BIOCHEMISTRY AND ENZYME TECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 2 3 3 2 2 1 1 1 3

CO2 3 3 3 3 3 2 2 3 1 1 1 3

CO3 3 3 3 3 2 3 1 1 1 1 1 3

CO4 3 3 2 3 3 3 1 1 1 1 1 3

CO5 3 3 3 3 3 3 3 3 3 3 1 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTS351 MICROBIOLOGY PEL 0 0 3 3 1.5

LABORATORY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To learn and become familiar with types of culture media, preparations of
Outcomes culture media, sterilization procedures, types of equipments.

CO2: To understand the concept of sterility, working principles and applications of

instruments: autoclaving, laminar air flow hood etc.

CO3:To learn about the isolation and maintenance process of bacterial cultures.

CO4: To apply the understanding of the cultural and morphological characteristics

of microorganisms grown in pure culture.Applications in Antimicrobial effect and

CO5: To interpret microbial growth phases its kinetics specific growth rate.to
determine the effects of chemicals on bacteria and to understand the quality of

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

water.

Topics Microbial culture media preperation:

Covered
Basic concepts of nutrition materials in media, classes of culture media, how to
prepare growth media.

The control of microbial growth :

To study the methods of sterilization: autoclaving, laminar air flow hood,

irradiation, filtrations, chemical and gas.

Isolation of microorganisms from an environment of choice :

To demonstrate the ubiquity and diversity of microbes in the environment, samples

from immediate areas of the environment will be obtained and cultured and
dilution methods.

Isolation and Maintenance of pure cultures :

To study the different techniques of isolation and maintenance of pure cultures:

subculturing, streak plate method, pour plate method, spread plate method.

Bacterial morphology and staining :

To study the physical properties and differentiation of microorganisms with the

help of different staining procedures: differential and structural staining.
Techniques of Gram staining, endospores staining, microscopic study.

Estimation of coliform bacteria:

To study the estimation of coliform bacteria in water by MPN (most probable

number) test.

Study of bacterial growth:

To study the growth pattern of bacteria, specific growth rate calculation, different
growth phases of bacteria.

Antimicrobial activity study:

To determine the antibiotic susceptibility via sensitivity disk methods, calculation

of zone of inhibition.

Text Books, Textbook :

and/or 1. Benson HJ. 2002. Microbiological applications: a laboratory manual in
reference general microbiology: McGraw-Hill New York, NY.
2. Harley JP. 2004. Laboratory exercises in microbiology: McGraw-Hill
material
Science/Engineering/Math

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Reference books:
1. Brown AE. 2009. Benson's Microbiological Applications: Laboratory
Manual in General Microbiology, Short Version: McGraw Hill
2. Madigan MT, Martinko JM, Dunlap PV, Clark DP. 2012. Brock biology of
microorganisms: Pearson/Benjamin Cummings.
3. Pollack RA. 2004. Laboratory exercises in microbiology, 3e. Recherche 67:
02

CO-PO MAPPING:

Course Code: BTS351 Course Title: MICROBIOLOGY LABORATORY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 1 1 1
CO2 2 1
CO3 2 1 1
CO4 2 1 1
CO5 1 2 2 2

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTS352 BIOCHEMISTRY PCR 0 3 3 1.5

LABOARTORY

Pre-requisites BTC303

Course CO1: To design , analyze and solve problems and learn to plot graph and interpret
Outcomes data

CO2: To develop skills to perform experiments and have hands on training.

CO3: To apply the results and data to solve problems in daily activities and
industry.

Topics 1. To prepare Tris-HCl Buffer with a specific pH (eg. pH 8.8)

Covered 2. Qualitative and quantitative estimation of carbohydrates
3. Qualitative and quantitative estimation of aminoacids and determination of
the unknown concentration of protein concentration by plotting a standard
curve of BSA using Bradford reagent
4. Ammonium sulphate precipitation and dialysis for a protein
5. Separation and Identification of Amino acids by Paper Chromatography and
Thin Layer Chromatography
6. Analysis of Protein purity and determination of molecular weight of pure
protein by SDS PAGE and Coomassie Brilliant blue staining of proteins on
SDS gel
7. Extraction of Enzyme Tyrosinase from commercially available mushrooms
and Assay of Enzyme Tyrosinase with determination of specific activity of
Enzyme Tyrosinase
8. Effect of substrate concentration on the activity of Enzyme Tyrosinase and
determination of MichelesMenton parameters of Enzyme Tyrosinase
9. Effect of inhibitor concentration on the activity of Enzyme Tyrosinase

Text Books, Text Books:

and/or
reference Practical Biochemistry by David T Plummer
material Reference Books:

Biochemistry by Voet and Voet

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING:
Course Code: BTS352 Course Title: BIOCHEMISTRY LABORATORY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 3 3 2 3 3 3 3 3 3 3 2 3
CO2 3 3 2 3 2 3 3 3 3 3 2 3
CO3 3 3 2 3 3 3 3 3 3 3 2 3

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTC401 MOLECULAR PCR 3 1 0 4 4
BIOLOGY AND
rDNA
TECHNOLOGY
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
BTC01 Life Science CT+EA
BTC301 Cell Biology and
Genetics
BTC303 Biochemistry and
Enzyme Technology
Course CO1: Students will acquire basic understanding of molecular biology topics:
Outcomes nucleic acid structure and chemistry; organization of genome in chromosomes;
regulation of replication, transcription, translation and DNA repair.
CO2: Students will acquire knowledge of recombinant DNA techniques on: nucleic
acid amplification and gene cloning; manipulation of DNA sequences; preparation
and screening of nucleic acid libraries; gene silencing; analysis of variations in
genome sequence.
CO3: Students will be proficient in applying basic understanding of molecular
biology topics in analyzing and solving problems related to recombinant DNA
technology.
CO4: Students will be able to design strategies to solve problems related to
recombinant DNA technology.
Topics 1. Nucleic acid structure: Nucleotides and nucleic acids, DNA structure, different
Covered forms of DNA, unusual DNA structure, different types of RNA, RNA
structure. [3]
2. Nucleic acid chemistry: Denaturation and renaturation, hybridization,
nonenzymatic transformation (Mutation) – spontaneous and induced, point
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

mutation - transition, transversion, mutation involving more than one base

pairs, insertion, deletion, frame shift mutation, forward and back mutation, null
mutation, Loss-of-function and gain-of-function mutation, silent mutation,
DNA sequencing. [4]
3. Chromosome organization: Chromosomal elements – genes and intergenic
regions, regulatory sequences; DNA supercoiling, linking number,
Chromosome structure: Histones, Non-histones, Nucleosome, Chromatin.
Chromosome structure in prokaryotes & eukaryotes. [4]
4. DNA replication and repair: Central dogma, DNA replication in prokaryots
and eukaryots – set of fundamental rules, DNA polymerases, proteins and
enzymes involved in replication, process, accuracy. [4]
5. Transcription and post-transcriptional processing: DNA-dependent RNA
synthesis in prokaryotes and eukaryotes, RNA polymerases, transcription
process, termination, selective inhibition, RNA processing – capping, splicing
of introns, differential RNA processing; RNA-dependent synthesis of RNA
and DNA. [4]
6. Protein synthesis – translation: Genetic code, ribosome, transfer RNA, protein
biosynthesis stages – attachment of amino acid to specific tRNA, initiation,
elongation, termination, folding and processing; inhibition of protein synthesis.
[4]
7. DNA repair: DNA repair – multiple repair systems. [3]
8. Regulation of gene expression: Regulation of gene expression in bacteria -
operon concept; Regulation of gene expression in eukaryotes, hormonal
control of gene expression in eukaryotes. [3]
9. Introduction to recombinant DNA and Gene Cloning Tools of recombinant
DNA: Vectors; plasmid, bacteriophage viral vectors, cosmids, yeast artificial
chromosome. Expression vectors, and selection of suitable Host. [5]
10. Restriction endonucleases and other enzymes use and mechanism of action and
analysis, Genomic DNA and cDNA library preparation. Strategies for
engineered vectors use and regulation for enhanced gene expression and
purification. [5]
11. Screening and selection of clone with desired gene and protein of interest:
Colony and plaque hybridization. antibody based assay, Protein activity.
Application of gene cloning and DNA Analysis. [3]
12. Molecular probes: Preparation of molecular probes DNA probes, RNA
probes, radioactive labeling, Non-radioactive labeling, use of molecular probes
in DNA fingerprinting. Southern blotting, Northern blotting, Western blotting,
In-situ hybridization. [4]
13. MOLECULAR TECHNIQES: Polymerase chain reaction, different types and
their use. Antisense RNA technology, Site directed mutagenesis, Use of RFLP,
SNP and Microarray. [4]
Text Books, Text Books:
and/or 1. Gene IX by B. Lewin, Pearson
reference 2. Molecular biology of the cell by Alberts et. al., Garland science
material Reference Books
1. Molecular Biology of the Gene, 7th edition 2013. Watson et. al.
Published by Pearson.
2. Cell and molecular Biology, Concepts and experiments Gerald Karp, John
17
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Wiley and Sons.

3. The Cell - A molecular approach, GM Cooper ASM Press
4. Genomes, T. A. Brown, John Wiley and Sons PTE Ltd

CO-PO mapping:
Course Code: BTC401 Course Title: MOLECULAR BIOLOGY AND rDNA
TECHNOLOGY

COs PO PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
1

CO1 2 1 1 1

CO2 2 1 1 1

CO3 1 2 2 2 1

CO4 1 2 2 1 2 1

Department of Chemical Engineering

Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
CHC431 UNIT PCR 3 1 0 4 4
OPERATIONS
OF CHEMICAL
ENGINEERING I

Mathematics, Unit Operations Course Assessment methods (Continuous (CT) and end
assessment (EA))
CT+EA
Course  CA1:To Understand fundamentals of fluid dynamics and mechanics
Outcomes  CA2:Understanding the fundamentals of heat transfer operations
 CA3:To learn design of heat transfer equipment and calculations
 CA4:To develop knowledge of different mechanical operations and their
applications
 CA5:To solve related problems of different difficulty levels through tutorials
Topics Module - I (14 hrs)
Covered
Fundamental Concepts: Definition of Fluid, Terminologies of fluid flow, velocity –

18
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

local, average, maximum, flow rate – mass, volumetric, velocity field; flow
visualization – streamline, path line, streak line, viscosity; Newtonian fluid; Non-
Newtonian fluid; Reynold’s number—its significance, laminar, transition and
turbulent flows.
Fluid Statics: Basic equation of fluid statics; pressure variation in a static field;
pressure measuring devices– manometer, U-tube, inclined tube. Introduction to
rotational and irrotational flow. Introduction; flow of incompressible fluid in
circular pipe; laminar flow for Newtonian fluid; Hagen-Poiseullie equation;
introduction to turbulent flow in a pipe-Prandtl mixing length; energy consideration
in pipe flow, relation between average and maximum velocity, Bernoulli’s
equation–kinetic energy correction factor.
Fluid moving machines: Introduction; Basic classification of pumps: Mechanical
pump: Centrifugal pumps- cavitation, NPSH, Positive displacement pumps (rotary,
piston, plunger, diaphragm pumps); Peristaltic pump; Pump specification; Basic
characteristics curves for centrifugal pumps
Module – II (14 hrs)
Basic modes of heat transfer; Heat transfer by conduction: One dimensional steady
state heat conduction, Fourier’s Law, Thermal conductivity, Compound resistance
in series; Steady state heat transfer analysis through extended surface; Unsteady
state heat conduction with and without heat generation, Concept of thermal
diffusivity; Concept of heat transfer coefficient in convective-conductive system,
Critical thickness of insulation.
Heat transfer by convection: Convection heat transfer mechanism; Forced
convection in systems of simple geometrics (plate, cylinder etc.), Thermal
boundary layer; Co-relation for heat transfer coefficient: internal flow & external
flow, Momentum & heat transfer analogies.
Evaporation: Classification; Capacity, Steam economy; Boiling point elevation
(Duhring rule); Material and energy balance of single effect evaporator;
Introduction to multiple effect evaporator: Forward feed, Backward feed, Mixed
feed, Parallel feed
Module – III (12 hrs)
Particulate solids: Characterization of solid particles, particle shape, particle size,
mixed particle sizes and size analysis, specific surface of mixture, average particle
size.
Screen analysis: Type of screens, ideal screen, real screen, screen effective ness,
differential and cumulative analysis, screen capacity.Screening equipment:
stationary screens and grizzlies, gyrating screens, vibrating screens and other
industrial screens like trammels etc.
Comminution of solids (Size Reduction): Factors affecting
comminution,comminution laws: Kick’s law, Rittinger’s law and Bond’s law and
theirlimitations. Crushing efficiency & power consumption.
Text Books, 1. Process Heat Transfer: D. Q. Kern, MGH
and/or
reference 2. Heat Transfer Principles and Application, B. K. Dutta, PHI.
material 3. Units Operations of Chemical Engineering: McCabe & Smith and Harriot, MGH
19
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

4. Coulson, J.M., Richardson, J.F., “Chemical Engineering”, Volume 2, Third

Edition, Pergamon Press, 1977

CO-PO MAPPING:

Course Code: CHC431 Course Title: UNIT OPERATIONS OF CHEMICAL

ENGINEERING I

PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO 10 PO 11 PO 12

CO 1 1 3 3 3 2 1 1 0 3 3 1 3

CO 2 1 3 3 3 2 1 1 0 3 3 1 3

CO 3 1 3 3 3 2 1 1 0 3 3 1 2

CO 4 3 3 3 3 2 1 1 0 3 3 1 3

CO5 1 2 2 3 2 1 1 0 3 3 1 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR)
/ Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTC IMMUNOLOGY PCR 3 1 0 4 4

402

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))

CT+EA

Course CO1: To understand the role of the components of the immune system and its
Outcomes classification

CO2: To understand the role of the immune cells and their immunological response
in the context of human diseases including infectious diseases, autoimmunity, and
cancer.

CO3: To learn the fundamentals and principles of immunological techniques and

their application.

20
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO4: To understand methods of generations of Polyclonal and Monoclonal Antibody

and the use of custom made genetically engineered antibodies.

CO5: To solve problems associated with drugs and their toxic response based on the
knowledge of immunological response.

Topics Immunology- fundamental concepts and anatomy of the immune system

Covered
Components of innate and acquired immunity; Phagocytosis; Complement and
Inflammatory responses; Haematopoesis; Organs and cells of the immune system-
primary and secondary lymphoid organs; Lymphatic system; Lymphocyte
circulation; Lymphocyte homing (6)

Immune responses generated by B and T lymphocytes

Immunoglobulins-basic structure, classes & subclasses of immunoglobulins,

antigenic determinants; (2)

Multigene organization of immunoglobulin genes; B-cell receptor; Immunoglobulin

superfamily (3)

Kinetics of Active and Passive Immunity, Basis of self –non-self discrimination; (4)

B cell maturation, activation and differentiation; T-cell maturation, activation and

differentiation and T-cell receptors; Functional T Cell Subsets; Cell-mediated
immune responses (6)

Hypersensitivity, Antibody Dependent Cell Cytotoxicity; Cytokines-properties,

receptors and therapeutic uses; Antigen processing and presentation Hapten-carrier
system. Complement system. (4)

Antigen – Antibody Interaction dependent Techniques

Precipitation, Agglutination; Advanced immunological techniques- RIA, ELISA,

Western blotting, ELISPOT assay, Immuno-electron microscopy and
Immunoflourescence techniques (6)

Clinical Immunology

Preparation and clinical uses of Monoclonal and Polyclonal antibody. (3)

Transplantation; Autoimmunity; (5)

Vaccination: Principles and development of vaccines against different diseases. (3)

21
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Text Books, Textbook:

and/or 1. Kuby J, Thomas J. Kindt, Barbara, A. Osborne Immunology, 6th Edition,
reference Freeman, 2002.
2. Janeway et al., Immunobiology, 4th Edition, Current Biology publications. 1999
material
Reference Books:
1. Brostoff J, Seaddin JK, Male D, Roitt IM., Clinical Immunology, 6th Edition,
Gower Medical Publishing, 2002.
2. Paul, Fundamental of Immunology, 4th edition, Lippencott Raven, 1999.
3. Goding, Monoclonal antibodies, Academic Press. 1985.

CO-PO MAPPING:

Course Code: BTC402 Course Title: IMMUNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2

CO2 2 2

CO3 2 2 2 2

CO4 3 3 2 1 2 3

CO5 3 3 3 1 2 3

Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
CSC431 PROGRAMMING PCR 3 0 0 3 3
AND DATA
STRUCTURE
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
Knowledge of Programming CT+EA
Language
Course  CO1: Understanding of the fundamental concepts of data, data types and
Outcomes abstract data types.
 CO2: Implementation of different abstract data types using different data
22
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

structures.
 CO3: Apply different types of data structures to implement different
solutions to problems.
 CO4: Analysis of the suitability/compatibility of different data structures
based on the types of applications.
Topics 1) Introduction: Basic terminology, elementary data organization, structure
Covered operations, algorithm, complexity and time-space trade-off.
[2]
2) Arrays: Array definition, representation and analysis, single and
multidimensional arrays, address calculation, application of arrays,
character string in c, character string operation, array as parameters, ordered
list, sparse matrices and vectors.
[4]
3) Stacks: Array representation and implementation of stack, operations on
stacks: push AND pop, array representation of stack, linked representation
of stack, operations associated with stacks, application of stack: conversion
of infix to prefix and postfix expressions, evaluation of postfix expression
using stack.
[5]
4) Queues: Array and linked representation and implementation of queues,
operations on queue: create, add, delete, full and empty, circular queues, d-
queues and priority queues.
[4]
5) Linked list: Representation and implementation of singly linked lists, two-
way header list, traversing and searching of linked list, overflow and
underflow, insertion and deletion to/from linked lists, insertion and deletion
algorithms, doubly linked list, linked list in array, polynomial
representation and addition, generalized linked list, garbage collection and
compaction. [7]
6) Trees: Basic terminology, binary trees, binary tree representation, algebraic
expressions, complete binary tree, extended binary trees, array and linked
representation of binary trees, traversing binary trees, threaded binary trees,
traversing threaded binary trees.
[7]
7) Searching: Sequential search, binary search.
[2]
8) Sorting: Insertion Sort, Selection Sort, Bubble Sort, Radix Sort, Quick Sort,
Merge Sort and Heap Sort. [8]
9) Binary Search Trees: Binary Search Tree (BST), Insertion, Deletion and
Search Operations in BST.
[5]
10) Height Balance Tree: Introduction to Height Balance Tree, Insertion,
Deletion and Search Operations in Height Balance Tree.
[5]
23
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

11) Graphs: Terminology and representations, graphs and multi-graphs,

directed graphs, sequential representations of graphs, adjacency matrices,
traversal, connected component and spanning trees, minimum cost spanning
trees. [7]

Text Books, TEXT BOOKS:

and/or
reference 1. Horowitz and Sahani, “Fundamentals of data Structures”, Galgotia
material Publication Pvt. Ltd., New Delhi.
2. R. Kruse etal, “Data Structures and Program Design in C”, Pearson
Education Asia, Delhi-2002
3. A. M. Tanenbaum, “Data Structures using C & C++”, Prentice-Hall of
India Pvt. Ltd., New Delhi

REFERENCE BOOKS:

1. Bruno R Preiss, “Data Structures and Algorithms with Object Oriented

Design Pattern in C++”, Jhon Wiley & Sons, Inc.
2. 6. Adam Drozdek, “Data Structures and Algorithms in C++”, Thomson
Asia Pvt. Ltd.(Singapore)

CO-PO MAPPING:

Course Code: CSC431 Course Title: PROGRAMMING AND DATA STRUCTURE

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1 0 1 0 0 0 0 1 1 0 3

CO2 2 3 3 1 0 0 0 1 2 2 1 2

CO3 2 3 3 3 1 1 0 1 2 2 2 3

CO4 3 3 3 3 2 2 2 2 3 3 3 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTO FOOD PER/OER 3 0 0 3 3

441 BIOTECHNOLOGY

Pre-requisites Life science- BTC-01

24
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

BTC-01

Course CO1: To quantitate and identify the spoilage microorganisms present in food.
Outcomes CO2: To learn the concepts of food fermentation and increase the shelf life of food.
CO3: To learn the concepts in genetically modified food and increase the agricultural
yield by using genetic engineering approach.
CO4: To apply the concepts of antioxidant and nutraceutical for health and wellness.
CO5: Tofollow the regulations and ethical issues of food safety by using good
manufacturing practices in industry andgenetically modified food.

Topics Food Microbiology: [8]

Covered
Microorganism in food, Intrinsic and extrinsic parameters of food, rapid methods
for identification of microorganism in food, Food borne illness, Biosensors –use
and application

Food preservation [8]

Pasteurization, sterilization, Canning, thermal process of food with numericals,

Irradiation, Dehydration, low temperature , use of preservatives

Food fermentation [10]

Role of lactic acid bacteria in fermentation and strain improvement,

Fermentation of meat, fish, vegetables, beverages, dairy product, non-beverage

product , use of genetic engineering techniques for improved quality product.

Genetically modified food [8]

Fruit ripening, amino acid, vitamin content, Golden rice. Safety aspects of
genetically modified food, Ethical and regulatory issues

Biotechnology in relation to food product [4]

Antioxidant, nutraceutical,

Food safety [6]

Legal status of irradiated food and preservatives, Concept of HACCP, Hazop,

codex alimentarius, ISO series, detection of toxin, heavy metal , pesticide and
herbicides

25
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Text Books, Text Book

and/or
Food microbiology by James . M. Jay
reference
material Food Microbiology by Frazier and Westhoff

Plant Biotechnology by Slater

Reference Book

Fundamentals of Food Biotechnology by Lee

CO-PO MAPPING:

Course Code: BTO441 Course Title: FOOD BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 3 3 3 3 2 1 1 2 3

CO2 3 3 3 3 2 2 3 2 1 1 2 3

CO3 3 3 3 3 3 3 3 3 2 1 2 3

CO4 3 2 3 3 1 3 3 2 2 1 1 3

CO5 3 2 2 2 3 3 3 3 3 3 3 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTS451 CELL BIOLOGY PCR 0 0 3 3 1.5

AND
GENETICS
LABORATORY

Pre-requisites BTC301

Cell Biology and Genetics

(BTC301)

26
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course CO1: To design, analyze and solve problems related to cell biology and Molecular
Outcomes genetics and interpretation of data obtained by the lab experiments.

CO2: To develop skills to perform experiments related to cell biology and Molecular
genetics and have hands on training on the related area.

CO3: To learn to interpret data, draw conclusion and develop trouble shooting
skills.

Topics 1. Isolation of chromosomal DNA from mammalian cells.

Covered 2. Genotyping PCR of a genetically modified cell.
3. Isolation of mRNA and RT-PCR to determine the level of transcription of the
gene.
4. Studying to detect variations like single nucleotide polymorphism.
5. Studying bacterial conjugation.
6. To examine the morphology of cells
7. Identification of cellular organelles by staining method
8. Cell proliferation assay
9. Cell adhesion assay
10. Cell migration assay

Text Books, TEXT BOOKS:

and/or
reference REFERENCE BOOKS:
material  Molecular Biology of Cell by Albert et.al. John Wiley & Sons
 The Cell by Cooper. ASM Press
 M.W.Strickberger: Genetics, Pearson.

CO-PO MAPPING:

Course Code: BTS451 Course Title: CELL BIOLOGY AND GENETICS LABORATORY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 3 2 2 1 2 2 2 1 3

CO2 3 2 2 3 3 3 1 2 3 1 1 3

CO3 3 3 2 2 2 3 1 3 2 3 1 3

27
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lectur Tutoria Practical Total
(PCR) / e (L) l (T) (P) Hour
Electives s
(PEL)
CHS4 UNIT PCR 0 0 3 3 3
81 OPERATIONS
OF CHEMICAL
ENGINEERING
LABORATORY I

CHC431: Unit operations of Course Assessment methods (Continuous (CT) and end assessment
chemical engineering-I. (EA))

CT+EA
Course CO1: To record observations systematically and arrive at required results based on
Outcomes experiments conducted

CO2. Understand the principles, laws and mechanism of different comminuting

methods like sieve analysis crushers, and grinders, ball mill
CO3. Acquire the knowledge of a cyclone separator and its efficiency
CO4. Acquire the knowledge of different flow regime measuring instruments.
CO5. Study and design different flow measuring instruments.
Topics  To find out the reduction ratio and capacity and to verify the laws of crushing by Jaw
Covered Crusher.
 To determine the optimum speed for maximum new surface area created for the
given feed size and also determines the critical speed of the ball mill.
 Demonstration of the operation of a cyclone separator and determination of its
overall efficiency
 Experiments on Reynolds Apparatus for determination of flow regime and
construction of Fanning friction factor vs. Reynolds No. plot
 Determination of co efficient of Discharge for Orifice meter and Discharge for
Venturi meter.
 Determination of co-efficient of Pitot tube and construction of velocity profile across
the cross section of pipe.
 Experiment to prove Bernoulli’s equation for fluid flow
 To analyze a given powder for its particle size distribution. / Cumulative and
Differential methods of particle size distributions and to find out screen efficiency

28
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Text 1. Units Operations of Chemical Engineering: McCabe & Smith and Harriot, MGH
Books, 2. Coulson, J.M., Richardson, J.F., “Chemical Engineering”, Volume 2, Third
and/or Edition, Pergamon Press, 1977
reference
3. Principles of Unit Operations by Alan S Foust, L.A. Wenzel, C.W. Clump, L.
material
Maus, and L.B.

CO-PO MAPPING:

Course Code: CHC431 Course Title: UNIT OPERATIONS OF CHEMICAL

ENGINEERING I

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 3 3 3 3 3 1 3 1 3 2

CO 2 3 3 3 3 3 2 3 1 3 2

CO 3 3 3 3 3 3 2 3 1 3 2

CO 4 3 3 3 3 3 1 2 3 1 3 2

CO 5 3 3 3 3 3 1 2 3 1 3 2

Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
CSS481 PROGRAMMING PCR 0 0 3 3 2
AND DATA
STRUCTURE
LABORATORY
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
Knowledge of Programming CT+EA
Language
Course  CO1: Choose appropriate data structures for representation and manipulation of
Outcomes the data for the given problems.
 CO2: Handle operations like search, insertion, deletion, traversing and sorting
on various data structures.
 CO3: Have knowledge on the applications of linear and non-linear data
structures for real life problems.
 CO4: Able to store and manipulate data in an efficient manner.
 CO5: Able to implement stack, queue, binary tree, etc. using arrays and linked
lists.
 CO6: Able to apply the concepts learnt through thiscourse in various domains
29
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

like DBMS and compiler.

Topics Linked List

Covered  Implementations of Linked Lists menu driven program
 Implementation of different operations on linked list – copy, concatenate, split,
reverse,
count no. of nodes etc.
 Representation of Sparse matrix using multilinked structure. Implementation of
sparse
matrix addition and multiplication
 Implementation of polynomial operations (addition, subtraction) using Linked
List
 Implementations of Doubly Linked List
Stack
 Implementations of stack menu driven program using array and linked list
 Implementation of multi-stack in one array
 Implementations of Infix to Postfix Transformation and its evaluation program
 Implementations of Infix to Prefix Transformation and its evaluation program
Queue
 Implementations of double ended queue menu driven program using array and
linked list
 Implementations of circular queue menu driven program using array and linked
list
 Implementation of Priority queue program using array
Tree
 Implementations of Binary Tree menu driven program
 Implementation of Binary Tree Traversal program
 Implementations of BST program
 Implementation of various operations on tree like – copying tree, mirroring a
tree,
counting the number of nodes in the tree, counting only leaf nodes in the tree
Sorting
 Implementations Insertion sort, Selection sort, Bubble sort and Quick sort
menu driven program
Searching
12) Implementations of Sequential and Binary Search menu driven program

Text TEXT BOOKS:

Books,
and/or 4. Horowitz and Sahani, “Fundamentals of data Structures”, Galgotia Publication
reference Pvt. Ltd., New Delhi.
material 5. R. Kruse etal, “Data Structures and Program Design in C”, Pearson Education
Asia, Delhi-2002
6. A. M. Tanenbaum, “Data Structures using C & C++”, Prentice-Hall of India
Pvt. Ltd., New Delhi

REFERENCE BOOKS:

3. Bruno R Preiss, “Data Structures and Algorithms with Object Oriented Design
Pattern in C++”, Jhon Wiley & Sons, Inc.
30
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

4. 6. Adam Drozdek, “Data Structures and Algorithms in C++”, Thomson Asia

Pvt. Ltd.(Singapore)

CO-PO MAPPING:

Course Code: CSS481 Course Title: PROGRAMMING AND DATA STRUCTURE

LABORATORY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 2 0 0 0 1 1 0 3 3

CO2 3 2 3 0 1 0 0 0 0 0 3 3

CO3 3 1 3 0 0 0 0 0 1 0 3 2

CO4 3 3 2 2 0 0 0 0 1 0 3 3

CO5 2 2 2 1 1 0 0 0 0 0 2 2

CO6 3 3 2 2 2 0 0 0 1 0 3 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR)
/ Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTC BIOCHEMICAL PCR 3 1 0 4 4

501 REACTION
ENGINEERING
AND
BIOREACTOR
DESIGN

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))

CT+EA

Course CO1 – To gain knowledge about Chemical and Biochemical processes, order of

31
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Outcomes reactions, effect of various parameters on rate constant of a reaction

C02- To study about different reactions in batch reactors, kinetics of enzyme

catalyzed reactions

CO3- To acquire knowledge about different ideal and non-ideal reactors, reaction
kinetics, microbial growth kinetics

CO4- To learn about various types of Bioreactors, their design considerations and
applications in the field of Biochemical Engineering

CO5- To study about mass transfer in bioprocess systems, scale up, instrumentation
and control, bioreactor considerations in plant and animal cell culture

Topics Rate of chemical reaction; Effect of Temperature on Rate Constant, Arrehnius

Covered equation, Order and Molecularity of a Chemical reaction, Elementary Reactions,
First, Second and Third order reactions, Pseudo-first order reaction, Determination of
rate constant and order of reaction. [5]
Interpretation of batch reactor data for simple and complex reactions. Kinetics of
Enzyme catalyzed reactions for free and immobilized enzymes.–derivation of
Michaelis-Menten equation, Briggs-Haldane relationship, the determination and
significance of kinetic constants, Lineweaver-burk and Eadie-Hofstee plot, principles
of enzyme inhibition – Competitive, noncompetitive and uncompetitive.
[5]
Fundamentals of homogeneous reactions for batch, plug flow and mixed flow
reactors. [5]

Concept of ideal and non ideal reactors, Residence time distribution, Models for non
ideal reactors (Dispersion model, tanks-in-series model).
[5]

Stoichiometry of cellular reactions. Microbial growth kinetics (Batch, continuous, fed

batch). Monod model and other kinetic models. Growth kinetics with plasmid
instability.
[6]

Bioreactor design: Packed bed bioreactor, Fluidized bed bioreactor, Bubble column
bioreactor, Air lift bioreactor, Tower bioreactor. Hollow fiber bioreactor, Membrane
bioreactor. [4]

Design of fermenter. Fermenter utilities – boiler and refrigeration system.

[5]

Immobilized cell bioreactor system. Mass transfer in bioprocess system. Two film
theory, Kla determination. Scale up concepts. Bioreactor considerations for plant and
animal cell culture
[5]
Bioprocess instrumentation and control. Computer controlled bioreactors.
[2]

32
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Text TEXT
Books, 1. Bioprocess Engineering: Basic Concepts (2nd Edition), Shuler and Kargi,
and/or Prentice Hall International.
2. Bioprocess Engineering Principles – Pauline M Doran. Academic press
reference
3. Chemical Reaction Engineering ,O Levenspiel, Wiley
material 4. Principles of Fermentation Technology, Stanbury and Whitaker, Pergamon
press

REFERENCE
1. Biochemical Engineering. Fundamentals, Bailey &Olis, McGraw-Hill
Biochemical Engineering, Humphrey and Aiba. Academic Press

CO-PO MAPPING:

Course Code: BTC501 Course Title: BIOCHEMICAL REACTION ENGINEERING AND

BIOREACTOR DESIGN

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 2 1 1 1 1 1 1 1 2

CO2 3 2 2 1 1 1 1 1 1 1 2

CO3 3 2 2 1 1 1 1 1 1 1 2

CO4 3 2 2 1 1 1 1 1 1 1 2

CO5 3 2 2 1 1 1 1 1 1 1 2

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTC502 CELL AND PCR 3 1 0 4 4
TISSUE
CULTURE
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
BTC01 Life Science CT+EA
BTC301 Cell Biology and
Genetics
Course CO1: Students will acquire knowledge on plant and animal cell and tissue growth
Outcomes
33
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

conditions.
CO2: Students will be acquainted with plant and animal cell and tissue culture
techniques in laboratory and industry setups.
CO3: Students will be proficient in applying basic understanding of plant and
animal cell and tissue growth requirements in plant and animal tissue culture
techniques.
Topics 1. Introductory history, plant & animal cell culture facilities laboratory
Covered organization, media & aseptic conditions. [2]
2. Plant growth hormones, Cell culture, cellular totipotency, somatic
embryogenesis, anther, pollen and ovary cultures, protoplast culture. [6]
3. Haploid production, triploid production, in vitro pollination and fertilization,
zygotic embryo culture, somatic hybridization and cybridization, genetic
transformation, somaclonal and gametoclonal variant selection. [7]
4. Production of disease-free plants, clonal propagation. [3]
5. Industrial applications: secondary metabolite production, germplasm
conservation. [3]
6. Animal Cell Culture: Historical Background. [1]
7. Importance of and progress in Animal Cell Culture Technology. [1]
8. Biology of Animal Cell; Cellular Interactions. [5]
9. Importance of Serum and Serum Free Media. [2]
10. Culturing and Sub-Culturing of Animal Cells. [3]
11. In Vitro Transformation of Animal Cells. [1]
12. Cell Differentiation & Cell Movement. [2]
13. Cloning of Animal Cells. [2]
14. Cell Line Preservation. [1]
15. Cell Line Characterization. [2]
16. Chromosome Spreading and Karyotype Analysis. [2]
17. Mycoplasma: Detection and Control. [1]
18. Monoclonal Antibody Production. [2]
19. Insect Cell Culture: An Overview. [2]
Text Books, Text Book:
and/or 1. Razdan – Introduction to Plant Tissue Culture, 2nd edition, 2007, Oxford and
reference IBH Publishing.
material
2. “Culture of AnimalCells: A manual of basic technique”, 4 th Edition
Author(s)/Editor(s): Freshney RI. Publisher: WIELY-LISS ISBN:0-471-34889-
9.
Reference Book:
1. Bhojwani and Razdan –Plant Tissue Culture: Theory and Practice, a revised
edition, 2009, Elsevier.

34
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

2. Jha and Ghosh – Plant Tissue Culture: Basic and Applied, revised 2nd edition,
2016, Platinum Publishers.

CO-PO MAPPING:

Course Code: BTC502 Course Title: CELL AND TISSUE CULTURE

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1 1 1 1 1

CO2 2 1 1 1 1 1

CO3 1 2 1 1

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTC503 BIOSEPARATION PCR 3 1 0 4 4
AND
BIOCHEMICAL
ANALYSIS
Pre-requisites Course Assessment methods (Continuous assessment (CA) and
end-term examination (ET))
Basic Physics, Mathematics CA+ET
including basics of Differential
& Integral Calculus, Basic
concepts of Chemistry &
Biochemistry
Course  CO1: To learn the concepts of separation including purification sequence
Outcomes and its monitoring and the properties of proteins underlying
bioseparations.
 CO2: To learn techniques of biochemical analysis of biomolecules.
 CO3: To learn and analyze, mathematically wherever applicable, the
various unit operations in bioseparation.
 CO4: To understand the design aspects of unit operations in
bioseparation.
CO5: To solve problems of bioseparations including industrial bioseparations.

Topics Basic Concepts [3]

Covered Basic concepts of Bio-separation Technology

35
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Basic Analytical Tehniques: [10]

Introduction to Biomolecules, Buffers
Estimation of carbohydrate, protein, and lipid, and enzyme assay
Quantitation of DNA and RNA
Methods of cell disintegration

Removal of Insolubles [9]

Flocculation and conditioning of broth. Filtration at constant pressure and at
constant rate; equations for batch and continuous filtration, centrifugal and cross-
flow filtration.
Centrifugation: basic principles, design characteristics; ultracentrifuges: principles
and applications.

Techniques Involved in Separation Processes for Solutes [9]

Foam-fractionation; Solvent extraction, aqueous two-phase extraction, adsorption
& desorption processes; Salt precipitation

Membrane based separation processes:Micro-filtration, Dialysis, Reverse osmosis,

Ultrafiltration and affinity ultrafiltration, concentration polarization, rejection, flux
expression, membrane modules, dead-end and cross-flow modes.

Advanced Techniques for Bioseparation: [9]

Chromatography: paper chromatography, TLC, gel filtration, ion exchange,
hydrophobic interaction chromatography, affinity chromatography, HPLC.

Electrophoresis: Theory and application of Polyacrylamide and Agarose gel

electrophoresis; 2D-Gel electrophoresis

Industrial Application with an example [2]

Text Books, Textbooks :

and/or 1. Practical Biochemistry Principles and techniques (5 thed)/ Principles and
reference Techniques of Biochemistry and Molecular Biology (7 thed): Editor
material Wilson and Walker, Cambridge University Press
2. Geankoplis, Transport Processes & Unit operations, PHI.
Reference books:
1. D. Holme & H. Peck, Analytical Biochemistry, 3 rded, Longman, 1998
2. Shuler & Kargi, Bio-process Engg. PHI
3. Bailey &Olis, Biochemical Engg. Fundamentals, McGraw-Hill

CO-PO MAPPING:

Course Code: BTC503 Course Title: BIOSEPARATION AND BIOCHEMICAL

ANALYSIS

PO1 PO2 PO3 PO 4 PO5 PO6 PO 7 PO 8 PO 9 PO10 PO 11 PO 12

CO 1 1 1 - - - 1 1 1 - 2 - -

36
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO 2 1 2 - 2 1 1 - 1 1 2 - 1
CO 3 2 3 1 - - - - - 1 2 - -
CO 4 1 - 2 - 1 - 1 - 2 2 1 -
CO 5 3 2 3 1 - 1 1 1 2 2 1 2

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
CHC531 UNIT PCR 3 1 0 4 4
OPERATIONS
OF CHEMICAL
ENGINEERING-
II

CHC431: Unit operations of Course Assessment methods (Continuous (CT) and end assessment
chemical engineering-I. (EA))

CT+EA
Course  To learn different types of mass transfer phenomena
Outcomes  Understanding the fundamentals of mass transfer operations
 To learn design parameters, their effects and calculations
 To compare different types of mass transfer operations and their
applications
 To solve related problems of different difficulty levels through tutorials

Topics Module I: Principles of mass transfer: Introduction, diffusion, classification of

Covered diffusion, Inter-phase mass transfer. [8 hr]

Module II: Evaporation: Introduction, types of evaporators, design calculation and

processes [8 hr]

Module III: Drying: Principles of drying, drying characteristics, methods,

equipment. Humidification and Dehumidification: Definitions, adiabatic saturation
temperature, wet bulb temperature, processes [8 hr]

Module IV: Absorption: Principle, operation and design calculation [8 hr]

Module V: Distillation: Flash distillation, differential distillation, fractionation and

design calculations [8 hr]

37
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Module VI: Extraction and Adsorption: Principles and Operations. [8 hr]

Text Books, Text Books:

and/or 1. B.K.Dutta, Principles of Mass Transfer and Separation Processes, Prentice
reference Hall India Private Limited
material 2. N Anantharaman and K.M.M.S. Begum, Mass Transfer theory and practice.
Prentice Hall India Private Limited
3. Robert E. Treybal, Mass Transfer Operations, McGraw Hill limited

CO-PO MAPPING:

Course Code: CHC531 Course Title: UNIT OPERATIONS OF CHEMICAL

ENGINEERING-II

PO1 PO2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO9 PO10 PO11 PO 12

CO 1 1 3 3 3 2 1 1 0 3 3 1 3

CO 2 1 3 3 3 2 1 1 0 3 3 1 3

CO 3 1 3 3 3 2 1 1 0 3 3 1 2

CO 4 3 3 3 3 2 1 1 0 3 3 1 3

CO5 1 2 2 3 2 1 1 0 3 3 1 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTO540 MINERAL PEL 3 0 0 3 3

BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course  CO1: To understand the nature and characteristics of different

Outcomes biogeochemical cycles and involvement important micro-organisms.

 CO2: To learn the basic concepts of bioleaching and biobeneficiationalong

38
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

with the microbiological aspects

 CO3: To gain the detail knowledge bioleaching processes with examples.

 CO4: To demonstrate and provide examples on how to use microbes for

the environmental pollution control

Topics Module-I :
Covered
Introduction to Biotechnology applied to Raw Material processing,
Biogeochemical reactions – chemical mechanisms and controlling factors,
Microbial interventions, Nature and characteristics of Biogeochemically important
micro-organisms. 10

Module-II:

Kinetics of bioleaching; Applications of biogeochemical process in mining and

metallurgy, dump, heap and in-situ leaching.
8

Module-III:

Reactor modeling for leaching, Beneficiation of ored and process residues:

recovery of gold, silver, copper, beneficiation of sulfidic tailings from tin
processing; purification of ferroginous sand.
8

Module-IV :

Beneficiation of bauxite, applications of sulphate reducing bacteria; applications

of sulphate reducing bacteria, Environmental pollution control: accumulation of
metals by microbial cells.
8

Text Books, Books:

and/or
1. H.D. Kumar and S.Kumar , Modern Concepts of Microbiology , Vikas
reference
Publishing House , 2nd Edition , 2001
material 2. M.E. Curtin , Microbial mining and metal recovery biotechnology (1) , pp
229-235 , 1983
Woods D, Rawling D.E., Bacterial bleaching and biomining J.L.(ed), Revolution
in biotechnology , Cambridge University Press.

39
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING:

Course Code: BTO540 Course Title: MINERAL BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1 1 - 2 1 1 - 1 - - 1

CO2 2 1 1 - 1 - 2 1 1 1 - 1

CO3 2 1 1 1 1 - 1 - 1 - - 1

CO4 2 1 1 1 1 - 2 1 1 1 1 1

Department of
Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lect Tutorial Practical Total
(PCR) ure
/ (L) (T) (P) Hours
Electives
(PEL)
BTO541 INTRODUCTION PEL 3 0 0 3 3
TO
COMPUTATIONAL
BIOLOGY
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment
(EA))
Life Science BTC01 CT+EA
Course  CO1: To impart knowledge of life science and biologicaldata
Outcomes  CO2: To acquire knowledge of computational and mathematical skills
for addressing important biologicalquestions.
 CO3: To learn how to develop and implement computational algorithms
and tools for processing biologicaldata

40
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Topics 1. Introduction to Computational biology and its applications(2)

Covered 2. Central dogma and biological macromolecules- DNA, RNA & proteins(2)
3. Major biological databases related to DNA, RNA, proteins
&metabolic pathways(3)
4. Basic file formats & sequence representation(2)
5. Computational algorithms for Sequence Alignment: Local and global
alignment, Sequence similarity, Sequence identity, Gaps, Scoring
matrices, pairwise and multiple alignments, Dynamic programming,
BLAST & its application,(7)
6. Algorithms for phylogenetics: Tree constructions(5)
7. StructuralBioinformatics:
A. Protein Structure and itsvisualization(2)
B. Protein structural alignment(3)
C. Protein secondary Structure Prediction(4)
D. Protein tertiary Structure Prediction(4)
E. RNA Structure Prediction(3)
F. Molecular docking and docking algorithms(3)
7. Application of machine learning in biological sciences (Basic concepts) (2)

Text Books, Text Books:

and/or 1. Bioinformatics: Sequence and Genome Analysis by David W Mount,
reference Cold Spring Harbor LaboratoryPress
material 2. Introduction to Bioinformatics by Arthur MLesk

Reference Books:
1. Protein bioinformatics: an algorithmic approach to sequence and
structure analysis by Ingvar Eidhammer, Inge Jonassen and William
R.Taylor.
2. Essentials of Bioinformatics by JinXiong

CO-PO MAPPING:

Course Code: BTO541 Course Title: INTRODUCTION TO COMPUTATIONAL

BIOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 1 1 1 1

CO2 3 3 2 2 1 2

CO3 3 3 2 2 3 1 1 3 1 2 1

41
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR)
/ Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTS 551 IMMUNOLOGY PCR 0 0 3 3 1.5

LABORATORY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To learn the fundamentals of immunological techniques

Outcomes
CO2: To be able to perform techniques routinely used in immunology, particularly
the use of specific antibody in biomolecular applications.

CO2: To be able to isolate, count and identify different types of blood cells.

CO4: To develop an idea for proper documentation of the work including laboratory
procedures, experimental conditions, materials used, equipment used and the results.

CO5: To understand the basic hazards of working with human samples and antigens
and safety measures to be taken

Topics 1. Cell count with Haemocytometer

Covered 2. Determination of viability of the cells
3. Serology: Preparation of the blood smear
4. Blood cell identification
5. Blood grouping by Agglutination assay
6. Quantitative WIDAL test (By tube test and slide test)
7. Precipitation test: Immunodiffusion
8. Enzyme linked Immunosorbent Assay (ELISA)
9. Protein detection by Western blot technique.
10. Lymphocytes isolation using FicollHypaque technique

Text Books, 1. Immunology Laboratory manual.

and/or 2. ArtiNigam,ArchanaAyyagari,“Lab Manual in Biochemistry, Immunology and
reference Biotechnology”,
Mc Graw Hill Education, India, 2007
material

42
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING :

Course Code: BTS551 Course Title: IMMUNOLOGY LABORATORY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 2 1 2

CO2 2 2 1 1 2

CO3 2 1 1 2 1 1

CO4 1 3 2

CO5 2 2 2

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTS- BIOPROCESS PCR 0 0 3 3 1.5
552 TECHNOLOGY
LABORATORY
Pre-requisites Course Assessment methods (Continuous evaluation (CE) and end
assessment (EA))
CE+EA
Course CO1: To learn about surface culture fermentation in lab scale
Outcomes CO2: To learn about submerged culture fermentation in lab scale and various assays
for antibiotics production, polysaccharide production and cell growth determination
CO3: To learn about cell immobilization by entrapment method

Topics 1. Production of neomycin by fermentation

Covered 2. Production of citric acid by fermentation
3. Production of xanthan/dextran gum by fermentation
4. Production of Bakers yeast by fermentation
5. Cell Immobilization by entrapment method
Text Books, Experimental Process Biotechnology Protocols, S N Mukhopadhyay, Viva Books,
and/or 2007.
reference
material

43
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING :

Course Code: BTS552 Course Title: IMMUNOLOGY LABORATORY

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 1 1 1 1 2 3 2 2

CO2 1 1 1 1 2 3 2 2

CO3 1 1 1 1 2 3 2 2

Department of Chemical Engineering

Course Title of the course Program Total Number of contact hours Credit
Code Core Lectur Tutoria Practical Total
(PCR) / e (L) l (T) (P)# Hour
Electives s
(PEL)
CHS581 UNIT PCR 0 0 3 3 1.5
OPERATIONS
OF CHEMICAL
ENGINEERING
LABORATORY
II
Pre-requisites Course Assessment methods (Continuous evaluation (CE) and
end assessment (EA))
Unit operation of Chemical CE+EA
Engineering I and II
Course  CO1: Apply the knowledge of fundamentals of heat and mass transfer equipment on
Outcom laboratory
es  CO2: Experimentation and data analysis
 CO3:To apply principles of mass transfer phenomena to chemical process industries
 CO4: Handling various instruments and solve various difficulty levels
 CO5: Learn industrial applications of heat transfer equipment
 CO6: Complete process design through assignment / group task
Topics  Determination of thermal conductivity of metal rod
Covere  Determination of overall heat transfer coefficient in a counter-current & parallel
d flow double pipe heat exchanger.
 Determination of overall heat transfer coefficient in a shell and tube heat exchanger.
 Experimental test rig on drop-wise and film-wise condensation for assessing the
performance.
 Studies on estimation of hold-up volume under steady state condition and evaluate
the overall performance of a rotary dryer.
 Determination of overall efficiency of cooling tower
 Estimation of rate of drying of specific biomass under steady state condition in a
atmospheric tray dryer
 Performance studies on continuous fractionating distillation column in terms of
44
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

distillate, bottom product and reflux quantities, % loss, % recovery, energy

consumption etc.
36 hr
Text Suggested Text Books:
Books, 1) Transport Processes and Unit Operations - C. J. Geankoplis
and/or 2) Heat Transfer: Principles and Applications: B. K Dutta
referen
ce
materia
l

CO-PO MAPPING:
Course Code: CHS581 Course Title: UNIT OPERATIONS OF CHEMICAL
ENGINEERING LAB-II

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 3 3 3 3 3 1 3 1 3 2

CO 2 3 3 3 3 3 2 3 1 3 2

CO 3 3 3 3 3 3 2 3 1 3 2

CO 4 3 3 3 3 3 1 2 3 1 3 2

CO 5 3 3 3 3 3 1 2 3 1 3 2

Department of Humanities and Social Sciences

Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
HSC631 ECONOMICS PCR 3 0 0 3 3
AND
MANAGEMENT
ACCOUNTANCY
Pre-requisites Course Assessment methods (Continuous evaluation (CE) and end
assessment (EA))
NIL CE+EA

Course  To review basic economic principles with students;

Outcomes  To introduce students basic capital appraisal methods used for carrying out
economic analysis of different alternatives of engineering projects or works;
 To educate the students on how to evaluate systematically the various cost
elements of a typical manufactured product, an engineering project or service,

45
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

with a view to determining the price offer.

Topics PART 1: Economics

Covered Group A: Microeconomics
Sl. No. Name L T P Cr H
Unit 1: Economics: Basic Concepts 2 0 0 2 2
Unit 2: Theory of Consumer Behaviour 3 0 0 3 3
Unit 3: Theory of Production, Cost and Firms 3 0 0 3 3
Unit 4: Analyses of Market Structures: Perfect Competition 3 0 0 3 3
Unit 5: Monopoly Market 1 0 0 1 1
Unit 6: General Equilibrium &Welfare Economics 2 0 0 2 2
TOTAL 14 0 0 14 14

Group B: Macroeconomics
Sl. No. Name L T P Cr H
Unit 1: Introduction to Macroeconomic Theory 2 0 0 2 2
Unit 2: National Income Accounting 3 0 0 3 3
Unit 3: Determination of Equilibrium Level of Income 4 0 0 4 4
Unit 4: Money, Interest and Income 2 0 0 2 2
Unit 5: Inflation and Unemployment 2 0 0 2 2
Unit 6: Output, Price and Employment 2 0 0 2 2
TOTAL 15 0 0 15 15

PART 2: Accountancy
Sl. No. Name L T P Cr H
Unit 1: Introduction to Accounting 2 0 0 2 2
Unit 2: Primary Books of Accounts (Journal) 1 0 0 1 1
Unit 3: Secondary Books of Accounts (Ledger) 3 0 0 3 3
Unit 4: Cash Book 2 0 0 2 2
Unit 5: Bank Reconciliation Statement 1 0 0 1 1
Unit 6: Trial Balance 2 0 0 2 2
Unit 7: Final Accounts 2 0 0 2 2
TOTAL 13 0 0 13 13
Text Books, PART 1: Economics
and/or Group A: Microeconomics
reference 1. Koutsoyiannis: Modern Microeconomics
material 2. Maddala and Miller: Microeconomics
3. AnindyaSen: Microeconomics: Theory and Applications
4. Pindyck&Rubenfeld: Microeconomics

Group B: Microeconomics
1. W. H. Branson: Macroeconomics – Theory and Policy (2nd ed)
2. N. G. Mankiw: Macroeconomics, Worth Publishers
3. Dornbush and Fisher: Macroeconomic Theory
4. SoumyenSikder: Principles of Macroeconomics

PART 2: Accountancy
1. Gupta, R. L. and Radhaswamy, M: Financial Accounting; S. Chand & Sons
2. Ashoke Banerjee: Financial Accounting; Excel Books
3. Maheshwari: Introduction to Accounting; Vikas Publishing
46
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

4. Shukla, MC, Grewal TS and Gupta, SC: Advanced Accounts; S. Chand & Co.

CO-PO MAPPING:

Course Code: HSC631 Course Title: ECONOMICS AND MANAGEMENT

ACCOUNTANCY

CO
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 - - 1 - - 3 - - 3 2 1 -
CO2 3 2 - 1 - 2 - 2 - - 3 1
- - - -
CO3 1 - 3 - - - 2 -

Department of Biotechnology
Course Title of the course
Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTC601 BIOINFORMATICS PCR 2 1 0 3 3

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
Cell Biology and Genetics CT+EA
(BTC301), Biochemistry and
Enzyme Technology (BTC303),
Programming and Data Structure
(CSC431)
Course  CO1: To learn how to integrate both biological and computer skills for
Outcomes addressing important biological questions.
 CO2: To acquire knowledge of existing biological databases and understand the
methods for storing, organizing, retrieving and analyzing biological data in an
efficient way.
 CO3: To learn and implement computational algorithms and tools (webservers
and standalone programs) for processing biological data
Topics 1. Introduction to Bioinformatics and its applications (2)
Covered 2. Linux and Bash programming for bioinformatics (3)
3. Major Information Resources & biological databases (3)
4. Sequence Alignment: Sequence similarity, Sequence identity, Sequence
homology, Gap Penalty, local and global alignment, pairwise and multiple
alignments, sequence alignment algorithm, Dynamic programming, BLAST
and PSI-BLAST, Application of BLAST tool, Concept of Scoring matrix
(5)
5. Molecular phylogeny and evolution: Phylogenetics basics and methods for
47
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

phylogenetic tree constructions (4)

6. Structural Bioinformatics:
A. Protein Structure and its visualization, structural alignment (3),
B. Protein secondary Structure Prediction (2),
C. Protein tertiary Structure Prediction (2),
D. RNA Structure Prediction (2)
7. Molecular Docking and Drug design (Basic concepts) (2)

Text Books, Text Books:

and/or 1. Bioinformatics: Sequence and Genome Analysis by David W Mount, Cold
reference Spring Harbor Laboratory Press
material 2. Introduction to Bioinformatics by Arthur M Lesk

Reference Books:
1. Introduction to Bioinformatics computer Skills by Cynthia Gibas and Per
Jambeck
2. Protein bioinformatics: an algorithmic approach to sequence and structure
analysis by Ingvar Eidhammer, Inge Jonassen and William R. Taylor.
3. Essentials of Bioinformatics by Jin Xiong
CO-PO MAPPING:
Course Code: BTC601 Course Title: BIOINFORMATICS

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 3 2 1 1 1 3

CO 2 3 2 1 1 1 3

CO 3 3 3 2 2 2 2 1 1 3

Department of Computer Science and Engineering

Course Title of the course Program Core Total Number of contact hours Credit
Code (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
DATABASE
CSC631 MANAGEMENT PCR 3 0 0 3 3
SYSTEM
Pre-requisites 1. Computer fundamentals, Data structures.

2. Fundamentals of any computer programming languages.

Course Assessment methods [CA: 15%, MT: 25%, ET: 60%]

(Continuous (CT) and end
48
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

assessment (EA))

Course CO1: Understand the basic concepts and appreciate the applications of database
Outcomes systems
CO2. Comprehend the fundamentals of design principles for logical design of
relational
databases
CO3: Apply the query writing skill
CO4. Discuss the basic issues of transaction processing and concurrency control
Topics 1. Introduction of DBMS. 5L
Covered 2. Concept of E-R diagram, Extended E-R diagram.
5L
3. Relational Algebra
4L
4. Queries with various operations
4L
5. SQL Queries 4L
6. Index structure design
5L
7. Normalization (Different normal forms) 5L
8. Basic concepts on transaction processing 5L
9. Various concurrency-control protocols (2 phase locking, time stamp protocol)
5L

Text Books, Text Books:

and/or a. A. Silberschatz, H. F. Korth and S. Sudharshan, “Database System Concepts”,
reference Sixth Edition, Tata McGraw Hill, 2011.
material b. R. Elmasri, S. B. Navathe, “Fundamentals of DBMS Systems”, Pearson
education. Sixth Edition.
c. A. Kahate, “Introduction to Database Management Systems”, Pearson
Education, New Delhi, 2006.

Reference Books:

i. C.J.Date, A.Kannan and S.Swamynathan, “An Introduction to Database

Systems”, Eighth Edition, Pearson Education, 2006.

CO-PO MAPPING:

Course Code: CSC631 Course Title: DATABASE MANAGEMENT

SYSTEM

P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12

CO1 3 1 0 0 0 3 1 3 0 1 2 3

CO2 3 3 3 2 0 2 2 1 3 2 2 3

49
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO3 3 2 3 0 3 2 2 1 3 2 2 3

CO4 3 1 1 0 0 1 1 1 1 2 1 3

Department of Chemical Engineering

Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
CHC631 Process Control & PCR 2 1 0 3 3
Instrumentation

Mathematics, Unit Course Assessment methods (Continuous (CT) and end

Operations assessment (EA))
CT+EA
Course  CO1: Analyze open-loop system
Outcomes  CO2: Analyze and apply the knowledge of linear closed-loop systems.
 CO3: Develop working knowledge of control system by frequency response
 CO4: Analyze the response of instruments and ability to integrate knowledge
about instrument
 CO5:Explain the importance and application of instruments
Topics Laplace Transform, 1st order response, 1st order in series, linearization, 2nd order
Covered Dynamics (12)
Feedback control system, Servo and regulator problem, Transfer function of
Controller, Final control element, Control valve characteristics, Transportation Lag,
Routh-Hurwitz Criteria and stability (12)
frequency response of closed-loop, frequency response technique, Bode Diagram
and stability criteria (8)
Static and dynamic responses, Measurement of temperature and pressure (5)
instruments for process plant to measure flow, level and concentration of fluid (5)
Text Books, 1. Process Systems Analysis and Control, Donald Coughanowr McGraw-Hill
and/or Science/Engineering/Math; 2 edition (March 1, 1991)
reference 2. Chemical Process control, G. Stephanopoulos, PHI, 2008
material 3. Essentials of Process Control, Luyben et al. McGraw-Hill Companies (August
1, 1996)
4. Process control, Thomas Marlin, McGraw-Hill Education; 2nd International
edition (July 1, 2000)
5. Jone’s Instrumentation Technology (all the volumes)
6. Instrumentation and Devices by Rangan & Sharma
7. Considine’s Handbook on Instrumentation
8. Atomic absorption and Emission Spectrophotometers, Ed Metcalfe
9. Industrial Instrumentation, D.P.Eckman

50
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING:

Course Code: CHC631 Course Title: PROCESS CONTROL AND

INSTRUMENTATION

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 3 3 3 3 1 3 1 2 3
CO2 3 3 3 3 1 3 1 2 3

CO3 3 3 3 3 1 2 1 2 3

CO4 2 2 3 2 3 1 2 1 3 3

CO5 2 2 3 2 3 1 3 1 3 3

Department of Biotechnology

Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) /
Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTE610 Animal PEL 3 0 0 3 3

Biotechnology

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To elucidate the scope of Animal Biotechnology.

Outcomes
CO2: To learn the different areas of Animal Biotechnology applications.

CO3: To learn the basic technology in each area of Animal Biotechnology.

CO4: To learn the future prospect of the Animal Biotechnology.

Topics Animl Cell culture:History of animal cell culture and development, Development
Covered of primary culture, Development of cell line by enzymatic disaggregation, Culture
media and growth conditions. Cell type and characterization, origin of animal cell
line, maintenance and characterization of different cell lines, Marker gene

51
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

characterization (8)

Technology – Present and future :

Hybridoma technology/Monoclonal antibody technology, Vaccine production,

Organ culture, Transfection of animal cells, Future tissue engineering (4).

In Vitro Fertilization and Embryo Transfer:

Basic knowledge on Fertilization and embryology, Steps involved in IVF,

Fertilization by means of micro insemination, PZD, ICSI, SUZI, MESA (4)

Stem cells:

Classification and types, Sources, Markers, Differentiation signals, application,

IPSC, Cncer stem cells (4).

Gene Therapy:

Ex-vivo gene therapy, In vivo gene therapy, Viral gene delivery system,

Retrovirus vector system, Adenovirus vector system, Adeno-Associated virus

vector system, Herpex simplex virus vector system, Non-viral gene delivery system,
Prodrug activation therapy, Nucleic acid therapeutic agents (4)

Transgenic and Konck out Animals:

Methodology, Embryonic Stem Cell method, Microinjectionmethod, Retroviral

vector method, Applications of transgenic animals

Recombinanat protein expression and purification:

Expression vectors for mammalian proteins, Cell (S cerevicea, P pasturis etc.) for
large scale mammalian protein production, Post translational modification and
purification.

Text Books, 1. Animal Cell Culture by John R.W. Masters; Oxford University Press
and/or
2. Introduction to Cell and Tissue Culture by Jennie P. Mather and Penelope E.
reference
Roberts; Plenum Press, New York and London
material
3. Molecular Biotechnology: Primrose.

4. Animal Cell Biotechnology: R.E. Spier and J.B. Griffiths (1988), Academic
press.

5. Balasubramanian, Bryce, Dharmalingam, Green and Jayaraman (Eds.), Concepts

in Biotechnology, University Press, 1996

6. Hood L.E., Weissman I., Wood W.B. and Wilson J.H. Immunology, Benjamin

52
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Cummings, 1989

7. Biotol Series – Butterworth and Heineman, Oxford, 1992

CO-PO MAPPING:

Course Code: BTE610 Course Title: ANIMAL BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 1 1 1 2

CO2 1 1 1 3

CO3 2 1 2 2

CO4 1 1 1 2

Department of Biotechnology

Course Title of the Program Total Number of contact hours Credit

Code course Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTE611 Industrial PEL 2 1 0 3 3

Microbiology

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))

CT+EA

Course CO1: To interpretbasic concepts for the production of microbial

Outcomes products.fermentation and separation technology

CO2: To learn about the different types of Bioreators and their use.

CO3: To analyse the principles, and techniques for improving the yield and desired
properties in via strain improvement strategies.

CO4: They will be able to apply the knowledge related to processes, equipment for

53
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

industrial purpose and solve the problems.

Topics Industrial Microbiology– BTE611

Covered
Introduction to Fermentation Technology: 12

Basic idea on fermentation process, submerged, stationary, solid and semi-solid –

with their merits and demerits. Types of Media for Industrial fermentations; Media
Optimization; Sterilization of Industrial Media; Media sterilization,.Preparation of
microbial inoculum for Industrial fermentations.

Commercial strain development: 12

Induced mutations, Over producing decontrolled mutants, Catabolic derepressed
mutants; Genetically engineered strain; Protoplast fusion technique.
Improvement of strain by Site directed mutagenesis and Protein engineering :
Definition, methods and application. Improving microbial strain forproduction of
Amino acids Lysine and nucleosides and nucleotidesforaroma.Methods for
production of 5’ IMP and 5’GMP iii) Production of 5’IMP and 5’GMP by
fermentation.

Microbial processes for production of valuables 10

Introduction, on Microbial growth and its kinetics.Primary and secondary
metabolites and their regulation. Microbial production of organic acids,
antibiotics,alcohol, bakers yeast, Single cell protein (SCP); Vitamins.Organisms
used,(wild and mutated). production method- process, recovery of products
separation parameters , purification steps..Application .

Microbial Enzyme Technology: 10

Microbial process for production of enzymes.Commercial production of enzymes;
amylases, proteases,cellulase.Enzyme Modification - site
directedmutagenesis;Importance of Stability of enzymes;Enzyme stabilization by
selection and protein engineering for T4 Lysozyme;
Principles & techniques of immobilization of Enzymes, Application of immobilized
enzyme in Industrial processes

Text Books, Books

and/or
reference 1. Industrial Microbiology, Casida L E
2. Biotechnology: A textbook of industrial microbiology: CruegerW ,Crueger A
material
3. Industrial Microbiology, Prescott & Dunn

References:
1. Prescott’s and Dunn’s, A. Industrial Microbiology, 4 th edition.
CBS Publishers, New Dehli , India , 1987.
2. L.E. Cassida.Jr, Industrial Microbiology, New Age International Publisher
3. Atkinson.B and Marituna.F, Biochemical Engineering and Biotechnology
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Handbok, The Nature Press, Macmillan Publ. Ltd.

4. Bailey &Olis, Biochemical Engineering Fundamentals, MGH.
5. Review papers from reputed international journals to convey the current progress
.in this area.

CO-PO MAPPING:

Course Code: BTE611 Course Title: INDUSTRIAL

MICROBIOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 1 1 1
CO2 1 2
CO3 1 2 1
CO4 2 1 1 1

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTE612 NUTRACEUTICAL PER 3 0 0 3 3

AND
NUTRIGENOMICS

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To establish the correlation between nutraceuticals with cell signaling
Outcomes pathway.

CO2: To target nutraceuticals from different sources for prevention of disease.

CO3: To understand the interaction between gut microbiota with functional food
components and nutraceuticals and improvement of health.

CO4: To formulate the concept of nutrient gene interaction for prevention of

55
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

lifestyle related disorders.

Topics Nutraceuticals : General concepts of cell apoptosis/proliferation and molecular

Covered targets of nutraceuticals. [8]

Nutraceutical role in host immune response, in cancer, infection and chronic/acute

inflammations. Mechanism of action of Nutraceutical-signaling events, proteomics
and transcription factors. [8]

Nutraceuticals from food and herbs I: Polyphenols, flavonoids and other phenolic
compounds. [5]

Nutraceuticals from food and herb -II: Saponins, terpenoids and sulphur
compounds, Probiotic food with therapeutic applications, Prebiotics, Genomics of
Lactic Acid Bacteria [7]

Nutragenomics: An introduction, Nutrient gene interaction- Structure of nuclear

receptors with reference to carbohydrate, fat and vitamin A, Type 2 Diabetes
Mellitus and nutrigenomics, PPAR-γ and Diabetes Mellitus, Bioactive Peptides and
its role in Nutrigenomics [12]

Text Books, Books

and/or
reference 1. Nutritional Genomics: Discovering the Path to Personalized Nutrition by
James Kaput, Raymond L. Rodriguez, Wiley Functional Food Ingredients
material and Nutraceuticals by John Shi , CRC Press
2. Nutraceuticals by Lisa Rapport, Brian Lockwood , Pharmaceutical press

References:

1. Nutragenomics and Proteomics In Health Promotion and Disease Prevention

by Mohamed M. Rafi, FereidoonShahidi, CRC Press
2. Nutraceuticals: The Complete Encyclopedia of Supplements, Herbs,
Vitamins, and Healing Foods by Arthur J. Roberts, GenelleSubak-Sharpe,
Mary E. O'Brien (Designer) , Perigee Trade
3. Regulation of Functional Foods and Nutraceuticals: A Global Perspective
by Clare Hasler, Blackwell Publishing Professional

CO-PO MAPPING:

56
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course Code: BTE612 Course Title: NUTRACEUTICAL AND NEUTRIGENOMICS

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 3 3 3 2 3 3 2 2 1 1 1 1
CO2 3 3 3 3 3 3 3 3 1 1 1 3
CO3 3 3 3 3 3 3 3 1 1 1 1 3
CO4 3 3 2 3 3 3 3 1 1 1 1 3

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE613 Human PEL 3 0 0 3 3
Genomics
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
Cell Biology and Genetics CT+EA
(BTC301), Biochemistry
and Enzyme Technology
(BTC303), Molecular
Biology and rDNA
Technology (BTC401)
Course  CO1: To understand the general organization of human nuclear and
Outcomes mitochondrial genome and know about the salient features and characteristics.
 CO2: To acquire knowledge the human genome project and its implication on
clinical biology in the post genomic era.
 CO3: To familiarize with different scientific techniques used for studying
different features of genome.
 CO4: To get an overview about different applications of the genomic based
knowledge .
Topics 7. Patterns of genome organization (10)
Covered 8. Structural genomics (2)
9. Functional genomics (2)
10. Reverse genetics (2)
11. Gene patenting (2)
12. Electronic PCR (2)
13. Genome mapping and genome sequencing (2)
14. Specialized database in molecular biology (2)
15. Human genome project progress (2)
16. Genes in health and disease(2)
17. Genomic disorders and molecular medicine (2)
18. Minimal cell Genome (2)
19. Prospects of Gene therapy in Human (2)
20. Pharmacogenomics (2)
21. Genebank (2)

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

22. Legal status of gene bank (2)

Text Books, Textbook:

and/or
reference 1. T. A. Brown, Genomes, John Wiley & Sons
material
Reference Books
 Singer.M, and Berg.P, Genes and genomes, Blackwell Scientific Publication,
Oxford ,1991
 Beebe.T, and Burke.T, Gene Structure and Transcription, 2 nd edition,1992,
Oxford Univ Press
 Glick and Pasteurneck, Molecular Biotechnology, Principles and Applications of
Recombinant DNA technology, ASM Press
 Strachan & Reed, Human Molecular Genetics, Garland Science.
 Cantor & Smith, Genomics, John Wiley & Son

CO-PO MAPPING:

Course Code: BTE613 Course Title: HUMAN GENOMICS

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 3 2 2 1 1 3 1 2 1 2 1 3
CO 2 3 2 3 2 2 3 1 2 1 2 1 2
CO 3 3 3 3 3 3 3 1 2 1 2 1 3
CO 4 2 2 2 2 3 3 1 3 1 2 1 3

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE614 MOLECULAR PEL 3 0 0 3 3
VIROLOGY
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
Cell Biology (BTC 301/BT CT+EA
403), Molecular Biology
(BTC 401/ BT 404), and
Immunology (BTC 402/
58
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

BT 501)
Course  CO1: Acquire an understanding of virus life cycle and host-virus interactions.
Outcomes  CO2: Acquire an idea about detection, prevention and treatment of virus
infections.
 CO3: To learn about use of virus in biotechnology.
Topics Brief history and principles of virology. (1)
Covered Principles of virus classification. (2)
General structure of viruses; Viroids, Virusoids, Satellite viruses, and Prions. (2)
Genome of plant and animal viruses. Mobile genetic elements. (4)
Replications of RNA viruses. (5)
Replication of DNA viruses. (5)
Virus-cell interactions: cytopathology; virus entry and egress; host cell shut off and
IRES;viral persistence and latency. (6)
Methods to diagnose virus infections. (3)
Antiviral vaccines. (3)
Antivirals: interferons and its mechanisms of action. (2)
Gene silencing. (2)
Culture and purification of viruses. (2)
Viral vectors and gene therapy. (2)
New and emerging viruses (3)
Text Books, Text Books:
and/or 3. Principles of Virology: 4th Edition. By S. Jane Flint, Vincent R. Racaniello,
reference Glenn F. Rall, Anna Marie Skalka, and Lynn W. Enquist.
material Reference Books:
4. Fields Virology by Lippincott Williams and Wilkins.

CO-PO MAPPING:

Course Code: BTE614 Course Title: MOLECULAR VIROLOGY

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 2 1 1
CO2 2 1 1 1 1
CO3 2 1 2 2 1 1
Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE 615 BIOMETTALURGY PEL 3 0 0 3 3

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
Microbiology, Chemical Kinetics CT+EA
Course CO1: To recapitulate the basics of bioenergetics and to understand the relevant
Outcomes biogeochemistry & microbiology.
CO 2: To learn about the concepts of bioleaching and biobeneficiation along with the

59
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

microbiological aspects
CO 3: To learn about bioleaching processes with typical examples.
CO 4: To analyze the kinetics of bioleaching
CO 5: To understand the enzymatic mechanism of bioleaching.
Topics Recapitulation of basics of bioenergetics (ATP as an energy-rich molecule, oxidation-
Covered reduction reactions), Biogeochemical cycles – sulphur, iron, and manganese cycles.
Nature and characteristics of biogeochemically important micro-organisms. (9)

Bioleaching: definition, scope, advantages & disadvantages; Types: direct, indirect, &
indirect contact. Types of bioleaching with respect to reaction intermediates
(thiosulphate& polysulphide mechanisms). Autotrophs & heterotrophs as candidate
microorganisms for bioleaching. Bioleaching by aerobic and anaerobic
microorganisms. (9)

Bioleaching processes: in situ, heap & dump, & reactor bioleaching. Bioleaching of
copper by Acidithiobacillus from chalcopyrites, chalcocite, &covellite. Dump & heap
and reactor bioleaching of copper. Uranium bioleaching &biobeneficiation of gold.
Environmental pollution control in gold recovery processes. (9)
Kinetics of pyrite bioleaching – two-subprocess mechanism- ferric leach kinetics &
kinetics of bacterial oxidation of ferrous iron. Modelling of continuous tank
bioleaching of pyrite – unsegregated and segregated models. (9)

Oxidation of iron by Acidithiobacillus – enzymatic mechanism; role of cytochromes

&rusticyanin, elements of electron transport pathways in iron & sulphur oxidation.
(6)
Text Books, Text Books:
and/or 1. Pillai Abhilash, B. D. Pandey, K. A. Natarajan. Microbiology for Minerals,
reference Metals, Materials and the Environment, CRC Press, 2018
material 2. Ross W. Smith &ManoranjanMisra, ed. Mineral Bioprocessing, The Minerals,
Metals & Materials Society, 1991

Reference Books:
1. L. M. Prescott, J.P.Harley, D.A.Klein. Microbiology 5 thedn. Mc-Graw Hill,
2002.
2. M.E. Curtin, Microbial mining and metal recovery biotechnology (1), pp
229-235, 1983
3. Woods D, Rawling D.E., Bacterial bleaching and biomining in marx J.L. (ed),
Revolution in biotechnology, Cambridge University Press

CO-PO MAPPING:

Course Code: BTE615 Course Title: BIOMETTALURGY

PO1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12
CO1 - - - 1 - - 2 - - 2 - 1
CO2 1 - - 1 - 1- 3 1 - 2 - -
CO3 1 1 2 1 - 1 3 1 - 2 1 1
CO4 2 3 1 1 1 - - - - 1 - 1
CO5 1 2 1 3 - - - - - 2 - 2

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credi
Code Core Lectur Tutori Practic Total t
(PCR) / e (L) al (T) al (P) Hour
Electives s
(PEL)
BTE616 NANOBIOTECHNOLOG PEL 3 0 0 3 3
Y
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
BTC01 (Life Science), PHC01 CT+EA
(Physics), CYC01(Chemistry)
Course  CO1: Acquire an idea about nanoscale phenomenon
Outcomes  CO2: To learn about the basic investigation tools for the nanobiotechnology
 CO3: To learn about bottom up and top down synthesis of nanosystems
 CO4: to get comprehensive understanding of applications of nanotechnology
in biology
Topics Covered  Nanotechnology; introduction to miniaturization. (4)
 Iinvestigation tools: experimental methods and probes; basic principles of
scanning force microscopy; scanning electron microscopy; transmission
electron microscopy. Investigation tools: lithography (8)
 Nanomaterials: organic and inorganic nanoparticles. Synthesis, assembly, and
processing of nanostructures: phenomenon of self-assembly. (6)
 Molecular self-assembly and bottom up synthesis of nanomaterials. (6)
 Nanoparticles and cancer therapeutics; nanoparticle-based drug delivery. (6)
 Nanofiber-based scaffolds and tissue engineering; nanodiagnostics and
biosensing. (6)
 Nanotoxicology. (4)
 Future Concepts in Nanobiotechnology. (2)

Text Books, Text Book:

and/or 1. Understanding Nanomedicine - An Introductory Textbook by Rob Burgess.
reference
material Refrences Books
1. Springer Handbook of Nanotechnology, by Bharat Bhushan Springer
2. Nanobiotechnology: Concepts, Applications and Perspectives, by Christof M.
Niemeyer, Chad A. Mirkin, John wiley
3. Introduction to Nanotechnology, by Charles P. Poole, Frank J. Owens, Wiley-
Interscience
4. Nanofabrication and Biosystems : Integrating Materials Science, Engineering,
and Biology, by Harvey C. Hoch, Lynn W. Jelinski, Harold G. Craighead,
Cambridge University Press

CO-PO MAPPING:

Course Code: BTE616 Course Title: NANOBIOTECHNOLOGY

61
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12

CO 1 3 3 1 1 1 1 0 0 0 1 0 2

CO 2 3 3 2 3 3 1 0 0 0 1 0 2

CO 3 3 3 2 3 3 1 0 1 0 1 0 2

CO 4 3 3 2 3 3 3 1 1 0 1 0 2

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTE MARINE PEL 3 0 0 3 3
617 BIOTECHNOL
OGY
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
CT+EA
Course CO1: To learn about the bioprocess engineering aspects of marine products in
Outcomes commercial production
CO2: To learn about the industrial applications of various marine products and their
production
CO3: To study the specific applications in energy, pharmaceutical and environmental
sector.

Topics
Covered Bioprocess Marine microbiology 3
engineering of marine Photobioreactors – light regime
products mass transfer and scale up, downstream processing of 6
marine products
Management of Marine production, Storage and 4
transport.
Marine natural products, valuable chemicals, bioactive 4
compounds from micro-algae

Specialized aspects Cultivation of marine microorganism 3

marine biomedical and bioactive compounds from 3
marine organisms
commercial bio-products from marine organisms 2
biohydrogen production in photobioreactor, marine 3
enzymes
Marine bio-film and bio-remediation 3
marine bio-sensor and transgenic marine organisms 2
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Marine Pharmacology: Potentialities in the Treatment 3

of Infectious Diseases, Osteoporosis and Alzheimer´s
Disease
Molecular biodiversity 2
marine products as biomarkers 2
Economic and Regulatory Aspects of Marine 2
Biotechnology

Text Marine Bioprocess Engineering, J.G. Burgess R. Osinga R.H. Wijffels, Elsevier, 1999
Books,
and/or Handbook of Marine Biotechnology, KimSe-Kwon , Springer, 2015
reference
material

CO-PO MAPPING:

Course Code: BTE617 Course Title: MARINE BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 1 1 1 1 1 1 2

CO2 1 1 1 1 1 1 1 1 2 2

CO3 1 1 1 1 1 3 1 1 2 2

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE PROTEIN PEL 3 0 0 3 3
619 FOLDING,
MISFOLDING
AND
DISEASES

BTC401- Molecular Course Assessment methods (Continuous (CT) and end assessment
biology & rDNA (EA))
Technology; BTC 303
Biochemistry & Enzyme
Technology; BTC 301 Cell

63
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

biology and genetics

CT+EA
Course  CO1: To acquire an understanding of the protein structure
Outcomes  CO2: To learn about the principles of protein folding and misfolding
 CO3: To obtain a comprehensive idea of different diseases related to protein
misfolding
 CO4: Development of cumulative understanding of protein folding, misfolding
and diseases to find much-needed cure for the relevant conditions.
Topics Basic of protein misfolding related diseases. The hierarchical structure of the protein.
Covered Principles of protein stability and folding. (16)
Protein misfolding and aggregation. Protein quality control: molecular chaperones,
protein degradation, autophagy and aging. (12)
Prion Diseases. Alzheimer’s Disease. Parkinson’s Disease. Huntington’s Disease and
other unstable repeat disorders. Amyotrophic lateral sclerosis and frontotemporal
lobar degeneration. (14)
Text Text Books:
Books, 1. Fundamentals of Neurodegeneration and Protein Misfolding Disorders by Martin
and/or Beckerman, Springer
reference 2. Introduction to Protein Structure by Carl IV Branden, Routledge
material 3. Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and
Protein Folding by Alan Fersht, W. H. Freeman.

CO-PO MAPPING :

Course Code: BTE619 Course Title: PROTEIN FOLDING, MISFOLDING AND

DISEASES
PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12

CO 1 1 3 3 3 2 1 1 0 3 3 1 3

CO 2 1 3 3 3 2 1 1 0 3 3 1 3

CO 3 1 3 3 3 2 1 1 0 3 3 1 2

CO 4 3 3 3 3 2 1 1 0 3 3 1 3

64
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) /
Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTE620 ENGINEERING PEL 3 0 0 3 3

RESISTANCE
IN PLANTS

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

BTC502 (Cell & Tissue CT+EA

Culture of Animals & Plants)

Course CO1: To develop the basic knowledge for genetic improvement of crop plants.
Outcomes
CO2: Understanding the sources of useful genes required for engineering
resistance.

CO3: Learning of fundamentals of gene mapping and gene isolation.

CO4: Learning the basics and methods of genetic transformation of plants.

CO5: Solving problems related to biotic and abiotic stress faced by crop plants.

Topics Introduction: Principles of gene manipulation in plants and basic concepts of

Covered genetic improvement of crop plants[5]

Molecular markers & Cloning genes:Identifying the good gene sources, general
strategies for cloning genes from plants, Cloning methods based on DNA
insertions, subtractive cloning, map-based cloning, chromosome walking,
chromosome jumping, morphological markers, biochemical markers, molecular
markers – RFLP, RAPD, AFLP, ISSR, RAMP, STMs, fingerprinting, SNPs[10]

Genetic Engineering:Agrobacterium-plant interaction; virulence; Ti and

Riplasmids; opines and their significance; T-DNA transfer; disarmed Ti
plasmid;Genetic transformation Agrobacterium-mediated gene delivery; cointegrate
andbinary vectors and their utility; direct gene transfer - PEG-
mediated,electroporation, particle bombardment and alternative methods;
screenable andselectable markers; characterization of transgenics; chloroplast
transformation [10]

65
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Applications:Genetic engineering of resistance to biotic stress, tolerance to abiotic

stress, removal of environmental pollutants, quality nutrition and health, molecular
farming[10]

Biosafety concerns:Removal of selectable markers from GM crops,Modern tools

of genetic manipulation of plants; genome editing[7]

Text Books, Text Books:

and/or 1. H.S.Chawla, Introduction to Plant Biotechnology, Oxford & IBH Publishing
reference co. Pvt..Ltd
2. Slater.A.,NigelW.S,Flower.R.Mark , Plant Biotechnology: The Genetic
material
Manipulation of Plants, 2003, Oxford Univesity Press.
3. Plant Pathology; Fifth Edition, Elsevier; By Geroge N. Agrios.
4. Primrose, S. B., & Twyman, R. M. (2006). Principles of Gene Manipulation
and
Genomics. Malden, MA: Blackwell Pub.

Reference Book:
1. Plant Immunity; Methods in Molecular Biology, 2011, 712, Springer.
2. Buchaman, Gursam, Jones, Biochemistry and Molecular Biology of Plants,
1ed, 2000, L.K.International.
3. Bhojwani and Razdan –Plant Tissue Culture: Theory and Practice 1996
Elsevier

CO-PO MAPPING:

Course Code: BTE620 Course Title: ENGINEERING

RESISTANCE IN PLANTS
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO1 PO1 PO1
0 1 2

CO1 2 0 0 1 0 0 2 0 1 0 0 2

CO2 1 0 0 2 0 0 2 0 2 0 0 1

CO3 1 0 0 2 2 3 2 2 2 0 0 1

CO4 3 0 0 2 2 2 2 3 3 0 0 3

66
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO5 3 2 3 2 2 2 3 3 2 0 0 3

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Cre
Code Core dit
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hour
(PEL) s

BTS651 MOLECULAR PCR 0 0 3 3 1.5

BIOLOGY AND
rDNA
TECHNOLOGY
LABORATORY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To understand the principle of isolation of nucleic acids through different
Outcomes techniques.

CO2: To understand the techniques used in manipulation of nucleic acids.

CO3: To develop expertise to apply the toolsof gene cloning and solve the
problems associated with production of recombinant protein from genetically
modified microorganisms.

CO4: To develop an idea for proper documentation of the work including

laboratory procedures, experimental conditions, materials used, equipment used
and the results

CO5: To understand the basic hazards of working with nucleic acids and safety
measures.

Topics 1. Isolation of genomic DNA

Covered
2. Quantification of DNA

3. Agarose Gel Electrophoresis of DNA

4. Isolation of RNA

67
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

5. Agarose Gel Electrophoresis of RNA

6. Isolation of plasmid – agarose gel electrophoresis (quantitation and purity

test)

7. Restriction digestion of plasmid – agarose gel electrophoresis

8. Bacterial transformation using plasmid having antibiotic resistant marker and

some other genetic markers.

9. Southern Blotting

10. PCR technique

Text Books, Sambrook et al., “Molecular Cloning” A Laboratory Manual

and/or
reference
material

CO-PO MAPPING:

Course Code: BTS651 Course Title: MOLECULAR BIOLOGY

AND rDNA TECHNOLOGY
LABORATORY
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO1 PO1 PO1
0 1 2
CO1 2 2 2 1 2
CO2 1 2 2 1 2
CO3 2 2 2 2 1 2
CO4 1 3 2
CO5 2 2 2

Department of Biotechnology
Course Title of the courseProgram Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTS652 BIOINFORMATICS PCR 0 0 3 3 3
LABORATORY
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
Programming and Data Structure CT+EA
(CSC431)

68
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course  CO1: To acquire programming knowledge to analyze biological data

Outcomes  CO2: To learn about different biological databases and retrieval of biological
data in different file formats.
 CO3: To learn different bioinformatics softwares related to sequence,
structure and phylogeny
Topics 23. Bash programming (Linux commands) for data mining (3)
Covered 24. Handling Biological databases and sequence and structure retrieval (2)
25. Pairwise Sequence Alignment: BLAST tool and interpreting the results
(1)
26. Multiple Sequence Alignment: Clustal, Muscle etc. (1)
27. Phylogenetics methods for phylogenetic tree constructions: Mega, Phylip
(1)
28. C and Python scripts to analyse and interpret biological data (3)
29. Protein Structure and its visualization, structural alignment softwares:
PyMOL, Rasmol, VMD (1)
30. Protein Structure prediction softwares: Modeller, I-Tasser, Psipred (1)
31. RNA related softwares: Vienna Package (1)

Text Books, Text Books:

and/or 4. The Linux Command Line: A Complete Introduction 1st Edition by William
reference E. Shotts Jr.
material 5. Python Crash Course by Eric Matthews
Reference Books:
5. A Byte of Python by C.H. Swaroop
6. A Practical Guide to Linux Commands, Editors and Shell Programming 3rd
Edition by Mark G. Sobell

CO-PO MAPPING:

Course Code: BTS652 Course Title: BIOINFORMATICS

LABORATORY
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO1 PO1 PO1
0 1 2

CO 1 3 3 1 3 3 2 3

CO 2 3 2 1 3 2 3 3

CO 3 3 2 2 3 3 3 3 1 2 3

Department of Computer Science and Engineering

Course Title of the course Program Core Total Number of contact hours Credit
Code (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
69
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

DATABASE
MANAGEMENT
CSS681 PCR 0 3 3 1.5
SYSTEM
LABORATORY
Pre-requisites 1. Computer fundamentals, Data structures

2. Fundamentals of any computer programming languages

Course Assessment methods CT+EA (Class test, Viva, Assignments, Lab test)
(Continuous (CT) and end
assessment (EA))
Course CO1: Understand, appreciate and effectively explain the underlying concepts of
Outcomes database technologies
CO2. Design and implement a database schema for a given problem
CO3. Populate and query a database using SQL DML/DDL commands
Topics 1. SQL Queries
Covered 2. PL/SQL assignments

Text Books, Text Books:

and/or SQL and PL/SQL by Evan Bayross.
reference
material

CO-PO MAPPING:

Course Code: CSS681 Course Title: DATABASE MANAGEMENT

SYSTEM LABORATORY
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO1 PO1 PO1
0 1 2

CO1: 3 3 3 2 1 2 0 1 2 2 3

CO2. 3 3 3 1 1 2 0 2 2 2 2

CO3. 3 3 3 2 1 2 0 2 2 2 2

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

70
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

PRINCIPLES
MSC731
OF PCR 3 0 0 3 3
MANAGEMENT
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))

CT+EA

Course • CO1:To make budding engineers aware of various management functions

Outcomes required for any organization
• CO2:To impart knowledge on various tools and techniques applied by the
executives of an organization
• CO3:To make potential engineers aware of managerial function so that it
would help for their professional career
• CO4:To impart knowledge on organizational activities operational and
strategic both in nature
• C05: To impart knowledge on each functional area of management like
Marketing, Finance, Behavioral Science and Quantitative Techniques and
decision science

Topics UNIT I: Management Functions and Business Environment: Business

Covered environment- macro, Business environment -micro; Porter’s five forces,
Management functions –overview, Different levels and roles of management,
Planning- Steps, Planning and environmental analysis with SWOT, Application
of BCG matrix in organization(8)
UNIT II: Quantitative tools and techniques used in management: Forecasting
techniques, Decision analysis, PERT & CPM as controlling technique (7)
UNIT III: Creating and delivering superior customer value:Basic understanding
of marketing, Consumer behavior-fundamentals, Segmentation, Targeting &
Positioning, Product Life cycle. (8)
UNIT IV: Behavioral management of individual: Motivation, Leadership,
Perception, Learning. (8)
UNIT V: Finance and Accounting: Basics of Financial management of an
organization, Preparation of Final Accounts, Analysis of Financial statements,
Cost Volume Profit (CVP) Analysis, An overview of financial market with special
reference to India. (12)

Text Books, Text Books:

and/or 1. Financial Management, 11th Edition, I M Pandey, Vikas Publishing
reference House.
material
2. Marketing Management 15th Edition, Philip Kotler and Kelvin Keller,
Pearson India
3. Management Principles, Processes and practice, first edition, Anil Bhat
71
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

and Arya Kumar, Oxford Higher education

4. Organizational Behavior,13 th edition, Stephen P Robbins, Pearson
Prentice hall India
5. Operations Management, 7th edition (Quality control, Forecasting), Buffa
& Sarin, Willey

CO-PO MAPPING:

Course Code: MS631 Course Title: PRINCIPLES OF

MANAGEMENT
COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO1 PO1 PO1
0 1 2

CO1
3 2 2
CO2
2 2 2
CO3
2 3 2
CO4
1 3
CO5
2 2 2 2

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P)# Hours
(PEL)
BTE710 MOLECULAR PEL 3 0 0 3 3
PLANT
PATHOLOGY
Pre-requisites Course Assessment methods (Continuous evaluation (CE) and end
assessment (EA))
BTC01 CE+EA

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course CO1: To understand molecular mechanisms of plant defense systems.

Outcomes CO2: To understand molecular mechanisms of pathogenesis.
CO3: To have the idea to design strategies for protection of plants.
Topics Introduction to molecular plant pathology [1]
Covered Plant diseases [2]
Plant disease development and environment [2]
Effects of pathogen on plant physiology [2]
Biochemistry of plant defense reactions [5]
Plant-pathogen interactions [5]
Genetic regulation of resistance in host plants [5]
Genetic regulation of virulence in pathogen [5]
Mechanisms of host defense [5]
Mechanisms of pathogenesis [5]
Biotechnological approach for plant protection; genetically modified plants to
protect against pathogens [5]
Text Books, Text Book:
and/or 1. Plant Pathology; Fifth Edition, Elsevier; By Geroge N. Agrios.
reference 2. Biochemistry and Molecular Biology of Plants; American Society of Plant
material Biologists; By Bob Buchanon, Wilhelm Gruissem and Russel Jones.
3. Plant Immunity; Methods in Molecular Biology, 2011, 712, Springer.
4. Plant-Pathogen Interactions; Methods in Molecular Biology; By Pamela
Ronald, 2007, 354, Springer.
5. Plant-Pathogen Interactions; Annual Plant Reviews; By Nick Talbot, 2004,
11, Blackwell Publishing.

CO-PO MAPPING:

Course Code: BTE710 Course Title: MOLECULAR PLANT

PATHOLOGY
COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 1 2 1 2 1 2 1 1 1

CO2 1 1 1 2 1 1 1

CO3 1 1 2 2 2 2 1 1 2 1 1

Department of Biotechnology
Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTE CANCER PEL 3 0 0 3 3
711 BIOLOGY
AND CELL
SIGNALING
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))

73
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

BTC301-Cell Biology and CT+EA

Genetics/BT-817- Cancer
Biology
Course  CO1: To understand the basic concepts of cancer biology and related cellular
Outcomes signaling
 CO2: To understand the development and causes of cancer.
 CO3: To understand the therapeutic aspects of cancer prevention
 CO4: To identify the target molecules that are associated with cancer so that the
cancer preventive small molecule inhibitors/phytochemicals can be screened.
Topics Cancer Biology
Covered
Introduction to Cancer and Molecular basis of cancer [2]
Mutation and DNA damage repair mechanism [2]
Cell cycle [3]
Oncogenes (tumor viruses) , Tumor suppressors [3]
Epigenetics, non-coding RNAs and genome fluidity in cancer [4]
Cancer and Stem Cells, Angiogenesis, Apoptosis [4]

Cancer therapy, Future of Cancer research [3]

Cell Signaling related to cancer

Introduction to cellular signaling [3]

Signaling molecules – (e.g. Hormones, Interferons and others) [3]
Receptor-mediated signaling in cells [3]
Role of different transcription factors and kinases (e.g. MAP kinases and other ser/thr
kinases) [4]
Involvement of different signal transduction pathways during cancer initiation,
progression and metastasis [5]
Small molecule inhibitors of cancer [3]

Text Text Books:

Books,
and/or 1. Weinberg RA. The Biology of Cancer, 2nd Edition. Garland Science, 2013.
reference 2. Cellular signal processing , 2nd Edition by Friedrich Marks, Ursula Klingmuller
material and Karin Muller-Decker, Garland Science

Reference: Selected reviews and primary scientific literature

CO-PO MAPPING:

74
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course Code: BTE711 Course Title: CANCER BIOLOGY

PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12

CO 1 1 - 2 2 - 1 - - 1 2 1 2

CO 2 1 1 2 2 1 1 1 1 2 2 1 2

CO 3 1 1 1 2 1 - 1 - 1 2 1 2

CO 4 1 1 2 2 1 2 3 - 1 1 1 2

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core
(PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)

BTE712 FOOD PER 3 0 0 3 3

BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))

CT+EA

Course CO1: To quantitate and identify the spoilage microorganisms present in food.
Outcomes CO2: To learn the concepts of food fermentation and increase the shelf life of food.
CO3: To learn the concepts in genetically modified food and increase the agricultural
yield by using genetic engineering approach.
CO4: To apply the concepts of antioxidant and nutraceutical for health and wellness.
CO5: To follow the regulations and ethical issues of food safety by using good
manufacturing practices in industry andgenetically modified food.

Topics Food for health and wellness [2]

Covered
Food Microbiology: [6]

Detection of microorganism in food – role of PCR, DNA CHIP, rapid methods for
identification of microorganism in food, immunological methods, Bioassay,

75
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Biosensors- detection of toxin, heavy metal , pesticide and herbicides

Food preservation [10]

Pasteurization, sterilization, Canning, Irradiation, Dehydration, low temperature

Food preservation, use of preservatives,

Food fermentation [8]

Role of lactic acid bacteria in fermentation and strain improvement, Fermentation

of meat, fish, vegetables, beverages , dairy product, non beverage product , use of
genetic engineering techniques for improved quality product.
Genetically modified food [6]

Fruit ripening, improvement of sweetness, flavor, starch, amino acid, vitamin

content, Golden rice. Safety aspects of genetically modified food, Single cell
protein, single cell oil, Spirulina,

Biotechnology in relation to food product and Food Safety (5+5)

Antioxidant, nutraceutical, Nutrigenomics

Legal status of irradiated food and preservatives, Concept of HACCP, Hazop,

codex alimentarius, ISO series

Text Books, Text Book

and/or
reference Food microbiology by James . M. Jay
material Food Microbiology by Frazier and Westhoff

Plant Biotechnology by Slater

Reference Book

Fundamentals of Food Biotechnology by Lee

CO-PO MAPPING

Course Code: BTE712 Course Title: FOOD BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 3 3 3 3 3 3 3 2 1 1 2 3
CO2 3 3 3 3 2 2 3 2 1 1 2 3
CO3 3 3 3 3 3 3 3 3 2 1 2 3
CO4 3 2 3 3 1 3 3 2 2 1 1 3
CO5 3 2 2 2 3 3 3 3 3 3 3 3

76
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology
Course Title of the course
Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE713 BIOPHARMACEUTICAL PEL 3 0 0 3 3
PROCESS DESIGN

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
CT+EA
Course CO1: To learn about the manufacturing process and facility design for
Outcomes biopharmaceutical products
CO2: To acquire knowledge of detailed design of GMP compliant biopharma plant
CO3: To study the design and optimization of downstream processes of therapeutic
protein manufacture in a commercial set up
CO4: To learn about technology transfer, regulation, validation and quality
assurance of biopharma industry
Topics Manufacturing process - Drug substance manufacturing, drug product manufacturing,
Covered key factors for process evaluation. Manufacturing and storage of cell bank.
Comparison of batch and continuous process for fermentation. Difference between
suspension fermenters for cell culture and microbial fermentation. [6]

Design and construction of manufacturing facilities for mammalian cell derived

pharmaceuticals. Detailed design of a GMP compliant plant with process flow
diagram along with utilities, water treatment, waste management and location
selection [6]

Downstream processing - Harvest of therapeutic proteins from high cell density

fermentation broths – centrifugation and filtration. Expanded bed adsorption for
separating the biopharmaceutical product from crude solution. Ultrafiltration process
design and implementation for biopharmaceutical product recovery. Virus filtration
process design for biopharmaceutical product recovery. Product recovery
of biopharmaceutical products from transgenic sources – aqueous two phase
extraction [12]

Role of process development group and manufacturing group in biopharmaceutical

process start up. [3]

Making changes to a biopharmaceutical manufacturing process during development

and commercial manufacturing – a case study [2]

Biosimilars and non-innovator biotherapeutics in India – an overview of current

situation [2]

77
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Fundamental of Quality assurance, Structure of Quality Management Systems,

Responsibility of Management and Training of Personnel, Quality Assurance in
Development. [5]

Quality assurance in manufacturing, GMP, Process validation for cell culture derived
pharmaceutical proteins. Regulation [6]

Text Books, Books

and/or
reference Text
material
1. Process Scale Bioseparations for the Biopharmaceutical Industry, Abhinav A.
Shukla, Mark R. Etzel, ShishirGadam, CRC Press
2. Manufacturing of Pharmaceutical Proteins, Stefan Behme, Wiley-VCH
References

1. Pharmaceutical Production Facilities: Design and Applications, Graham

Cole, Informa Healthcare
2. Large-scale Mammalian Cell Culture Technology, Lubiniecki, CRC Press

CO-PO MAPPING

Course Code: BTE713 Course Title: BIOPHARMACEUTICAL

PROCESS DESIGN
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 3 1 1 1 2 1 1 1 1 2

CO2 2 2 3 1 1 1 2 1 1 1 1 2

CO3 2 2 2 1 1 1 1 1 1 1 1 2

CO4 2 2 2 1 1 1 2 2 1 1 3 2

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE714 BIOENERGY PEL 3 0 0 3 3

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
CT+EA
78
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course 1. Learn about energy crisis, problems of fossil fuel use, global warming
Outcomes 2. Learn about production of biological solid fuel.
3. Learn about gaseous biofuel production like methane and hydrogen in detail.
4. Learn about liquid biofuels
5. Learn about benefits and deficiencies of biofuels, life cycle analysis

Topics Energy and fossil fuel use – fossil fuel use, fossil fuel reserves, sustainable fuel sources
Covered [4]

Consequences of burning fossil fuel – effects of industrial (anthropogenic) activity on

greenhouse gases, sources of greenhouse gases
[3]

Mitigation of global warming – Kyoto protocol, reduction in global greenhouse gases,

fuel cells, sequestration of carbon dioxide, alternative energy sources, energy storage.
[4]

Biological solid fuels – 1st, 2nd and 3rd generation biofuels, types of biomass available,
energy and fuel generation using biomass.
[5]

Gaseous biofuels – methane production using anaerobic digestion process, sewage

sludge and from landfill sites, use of methane as transport fuel. Hydrogen production
from biological material, biological production of hydrogen, photosynthetic hydrogen
production, hydrogen storage, use as transport fuel. Diethyl ether production
[6]

Liquid biofuels to replace petrol – methanol production. Large scale ethanol production
from biomass, use of lignocellulosics for ethanol production, ethanol extraction after
production, use of ethanol as fuel. Butanol production and use.
[6]

Liquid biofuel to replace diesel – synthetic diesel (FT synthesis), bio-oil (pyrolysis),
microalgal biodiesel, biodiesel from plant oils and animal fats, properties of biodiesel,
glycerol utilization.
[5]

The benefits and deficiencies of biofuels – reduction in fossil fuel use, fuel economy,
reduction in carbon dioxide emission from biofuels, improvement in biodiesel quantity
and quality, life cycle analysis of biofuels.
[6]

Jatropha cultivation, National hydrogen energy road map.

[3]

Text Books, Books.

and/or 1. Biofuels production, application and development. Alan Scragg, CABI.
reference
material

79
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING :
Course Code: BTE714 Course Title: BIOENERGY
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 1 1 2 3 1 1 1 2

CO2 2 2 2 2 3 1 1 1 2

CO3 2 2 2 2 3 1 1 1 2

CO4 2 2 2 2 3 1 1 1 2

CO5 1 1 2 3 1 1 1 2

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE715 PROJECT PEL 3 0 0 3 3
ENGINEERING
FOR
BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
CT+EA
Course CO1: To learn about detailed design of a manufacturing plant
Outcomes CO2: To learn about cleaning, sterilization, waste management and utilities of a
biotechnology production plant
CO3: To study about Planning, construction and commissioning of a
biopharmaceutical manufacturing plant
CO4: To learn about project management and financial aspects of the plant
Topics Introduction Basic considerations in plant design, project identification, preliminary
Covered techno-economic feasibility. Process flow Diagrams and symbols: Symbols of Process
Equipments& their concepts, types of flow diagrams, Importance of Laboratory
development, pilot plant, scale up methods [6]

Piping and valves for biotechnology: design, piping materials, polishing, passivation,
sizing of pipes and tubes, connections and cleanability, piping applications, supporting
and insulating sanitary tubing, in-line instruments, hoses, valves. [6]

Cleaning of process equipment: design and practice, sterilization of process equipment,

pharmaceutical water systems: design and validation, utilities for biotechnology
production plant, biowaste decontamination systems, Heating, ventilating & air
conditioning (HVAC) [6]

80
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Programming & facility design, project planning, containment regulations affecting the
design and operation of biopharmaceutical facilities. [6]

Planning, construction and commissioning of a biopharmaceutical manufacturing

plant: planning, construction, commissioning, qualification, validation, project
schedules, cost estimates, organization of an engineering project, role & selection of
contractors, legal aspects of facility engineering, health, safety and environmental law,
building law. [6]

Product sales and manufacturing costs: basic principles of cost calculation, fixed cost,
variable cost, depreciation, interest, typical costs of biotechnological manufacturing
processes, profit and loss calculation. [6]

Investments: investment targets, types of investments, investment appraisal, cost

comparison, profit comparison, internal rate of return, dynamic payback time. [3]

Production concepts: capacity planning, dilemma of in-house manufacturing, aspects

of manufacturing out-sourcing, contractual agreements, technology transfer, process
optimization after market launch, supply chain management. [3]

Text Books, Text Books:

and/or
reference 1. Bioprocess engineering: system, equipment and facilities, B K Lydersen,
material NAD’Elia, K M Nelson. Wiley
2. Manufacturing of pharmaceutical proteins, Stefan Behme, Wiley

Reference Books:

1. Plant design and Economics for chemical engineers, peter M. S. Timmerhaus, K. D.

McGraw Hill.

2. Project Engineering with CPM and PERT, Modes J. Philips, Rheinhold publishers.

CO-PO MAPPING :
Course Code: BTE715 Course Title: PROJECT ENGINEERING FOR
BIOTECHNOLOGY
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 2 1 1 2 1 1 1 1 2

CO2 3 3 3 2 1 1 3 1 1 1 1 2

CO3 3 3 3 2 1 1 2 1 1 1 1 2

CO4 3 3 3 2 1 1 2 1 1 1 3 2

81
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology
Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTE STRUCTURAL PEL 3 0 0 3 3
716 BIOLOGY

BTC401- Molecular Course Assessment methods (Continuous (CT) and end assessment
biology & rDNA (EA))
Technology and BT C303
Biochemistry & Enzyme
Technology
CT+EA
Course  CO1: To acquire understanding of the basic building blocks of life
Outcomes  CO2: To learn about the most common structural motifs found in protein and DNA
 CO3: To understand the atomic level interaction between the protein and DNA
 CO4: To learn how to determine protein structure
Topics Basic structural principles - The building blocks, motifs of protein structure, alpha-
Covered domain structures, alpha/beta structures, beta structures, folding and flexibility, DNA
structures. (8)
Structure, function and engineering - DNA recognition in prokaryotes by helix-turn-
helix motifs. (4)
DNA recognition by eukaryotic transcription factors, specific transcription factors (5)
Enzyme catalysis with example of serine proteinases, membrane proteins, signal
transduction, fibrous proteins (7)
Recognition of foreign molecules by immune system, structure of spherical viruses (8)
Prediction, engineering and design of protein structures, determination of protein
structures (10)
Text Text Book:
Books, 1. Introduction to Protein Structure: Second Edition by Carl IV Branden, Routledge
and/or
reference Reference book:
material 1. Structure and Mechanism in Protein Science A Guide to Enzyme Catalysis and
Protein Folding: Alan Fersht

CO-PO MAPPING :

Course Code: BTE716 Course Title: STRUCTURAL BIOLOGY

PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12

CO 1 1 3 3 3 0 1 1 0 1 2 0 1

CO 2 1 3 3 3 0 1 1 0 1 2 0 1

82
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO 3 1 3 3 3 0 1 1 0 1 2 0 1

CO 4 3 3 3 3 3 0 0 0 1 2 0 3

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE717 ENVIRONMENTAL PEL 3 0 0 3 3
BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
CT+EA
Course CO1: To learn about air pollution monitoring and control
Outcomes CO2: To learn about waste water treatment processes along with analytical
procedures
CO3: To study about solid waste management
CO4: To acquire knowledge on bioremediation of pollutants
Topics Air pollution control methods and equipment - Primary and secondary air pollutants,
Covered Effect of air pollutants on health, Control of gaseous and particulate pollutants, air
pollution control equipments. 6

Water pollution: sampling and analysis - Sampling, BOD and COD analysis,
Bacteriological measurements, Numerical problems 5

Water and waste water treatment processes - Overview of treatment principles.

Primary treatment – screening, sedimentation, flotation, neutralization etc. 4

Secondary treatment - Activated sludge process, extended aeration, Trickling filter,

Aerated lagoons, Waste stabilization ponds, Aquatic plant systems, UASB reactors.
Design of a complete mix activated sludge process. 8

Biomethanation. Nitrification and denitrification operations. Phosphorus removal.

Sludge treatment and disposal. Tertiary treatment. Membrane based treatment
processes. 8

Solid waste management, Vermiculture, hazardous waste management 5

Specialized aspects - Bioremediation for recovery of metals, Xenobiotics, Degradation

of chlorinated hydrocarbons, polyaromatic hydrocarbons, Phytoremediation. Reactors
in bioremediation. 6

83
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Text Books, Books

and/or
reference Text
material 1. Introduction to waste water treatment processes, Ramalho, Elsevier.
2. Environmental Engineering: A design Approach, Sincero, Arcadio. P, Sr.
&Greogia; PHI
3. Waste water treatment and disposal, Arceivala, Wiley
4. Environmental Biotechnology, Alan Scragg, Oxford University press

Reference
1. Waste water Engineering: Treatment, disposal, reuse, by Metcalf & Eddy, Tata
Mc Graw Hill
2. Industrial Water Pollution Control, Eckenfelder, McGraw Hill.

CO-PO MAPPING :

Course Code: BTE717 Course Title: ENVIRONMENTAL

BIOTECHNOLOGY
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 2 1 1 1 3 1 1 1 2

CO2 3 2 2 1 1 1 3 1 1 1 2

CO3 3 2 2 1 1 1 3 1 1 1 2

CO4 3 2 2 1 1 1 3 1 1 1 2

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE718 PROTEOMICS PEL 3 0 0 3 3
AND PROTEIN
ENGINEERING

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
BTC303 Biochemistry and CT+EA

84
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Enzyme Technology;
BTC401 Molecular Biology
and Recombinant DNA
Technology;
Course CO1:Students will acquire knowledge on protein structure and function and will be
Outcomes able to apply the understanding in designing strategies for proteomic analysis and
protein engineering.
CO2:Students will be acquainted with tools and techniques for proteomic analysis and
will be able to analyze proteomic data using databases.
CO3: Students will be acquainted with tools and techniques for protein engineering and
will be able to apply them to solve problem related to protein function and efficiency.
Topics 1. Introduction to proteinstructure and function: Elementary ideas of bonding and
Covered structure, stereochemistry; spectroscopic techniques. Amino acid structure and
properties to 3D structure of protein. Basic principles of protein folding
anddynamics. Protein sequence andevolution. [10]
2. Proteomics and its application: Chromatography principles. Analytical protein
and peptide Separation, Protein Digestion Techniques, Mass Spectrometers for
protein and peptide analysis, protein identification by peptide Mass fingerprinting.
Mining proteomes, protein expression profiling, identifying protein-protein
interactions and protein complexes, Mapping protein modifications. [16]
3. Protein Engineering: Proteins design and engineering, Random, site directed
mutagenesis; Strategies to alter catalytic efficiency; structure prediction and
modeling proteins; Molecular graphics in protein engineering; Dynamics and
mechanics; Drug-protein interactions and Design; applications of engineered
proteins. [16]
Text Books, Textbooks:
and/or 1. R.M. Twyman; Principles of Proteomics, Bioscientific Publishers.
reference 2. Biotechnology, 2nd Edition 2015. David Clark and Nanette Pazdernik. Academic
material Cell.
Reference Books:
1. B.Alberts,D.Bray, J.Lewis et al, Molecular Biology of the Cell, Garland Pub. N.Y
1983.
2. Richard J. Simpson, Proteins and Proteomics, I.K. International Pvt Ltd.
3. Daniel C. Liebler, Introduction to Proteomics: Tools for the New Biology, Humana
Press.
CO-PO MAPPING:

Course Code: BTE718 Course Title: PROTEOMICS AND PROTEIN

ENGINEERING
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 2 1
CO 2 2 2 2 1 1 1 1
CO 3 2 2 2 1 1 1 1 1

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE719 MOLECULAR PEL 3 0 0 3 3
MODELLING
& DRUG
DESIGN

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
Biochemistry and Enzyme CT+EA
Technology, Bioinformatics
Course  CO1: To understand the physical basis of the structure, the dynamic evolution of
Outcomes the system, and the function of biological macromolecules.
 CO2: To learn the fundamental concepts of structure-activity relationships
 CO3: To learn design of novel, biologically active compounds and Toelucidate
the mechanism of action of drugs
Topics 2. Introduction to molecular Simulation Techniques (5)
Covered 3. Quantum chemistry for Modeling of small molecules (5)
4. Molecular Dynamics Methods- Molecular Dynamics of rigid non linear poly
atomic molecules in ensembles, Structural information from M.D. (5)
5. Force fields for molecular modeling: Choice of functional form. Parametrization
of a force field, Distributed multipole and polarizable forcefields, Hydrophobic
effect and solvation energy. Potentials of mean force. (10)
6. Conformational analysis: Geometry optimization using steepest descent and
conjugate gradients. Restrained and constrained molecular dynamics. Distance
geometry. Case studies: Prediction of protein-protein interactions. DNA
conformation. (10)
Principles of ligand based drug design: SAR, QSAR and 3D-QSAR. Receptor based
drug design: Principles of receptor based de novo ligand design. Rigid body molecular
Docking. (7)
Text Books, Text Books:
and/or 1. A R Leach-Molecular Modelling,. Principles and application 2nd edition–
reference Prentice Hall.
material 2. Krogsgaard, L-Text Book of Drug Design and Discovery-2002, Taylor and
Francis, London

Reference Books:
1. G.Walsh-Biopharmaceuticals-Biochemistry and Biotechnology-2003, Wiley
2. Scolnick.J.(2001) Drug Discovery and Design .Academic Press, London
N. R. Cohen, Editor. Guidebook on Molecular Modeling in Drug Design. Academic
Press, San Diego, 1996.

CO-PO MAPPING:

Course Code: BTE719 Course Title: MOLECULAR MODELLING &

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

DRUG DESIGN

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 2 2 2
CO 2 3 2 2 2 2
CO 3 3 3 3 2 3 1 1 1 1 3

Department of Biotechnology
Course Title of the course
Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE720 NANOTHERAPEUTICS PEL 3 0 0 3 3

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
CT+EA
Course CO1:To understand the role of the small molecules in the drug delivery system.
Outcomes
CO2: To learn the fundamentals and principles of nanotechnologies in drug release
system.

CO3: To understand methods of nanotechnology in point of care diagnosis.

CO4: To understand the basic mechanism of nanotherapeutics of tumours.

Topics UNIT -I NANOPHARMACEUTICALS

Covered
Nano-biotechnology for Drug Discovery -Gold Nanoparticles for Drug Discovery -
Use of Quantum Dots for Drug Discovery -Nanolasers for Drug Discovery -Cells
Targeting by Nanoparticles with Attached Small Molecules .
5

Dendrimers ,Nanobodies, Nanospheres-Nanotubes –Nano-cochleates.- Nano-

molecular Valves for Controlled Drug Release –Nano-motors for Drug Delivery.
6

UNIT - II ROLE OF NANOTECHNOLOGY IN BIOLOGICAL THERAPIES

Development of nano medicines – Nano Shells – Nano pores – Tectodendrimers –

Nanoparticle drug system. Biomedical nanoparticles –Liposome’s Different types of
drug loading – Drug release – Biodegradable polymers.
5

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Applications Nano biotechnologies for Single-Molecule Detection -Protease-

Activated Quantum Dot Probes.
3

Nanotechnology for Point-of-Care Diagnostics –Nano diagnostics for the Battle Field
– Nano diagnostics for Integrating Diagnostics with Therapeutics.
4

UNIT – III APPLICATION IN CANCER THERAPY & NANOMEDICINE

Introduction and Rationale for Nanotechnology in Cancer Therapy -- Diagnostic

approach by nano-sensing. 3

Passive Targeting of Solid Tumors: Pathophysiological Principles and

Physicochemical Aspects of Delivery Systems -Active Targeting Strategies in Cancer
with a Focus on\Potential Nanotechnology Applications.
5

Pharmacokinetics of Nano-carrier-Mediated Drug and Gene Delivery. 4

Text Books, References:

and/or
reference 1. Kewal K. Jain , The Handbook of Nano-medicine Humana Press, (2008).
material 2. Zhang, Nanomedicine: A Systems Engineering Approach” 1st Ed., Pan Stanford
Publishing, (2005).
3. Robert A. Freitas Jr., ―Nano-medicine Volume IIA: Biocompatibility, Landes
Bioscience Publishers, (2003).

CO-PO MAPPING:

Course Code: BTE720 Course Title: NANOTHERAPEUTICS

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 1 2 3 3 3 1 1 2 0 1 2 2
CO2 2 3 3 3 2 3 3 2 1 1 1 2
CO3 3 3 3 3 3 1 2 2 2 1 2 1
CO4 1 2 3 2 3 1 1 3 1 1 1 3

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE721 BIOMATERIALS PEL 3 0 0 3 3

BT C303 Biochemistry & Course Assessment methods (Continuous (CT) and end assessment
Enzyme Technology, CYC01 (EA))
Chemistry
CT+EA
Course  CO1: Classify the biomaterials and recognize their production and properties.
Outcomes  CO2: Explain the application areas of biomaterials
 CO3: To realize the important basic properties and requirements for biomaterials
 CO4: Recognize the importance of relationships between living tissues and
biomaterials
Topics  Definition of biomaterials – biologically derived materials or materials compatible
Covered with biology. (2)
 Common biomaterials: some proteins, many carbohydrates and some specialized
polymers. (4)
 Collagen (protein in bone and connective tissues): Structure production and its use.
(3)
 Fibroin (protein in silk): Production and its use. (2)
 Production of these proteins by conventional cloning methods. (3)
 Carbohydrates: Modified carbohydrates acting as lubricants for biomedical
applications; Polydextrose; Carbohydrates modified by enzymes; (8)
 Biopolymers: Synthesis from a simple biological monomer ( eghyaluronate
polymers); Dextrans (used in chromatography columns); Rubberllike materials
produced by bacteria and fungi (Polyhydroxybutyrate PHB),
Polycaprolactone(PCL); Production of a copolymer of PHB and
PHV(polyhydrovaleric acid), sold as Biopol by fermentation by
Alcaligeneseutrophus; Biodegradable polymers (8)
 Industrial biopolymers: Production of polyphenol resins by the enzyme soybean
peroxidase; Evaluation of the properties of biopolymers to make good biomaterials;
Tensile strength (both elasticity and breaking strength); Hydration, visco – elastic
properties; viscosity. (8)
 Biomaterials for Organ Replacement; Tissue Engineering; tissue replacements,
cardiovascular; biodegradable and bioactive materials, drug delivery systems. (4)
Text Books, Text Book:
and/or 1. Biomaterials: Principles and Applications by J.B. Park and J.D. Bronzino.
reference 2. Biomaterials: SUJATA V. BHATT, Second Edition, Narosa Publishing House,2005.
material 3. Biomaterials Science: An introduction to Materials in Medicine, Edited by Ratner,
Hoffman, Schoet and Lemons, Second Edition: Elsevier Academic Press, 2004.

Reference book:
1. Biomaterials Science and Biocompatability, Fredrick H. Silver and David L.
Christiansen, Piscataway, Springer, New Jersey.

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING:

Course Code: BTE721 Course Title: BIOMATERIALS

PO1 PO2 PO3 PO 4 PO 5 PO 6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 3 3 3 2 2 1 3 1 3 3 3

CO 2 3 3 3 2 2 1 3 1 3 3 3

CO 3 3 3 3 3 2 1 3 1 3 3 3

CO 4 3 3 3 2 3 1 3 1 1 3 3 3

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTE722 VACCINE PEL 3 0 0 3 3
TECHNOLOGY
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
BTC402/BT501 CT+EA
Immunology
Course  CO1: To understand the factors that influence vaccine design and development
Outcomes  CO2: To understand how research based discovery has driven vaccine
development
 CO3: To know about the different types of vaccines
 CO4: To learn about the quality control and regulation in the vaccine production
 CO5: To understand the importance of vaccination as a public health strategy
Topics History of vaccine development- Importance of vaccines (2)
Covered Immunological response to vaccines (2)
Vaccine design and development: Epitope identification; Vaccine efficacy, Adjuvants
(6)
Different types of vaccines: Inactivated toxins, Inactivated whole bacteria or viruses,
Live attenuated bacteria or viruses; Subunit vaccines, Polysaccharide vaccines,
Conjugated vaccines ; Recombinant DNA vaccines, Edible vaccines, Virus like
particles(8)
Next-generation vaccines: Human Immunome project; Human antibodies as vaccines
(4)
Production techniques used for vaccines (4)
Storage and preservation of vaccines (4)
Delivery methods: microspheres, nanoparticles; ISCOMS and immunomodulators (6)
Regulatory issues in vaccine production: OIE guidelines for production and seed lot
management; Manufacturing recommendation; Final product release tests (5)

90
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Vaccine safety-the debate (1)

Text Books, Text Books:

and/or 1. New Vaccine Technologies: Ronald W. Ellis (Landes Bioscience), 2001.
reference 2. Vaccines: Stanley A. Plotkin, Walter A. Orenstein, Paul A. Offit(Elsevier), 6th
material Edition
Reference Books:
1. Medical Microbiology : Samuel Baron , 4th Edition (University of Texas)
2. Advances in Vaccine Technology and Delivery: Cheryl Barton, Espicom Business
Intelligence.
3. “Vaccine manual: The production and quality control of veterinary vaccines for
use in developing countries”: Noel Mowat ,Daya books.

Course Code: BTE722 Course Title: VACCINE TECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 2 1 1
CO2 2 3 2 1
CO3 2 2 1 2
CO4 2 2 2 1 1 2
CO5 1 2 2

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTE723 STEM CELL PEL 3 0 0 3 3
BIOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
Cell Biology, CT+EA
Biochemistry, Genetics,
Molecular Biology
Course  CO1: To understand the basic mechanisms of how cells differentiate into specific
Outcomes tissues in response to a variety of biologic signaling molecules and the use of such

91
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

factors for tissue production in-vitro.

 CO2: To acquire knowledge on the molecular basis of cellular and functional
changes of different organs that occur in disease and treatments that cause tissue
remodeling to correct these changes
 CO3: To gather insights on how studies of the developmental, cellular and
molecular biology of regeneration have led to the discovery of new drugs/therapy
for regenerative therapy.
 CO4: To understand the recent advances on application the regenerative therapy
from well characterzied case studies.

Topics 1. An Introduction to Stem Cells (2)

Covered 2. Adult Stem Cells (1)
3. Embryonic Stem Cells (1)
4. Induced Pluripotent Stem Cells (1)
5. Hematopoietic Stem Cells (1)
6. Messenchymal stem cells , cord blood cells, Lessons from Medipost company
products like Neurostem, Cardiostem, Cartistem, Pneumostem (4)
7. Molecular and Cellular Bases of Organ Development (6)
8. Cloning of Somatic Cells by Nuclear Transfer, iPSC based cloning, Production
of chimera animals (4)
9. Molecular Bases of degenerative disease (1)
10. Therapeutic Uses of Stem Cells with examples (2)
11. In vivo Regeneration of Tissues by Cell Transplantation (2)
12. IPS Cells as Experimental Models of Neurodegenrative Disorders: use of them
as disease modelling platform, novel drug testing and tissue renerarative
therapy and implantation studies(2)
13. Studies of Patients Treated with Stem Cells, The modalities of treatment,
Preperation of cells/tissues/scaffolds and Trnasplantation procedure (3)
14. Tissue Regeneration Driven by Growth Hormones (2)
15. Organ of dish, Orgnoid culture, Tissue Bioprinting to develop transplantation
quality organs, Bioartificial Organs (8)
16. Biobanking of stem cells and the ethical considerations in regenerative
medicine. (2)
Text Books, Text Books:
and/or 7. Stem Cells, Tissue Engineering And Regenerative Medicine By: David Warburton
reference 1st Edition.
material 8. Principles of Regenerative Medicine by Anthony Atala Robert Lanza Tony Mikos
Robert Nerem , 3rd Edition.
9. Translational Regenerative Medicine by Anthony Atala and Julie G. Allickson

Reference Books:
1. The Developping Human by Keith L. Moore/T.V.N. Persaud/ Mark G. Tenth
edition.
2. Encyclopedia of Tissue Engineering and Regenerative Medicine by Rui Reis,
IstEdtion.

92
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO-PO MAPPING:

Course Code: BTE723 Course Title: STEM CELL BIOLOGY

PO1 PO2 PO 3 PO 4 PO 5 PO 6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 2 1 1 3 1 1 2 - - 2 - 1
CO 2 2 1 2 3 2 2 2 - - 2 - -
CO 3 2 2 3 2 3 3 3 - 3 2 - 2
CO 4 3 2 3 3 2 2 3 - 3 2 - 2

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR)
/ Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTE724 APPLICATIONS PEL 3 0 0 3 3

OF
MOLECULAR
CLONING

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))

BTC401 (Molecular Biology CT+EA

& rDNA Technology)

Course CO1: To understand the fundamentals of molecular cloning.

Outcomes
CO2: To learn the basic methods of molecular cloning.

CO3:To gain knowledge about the potential application aspects of molecular cloning.

CO4: To build-up a bridging concept for extension of theoretical knowledge to

practical applications of molecular cloning.

Topics Module 1: Basic principles of molecular cloning

Covered
- Why gene cloning and DNA analysis are important (2)
- Vectors for gene cloning (2)
- Purification of DNA from living cells (2)
- Manipulation of purified DNA (3)
- Introduction of DNA into living cells (3)
- Cloning vectors for prokaryotes (3)
- Cloning vectors for eukaryotes (3)
- How to obtain a clone of a specific gene (2)
93
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

- Other molecular techniques (2)

Module 2: Applications of molecular cloning in research

- Sequencing genes & genomes (3)

- Studying gene expression & function (3)
- Studying genomes (4)
Module 3: Applications of molecular cloning in biotechnology

- Production of protein from cloned genes (2)

- Gene cloning & DNA analysis in medicine (3)
- Gene cloning & DNA analysis in agriculture (3)
- Gene cloning & DNA analysis in forensic science &environment (2)
Text Books, Text Books:
and/or 1. T. A. Brown, Gene Cloning and DNA Analysis: An Introduction, Seventh
reference Edition, Wiley Blackwell.
2. Sandy B. Primrose, Richard Twyman & Bob Old, Principles of gene
material
manipulation primrose: An introduction to genetic engineering, Sixth Edition,
Blackwell Science

CO-PO MAPPING:

Course Code: BTE724 Course Title: APPLICATIONS OF

MOLECULAR CLONING

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 0 0 2 0 0 2 0 2 0 0 1

CO2 2 0 0 2 0 0 2 0 2 0 0 1

CO3 2 2 3 0 3 3 2 2 2 0 0 2

CO4 3 3 2 0 2 2 3 2 2 0 0 3

Course Title of the Program Total Number of contact hours Credit

Code course Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTO740 GENETIC PEL 3 0 0 3 3
ENGINEERING
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
NIL CT+EA
Course CO1:Students will acquire basic understanding of molecules of life and their basic
Outcomes chemistry.
CO2:Students will acquire knowledge of how genetic material stores programs of life
and how that information is retrieved.
CO3: Students will acquire knowledge of basic tools of genetic engineering and their
94
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

applications.
CO4:Students will be able to apply the acquired knowledgein understanding and
solving biotechnology issues surrounding us.
Topics 1. Structures of macromolecules such as Carbohydrates, Proteins, Enzymes, Lipids
Covered and Nucleic Acids. [10]
2. Basics of cell biology, prokaryotes vs. eukaryotes, sub-cellular structures, their
organization and functions. [10]
3. Central Dogma of molecular biology, DNA Replication, Transcription, Reverse
Transcription, Translation. [10]
4. Basic tools of nucleic acid manipulation. Methods of genetic engineering; Genetic
engineering of microbes, plants and animals.[12]
Text Books, Text:
and/or 1. Essential Cell Biology, 4th Edition, Alberts et. al.
reference 2. Biotechnology.2nd Edition, 2015. David Clark and Nanette Pazdernik.Academic
material Cell.
3. Cecie Starr, Christine A. Evers, Lisa Starr. Biology: Today and tomorrow with
physiology.
Reference:
1. Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts and
Peter Walter, Molecular Biology of the Cell, Garland Science.
2. Molecular Biology of the Gene by James D. Watson, Tania A. Baker, Stephen P.
Bell, Alexander Gann, Michael Levine, Richard Losick.

CO-PO MAPPING:
Course Code: BTO 740 Course Title: GENETIC ENGINEERING

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1

CO2 2 1

CO3 2 2 2 1

CO4 1 1 2 1

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
95
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Code Core (PCR) Lecture Tutorial Practical Total

/ Electives (L) (T) (P) Hours
(PEL)
BTS751 BIOSEPARATION PCR 0 0 3 3 1.5
AND
BIOCHEMICAL
ANALYSIS
LABORATORY
Pre-requisites Course Assessment methods (Continuous assessment (CA) and end-
term examination (ET))
Bioseparation& Biochemical CA+ET
Analysis (BTC 503)
Course  CO1: To determine the specific cake resistance & filter medium resistance
Outcomes by constant pressure filtration/pressure-time variation in constant rate
filtration
 CO2: To prepare a cell-free extract by sonication/homogenization and
identify a specific protein therein by Western Analysis
 CO3: To learn the technique of salt precipitation of a protein and
subsequent dialysis for removal of the salt and to get an idea of other
equipment for concentrating a protein
 CO4:To construct a binodial diagram and study the extraction of a protein
in an aqueous two-phase system
 CO5:To separate out a protein from a mixture by gel filtration/ion exchange
chromatography and to concentrate a protein by ultrafiltration
 CO6: To extract and estimate biomolecules such as lipids, DNA, & RNA
Topics 1. Filtration (constant pressure filtration)
Covered 2. Preparation of cell-free extracts from cultured cells
3. Salt precipitation of protein and Dialysis
4. Extraction and estimation of total lipid content
5. Separation/concentration of proteins by Ultrafiltration.
6. Aqueous two phase extraction (binodial diagram)
7. Separation of proteins by gel permeation/ion-exchange chromatography
8. Identification of a specific protein present in the cell-free extract by Western
Analysis
9. Determination of DNA and RNA concentration by UV absorption
10. Demonstration of lyophilization& Rotary vacuum evaporation

Text Books, Textbooks :

and/or 3. Practical Biochemistry Principles and techniques (5 thed)/ Principles and
reference Techniques of Biochemistry and Molecular Biology (7 thed): Editor Wilson
material and Walker, Cambridge University Press
4. Geankoplis, Transport Processes & Unit operations, PHI.
Reference books:
4. D. Holme & H. Peck, Analytical Biochemistry, 3 rded, Longman, 1998
5. Shuler & Kargi, Bio-process Engg. PHI
6. Bailey &Olis, Biochemical Engg. Fundamentals, McGraw-Hill

CO-PO MAPPING:

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course Code: BTS751 Course Title: BIOSEPARATION AND

BIOCHEMICAL ANALYSIS LABORATORY

PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12

CO 1 2 - - - - - - - 1 2 - -
CO 2 2 1 - 2 1 1 1 1 2 2 - 1
CO 3 1 - 1 - 1 - 1 - 1 2 1 2
CO 4 1 - 1 - - - - - 1 2 1 -
CO 5 1 - 2 1 1 - 1 - 2 2 - 1
CO 6 1 - - 1 1 1 - 1 1 2 - 1

Department of Biotechnology
Course Title of the Program Total Number of contact hours Credit
Code course Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTS752 CELL & PCR 0 0 3 3 1.5
TISSUE
CULTURE
LABORATORY
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
BTC01 Life Science CT+EA
BTC301 Cell Biology and
Genetics
BTC 502 Cell and Tissue
Culture
Course CO1: Students will be acquainted with basic plant tissue culture techniques.
Outcomes CO2: Students will be acquainted in basic animal cell culture techniques.
CO3: Students will attain knowledge of application of cell and tissue culture techniques
in academic and industrial laboratories.
CO4: Students will have knowledge of biosafety and ethical issues related to cell and
tissue culture.
Topics Plant Tissue Culture
Covered 1. Preparation and sterilization of plant tissue culture media.
2. Preparation of explants.
3. Callus induction in rice.
4. Regeneration of rice callus tissue.
5. Rooting of regnerants in rice.
Animal Cell Culture
6. Sterilization Techniques, Preparation of Media & Preparation of Sera
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

7. Primary Cell Culture

8. Preparation of established Cell lines
9. Cell Counting and Viability
10. Staining of Animal Cells & Preservation of Cells
Text Books, 1. Laboratory manual.
and/or
reference
material

CO-PO MAPPING:

Course Code: BTS752 Course Title: CELL & TISSUE CULTURE LABORATORY

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1 1 1 1

CO2 2 1 1 1 1

CO3 2 1 1 1 1

CO4 2 1 1 1

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTS BIOCHEMICAL 0 0 3 3 1.5
753 REACTION
ENGINEERING
LABORATORY
Pre-requisites Course Assessment methods (Continuous (CT) and end assessment
(EA))
CT+EA
Course 1. To learn the experimental protocol of microbial growth and inhibition kinetics in a
Outcomes batch process
2. To study substrate degradation, cell growth and product formation with
immobilized cells in plug flow bioreactors.
3. To learn about functions of a fermenter
4. To study non-ideality in a plug flow reactor
Topics 1. Microbial cell growth kinetics
Covered
2. Microbial cell inhibition kinetics

3. Substrate degradation, cell growth and product formation study using

immobilized cells in a continuous packed bed reactor.

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

4. Substrate degradation, cell growth and product formation study using

immobilized cells in a continuous fluidized bed reactor.

5. Function of bioreactor- a) calibration of DO electrode. b) Calibration of pH

electrode.

6. RTD studies in a packed bed reactor

Text NA
Books,
and/or
reference
material

CO-PO MAPPING:

Course Code: BTS753 Course Title: BIOCHEMICAL REACTION ENGINEERING

LABORATORY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1 1 2 1 2 3 2 2

CO2 2 1 1 2 1 2 3 2 2

CO3 2 1 1 2 1 2 3 2 2

CO4 2 1 1 2 1 2 3 2 2

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTS VOCATIONAL PCR 0 0 3 3 3
754 TRAINING /
SUMMER
INTERNSHIP
AND SEMINAR

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
NA CT+EA
Course  CO1: To learn literature mining and acquire knowledge of presenting data in a
Outcomes
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

proper format
 CO2: To enhance the communication skills of students
 CO3: Enable the students to face various kinds of audiences and develop self-
confidence
 CO4: To learn application of ethical principles in various fields of research
Topics Each student is allotted a slot where he/she presents a scientific topic (related to the
Covered summer training they did in the previous semester)
Text Text Books:
Books, N.A.
and/or
reference
material

CO-PO MAPPING :
Course Code: BTS754 Course Title: VOCATIONAL TRAINING /
SUMMER INTERNSHIP AND SEMINAR

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 3 3 2 3 2 2 2 2 1 3 3 3

CO 2 1 2 1 2 2 1 1 1 3 3 3 3

CO 3 1 2 1 2 1 1 1 1 3 3 3 3

CO 4 3 2 3 3 2 3 2 3 3 2 2 3

Department of Biotechnology

Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) /
Electives (PCR) Lecture Tuto Practical Total
(L) rial (P) Hours
(T)

BTS755 PROJECT-I PCR 0 0 3 3 1

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment (EA))

All the Program Core CT and EA

subjects

Course CO1: To design, analyze and solve biological, clinical and biotechnology related research

100
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Outcome problem problems through participating in scientific project works.

s
CO2: Familiarization with recent researches in the field of biotechnology.

CO3: To develop skills to perform experiments, get familiar with different cutting edge
technologies used to answer research questions and have hands on training on the related
area.

CO4: To learn to interpret data, draw conclusion and develop trouble shooting skills.

CO5: To learn to present data, and defend a hypothesis forming the basis of a scientific
study.

Topics 11. Each student has to choose a Principle Investigator depending on his/her research
interest and inclination and has to get involved in any ongoing research project.
12. Students are required to familiarize themselves with the literature review and
scientific techniques and skills.

Text Reference
Books,
and/or  Related research papers.
reference
material

CO-PO MAPPING:

Course Code: BTS755 Course Title: PROJECT-I

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 3 3 3 2 2 2 3 3 3

CO2 3 2 2 3 2 2 1 1 1 2 3 3

CO3 3 3 3 2 2 2 1 3 3 1 3 3

CO4 3 3 3 2 3 3 2 3 2 2 3 3

101
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO5 3 3 3 3 3 3 2 3 3 3 3 3

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE810 PLANT PCR 3 0 0 3 3
DEVELOPMENTAL
BIOLOGY
Pre-requisites Course Assessment methods (Continuous (CT) and end
Plant Molecular Biology and assessment (EA)
Genetics
NIL CT+EA
Course CO1: Students will Learn about the roles of light and various phytohormones in plant
Outcomes growth and development.
CO2: Students will acquire knowledge about soot and root apical meristems.
CO3: Students will Learn about the effect of different environmental factors on plant
growth and development.
CO4: Students will be able to apply the acquired knowledge in understanding and
solving biotechnology issues in a societal context.
Topics Embryogenesis and Organogenesis (4)
Covered Soot and root apical meristem (2)
Growth of seedlings (5)
Environmental Factor (2)
Totipotency (4)
Phototropism and gravitropism (3)
Plant morphology (2)
Photomorphogenesis (6)
Phytohormones (4)
Text Books, 1.Lewin B: Genes (VI and above Edition).
and/or 2. Albert, B: Molecular Biology of the Cell (any Edition).
reference 3. Research articles will be given by the teacher.
material

CO-PO MAPPING:
Course Code: BTE810 Course Title: PLANT DEVELOPMENTAL
BIOLOGY

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

102
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO1 2 2 1

CO2 1 1 2

CO3 2 2 1 2 2 1

CO4 1

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) Lecture Tutorial Practical Total
/ Electives (L) (T) (P) Hours
(PEL)
BTE811 BIOPROCESS PEL 3 0 0 3 3
PLANT &
EQUIPMENT
DESIGN

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
CT+EA
Course
Outcomes
Topics Plant Location and Site Selection: (5)
Covered
 Site and Plant Layout
 Utilites
 Storage Methods and Material Handling
 Plant operation and Control systems
 Environmental considerations
Conventional and unconventional bioreactors and their Design:
(12)
 Batch, Continuous stirred tank bioreactors (CSTBR)
 Plug flow bioreactors
 Enzyme and immobilized bioreactors
 Fluidized bed bioreactors,
 Bubble column bioreactors and Air- lift bioreactors
 Hollow- fiber bioreactors
 Membrane bioreactors
 Bioreactors for plant and animal cell culture systems
 Ideal and non ideal reactors
Sterilization of Bioreactors:
(4)
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

 Design of Batch and Continuous Media Sterilizers

 Design of Air Sterilizers.

Instrumentation and Control of Bioprocesses:

(4)

 Physical and chemical environmental sensors

 Computer control of bioreactors
Modelling and Simulation of Bioprocesses:
(2)
 Study of structured and unstructured models for analysis of various processes

Design of Bioreactor systems:

(6)

 Design of Filtration and Centrifugation equipments

 Design of Driers.
 Refrigeration systems
 Steam Generation systems
 Pumps
Cost Analysis in Bioprocess Engineering:
(2)
 Estimation of capital investment and operating cost

Text Books, Text Books:

and/or
reference 1.Bioprocess Engineering Principles, by Pauline M.Doran Academic Press
material
2. Bioprocess Engineering ,Kinetics, Biosystems, Sustainability and Reactor

Design by Shijie Liu Elsevier

3. Coulson & Richardson’s Chemical Engineering Vol.6 Butterworth-Heinemann

Reference Books:

1. Plant design and Economics for chemical engineers by Peter M. S. Timmerhaus,

K. D. McGraw Hill.
2. Coulson & Richardson’s Chemical Engineering Vol.3 Butterworth-Heinemann

CO-PO MAPPING:

Course Code: BTE811 Course Title: BIOPROCESS PLANT &

EQUIPMENT DESIGN

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 3 3 3 2 2 3 3 3 2 3 3 2
CO 2 3 3 3 3 3 2 3 2 3 3 3 3
CO 3 3 3 3 2 2 2 2 2 3 2 2 2

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTE812 MEDICAL & PEL 3 0 0 3 3
PHARMACEUTICAL
BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
CT+EA
Course
Outcomes
Topics Introduction - Biopharmaceuticals and their development, historical aspects, general
Covered steps in development of a drug, sources and strategies (including random, non-
random, and rational) of discovering lead compounds
2
Drug designing

Macromolecules as Targets of drugs: (lipids, carbohydrates, proteins, nucleic acids)

2

Drug targets: carrier proteins, structural proteins, enzymes, receptors (including

mechanisms – ion channels and membrane-bound enzymes)
4

Concepts and design criteria of agonists, antagonists, partial agonists, and inverse
agonists. 3

Rational drug designing, Structure –activity relationships and identification of

pharmacophore and auxophore in a lead compound; drug design on the basis of drug-
target interactions.
5

Disease diagnosis
PCR, LCR immunological assay, Detection of genetic, Neurogenetic disorders
involving Metabolic and Movement disorders. Treatment-products from recombinant
and non-recombinant organisms, Interferons, Antisense therapy, cell penetrating
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

peptides. Gene therapy, Types of gene therapy, somatic virus germline gene therapy,
mechanism of gene therapy, Immunotherapy. Detection of mutations in neoplastic
diseases MCC, SSCP, DGGE, PTTC. Use of enzymes in clinical diagnosis. Use of
biosensors for rapid clinicalanalysis. Diagnostic kit development for microanalysis,
Diagnosis of disease by proteomics.
25
Production of pharmaceuticals
Production of pharmaceuticals by genetically engineered cells (hormones, interferons).
Microbial transformation for production of important pharmaceuticals (steroids and
semi-synthetic antibiotics). Techniques for development of new generation antibiotics.
15

Drug delivery

Text Books, Textbooks:

and/or
reference 1. An Introduction to Medicinal Chemistry; Graham L.Patrick, Oxford
material
Reference Books:

1. The Organic Chemistry of Drug Design and Drug Action; Richard B.

Silverman, Elsevier

CO-PO MAPPING:

Course Code: BTE812 Course Title: MEDICAL &

PHARMACEUTICAL BIOTECHNOLOGY

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 1 1 - 1 - - 1 - - - -

CO2 2 1 1 - 1 - 1 - - - - 1

CO3 2 1 1 - 1 - 1 - - - - 1

CO4 2 1 1 - 1 - - 1 - - 1 1

Department of Biotechnology
Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) / Lecture Tutorial Practical Total
Electives (L) (T) (P) Hours
(PEL)
BTE813 GM CROPS PEL 3 0 0 3 3

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))
BTC402 (Cell & Tissue CT+EA
Culture of Animals &
Plants)
Course CO1: Development of knowledge of natural resistance / tolerance to various biotic and
Outcomes abiotic stress to plants.
CO2: Development of ability to design strategy to genetically modify crop plants for
quality improvement.
CO3: Learning about the strategies toward generating environment friendly GM crops.
Topics Introduction [2]
Covered Methods of genetic transformation [4]
Genetic engineering of resistance to biotic stress [6]
Genetic engineering of tolerance to abiotic stress [6]
Genetic engineering for removal of environmental pollutants [4]
Genetic engineering for quality nutrition and health [4]
Genetic engineering for molecular farming [4]
Biosafety concerns [4]
Removal of selectable markers from GM crops [4]
Modern tools of genetic manipulation of plants [4]
Text Books, Text Books:
and/or 1. H.S.Chawla, Introduction to Plant Biotechnology, Oxford & IBH Publishing co.
reference Pvt..Ltd
material 2. Slater.A.,NigelW.S,Flower.R.Mark , Plant Biotechnology: The Genetic
Manipulation of Plants, 2003, Oxford Univesity Press.
Reference Book:
3. Buchaman, Gursam, Jones, Biochemistry and Molecular Biology of Plants, 1ed,
2000, L.K.International.
4. Bhojwani and Razdan –Plant Tissue Culture: Theory and Practice 1996 Elsevier

CO-PO MAPPING:

Course Code: BTE813 Course Title: GM CROPS

COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 1 1 2 1 1 1 1 1

CO2 1 2 2 1 3 2 2 3 2 1 1 2

CO3 1 2 3 2 3 2 2 1 2 1 1 2

Department of Biotechnology

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course Title of the Program Total Number of contact hours Credit

Code course Core (PCR) /
Electives Lecture Tutorial Practical Total
(PEL) (L) (T) (P) Hours

BTE814 BIOETHICS PEL 3 0 0 3 3

AND IPR

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment

(EA))

CT+EA

Course CO1: To understand the nature of hazards related to biotechnology and the importance
Outcomes of biosafety in research.

CO2: To learn and debate on different ethical issues of applications of Biotechnology

research including recombinant DNA technology and Human trials.

CO3:To realize the importance and basics of intellectual property Rights and laws
implemented in this regard.

CO4: To learn the basic way to file claim of a patent.

CO5: To understand the idea about Entrepreneurship and its economic implication in
the area of biotechnology research

Topics Biotechnology and Society: Introduction to science, technology and society,

Covered biotechnology and social responsibility, public acceptance issues in biotechnology,
issues of access, ownership, monopoly, traditional knowledge, biodiversity, benefit
sharing, environmental sustainability, public vs. private funding, biotechnology in
international relations, globalization and development divide. (8)

Bioethics: Legality, morality and ethics, the principles of bioethics: autonomy, human
rights, beneficence, privacy, justice, equity etc. (6)

Biotechnology and Bioethics: The expanding scope of ethics from biomedical practice
to biotechnology, ethical conflicts in biotechnology - interference with nature, fear of
unknown, unequal distribution of risks and benefits of biotechnology, bioethics vs.
business ethics. (7)

Ethical dimensions of IPR, technology transfer and other global biotech issues.
Jurisprudential definition and concept of property rights, duties and their correlations,
history and evaluation of IPR – like patent design and copyright. Distinction among the
various forms of IPR, requirements of a patent able invention like novelty, inventive
step and prior art and state of art. (8)

Regulations on ethical principles in biomedical/ biotechnological practice: The

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Nuremberg code, declaration of Helsinki; the Belmont report, co operational guidelines

– WHO, guidelines of DBT (India), Guidelines of an informed consent

Rights/ protection, infringement or violation, remedies against infringement, civil and

criminal, Indian patent act 1970 and TRIPS major changes in Indian patent system,
post-TRIPS effects. (7)

Contents of patent specification and procedure for obtaining

a) patents

b) Geographical indication,

c) WTO

Detailed information on patenting biological products, Biodiversity (6)

Text Books, Textbook:

and/or 1. F. H. Erbisch and K. M. Maredis, Intellectual Property Rights in Agricultural
reference Biotechnology, Bios Publishers
material
Text / Reference Books:
1. Thomas, J.A., Fuch, R.L. (2002). Biotechnology and Safety Assessment (3rd Ed).
Academic Press.
2. Fleming, D.A., Hunt, D.L., (2000). Biological safety Principles and practices (3rd
Ed). ASM Press, Washington.
3. Biotechnology - A comprehensive treatise (Vol. 12). Legal economic and ethical
dimensions VCH.
4. Encyclopaedia of Bioethics

CO-PO MAPPING:

Course Code: BTE814 Course Title: BIOETHICS AND IPR

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 1 2 2 2

CO2 1 2 1 3

CO3 1 1 2 2

109
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

CO4 1 1 2 2

CO5 1 2 1 1 2 2

Department of Biotechnology

Course Title of the course Program Total Number of contact hours Credit
Code Core (PCR) Lectur Tutoria Practical Total
/ Electives e (L) l (T) (P) Hour
(PEL) s
BTE815 ENVIRONMENTAL PEL 3 0 0 3 3
MICROBIOME
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
Microbiology and Bioprocess CT+EA
Technology (BTC302); Molecular
Biology and recombinant DNA
Technology (BTC401) ;
Bioinformatics (BTC601)
Course CO1: Develop understanding of Microbial Diversity and Ecology. Understand the
Outcomes Physicochemical and biological factors that define the microbiome in different
environments as well as the significance of microbial interaction with environment
CO2: Learn about the important tools and techniques used to study microbial ecology
or microbiome structure. Learn to apply “Omics” approaches to assess the microbial
community structure and function.
CO3: Understand the System biology approach to assess the interaction and function of
microbiome members in global scale.

CO4: Learn to exploit microbial community members for Resource recovery,

Environmental clean-up, CH4 production and consumption, CO2 sequestration, etc.

Topics Introduction- Significance, developments and challenges of environmental

Covered microbiome study. (4)
Microbial Diversity and ecology- Environments and microenvironments, ecosystem
services, biogeochemistry and nutrient cycles, carbon-nitrogen-sulfur-and other
nutrient cycles. (7)
Survey of microbiome in different habitats- Microbiomes of Terrestial, Marine,
Freshwater, Deep sea, Hydrothermal vents, Subsurfaces, Permafrost region etc. Earth
microbiome and Human microbiome Project. (7)
Microbiome of the built environment- Microbial interactions with environment,
microbial influenced corrosion, microbial enhanced oil recovery, mineral recovery,
bioremediation of heavy metals and organic pollutants, methane production and
consumption (7)
Microbiome characterization- Metagenomics, metaproteomics and
metatranscriptomics, culture dependent and culture independent techniques,
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

conventional and molecular analyses, assessment of microbial metabolic diversity and

activities. (8)
System Biology and Microbial interaction- Approach of system biology in
bioremediation, bioremediation with genomics, interaction between community
members within microbiome, commensalism, syntrophism, interspecies hydrogen
transfer etc. Strategies of bioremediation, Microbial performance assessment. (9)

Text Books, Text Book

and/or Brock Biology of Microorganisms- Madigan, Martinko, Bender, Buckley and Stahl-
reference Pearson publisher.
material Bioremediation and Natural Attenuation: Process Fundamentals and Mathematical
models- P J J Alvarez and W A Illman- Wiley Interscience.
Reference Books
Environmental Microbiology: from genomes to biogeochemistry- Eugene L.Madsen-
Blackwell Publishing.
Environmental Microbiology for Engineers- V.Ivanov- CRC Press.
Environmental Microbiology- Maier, Pepper and Gerba- Elsevier (Academic Press).

CO-PO MAPPING:
Course Code: BTE815 Course Title: ENVIRONMENTAL
MICROBIOME

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO 1 2 2 2 2 2 2 2 2 2 2 3 3

CO 2 3 3 3 3 3 2 2 2 2 3 3 3

CO 3 2 3 3 2 3 3 3 3 3 3 3 3

CO4 3 3 3 3 3 3 3 3 3 3 3 3

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTO840 INDUSTRIAL PEL 3 0 0 3 3
BIOTECHNOLOGY

Pre-requisites Course Assessment methods (Continuous (CT) and end

assessment (EA))
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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Life science CT+EA

Course CO1- To understand the methods of cell 's bio processing under various conditions,
Outcomes strain improvement methods for better results
CO-2 Demonstrate the experimental techniques associated with aseptic processes,
media preparation and related upstream processes
CO-3 .Design and develop medium for cell cultivation for fermentation process
Apply the knowledge of sterilization techniques
CO-4 Understand needs of various parts of fermenter and their operation and
Design bioreactor based on thumb rules for fermentation operation
CO-5 Apply the knowledge of Purification Separation and kinetics theory of
Enzyme production for industrial fermentation

Topics UNIT 1 CELL CULTIVATION ,GROWTH KINETICS -- 10 Hrs

Covered Media development for Cell growth and culture for microbes , plant, animal -
derived cells and its application. Microbial growth kinetics, logistic growth model,
growth of filamentous organism Strain improvement of industrial micro organism.
Measurement of cell mass. Cell immobilization. Numericals..
UNIT 2-MEDIA PREPARATIONand STERILIZATION 10 Hrs
Sterilization: basic concepts in sterilization insitu and ex-situ sterilization,
Sterilization of medium, air, filters, fermenter. Types of media, Strain preservation ,
inoculum preparation, Development of inocula for industrial fermentation/ seed
fermenter
UNIT 3- BIOREACTOR DESIGN AND ITS OPERATION- 12 Hrs
Purpose and importance of bioreactor, Parts of fermenter and types ;Oxygen
requirement, Oxygen transfer in fermenter, , KLa measurement, Measurement of
dissolved oxygen concentrations, Estimating Oxygen Solubility'Operational modes
of bioreactor: batch, semi-batch/fedbatch, continuous. Major components of
bioreactor and its purpose, classification of Bioreactor – SLF, SSF, animal and plant
cell culture. Classification of bioreactors for environmental control and management.
Fixed bed bioreactor, airlift reactor, hollow fibre reactor, seed reactor.
UNIT 4 INDUSTRIAL ENZYMES ,PURIFICATION and A PPLICATIONS -
10Hour
Enzyme engineered for new reactions-novel catalyst for organic synthesis. Case
studies: thermozymes cold adopted enzymes. Ribozymes, therapeutic enzymes of
industrial importance (amylase, glucose isomerase, cellulose, lipase, protease,
xylanase, invertase, peroxidases).
Separation of insolubles: filtration, centrifugation. Extraction and purification of
solubles: Ultra filtration, high performance tangential flow filtration, Recovery and
purification of intracellular products: cell disruption, chromatographic techniques.
Analytical assays of purity level of enzymes.

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Text Books, TEXT BOOKS: 1. Pauline M. Doran, “Bioprocess Engineering Principles”,

and/or Academic Press, 2 nd Ed., 2012. 2. El-Mansi (Ed.), “Fermentation Microbiology and
reference Biotechnology”, CRC Press, 3rd Ed., 2011. REFERENCE BOOKS: 1. Ashok Pandey
material
et al., “Enzyme Technology”, Springer Publisher, 2006. 2. Nielsen et al., “Bioreaction
Engineering Principles”, Plenum Publishers, 2nd Ed., 2002. 3. Mohammed A. Desai
(Ed.), “Downstream Processing of Proteins: Methods and Protocols”, Humana Press,
2000. 4. Satinder Ahuja, “Handbook of Bioseparations”, Vol 2, Academic Press, 1st
Ed., 2000.

CO-PO MAPPING

Course Code: BTO840 Course Title: INDUSTRIAL

BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 2 3 2 1 1 - -

CO2 2 3 1 3 2 2 - - -

CO3 1 1 2 2 2 - -

CO4 1 2 3 3 - 1 1

CO5 1 2 3 3 1 2 1

Department of Biotechnology
Course Title of the course Program Total Number of contact hours Credit
Code Core Lecture Tutorial Practical Total
(PCR) / (L) (T) (P) Hours
Electives
(PEL)
BTO850 MEDICAL PEL 3 0 0 3 3
BIOTECHNOLOGY
Pre-requisites Course Assessment methods (Continuous (CT) and end
assessment (EA))
CT+EA
Course  CO1: To provide an understanding about Inborn errors of metabolism and
Outcomes genetic disorders and their consequence.
 CO2: Able to analyze the key features therapeutics and drugs in current

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

scenario.
 CO3: Able to apply the knowledge for commercial production of
pharmaceuticals and place it in market for marketing approvals.
 CO4: Able to understand the ethical issues and the different competent
regulatory authorities globally associated with clinical Biotechnology.

Topics Microbial pathogenesis: Definitions - Infection, Invasion, Pathogen,Pathogenicity,

Covered Virulence, Carriers and their types, Opportunistic infections, NosocomialInfections,
epidemics.
Diagnosis of Infectious diseases–Biology of Nitric oxide implications in diagnosis
and therapeutics, Ethical problems around prenatal diagnosis, in vitro fertilization,
cloning, gene therapy.

DrugDesign and Drug delivery system : Synthesis of compounds in accordance

with the molecular structure and biological activity concept. Various principles/ mode
of drug action/ screening of drugs/ drug analysis using various techniques . New
generation viral vectors for Gene Therapy and advancement in Drug Delivery system,
antibody mediated drug delivery of vaccines, Antibiotics

Molecular Medicine: Antibodies and vaccines-Therapeutic production of antibodies

different kind of vaccines and applications of recombinant vaccines.Ribozymes for
therapeutic use in viral infection .

Cell and tissue therapy – Gene therapy, tissue engineering, stem cell and cloning.
In vivo targeted gene delivery
Clinical Toxicology,Clinical Research Governance and Ethics:

Basic concept in toxicology. Types and mechanism of toxin action- Epoxidation&

drug toxicity, Overview on regulatory affairs for pharmaceuticals, neutraceuticals and
medical devices. . International quality standard and related guidelines (ICH-E6).
Risk assessment and trial monitoring. Legal and ethical issues on biotechnology,
medical research and related clinical practice.

Text Books, Textbooks

and/or
reference 1. Recombinant DNA: Genes and Genomes - A Short Course, Third Edition
material (Watson, Recombinant DNA) by James D. Watson; Cold Spring Harbor
Laboratory Press

2. Biopharmaceuticals- Biochemistry and Biotechnology: Gary Walsh; John

Wiley & Sons

3. S. P. Vyas, V. Dixit, Pharmaceutical Biotechnology, CBS Publishers

4. Cedric A and Mim S. et al.: Medical Microbiology, Mosby USA

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Reference Books

1. Pharmaceutical Biotechnology ; Sambhamurthy&Kar , NewAge Publishers

2. Epenetos A.A.(ed), Monoclonal antibodies: applications in clinical oncology,

Chapman and Hall Medical, London

3. V.Venkatesharalu -Biopharmaceutics and Pharmacokinetics-Pharma Books

Syndicate
4. Diagnosis: A Symptom-Based Approach in Internal Medicine;
C.S.Madgaonkar, Publisher: JPB

CO-PO MAPPING:

Course Code: BTO850 Course Title: MEDICAL BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 2 1 1 2 2 1 - - - - - 2
CO 2 2 1 1 - 1 1 - 1 - 1 - 2
CO 3 2 1 1 1 1 1 - 1 - 1 1 2
CO4 2 1 1 1 1 2 2 2 1 1 2 2

Department of Biotechnology

Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) /
Electives (PEL) Lecture Tuto Practical Total
(L) rial (P) Hours
(T)

BTS855 Project-II PCR 0 0 15 15 5

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment (EA))

All the Program Core CT and EA

subjects

Course CO1: To design, analyze and solve biological, clinical and biotechnology related
Outcomes research problem problems through participating in scientific project works.

CO2: Familiarization with recent researches in the field of biotechnology.

CO3: To develop skills to perform experiments, get familiar with different cutting edge
technologies used to answer research questions and have hands on training on the related

115
 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

area.

CO4: To learn to interpret data, draw conclusion and develop trouble shooting skills.

CO5: To learn to present data, and defend a hypothesis forming the basis of a scientific
study.

Topics Each student has to choose a Principle Investigator depending on his/her research
interest and inclination and has to get involved in any ongoing research project.

Students are required to familiarize themselves with the literature review and scientific
techniques and skills.

Text Reference
Books,
and/or  Related research papers.
reference
material

CO-PO MAPPING:

Course Code: BTS851 Course Title: PROJECT-II

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 3 3 3 2 2 2 3 3 3

CO2 3 2 2 3 2 2 1 1 1 2 3 3

CO3 3 3 3 2 2 2 1 3 3 1 3 3

CO4 3 3 3 2 3 3 2 3 2 2 3 3

CO5 3 3 3 3 3 3 2 3 3 3 3 3

Department of Biotechnology

Course Title of the Program Core Total Number of contact hours Credit

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Code course (PCR) / Lecture Tuto Practical Total

Electives (PCR) (L) rial (P) Hours
(T)

BTS852 PROJECT PCR 0 0 0 0 1

SEMINAR

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment (EA))

All the Program Core EA

subjects

Course CO1: To familiarize developing skills of oration and ability to present an

Outcome analysis/interpretation or conclusion pertaining to biological, clinical and biotechnology
s related research problems.

CO2: To develop presentation skills including making PowerPoint presentation with

proper animation and schema to convince the audience about a hypothesis/ conclusion.

CO3: To develop skills to address scientific questions pertaining to hypothesis, data

interpretation and conclusions.

Topics Each student after completing the project training under a Principle Investigator has to
present the progress/conclusion/interpretation explaining their research project.

Text Reference
Books,
and/or  Related research papers.
reference
material

CO-PO MAPPING:
Course Code: BTS852 Course Title: PROJECT SEMINAR

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 2 3 3 3 3 3 3

CO2 2 1 3 2 2 3 3 3

CO3 3 1 3 2 2 3 2 3 3

Department of Biotechnology

Course Title of the Program Core Total Number of contact hours Credit
Code course (PCR) /
Electives (PCR) Lecture Tuto Practical Total
(L) rial (P) Hours
(T)

BTS853 VIVA PCR 0 0 0 0 1

VOCE

Pre-requisites Course Assessment methods (Continuous (CT) and end assessment (EA))

NA EA

Course CO1: To prepare the students to face future interviews.

Outcome CO2: To develop logical thinking skills in the students.
s

Topics 1. All the topics taught in core courses.

2. Topics taught in the elective courses.
Text NA
Books,
and/or
reference
material

CO-PO MAPPING:

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 SYLLABUS FOR B.TECH PROGRAM IN BIOTECHNOLOGY

Course Code: BTS853 Course Title: VIVA VOCE

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 3 3 3 3 3 3

CO2 3 3 3 3 3 3

119