CHAPTER I
THE PROBLEM AND ITS BACKGROUND
Background of the Study
Sugar is required for the healthy functioning of
our body. It serves as the primary source of energy for
cells in our body, especially for the brain. The brain
requires enough glucose to function for cognition,
memory, and understanding. Glucose is made by our body
through breaking down the food molecules such as
carbohydrates, proteins, and fats. Moreover, sugar
occurs naturally in all foods containing carbohydrates
including fruits, vegetables, grains, and dairy
products.
On the other hand, sucrose is a disaccharide
composed of the monosaccharide’s glucose and fructose.
It is frequently referred to as "table sugar”. Depending
on its origin, sucrose can be a natural or added sugar.
When derived directly from whole plant sources, it is
termed a natural sugar. When consumed in packaged foods
and beverages to which sucrose has been added during
production, sucrose is considered added sugar.
Commercial sugars or table sugar commonly come from
sugarcane. Sugarcanes were extracted and processed to
1
produce granules or crystalized sugar which are then
used to sweeten foods, drinks, and even fruit. Aside
from the sweetness being the primary reason for using
sugar, it has numerous applications too. It can be used
for preserving, fermenting, texture modifier,
flavoring, and etc. This kind of sugar is produced from
fruits commonly from sugarcane, sugar beets, sugar
palms, and sorghum. These fruits contain sucrose and
also the Muntingia calabura.
The common foods we eat everyday such as fruits,
vegetables, whole grains, and mill naturally has sugar.
Health related problems regarding to sugar only exist
when we consume excess or added sugars. Sugarcane and
beet sugar are the common sources for table
(conventional) sugar or the refined sugar. On the other
hand, there are natural alternatives to fined sugar.
These are sugar alcohols: erythritol and xylitol that
are derived from plants; stevia, a plant leaf extract
that is hundred times sweetener than conventional sugar;
agave, a plant nectar that has high fructose content;
coconut sap sugar; date sugar; monk fruit; fruit puree;
honey; maple syrup; brown rice syrup; and blackstrap
molasses. All in all, natural sweeteners or the
2
alternatives are considered heathier than conventional
sugar because they include more vitamins, minerals,
antioxidants, and other bioactive compounds that may be
beneficial. However, just because they contain more
nutrients than regular sugar does not indicate that they
should be consumed on a regular basis. Because most of
these sweeteners contain other components, it's vital
to use caution (Traxler, 2023).
Furthermore, in the Philippines, there are various
sweet fruits and we can obtain alternative sugar from
these resources. There were more than 300 edible fruit
bearing species in the country. Fruit trees of high
value can now be grown profitably in Luzon and
elsewhere. Rambutan, durian, lanzones, mangosteen,
longan, lychee, pitaya, and atemoya orchard growers are
running thriving businesses. According to the
proceedings of the Philippine Fruit Association's 16th
Annual National Fruit Symposium, these fruit trees were
once plentiful in Mindanao and Vizayas, but have
recently gained popularity, adaptation, and
productivity in Northern Luzon, Philippines (Miranda et
al., 2018).
3
As mentioned, there are abundant amount of fruit
bearing trees in the Philippines and Muntingia calabura
or commonly known as Aratiles in the country is one of
those. Aratiles is a fruit native to Central America,
in tropical nations such as Brazil, China, India,
Malaysia, and the Philippines, owing to its excellent
soil and climate adaptation (Mahmood, et al., 2014).
Aratiles has variety of sources in different parts
of the world, including Southeast Asia. In India,
Aratiles tree is commonly grown in urban gardens and
parking lots. In Mexico, Aratiles fruits are sold in
the markets. In Brazil, Aratiles is planted in
riverbanks to lure fishes for the fishermen to catch.
On the other hand, because of the quantity of the
fruits, especially during the fruiting season, Aratiles
is not sold in markets in Indonesia, where the fruits
are usually consumed raw as well as here in the
Philippines.
According to Philippine Medicinal Plants, Aratiles
is a fast-growing tree with spreading branches that
grows 5 to 10 meters tall. Hairy, sticky, alternate,
distichous, oblong-ovate to broadly oblong-lanceolate,
8 to 13 centimeters long, with toothed margins, pointed
4
apex, and inequilateral base, one side rounded, the
other acute. Flowers are white, extra-axillary,
solitary or in pairs, and about 2 centimeters in
diameter. The sepals are 5 in number, green, reflexed,
lanceolate, and about 1 centimeter long. Petals are
white, obovate, and 1 centimeter long. They are
deciduous and spread. The fruit is a berry that is
rounded, about 1.5 centimeters in diameter, red when
ripe, smooth, fleshy, sweet, and has many seeds. The
ripe fruit is a favorite of Filipino children. Fruits
are processed into jam, and leaves are used to make tea.
Folkloric uses include - flower decoction for abdominal
cramps. As an emollient, the decoction is used. Flowers
have been used as an antiseptic and to treat spasms.
The leaves are used as an antiseptic or antipruritic,
as well as to treat abdominal cramps. It is also used
to treat colds and headaches.
The antioxidant activity of calabura fruit, as well
as its soluble carbohydrates, volatile and phenolic
compounds, was investigated. The calabura berry is
classified as low-FODMAP due to its low amount of
fermentable oligo-, di-, and monosaccharides and
polyols (FODMAPs) and similar amount of glucose and
5
fructose. The major volatile components were the
terpenes -Farnesene and dendrolasin identified by SPME-
GC-MS. Gallic acid (5325 g/g dw) and cyanidin-3-O-
glucoside (171 g/g dw) were found to be the most
abundant phenolic compounds, followed by gentisic acid,
gallocatechin, caffeic acid, and protocatechuic acid.
Gallic acid was also found primarily in esterified (2883
g/g dw) and insoluble-bound (2272 g/g dw) forms. The
presence of flavonoids (0.28-27 g/g dw) in these
fractions, such as catechin, gallocatechin,
epigallocatechin, naringenin, and quercetin, resulted
in the highest antioxidant activity in free and
glycosylated forms. These findings clearly indicate
that calabura is a low-energy berry with an appealing
color and flavor that may contribute to the intake of
several bioactive compounds with antioxidant activity.
This berry also has a lot of potential for use in the
food industry and as a functional food (Gustavo,
Henrique, Damila, Marcos, & Glaucia, 2018).
6
Taxonomical Classification
Kingdom Plantae – Plants
Class – Magnoliopsida
Superorder – Rosanae
Order – Malvales
Family – Muntingiaceae
Genus – Muntingia L.
This study aimed to develop alternative sweetener
from Aratiles fruit that is acceptable at two different
treatments. Specifically, it aimed to determine the
level of evaluation of Aratiles sugar compared to
commercial sugar in terms of appearance and preference
to use; to determine the level of evaluation of the
developed sugar based on sensory evaluation regarding
its taste, aroma, and texture; and to test the shelf
stability of Aratiles sugar.
Statement of the Problem
1. What are the sensory evaluation qualities given to
Aratiles sugar – ripe and combination of ripe and
unripe as alternative sweetener in terms of:
a. Taste
b. Aroma
c. Texture
7
2. What are the sensory evaluation qualities given to
Aratiles sugar (ripe and combination of ripe and
unripe) and commercial brown sugar in terms of:
a. Appearance
b. Preference
3. Is there a difference between the population mean
of evaluation score in terms of taste, aroma, and
texture of sweetener made from Aratiles fruit
extract (pure ripe, and combination of ripe and
unripe) to the proposed value of population mean
which is 1.0?
4. How are the Aratiles sugar – pure ripe Aratiles
sugar and combination of ripe and unripe Aratiles
sugar in terms of shelf stability?
Significance of the Study
The results of the study will be a great benefit
to the following group of people.
Consumer. The study benefits individuals who desire to
consume sweetener but a means to the less additives in
terms of its content.
Product Developers. This study will provide an overview
about the Aratiles as an alternative sugar to the
8
potential product developers to enhance and to discover
improvements.
Food Technology Students. This study would help food
tech. students as a source of guide for their future
experimental research. It can be a guide to develop new
recipe in simple way.
Future Researchers. The researchers will have a chance
to develop further ideas and will serve as their
references to their related studies.
Hypothesis
Ho
There is no significant difference between
Aratiles alternative sweetener and commercial sugar in
terms of appearance and preference.
Scope and Limitation of the Study
The general intent of this study is to determine
if Aratiles can be a potential source to produce sugar
cubes.
This study is limited on the production of sugar
from Aratiles fruit extract only. It mainly identified
and assessed if there are significant differences
9
between sugar from Aratiles and commercial sugar in
terms of appearance and preference.
In this study, parents and faculty of School of
Our Lady of Atocha Inc., are covered as part of the
respondents. Questionnaires with quantifiable choices
are distributed and the data were analyzed.
Definition of Terms
The following terms were defined conceptually.
1. Acceptability – The satisfactory by virtue of
conforming to approved standards.
2. Appearance – It is the way something looks.
3. Aratiles – A fast-growing tree of disturbed lowland
neotropical forests that has been introduced as an
ornamental and fruit tree in many Old World
countries.
4. Aroma – It is a distinctive, typically pleasant
smell.
In this study, it is how the respondents describe
(thorough scale) the smell of the Aratiles sugar.
5. Commercial Sugar – Sweet crystalline substance
obtained from various plants, especially sugar
10
cane and sugar beet, consisting essentially of
sucrose, and used as a sweetener in food and drink.
6. Preference – It is a greater liking for one
alternative over another or others.
7. Shelf stability - It is the ability of the food to
be stored at room temperature for an extended
period of time before going bad.
8. Sugar cubes – It is a small cube of compacted sugar
used for sweetening hot drinks usually.
9. Taste – It is the sensation of flavor perceived by
the mouth
10. Texture – In food, those are properties of
food that are sensed by touch in the mouth or with
the hands.
Theoretical Framework
Theories Related to Acceptability of a Food Product
Consumers seek food with specific sensory
characteristics, so sensory quality should be
considered a key factor in food acceptance. The
acceptance of the food will be determined by how well
it responds to consumer needs and the level of
11
satisfaction it can provide. Acceptance of a food is
essentially the result of an interaction between food
and man at a certain moment, food characteristics
(chemical and nutritional composition, physical
structure and properties) the influence of consumers
decision to accept or reject a food (Shepherd & Sparks,
1994).
Disconfirmation Theory
According to this theory, "Among the most popular
Satisfaction Theories" is the disconfirmation theory,
which argues that satisfaction is related to the size
and direction of the disconfirmation experience that
occurs as a result of comparing service performance to
satisfaction. In an elastic sense, satisfaction is the
result of direct experiences start with products or
services, and it occurs when perception is compared to
a standard (Mattle & Veill, 2003).
Theory of Planned Behavior (TPB)
The TPB is a theory that explains the psychological
components of decision-making and posits that people
make rational decisions to engage in specific acts. The
12
TPB believes that behavioral intention, which
represents the motivations and cognitive planning for
engaging in such behavior, predicts consumer behavior.
Attitude, subjective norm, and perceived behavioral
control, in turn, all influence intention. Attitude is
related to assessing the positive and bad results of
behavior. A subjective norm is a perception of societal
expectations for behavior. Finally, perceived
behavioral control is related to a person's perception
of his or her ability to engage in the behavior (Ajzen,
1991).
Previous research on food choice found that
attitude and perceived behavioral control are important
predictors of people's intentions, but the predictive
power of subjective norm is less consistent (Kumar &
Smith, 2018; Qi & Ploeger, 2019;Wang et al., 2016). On
the one hand, subjective norm is one of the weakest
antecedents of intention, according to several meta-
analyses (Armitage & Conner, 2001). Many empirical
investigations, on the other hand, have proved its
importance in understanding intention (Rezai et a.,
2017).
13
Value-Belief-Norms Theory (VBN)
According to VBN theory, moral norms are engaged
when an individual is aware of the results of her
activities and is willing to accept responsibility for
these outcomes. This theory is operationalized by
measuring behavioral intention as indicated by the
personal norm, which is the awareness of an obligation
to conduct in accordance with our moral convictions.
Personal norm, in turn, is explained by ascription of
responsibility, which is an individual's sense of
obligation to the environment. Furthermore,
accountability is defined by understanding of the
consequences of human activities, which is derived from
basic pro-environmental ideas (Stern, 2008). The VBN
factors have already been proven in the literature on
the antecedents of food choice to explain sustainable
food choices.
The Additional Role of Trust
According to (Carfora et al., 2021), the trust of
consumers is particularly relevant to the current study
because consumers have limited knowledge about natural
14
foods and are rarely exposed to the production or
preparation of this category of food (Hartmann et al.,
2015). Recent studies have confirmed that trust in the
food supply chain is a significant predictor of
consumers' intent to purchase food products within the
TPB application (Menozzi et al., 2017).
The above-mentioned theories would be used in this
study to assess one's behavior in terms of how they will
accept the experiment product in terms of taste, aroma,
texture, appearance, and preference. Direct experience
is thought to influence one's behavior and perception.
15
Research Paradigm
INPUT PROCESS OUTPUT
• Ripe Aratiles • Harvesting • Acceptability
fruit • Washing of Aratiles
• Combination • Extracting sugar as
of Ripe an • Boiling alternative
Unripe sweetener
• Molding
Aratiles
fruit • Freezing
• Evaluation
• Baking powder
FEEDBACK
Figure 1. Research Paradigm
The paradigm above explains the input, process,
output and outcome of the study.
The input consists of the ingredients and materials
that will be used in the experiment. The process is
about the experimentation in making sugar out of
Aratiles. The process includes harvesting, washing,
extracting, boiling, molding, freezing, and sensory
evaluation. Data gathering is through questionnaires
and the gathered information will be analyzed and
16
interpreted. The output of the study will be the
acceptability of Aratiles as alternative sweetener in
the form of sugar cubes.
17
CHAPTER II
REVIEW OF RELATED LITERATURE AND STUDIES
This chapter presented the summary of all the
reviews from various foreign and local studies and
literature that tackle the different ideas, concepts,
generalizations and conclusions related to our present
study. This served as a guide to the researchers in
developing the study. This chapter also helped the
researchers to familiarize the information that are
relevant and similar to the present study.
Related Foreign Literature
Our bodies need one type of sugar to survive,
called glucose (NIH News in Health, 2014). Sugar alone
is not bad in our bodies; it only causes negative
impacts because of too much consumption. It is added in
our food and beverages to make better tastes. However,
these added sugars can be high in calories unlike the
fruits, which are naturally sweet foods. There are
artificial sweeteners that are low-calorie but it can
cause sweet tooth that leads to overeating (NIH News in
Health, 2014).
18
Sugar can be derived from a variety of sources,
including sugar palms, sugar beets, and fruits, but the
white granulated product we are acquainted with today,
which represents the pinnacle of technological
achievement in sugar processing, is made from sugar cane
and sugar beet (Mintz, 2013). It was cane sugar that
introduced the Europeans to the commodity we now call
sugar. Although we know sugar cane was farmed in South
Asia as early as the fourth century B.C., definitive
evidence of processing—boiling, clarifying, and
crystallization—does not exist until nearly a
millennium later. It is concluded that studies of the
everyday in modern life, of the changing character of
such mundane matters as food, viewed through the
perspective of production and consumption, use and
function, and concerned with the emergence and variation
of meaning, could be one approach to reviving a
discipline that is dangerously close to losing its
purpose.
There are ten types of sugar with their best uses.
These are white sugar, brown sugar, caster sugar,
confectioners’ sugar, cane sugar, muscovado sugar,
demerara sugar, coconut sugar, turbinado sugar, and
19
liquid sugar (Finedining Lovers, 2023). One of the types
of white sugar is the sugar cubes. It is sugar pressed
into cube shape and usually used for hot drinks.
However, in the current study, sugar cubes from Aratiles
fruit were not pressed sugar. Instead, it is molded into
cubes through square-shaped molder that has 1ml in each
cube, and placed into the freezer to form cubes.
The negative effects of additional sugar are
becoming more apparent and people are turning to natural
alternatives as a result (Gunnars, 2018). The author of
this literature aims to separate reality from fiction
in order to ascertain whether coconut sugar is actually
a healthy sugar substitute. He used a comparative
analysis as a method of comparing regular sugar to
coconut sugar in terms of its mineral content and their
glycemic index. It is concluded that coconut sugar has
small amounts of minerals, antioxidants, and fiber. It's
fairly similar to conventional table sugar, but it's
less refined and includes less nutrients. It is
suggested that if you're going to use coconut sugar, go
easy on it. This literature adds up to our study by
giving an idea that a fruit (coconut) can be a potential
healthy sugar substitute.
20
The findings of this article suggests that cabalura
fruit with low energy value can contribute to the intake
of several bioactive (Pereira et al., 2018). This study
shows the components of Aratiles and it could have the
possibilities for food applications. Therefore, as the
compounds being assessed by the author of this study,
it will provide information about the compounds that
Aratiles have which helps into proving the it could be
a source of alternative sweetener.
Synthesis
The above collection of foreign literature is
related to the development of alternative sweetener out
of Aratiles. It discusses what is sugar, its different
sources and ways of producing, and the various types of
sugar. Sugar substitute were also stated and coconut
sugar is an example. It serves as basis of the
researchers that a fruit, the Aratiles fruit to be
specific, has potential as source of alternative
sweetener.
21
Related Local Literature
The term 'Intense sweeteners' (IS) refers to
various substances of plant origin or obtained by
chemical synthesis, used in the food industry for their
high sweetening power and their low caloric value
(Bruyere, Ahmed, Atlan, Belagaud, Bortolotti, Lavier,
Charriere, Girardet, Houdart, Kalonji, Nadaud, Rajas,
Slama, Margaritis, 2015). The purpose of this research
is to examine the advantages of alternative sweeteners
The study's goal was to find both published and
unpublished studies. In terms of nutritional benefits,
the available studies, while numerous, do not provide
evidence that the use of artificial sweeteners as sugar
substitutes benefits weight management, blood glucose
regulation in diabetic subjects, or the incidence of
type 2 diabetes.
Filipino cuisine, from appetizer to dessert,
displays the country's typical fondness for sweets.
While practically every ingredient, including sugar, is
readily available on shop shelves nowadays, nothing
beats natural or unprocessed foods (Medenilla, 2022).
This literature discusses some of the traditional
sweeteners in the Philippines conducted by John Sherwin
22
S. Felix. First is the balikucha made from pure sugar
cane syrup. It is an alternative to refined sugar and
is a palmier-shaped confection known in the Ilocos
region. Second is the muscovado sugar, a pure and
unrefined sugarcane. “It is raw sugar obtained from the
juice of the sugarcane by evaporation and draining off
molasses”, defined by Merriam-Webster. Third
traditional sugar is the pakaskas made from the sap of
the Buri palm tree. Fourth is the panutsa de bao , a
sugar cane chunk with a shape of coconut shell or “bao”.
Therefore, this literature states that as more customers
trend toward adopting healthier food options, it is
hoped that the manufacturing and consumption of these
traditional sweets or sweeteners will continue.
Consumers are particularly focused on picking
goods with clean labelling, natural components,
preferably with extra functional features, without the
loss of taste (Saraiva et al., 2020). Their study
provides details about perceptions and attitudes of
consumers to natural foods and it is focused on natural
sweeteners. People often choose the ‘healthier’ options
or the foods that are made naturally, like in
sweeteners. Their supports the current study with the
23
information regarding the consumers’ preferences.
However, it should be noted that a long tradition of
use in some restricted societies and areas around the
world, while providing some reassurance, does not
eliminate the need for detailed scientific studies to
prove the safety of natural compounds to be used as food
additives and, sweeteners (Saraiva et al., 2020).
Synthesis
The collection of local literatures that was just
discussed offers details about the Aratiles fruit,
including its constituents and the positive effects it
has on one's health. The collection above also includes
discussions on sweeteners with a high intensity level,
sweeteners with low calories, traditional sweeteners
from the Philippines, and the consumers; perceptions an
attitude toward natural product- sweeteners.
Related Foreign Studies
The fruit of the common shade tree Muntingia
calabura, known as Kersen or Cherry, is not yet
advertised as a consumable fruit or a nutritious herbal
beverage (Niwele et al., 2020). Researchers examined
into whether the fruit juice and extracts from the fruit
pulp of M. calabura could decrease the activity of
24
certain metabolic disease-related enzymes, including a-
glucosidase, a-amylase, xanthine oxidase, lipase, and
protease as well as the juice's and the extract's
antioxidant capacity. The study is done through
preparation of fruit juice and crude extract of fruit
pulp and was evaluated for inhibitory activities against
α-glucosidase, α-amylase, xanthine oxidase, lipase, and
protease. The researchers suggested that the fruit of
M. calabura is a potential source of antidiabetic and
anti-hyperuricemia agents and can be further explored
for nutraceutical applications. Therefore, this study
helps the researchers of the present study to determine
the properties of M. calabura or Aratiles fruit extract
to conclude if it is safe to produce sugar cubes from
it.
Traditional brown sugar making includes the
concept of heat transfer in the process (Sholihah et
al., 2021). The researchers of this study made
qualitative research to explore and identify the ideas
and wisdom about making brown sugar that the locals in
the area of study have. The data in this study were
gathered through interviews, documentation, and direct
observation. Field observations were done where the
25
traditional brown sugar production was directly
observed. Therefore, it was concluded that there is a
concept of physics (heat transfer) in the process of
making traditional brown sugar or Ndewan processes which
is the conduction, convection, and radiation. In
conduction, the process occurs in the heated pan where
the sap in cooked using an aluminum skillet. This
process happened because of the molecular collisions
that occur when objects are heated (Douglas, 2014). In
convection, the locals use srumbung to limit the
movement of heat transfer. In this process, convection
occurs because of the heat that flows through the mass
movement of molecules from one place to another. In
radiation, the process occurs in the study through the
transfer of heat from the flame to their body. It is
the heat transfer that happens without things touching
each other because it emits energy in the form of
photons. This recent study provides insight into how a
fruit extract (coconut sap) can be processed
traditionally to produce a brown sugar through heat
transfer.
Because of their nutraceutical characteristics,
alternatives such as unprocessed sugars have generated
26
a lot of attention as a healthy substitute (Arshad et
al., 2022). This research study aims to discuss the
health benefits of sugar generated from natural sources
and to highlight health issues that may be induced by
refined processed sugar. Although refined sugar is
commonly used in a variety of products such as processed
foods, soft drinks, and ice creams, it is considered
unhealthy due to its high salt and sugar content, as
well as added fats and artificial coloring. Natural
sugars are recommended because they have a high
nutritional value as well as a high concentration of
beneficial chemicals that counteract the negative
effects of refined sugar. As a result, eliminating or
limiting refined sugar consumption should be advocated
as a better option in food choices. Thus, this study
helps us to have a basis on producing alternative
sweeteners from a natural source (Aratiles fruit).
This study identified critical characteristics of
protein beverages and investigated the effects of
priming on protein beverage enjoyment. Label claim,
protein type, protein amount, carbohydrates,
sweeteners, and metabolic benefits were all considered.
Level utility scores and attribute importance ratings
27
were calculated. Whey protein beverages with naturally
sweetened, reduced sugar, and 15 g protein per serving
were the most popular. Three consumer clusters were
found based on their protein kind, sweetener, and
protein amount preferences. Priming remarks influenced
concept liking positively (P 0.05), but had no effect
on total like (P > 0.05). Consumers preferred beverages
with 10 g protein over beverages with 20 g protein (6.8
vs. 5.7, P 0.05), which was consistent with trained
panel profiles of enhanced cardboard flavor with higher
protein content. All in all, in this study, protein
beverages must have palatable flavors in order to appeal
to a wide range of consumers (Oltman et al., 2015).
Chocolate milk enhances children's milk
consumption, but its high sugar content raises health
concerns. Sugar reduction interest and parents' desire
for natural sweeteners need additional research on
natural nonnutritive sweeteners. However, it is
critical to maintain consumer acceptance, particularly
among children, while reducing sugar in chocolate milk.
In this study, young adults found chocolate milks
sweetened purely with nonnutritive sweeteners to be less
appealing than SUC controls. Young adults and children
28
approved of Monk fruit/MK25 and Stevia leaf/STV25. The
display of information on chocolate milk labels had
varying effects on parental acceptance. Skim chocolate
milk/SCM sweetened with sucrose was chosen by
traditional parents, while SCM sweetened with natural
nonnutritive sweeteners was preferred by label-
conscious parents (Li etal., 2015).
The given research supports the idea that Mouth
Behavior influences food texture choice and
preferences. When offered a wide range of food options
to choose from during qualitative research, there were
evident tactile disparities between Mouth Behavior
groups in food items identified as ''love" or ''Not
worth buying." The textures picked as ''liked" were
those whose texture most closely suited their Mouth
Behavior (could be easily consumed with their desired
Mouth Behavior); whereas items rejected had textures
that made them difficult to consume with their primary
Mouth Behavior. Individuals attempted to modify the
product into a palatable texture. As a result, the
texture of a given dish was interpreted differently by
each group. This texture, according to study, is not
static and fluctuates during the eating process the
29
manner in which the texture changes is crucial in
influencing food product acceptance (Jeltema et al.,
2016).
While the majority of customers believe they are
not readily duped, their sense of taste is frequently
tricked by their sense of sight. This is because
everyone has their own ideas about how certain things
should look. When the color of food differs from what
the consumer expects, the consumer expects the meal to
taste differently. Consumers utilize visual signals to
determine the quality of food they intend to eat
(Endrizzi et al., 2015)
The process through which an individual accepts or
rejects food is thought to be multidimensional. Food
acceptance is determined by three essential aspects.
They consist of customer attributes, sensory qualities,
and food enjoyment. Food sensory features such as
flavor, texture, aroma, and appearance all have distinct
and powerful influence on food acceptability. As a
result, a sensory property of food is regarded as the
critical area in which food manufacturers can
successfully differentiate their products. Knowledge,
innovativeness, attitude, belief, and perception of
30
specific food products are all consumer attributes that
influence food acceptability. Finally, the 'feel good'
element is an important determinant of food
acceptability (Maina, 2018).
Synthesis
The above collection of foreign studies explains
how raw materials, specifically sugarcane, are
manufactured and the traditional method of producing
sugar. It will provide researchers with information on
how to process Aratiles to make sugar. Furthermore, the
mentioned studies discuss Aratiles fruit extract as a
potential source of anti-diabetic and antioxidants.
Related Local Studies
Recent nutritional research has focused on the
antioxidant capacity of foods, but current dietary
recommendations aim to increase intake of antioxidant
foods rather than supplementing with specific
nutrients. Many substitutes for refined sugar are
available and include raw sugar, vegetable
juices/syrups (such as maple syrup and agave syrup),
molasses, honey and fructose (such as date sugar).
Similar to whole grains versus refined grains, it has
31
been hypothesized that unrefined sweeteners contain
higher levels of antioxidants (Phillips, 2013). To
compare the total antioxidant content of natural
sweeteners as alternatives to refined sugar.
Substantial differences in total antioxidant content of
different sweeteners were found.
Coconut sugar is traditionally produced by
evaporating sap until reaching its saturated liquid and
forms a crystalline structure (Sugandi, 2018). This
study investigated the comparison of coconut sugar made
by traditional method (crystalline structure) and dried
coconut sugar (predominantly amorphous structure) to
its characteristics. Coconut sap is a nutritious
material due to its composition and easily fermented
and deteriorated during collection. Traditionally, the
farmers usually add preservatives (natural or chemical
preservatives) and lime (Ca(OH)2) to maintain the
stability of coconut sap during further processing.
Traditional coconut sugar powder has a dominant
crystalline structure while dried ones have amorphous
structure. This study will help us to better understand
the properties of crystalline and amorphous coconut
sugar produced by different processes. Such information
32
can be used as an alternative method to produce coconut
sugar powder with desirable properties.
Coconut sugar industry needs a marketing plan that
will attract more consumers to the market (Mendoza and
Cruz, 2019). A study conducted by the researchers at
the Philippines aims to determine the long term demand
for coco sugar, as well as identity factors influencing
its supply chain. The author proposes a new model of
production and management that would accommodate all
current and potential demand for this product. Survey
interviews with key supply chain players were conducted
to establish a foundation for demand modeling and
forecasting methods. However, this survey interview
identifies that coconut sugar can be a great substitute
for regular refined and /or muscovado sugar due to the
promising market on diabetic prevalence . Coconut sugar
is a sustainable product because of its high market
potential and growth because of the health benefits it
provides. It has been determined that coconut sugar
should be used as a low-risk alternative for diabetics.
This related local study serves as a guide for the
researchers, assures that the Aratiles will be a good
alternative sweetener.
33
To determine the stability of the raw sugar and
the effects of prolonged storage in different
warehousing conditions or location (Sumagaysay, 2019).
To determine the stability of the raw sugar, samples
were drawn initially and quarterly thereafter and
analyzed for Polarization, Moisture, Color, Ash and
Reducing Sugar. Statistical evaluation of the results
of the analysis using Two Way Analysis of Variance and
Linear Correlation showed the relationship between the
analytical variables and the independent variables such
as mill/location and time/quarter. From the statistical
analysis, time was shown to be significant factor as to
change in all analytical parameters analyzed.
Polarization, moisture, color, ash was significantly
different among mills while among mills differences in
values on reducing sugar are not significant. As seen
from the results, raw sugar quality parameters after
prolonged storage of two years differ significantly and
have means that are not the same.
34
Synthesis
The above collection of local studies provides
information on the techniques used to produce high-
quality sugar. It also discusses the possibility of
using coconut sap to produce traditional sugar. Thus,
these studies will supplement the current research by
providing information about sugar production.
35
CHAPTER III
METHODOLOGY
This chapter presented the research methodology
used by this study which includes the research design,
research procedure, participants, research instrument,
scoring and interpretation, data gathering procedure,
and the statistical treatment of data.
Research Design
This study used experimental design. Ripe and
combination of ripe and unripe Aratiles fruits are the
factors of this study. Using statistics, true
experimental research design tests the researchers'
hypothesis that sweetener made from Aratiles fruit
extract (pure ripe, and combination of ripe and unripe)
has no significant difference to commercial sugar.
The design is best for this research because
researchers experimented on specific variables to draw
conclusions. Surveys with scalable answers are used in
this research.
36
Research Procedure
I. In making sugar from Aratiles, this method is
followed:
PROCEDURE
Harvesting Washing Extracting
Freezing Molding Boiling
Evaluation
1. Preparation is done through harvesting Aratiles
fruits. There are group of harvested fruit only
consist of ripe Aratiles and the other group is
combination of ripe and unripe.
2. The Aratiles were washed through rinsing with water
to remove dirt from the surface.
3. Rinsed fruits were extracted through smashing it
manually and separating the peel and pulp from the
juice through straining.
4. The strained juice of Aratiles was boiled over
medium heat using pan. This step was done to remove
any impurities from the juice.
37
5. Next step was the pouring of boiled juice in the
molder for cubes that has 1 ml each.
6. After step number 5, the poured juice in the molder
was placed in the freezer.
II. The Aratiles sugar treatments were tested for
sensory evaluation in terms of appearance end preference
compared to commercial brown sugar.
The testing procedure for sensory evaluation.
1. Appearance and preference were evaluated for each
of the treatments (ripe Aratiles sugar,
combination of ripe and unripe Aratiles sugar)
against commercial brown sugar.
2. The researchers gathered data through
questionnaire with five-point scale, from
respondents.
3. The results were recorded and evaluated.
III. The Aratiles sugar are tested for sensory
evaluation in terms of taste, aroma, and texture.
The testing procedure for sensory evaluation
38
1. Taste, aroma, texture was evaluated for each of
the treatments (ripe Aratiles sugar, combination
of ripe and unripe Aratiles sugar).
2. The researchers gathered data through
questionnaire with five-point scale, from
respondents.
3. The results were recorded and evaluated.
IV. The Aratiles sugar treatments are tested for shelf
stability.
1. Appearance was tested daily through
observation.
2. The results were recorded and evaluated.
Participants
The participants of the study were composed of 37
respondents from SOLA faculty and staffs and parents,
randomly evaluated. The 37 respondents were derived from
random sampling. The population size is 75, confidence
level is 90%, and margin of error is 10%, thus the
sample size is 37.
39
Research Instrument
For this research, a questionnaire was used as main
instrument to gather the needed data. In validity the
questionnaire was responded using five-point hedonic
scale. Hedonic Scale is a scale that indicates the
extent to which respondents like or dislike something,
such as a product they tasted or a concept they observed
(Esomar, 2016).
Scoring and Interpretation
To determine the acceptability of the product based
on taste, aroma, texture, appearance, and preference,
the researchers used five-point hedonic scale. The score
ranges from 1 to 5 and summed up and computed to find
weighted mean and identify its rating form.
Scale Ranges of Description
Scores
1 1.00 – 1.49 Dislike
2 1.50 – 2.49 Neither
Like/Dislike
3 2.50 – 3.49 Like Slightly
4 3.50 – 4.49 Like Moderately
5 4. 50 – 5.00 Like Very Much
40
Data Gathering Procedure
There are two (2) treatments: Ripe Aratiles Sugar
and Combination of Ripe and Unripe Aratiles Sugar. T1,
T2, and commercial sugar in terms of appearance and
preference, were evaluated. The disposable spoons were
color coded according to what treatment the respondent
is evaluating. The same gathering procedure is also done
with the evaluation of taste, aroma, texture with T1
and T2.
The data gathered for the evaluations were
retrieved and subjected to data analysis.
Statistical Treatment of Data
Weighted Mean
The means for Sugar A, Sugar B, in terms of taste,
aroma, and texture and Sugar A, Sugar B, Sugar C, in
terms of appearance and preference, is calculated using
the following formula.
𝑭
Formula: 𝑷 = 𝑯 × 𝟏𝟎𝟎
Where:
P – percentage
F – frequency
41
N – number of cases
100 – constant
The data gathered from evaluation is analyzed using two-
tailed One-sample T-test.
where
where
μ0 = The test value - the proposed constant for the
population mean
x¯ = Sample mean
n = Sample size (i.e., number of observations)
s = Sample standard deviation
sx¯ = Estimated standard error of the mean (s/sqrt(n))
The calculated t value is then compared to the
critical t value from the t distribution table with
degrees of freedom df = n - 1 and chosen confidence
level. If the calculated t value > critical t value,
then we reject the null hypothesis.
42
CHAPTER IV
PRESENTATION, ANALYSIS, AND INTERPRETATION OF DATA
This chapter presented the data gathered, the
results of the statistical analysis done and
interpretation of findings. These were presented in
tables following the sequence of the specific research
problem regarding the Muntingia calabura (Aratiles) as
an Alternative Sweetener.
43
TABLE 1.1: The Measurements of Treatment 1 – Ripe
Aratiles sugar
Treatment 1 Ripe Aratiles Trial 1 Trial 2 Trial 3
Sugar
Mass of Aratiles (g) 250g 87.5g 750g
Temperature (°C) 100°C 100°C 70°C
(oven) (oven) (stove)
Temperature (°C) (Boiling N/A N/A 100°C
point)
Time (s) 40s 90s 600s
Volume (L) 0.25L 0.0875L 0.235L
Number of cubes N/A N/A 160 cubes
Amount of Baking Powder N/A N/A 5.69g
Storage Temperature (°C) N/A N/A 2.4°C
Temperature (°C) N/A N/A 6.7°C
The table shows the measurements of trial 1, trial
2, and trial 3 of treatment 1 (ripe Aratiles). In trial
1, before the juice extraction of ripe Aratiles fruit,
44
it weighs 250g. Then, the extracted juice was 0.25L and
spread into the baking paper and baked for 40 seconds
under 100°C. The result of trial 1 fails to achieve the
goal of making sugar cubes because it became sticky and
jelly like form. In trial 2, before the juice extraction
of ripe Aratiles fruit, it weighs 87.5g. Then, the
extracted juice was 0.0875L and spread into the baking
paper and baked for 90 seconds under 100°C. The result
of trial 2 fails to achieve the goal of making sugar
cubes because the sweetener got burned. In trial 3, the
researchers decided to put it in a molder and freeze
it. Before the juice extraction of ripe Aratiles fruit,
it weighs 750g. Then, the extracted juice was 0.235L,
it was boiled under 70C for 600 seconds, added 5.69g of
baking powder, and the juice reached 100C. The boiled
extract was cooled down at room temperature before
putting in the molder. Trial 3 formed 160 cubes (1ml
each) and stored under freezer with a temperature of
2.4°C. After 12 hours, the sugar cubes have temperature
of 6.7°C. Thus, trial 3 was successful for achieving
the goal of making sugar cubes under cool temperature.
45
TABLE 1.2: The Measurements of Treatment 2 – Ripe and
Unripe Aratiles Sugar
Treatment 1 Ripe Aratiles Trial 1 Trial 2 Trial 3
Sugar
Mass of Aratiles (g) 250g 87.5g 750g
Temperature (°C) 100°C 100°C 70°C
(oven) (oven) (stove)
Temperature (C) (Boiling N/A N/A 100°C
point)
Time (s) 190s 60s 600s
Volume (L) 0.25L 0.0875L 0.235L
Number of cubes N/A N/A 160 cubes
Amount of Baking Powder N/A N/A 5.69g
Storage Temperature (C) N/A N/A 2.4°C
Temperature (C) N/A N/A 6.7°C
The table shows the measurements of trial 1, trial
2, and trial 3 of treatment 1 (Combination of ripe and
unripe Aratiles). In trial 1, before the juice
46
extraction of Aratiles fruit, it weighs 250g. Then, the
extracted juice was 0.25L and spread into the baking
paper and baked for 190 seconds under 100°C. The result
of trial 1 fails to achieve the goal of making sugar
cubes because it burned. In trial 2, before the juice
extraction of Aratiles fruit, it weighs 87.5g. Then,
the extracted juice was 0.0875L and spread into the
baking paper and baked for 60 seconds under 100°C. The
result of trial 2 fails to achieve the goal of making
sugar cubes because the sweetener got burned too. In
trial 3, the researchers decided to put it in a molder
and freeze it. Before the juice extraction of ripe
Aratiles fruit, it weighs 750g. Then, the extracted
juice was 0.235L, it was boiled under 70°C for 600
seconds, added 5.69g of baking powder, and the juice
reached 100°C. The boiled extract was cooled down at
room temperature before putting in the molder. Trial 3
formed 160 cubes (1ml each) and stored under freezer
with a temperature of 2.4°C. After 12 hours, the sugar
cubes have temperature of 6.7°C. Thus, trial 3 was
successful for achieving the goal of making sugar cubes
under cool temperature.
47
TABLE 2.1 Level of evaluation of T1-ripe Aratiles sugar
and T2- combination of ripe and unripe Aratiles Sugar
in terms of taste.
Treatment Mean Verbal Rank
Interpretation
T1 – Ripe Aratiles Sugar 3.60 Like Slightly 1
T2 – Combination of Ripe and 3.59 Like Slightly 2
Unripe Aratiles Sugar
General Weighted Mean (GWM) 3.60 Like Slightly
Taste of Ripe and Combination Ripe and Unripe Aratiles
Sugar
As shown in Table 2.1, the ripe Aratiles sugar
obtained the highest rank and gained weighted mean of
3.60. It has sweet taste that was like slightly by the
respondents. On the other hand, the ripe and unripe
Aratiles sugar gained a weighted mean of 3.59 which
indicates liked slightly by the respondents. Therefore,
we conclude that Aratiles sweetener is liked slightly
by the respondents. With that, according to the studies,
consumers have interest in natural sweeteners, however,
many of them disagree on taste because the food
48
sweetened must have desirable flavor ( Cernivec, 2014;
Li et al., 2014; Oltman et al., 2015).
TABLE 2.2 Level of evaluation of T1-ripe Aratiles sugar
and T2-ripe and unripe Aratiles Sugar in terms of aroma.
Treatment Mean Verbal Rank
Interpretation
T1 – Ripe Aratiles Sugar 4.22 Like 1
Moderately
T2 – Combination of Ripe and 4.05 Like 2
Unripe Aratiles Sugar Moderately
General Weighted Mean (GWM) 4.14 Like Moderately
Aroma of Ripe and Combination of Ripe and Unripe
Aratiles Sugar
As seen on Table 2.2, the evaluation of the
alternative sweetener in terms of its aroma, the
treatment 1 which is the ripe Aratiles got the highest
mean of 4.22 that indicates like moderately. It was
followed by treatment 2 which is the combination of ripe
and unripe Aratiles got a 4.05 mean indicates the like
moderately. These indicate that they prefer the aroma
of treatment 1, which is the pure ripe Aratiles over
49
the aroma of treatment 2. In relation to that, aroma
compounds will most likely shift in some way. The color
and look are the first properties that are perceived
and credited to attract customers to a product, but the
aroma has the greatest impact on the desire to consume
and accept a specific product. (Barrett, et al., 2010)
TABLE 2.3 Level of evaluation of T1-ripe Aratiles sugar
and T2-ripe and unripe Aratiles Sugar in terms of
texture.
Treatment Mean Verbal Rank
Interpretation
T1 – Ripe Aratiles Sugar 3.46 Like Slightly 2
T2 – Combination of Ripe and 3.57 Like 1
Unripe Aratiles Sugar Moderately
General Weighted Mean (GWM) 3.52 Like Moderately
Texture of Ripe and Combination of Ripe and Unripe
Aratiles Sugar
Table 2.3 shows the evaluation of the alternative
sweetener in terms of its texture. Treatment 2 which
is a combination of ripe and unripe Aratiles got the
highest mean of 3.57 indicates like moderately. On the
50
other hand, treatment 1 which is the ripe Aratiles got
a mean 3.46 indicates like slightly. These mean that
the respondents prefer the texture of treatment 2 than
treatment 1. In relation to that, Jeltema et al., (2016)
found that consumers may be classified based on how they
manipulate food in their mouths (Mouth Behavior (MB))
and that these types of people had different food
texture preferences. Moreover, Jeltema and Beckley
proposed the existence of Mouth Behavior groups through
qualitative observational research in which they saw
that individuals differed in how they preferred to
utilize things in their mouths.
51
TABLE 3.1. Level of evaluation of T1-ripe Aratiles
sugar, T2-ripe and unripe Aratiles Sugar, and commercial
sugar in terms of appearance.
Treatment Mean Verbal Rank
Interpretation
T1 – Ripe Aratiles Sugar 3.59 Like 3
Moderately
T2 – Combination of Ripe and 3.81 Like 2
Unripe Aratiles Sugar Moderately
Commercial Sugar 4.35 Like 1
Moderately
Appearance of Ripe Aratiles Sugar, Combination of Ripe
and Unripe Aratiles Sugar, and Commercial Sugar.
Table 3.1 shows the evaluation of the alternative
sweetener in terms of its appearance and the commercial
sugar got the highest mean of 4.35 indicates like
moderately. It was followed by treatment 2 which is a
combination of ripe and unripe Aratiles got a mean of
3.81 which indicates like moderately and the treatment
1 which is the pure ripe Aratiles got a mean of
3.59 which indicates like moderately. Therefore, we
conclude that respondents still prefer the commercial
52
sugar's appearance over the alternative sweetener. With
regard to that, Endrizzi et al., (2015) said consumers
use visual cues to judge the quality of food they are
meant to eat. Additionally, colors are associated with
consumers with particular food categories since birth
and associate these hues, textures, and tastes
throughout their lives. The sight of food also creates
expectations and beliefs about the satiating
capabilities of the item, which increases its
acceptance.
TABLE 3.2. Level of evaluation of T1-ripe Aratiles
sugar, T2-ripe and unripe Aratiles Sugar, and commercial
sugar in terms of preference.
Treatment Mean Verbal Rank
Interpretation
T1 – Ripe Aratiles Sugar 3.89 Like 2
Moderately
T2 – Combination of Ripe and 3.86 Like 3
Unripe Aratiles Sugar Moderately
Commercial Sugar 4.32 Like 1
Moderately
53
Preference level of Respondents of Ripe Aratiles Sugar,
Combination of Ripe and Unripe Aratiles Sugar, and
Commercial Sugar.
As shown in Table 3.2, commercial sugar got the
highest mean of 4.32 which indicates like moderately.
It was followed by treatment 1 which is the pure ripe
Aratiles got a mean of 3.89 which indicates like
moderately and the treatment 2 which is a combination
of ripe and unripe Aratiles got a mean of 3.86 which
indicates like moderately too. Therefore, it means that
they still prefer commercial sugar over the Aratiles
sugar. In relation to that, food sensory characteristics
are regarded as the most important area in which food
manufacturers can successfully differentiate their
products in order to increase their acceptability. This
study comprehensively discusses the impact of aroma,
appearance, taste, and texture on food acceptability.
Knowledge, innovativeness, attitude, belief, and
perception of specific food products are all consumer
attributes that influence food acceptability. Finally,
the 'feel good' element is an important determinant of
food acceptability. (J.W. Maina, 2018).
54
TABLE 4. The Shelf Stability of Aratiles Sugar
DAY Treatment 1 (ripe) Treatment 2 (combination
of ripe and unripe)
1 The sugar cubes The sugar cubes
immediately melted after 2 immediately melted after 2
hours of removing from the hours of removing from the
freezer. freezer.
2 The sugar cubes completely The sugar cubes completely
melted and has ‘sweat- melted and has ‘sweat-
look’. look’.
3 The sugar cubes developed The sugar cubes developed
molds. molds.
4 The sugar cubes are The sugar cubes are
completely covered with completely covered with
molds. molds.
As shown in table 4, it is the daily observation
of the researchers to test the shelf stability of
Aratiles sweetener both treatment 1 and treatment 2.
The frozen sugar cubes were covered with glutinous
powder to avoid them from sticking to one another. It
is stored in an airtight container and placed at room
temperature for about (30C). On day 1, after 2 hours of
55
being out of the freezer, both t1 and t2 sugar cubes
melted. On the second day, the appearance of the sugar
has sweat-look for both treatments. On day 3, there were
molds appeared to the sugar cubes. On day 4, the sugar
cubes of both treatments are completely covered with
molds. Thus, we conclude that the Aratiles sweetener
failed to pass the shelf stability test.
To determine the stability of the raw sugar and
the effects of prolonged storage in different
warehousing conditions or location. To determine it,
samples were drawn initially and quarterly thereafter
and analyzed for Polarization, Moisture, Color, Ash and
Reducing Sugar. As seen from the results, raw sugar
quality parameters after prolonged storage of two years
differ significantly and have means that are not the
same (Sumagaysay, 2019).
56
TABLE 5. The Acceptability of Aratiles Sugar
One-Sample Test
t df Sig. Mean 95% Confidence
(2- Differen Interval of
tail ce the Difference
ed) Lower Upper
Taste 119.00 1 .005 .59500 .5315 .6585
Aroma 13.353 1 .048 1.13500 .0550 2.2150
Texture 9.364 1 .068 .51500 - 1.2138
.1838
The table shows the One-Sample Test for the
Acceptability of Aratiles alternative sweetener (ripe
and combination of ripe and unripe). The Taste has a P-
value of 0.005 which means that there is significant
difference at p<0.05; Aroma has P-value of 0.048 which
means that there is significant difference at p<0.05;
and Texture has a P-value of 0.068 which means there is
no significant difference at p<0.05. The data above
means that Aratiles alternative sweetener is acceptable
57
in terms of taste and aroma. However, it did not pass
the acceptability test on texture.
TABLE 6. Aratiles Alternative Sweetener Compared to
Commercial Sugar.
One-Sample Test
t d Sig. Mean 95% Confidence
f (2- Differe Interval of
tail nce the Difference
ed) Lower Upper
Appearance 12.385 1 .051 4.02500 - 8.1545
.1045
Preference 18.636 1 .034 4.10000 1.304 6.8954
The table shows the One-Sample Test for the
Aratiles alternative sweetener (ripe and combination
of ripe and unripe) compared to commercial sugar. The
appearance has p-value of 0.051 and it is greater than
0.05 which means it fails to reject the null hypothesis.
On the preference, it has p-value of 0.034 and it is
less than 0.05 which means the researchers reject the
58
null hypothesis. Thus, there is no significant
difference between the appearance of Aratiles
alternative sweetener compared to commercial sugar.
However, in terms of preference, there is a significant
difference between the two which means they still prefer
the commercial sugar than Aratiles alternative
sweetener .
59
CHAPTER V
SUMMARY, CONCLUSION, AND RECOMMENDATION
This chapter includes the summary of the findings
that the researchers have analyzed, conclusions, and
the recommendations for future research.
Summary of Findings
Fruits are common source of alternative
sweeteners. The main objective of this study is to
determine the acceptability of alternative sweetener
from Aratiles fruit through the five research questions
listed:
RQ1: What are the sensory evaluation qualities
given to Aratiles sugar – ripe and combination of
ripe and unripe as alternative sweetener in terms
of:
a. Taste
b. Aroma
c. Texture
RQ2: What are the sensory evaluation qualities
given to Aratiles sugar (ripe and combination of
ripe and unripe) and commercial brown sugar in
terms of:
60
d. Appearance
e. Preference
RQ3: Is there a difference between the population
mean of evaluation score in terms of taste, aroma,
and texture of sweetener made from Aratiles fruit
extract (pure ripe, and combination of ripe and
unripe) to the proposed value of population mean
which is 1.0?
RQ4: How are the Aratiles sugar – pure ripe
Aratiles sugar and combination of ripe and unripe
Aratiles sugar in terms of shelf stability?
The researchers found out that the sensory
evaluation in terms of taste under ripe Aratiles sugar
is rated as ‘Like Slightly’ and for combination of ripe
and unripe Aratiles sugar is also rated ‘Like Slightly’.
Therefore, the p-value proved that Aratiles is
acceptable in terms of taste.
In terms of aroma, under ripe Aratiles sugar is
rated as ‘Like Moderately’ and for combination of ripe
and unripe, it is rated ‘Like Slightly’. Therefore, the
p-value proved that it is acceptable in terms of aroma.
In terms of texture, under ripe Aratiles sugar is
rated as ‘Like Slightly’, for combination of ripe and
61
unripe is ‘Like Moderately’. Therefore, the p-value
proved that Aratiles sweetener is not acceptable in
terms of texture.
Furthermore, in terms of appearance, the ripe
Aratiles sweetener got the lowest rating, for
combination of ripe and unripe was rank 2, and the
commercial sugar got the highest mean. Thus, the p-value
proved that there is no significant difference between
the Aratiles sweetener and commercial sugar in terms of
appearance.
On the other hand, in terms of preference, the
Aratiles sugar – combination of ripe and unripe was the
lowest rate, for ripe Aratiles was rank 2 and the
commercial sugar got the highest rate. Thus, there is
significant difference between Aratiles sweetener and
commercial sugar which means they still prefer to use
the commercial sugar than the Aratiles alternative
sweetener.
The researchers also found out that there is no
significant difference between the mean of Aratiles
sweetener and the proposed value mean 1.0 in terms of
taste and aroma. However, there is a significant
difference in terms of texture.
62
Furthermore, based on the observation of the shelf
stability of Aratiles sugar both T1 and T2, the result
turned out that Aratiles sugar cannot last more than 2
hours out of the freezer and placed on a container at
room temperature. The Aratiles developed moist and mold
after 3-4 days in the container. This means that the
alternative sweetener has very short shelf life unlike
the conventional sugars we used.
Conclusion
The developed sugar from Aratiles has given the
sensory evaluation rating of like slightly to like
moderately in terms of taste, aroma, and texture which
means that they like it as alternative sweetener.
Moreover, since the developed sugar from Aratiles at
two treatments was found to have significant difference
on 1.0 (dislike) in terms of taste and aroma, it proves
that it is acceptable as sweetener. However, it did not
pass the acceptability test on texture.
Furthermore, the Aratiles sweetener is compared to
commercial sugar in terms of appearance and preference.
It is found out that they have no significant difference
in terms of appearance. However, in terms of preference
63
to use, there is a significant difference. Therefore,
we conclude that they still prefer to use the commercial
sugar.
In terms of the shelf stability of developed
Aaratiles sweetener, it cannot retain its cube form
longer than 2 hours and cannot have a shelf life longer
than 4 days. The observation revealed that Aratiles
sweetener has short shelf life under normal room
temperature.
Recommendations
On the basis of the aforementioned findings and
conclusions the researchers recommend:
1. As the study limited only on the sensory
evaluation and shelf stability observations and
these are considered as the weaknesses of the study
and with that, it is highly recommended to
1.1 Continuously conduct study on the sugar
profile of Aratiles
1.2 To determine the physiochemical
components of sugar such as nutritional
value, glycemic index, and glycemic load it
must be tested on Bureau of Food and Drugs
Administration (BFAD)
64
2. To determine if there is a significant
difference on taste, aroma, texture, appearance,
and preference it is recommended to conduct a
similar study with a larger sample and with
categories such as age bracket and sex.
3. It is recommended to use more advanced equipment
to produce different kinds of sugar such as the
granulated sugars.
4. It is recommended to add a method such as mixing
the developed sugar into food or drinks to
determine its efficacy.
5. It is recommended to perform more trials to
achieve a characteristic of a sweetener that could
prolong its shelf life.
65
BIBLIOGRAPHY
66
A. PUBLISHED/BOOKS
Bruyère, O., Ahmed, S. H., Atlan, C., J, B., Bortolotti,
M., Canivenc-Lavier, M., Charrière, S., Girardet,
J., Houdart, S., Kalonji, E., Nadaud, P., Rajas,
F., Slama, G., & Margaritis, I. (2015). Review of
the nutritional benefits and risks related to
intense sweeteners. Review of the Nutritional
Benefits and Risks Related to Intense Sweeteners,
73(1). https://doi.org/10.1186/s13690-015-0092-x
C.T. Miranda, R.B. Olinares, R.R. Corales, V.T. Corales,
S.M. Bulda, M.M. Acosta Acta Horticulturae.
(n.d.).
https://www.actahort.org/members/showpdf?booknrar
nr=1213_11
N. J. Suganob, M. G. A. Bautista, R. Concepcion and E.
Dadios, "Coconut Inflorescence Sap Collection for
Future Food and Bioresource Technological
Advancements," 2022 IEEE 14th International
Conference on Humanoid, Nanotechnology,
Information Technology, Communication and Control,
Environment, and Management (HNICEM), Boracay
Island, Philippines, 2022, pp. 1-6, doi:
67
10.1109/HNICEM57413.2022.10109443.
https://ieeexplore.ieee.org/document/10109443
Pereira, G. a. M., Arruda, H. S., De Morais, D. R.,
Eberlin, M. N., & Pastore, G. M. (2018).
Carbohydrates, volatile and phenolic compounds
composition, and antioxidant activity of calabura
(Muntingia calabura L.) fruit. Food Research
International, 108, 264–273.
https://doi.org/10.1016/j.foodres.2018.03.046
Phillips, K. W., Carlsen, M. H., & Blomhoff, R. (2009).
Total Antioxidant Content of
Alternatives to Refined Sugar. Journal of the
American Dietetic Association, 109(1), 64– 71.
https://doi.org/10.1016/j.jada.2008.10.014
Pereira, G.A., Tome, P.H.F., Arruda, H.S., Fragiorge,
E.J., Ribeiro, P.R. (2016) Physicochemical
characterization and antioxidant activity of
calabura fruit (Muntingia calabura L.). REBRAPA –
Brazilian Journal of Food Research 2016 Vol.7 No.2
pp.67-79 ref.26
Sparks, P. B., & Shepherd, R. (1994). Public Perceptions
of the Potential Hazards Associated with Food
Production and Food Consumption: An Empirical
68
Study. Risk Analysis, 14(5), 799–806.
https://doi.org/10.1111/j.1539-
6924.1994.tb00291.x
B. UNPUBLISHED/THESIS
Mahmood, N. D., Nasir, N. a. M., Rofiee, M. S., Tohid,
S. F. M., Ching, S. M., Teh, L. K., Salleh, M. a.
a. M., & Zakaria, Z. A. (2014). Muntingia calabura:
A review of its traditional uses, chemical
properties, and pharmacological observations.
Pharmaceutical Biology, 52(12), 1598–1623.
https://doi.org/10.3109/13880209.2014.908397
Jeltema, M., Beckley, J., & Vahalik, J. (2015). Model
for understanding consumer textural food choice.
Food Science and Nutrition, 3(3), 202–212.
https://doi.org/10.1002/fsn3.205
Niwele, F. (2020, December 28). Nutritional Enzymes
Inhibitory Activities of Fruit Juice and Fruit Pulp
Extract of Muntingia Calbrura L.
https://www.foodandnutritionjournal.org/volume8nu
mber3/nutritional-enzymes-inhibitory-activities-
of-fruit-juice-and-fruit-pulp-extract-of-
muntingia-calabura-l-2/
69
Saraiva, A., Carrascosa, C., Raheem, D., Ramos, F., &
Raposo, A. (2020). Natural Sweeteners: The
Relevance of Food Naturalness for Consumers, Food
Security Aspects, Sustainability and Health
Impacts. International Journal of Environmental
Research and Public Health, 17(17), 6285.
https://doi.org/10.3390/ijerph17176285
Sholihah, L., Rahmania, U.G., Rumiati, Munawaroh, F.,
Handayani, R.D., & Lesmono, A.D. (2021). Analysis
of physics concept in the traditional brown sugar
making process. J. Phys.: Conf. Ser. 1832 012041.
Analysis of physics concept in the traditional
brown sugar making process - IOPscience
Zakaria, Z. A., Mustapha, S., Sulaiman, M. H., Jais, A.
M. M., Somchit, M. N., & Abdullah, F. (2007). The
Antinociceptive Action of Aqueous Extract from
<i>Muntingia calabura</i> Leaves: The Role of
Opioid Receptors. Medical Principles and Practice,
16(2), 130–136. https://doi.org/10.1159/000098366
Arshad, S., Rehman, T., Saif, S., Rajoka, M. S. R.,
Ranjha, M. M. a. N., Hassoun, A., Cropotova, J.,
Trif, M., Younas, A., & Aadil, R. M. (2022).
Replacement of refined sugar by natural
70
sweeteners: focus on potential health benefits.
Heliyon, 8(9), e10711.
https://doi.org/10.1016/j.heliyon.2022.e10711
Pereira, G.A., Henrique, P., Arruda, H.S., Fragiorge.
E.J., Ribeiro. P.R., (2016)
. 10.3895/rebrapa.v7n2.3526
https://periodicos.utfpr.edu.br/rebrapa/article/v
iew/3526
Sumagaysay, J.N., C (2019) . Determination of Stability
of Philippine Raw Sugar in Storage.
determination_of_raw_sugar_stability_in_storage_j
sumagaysay.pdf (philsutech.com)
C. JOURNAL/ARTICLES
Federica.Ferrari. (2023b). 10 most common types of sugar
and their uses. www.finedininglovers.com.
https://www.finedininglovers.com/article/differen
t-types-sugar-how-use
Aratiles / Muntingia calabura Linn / Cherry tree:
Philippine Medicinal Herbs/ Philippine Alternative
Medicine. (n.d.).
http://www.stuartxchange.org/Aratiles.html
71
Abu, J. D. (2002c). DEVELOPMENT OF A SWEETENER FROM
BLACK PLUM (VITEX DONIANA) FRUIT. International
Journal of Food Properties.
https://doi.org/10.1081/jfp-120015598
Endrizzi I, Torri L, Corollaro ML, Demattè ML, Aprea
E., (2015) A conjoint study on apple acceptability:
Sensory characteristics and nutritional
information. Food quality and preference. 2015;
40:39-48
D. INTERNET
BSc, K. G. (2023, March 29). Coconut Sugar: A Healthy
Sugar Alternative or a Big, Fat Lie? Healthline.
https://www.healthline.com/nutrition/coconut-
sugar#TOC_TITLE_HDR_2
Going beyond refined sugar: Traditional sweeteners in
the Philippines. (2022, July 16). Manila Bulletin.
https://mb.com.ph/2022/07/16/going-beyond-
refined-sugar-traditional-sweeteners-in-the-
philippines/
National Tropical Botanical Garden | Muntingia
calabura - Plant Detail - Tropical Plants
Database. (n.d.). National Tropical Botanical
72
Garden.
https://ntbg.org/database/plants/detail/Muntingia
-calabura
Sweet Stuff. (2017, September 8). NIH News in Health.
https://newsinhealth.nih.gov/2014/10/sweet-stufff
InWEnt/GFU. 2003. Proceedings of the International
Workshop on Underutilized Plant Species. Leipzig,
6-8 May 2003. Retrieved 9 February 2013 from
http:// www. agriculturesnetwork.org
Sugar - Sidney Mintz. (n.d.).
https://sidneymintz.net/sugar.php
Morton, J. F. Fruits of warm climates 1987 pp.517 pp.
ref.p. 446-483
https://www.cabdirect.org/cabdirect/abstract/1987
6763104
73
APPENDICES
74
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
May 11, 2023
MARIESU M. ANTONIO, Ph.D.
Principal
School of Our Lady of Atocha, Inc.
Alicia, Isabela
Dear Ma’am,
Warmest greetings!
In partial fulfillment of the requirements in our Inquiries, Investigation, and Immersion,
we, STEM 12- St. Michael students of School of Our Lady of Atocha, Inc. would like to
conduct a research study entitled, “Muntingia calabura (Aratiles): as an Alternative
Sweetener”.
We are writing this request letter to seek for your permission to conduct a survey to the
Faculty and Staffs of your institution as well as the parents and guardians who go to school
regularly. By doing so, we believe that this will help us obtain the information needed for
our study.
We are hoping and will always be grateful for your positive response.
Respectfully yours,
Althea Faith J. Co
James Arvy B. Dupale
Sharmaine Anne A. Gaspar
Dj Robin O. Mendoza
Karlo Q. Suguitan
Karylle P. Suguitan
Noted: Approved:
MRS. ANAMARIE GARCIA-CASI MARIESU M. ANTONIO, Ph.D.
Research Adviser Principal
75
76
DOCUMENTATION
77
TRIAL EXPERIMENT 1
(T1 &T2)
Harvesting Extracting
Baking Result
78
TRIAL EXPERIMENT 2
(T1 &T2)
Harvesting Extracting
Baking Result
79
TRIAL EXPERIMENT 3
(T1 &T2)
Harvesting Extracting
Boiling Adding baking powder
se
Molding and Freezing Result
80
SHELF STABILITY
(T1 &T2)
T1
T2
81
DATA GATHERING
Survey-Questionnaire Evaluation
Preparation of tools for evaluation.
Evaluation on respondents: faculty, staffs, and
parents.
82
CURRICULUM VITAE
83
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
ALTHEA FAITH J. CO
Loria, Angadanan, Isabela
altheajco01@gmail.com
0955-639-8907
PERSONAL PROFILE
Date of Birth : January 27, 2005
Place of Birth : Loria, Angadanan, Isabela
Citizenship : Filipino
Sex : Female
Age : 18
Civil Status : Single
Religion : Catholic
Father’s Name : Carlos C. Co
Mother’s Name : Josie J. Co
EDUCATIONAL ATTAINMENT
Senior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2021-2023
Junior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2017-2021
Elementary: Angadanan East Central School
Loria, Angadanan, Isabela
2011-2017
84
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
JAMES ARVY B. DUPALE
Sto. Tomas, Alicia, Isabela
jamesarvyd@gmail.com
0995-181-2959
PERSONAL PROFILE
Date of Birth : October 16, 2004
Place of Birth : Paguila Hospital, Alicia, Isabela
Citizenship : Filipino
Sex : Male
Age : 18
Civil Status : Single
Religion : Catholic
Father’s Name : Benjamin F. Dupale
Mother’s Name : Annabelle Barrientos
EDUCATIONAL ATTAINMENT
Senior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2021-2023
Junior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2017-2021
Elementary: Alicia South Central School
Magsaysay, Alicia, Isabela
2011-2017
85
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
SHARMAINE ANN A. GASPAR
Aniog, Angadanan, Isabela
gasparsharmaineann@gmail.com
0966-908-8750
PERSONAL PROFILE
Date of Birth : September 23, 2005
Place of Birth : Vigan City, Ilocos Sur
Citizenship : Filipino
Sex : Female
Age : 17
Civil Status : Single
Religion : Catholic
Father’s Name : Ronald G. Gaspar
Mother’s Name : Annie A. Gaspar
EDUCATIONAL ATTAINMENT
Senior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2021-2023
Junior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2017-2021
Elementary: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2016-2017
86
Angadanan East Central School
Loria, Angadanan, Isabela
2015-2016
Aniog Primary School
Aniog, Angadanan, Isabela
2011-2015
87
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
DJ ROBIN O. MENDOZA
Linglingay, Alicia, Isabela
djrobinmendoza@gmail.com
0977-255-6120
PERSONAL PROFILE
Date of Birth : March 09, 2005
Place of Birth : Linglingay, Alicia, Isabela
Citizenship : Filipino
Sex : Male
Age : 18
Civil Status : Single
Religion : Iglesia ni Cristo
Father’s Name : Hospicio M. Mendoza
Mother’s Name : Mercedita O. Mendoza
EDUCATIONAL ATTAINMENT
Senior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2021-2023
Junior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2017-2021
Elementary: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2011-2017
88
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
KARLO Q. SUGUITAN
Linglingay, Alicia, Isabela
milosuguitan00@gmail.com
0965-128-6991
PERSONAL PROFILE
Date of Birth : May 23, 2005
Place of Birth : Cauayan Hospital
Citizenship : Filipino
Sex : Male
Age : 17
Civil Status : Single
Religion : Born Again/ Christian
Father’s Name : Joel D. Suguitan
Mother’s Name : Adelyn Q. Suguitan
EDUCATIONAL ATTAINMENT
Senior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2022-2023
Junior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2017-2021
Elementary: Alicia South Central School
2011-2014
Alicia East Central School
2014-2017
89
Republic of the Philippines
School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
KARYLLE P. SUGUITAN
Calaocan, Alicia, Isabela
karyllesuguitan258@gmail.com
0936-403-3722
PERSONAL PROFILE
Date of Birth : April 21, 2005
Place of Birth : Munsayac, Alicia, Isabela
Citizenship : Filipino
Sex : Female
Age : 18
Civil Status : Single
Religion : Born Again/ Christian
Father’s Name : Arnold D. Suguitan
Mother’s Name : Rowena P. Suguitan
EDUCATIONAL ATTAINMENT
Senior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2021-2023
Junior High School: School of Our Lady of Atocha, Inc.
Magsaysay, Alicia, Isabela
2017-2021
Elementary: Alicia South Central School
Magsaysay, Alicia, Isabela
2011-2017
90