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Preparation

The document provides instructions for preparing several reagents including glycerol jelly, caffeine benzoate, lactophenol solution, tris succinate buffer, alkaline cupric tartrate solution, Benedict's reagent, Bayer's stock solution, and fluoride oxalate reagent. The reagents require various chemicals and precise measurements to be mixed with distilled water.

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Nermin Elsayed
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48 views2 pages

Preparation

The document provides instructions for preparing several reagents including glycerol jelly, caffeine benzoate, lactophenol solution, tris succinate buffer, alkaline cupric tartrate solution, Benedict's reagent, Bayer's stock solution, and fluoride oxalate reagent. The reagents require various chemicals and precise measurements to be mixed with distilled water.

Uploaded by

Nermin Elsayed
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Preparation of :

A- Glycerol jelly reagent


1- gelatin 15 g
2- glycerol 50 ml
3- distilled water 250 ml. F- Benedict reagent.
1- copper sulphate 8.6 g
B- Caffeine benzoate reagent 2- sodium carbonate 50 g
1- Sodium acetate 3- tri sodium citrate 86.4 gm
2- sodium benzoate 28 g 4- distilled water to 500 ml
3- EDTA 0.5 g
4- distilled water 500ml. G- Bayer's stock solution
1- copper chloride dihydrate 0.7g
C- Lactophenol solution. 2- acetic acid-glycial 1 ml
1- phenol 25 g. 3- distilled water to 107 ml
2- lactic acid 25 g
3- glycerol 50 ml H- Floride oxalate reagent
4- distilled water 250 ml 1- Sodium floride 1g
2- potassium oxalate 3g
D- Tris succinate buffer. 3- distilled water 100 ml
1- succnic acid 5.6 g
2- sodium hydroxide 1.0 g
3- sodium azide 0.1 g
4- distilled water to 1L

E- Alkaline cupric tartrate solution


1- potassium sodium tartarte 35.0 gm
2- sodium hydroxide 7.5 gm
3- distilled water to 100 ml

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