Subham vidhyalayaa cbse CHEMISTRY project Exam DA.LS.S.C.E Name : RLHARSHAVARTHINI REGN NO. : CLASS DATE YEAR : 2023-2024 SUBJECT :CHEMISTRY CODE :043Subham vidhyalayaa cbse BONAFIDE CERTIFICATE This bonafide certificate is to certify that Master/Miss Reg No. ..........::1:+-- of class XII has completed his or her project on the topic DETERMINATION OF CAFFEINE IN TEA SAMPLE dforA. INTERNAL EXAMINER EXTERNAL EXAMINER PRINCIPALAcknowledgement | wish my deep sense of gratitude to my Secretary Shri. Mr. ABHAIKUMAR and my gratitude to my PRINCIPAL Mr. F. ANTONY RAJ of my institution for the continuous support and encouragement. a would like to extend ny sincere and heartfett encouragement, the project couldn't have been evolved to be. 1am also thankful to my parents for their cooperation and encouragement.Project is submitted to the Central Board of Secondary Education In fulfilment of the required A.I.S.S.C.E. (2023-2024) BY: Under the guidance of P.G.T. MRS. M. CHITHIRAI SELVI M. Sc., B.Ed., CHEMISTRYow On DH KR W NHN RB mR S CONTENTS TOPICS Introduction Theory Uses of caffeine Effects of caffeine Aim Materials required Procedure Observations Result BibliographyINTRODUCTION Tea is the most commonly and widely used soft beverage in the household. It acts as a stimulant for central nervous system and skeletal muscles. That is why tea removes fatigue, tiredness and headache. It also increases the capacity of thinking. It is also used for lowering body temperature. The principal constituent of tea, which is responsible for all these properties, is the alkaloid-caffeine. The amount of caffeine in tea leavers varies from sample to sample. Originally it was thought that caffeine is responsible for the taste and flavour of tea. But pure caffeine has been found to be a tasteless while substance. Therefore, the taste and flavour of tea is due to some other substance present in it. There is a little doubt that the popularity of the xanthenes beverages depends on their stimulant action, although most people are unaware of any stimulation. The degree to which anindividual is stimulated by given amount of caffeine varies from individual to individual. For example, some people boast their ability to drink several cups of coffee in evening and yet sleep like a (ong, on the other hands there are people who are so sensitive to caffeine that even a single cup of coffee will cause a response boarding on the toxic. The xanthene beverages also create a medical problem. They are dietary of a stimulant of the CNS. Often the physicians face the question whether to deny caffeine containing beverages to patients or not. In fact children are more susceptible than adults to excitation by xanthenes. For this reason, tea and coffee should be excluded from their diet. Even cocoa is of doubtful value. It has a high tannin content may be as high as 50 mg per cup.After all our main stress is on the presence of caffeine in xanthene beverages and so in this project we will study and observe the quantity of caffeine varying in different samples of tea leaves.THEORY The most important methylated alkaloid that occurs naturally is caffeine. Its molecular formula is CgHioNaO2. Its TUPAC name is 1, 3, 7-trimethy(xanthene and common name is 1-methylated thiobromine Purely it is white, crystalline solid in the form of needles. Its melting point is 123°c. It is the main active principle component of tea leaves. It is present in tea leaves up to 3% and can be extracted by first boiling the tea leaves with water which dissolves many glycoside compounds in addition to caffeine. The clear solution is then treated with lead acetate to precipitate the glycoside compounds in the form of lead complex. The clear filtrate is then extracted with extracts caffeine because it is more soluble in it then water.STRUCTURE OF CAFFEINE O x N N xx ; O ’ N O H HN N An | ?USES OF CAFFEINE In medicine, it is used to stimulate, central nervous system and to increase flow of urine. Because of its stimulating effects, caffeine has been used to relieve fatigue. But it is dangerous and one may collapse if not consumes it under certain limit. Caffeine is also used in analgesic tablets, as it is believed to be a pain reliever. It is also beneficial in migraines.EFFECTS OF CAFFEINE POSITIVE EFFECTS It is psycho - stimulant. It improves physical and mental ability. Its effect in learning is doubtful but intellectual performance may improve where it has been used to reduce fatigue or boredom. When administered internally, it stimulates heart and nervous system and also acts as diuretic. “* Caffeine has been shown to increase the metabolic rate.NEGATIVE EFFECTS “+ On the contrary their excessive use is harmful to digestion and their long use leads to mental retardation. “+ Caffeine can increase blood pressure in non-habitual consumers. “+ Caffeine withdrawal can produce headache, fatigue and decreased alertness. “+ High doses of caeffine (300 mg or higher) can cause anxiety.AIM “TO DETERMINE THE PERCENTAGE OF CAFFEINE IN DIFFERENT SAMPLES OF TEA”MATERIALS REQUIRED e TEA LEAVES(RED LABEL TEA, YELLOW LABEL TEA,GREEN LABEL TEA) e BUNSEN BURNER e BEAKERS e LEAD ACETATE e CHLOROFORM e FILTER PAPER e FUNNEL e WATERPROCEDURE 1.First of all, 50 grams of tea leaves were taken as sample and 150 ml of water was added to it ina beaker 2.Then the beaker was heated up to extreme boiling. 3. The solution was filtered and lead acetate was added to the filtrater, leading to the formation of a curdy brown coloured precipitate. 4.We kept on adding lead acetate till no more precipitate has been formed. 5. Again solution was filtered. 6. Now the filtrate so obtained was heated until it had become 50 ml. 7. Then the solution left was allowed to cool.8. After that , 20 ml. of chloroform was added to it. 9.Soon after, two layers appeared in the separating funnel. 10. We separated the lower layer. 11. The solution then exposed to atmosphere in order to allow chloroform to get evaporated. 12. The residue left behind was caffeine. 13. Then we weighed it and recorded the observations. Similar procedure was performed with different samples of tealeaves and quantityof caffeine was observed in them.OBSERVATIONS Weight of china dish = 46.60 gms Weight of china dish with precipitate = 47.20 gms Amount of caffeine = 0.60 gms Weight of china dish= 46.60 gms Weight of china dish with precipitate= 47.15 gms Amount of caffeine= 0.55 gms Weight of china dish= 46.60 gms Weight of china dish with precipitate=47.05 gms Amount of caffeine= 0.45 gmsRESULT 1. Quantity of caffeine in Red label tea is 600mg. /sample of 500 gm. 2. Quantity of caffeine in Yellow label tea is 550mg./sample of 500 gm. 3. Quantity of caffeine in Green label tea is 450mg./sample of 500 gm.Graphically plotting various tea samples in accordance with the amount of caffeine present in them we present a stunning find: Catton contort in diferent ea samples, Order of quantities of caffeine in different samples of tea leaves: RED LABEL >GREEN LABEL