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Binary Fission in Amoeba

Binary fission and budding are forms of asexual reproduction observed in lower organisms like amoeba and yeast. The document outlines procedures to study binary fission in amoeba using prepared microscope slides. The key steps are placing the slide under the microscope and observing the stages of binary fission, which include the parent cell dividing into two daughter cells after the nucleus divides amitotically and the cytoplasm divides. Budding in yeast is also observed, where a small protrusion forms on the parent cell and a chain of cells is produced after repeated budding and nuclear division.

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Haseeb Rahaman
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3K views2 pages

Binary Fission in Amoeba

Binary fission and budding are forms of asexual reproduction observed in lower organisms like amoeba and yeast. The document outlines procedures to study binary fission in amoeba using prepared microscope slides. The key steps are placing the slide under the microscope and observing the stages of binary fission, which include the parent cell dividing into two daughter cells after the nucleus divides amitotically and the cytoplasm divides. Budding in yeast is also observed, where a small protrusion forms on the parent cell and a chain of cells is produced after repeated budding and nuclear division.

Uploaded by

Haseeb Rahaman
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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AIM

To Study:
a) Binary fission in Amoeba
b) budding in yeast.
with the help of prepared slides.

THEORY

Binary fission and budding are forms of asexual reproduction in lower organisms, like bacteria,
unicellular protozoans, and a few other animals. In binary fission, the parent cell divides into two
daughter cells by amitosis and each daughter cell grows into an adult. The division of nucleus is
called amitosis because the stages of a typical mitotic division are not observed in these cells.
Budding is commonly seen in yeast and Hydra. Hydra is a tiny freshwater organism which produces
young ones from its body laterally yeast, is a unicellular organism which produces a chain of cells
attached to the parent cells

MATERIALS REQUIRED.

A compound microscope, permanent slides of binary fission in Amoeba and budding in yeast.

Figure 1: - Binary fission in amoeba.

Figure 2: - Budding in Yeast.


PROCEDURE

Step 1: The slide is placed under compound microscope


Step 2: The slide is focused under low power and later high power of compound microscope
Step 3: The stages in binary fission and budding are observed carefully

OBSERVATIONS

a) Binary fission in Amoeba

i) In the beginning, the pseudopodia are withdrawn and the body becomes rounded.
ii) Nucleus division amitotically followed by the division of cytoplasm.
iii) A furrow or constriction develops at the point of fission in the main body.
iv) The furrow becomes narrow and finally two daughter cells are formed.

b) Budding in yeast

i) A small out growth or proluberance is seen on the parent cell


ii) Nucleus divides and is later, seen in the bud.
iii) Repeated budding forms a chain of cells.

RESULTS AND DISCUSSIONS.

The prepared slides show asexual reproduction in which only one individual is involved in the
production of new individuals.

PRECAUTIONS.

1. Focus the slides properly.


2 Study the slides first under low-power magnification and then under high-power magnification of
the compound microscope.
3. Draw diagrams as seen under the microscope.

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