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Received September 04, 2019; Revised October 14, 2019; Accepted October 25, 2019
Abstract In the present study, the influence of ultrasound (US) and blanching (BL) compared to untreatment (UN)
on the drying rate, color, rehydration, total phenolic content, antioxidant activities and sensory evaluation of cashew
apple were investigated. Ultrasound dehydration of cashew apple followed by BL, respectively, showed the faster
drying time compared to UN. Ultrasound treatment was effective in retaining on whiteness of dried cashew apple.
The color difference of US followed by BL was significantly lower (p<0.05) compared to UN samples. Ultrasound
caused a higher retention of total phenolic and flavonoids content and presented the highest antioxidant power. It
emerged from this study that ultrasound can be employed successfully as pretretment prior to dried cashew apple
with better nutritional attributes.
Keywords: ultrasound, blanching, drying, cashew apple, bioactive compounds
Cite This Article: Camel Lagnika, Abdou Madjid O. Amoussa, Ambaliou Sanni, and Latifou Lagnika,
“Effect of Blanching and Ultrasound on Drying Time, Physicochemical and Bioactive Compounds of
Dried Cashew Apple.” American Journal of Food Science and Technology, vol. 7, no. 6 (2019): 227-233.
doi: 10.12691/ajfst-7-6-10.
[19]. However, a major limitation in the production of cashew apples without any treatment was used as control.
cashew apple powder is the high capital investment in the b) Blanched (BL) samples were boiling in water for 1 min
drying unit operation. Loss of vitamin C due to drying has at 90°C and immediately cooled in distilled water to avoid
been reported in the range of 34-44% [20]. One of the over-processing. c) Ultrasound (US) samples treatments
main purposes of modern food technology is to maximize were carried out using Bioblock Scientific, Vibra-cell
the retention of nutrients during processing and storage. 75115 (with probe diameter of 10 mm) at constant power
Dried foods can be stored for long periods without of 500 W and frequency of 20 kHz for 10 min. The ratio
deterioration because the microorganisms which cause between cashew apples and the liquid medium was
food spoilage and decay are unable to grow and maintained at 1:4 (w/w) [22] for ultrasound pretreatments.
multiply due to insufficient water contents. Unfortunately, After ultrasound processing, the samples were air-dried
convectional drying methods due to their high in a forced circulating air-drying oven (Digital display
temperatures and long drying times lead to high energy BOV-V45F; Zhangqiu, Jinan, Shandong, China) at 60°C.
consumption even quality destruction of final products. During the air-drying, the weights of different samples
Pre-treatments can improve the drying process leading to were measured every 30 min using an automatic digital
high-quality products [21]. Pre-treatment can be used to balance.
reduce the initial water content of the fruit or can be used
to modify the fruit tissue structure in a way that air-drying 2.3. Physico-chemical Analysis
becomes faster [22].
Blanching pre-treatment could reduce the drying time 2.3.1. Color
and improve product quality, and it is an important step
Surface color of fresh and dried cashew apple was
during the processing of fruits and vegetables before
determined by a chroma-meter (CR-410, Konica Minolta
commercial drying [23,24]. It is mainly used to inactivate
Sensing, Tokyo, Japan) with CIE color parameters L*
enzymes, but it is also used to remove air from the
(light/dark), a* (red/green) and b*(yellow/blue). The mean
intercellular spaces in fruits and vegetables [25,26].
values of ten pieces for each sample are used for the
Having been recognized as the effective alternative,
analysis. For the color homogeneity of the dried cashew
ultrasound-assisted drying is applied either as a
apple, the latter had been crushed into powder. Color
pretreatment in liquids to cause lasting changes in the
changes (∆E) were observed in 3 replicates using a
product that improve subsequent drying, or as a
previously described equation.
combination treatment during a drying process to affect
the mass transfer directly. Nowadays application of 1
ultrasound as pre-treatment of fruits prior to drying has ∆E= [( L*t − L*i )2 + (at* − ai* )2 + (bt* − bi* ) 2 ] 2
drawn the attention of researchers to improve drying where: ∆E indicates the degree of overall color change in
rate as well as quality of dried fruits [21,22,27]. This comparison to color values of fresh cashew apple. Li*, ai*
application is said to be relatively new and has not yet and bi* represents those without any treatments and Lt*,
been fully explored as to how ultrasound affects factors in at* and bt* refers to the individual readings of drying
different fruits and it`s cost. samples.
The objective of this study was to investigate the effects
of ultrasound and blanching as a pre-treatment on drying 2.3.2. Rehydration Ratio
kinetics, physicochemical and bioactive compound of Rehydration experiments were performed by immersing
dried cashew apple. approximately 5 g of dried cashew apple into 100 mL of
distilled water at 25°C for 60 min. The samples were then
withdrawn, drained, gently blotted with paper towels to
2. Materials and Methods eliminate the surface water and then weighed. The
rehydration ratio (RR) of the sample was calculated using
2.1. Sample Preparation equation.
The raw material was red cashew apples (Anacardium Weight after rehydratation ( g )
RR = .
occidentale L.) harvested at commercial maturity stage in Weight before rehydratation ( g )
Ketou city, republic of Benin. After the fruit sanitization
and nut removal, the cashew apple (peduncle) were
2.3.3. Total Phenolic Content
reserved. Samples were cut into 10 mm thick slices. The
moisture content of the different samples was determined The samples (2 g) were mixed with 20 mL methanol
by the AOAC Official Method. About 5 g of sample was and sonicated for 30 minutes. The homogenate was
placed in an aluminium disk and dried to a constant centrifuged at 10000 rpm for 10 min at 4°C. Its
weight at 105°C in hot air-drying oven for 24h. The initial supernatant was filtered through Whatman No.1 filter
moisture content was 87.20 ± 0.72 g/100 g (wet basis, paper and 1 mL of the filtrate was diluted to 10 mL with
w.b.). Total soluble solid was 10 °Brix and determined methanol and analyzed for total phenolic content using
using a handheld refractometer (Hanna Instruments, Italy). Folin-Ciocalteau method with slight modifications described
by Aberoumand et al. [28] Briefly, 200 μL of the extract
solution was mixed with 1 mL of Folin-Ciocalteu phenol
2.2. Experimental Design
reagent for 10 min using a Vortex mixer. Next, 800 µL of
The fresh slices cashew apples were divided into three sodium carbonate (7.5%) was added. The solution was
groups: a) Untreated (UN) groups is the fresh slices vortexed and allowed to stand for 2 h at room temperature.
American Journal of Food Science and Technology 229
The absorbance was measured at 765 nm with a panelist. The aspects were evaluated on a scale of 9-1
spectrophotometer (UV-1600PC. Shanghai. China). The points, where 9 = liked extremely, 8 = liked very much, 7
calibration curve of gallic acid was made and the total = liked moderately, 6 = liked slightly, 5 = liked (limit of
phenolic content was calculated and expressed as mg acceptable), 4 = disliked slightly, 3 = disliked moderately,
gallic acid equivalent (GAE)/100 mg for the samples. 2 = disliked much and 1 = disliked extremely.
2.3.4. Flavonoids
2.5. Statistical Analysis
Flavonoids content were determined according to the
methods used by Amoussa et al. (2015) [29]. Namely, All experiments were performed in triplicate. Data
0.5 mL of extract (1mgmL−1) was added to 1.5 mL of were expressed as mean ± standard deviation (SD). The
methanol, 0.1 mL of potassium acetate (1M) and 0.1 mL Tukey’s test and one-way analysis of variance (ANOVA)
of trichloride aluminium (AlCl3) in methanol (10%), to all used for multiple comparisons by the SPSS 17.0 (SPSS,
is added 2.8 mL of distilled water. The absorbance was Chicago, USA). Difference was considered to be
read at 415 nm after 30 minutes of incubation against a statistically significant if P < 0.05.
blank. Thus, obtaining a stable yellow color allows to
evaluate by spectrophotometer (UV-1600PC, Shanghai,
China). The results were expressed in mg / ml equivalents 3. Results and Discussions
quercetin (mg / ml).
3.1. Effect of Pretreatments on Drying
2.3.5. DPPH Assay Scavenging Activity Process of Cashew Apple Slices
The DPPH (1.1-diphenyl-2-picrylhydrazy) radical
scavenging activity of the flesh and dried samples was The effects of different pretreatments on the drying rate
determined according to Turkmen et al. [30] The samples of cashew apple slices dried by hot air-drying were shown
(0.5 g) were mixed in 50 mL methanol, then filtered with in Figure 1. The moisture content decreased continuously
a filter paper No. 4. Then, 1 mL of the extract solution with the extension of drying time. The moisture content of
was mixed and vigorously shaken with 2 mL of 0.1 mM the samples decreased with the increasing HAD time. At
DPPH solution in methanol. The mixture was left to stand the initial stages of drying (t < 2 h), the drying curves for
for 60 min in the dark. The absorbance was measured at UN and BL samples did not deviate much from each other.
517 nm. The percentage of DPPH scavenging activity was However, after this time, the BL sample began to show a
calculated as [1-(absorbance of sample/absorbance of steady decrease in drying rate, whereas the US samples
control)] × 100. maintained greater drying rates. The results indicated that
the different pretreatments played an important role in
2.3.6. Vitamin C content drying time. Ultrasound dehydration of cashew apple
followed by BL, respectively, showed the faster drying
Vitamin C content in the fresh and MVD sample was
time compared to UN. Thus, for example, after 4 h of
determined using High-Performance Liquid Chromatography
drying at 60°C, the moisture content of different samples
(HPLC) according to Spinola et al. [31] The samples
reached 12.55, 16.34 and 20.48 for US, BL, and UN,
(2.5 g) were added to 25 mL of extraction solution
respectively. Ultrasound treatments were relatively more
(3% MPA), vortexed in the darkness, and then centrifuged
effective regarding drying time than other treatments. This
for 10 min with a velocity of 6000 rpm. The supernatant
could be due to the ultrasonic energy that penetrates into
was filtered through 0.22 µm PTFE filters (Milipore.
the samples during the compressions and expansions and
USA). All operations were performed under reduced light.
causes the “perturbation” effect which could not only
An isocratic mobile phase consisting of aqueous 0.2% (v/v)
expand capillary channels for moisture diffusion and
MPA with a flow rate of 0.8 mL/min was used. The
produce microcapillary channels, but also help water
volume of injection was 20 µL and the detection
molecules inside the samples to overcome the binding
wavelength for the PDA was equalled at 240 nm. The
force from organizational structure which might have
L-ascorbic acid was determined by a comparison with
eased moisture removal and increased the diffusivity
the standardized retention time matching with the UV
of the water [22,32]. This result confirms the observations
absorption spectrum. The analysis was carried out in
of De la Fuente-Blanco et al. [33] who showed that
duplicate for each type of sample.
ultrasonic pretreatment affects the fruit tissue making it
easier for the water to diffuse during air-drying and
2.4. Sensory Evaluation of Tea showed that the microscopic channels may contribute with
from Cashew Apple Dried the higher water [22]. It was also shown that the drying
rate was increased by the blanching treatment compared to
For the sensory evaluation, the dried cashew apple had untreated samples. The causes of the increase in drying
been crushed into powder to make tea. The tea option was rate of the blanched samples are probably due to the
chosen because of its high bioactive content. Sensory increasing penetration of water into the sample surface by
evaluation of the cashew apple tea was performed with a decreasing the hardening of the sample surface [34], or by
25 panel at Laboratory of Biochemistry and Bioactive the destroying of cell membrane stability [35], and
Natural Substances. The quality attributes tested were changing the resistance to internal moisture diffusion by
color, aroma, taste and overall quality. Samples were altering the microstructure due to physical damage to the
randomly coded with three-digit numbers and their order sample [36]. These factors interact to enhance the drying
of presentation was completely randomized for each rate.
230 American Journal of Food Science and Technology
Figure 1. Evolution of the moisture content during drying time at 60°C of different treatments of cashew apple samples
Table 1. Color parameters of dried cashew apple after different pretreatment conditions
Color
Rehydration ratio
L* a* b* ∆E
FR 70.79±0.93c 6.45±1.31a 40.24±0.87c -
a b
UN 51.23±0.33 10.51±1.04 9.08±1.11a 37.01±0.21c 1.94
b b b b
BL 59.28±0.66 11.33±1.37 16.13±0.59 27.15±0.77 2.14
US 68.25±0.16c 11.92±0.87b 18.63±0.33b 22.43±0.96a 2.24
Values are Mean±standard deviation (n = 3). Data in same column with different letters are significantly different (p<0.05).
3.2. Color (p < 0.05) were observed in color of the dried samples
in comparison to fresh samples. The L*, a*, b* mean
Color is an important quality attribute that affects the values for fresh cashew apple were 70.79, 6.45 and 40.24,
appearance, presentation, and acceptability of many foods. respectively. In comparison of FR sample, the dried
Color parameters L*, a*, b* and ΔE changes of cashew cashew apple showed a decrease in chromatic parameters
apple dried were shown in Table 1. Significant differences L* (lightness) for UN and BL sample while no significant
American Journal of Food Science and Technology 231
difference with US treatment. On the other hand, there is a to pores in vegetal tissue and consequently improve the
decrease in the value of b* (yellowness) and an increase of extraction of polyphenols [27]. However, blanching
a* (redness). As reported in the literature in the case of caused a significant reduction in the total phenolic content.
dehydrated apples and carrots, the pre-treatment with Most of the researchers attributed the decrease of TPC to
ultrasound leads to an increase in lightness (L*) compared loss of water soluble by leaching or the thermal
to the untreated fruit [37] which could be connected to US degradation of phenolic compounds during blanching
treatment in liquid medium. Ultrasound treatment was [44,45,46].
effective in retaining on whiteness of dried cashew apple Similar to total phenolic, flavonoids presented also the
(Figure 2). When the plant tissue is immersed in the same profiles (Table 2). In this study, like with total
medium during the US treatment, the color is better phenolics, ultrasound caused a higher retention of
preserved due to the limited access of air [38]. The higher flavonoids. Similar results have been reported for the
ΔE value is related to the bigger color difference between studies of carrot-grape juice [47].
the initial samples and the pretreated samples. The color
difference of US followed by BL was significantly lower 3.5. DPPH Assay Scavenging Activity
(p<0.05) compared to UN samples. Thus, the higher ΔE of
UN and BL pretreatment was probably due to the longer DPPH results of different samples used in this study are
drying time. also included in Table 2. Ultrasound had a higher level of
reduction in DPPH in contrast with BL and UN one. There
3.3. Rehydration Ratio was no significant difference (P > 0.05) between BL and
UN samples. However, a high antioxidant activity of US
Rehydration is a complex process aims at the restoration sample might be due to that technique increased bound
of raw material properties and is widely used as a quality antioxidants such as phenolics and ascorbic acid contents,
indicator. Rehydration indicates the chemical and leading to increased antioxidant activity. Further, ultrasonic
physical changes caused by drying and pretreatments [39]. treatment may inactivate enzymes, for instance, polyphenol
Table 1 displays the effect of different pretreatments on oxidases which are responsible for enzymatic browning
rehydration of the dried cashew apple. US followed by BL leading to improved DPPH values [47].
samples exhibited the higher rehydration ratio as compared
to UN samples. This can be explained by the formation of 3.6. Vitamin C
micro-channels by ultrasound pretreatment, which turned
into pores on the surface of dried cashew apple that The effects of ultrasound and blanching on vitamin C of
enhances the mass transfer through the pores of the cashew apple compared to untreatment sample are shown
sample. The water penetrated into the pores of the samples on Table 2. The vitamin C content of cashew apple treated
more easily during the rehydration process. Similar results whit US was significantly higher than that of BL and UN.
were also found for carrot [40,41] and in apple samples Such result might be related to a disruption of cell wall as
[42]. a result of alternate compression and expansion force on
the solid sample which loosened the bound vitamin C
3.4. Total Phenolic Content (TPC) [48]. It was also discovered that the vitamin C content
of blanching sample was similar to fresh (untreated)
and Total Flavonoids (TF) sample.
Polyphenolic compounds are very important fruit
constituents because of their antioxidant activity in 3.7. Sensory Evaluation of Tea from Dried
chelating redox-active metal ions, inactivating lipid free Cashew Apple
radical chains and preventing hydroperoxide conversion
into reactive oxyradicals. Total phenol contents of cashew Figure 3 shows the sensory evaluation including color,
apple from different treatments are presented in aroma, taste and overall preference of different treatments.
Table 2. TPC value was high in US (2.41±0.69) followed It was shown that US treatment presented the higher score
by UN (1.80±0.88) and BL (1.05±0.37) pretreatments, for color, taste and overall quality. Blanching treatment
respectively. Data obtained from this study for total was affected the sensory attribution in terms of taste but
phenolics in UN samples were comparable with that of had higher scores in the aroma than the other treatments.
Adou et al., (2012) [43]. In this study, the higher TPC Based on the overall quality, US following by BL samples
in US samples may be related to an increased reached the higher score than UN treatment. In conclusion,
extractability for some of the antioxidant components ultrasound improved the sensory quality of cashew apple
following ultrasound processing, which can give rise tea.
Table 2. Total phenolics, total flavonoids, DPPH free-radical scavenging activity and vitamin C of different samples of dried cashew apple
Samples Total phenolic (mg/ml) Total flavonoids (mg/ml) DPPH (%) Vitamin C (mg/100g)
a a a
UN 1.80±0.88 0.12±0.88 74.46 3.01±0.13b
BL 1.05±0.37a 0.10±0.88a 75.04a 2.9±0.74a
b b b
US 2.41±0.69 0.24±0.88 82.89 5.7±0.28c
Values are Mean±standard deviation (n = 3). Data in same column with different letters are significantly different (p<0.05).
232 American Journal of Food Science and Technology
Figure 3. Sensory panel evaluation of dried cashew apple after different pre-treatments
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