Antimicrobial Susceptibility Test Discs

Intended Use specimens should not be employed in this test. Refer procedure, which includes
Antimicrobial susceptibility testing of bacterial and fungal isolates is a common and preparation of inoculum(4, 6).
important techniques in most of clinical laboratories. The results of this tests are Clinical Specimen Collection, Handling and Processing: Follow appropriate
used for selection of most appropriate antimicrobial agent(s) for treatment against techniques for handling specimens as per established guidelines. After use,
the infectious organisms. Antimicrobial susceptibility testing is carried out in large contaminated materials must be sterilized by autoclaving before discarding(4, 6).
scale as a guide to antibiotic therapy. The results of antimicrobial susceptibility Test Procedure: Dip a sterile non-toxic cotton swab on a wooden applicator into
testing should be combined with clinical information and experience when selecting the standardized inoculum (turbidity so adjusted, as to obtain confluent growth on
the most appropriate antibiotic for patient. the Petri plate) and rotate the soaked swab firmly against the upper inside wall of
Background & Summary the tube to express excess fluid. Streak the entire agar surface of the plate with the
swab three times, turning the plate at 60o angle between each streaking. Allow the
Over five to six decades have passed, since the discovery of the “miracle drug”
inoculum to dry for 5 - 15 minutes with lid in place.
referred to as antibiotics or antimicrobials. These drugs are still used for control
and treatment of diseases in people, when microbes are the likely cause. Though an Apply the discs using aseptic technique. Deposit the discs with centres at
exhaustive number of antibiotics are discovered, this line of treatment faces severe least 24 mm apart. For fastidious organisms and for Penicillins and Cephalosporins,
setbacks due to emergence of single or multiple drug resistant microorganisms. the discs should preferably be deposited with centres 30 mm apart.
Hence antimicrobial susceptibility testing is carried out in large scale as a guide Incubate immediately at 35 ± 2oC and examine after 16-18 hours or longer, if
to antibiotic therapy(2,7). Currently these methods are based on dilution or diffusion necessary. For fastidious organisms, incubate at appropriate temperature and time.
procedures. Of these the disc method is best suited to routine use for most purposes For fungal cultures, Incubate immediately at 35 ± 2oC and examine each plate after
as compared to the traditional broth-based techniques(9). 20 - 24 hours of incubation. If plate was satisfactorily streaked the resulting zones
of inhibition will be uniformly circular and there will be a semi-confluent lawn of
Principle growth. Read at 48 hours only when insufficient growth is observed after 24 hours
The Kirby-Bauer test is an assay whereby discs of filter paper are impregnated with incubation.
a single concentration of different antibiotics or any chemicals that will diffuse from Result & Interpretation: Measure the zones showing complete inhibition and record
the disc into the agar. The selected antibiotic discs are placed on the surface of an the diameters of the zones to the nearest millimeter using a calibrated instrument.
agar plate which has already been inoculated with test bacteria. During the incubation Zone scales PW096, of dimensions 370 x 65 mm or PW297, a compact (pocket
period, the antibiotics/chemicals diffuse outward from the discs into the agar. This size), of dimensions 200 x 95 mm can be used to measure sizes of zones in the
will create a concentration gradient in the agar which depends on the solubility range of 10 - 40 mm.
of the chemical and its molecular size. The absence of growth of the organism
Please refer to zone size interpretative chart for list of antibiotics, quality control
around the antibiotic discs indicates that, the respected organism is susceptible to
limits & interpreptative criteria.
that antibiotic and the presence of growth around the antibiotic disc indicates the
organism is resistant to that particular antibiotic. This area of no growth around the Warning & Precautions:
disc is known as a zone of inhibition, which is uniformly circular with a confluent 1. For in vitro diagnostic use and are intended for professional use only. These
lawn of growth in the media. discs are intended only for agar diffusion method and not for broth dilution method.
Applications 2. Follow directions for use. Performance of disc depends on use of proper inoculum
The test is useful for determining the antimicrobial susceptibility of aerobes, and control cultures, recommended test medium & proper storage temperature.
anaerobes, non-fastidious and fastidious organisms. It can be used to study local, 3. Accuracy of the test depends on the disc potency, storage & handling, proper
national and international antibiotic resistance surveillance. Along with single disc, inoculum, functional pretested medium plates (nature of medium and its depth),
for convenience & economy of conducting Antimicrobial Susceptibility Tests HiMedia inoculation technique, incubation temperature and time, etc.
provides Hexa, Octo, Dodeca and Icosa Discs. These series of discs gives the 4. To maintain the potency of discs, store the stock containers of discs in the freezer
privilege to study large number of antibiotics at one time. at -20oC. The discs when required for use within a week, may be kept in refrigerator
(below 8oC) and the remainder should be kept with desiccant and tightly closed
Susceptibility Test Procedure:
container caps in the freezer.
Media Preparation: Prepare plates of Mueller Hinton Agar (M173) for use in the
5. Remove the antimicrobial discs from refrigerator to room temperature 1 - 2 hours
Kirby-Bauer Method for rapidly growing aerobic organisms. For fastidious organisms
before use to avoid moisture condensation. Return unused discs to refrigerator
such as Streptococci, Mueller Hinton Agar (M173) is supplemented with 5%
immediately after applications.
sterile, defibrinated blood. For Haemophilus spp, Haemophilus Test Medium and
for N. gonorrhoeae, GC Agar Base (M434) with 1% defined growth supplement are 6. Once a cartridge is opened it is recommended that it should be used within one
recommended respectively. For Fungal cultures use Mueller Hinton Agar (M173) + 2% week.
Glucose + 0.5 mcg/ml Methylene Blue Dye (GMB Medium). The medium in the 7. While reading results :
plates should be sterile and have a depth of about 4 - 5 mm. a) Ignore swarming of Proteus species if zones of inhibition are clearly defined.
Inoculum Preparation: Use only pure cultures. Confirm by Gram-staining before b) Measure the Sulphonamide zones at the margin of heavy growth since
starting susceptibility test. Transfer 4-5 similar colonies with a wire, needle or loop Sulphonamide may not inhibit organisms for several generations and slight
to 5 ml Tryptone Soya Broth (M011) and incubate at 35-37oC for 2-8 hours until light growth may appear within zones of inhibition.
to moderate turbidity develops. Compare the inoculum turbidity with that of standard c) Subculture, reidentify and retest any large colony growing within a clear zone
0.5 McFarland (R092) (prepared by mixing 0.5 ml of 1.175% barium chloride and of inhibition.
99.5 ml of 0.36N sulfuric acid). Dilute the inoculum or incubate further as necessary 8. Control tests using known cultures should be included each time a sensitivity
to attain comparative turbidity. Alternatively, the inoculum can be standardized by test is performed.
other appropriate optical method (0.08 - 0.13 OD turbid suspension at 620 nm).
9. Antibacterial agents other than those listed in Interpretative Chart are in current
Also direct colony suspension method can be used. Prepare a direct colony use. Susceptibility tests employing these agents should be interpreted on the basis
suspension, from 18-24 hour old non-selective media agar plate in broth or saline. of presence or absence of a definite zone of inhibition and should be considered as
Adjust the turbidity to that of standard 0.5 McFarland. This method is recommended only qualitative until the time interpretative zones have been established.
for testing fastidious organisms like Haemophilus spp., Neisseria spp, streptococci
and for testing staphylococci for potential Methicillin or Oxacillin resistance. Storage & Shelf life
For fungal cultures inoculum is prepared by picking five distinct colonies of On receipt, discs should be stored between -20°C to 8°C or strictly at -20°C as
approximately 1mm from 24 hours old culture grown on Sabouraud Dextrose Agar recommended & for prolonged usage discs should be stored at or below -20°C (refer
(M063) and incubated at 35 ± 2oC. Colonies are suspended in 5ml of sterile 0.85% individual product label for details). Use before expiry date on the label.
Saline.Vortex the resulting suspension and adjust the turbidity to yield 1 x 106 -
Disposal
5 x 106 cells /ml (i.e. 0.5 McFarland standard).
After use, antibiotic discs and material that comes into contact with clinical
Type of Specimen: Pure cultures should be derived from specimens obtained sample must be decontaminated and disposed of in accordance with current
from patients prior to the initiation of antimicrobial therapy. Specimens can be of laboratory techniques(5, 6).
bacterial or fungal isolates derived from blood, urine, faeces, pus, CSF etc. Direct

1
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD035 Amikacin AK 30 mcg
Enterobacterales, P. aeruginosa,
17 15-16 14 19-26 20-26 18-26 – – – – – – – – –
Acientobacter & Staphylococcus spp.
Enterobacterales 18 – 18 19-26 – – – – – – – – – – –
Pseudomonas spp. 15 – 15 – – 20-26 – – – – – – – – –
Staphylococcus spp. 15 – 15 – – – – 18-24 – – – – – – –
Coagulase negative staphylococci 22 – 22 – – – – – – – – – – – –
Acinetobacter spp., 19 – 19 – – – – – – – – – – – –
SD063 Amoxyclav 30 mcg
AMC
(Amoxicillin/Clavulanic acid) (20/10)
Enterobacterales 18 14-17 13 18-24 28-36 – 17-22 – – – – – – – –
Haemophilus influenzae &
20 – 19 – – – – – – 15-23 – – – – –
Haemophilus parainfluenzae
Enterobacterales (IV) 19 – 19 18-24 – – 17-22 – – – – – – – –
Enterobacterales
16 – 16 – – – – – – – – – – – –
(uncomplicated UTI only)
Enterobacterales (Infection originating
50 19-49 19 – – – – – – – – – – – –
from the urinary tract)
Haemophilus influenzae 50 15-49 15 – – – – – – – 17–23 – – – –
Moraxella catarrhalis 19 – 19 – – – – – – – – – – – –
Pasteurella multocida 15 – 15 – – – – – – – – – – – –
Burkholderia pseudomallei 50 22-49 22 – – – – – – – – – – – –
SD002 Ampicillin AMP 10 mcg
Enterobacterales 17 14-16 13 15-22 – – 6 – – – – – – – –
Staphylococcus spp. 29 – 28 – 27-35 – – – – – – – – – –
Enterococcus spp. 17 – 16 – – – – – – – – – – – –
Haemophilus influenzae &
22 19-21 18 – – – – – – 13-21 – – – – –
Haemophilus parainfluenzae
Streptococcus spp. beta
24 – – – – – – – – – – – – 30-36 –
haemolytic group
Enterobacterales (IV & Oral) 14 – 14 15-22 – – – – – – – – – – –
SD002A Ampicillin AMP 2 mcg
Staphylococcus saprophyticus 18 – 18 – – – – 15-21 – – – – – – –
Enterococcus spp. 10 8-9 8 – – – – – 15-21 – – – – – –
Streptococcus pneumoniae 22 16-21 16 – – – – – – – – – – 25-31 –
Streptococcus spp. viridans group 21 15-20 15 – – – – – – – – – – – –
Haemophilus influenzae 18 – 18 – – – – – – – – – – – –
Listeria monocytogens (IV) 16 – 16 – – – – – – 6-12 19-25 – – – –
Pasteurella multocida 17 – 17 – – – – – – – – – – – –
Aerococcus sanguinicola & urinae 26 – 26 – – – – – – – – – – – –
SD112 Ampicillin/Sulbactam A/S 10/10mcg
Enterobacterales, Acientobacter 15 12-14 11 19-24 29-37 – 13-19 – – – – – – – –
Haemophilus influenzae &
20 – 19 – – – – – – 14-22 – – – – –
Haemophilus parainfluenzae
Enterobacterales 14 – 14 19-24 – – 13-19 – – – – – – – –
SD204 Azithromycin AZM 15 mcg
Enterobacterales 13 – 12 – – – – – – – – – – – –
Staphylococcus, S.pneumoniae,
Streptococcus spp. Viridians group &
18 14-17 13 – 21-26 – – – – – – – – 19-25 –
Streptococcus spp. beta
haemolytic group
Haemophilus influenzae &
12 – – – – – – – – 13-21 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 30 – – – – – – – – – – – 30-38 – –
Salmonella Typhi 13 – – – – – – – – – – – – – –
Enterobacterales – – – 14-20 – – – – – – – – – – –
Vibrio spp. 16 – 16 – – – – – – – – – – – –
SD064 Azlocillin AZ 75 mcg – – – – – 24-30 – – – – – – – – –
SD212 Aztreonam AT 30 mcg
Enterobacterales 21 18-20 17 28-36 – – 31-38 – – – – 10-16 – – –
P. aeruginosa 22 16-21 15 – – 23-29 – – – – – – – – –
Haemophilus influenzae &
26 – – – – – – – – 30-38 – – – – –
Haemophilus parainfluenzae
Enterobacterales 26 21-25 21 28-36 – – – – – – – 9-17 – – –
Pseudomonas spp. 50 18-49 18 – – 23-29 – – – – – – – – –
*SD003 Bacitracin B 10 units 8 9-12 13 – 12-22 – – – – – – – – – –
SD004 Carbenicillin CB 100 mcg – – – 23-29 – 18-24 – – – – – – – – –
SD157 Cefaclor CF 30 mcg
Enterobacterales, Staphylococcus spp. 18 15-17 14 23-27 27-31 – – – – – – – – 24-32 –
Haemophilus influenzae &
20 17-19 16 – – – – – – – 25-31 – – – –
Haemophilus parainfluenzae
S. pneumoniae 50 28-49 28 – – – – – – – – – – 25-31 –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
* : Not included in CLSI chart; FDA approved performance standards for Antimicrobial Discs obtained from drug manufacturers.
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
2 For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD116 Cefadroxil CFR 30 mcg
Enterobacterales (Uncomplecated UTI only) 12 – 12 14-20 – – – – – – – – – – –
SD048 Cefalexin CN 30 mcg
Enterobacterales (Uncomplecated UTI only) 14 – 14 15-21 – – – – – – – – – – –
SD200 Cefamandole FAM 30 mcg
Enterobacterales, Staphylococcus spp. 18 15-17 14 26-32 26-34 – – – – – – – – – –
SD047 Cefazolin CZ 30 mcg
Enterobacterales 23 20-22 19 21-27 – – – – – – – – – – –
Enterobacterales (uncomplicated UTIs) 15 – 14 – – – – – – – – – – – –
Staphylococcus spp. 18 15-17 14 – 29-35 – – – – – – – – – –
Enterobacterales (E. coli, Klebsiella
50 20-49 20 21-27 – – – – – – – – – – –
except K. aerogenes)
SD218 Cefdinir CDR 5 mcg
Enterobacterales, Staphylococcus spp. 20 17-19 16 24-28 25-32 – – – – – – – 40-49 26-31 –
Haemophilus influenzae &
20 – – – – – – – – – 24-31 – – – –
Haemophilus parainfluenzae
SD219 Cefepime CPM 30 mcg
Enterobacterales 25 19-24 18 31-37 – 25-31 – – – – – – – – –
P. aeruginosa, Acientobacter 18 15-17 14 – 23-29 – – – – – – – – – –
Haemophilus influenzae &
26 – – – – – – – – 25-31 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 31 – – – – – – – – – – – 37-46 – –
Streptococcus spp. Viridians group 24 22-23 21 – – – – – – – – – – 28-35 –
Streptococcus spp. beta
24 – – – – – – – – – – – – – –
haemolytic group
Enterobacterales 27 24-26 24 – – – – – – – – – – – –
Pseudomonas spp. 50 21-49 21 31-37 – 25-31 – – – – – – – – –
Streptococcus spp. viridans group 25 – 25 – – – – – – – – – – 31-37 –
Haemophilus influenzae 28 – 28 – – – – – – – 30-36 – – – –
Moraxella catarrhalis 20 – 20 – – – – – – – – – – – –
Aeromonas spp. 27 24-26 24 – – – – – – – – – – – –
SD211 Cefixime CFM 5 mcg
Enterobacterales 19 16-18 15 20-26 – – – – – – – – – 16-23 –
Haemophilus influenzae &
21 – – – – – – – – 25-33 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 31 – – – – – – – – – – – 37-45 – –
Enterobacterales (Uncomplecated UTI only) 17 – 17 20-26 – – – – – – – – – – –
Haemophilus influenzae 26 – 26 – – – – – – – 29-35 – – – –
Moraxella catarrhalis 21 – 21 – – – – – – – – – – – –
SD244 Cefmetazole CMZ 30 mcg
Enterobacterales 16 13-15 12 26-32 25-34 – – – – 16-21 – – – – –
Neisseria gonorhoeae 33 28-32 27 – – – – – – – – – 31-36 – –
SD248 Cefonicid CID 30 mcg
Enterobacterales 18 15-17 14 25-29 22-28 – – – – – – – – – –
Haemophilus influenzae &
20 17-19 16 – – – – – – – 30-38 – – – –
Haemophilus parainfluenzae
SD072 Cefoperazone CPZ 75 mcg
Enterobacterales 21 16-20 15 28-34 24-33 23-29 – – – – – – – – –
SD040 Cefotaxime (Cephotaxime) CTX 30 mcg
Enterobacterales 26 23-25 22 29-35 – – – – – – – 17-25 – – –
Acientobacter &
23 15-22 14 – 25-31 18-22 – – – – – – – – –
Staphylococcus spp.
Haemophilus influenzae &
26 – – – – – – – – 31-39 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 34 – – – – – – – – – – – – – –
Neisseria gonorhoeae 31 – – – – – – – – – – – 38-48 – –
Streptococcus spp. Viridians group 28 26-27 25 – – – – – – – – – – 31-39 –
Streptococcus spp.
24 – – – – – – – – – – – – – –
beta haemolytic group
SD295E Cefotaxime (Cephotaxime) CTX 5 mcg
Enterobacterales (indications other than
20 17-19 17 25-31 – – – – – – – 12-18 – – –
meningitis)
Enterobacterales (meningitis) 20 – 20 – – – – – – – – – – – –
Haemophilus influenzae 27 – 27 – – – – – – – 29-37 – – – –
Moraxella catarrhalis 20 17-19 17 – – – – – – – – – – – –
Pasteurella multocida 26 – 26 – – – – – – – – – – – –
Streptococcus spp. viridans group 23 – 23 – – – – – – – – – – 28-34 –
Kingella kingae 27 – 27 – – – – – – – – – – – –
Vibrio spp. 21 – 21 – – – – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement. 3
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD249 Cefotetan CTN 30 mcg
Enterobacterales 16 13-15 12 28-34 17-23 – – – – – – – – – –
Neisseria gonorhoeae 26 20-25 19 – – – – – – – – – 30-36 – –
SD041 Cefoxitin (Cephoxitin) CX 30 mcg
Enterobacterales 18 15-17 14 23-29 – – – – – – – – – – –
For S. aureus & S. lugdunensis 22 – 21 – 23-29 – – – – – – – – – –
For Coagulase-negative Staphylococci
except S. lugdunensis & 25 – 24 – – – – – – – – – – – –
S. pseudintermedius
Neisseria gonorhoeae 28 24-27 23 – – – – – – – – – 33-41 – –
Enterobacterales 19 – 19 23-29 – – – – – – – – – – –
Staphylococcus spp. (S. epidermidis) 25 – 25 – – – – 24-30 – – – – – – –
Staphylococcus spp.
(Coagulase- negative Staphylococci 22 – 22 – – – – – – – – – – – –
other than S. epidermidis)
SD725 Cefpodoxime CPD 10 mcg
Enterobacterales, Staphylococcus spp. 21 18-20 17 23-28 19-25 – – – – – – 9-16 – 28-34 –
Haemophilus influenzae &
21 – – – – – – – – 25-31 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 29 – – – – – – – – – – – 35-43 – –
Enterobacterales (Uncomplecated UTI only) – – – 23-28 – – – – – – – 9–16 – 29-35 –
Haemophilus influenzae 26 – 26 – – – – – – – 30-36 – – – –
SD209 Cefprozil CPR 30 mcg
Enterobacterales,
Haemophilus influenzae & 18 15-17 14 21-27 27-33 – – – – – 20-27 – – 25-32 –
Haemophilus parainfluenzae
SD062 Ceftazidime CAZ 30 mcg
Enterobacterales, B. cepacia 21 18-20 17 25-32 – – – – – – – 10-18 – – –
P.aeruginosa, Acientobacter &
18 15-17 14 – 16-20 22-29 – – – – – – – – –
Staphylococcus spp.
Haemophilus influenzae &
26 – – – – – – – – 27-35 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 31 – – – – – – – – – – – 35-43 – –
SD062A Ceftazidime CAZ 10 mcg
Enterobacterales 22 19-21 19 23-29 – – – – – – – 6-12 – – –
Pseudomonas spp. 50 17-49 17 – – 21-27 – – – – – – – – –
Burkholderia pseudomallei 50 17-49 17 – – – – – – – – – – – –
Vibrio spp. 22 – 22 – – – – – – – – – – – –
SD309 Ceftazidime / Avibactam CZA 30/20 mcg
Enterobacterales, P. aeruginosa 21 – 20 27-35 16-22 25-31 28-35 – – – – 21-27 – – –
SD110 Ceftizoxime CZX 30 mcg
Enterobacterales 25 22-24 21 30-36 27-35 12-17 – – – – – – – 28-34 –
Haemophilus influenzae &
26 – – – – – – – – 29-39 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 38 – – – – – – – – – – – 42-51 – –
SD065 Ceftriaxone CTR 30 mcg
Enterobacterales 23 20-22 19 29-35 – – – – – – – 16-24 – – –
P. aeruginosa, Acientobacter &
21 14-20 13 – 22-28 17-23 – – – – – – – – –
Staphylococcus spp.
Haemophilus influenzae &
26 – – – – – – – – 31-39 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 34 – – – – – – – – – – – 39-51 – –
Neisseria gonorhoeae 35 – – – – – – – – – – – – 30-35 –
Streptococcus spp. Viridians group
27 25-26 24 – – – – – – – – – – – –
Streptococcus spp.
beta haemolytic group 24 – – – – – – – – – – – – – –
Enterobacterales (indications other than
25 22-24 22 29-35 – – – – – – – 16-22 – – –
meningitis)
Enterobacterales (meningitis) 25 – 25 – – – – – – – – – – – –
Streptococcus spp viridans group 27 – 27 – – – – – – – – – – 32-38 –
Haemophilus influenzae 32 – 32 – – – – – – – 34-42 – – – –
Moraxella catarrhalis 24 21-23 21 – – – – – – – – – – – –
Kingella kingae 30 – 30 – – – – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.

4 For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD061 Cefuroxime CXM 30 mcg
Enterobacterales (parenteral) &
18 15-17 14 20-26 27-35 – – – – – – – – – –
Staphylococcus spp.
Enterobacterales (oral) 23 15-22 14 – – – – – – – – – – – –
Haemophilus influenzae &
20 17-19 16 – – – – – – – 28-36 – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 31 26-30 25 – – – – – – – – – 33-41 – –
Enterobacterales (IV) 50 20-49 19 – – – – – – – – – – – –
Enterobacterales (Oral) E.coli, Klebsiella
19 – 19 20-26 – – – – – – – – – – –
spp.,
Streptococcus spp. viridans group (IV) 26 – 26 – – – – – – – – – – 28-34 –
Haemophilus influenzae (IV) 27 25-26 25 – – – – – – – 26-34 – – – –
Haemophilus influenzae (Oral) 50 27-49 27 – – – – – – – – – – – –
Moraxella catarrhalis (IV) 21 18-20 18 – – – – – – – – – – – –
Moraxella catarrhalis (Oral) 50 21-49 21 – – – – – – – – – – – –
Kingella kingae (IV) 29 – 29 – – – – – – – – – – – –
SD050 Cephalothin CEP 30 mcg – – – 15-21 29-37 – – – – – – – – 26-32 –
SD006 Chloramphenicol C 30 mcg
Enterobacterales, Staphylococcus &
18 13-17 12 21-27 19-26 – – – – – – – – – –
Enterococcus spp.
Haemophilus influenzae &
29 26-28 25 – – – – – – 31-40 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 26 20-25 19 – – – – – – – – – – 23-27 –
S. pneumoniae 21 – 20 – – – – – – – – – – – –
Streptococcus spp. Viridians group &
Streptococcus spp. 21 18–20 17 – – – – – – – – – – – –
beta haemolytic group
Enterobacterales 17 – 17 21-27 – – – – – – – – – – –
Staphylococcus spp. 18 – 18 – – – – 20-28 – – – – – – –
Streptococcus group A, B, C & G 19 – 19 – – – – – – – – – – – –
S. pneumoniae 21 – 21 – – – – – – – – – – 24-30 –
Haemophilus influenzae 28 – 28 – – – – – – – 31-37 – – – –
Moraxella catarrhalis 30 – 30 – – – – – – – – – – – –
Burkholderia pseudomallei 50 22-49 22 – – – – – – – – – – – –
SD245 Cinoxacin CIN 100 mcg
Enterobacterales 19 15-18 14 26-32 – – – – – – – – – – –
SD060 Ciprofloxacin CIP 5 mcg
Enterobacterales other than S. Typhi
26 22-25 21 29-38 – – – – – – – – – – –
& extraintestinal Salmonella spp.
Acientobacter, Staphylococcus &
21 16-20 15 – 22-30 – – – – – – – – – –
Enterococcus spp.
P. aeruginosa 25 19-24 18 – – 25-33 – – – – – – – – –
For S. Typhi and extraintestinal
31 21-30 20 – – – – – – – – – – – –
Salmonella spp.
Haemophilus influenzae &
21 – – – – – – – – 34-42 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 35 33-34 32 – – – – – – – – – – – –
Neisseria gonorhoeae 41 28-40 27 – – – – – – – – – 48-58 – –
Enterobacterales 25 22-24 22 29-37 – – – – – – – – – 22-28 –
Staphylococcus spp. 50 21-49 21 – – – – 21-27 – – – – – – –
Coagulase-negative Staphylococci 50 24-49 24 – – – – – – – – – – – –
Pseudomonas spp. 50 26-49 26 – – 25-33 – – – – – – – – –
Enterococcus spp. (Uncomplecated UTI only) 15 – 15 – – – – – 19-25 – – – – – –
Acinetobacter spp. 50 21-49 21 – – – – – – – – – – – –
Haemophilus influenzae, 30 – 30 – – – – – – – 32-40 – – – –
Campylobacter spp. 26 – 26 – – – – – – – – – – – 34-42
Moraxella catarrhalis 31 – 31 – – – – – – – – – – – –
Pasteurella multocida 27 – 27 – – – – – – – – – – – –
Corynebacterium spp. 50 25-49 25 – – – – – – – – – – – –
Aerococcus sanguinicola & urinae
21 – 21 – – – – – – – – – – – –
(Uncomplecated UTI only)
Kingella kingae 28 – 28 – – – – – – – – – – – –
Aeromonas spp. 27 24-26 24 – – – – – – – – – – – –
Bacillus spp. 50 23-49 23 – – – – – – – – – – – –
Vibrio spp. 23 – 23 – – – – – – – – – – – –
SD192 Clarithromycin CLR 15 mcg
Staphylococcus spp. 18 14-17 13 – 26-32 – – – – – – – – – –
Haemophilus influenzae &
13 11-12 10 – – – – – – 11-17 – – – – –
Haemophilus parainfluenzae
S. pneumoniae, Streptococcus spp.
Viridians group, Streptococcus spp. 21 17-20 18 – – – – – – – – – – 25-31 –
beta haemolytic group

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement. 5
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD051 Clindamycin CD 2 mcg
Staphylococcus spp. 21 15-20 14 – 24-30 – – – – – – – – – –
S. pneumoniae, Streptococcus spp.
Viridians group, Streptococcus spp. 19 16-18 15 – – – – – – – – – – 19-25 –
beta haemolytic group
Staphylococcus spp. 22 19-21 19 – – – – 23-29 – – – – – – –
Streptococcus spp viridans group &
19 – 19 – – – – – – – – – – 22-28 –
S. pneumoniae
Streptococcus group A, B, C & G 17 – 17 – – – – – – – – – – – –
Corynebacterium spp. 20 – 20 – – – – – – – – – – – –
Bacillus spp. 17 – 17 – – – – – – – – – – – –
SD297 Colistin Sulphate CS 10 mcg – – – 11-17 – 11-17 – – – – – – – – –
SD010 Co–Trimoxazole 25 mcg
COT
(Trimethoprim/Sulphamethoxazole) (1.25/
Enterobacterales, Acientobacter, 23.75)
B. cepacia, S. maltophila, mcg
Staphylococcus, Haemophilus 16 11-15 10 23-29 24-32 – – – >=20 24-32 – – – – –
influenzae & Haemophilus
parainfluenzae
Neisseria meningitidis 30 26-29 25 – – – – – – – – – – – –
S. pneumoniae 19 16-18 15 – – – – – – – – – – 20-28 –
Enterobacterales 14 11-13 11 23-29 – – – – – – – – – – –
Acinetobacter spp. 14 11-13 11 – – – – – – – – – – – –
Staphylococcus spp. 17 14-16 14 – – – – 26-32 – – – – – – –
Stenotrophomonas maltophila 50 17-49 16 – – – – – – – – – – – –
Enterococcus spp. 50 21-49 21 – – – – – 26-34 – – – – – –
Streptococcus group A, B, C & G,
18 15-17 15 – – – – – – – – – – – –
Moraxella catarrhalis
S.pneumoniae 13 10-12 10 – – – – – – – – – – 18-26 –
Haemophilus influenzae 23 20-22 20 – – – – – – – 27-35 – – – –
Listeria monocytogens 29 – 29 – – – – – – – – – – – –
Pasteurella multocida 23 – 23 – – – – – – – – – – – –
Kingella kingae 28 – 28 – – – – – – – – – – – –
Aeromonas spp. 19 16-18 16 – – – – – – – – – – – –
Burkholderia pseudomallei 50 17-49 17 – – – – – – – – – – – –
Achromobacter xylosoxidans 26 – 26 – – – – – – – – – – – –
Vibrio spp. 21 – 21 – – – – – – – – – – – –
# SD283 Doripenem DOR 10 mcg
Enterobacterales 23 20-22 19 27-35 33-42 – – – – – – – – 30-38 –
P. aeruginosa 19 16-18 15 – – 28-35 – – – – – – – – –
Haemophilus influenzae &
16 – – – – – – – – 21-31 – – – – –
Haemophilus parainfluenzae
Acinetobacter spp. 18 15-17 14 – – – – – – – – – – – –
Enterobacterales 24 21-23 21 – – – – – – – – – – 31-37 –
P. aeruginosa 50 22-49 22 – – 28-35 – – – – – – – – –
Acinetobacter 50 22-49 22 – – – – – – – – – – – –
SD012 Doxycycline Hydrochloride DO 30 mcg
Enterobacterales 14 11-13 10 18-24 23-29 – – – – – – – – – –
Acientobacter 13 10-12 9 – – – – – – – – – – – –
Staphylococcus & Enterococcus spp. 16 13-15 12 – – – – – – – – – – – –
S. pneumoniae 28 25-27 24 – – – – – – – – – – 25-34 –
SD237 Enoxacin EN 10 mcg
Enterobacterales 18 15-17 14 28-36 22-28 22-28 – – – – – – 43-51 – –
Staphylococcus spp. 18 15-17 14 – – – – – – – – – – – –
Neisseria gonorhoeae 36 32-35 31 – – – – – – – – – – – –
SD280 Ertapenem ETP 10 mcg
Enterobacterales 22 19-21 18 29-36 – 13-21 – – – – 27-33 – – 28-35 –
Staphylococcus spp. 19 16-18 15 – 24-31 – – – – – – – – – –
Haemophilus influenzae & parainfluenzae 19 – – – – – – – – 20-28 – – – – –
Enterobacterales 25 – 25 29-36 – – – – – – – – – 28-34 –
Haemophilus influenzae 23 – 23 – – – – – – – 27-33 – – – –
Moraxella catarrhalis 29 – 29 – – – – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
# : Doripenem QC limits are as per EUCAST, version - 8 (2018) & have been deleted in version 9 (2019).
6 · : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD013 Erythromycin E 15 mcg
Staphylococcus & Enterococcus spp. 23 14-22 13 – 22-30 – – – – – – – – – –
S. pneumoniae, Streptococcus spp.
Viridians group, Streptococcus spp. 21 16-20 15 – – – – – – – – – – 25-30 –
beta haemolytic group
Staphylococcus spp. &
21 – 21 – – – – 23-29 – – 10-16 – – – –
Streptococcus group A, B, C & G
S.pneumoniae 22 – 22 – – – – – – – – – – 26-32 –
Moraxella catarrhalis 23 – 23 – – – – – – – – – – – –
Listeria monocytogens 25 – 25 – – – – – – – – – – – –
Campylobacter coli 24 – 24 – – – – – – – – – – – –
Campylobacter jejuni 20 – 20 – – – – – – – – – – – 27-35
Kingella kingae 20 – 20 – – – – – – – – – – – –
Bacillus spp. 24 – 24 – – – – – – – – – – – –
Vibrio spp. 12 – 12 – – – – – – – – – – – –
SD279 Faropenem FAR 5 mcg
Enterobacterales – – – 20-26 27-34 – – – – 15-22 – – – 27-35 –
SD205 Fosfomycin FO 200 mcg
Enterobacterales, Enterococcus spp. 16 13-15 12 22-30 25-33 – – – – – – – – – –
Enterobacterales (IV) 24 – 24 26-34 – – – – – – – – – – –
Enterobacterales (uncomplicated UTI
21 – 21 – – – – – – – – – – – –
only E. coli)
SD171 Fusidic acid FC 10 mcg – – – – 24-32 – – – – – – – – 9-16 –
Staphylococcus spp. 24 – 24 – – – – 26-32 – – – – – – –
SD737 Gatifloxacin GAT 5 mcg
Enterobacterales, P. aeruginosa,
18 15-17 14 30-37 – 20-28 – – – – – – – – –
Acientobacter & Enterococcus spp.
Staphylococcus spp. 23 20-22 19 – 27-33 – – – – – – – – – –
Haemophilus influenzae &
18 – – – – – – – – 33-41 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 23 20-22 19 – – – – – – – – – 45-56 – –
S. pneumoniae, Streptococcus spp.
Viridians group, Streptococcus spp. 21 18-20 17 – – – – – – – – – – 24-31 –
beta haemolytic group
SD250 Gemifloxacin GEM 5 mcg
Enterobacterales 20 16-19 15 29-36 27-33 19-25 – – – – – – – – –
Haemophilus influenzae &
18 – – – – – – – – – – – – – –
Haemophilus parainfluenzae
S. pneumoniae 23 20-22 19 – – – – – – – – – – 28-34 –
SD195 Gentamicin HLG 120 mcg
Enterococcus spp. 23 20-22 19 – 27-33 – – – – – – – – – –
SD016 Gentamicin GEN 10 mcg
Enterobacterales, P. aeruginosa,
15 13-14 12 19-26 19-27 17-23 – – – – – – – – –
Acientobacter & Staphylococcus spp.
Enterobacterales 17 – 17 19-26 – – – – – – – – – – –
Staphylococcus spp. 18 – 18 – – – – 19-25 – – – – – – –
Coagulase negative Staphylococci 22 – 22 – – – – – – – – – – – –
Pseudomonas spp. 15 – 15 – – 17-23 – – – – – – – – –
Acinetobacter spp. 17 – 17 – – – – – – – – – – – –
Corynebacterium spp. 23 – 23 – – – – – – – – – – – –
SD170 Gentamicin GEN 30 mcg
Enterococcus spp. – – – – – – – – 12-18 – – – – – –
SD073 Imipenem IPM 10 mcg
Enterobacterales 23 20-22 19 26-32 – – – – – – – 25-33 – – –
P. aeruginosa 19 16-18 15 – – 20-28 – – – – – – – – –
Haemophilus influenzae &
16 – – – – – – – – 21-29 – – – – –
Haemophilus parainfluenzae
Acientobacter spp. 22 19-21 18 – – – – – – – – – – – –
Enterobacterales 22 17-21 17 26-32 – – – – – – – – – 34-42 –
Pseudomonas spp. 50 21-49 20 – – 20-28 – – – – – – – – –
Acinetobacter spp. 24 21-23 21 – – – – – – – – – – – –
Enterococcus spp. 50 22-49 21 – – – – – 24-30 – – – – – –
Haemophilus influenzae 20 – 20 – – – – – – – 24-30 – – – –
Moraxella catarrhalis 29 – 29 – – – – – – – – – – – –
Morganella morganii, Proteus spp. &
50 19-49 19 – – – – – – – – – – – –
Providencia spp.
Burkholderia pseudomallei 29 – 29 – – – – – – – – – – – –
Bacillus spp. 30 – 30 – – – – – – – – – – – –
SD017 Kanamycin K 30 mcg
Enterobacterales, Staphylococcus spp. 18 14-17 13 17-25 19-26 – – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
7
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD216 Levofloxacin LE 5 mcg
Enterobacterales, S. Typhi 21 17-20 16 29-37 – – – – – – – – – – –
P. aeruginosa 22 15-21 14 – – 19-26 – – – – – – – – –
Acientobacter spp.,
S. maltophila, Enterococcus spp.,
S. pneumoniae,
17 14-16 13 – – – – – – – – – – 20-25 –
Streptococcus spp. Viridians group,
Streptococcus spp.
beta haemolytic group
Staphylococcus spp. 19 16-18 15 – 25-30 – – – – – – – – – –
Haemophilus influenzae &
17 – – – – – – – – 32-40 – – – – –
Haemophilus parainfluenzae
Enterobacterales, 23 19-22 19 29-37 – – – – – – – – – – –
Staphylococcus spp., Pseudomonas spp. 50 23-49 22 – – 19-26 – 23-29 – – – – – – –
Coagulas-negative Staphylococci 50 25-49 24 – – – – – – – – – – – –
Enterococcus spp. (Uncomplecated UTI only) 15 – 15 – – – – – 19-25 – – – – – –
Acinetobacter spp. 23 20-22 20 – – – – – – – – – – – –
Streptococcus group A, B, C & G 50 18-49 17 – – – – – – – – – – – –
S.pneumoniae 50 17-49 16 – – – – – – – – – – 21-27 –
Haemophilus influenzae 30 – 30 – – – – – – – 31-39 – – – –
Moraxella catarrhalis 29 – 29 – – – – – – – – – – – –
Pasteurella multocida 27 – 27 – – – – – – – – – – – –
Kingella kingae 28 – 28 – – – – – – – – – – – –
Bacillus spp. 50 23-49 23 – – – – – – – – – – – –
Vibrio spp. 23 – 23 – – – – – – – – – – – –
SD854 Levonadifloxacin LND 10 mcg
Staphylococcus aureus 17 14-16 13 27-33 32-39 17-23 – – – 33-41 – – – 24-31 –
S. pyogenes 20 – 19 – – – – – – – – – – – –
E. faecalis 10 – 9 – – – – – – – – – – – –
S. dysgalactiae & S. agalactiae 20 – 19 – – – – – – – – – – – –
SD215 Linezolid LZ 30 mcg
Staphylococcus spp. 21 – 20 – 25-32 – – – – – – – – – –
Enterococcus spp. 23 21-22 20 – – – – – – – – – – – –
S. pneumoniae, Streptococcus spp.
Viridians group, Streptococcus spp. 21 – – – – – – – – – – – – 25-34 –
beta haemolytic group
SD296E Linezolid LZ 10 mcg
Enterococcus spp. 20 – 20 – – – – – 19-25 – – – – – –
Staphylococcus spp. 21 – 21 – – – – 21-27 – – – – – – –
Streptococcus group A, B, C & G 19 – 19 – – – – – – – – – – – –
Corynebacterium spp. 25 – 25 – – – – – – – – – – – –
S. pneumoniae 22 – 22 – – – – – – – – – – 23-29 –
Bacillus spp. 22 – 22 – – – – – – – – – – – –
SD206 Lomefloxacin LOM 10 mcg
Enterobacterales, P. aeruginosa &
22 19-21 18 27-33 23-29 22-28 – – – – – – – – –
Staphylococcus spp.
Haemophilus influenzae &
22 – – – – – – – – 33-41 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 38 27-37 26 – – – – – – – – – 45-54 – –
SD176 Mecillinam MEC 10 mcg
Enterobacterales 15 12-14 11 24-30 – – – – – – – – – – –
Enterobacterales
15 – 15 24-30 – – – – – – – – – – –
(Uncomplicated UTI only)
SD727 Meropenem MRP 10 mcg
Enterobacterales 23 20-22 19 28-35 – – – – – – – – – – –
P. aeruginosa 19 16-18 15 – – 27-33 – – – – – – – – –
Staphylococcus spp. 16 14-15 13 – 29-37 – – – – – – – – – –
B. cepacia 20 16-19 15 – – – – – – – – – – – –
Haemophilus influenzae &
20 – – – – – – – – 20-28 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 30 – – – – – – – – – – – – 28-35 –
Acientobacter spp. 18 15-17 14 – – – – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
8 On receipt all other products to be stored between -20°C to 8°C.
For prolonged use, store at or below -20°C.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
Enterobacterales (indications other than
22 16-21 16 28-35 – – – – – – – – – 30-38 –
meningitis)
Enterobacterales (meningitis) 22 – 22 – – – – – – – – – – –
Pseudomonas spp. (indications other
24 18-23 18 – – 27-33 – – – – – – – – –
than meningitis)
Pseudomonas spp. (meningitis) 22 – 22 – – – – – – – – – – – –
Acinetobacter spp. (indications other than
21 15-20 15 – – – – – – – – – – – –
meningitis)
Acinetobacter spp. (meningitis) 21 – 21 – – – – – – – – – – – –
Haemophilus influenzae 20 – 20 – – – – – – – 27-35 – – – –
Moraxella catarrhalis 33 – 33 – – – – – – – – – – – –
Listeria monocytogens 26 – 26 – – – – – – – – – – – –
Aerococcus sanguinicola & urinae 31 – 31 – – – – – – – – – – – –
Kingella kingae 30 – 30 – – – – – – – – – – – –
Burkholderia pseudomallei 24 – 24 – – – – – – – – – – – –
Bacillus spp. 25 – 25 – – – – – – – – – – – –
Achromobacter xylosoxidans 26 20-25 20 – – – – – – – – – – – –
#SD019 Methicillin MET 5 mcg – – – – 17-22 – – – – – – – – – –
#SD225 Mezlocillin MZ 75 mcg – – – 23-29 – 19-25 – – – – – – – – –
SD158 Minocycline MI 30 mcg
Enterobacterales, Acientobacter 16 13-15 12 19-25 25-30 – – – – – – – – – –
B. cepacia, S. maltophila,
19 15-18 14 – – – – – – – – – – – –
Staphylococcus & Enterococcus spp.
Neisseria meningitidis 26 – – – – – – – – – – – – – –
Staphylococcus spp., 23 – 23 – – – – 23-29 – – – – – – –
Streptococcus group A, B, C & G 23 – 23 – – – – – – – – – – – –
S.pneumoniae 24 – 24 – – – – – – – – – – 25-31 –
Haemophilus influenzae 24 – 24 – – – – – – – 26-32 – – – –
Moraxella catarrhalis 25 – 25 – – – – – – – – – – – –
SD220 Moxalactam MX 30 mcg
Enterobacterales 23 15-22 14 28-35 18-24 17-25 – – – – – – – – –
SD217 Moxifloxacin MO 5 mcg
Staphylococcus spp. 24 21-23 20 28-35 28-35 17-25 – – – – – – – – –
Haemophilus influenzae &
18 – – – – – – – – 31-39 – – – – –
Haemophilus parainfluenzae
S. pneumoniae 18 15-17 14 – – 25-31 –
Enterobacterales 22 – 22 28-35 – – – – – – – – – – –
Staphylococcus spp. 25 – 25 – – – – 25-31 – – – – – – –
Coagulase-negative Staphylococci 28 – 28 – – – – – – – – – – – –
Streptococcus group A, B, C & G 19 – 19 – – – – – – – – – – – –
S.pneumoniae 22 – 22 – – – – – – – – – – 24-30 –
Haemophilus influenzae, 28 – 28 – – – – – – – 30-36 – – – –
Corynebacterium spp. 25 – 25 – – – – – – – – – – – –
Moraxella catarrhalis 26 – 26 – – – – – – – – – – – –
SD293E Mupirocin MUP 200 mcg
Staphylococcus spp. – – – – – – – 31-37 – – – – – – –
SD246 Nafcillin NAF 1 mcg – – – – 16–22 – – – – – – – – – –
SD021 Nalidixic Acid NA 30 mcg
Enterobacterales 19 14-18 13 22-28 – – – – – – – – – – –
Haemophilus influenzae, 23 – – 22-28 – – – – – – 26-32 – – – –
Moraxella catarrhalis, 23 – – – – – – – – – – – – – –
Pasteurella multocida 23 – – – – – – – – – – – – – –
SD731 Neomycin N 10 mcg
S. pseudontermedius & S. schelfer 20 – 20 14-20 – – – 16-22 – – – – – – –
SD046 Netillin (Netilmicin Sulphate) NET 30 mcg
Enterobacterales, P. aeruginosa,
15 13-14 12 22-30 22-31 17-23 – – – – – – – – –
Staphylococcus spp.
SD085 Netillin (Netilmicin Sulphate) NET 10 mcg
Enterobacterales 15 12-14 12 18-24 – – – – – – – – – – –
Staphylococcus spp. 18 – 18 – – – – 20-26 – – – – – – –
Coagulase negative Staphylococci 22 – 22 – – – – – – – – – – – –
Pseudomonas spp. 12 – 12 – – 15-21 – – – – – – – – –
Acinetobacter spp. 16 – 16 – – – – – – – – – – – –
SD023 Nitrofurantoin NIT 300 mcg
Enterobacterales, Staphylococcus &
17 15-16 14 20-25 18-22 – – – – – – – – 23-29 –
Enterococcus spp.

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
# : QC limits are as per CLSI guideline (2018) & have been deleted in CLSI guideline (2019).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium; 9
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD086 Nitrofurantoin NIT 100 mcg
Enterobacterales (Uncomplecated UTI only) 11 – 11 17-23 – – – – – – – – – – –
Staphylococcus spp.
13 – 13 – – – – 17-23 – – – – – – –
(Uncomplecated UTI only)
Streptococcus group A, B, C & G
15 – 15 – – – – – – – – – – 25-31 –
(Uncomplecated UTI only)
Enterococcus spp. (Uncomplecated UTI only) 15 – 15 – – – – – 18-24 – – – – – –
Aerococcus sanguinicola & urinae 16 – 16 – – – – – – – – – – – –
SD196 Nitroxoline NO 30 mcg
Enterobacterales
15 – 15 18-24 – – – – – – – – – – –
(uncomplicated UTIs only)
#SD057 Norfloxacin NX 10 mcg
Enterobacterales, P. aeruginosa,
17 13-16 12 28-35 17-28 22-29 – – – – – – – 15-21 –
Staphylococcus & Enterococcus spp.
Enterobacterales spp.
24 – 24 28-35 – – – – – – – – – – –
(Uncomplecated UTI only)
Staphylococcus spp. 17 – – – – – – 18-24 – – – – – – –
Enterococcus spp. 12 – 12 – – – – – 16-22 – – – – – –
Streptococcus group A, B, C & G, 12 – – – – – – – – – – – – – –
S.pneumoniae 10 – – – – – – – – – – – – 18-24 –
Aerococcus sanguinicola & urinae 17 – 17 – – – – – – – – – – – –
*SD053 Novobiocin NV 30 mcg 22 18-21 17 – 22-31 – – – – – – – – – –
SD087 Ofloxacin OF 5 mcg
Enterobacterales, P. aeruginosa,
S. pneumoniae, Streptococcus spp.
16 13-15 12 29-33 – 17-21 – – – – – – – 16-21 –
Viridians group, Streptococcus spp.
beta haemolytic group
Staphylococcus spp. 18 15-17 14 – 24-28 – – – – – – – – – –
Haemophilus influenzae &
16 – – – – – – – – 31-40 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 31 25-30 24 – – – – – – – – – 43-51 – –
Enterobacterales 24 22-23 22 29-33 – – – – – – – – – 18-24 –
Staphylococcus spp. 50 20-49 20 – – – – 21-27 – – – – – – –
Coagulase-negative Staphylococci 50 24-49 24 – – – – – – – – – – – –
Haemophilus influenzae 30 – 30 – – – – – – – 31-37 – – – –
Moraxella catarrhalis 28 – 28 – – – – – – – – – – – –
SD088 Oxacillin OX 1 mcg
Staphylococcus (S. pseudintermedius) 18 – 17 – 18-24 – – – – – – – – – –
S. pneumoniae 20 – – – – – – – – – – – – <=12 –
S. pneumoniae 20 – – – – – – 19-25 – – – – – 8-14 –
SD070 Pefloxacin PF 5 mcg
Enterobacterales (S. Typhi) 24 – 23 25-33 – – – – – – – – – – –
Enterobacterales 24 – 24 26-32 – – – – – – – – – – –
Vibrio spp. 22 – 22 – – – – – – – – – – – –
SD028 Penicillin–G P 10 units
Staphylococcus spp. 29 – 28 – 26-37 – – – – – – – – – –
Enterococcus spp. 15 – 14 – – – – – – – – – – – –
Neisseria gonorhoeae 47 27-46 26 – – – – – – – – – 26-34 – –
Streptococcus spp.
24 – – – – – – – – – – – – 24-30 –
beta haemolytic group
SD089 Penicillin G P 1 unit
Staphylococcus spp. 26 – 26 – – – – 12-18 – – – – – – –
Streptococcus group A, B, C & G
18 – 18 – – – – – – – – – – 16-22 –
(indications other than meningitis)
Streptococcus group A, B, C & G
19 – 19 – – – – – – – – – – – –
(meningitis)
Streptococcus spp. viridans group 21 – 21 – – – – – – – – – – – –
Haemophilus influenzae 12 – – – – – – – – 6-9 15-21 – – – –
Listeria monocytogens 13 – 13 – – – – – – – – – – – –
Pasteurella multocida 17 – 17 – – – – – – – – – – – –
Corynebacterium spp. 29 – 29 – – – – – – – – – – – –
Aerococcus sanguinicola & urinae 21 – 21 – – – – – – – – – – – –
Kingella kingae 25 – 25 – – – – – – – – – – – –
#SD066 Piperacillin PI 100 mcg
Enterobacterales & Acientobacter spp. 21 18-20 17 24-30 – – 12-18 – – – – – – – –
P. aeruginosa 21 15-20 14 – – 25-33 – – – – – – – – –
SD066A Piperacillin PI 30 mcg
Enterobacterales 20 – 20 21-27 – – – – – – – – – – –
Pseudomonas spp. 50 18-49 18 – – – – – – – – – – – –
SD210 Piperacillin/ Tazobactam PIT 100/
Enterobacterales & Acientobacter spp. 10 mcg 25 21-24 20 24-30 27-36 – 24-30 – – – – – – – –
P. aeruginosa 21 15-20 14 – – 25-33 – – – – – – – – –
Haemophilus influenzae &
21 – – – – – – – – 33-38 – – – – –
Haemophilus parainfluenzae

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
* : Not included in CLSI chart; FDA approved performance standards for Antimicrobial Discs obtained from drug manufacturers.

10  :
# :
Deterioration in Oxacillin disc content is best assessed with QC Organism S. aureus ATCC 25923, with an acceptable zone diameter of 18-24 mm.
Interpretative criteria and QC limits are as per CLSI guideline (2018) & have been deleted in CLSI guideline (2019).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C.
For prolonged use, store at or below -20°C.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD292E Piperacillin / Tazobactam PIT 30/6 mcg
Enterobacterales 20 19 20 21-27 – – 21-27 – – – – 14-20 – – –
Pseudomonas spp. 50 18-49 18 – – 23-29 – – – – – – – – –
Haemophilus influenzae 27 – 27 – – – – – – – 32-40 – – – –
Achromobacter xylosoxidans 26 – 26 – – – – – – – – – – – –
Vibrio spp. 26 – 26 – – – – – – – – – – – –
SD029 Polymyxin–B PB 300 Units – – – 13-19 – 14-18 – – – – – – – – –
SD178 Pristinomycin
RP 15 mcg
(Quinupristin/Dalfopristin)
Staphylococcus, Enterococcus spp.,
S. pneumoniae, Streptococcus spp.
19 16-18 15 – 21-28 – – – – 15-21 – – – 19-24 –
beta haemolytic group &
Streptococcus spp. Viridians group
Staphylococcus spp. 21 – 21 – – – – 21-27 – – – – – – –
Enterococcus spp. 22 – 22 – – – – – 11-17 – – – – – –
SD030 Rifampicin RIF 5 mcg
Staphylococcus, Enterococcus spp., 20 17-19 16 8-10 – – – – – – – – – – –
Haemophilus influenzae &
20 17-19 16 – 26-34 – – – – 22-30 – – – – –
Haemophilus parainfluenzae
Neisseria meningitidis 25 20-24 19 – – – – – – – – – – – –
S. pneumoniae 19 17-18 16 – – – – – – – – – – 25-30 –
Staphylococcus spp. 30 – 30 – – – – 30-36 – – – – – – –
Streptococcus group A, B, C & G 21 15-20 15 – – – – – – – – – – – –
S. pneumoniae 22 17-21 17 – – – – – – – – – – 26-32 –
Haemophilus influenzae 18 – 18 – – – – – – – 21-27 – – – –
Corynebacterium spp. 30 25-29 25 – – – – – – – – – – – –
Aerococcus sanguinicola & urinae 25 – 25 – – – – – – – – – – – –
Kingella kingae 20 – 20 – – – – – – – – – – – –
SD162 Sparfloxacin SPX 5 mcg
Enterobacterales – – – 30-38 – 21-29 – – – 32-40 – – 43-51 – –
Staphylococcus, S. pneumoniae 19 16-18 15 – 27-33 – – – – – – – – 21-27 –
SD181 Spectinomycin SPT 100 mcg
Neisseria gonorhoeae 18 15-17 14 – – – – – – – – – 23-29 – –
SD236 Streptomycin HLS 300 mcg
Enterococcus spp. 10 7-9 6 – – – – – 14-20 – – – – – –
Enterococcus spp. – – – – – – – – 14-20 – – – – – –
SD031 Streptomycin S 10 mcg
Enterobacterales 15 12-14 11 12-20 14-22 – – – – – – – – – –
SD032 Sulphafurazole (Sulfisoxazole) SF 300 mcg
Enterobacterales & Staphylococcus 17 13-16 12 15-23 24-34 – – – – – – – – – –
SD213 Teicoplanin TEI 30 mcg
Enterococcus spp. 14 11-13 10 – 15-21 – – – – – – – – – –
Enterococcus spp., 16 – 16 – – – – – 15-21 – – – – – –
Streptococcus spp. viridans group 16 – 16 – – – – – – – – – – – –
Streptococcus group A, B, C & G 15 – 15 – – – – – – – – – – – –
S. pneumoniae 17 – 17 – – – – – – – – – – 18-24 –
SD037 Tetracycline TE 30 mcg
Enterobacterales, Acientobacter 15 12-14 11 18-25 – – – – – – – – – – –
Staphylococcus, Enterococcus spp. &
19 15-18 14 – 24-30 – – – – – – – – – –
Neisseria meningitidis
Haemophilus influenzae &
29 26-28 25 – – – – – – 14-22 – – – – –
Haemophilus parainfluenzae
Neisseria gonorhoeae 38 31-37 30 – – – – – – – – – 30-42 – –
S. pneumoniae, 28 25-27 24 – – – – – – – – – – 27-31 –
Streptococcus spp. beta haemolytic
23 19-22 18 – – – – – – – – – – – –
group & Viridians group
Staphylococcus spp. 22 – 22 – – – – 23-31 – – – – – – –
Streptococcus group A, B, C & G 23 – 23 – – – – – – – – – – – –
S.pneumoniae, Haemophilus influenzae 25 – 25 – – – – – – – 28-34 – – 28-34 –
Corynebacterium spp., 24 – 24 – – – – – – – – – – – –
Pasteurella multocida 24 – 24 – – – – – – – – – – – –
Campylobacter jejuni & coli 30 – 30 – – – – – – – – – – – 30-38
Moraxella catarrhalis 28 25-27 25 – – – – – – – – – – – –
Kingella kingae 28 – 28 – – – – – – – – – – – –
Burkholderia pseudomallei 50 23-49 23 – – – – – – – – – – – –
Vibrio spp. 20 – 20 – – – – – – – – – – – –
SD201 Ticarcillin / Clavulanic Acid TCC 75/10mcg
Enterobacterales & Acientobacter 20 15-19 14 24-30 – – 21-25 – – – – – – – –
P. aeruginosa 24 16-23 15 – – 20-28 – – – – – – – – –
Staphylococcus spp. 23 – 22 – 29-37 – – – – – – – – – –
Enterobacterales 23 20-22 20 24-30 – – 21-25 – – – – – – – –
Pseudomonas spp. 50 18-49 18 – – 20-28 – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
11
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.
Zone Size Interpretative Chart
Product Antimicrobial Agent Sym- Disc Interpretative Criteria Quality Control Limits (mm)
Code bol content
Sen- Inter- Resi- E. coli S. aureus P. aeruginosa E. coli S. aureus E. faecalis H. influenzae H. influenzae K. pneumoniae N. gonorrhoeae S. pneumoniae C. jejuni
sitive mediate stant ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC
mm mm mm 25922 25923 27853 35218 29213 29212 49247 49766 700603 49226 49619 33560
or or
more less
SD074 Ticarcillin TI 75 mcg – – – 24-30 – 21-27 6 – – – – – – – –
Enterobacterales – – – 24-30 – – – – – – – – – – –
SD278 Tigecycline TGC 15 mcg – – – 20-27 20-25 9-13 – – – 23-31 – – 30-40 23-29 –
Enterobacterales, Enterococcus spp. 18 – 18 20-27 – – – – 20-26 – – – – – –
Staphylococcus spp. 19 – 19 – – – – 19-25 – – – – – – –
Streptococcus group A, B, C & G 19 – 19 – – – – – – – – – – 24-30 –
SD044 Tobramycin TOB 10 mcg
Enterobacterales, P. aeruginosa,
15 13-14 12 18-26 19-29 20-26 – – – – – – – – –
Acientobacter & Staphylococcus spp.
Enterobacterales 16 – 16 18-26 – – – – – – – – – – –
Staphylococcus spp. 18 – 18 – – – – 20-26 – – – – – – –
Coagulase negative Staphylococci 22 – 22 – – – – – – – – – – – –
Pseudomonas spp. 18 – 18 – – 20-26 – – – – – – – – –
Acinetobacter spp. 17 – 17 – – – – – – – – – – – –
SD039 Trimethoprim TR 5 mcg
Enterobacterales, Staphylococcus spp. 16 11-15 10 21-28 19-26 – – – – – – – – – –
Enterobacterales (Uncomplecated UTI only) 15 – 15 21-28 – – – – – – – – – – –
Staphylococcus spp. 14 – 14 – – – – 22-28 – – – – – – –
Enterococcus spp. 50 21-49 21 – – – – – 24-32 – – – – – –
SD268 Ulifloxacin (Prulifloxacin) PRU 5 mcg – – – 32-38 20-26 27-33 – – – – – – – – –
SD045 Vancomycin VA 30 mcg – – – – 17-21 – – – – – – – – – –
Enterococcus spp. 17 15-16 14 – – – – – – – – – – – –
S. pneumoniae, Streptococcus spp.
beta haemolytic group & 17 – – – – – – – – – – – – 20-27 –
Streptococcus spp. Viridians group
SD155 Vancomycin VA 5 mcg
Enterococcus spp. 12 – 12 – – – – – 10-16 – – – – – –
Streptococcus group A, B, C & G 13 – 13 – – – – – – – – – – – –
S.pneumoniae 16 – 16 – – – – – – – – – – 17-23 –
Streptococcus spp. viridans group 15 – 15 – – – – – – – – – – – –
Corynebacterium spp. 17 – 17 – – – – – – – – – – – –
Aerococcus sanguinicola & urinae 16 – 16 – – – – – – – – – – – –
Bacillus spp. 10 – 10 – – – – – – – – – – – –

 : In accordance to Performance Standards for Antimicrobial Disk Susceptibility Tests, CLSI & EUCAST.
: Zone size interpretative criteria given in Red colour is as per EUCAST standard (The European Committee on Antimicrobial Susceptibility Testing).
· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
For E. coli, S. aureus, P. aeruginosa : Mueller Hinton Agar (MHA). For Haemophilus spp. : Haemophilus Test Medium;
For S. pneumoniae : Mueller Hinton Agar with 5% sheep blood; For N. gonorrhoeae : GC Agar Base with 1% defined growth supplement.

References:
1. Bauer, Kirby, Sherris and Turck, 1966, Am. J. Clin. Path., 45 : 493.
2. Clinical & Laboratory Standards Institute, Performance Standards for Antimicrobial Disk Susceptibility Tests, M100S, 33rd Ed., CLSI Vol.- 43 No.3, Mar-2023.
For more details refer to this volume. For more details refer to this volume.
3. EUCAST, Breakpoint tables for interpretation of MICs & zone diameters, version 13.0, valid from 01.01.2023.
4. Routine & extended internal quality control for MIC determination & Disc diffusion as recommended by EUCAST, version 13.0, valid from 01.01.2023.

Zone size interpretative chart for Antifungal agent

(Based on results obtained on Mueller Hinton Agar + 2% Glucose + 0.5mcg/ml Methylene Blue Dye Medium)
Code Antifungal Agent Symbol Disc content Zone diameter, Nearest Whole (mm) Quality Control Limits (mm)
Resistant S-DD* Susceptible C. albicans C. parapsilosis C. tropicalis C. krusei
mm or less mm or more ATCC 90028 ATCC 22019 ATCC 750 ATCC 6258
SD298 Caspofungin CAS 5 mcg — — — 18-27 14-23 20-27 19-26
SD232 Fluconazole FLC 25 mcg 14 15-18 19 28-39 22-33 26-37 —
SD277 Voriconazole VRC 1 mcg 13 14-16 17 31-42 28-37 — 16-25

* S-DD - Susceptible - Dose Dependent

References: 1) Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts; Approved Guidelines - Third Edition Vol.38 No.24, Dec - 2018 CLSI document M44-A2.
For more details refer to this volume.
2) Zone Diameter Interpretive Standards, Corresponding Minimal Inhibitory Concentration (MIC) Interpretive Breakpoints, and Quality Control Limits for Antifungal Disk Diffusion Susceptibility
Testing of Yeasts, Third Informational Supplement CLSI document – M44-S3 – Aug 2009.

12
Zone Size Interpretative Chart
Diameter of zone of inhibition in (mm)
Product Disc content Quality Control Limits
Antimicrobial Agent Symbol
Code (mcg) Escherichia coli Pseudomonas aeruginosa Klebsiella pneumoniae Klebsiella pneumoniae
ATCC 25922 ATCC 27853 ATCC BAA 1144 ATCC 700063
SD860 Meropenem/EDTA MRE 10/750 28-35 27-33 – –
SD299 Meropenem/ Phenyl Boronic Acid MRB 10/200 28-35 27-33 – –
SD300 Meropenem/Cloxacillin MCL 10/200 28-35 27-33 – –
SD301 Cefotaxime/Cloxacillin CTC 30/200 29-35 – ≥28 –
SD302 Ceftazidime/Cloxacillin CZC 30/200 25-32 – ≥25 –
SD303 Cefotaxime/Clavulanic acid/ Cloxacillin CCC 30/200/200 29-35 – ≥28 ≥28

Diameter of zone of inhibition in (mm)

Kit V for ESBL Identification Disc Quality Control Limits
SD304 (As per EUCAST) Symbol content
(mcg) Escherichia coli Klebsiella pneumoniae
ATCC 25922 ATCC 700063
SD040 Cefotaxime CTX 30 29-35 17-25
SD724 Cefotaxime/Clavulanic acid CEC 30/10 30-37 ≥28
SD062 Ceftazidime CAZ 30 25-32 10-18
SD207 Ceftazidime/Clavulanic acid CAC 30/10 27-34 ≥23
SD219 Cefepime CPM 30 31-37 16-22
SD234 Cefepime/Clavulanic acid CFC 30/10 32-40 ≥27

Diameter of zone of inhibition in (mm)

Kit VI for ESBL Identification Disc Quality Control Limits
SD305 (As per CLSI) Symbol content
(mcg) Escherichia coli Klebsiella pneumoniae
ATCC 25922 ATCC 700063
SD040 Cefotaxime CTX 30 29-35 17-25
SD724 Cefotaxime/Clavulanic acid CEC 30/10 30-37 ≥28
SD062 Ceftazidime CAZ 30 25-32 10-18
SD207 Ceftazidime/Clavulanic acid CAC 30/10 27-34 ≥23

Diameter of zone of inhibition in (mm)

Disc Quality Control Limits
SD306 AmpC Detection Kit Symbol content
(mcg) Escherichia coli Klebsiella pneumoniae Klebsiella pneumoniae
ATCC 25922 ATCC BAA 1144 ATCC 700063
SD040 Cefotaxime CTX 30 29-35 – 17-25
SD301 Cefotaxime/Cloxacillin CTC 30/200 29-35 ≥28 –
SD062 Ceftazidime CAZ 30 25-32 – –
SD302 Ceftazidime/Cloxacillin CZC 30/200 25-32 ≥25 –

Diameter of zone of inhibition in (mm)

Disc content Quality Control Limits
SD307 ESBL+AmpC Detection Kit Symbol
(mcg) Escherichia coli Klebsiella pneumoniae Klebsiella pneumoniae
ATCC 25922 ATCC BAA 1144 ATCC 700063
SD040 Cefotaxime CTX 30 29-35 – 17-25
SD724 Cefotaxime/Clavulanic acid CEC 30/10 30-37 – ≥28
SD301 Cefotaxime/Cloxacillin CTC 30/200 29-35 ≥28 –
SD303 Cefotaxime/Clavulanic acid/Cloxacillin CCC 30/200/200 29-35 ≥28 ≥28

Diameter of zone of inhibition in (mm)

Disc Quality Control Limits
SD308 KPC+MBL Detection Kit (As per EUCAST) Symbol content Increase in zone of diameter with the combination of inhibitor
(mcg)
KPC Positive MBL Positive AmpC+porin loss or efflux
SD727 Meropenem MRP 10 R R R
SD299 Meropenem + Phenylboronic acid MRB 10/200 ≥ 4 mm < 4 mm ≥ 4 mm
SD300 Meropenem/Cloxacillin MCL 10/200 < 5 mm < 5 mm ≥ 5 mm
SD860 Meropenem/EDTA MRE 10/750 < 5 mm ≥ 5 mm < 5 mm

13
Quality Control Limits for Antibioticsp
Based on Results obtained using Mueller Hinton Agar
Diameter of zone of inhibition in mm Diameter of zone of inhibition in mm

Quality Control Limits Quality Control Limits

Product Sym- Disc Product Sym- Disc
Antimicrobial Agent Antimicrobial Agent
Code bol content Code bol content
E. coli S. aureus P. aeruginosa E. coli S. aureus P. aeruginosa
ATCC ATCC ATCC ATCC ATCC ATCC
25922 25923 27853 25922 25923 27853

SD082 Amikacin AK 10 mcg 16-23 18-24 15-23 SD310 Cefotaxime/Sulbactam CXS 30/10 mcg 29-35 25-31 18-22

SD001 Amoxicillin AMX 10 mcg 19-25 28-36 — K. pneumoniae ATCC 700603= 22-28
Acinetobacter baumannii
SD129 Amoxicillin AMX 25 mcg 23-30 28-36 — ATCC 19606 = 26-32

SD076 Amoxicillin AMX 30 mcg 25-32 28-36 — SD738 Cefpirome CFP 30 mcg 28-34 29-37 23-29

SD281 Amoxyclav (Amoxicillin/ AMC 50/10 mcg 24-30 30-38 — SD235 Cefpirome/Clavulanic acid CPC 30/7.5 mcg 29-35 29-37 17-23
Clavulanic acid)
SD160 Cefradine (Cephradine) CH 25 mcg 17-22 29-37 —
SD264 Amoxicillin/Sulbactam AMS 30/15 mcg 28-38 32-43 —
SD704 Cefradine (Cephradine) CH 30 mcg 17-22 29-37 —
SD078 Amoxyclav AMC 10 mcg 19-25 28-36 —
SD207 Ceftazidime/Clavulanic acid CAC 30/10 mcg 27-34 K. pneumoniae
SD077 Ampicillin AMP 25 mcg 20-30 32-40 — ATCC 700603 = > 23mm

SD113 Ampicillin/Cloxacillin AX 10 mcg 16-22 35-37 — SD269 Ceftazidime/Tazobactam CAT 80/10 mcg 25-32 19-28 23-30

SD124 Azithromycin AZM 30 mcg — 24-30 — SD252 Ceftazidime/Tazobactam CAT 30/10 mcg 25-32 17-24 22-29

SD094 Azlocillin AZ 30 mcg — — 22-28 SD109 Ceftriaxone CTR 10 mcg 29-35 22-28 17-23

SD263 Aztreonam AT 50 mcg 29-37 — 24-33 SD261 Ceftriaxone/Sulbactam CIS 30/15 mcg 31-37 24-30 16-21

SD003 Bacitracin B 10 units — 12-22 — SD256 Ceftriaxone/Tazobactam CIT 30/10 mcg 29-35 24-32 17-24

SD105 Bacitracin B 8 units — 12-22 — SD251 Ceftriaxone/Tazobactam CIT 80/10 mcg 29-35 24-32 17-24

SD825 Biapenem BPM 10 mcg 27-35 32-40 28-36 SD311 Cefuroxime/Clavulanic Acid CCV 30/10 mcg 20-26 27-35 —

E.faecalis ATCC 29212 = 21-28 K. pneumoniae ATCC 700603= 16-21

K. pneumoniae ATCC 700603 = 25-32
SD081 Chloramphenicol C 10 mcg 17-25 19-26 —
SD079 Cefaloridine (Cephaloridine) CR 10 mcg 17-21 29-37 —
SD131 Chloramphenicol C 50 mcg 23-29 25-32 —
SD005 Cefaloridine (Cephaloridine) CR 30 mcg 17-21 29-37 —
SD153 Chloramphenicol C 25 mcg 21-27 23-30 —
SD262 Cefepime CPM 50 mcg 32-40 26-34 27-35
SD007 Chlortetracycline CT 30 mcg 18-25 19-28 —
SD234 Cefepime/Clavulanic acid CFC 30/10 mcg 32-40 24-30 25-31
SD080 Ciprofloxacin CIP 10 mcg 30-40 27-35 28-35
SD247 Cefepime/Tazobactam CPT 80/10 mcg 31-39 30-35 27-34
SD142 Ciprofloxacin CIP 30 mcg 30-40 27-35 28-35
SD257 Cefepime/Tazobactam CPT 30/10 mcg 33-39 24-30 26-32
SD060A Ciprofloxacin CIP 1 mcg 26-36 20-28 22-30
SD820 Cefixime CFM 10 mcg 24-29 — —
SD164 Clindamycin CD 10 mcg — 28-34 —
SD266 Cefixime/Clavulanic acid CMC 5/10 mcg 24-32 — —
SD008 Cloxacillin COX 1 mcg — 18-24 —
SD203 Cefoperazone/Sulbactam CFS 75/30 mcg 27-33 23-30 23-29
SD075 Cloxacillin COX 5 mcg — 18-30 —
K. pneumoniae ATCC 700603= 24-30
SD143 Cloxacillin COX 10 mcg — 23-34 —
SD254 Cefoperazone/Sulbactam CFS 75/10 mcg 27-33 23-30 23-29
SD165 Cloxacillin COX 30 mcg — 30-40 —
K. pneumoniae ATCC 700603= 19-27
SD284 Cloxacillin COX 200 mcg — 36-50 —
SD259 Cefoperazone/Sulbactam CFS 50/50 mcg 28-36 24-33 22-29
SD009 Colistin (Methane Sulphonate) CL 10 mcg 11-15 — 11-15
K. pneumoniae ATCC 700603= 26-32
SD108 Colistin (Methane Sulphonate) CL 25 mcg 13-18 — 13-18
SD253 Cefoperazone/Tazobactam CST 75/10 mcg 27-32 23-30 22-28
SD097 Colistin (Methane Sulphonate) CL 50 mcg 15-20 — 15-20
SD040A Cefotaxime (Cephotaxime) CTX 10 mcg 29-35 25-31 18-22
SD297 Colistin Sulphate CS 10 mcg 11-17 — 11-17
SD285 Cefoxitin/Cloxacillin CXX 30/200 mcg 26-34 36-50 —
SD071 Co-Trimazine (Human) CM 25 mcg 21-28 19-26 —
SD724 Cefotaxime/Clavulanic acid CEC 30/10 mcg 30-37 29-36 —
SD052 Dicloxacillin D/C 1 mcg — 18-30 —

SD120 Doxycycline Hydrochloride DO 10 mcg 12-18 23-29 —

14 p : Concentration of Antibiotics not as per CLSI & not as per EUCAST.

· : On receipt, store at -20°C.
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
Quality Control Limits for Antibioticsp
Based on Results obtained using Mueller Hinton Agar
Diameter of zone of inhibition in mm Diameter of zone of inhibition in mm

Quality Control Limits Quality Control Limits

Product Sym- Disc Product Sym- Disc
Antimicrobial Agent Antimicrobial Agent
Code bol content Code bol content
E. coli S. aureus P. aeruginosa E. coli S. aureus P. aeruginosa
ATCC ATCC ATCC ATCC ATCC ATCC
25922 25923 27853 25922 25923 27853

SD150 Enrofloxacin EX 10 mcg 30-40 22-30 — SD121 Novobiocin NV 5 mcg — 22-31 —

SD156 Enrofloxacin EX 5 mcg 30-40 22-30 — SD069 Ofloxacin OF 2 mcg 29-33 24-28 17-21

SD083 Erythromycin E 10 mcg — 22-30 — SD026 Oleandomycin OL 15 mcg — 19-28 —

SD222 Erythromycin E 5 mcg — 22-30 — SD043 Oxacillin OX 5 mcg — 27-35 —

SD140 Floxidin FL 20 mcg 30-40 25-30 — SD027 Oxytetracycline O 30 mcg 18-25 19-28 —

SD141 Floxidin FL 30 mcg 30-40 25-30 — SD144 Penicillin G P 2 units — 26-37 —

SD179 Fosfomycin FO 50 mcg 19-25 25-33 — SD175 Pipemidic Acid PA 30 mcg 18-25 13-19 11-16

SD014 Framycetin F 100 mcg 18-24 18-24 16-21 SD185 Pipemidic Acid PA 20 mcg 18-25 13-19 11-16

SD015 Furazolidone FR 50 mcg 20-25 18-22 — SD132 Piperacillin PI 75 mcg 24-30 — 25-33

SD197 Furazolidone FR 100 mcg 20-25 18-22 — SD106 Polymyxin B PB 50 units 12-16 — 11-17

SD042 Furoxone FX 100 mcg 20-25 18-22 — SD139 Polymyxin B PB 100 units 12-16 — 11-17

SD169 Fusidic Acid FC 30 mcg — 26-37 — SD267 Prulifloxacin (Ulifloxacin) PRU 10 mcg 28-34 19-27 24-32

SD166 Gentamicin GEN 50 mcg 21-28 25-33 20-25 SD096 Rifampicin RIF 2 mcg 8-10 26-34 —

SD753 Gatifloxacin GAT 10 mcg 30-37 27-33 20-28 SD127 Rifampicin RIF 30 mcg 9-12 32-40 —

SD740 Gatifloxacin GAT 30 mcg 32-40 31-37 24-32 SD128 Rifampicin RIF 15 mcg 8-10 26-34 —

SD265 Imipenam/Cilastin IC 10/10 mcg 28-35 — 24-31 SD126 Roxithromycin RO 30 mcg — 22-30 —

SD282 Imipenam/EDTA IE 10/750 mcg 25-31 — 19-27 SD059 Sisomicin SS 10 mcg 17-24 19-26 17-22

SD214 Isepamicin IP 30 mcg 20-28 24-32 19-24 SD054 Spiramycin SR 30 mcg — 22-29 —

SD223 Kanamycin K 5 mcg 16-22 19-26 — SD101 Spiramycin SR 100 mcg — 22-29 —

SD018 Lincomycin L 2 mcg — 15-22 — SD091 Streptomycin S 25 mcg 15-23 17-25 —

SD084 Lincomycin L 10 mcg — 15-22 — SD056 Sulfasomidine SO 300 mcg 18-26 24-34 —

SD098 Lincomycin L 15 mcg — 22-32 — SD033 Sulphamethizole SM 300 mcg 18-26 24-34 —

SD125 Lomefloxacin LOM 30 mcg 27-33 23-29 22-28 SD055 Sulphamethoxypyridazine ST 300 mcg 18-26 24-34 —

SD260 Lomefloxacin LOM 15 mcg 28-36 24-32 22-30 SD036 Sulphaphenazole SP 200 mcg 18-26 24-34 —

SD177 Mecillinam MEC 25 mcg 24-36 — — SD092 Sulphadiazine SZ 100 mcg 12-20 16-26 —

SD068 Methanamine Mandalate ME 3 mg 13-18 14-22 — SD034 Sulphadiazine SZ 300 mcg 18-26 24-34 —

SD136 Methicillin MET 10 mcg — 17-22 — SD133 Tetracycline TE 10 mcg 18-25 24-30 —

SD137 Methicillin MET 30 mcg — 22-32 — SD154 Tobramycin TOB 30 mcg 18-26 19-29 19-25

SD748 Mupirocin MUP 5 mcg — 18-24 — SD135 Trimethoprim TR 1.25 mcg 12-18 10-16 —

SD258 Nadifloxacin NAD 5 mcg 28-34 29-37 25-32 E. faecalis ATCC 29212 = 14-22
S. aureus ATCC 29213 = 12-18
SD022 Neomycin N 30 mcg 17-23 18-26 —
SD093 Trimethoprim TR 10 mcg 21-28 19-26 —
SD103 Nitrofurantoin NIT 30 mcg 15-20 18-22 —
SD148 Trimethoprim TR 25 mcg 21-28 19-26 —
SD023A Nitrofurantoin NIT 50 mcg 15-21 14-20 —
SD149 Trimethoprim TR 30 mcg 21-28 19-26 —
SD090 Nitrofurantoin NIT 200 mcg 20-25 18-22 —
SD038 Triple Sulpha S3 300 mcg 15-23 24-34 —
SD024 Nitrofurazone NR 100 mcg 20-25 18-22 —
SD199 Tylosine TL 15 mcg — 22-30 —
SD184 Norfloxacin NX 5 mcg 28-35 17-28 22-29
SD163 Vancomycin VA 10 mcg — 17-21 —
SD053 Novobiocin NV 30 mcg — 22-31 —
SD182 Virginamycin VI 15 mcg — 22-30 —

p : Concentration of Antibiotics not as per CLSI & not as per EUCAST.

· : On receipt, store at -20°C.
15
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C.
Quality Control Limits for Antibioticsp
Based on Results obtained using Mueller Hinton Agar
Cultural Response : Avaerage diameter of zone of inhibition observed on Brucella agar with Hemin and Vitamin K1 supplement with 5 % v/v sterile defibrinated sheep blood (M1039). Incubated
anaerobically at 35°C for 24 - 48 hours..

Quality Control Limits

Product Code Antimicrobial Agent Symbol Disc content
Clostridium perfringens ATCC 12924 Clostridium perfringens ATCC 13124 Bacteroides fragilis ATCC 25285
SD020 Metronidazole MT 5 mcg 26-34 26-34 38-44
SD099 Metronidazole MT 4 mcg 26-34 26-34 38-44
SD730 Metronidazole MT 50 mcg 30-38 30-38 38-46

ZONE SIZE INTERPRETATIVE CHART

Product Antimicrobial Disc Interpretative criteria Quality Control Limits*
Symbol
Code Agent content Sensitive Resistant Bacteroides fragilis ATCC 25285 Fusobacterium necrophorum ATCC 25286
SD837 Kanamycin K 1000 mcg  10 mm < 10 mm Resistant (< 10 mm zone) Sensitive ( 10 mm zone)

*: Expected diameter of zone of inhibition, as per Quality Control results obtained on Brucella Blood Agar w/ Hemin and Vitamin K1 (M1039).

Quality Control Limits for Antifungal Agent

(Based on results obtained on Mueller Hinton Agar + 2% Glucose + 0.5mcg/ml Methylene Blue Dye Medium)
Quality Control Limits
Product Antimicrobial Disc C. albicans C.parapsilosis C.tropicalis C.krusei C.albicans S.cerevisiae
Symbol
Code Agent content ATCC ATCC ATCC ATCC ATCC ATCC
90028 22019 750 6258 10231 9763
SD111 Amphotericin-B AP 100 units 10-17 11-20 8-12 9-14 10-18 11-18
SD233 Amphotericin-B AP 20 mcg 10-15 10-17 8-10 8-12 10-16 8-12
SD270 Amphotericin-B AP 50 mcg 12-15 13-17 13-17 14-20 15-23 16-25
SD115 Clotrimazole CC 10 mcg 18-32 16-30 10-20 14-24 12-18 17-25
SD114 Fluconazole FLC 10 mcg 27-38 22-33 16-25 — 18-22 —
SD221 Itraconazole IT 10 mcg 16-20 11-18 8-13 8-15 18-22 —
SD276 Itraconazole IT 30 mcg 18-22 20-24 11-18 8-15 18-22 —
SD224 Ketoconazole KT 10 mcg 20-32 14-29 17-28 10-14 18-22 —
SD275 Ketoconazole KT 30 mcg 32-36 26-32 26-32 19-26 31-40 —
SD274 Ketoconazole KT 50 mcg 37-45 36-44 27-34 19-26 31-40 —
SD273 Miconazole MIC 30 mcg 22-26 13-17 14-20 19-26 20-27 20-28
SD272 Miconazole MIC 50 mcg 26-32 23-29 14-20 19-26 20-27 20-28
SD025 Nystatin NS 100 units 19-27 16-25 16-21 15-20 15-23 17-25
SD271 Nystatin NS 50 mcg 19-23 19-23 13-17 19-26 16-25 22-27

Limitation of Test: 7. When testing Vancomycin against Enterococci, plates should be held a full 24 hrs & examined
These discs are used for semi-quantitative in vitro susceptibility testing by the agar disc diffusion using transmitted light; the presence of a haze or any growth within the zone of inhibition
test. The values obtained by this test can be considered as a guide to therapy selection only after indicates resistance.
taking into consideration several other factors; and must be the sole decision and responsibility 8. Ofloxacin susceptible S. pneumoniae will also be susceptible to Levofloxacin.
of the physician along with the clinical experience in treating the infection. These tests are 9. Susceptibility & resistance to Azithromycin, Clarithromycin & Dirithromycin can be predicted
comparable to the standards as per the given specifications and set of experiment standards as by using Erythromycin for Streptococci.
far as possible. Please refer to CLSI & EUCAST standards for detailed limitation of susceptibility
test on the clinical use of an antibiotic in various therapeutic conditions. Refrences:
1. Bauer A. W., Kirby W.M., Sherris J.C. & Turck M. 1966, Am. J. Clin. Pathol., 45:493.
Note: 2. Bryskier A., Antimicrobial agents. 2005, ASM Press, USA.
1. Ampicillin disc is used for testing susceptibility to Amoxycillin as well. 3. Clinical & Laboratory Standards Institute, Performance Standards for Antimicrobial Disk
2. Cephalothin disc is used for testing susceptibility to Cephapirin, Cephaloridine, Cephalexin, Susceptibility Tests, M100S, 33rd Ed., CLSI Vol.- 43 No.3, Mar-2023. For more details
Cefaclor, Cephoxitin, Cefazolin, Cephradine and Cephadroxil as well. refer to this volume.
3. The Sulfisoxazole (Sulphafurazole) disc can be used to represent any of the currently available 4. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition, o
Vol. 1, Section 2.
-20 C
sulfonamide preparations. 5. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition, Vol. 3, Section 15.
4. The category “Intermediate” should be reported. Infections with bacteria of intermediate 6. Jorgensen, J.H., Pfaller, M.A., Carroll,
OnK.C., Funke,
receipt storeG.,
at Landry, M.L., Richter, S.S and
susceptibility may be considered moderately susceptible and may respond to antimicrobial Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
agents with a wide safe dosage range. 7. Lorian V., Antibiotics in Laboratory medicine. 2005, Lippincott Williams & Wilkins, USA.
5. Since certain strains of Providencia spp. have been reported to give false susceptible results IVD
8. Performance standards for Antifungal SusceptibilityIn vitro diagnostic nd
Testing of yeasts, 2 Ed., CLSI standard
with Cefprozil discs, strains of this genus should not be tested & reported with this disc. medical device
M60, June 2020.
6. All Staphylococcal isolates with zone diameters of 14 mm or less should be tested by an 23, Vadhani Industrial Estate,
9. Reddish G. F., 1929, J. Lab. Clin. Med., 14 : 649.LBS Marg,Mumbai-86,
MIC method.
10. EUCAST, Breakpoint tables for interpretation ofMS,India
MICs & zone diameters, version 13.0,
valid from 01.01.2023.
p : Concentration of Antibiotics not as per CLSI & not as per EUCAST. EC REP CEpartner4U ,
ESDOORNLAAN 13,
On receipt all other products to be stored between -20°C to 8°C. For prolonged use, store at or below -20°C. 3951DB MAARN, NL
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