University of Veterinary & Animal Sciences (UVAS), Lahore
uvas.edu.pk/facility/u-d-l/index.htm
University Diagnostic Laboratory
Our mission is to protect and promote animal and human health through early detection and monitoring of animal
diseases.
The University Diagnostic Lab (UDL) of the University of Veterinary & Animal Sciences, (UVAS) Lahore is a national leader in
providing rapid diagnosis of animal diseases, identifying emerging diseases, developing new diagnostic methods and
training diagnosticians, and veterinarians. Recently, UDL has merged with newly formed Institute of Microbiology, UVAS. UDL
serves the interests of the livestock and poultry industry, animal research scientists and educators, and companion animal
owners.
In 2009, UDL became the Pakistan's first ISO 17025 accredited veterinary disease diagnostic lab that provides ISO-certified
diagnostic tests for the identification and monitoring emerging diseases. UDL facultyalsoworks on developing new
diagnostic methods. The lab obtained its accreditation from Pakistan National Accreditation Council (PNAC), which has
mutual recognition arrangements (MRAs) with International Laboratory Accreditation Cooperation (ILAC) and Asia Pacific
Laboratory Accreditation Cooperation (APLAC). It is a milestone in the history of UVAS and livestock sector of Pakistan
that a veterinary disease diagnostic laboratory's results are now acceptable worldwide.The lab has also implemented a
computer based "Diagnostic Information Management System (DIMS)" to ensure the good lab practices (GLP) and
confidentiality of the client results. UDL provides quality diagnostic services for all animal species, including necropsy of
birds, bacteriology, clinical chemistry, serology, virology, parasitology, molecular diagnostics, and toxicology.
Staff Position
COORDINATOR
Prof. Dr. Aamir Ghafoor
PROFESSORs
Prof. Dr. Aamir Ghafoor
Prof. Dr. Arfan Ahmad
ASSOCIATE PROFESSOR
Dr. Fareeha Akhtar
ASSISTANT PROFESSOR
Dr. Muhammad Suleman
Publications:
Complete Genome Sequences of 16 Mycoplasma bovis Isolates from Canadian Bison and Cattle. Karen B Register, Darrell
O Bayles, Hao Ma, M Claire Windeyer, Jose Perez-Casal, Ana L Bras, Muhammad Suleman, Murray Woodbury, Murray D
Jelinski, David P Alt. Microbiol Resour Announc. 2020 Jun 4;9(23):e00325-20. doi: 10.1128/MRA.00325-20.
Effect of storage temperature on the microbiological quality of fish meat from two different managemental
systems. Ridda Haroon, Fareeha Akhtar, Ali Ahmad Sheikh. Accepted in Pakistan journal of zoology. 2020
Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat
Aftab Ahmad, Fareeha Akhtar, Muhammad Ilyas Khan, Hameed Ur Rehman, Syeda Alia Gerdazi, Uzma Ghani, Muhammad
Hanzalah Khan, Uzma Ayaz, Hira Mushtaq, Nausheen Abdul Malik, Nasir Mahmood, Matiullah,Wahid Shah, Malik Nawaz
Shuja. 2019. Int. J. Biosci. 14(4), 305-316,
Study of the current resistance pattern to quinolones and other antimicrobials by multidrug resistant Escherichia coli
recovered from retail chicken meat. F. Yasin, A. A. Sheikh, M. Rabbani, A. Aslam, M. I. Rasheed and R. Tanvir. The Journal of
Animal & Plant Sciences, 29(6): 1580-1586.
Immune modulatory and anti-oxidative effect of selenium against pulmonary tuberculosis. Muhammad Imran Hussain,
Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen, Shahbaz Ali
and Sannaullah Iqbal (2019). Pak. J. Pharm. Sci., Vol.32, No.2(Suppl), 779-784.
Immune boosting role of vitamin E against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed,
Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen and Ashraf Mahmood (2019).
Pak. J. Pharm. Sci., Vol.32, No.1(Suppl), 269-276
Effect of carboxylated poly L-Lysine as a cryoprotectant on post-thaw quality and in vivo fertility of Nili Ravi buffalo
(Bubalus bubalis) bull semen. Tariq, A., Ahmad, M., Iqbal, S., Riaz, M. I., Tahir, M. Z., Ghafoor, A., & Riaz, A. (2019).
Theriogenology.
Saleem N, Nawaz M, Ghafoor A, Javeed A, Mustafa A, Rizwan M. 2018. Phenotypic and Molecular Analysis of Antibiotic
Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan. Pakistan Veterinary Journal. DOI:
10.29261/pakvetj/2018.084.
Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid MF, Yaqub S, Smith G, Su YC, Naeem M. 2018. Prevalence and
Phylogenentics of H9N2 in Backyard and Commercial Poultry in Pakistan. Avian Diseases. 62: 1-9.
Jibreel M, Ahmad A, Rabbani M, Mushtaq H, Ghafoor A, Shabbir MZ, Saleem MH, Saleemi MK, Avais M, Muhammad J,
Rehman H. 2018. Evaluation of Drinking Water Quality at Zoo and Various Public Places in District Lahore, Pakistan.
Journal of Animal and Plant Sciences. 28(5): 1314-1320.
Nasim F, Javeed A, Ashraf M, Ghafoor A. 2018. Evaluation of immunomodulatory activity of tenoxicam in mice. Tropical
Journal of Pharmaceutical Research. 17(9):1811-6.
Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid M, et al. 2019. Avian Influenza A (H9N2) Virus in Poultry Worker,
Pakistan, 2015. Emerg Infect Dis. 25(1):136-139. Doi.org/10.3201/eid2501.180618.
Safdar I, Sheikh AA, Akhtar F, Muhammad J , Hussain T, Gohar M, Kanwal A and Tabassum A. 2018. Optimization of
multiplex PCR for detection of gram positive foodborne pathogens from retail quail meat. Pak. J. Agri. Sci. 55(4), 955-958.
Ahmad A, Akhtar F,Khan IM, Rehman UH, Gerdazi AS, Ghani U, Khan MH, Ayaz U , Mushtaq H, Malik AN , Mahmood N,
Matiullah , Shah W, Shuja NM. 2019. Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish,
chicken and beef meat. International Journal of Biosciences. 14(4): 305-316.
Tanvir R, Sheikh AA, Javeed A. 2018. Endophytic Actinomycetes in the Biosynthesis of Bioactive Metabolites: Chemical
Diversity and the Role of Medicinal Plants. In Studies in Natural Products Chemistry. Elsevier. 60: 399-424.
Nawaz F, Tanvir R, Nawaz M, Javeed A, Sajid I. 2018. The effects of polar and non polar compounds from endophytic
actinomycetes in Ocmium tenuiflorum (Tulsi) and Azadirachta indica (Neem) on veterinary and human pathogens.
Proceedings of 4th International Electronic Conference on Medicinal Chemistry ECMC-4. Doi:10.3390/ecmc-4-05567
Laboratories Sections
Bacteriology/Mycology
Molecular Biology
Serology
Parasitology
Diagnostic Services
UDL offers a wide range of testing at affordable prices (see list below).
Scope of testing
The latest tests range offered by UDL include:
TEST OFFERED AT UDL Rates
(Rs.)
POULTRY
Postmortem for poultry (For one shed) 300
ELISA for detection of antibodies against Infectious bronchitis 500
ELISA for detection of antibodies against IBD (Gumboro) virus 500
ELISA for detection of Antibodies against Mycoplasma gallisepticum 500
ELISA for detection of Antibodies against Mycoplasma synoviae 500
HI Test for antibodies against New Castle disease (ND) or Avian Influenza (AI) or Egg Drop Syndrome (EDS) 80
viruses, (per sample)
Culture and sensitivity Test 1000
Total viable count (water) 1200
Total viable count (feed, feed ingredients, Hatchery fluff, etc.) 2000
Coliform count (water) 1500
Coliform count (feed, feed ingredients, Hatchery fluff, etc.) 2000
Isolation and identification of Salmonella (Species) 3000
Isolation and identification of Cryonic 3000
Isolation and identification of E. coli (Species) 2500
Isolation and identification of Staphylococcus (Species) 2500
Isolation and identification of Pasteurella multocida 2500
Isolation and identification of Mycoplasma gallisepticum 3000
Isolation and identification of clostridium spp 5000
Isolation and identification of bacteria from food/ commercial product 3500
Plate exposure for bacteria (Environmental testing) / swab testing (one plate) 2000
Plate exposure for Yeast and Mold Count 3000
Antibacterial activity of disinfectant (single bacteria) 30000
Antiviral activity of disinfectant (single virus) 35000
Isolation and identification of Avian Influenza (AI) & New Castle disease (ND) Virus 5000
PCR for diagnosis of Avian Influenza (H9N2) virus 5000
PCR for diagnosis of Newcastle Disease virus 5000
PCR for diagnosis of Mycoplasma gallisepticum 5000
PCR for diagnosis of Salmonella spp 5000
PCR for diagnosis of Infectious Bronchitis Virus 5000
BOVINES
ELISA for detection of Antibodies against Foot & Mouth Disease Virus (FMDV), (Specific for Cattle & Buffalo) 1000
ELISA for detection of Antibodies against Infection bovine Rhinotracheitis (IBR) 1000
ELISA for detection of Antibodies against Brucella abortus 1000
ELISA for detection of Antibodies against Johne’s disease 1000
ELISA for detection of Antibodies against Bovine Tuberculosis (TB) 1000
ELISA for detection of Bovine viral Diarrhea Virus (BVDV) 1000
ELISA for detection of Bovine leucosis 1000
Rose Bengal Plate test for brucellosis 500
Tuberculin Test (Local antigen) for detection of tuberculosis 500
PCR for diagnosis of Brucella abortus 5000
PCR for diagnosis of Babesia bovis 3000
PCR for diagnosis of Theileria annulata 3000
PCR for diagnosis of Anaplasma spp 3000
PCR for diagnosis of Listeria monocytogenes 5000
PCR for diagnosis of Pasteurella multocida 5000
PCR for diagnosis of Bovine Viral diarrhea virus (BVDV) 5000
PCR for diagnosis of Lumpy Skin Disease Virus (LSDV) 5000
PCR for diagnosis of Mycobacterium tuberculosis 5000
PCR for diagnosis of Mycobacterium bovis 5000
PCR for diagnosis of Campylobacter jejuni 5000
PCR for diagnosis of Bovine ephemeral fever 5000
PCR for diagnosis of Leptospira spp 5000
EQUINES
Mallein test (Local antigen) for detection of Glander 1000
PCR for diagnosis of Burkholderia mallei (Glander) 5000
DOGS AND CATS
HA test for Canine Parvovirus (CPV) per sample 500
Strip test for Canine Parvovirus (CPV) in dog 2000
ELISA for detection of Antibodies against Rabies virus (For export documentation) 15000
MISCELLANEOUS TESTS (Per Sample)
Blood test for parasite (Haemoparasites) 300
Complete blood count (Haematological Analyzer) 3 part machine 700
Complete blood count (Haematological Analyzer) 4 part machine 750
White side / surf test for early detection of mastitis per sample 300
Fecal examination for parasites ova detection 300
Fecal examination for parasites Egg per gram (EPG) 1500
Fecal examination for parasites (Floatation technique) 600
Fecal examination for parasites (Sedimentation technique) 600
Skin scrapping for detection of ectoparasites 300
Skin scrapping for detection of fungus 2000
pH of water 1000
Serum Electrolytes
Total serum / plasma Sodium 300
Total serum / plasma Potassium 300
Total serum / plasma Calcium 300
Total serum / plasma Chloride 300
Total serum / plasma protein 300
Total serum / plasma Phosphorus 300
Liver Function Test (LFT)
ALP 1000
ALT
AST
Total Bilirubin
Renal Function Test
Serum / plasma Creatinine 700
Blood Urea Nitrogen
Cost of additional services i.e. preparation of any product, visit of farm (TA/DA, Transportation charges, etc), etc., will
be charged separately as per requirement.
Lab animals are also available on cost basis.
Scope of ISO 17025 testing
Sr. # Materials/Products Types of Range of Minimum Uncertainty of Standard
tested test/Properties measurement detection Measurement
measured limit
Serum ELISA for detection of 0.25-9.0 0.15 0.05 Kit Method
antibodies against IBD (CPC value) (Synbiotic,
USA 2016)
Serum ELISA for detection of 0.25-9.0 0.15 0.05 Kit Method
antibodies against MG (CPC value) (Synbiotic,
USA 2016)
Serum ELISA for detection of 0.25-9.0 0.15 0.05 Kit Method
antibodies against MS (CPC value) (Synbiotic,
USA 2016)
Serum Haemagglutination 3.0-1024 2.0 0.24 OIE (Manual
Inhibition Test for Avian (HI Titre value) updated
Influenza (AI) H5 2019)
Serum Haemagglutination 3.0-1024 2.0 0.24 OIE (Manual
Inhibition Test for Avian (HI Titre value) updated
Influenza (AI) H7 2019)
Haemagglutination 3.0-1024 2.0 0.24 OIE (Manual
Inhibition Test for Avian (HI Titre value) updated
Influenza (AI) H9 2019)
Serum ELISA for detection of 0.25-9.0 0.15 0.05 Kit Method
antibodies against (CPC value) (Synbiotic,
Rabies USA 2016)
Serum ELISA for Foot & Mouth 0.25-9.0 0.15 0.05 Kit Method
Disease (FMD) (CPC value) (Prionics,
USA 2016)
Serum ELISA for detection of 0.25-9.0 0.15 0.05 Kit Method
antibodies against (CPC value) (IDEXX, USA
Brucella 2016)
Serum Rose Bengal Test (RBT) Qualitative Not Not applicable OIE Manual
test applicable Updated 2019
Water pH of water 3:00-10:00 2:00 0.156 AOAC /2016,
20th Edi,
Method #
973.41
Equine Species Mallein Test Qualitative Not Not applicable OIE Manual
test applicable Updated 2019
Teaching, Research & Development
UDL faculty is also involved in teaching, research & development. A number of research projects have been successfully
completed and few are still in process.
S# Title of Project Source of Amounts of
funding funds (Rs) in
Million
1. Preparation of highly specific ELISA Kit on Recombinant outer membrane HEC, Pakistan 9.544
protein (OMP) of Pasteurella multocida B2 to detect Post-vaccination immune Under TDF
response
2. Preparation of live genetically engineered avirulent vaccinal strains derived from HEC, Pakistan 6.714
local highly pathogenic isolates of P. multocida B:2, using gene deletion method Under NRPU
and evaluation of the vaccine by inoculating in mice and calves
3. Hydrogen peroxide based product CareOx35 effect on the health of dairy cows Dexon 0.9
4. Vaccine Efficacy Testing ICI 1.3
5. Effect of Different antibiotics and steroidal Residues in Meat of Chicken and National
their effect on Humans in Punjab Industrial funds
6. Epidemiology and Characterization of Bacterial Pathogens Associated with National (PARC)
Epizootic Ulcerative Syndrome (EUS) and Motile Aeromonas Septicemia (MAS)
from Pakistani Farmed Fish
7. Molecular Characterization of Bovine Pestivirus isolates from dairy animal of National (HEC) 6.022
Pakistan and their use in diagnostic, biosecurity and biological development to
control disease in Pakistan herds
8. Comparative transcriptome analysis of innate and adaptive immune cells during International 5.5
SARS-CoV-2 infection (Qatar)
Human Resource Development
UDL is playing a vital role in human resource development through organizing workshops, providing trainings, refresher
courses and diploma courses for UVAS staff, students, farmers, lab and field professionals and personnel from sister
institutions of the country.
International Linkages
Sr. University / College Country Nature of Cooperation
No.
1 Division of Lab Animal Medicine, David Geffen School of USA Faculty development in Lab Animal
Medicine, University of California, Los Angeles, CA Facility Management
2 Department of Pathobiology, The University of Connecticut, USA Collaborative Research & Faculty
Connecticut Development
3 Institute of Animal Health, Compton UK - do -
4 Animal Diagnostic Laboratory, PennState University USA - do -
5 Atlantic Veterinary College, University of Prince Edward Island Canada Faculty Development
6 Agriculture & Agri Food Cooperation (AAFC) Canada - do -
7 University of Qatar Qatar Collaborative research
8 Vaccine and Infectious Disease Organization- International Canada Collaborative research
Vaccine Center (VIDO-InterVac)
Clients
K & Ns Poultry Farms, Raiwind, Lahore
Supreme Farms, Lahore-Sheikhupura Road
The Livestock Farm, Rakh Dera Chahl, Lahore
RVFC (Mona depot, Abbotabad, Sahiwal etc.)
The Governor House, Lahore
The Zoo, Lahore
The Livestock Farms of the Punjab
The Clinics of UVAS, Lahore.
The Pets Clinics, Lahore
The Camel Owners, Lahore
The Kennel Club, Lahore
National Feed Pvt. Ltd, Raiwind, Lahore
The Race Course Club, Lahore.
The Rangers Lahore
The Police Line, Lahore
Rana Nisar Stud Farm, Phool Nagar, Pattoki
Munir Poultry Farm, Gujranwala
Idara-e-Kissan
The Crystal Feed Industry, Multan
Noor Stud Farm, Sharif City, Raiwind Road
Other Poultry Farmers of Punjab province
Private Veterinary Clinics
Location
University Diagnostic Lab (UDL), University of Veterinary and Animal Sciences – UVAS, Syed Abdul Qadir Jillani (Out Fall)
Road, 54000 Lahore – Pakistan
Regular Business Hours
Monday – Friday
8: 00a.m. - 4:00 p.m.
Parking
Temporary parking for UDL clients on four wheels is available in front of UDL. For motor bikes, in the parking stand on main
outfall road.
UDL
