EMILIO AGUINALDO COLLEGE

Congressional East Avenue, Burol Main, City of Dasmarinas, Cavite

PHILOSOPHY
Emilio Aguinaldo College is a private, non-sectarian, co-educational institution of learning that fosters equal and fair opportunities of education, the total development of a person, and one’s
national identity while conscious of his/her role in the global community.

VISION
Emilio Aguinaldo College envisions itself as an internationally recognized private non-sectarian, academic institution rooted in the Filipino nationalist tradition that consistently pursues the
advancement and welfare of humanity.

MISSION
Emilio Aguinaldo College provides an outcomes-based education with relevant curricula geared towards excellent research, active industry cooperation and sustainable community extension.

CORE VALUES
Virtue, Excellence and Service

INSTITUTIONAL OBJECTIVES

The objectives of Emilio Aguinaldo College are to:

1. offer opportunities for quality and relevant education to all;
2. cultivate the intellectual, spiritual, moral, social and physical aspects of a person;
3. instill appreciation of pride for one’s national identity;
4. produce graduates of global quality equipped with competencies in their fields of expertise.

QUALITY POLICY
Emilio Aguinaldo College commits to the continuous improvement of quality standards with emphasis on instruction, research and community service to benefit its stakeholders.

QUALITY OBJECTIVES
The objectives of Emilio Aguinaldo College are to:
a) adhere to all statutory and regulatory standards;
b) provide consistent quality service to students, parents and other stakeholders;
c) respond to periodic system review for continual improvement on quality standards.

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 INSTITUTIONAL
PROGRAM EDUCATIONAL OBJECTIVES
OBJECTIVES
The program objectives of EAC-C School of Medical Technology are to: a b c d
1. provide equal and fair opportunities of education to those who aspire to endeavor in Medical Technology and related fields.    
2. help Medical Technology students to acquire and develop skills, knowledge, and attitudes that will contribute to the overall social, mental,
   
and physical health of the community and country.
3. establish quality training and education in instrumentation, laboratory diagnosis, scientific research methods and processes critical in producing
   
graduates who are academically-equipped in facing the challenges of both national and global markets.
4. instill among students good moral values and appreciation for one’s national identity vital in the molding of highly disciplined, honest, socially
   
responsible, hard-working, dignified and self-reliant Medical Technologists.

I. Course Code: MCC3 31

Course Title: Analysis of Urine and Other Body Fluids
Pre-requisite: MPB1 21 - Human Anatomy and Physiology with Pathophysiology, MPB3 21 - Biochemistry for Medical Laboratory Sciences

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 Co-requisite: None
Credit: 3 units (2 units lecture, 1 unit laboratory)
Contact hours/week: 2 hours lecture, 3 hours laboratory

II. Course Description: The course deals with the study of the gross, chemical, and microscopic analyses of the different body fluids other than
blood. It includes the importance of these body fluids to body processes, the principles of the analytical procedures, interpretation of results and
clinical significance of the physiologically important substances found in these body fluids.

III. Program Outcomes in Relation to Program Educational Objectives:

Program
Program Outcomes
Educational
Objectives
A graduate of the Bachelor of Science in Medical Technology must attain: 1 2 3 4
A technical competence in the performance of clinical laboratory tests in aid of diagnosis, treatment, and management of diseases vis-à-vis
   
biosafety and waste management
B analytical and critical thinking skills in the workplace    
C the ability to engage in the collection, analysis, and projection of health information for improving the health care management system    
D inter-personal skills, leadership qualities, and ethical practice of the profession    
E the ability to apply research skills in relevant areas of Medical Technology/Medical Laboratory Science practice    
F participation in community-oriented activities    
G knowledge and experience from life-long learning activities    
H the ability to demonstrate effective teaching and communication skills    

IV. Course Objectives in Relation to Program Outcomes

Course Objectives PROGRAM OUTCOMES

Upon completion of the course, the student must be able to:
A B C D E F G H
1. explain the principles and mechanism involved in the formation, composition and
       
general characteristics of all body fluids (except blood) and secretions
2. perform skillfully the routine and special laboratory methods employed in the
       
proper handling, examination and disposal of different body fluids and secretions.
3. appreciate the importance of accurate and precise laboratory findings as aid to
       
the diagnosis and treatment of diseases.
4. manifest the following values: integrity, honesty, critical thinking, empathy and
       
value for life. Page 3 of 17
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 V. Course Outcomes in Relation to Course Objectives and Program Outcomes
Course Outcomes Course PROGRAM OUTCOMES
A student completing this course should at the minimum be Objectives
able to: 1 2 3 4 A B C D E F G H
1 2 3 4

1. Understand and relate the vital roles of Analysis of Urine

  
and Other Body Fluids in the practice and development of Medical           

Technology profession.

2. Perform various laboratory techniques in Analysis of Urine

and Other Body Fluids in relation to Medical Technology    
       
profession with utmost accuracy and precision,    

3. Appreciate the value of Analysis of Urine and Other Body

   
Fluids principles and practices in the continual development of        
   
Medical Technology profession.
References:
Books:
1. McPherson, RA and Pincus, MR (2011). Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd Ed. Saunders, Elsevier.
2. Strasinger, SK (2001). URINALYSIS AND BODY FLUIDS 4TH ED. F.A. Davis & Co., Philadelphia.
3. Strasinger, SK and Di Lorenzo, MS (2008). Urinalysis and Body Fluids 5th Ed. F.A. Davis & Co., Philadelphia.
4. Ford, M.D. Clinical Toxicology, W.B. Saunders, 2001
5. Sacher,R.M. and McPherson, R.A. Widmann’s Clinical Interpretation of Laboratory Tests, 10th Ed., F.A. Davis Company, 1991

E-Books:

Journals:

E-journals:

Websites:

Course Requirements: Lecture Discussion

Quizzes
Case study
Assignment/Seatwork

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 Oral Recitation
Major Examination

Grading System: The following percentage distribution shall be followed:

PRELIM (PG) + MIDTERM (MG) + FINAL (FG) =

3
The following recommended periodical grade components for this course:
Class Standing: 40%
Major exams: 60%
Total: 100%

Base: 30
Passing grade: 3.0 (equivalent to 75%)

VI. Course Coverage:

Week Lesson/Topics Intended Learning Teaching Learning Activities Assessment Tasks (ATs) Instructional
Outcomes (ILOs) (TLAs) Resources

At the end of the unit, the

I. Introduction to Urinalysis Lecture Quizzes Textbooks
student should be able
Lecture: to: Class Discussion Long exams Reference materials
1. History and early practice 1. Appreciate the Film Viewing Oral Revalida Printed notes
on urinalysis importance of urine Case Study Performance exam Overhead projectors
2. Importance of urine analysis in the diagnosis Group Presentation Motivational readings
analysis of disease. Visual charts
3. QA/QC in the laboratory 2. Trace the beginning of Historical notes
urinalysis.
1 4. Laboratory safety
3. Discuss the
PRELIM 5. Competencies and competencies and
responsibilities of Medical responsibilities of
Technologists in the Clinical Medical Technologist in
Microscopy section. Clinical Microscopy

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 Laboratory: section.
1. Review and demonstration
of Guidelines and Laboratory
Safety Procedures.
2. Use and functions of
apparatus and materials in
the laboratory.
II. Urine Formation, Renal At the end of the unit, the Lecture Quizzes Textbooks
and Function tests. student should be able Class Discussion Long exams Reference materials
Lecture: to: Film Viewing Oral Revalida Printed notes
1. Discuss the
1. Anatomy of the kidney Case Study Performance exam Overhead projectors
physiological
2. Renal Physiology mechanisms of Group Presentation Motivational
1.1. Renal Blood Flow glomerular filtration, readings
1.2. Glomerular Filtration tubular reabsorption Visual charts
1.3. Tubular Reabsorption tubular secretion, and Laboratory manuals
1.4. Tubular Secretion renal blood flow. Colored atlas
2-3 2. Identify various
2. Renal Function Tests Refereed journals
laboratory procedures
2.1. Glomerular Filtration
used to evaluate the
Tests different renal functions.
2.2. Tubular Reabsorption 3.Discuss the
Test advantages and
2.3. Tubular Secretion and disadvantages of using
Renal Blood Flow Tests different substances for
2. Renal Diseases the measurement of
glomerular filtration
Lab: Clearance tests
4-5 III. Specimen Collection, At the end of the unit, the Lecture Quizzes Textbooks
Processing and Proper student should be able Class Discussion Long exams Reference materials
Handling of Urine to: Film Viewing Oral Revalida Printed notes
1. Discuss the
1. Patient preparation Case Study Performance exam OHP
physiological
2. Specimen Collection and mechanisms of Group Presentation Motivational
Disposal Guidelines glomerular filtration, readings
3. Urine Preservatives tubular reabsorption Visual charts
4. Changes in Unpreserved tubular secretion, and Slide projectors
urine renal blood flow.

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 5. Types of Urine Specimens 2. Identify various
5.1. Random Specimen laboratory procedures
5.2. First Morning Specimen used to evaluate the
different renal functions.
5.3. Fasting Specimen
3.Discuss the
5.4. 2-Hour Post-Prandial advantages and
Urine Specimen disadvantages of using
5.5. Glucose Tolerance Test different substances for
Specimen the measurement of
5.6. Timed Specimen glomerular filtration
5.7. Catheterized Specimen
5.8. Midstream Clean-Catch
Specimen
5.9. Suprapubic Aspiration
Specimen
5.10. Three-Glass Specimen
5.11. Pediatric Specimen
6. Criteria in Acceptance and
Rejection of sample
5-6 IV. Physical Examination of At the end of the unit, the Lecture Quizzes Textbooks
Urine student should be able Class Discussion Long exams Reference materials
1. Urine Color to: Film Viewing Recitation Printed notes
1. List common terms to
1.1. Normal Urine Color Case Study Practical exams Overhead projectors
report urine color,
1.2. Abnormal Urine Color appearance, specific Group Presentation Motivational
2. Urine Appearance/ gravity, and odor. readings
Transparency 2. Discuss the Visual charts
2.1. Normal Appearance significance and Laboratory manuals
2.2. Abnormal Appearance correlation of color, Colored atlas
3. Urine Specific Gravity appearance, specific Refereed journals
gravity and odor in the
3.1. Urinometer Method
diagnosis of disease.
3.2. Refractometer Method 3. Discuss the pathologic
4. Urine Odor and non-pathologic
5. Urine volume causes of abnormal
Laboratory: color, clarity, specific
1. Perform Physical tests: gravity, and odor of urine.

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 a. color 4. Perform physical /
b. appearance macroscopic examination
c. specific gravity of urine
d. odor
e. volume

6 PRELIM EXAMINATION

7-8 VI. Microscopic Examination At the end of the unit, the Lecture Quizzes Textbooks
MIDTERM of Urine student should be able to Class Discussion Long exams Reference materials
1. Significance of Microscopic 1. Discuss the Film Viewing Recitation Printed notes
importance of
Examination Demonstration Practical exams Overhead projectors
microscopic examination
2. Methods of Microscopic as part of the routine Group Presentation Motivational
examination urinalysis. Readings.
3. Sediment Constituents 2. Differentiate the Visual charts
3.1. Cellular elements different types of Laboratory manuals
3.1.1. Red Blood Cells microscope that can be Colored atlas
3.1.2. White Blood Cells utilized for microscopic Refereed journals
examination.
3.1.3. Epithelial Cells
3. Identify the different
3.2. Casts cellular constituents,
3.3. Crystals crystals, and casts in
3.3.1. Pathologic urine.
3.3.2. Non-pathologic 4. Perform microscopic
3.4. Others: examination of urine.
3.4.1. Bacteria 5. Correlate microscopic
results with pathologic
3.4.2. Yeast
conditions.
3.4.3. Parasites 6. Correlate physical and
3.4.4. Spermatozoa chemical examination
3.9. Mucus results with microscopic
3.10. Crystals observations and be able
3.11. Artifacts to recognize any
4. Renal Calculi discrepancies.
7. Discuss the Quality
5. Quality Assurance in
Assurance in Urinalysis
Urinalysis

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 Laboratory: laboratory.
1. Perform microscopic
examination
2. Identification of casts and
crystals
VII. Special Urinary 1. Explain the abnormal Lecture Quizzes Textbooks
Screening accumulation of Class Discussion Long exams Reference materials
Tests metabolites in the urine Film Viewing Recitation Printed notes
in terms of overflow and
Lecture: Demonstration Practical exams Overhead projectors
renal disorders.
1. Overflow versus Renal 2. Discuss the different Group Presentation Motivational
disorders metabolic disorders and readings
2. Amino Acid Disorders its clinical manifestations. Visual charts
2.1. Phenylalanine-Tyrosine 3. Explain the screening Laboratory manuals
2.2. Branched Chain Amino tests for the different Colored atlas
acid metabolic disorders. Refereed journals
9
2.3. Tryptophan disorders
2.4. Cystine disorders
3. Porphyrin Disorders
4. Mucopolysaccharide
Disorders
5. Purine Disorders
6. Other Screening Tests
Laboratory:
1. Special Urinary Screening
Tests
10 VIII. CEREBROSPINAL At the end of the unit, the Lecture Quizzes Textbooks
FLUID student should be able to Class Discussion Long exams Reference materials
Lecture: 1. Discuss the major Film Viewing Recitation Printed notes
functions of
1. Definition of Terms Demonstration Practical exams Overhead projectors
Cerebrospinal Fluid
2. Formation and Physiology 2. Discuss the proper Group Presentation Motivational
3. Importance of CSF collection, handling, and readings
Analysis processing of CSF Visual charts
4. Specimen Collection 3. Discuss the Laboratory manuals
4.1. Sites of collection hematological Colored atlas

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4.2. Methods of collection examinations done on Refereed journals
4.3. Precautions in CSF CSF
collection 4. Discuss the different
chemistry determinations
5. Transport, Handling,
done on a CSF sample.
Processing, Preservation, 5. Discuss the
and Disposal of samples. microbiological tests for
6. Specimen Acceptance and CSF
Rejection 6. Discuss the serological
7. Characteristics of CSF examinations on CSF.
8. Routine Examination
8.1. Macroscopic
8.2. Microscopic
8.3. Chemical: Routine and
Special
8.4. Gram Stain and Culture
8.5. Serologic Tests (e.g.
VDRL)
9. Reference values
10. Referral of Specimens
11. Clinical Significance:
Bacterial, Viral, Fungal
12. Sources of error
13. QA and QC
14. Reporting of Test Results
Laboratory:
1. Routine CSF Examination
Macroscopic
1.1.1. Color
1.1.2. Transparency
1.1.3. Presence of Clot
1.2. Microscopic
1.2.1. WBC Count
1.2.2. Differential Count
1.2.3. Corrected WBC Count

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 1.2.4. Corrected WBC count
1.3. Chemical
l.3.1. Glucose
1.3.2. Protein
1.3.3. Albumin
1.3.4. Globulin
1.4. India Ink stain
1.5. Gram Stain
1.6. Culture
1.7. Serology- VDRL, etc.
2. Laboratory Safety
11 IX. Seminal Fluid At the end of the unit, the Lecture Quizzes Textbooks
Examination student is able to: Class Discussion Long exams Reference materials
Lecture: 1. Define terms related to Film Viewing Recitation Printed notes
seminal fluid analysis
1. Definition of Terms Demonstration Practical exams Overhead projectors
2. Trace the formation of
2. Formation of Seminal Fluid seminal fluid Group Presentation Motivational
3. Patient Preparation 3. Discuss specimen readings
4. Specimen Considerations: considerations such as Visual charts
4.1. Collection collection, handling, Laboratory manuals
4.2. Handling transport, preservation Colored atlas
4.3. Transport and processing. Refereed journals
3. Discuss the protocols
4.4. Preservation
in specimen
4.5. Processing acceptability.
5. Specimen Acceptability 4. Perform properly
6. Routine Examination routine seminal fluid
6.1. Volume analysis.
6.2. Viscosity 5. Discuss the principles
6.3. pH in the different tests
performed in seminal
6.4. Viability
fluid.
6.5. Motility 6. Correlate pathological
6.6. Cell count and differential conditions related to test
count results.
6.7. Chemical Examinations 7. Report test results
7. Special Tests accurately.

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 8. Reference Values 8. Apply the principles of
9. Clinical Significance of quality assurance and
Seminal Fluid Analysis quality control.
10. QA and QC in Seminal
Analysis
11. Manner of Reporting
Laboratory:
1. Seminal fluid analysis
1.1. Patient preparation
1.2. Physical Examination
1.2.1. Color
1.2.2. Viscosity
1.2.3. Volume
1.3. Chemical examination
1.3.1. Glucose tests
1.3.2. Protein test
1.4. Microscopic examination
1.4.1. Sperm Count
1.4.2. Motility
1.4.3. Morphology
1.5. Additional Testing:
1.5.1. Microbiological tests
1.5.1.1. Gram stain
1.5.1.2. India Ink
1.5.1.3. Culture
1.5.2. Serological Tests
1.5.2.1. VDRL
2. Laboratory Safety

12 MIDTERM EXAMINATION

13 X. Pregnancy Test 1. Define terms related to Lecture Quizzes Textbooks

FINAL Lecture: Pregnancy test. Class Discussion Long exams Reference materials
1. Definition of terms 2. Discuss specimen Film Viewing Recitation Printed notes
considerations such as

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 2. Importance of Pregnancy collection, handling, Demonstration Practical exams Overhead projectors
Tests transport, preservation Group Presentation Motivational
3. Patient Preparation and processing. readings
3. Discuss the protocols
4. Specimen Considerations: Visual charts
in specimen
Collection acceptability. Laboratory manuals
Processing 4. Perform properly Colored atlas
Preservation pregnancy tests. Refereed journals
Handling 5. Discuss the principles
Transport in the different
5. Specimen Acceptability Pregnancy tests.
6. Correlate conditions
6. Different Tests for
related to test results.
Pregnancy 7. Report test results
7. Quantitative Pregnancy accurately.
Test 8. Apply the principles of
8. Interpretation of Tests quality assurance and
Results quality control.
9. Sources of Errors
10. QA/QC in Pregnancy
Tests
11. Manner of Reporting
Laboratory:
1. Pregnancy Tests
Biologic tests
Immunologic
Other Tests
2. Laboratory Safety
XI. Fecal Analysis At the end of the unit, the Lecture Quizzes Textbooks
14 Lecture: student is able to: Class Discussion Long exams Reference materials
1. Definition of Terms 1. Describe the normal Film Viewing Recitation Printed notes
2. Patient Preparation and abnormal color and Overhead projectors
Demonstration Practical exams
3. Specimen Collection, consistency of stool. Motivational
processing, handling, 2. Trace the formation of Group Presentation readings
preservation and disposal stool. Visual charts
4. Physiology of Stool 3. Perform the routine Laboratory manuals
Formation and special fecal Colored atlas

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 5. Routine Fecalysis analysis. Refereed journals
5.1. Macroscopic 4. Characterize specimen
5.1.1 Color acceptability.
5.1.2 Consistency 5. Correlate test results
5.2.Chemical Tests (Occult with pathologic
Blood) conditions.
5.3. Microscopic Examination 6. Discuss variables that
6. Special/Additional Fecal affect test result.
Tests 7. Apply concepts and
7. Clinical Significance principles of quality
8. Sources of Error control.
9. QA/QC in Fecal analysis
10. Manner of Reporting
Laboratory:
1. Routine Fecalysis
Macroscopic Examination
1.2. Chemical Tests
1.3. Microscopic Examination
2. Special Tests
Laboratory Safety
XII. Sputum Examination At the end of the unit, the Lecture Quizzes Textbooks
15 Lecture: student is able to: Class Discussion Long exams Reference materials
1. Definition of Terms Film Viewing Recitation Printed notes
2. Formation of sputum 1. Describe the Overhead projectors
Demonstration Practical exams
3. Patient requirements and macroscopic Motivational
preparation Group Presentation readings
characteristics and
4. Acceptability of sample Visual charts
structure of sputum.
5. Specimen collection, Laboratory manuals
transport, handling, Colored atlas
2. Trace the formation of
processing, and disposal Refereed journals
6. Routine Examination sputum
6.1. Macroscopic
3. Perform the routine
Examination
6.2. Microscopic Examination and special sputum
6.3. Other tests: analysis.
6.3.1.Gram Stain
6.3.2. Acid Fast Stain 4. Characterize specimen
7. Clinical Significance acceptability.
8. Sources of Error

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 9. QA/QC in sputum analysis 5. Correlate test results
10. Manner of Reporting with pathologic
Laboratory: conditions
1. Examination of sputum
1.1. Macroscopic 6. Discuss variables that
Examination affect test result.
1.1.1. Color
1.1.2. Consistency 7. Apply concepts and
1.1.3. Macroscopic structures
principles of quality
1.2. Microscopic
1.2.1. Gram staining control.
1.2.2. Formed Elements
1.2.3. Bacteria/Fungi
1.2.4. Cells and other
microscopic structures
1.2.5. Parasites
2. Laboratory safety
XIII. Examination of Synovial At the end of the unit, the Lecture Quizzes Textbooks
Fluid, Transudates, and student is able to: Class Discussion Long exams Reference materials
16 Exudates 1. Describe the physical Film Viewing Recitation Printed notes
Lecture: characteristics of these Practical exams Overhead projectors
Demonstration
1. Definitions of terms fluids. Motivational
2. Formation of these various 2. Trace the formation of Group Presentation readings
fluids these fluids. Visual charts
3. Patient preparation and 3. Perform the routine Laboratory manuals
requirements and special analysis on Colored atlas
4. Acceptability of samples these fluids. Refereed journals
5. Specimen collection, 4. Characterize specimen
transport, handling, acceptability.
processing, and disposal 5. Correlate test results
6. Macroscopic Examination with pathologic
7. Chemical Examination conditions.
8. Microscopic Examination 6. Discuss variables that
9. Special Test for these affect test result.
fluids 7. Apply concepts and
10. Clinical Significance principles of quality
11. Reference Range control.
12. QA and QC
13. Manner of reporting

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 14. Laboratory safety
Other Body Fluids: At the end of the unit, the Lecture Quizzes Textbooks
1. Amniotic Fluid student is able to: Class Discussion Long exams Reference materials
1.1. Definition of terms 1. Describe the physical Film Viewing Recitation Printed notes
1.2. Formation and characteristics of these Practical exams Overhead projectors
Demonstration
Composition fluids. Motivational
1.3. Patient requirements and 2. Trace the formation of Group Presentation readings
preparation these fluids. Visual charts
1.4. Acceptability of samples 3. Perform the routine Laboratory manuals
1.5. Specimen considerations and special analysis on Colored atlas
1.6. Macroscopic examination these fluids. Refereed journals
1.7. Amniotic fluid 4. Characterize specimen
examinations acceptability.
1.8. Reference values 5. Correlate test results
1.9. Clinical significance with pathologic
1.10. Sources of errors conditions
1.11. QA and QC in Amniotic 6. Discuss variables that
Fluid Analysis affect test result.
2. Gastric Juice and 7. Apply concepts and
17
Duodenal Contents principles of quality
2.1. Definition of terms control.
2.2. Formation and
Composition
2.3. Patient Requirements
and Preparation
2.4. Specimen
Considerations
2.5. Macroscopic examination
2.6. Microscopic examination
2.7. Test for Total Acidity,
Free HCl, etc.
2.8. Reference range
2.9. Sources of errors
2.10. Clinical significance
2.11. Sources of errors
2.12. QC and QA in these
fluids
FINAL EXAMINATION

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 18

Course Code & Course Plan Committee: Reviewed by: Approved by: Noted:
Course Title:
SUPACHAI A. BASIT, RMT, PhD MARIA TERESA SANTOS, MAEdLS SUPACHAI A. BASIT, RMT, PhD GEORGINA B. PALMARIO, DMD, PhD
MCC3 31
ANALYSIS OF URINE Faculty Program Coordinator Chief Librarian Dean, School of Medical Technology Vice President for Academic Affairs
AND OTHER BODY
FLUIDS

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