Agilent ChemStation

for LC Systems

Installing Your
ChemStation

A
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Installing ChemStation for LC Systems

In This Guide…
This guide describes how to install the initial software, how to add further
instrument modules, how to configure your analytical system, and how to
verify that the installation and configuration are complete and operational.

1 Installing Agilent ChemStation

This chapter provides a description of the installation process.
It gives you an overview of the complete installation procedure, starting with
the PC and operating system requirements and ending with the connection of
the analytical instruments.
The installation of the Agilent ChemStation software itself is described in
detail in this chapter.

2 Prerequisites
This chapter lists the minimum requirements for successful operation of the
Agilent ChemStation software.
It provides detailed information about the requirements that must be met to
ensure proper operation of the Agilent ChemStation. Requirements include PC
type and performance, network protocol, printer type, GPIB cards, and the
operating systems.

3 Installing Communication Components

This chapter will give you the background information that you need in order
to prepare your Agilent ChemStation hardware and operating environment.
You will have to verify that your PC meets the hardware and software
requirements for the Agilent ChemStation and do the necessary adjustments
where appropriate.
If you bought your Agilent ChemStation as a bundle from the Agilent
Technologies Chemical Analysis Group, these steps have already been
performed for you. In this case, PC hardware has already been set up for you,
the GPIB card has been installed and configured, the operating system and
operating environment have been preloaded on your computer’s hard-disk,
and the Agilent ChemStation has been installed.

Installing ChemStation for LC Systems 3

 4 Installing the Analytical Instruments
This chapter describes how to connect the HP 1050, HP 1090 and Agilent 1100
LC analytical instruments (and specific detectors) to the Agilent ChemStation
using a LAN or a GPIB connection. Default GPIB address settings and
firmware requirements for the LC Instruments are also described.

6 Configuring the Analytical System

The first section in this chapter describes how to configure the communication
of your Agilent ChemStation software with your LC instruments using a GPIB
or LAN connection with the Agilent ChemStation Configuration Editor.
The second section describes how to configure a bootp server that is required
for communication using a LAN connection.

5 Checking Out the Agilent ChemStation

This chapter describes how to perform a short system integrity test.
The steps outlined in the chapter help you when validating your system
installation.

7 Troubleshooting
This chapter will provide elementary troubleshooting information in case
something goes wrong.
The chapter will cover the most common software start-up problems, printer
problems and computer problems and how to overcome them.

8 Maintaining the Agilent ChemStation Computer

On every system, regular maintenance is mandatory to assure correct
operation.
In this chapter, we will describe maintenance steps that you should perform
on a regular basis. These steps include cleaning out left-over temporary files,
checking the integrity of the logical and physical structure of the file system,
virus-checking and regular back-ups.

4 Installing ChemStation for LC Systems

 9 Upgrading From a Previous Revision of ChemStation
This chapter describes the steps required to perform upgrades from previous
revisions of the ChemStation and to port your sequence, method and data files
to the new architecture.
Your upgrade scenario will differ according to the ChemStation revision from
which you wish to upgrade. Each scenario will be covered in a separate
section in this chapter, each section covering the upgrade from the previous
revision.

10 Supplementary Information

Installing ChemStation for LC Systems 5

6 Installing ChemStation for LC Systems
Contents
1 Installing Agilent ChemStation 15
Installation Procedure 16
Contents of the Agilent ChemStation CD-ROM 17
Agilent ChemStation Modules 17
Installation Verification Utility for Installation Qualification 17
Agilent ChemStation Software Status Bulletin (SSB) 17
Agilent ChemStation Revision History 18
I/O Libraries for the Agilent 82350 A/B GPIB Interface 18
Bootp Server 18
Agilent ChemStation Product Documentation 18
What Is Reference Information and Where Is It? 19
What Is Task-Orientated Information and Where Is It? 19
What Is the Integrated Tutorial for? 19
Where Can I Find the Description of Agilent ChemStation Commands and Data
Structures? 20
I Upgraded from a Previous Version - How Do I Know What Has Changed Since
Then? 20
Agilent ChemStation Product Structure 21
Additional Data Evaluation Modules 22
Installing the Agilent ChemStation 23
Overview 23
Installation Procedure 23
Setting Up a Shared Network-Server Based — Installation 30
What is Server-Based Installation? 30
System Requirements for a Server-Based Agilent ChemStation
Installation 31

Installing ChemStation for LC Systems 7

Contents

How to Perform a Server-Based Installation of the Agilent ChemStation 31

Installing Agilent ChemStation System Files to the Network Server 32
Activating a Shared Installation of the Agilent ChemStation for Use from a
Client PC 33
Activating the XML based interface 37
Uninstalling the ChemStation 38

2 Prerequisites 41
Agilent ChemStation PC Hardware Details 42
Minimum PC Hardware Requirements for the Agilent ChemStation 43
Non-HP Computers 43
Printers for the Agilent ChemStation 44
Advanced Power Management Not Supported with Analytical Hardware 45
Operating System Requirements 46
Number of Instruments Supported 47

3 Installing Communication Components 49

Installing LAN Communication 50
Installing TCP/IP on Windows 2000 Systems 50
Installing the CAG Bootp Server Program 52
GPIB Communication 53
Instrument Compatibility Matrix 53
Agilent 82341C GPIB Interface Card 54
Agilent 82350 A/B GPIB Interface card 55
Installing an GPIB Interface Board in the PC 56
GPIB Cabling 57
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers 58
GPIB board Configuration with the Agilent ChemStation 64

4 Installing the Analytical Instruments 65

Connecting an Agilent 1100 Series LC to the Agilent ChemStation 66

8 Installing ChemStation for LC Systems

 Contents

Instrument cabling 66
Connecting Agilent 1100 Series Modules to the Agilent ChemStation using
GPIB 67
Connecting Agilent 1100 modules to the Agilent ChemStation using LAN 70
Connecting an HP 1090 LC to the Agilent ChemStation 72
GPIB Cabling 72
Remote Cable 73
Setting the GPIB Address 76
Connecting HP 1050 Series Modules 77
Connecting Miscellaneous Instruments to the Agilent ChemStation 81
Setting the GPIB Addresses 81
Remote Control Cabling 82
LC Instrument Firmware Requirements 83

5 Checking Out the Agilent ChemStation 87

Agilent ChemStation Installation Verification 88
Overview 88
Installation Qualification 88
Adding the Agilent ChemStation Installation Verification Utility 89
Agilent ChemStation Installation Verification Utility Execution 89
Agilent ChemStation IQ Utility Results 90
Generating an Installation Verification Reference File on a Customized
Installation 93
Qualifying Operation — Agilent ChemStation Verification Test 94

6 Configuring the Analytical System 97

Using the Agilent ChemStation Configuration Editor 98
What is the Agilent ChemStation Configuration Editor? 98
Starting the Configuration Editor 99
What You Do 99
Select and Configure the GPIB Interface 100

Installing ChemStation for LC Systems 9

Contents

Configuring Paths 100

Example for LC Configurations 102
Configuring an Agilent ChemStation Instrument 102
Configuring an Agilent 1100 Series LC System 103
Configuring an HP 1050 LC System 105
Configuring an HP 1090 LC System 108
Using the Bootp Protocol 109
What is the Bootp Protocol 109
Configuring the CAG Bootp Server Program 110
Configuring the JetDirect Card Using Bootp 112

7 Troubleshooting 115
Troubleshooting LAN Communication 116
Power fail reported on the Agilent ChemStation 116
Frequent Buffer Overrun in Instrument Log Book 117
Troubleshooting GPIB Interface Problems 118
Resolving Agilent 82341 I/O Port Conflicts 119
Resolving Agilent 82341 IRQ Conflicts 120
“Autoadd Button” of IOCFG Installs Defaults Incompatible with the Agilent
ChemStation 120
Software Start-up Problems 121
An online core must be purchased in order to install additional instrument
modules 121
ChemStation cannot be located on your system. You must first install
Agilent ChemStation before installing an instrument 121
ChemStation online session does not finish start-up 121
…key in section [PCS…] of WIN.INI not valid or specified file does not
exist 123
General Protection Fault in Module... 125
Printing Problems 126
ChemStation Print Spooler hangs after an error occurred 126

10 Installing ChemStation for LC Systems

 Contents

Printing in Multi-Instrument configurations 126

Recovering from Printer hang-ups 127
Printing Messages 128
Computer Problems 130
Sporadic Hang-Ups 130
System Crash in an Online Session 130
Cannot create file… 130
Slow Hard-Disk access or Hard-Disk activity LED is continuously
flickering 130
LC Instrument Start-up Problems 132

8 Maintaining the Agilent ChemStation Computer 135

Cleaning up Left-Over Temporary Files 136
Notes on the Usage of Long File Names 136
Maintaining the PC’s File System 137
Scanning and Repairing NTFS Volumes 137
Defragmenting an NTFS Volume 137
Scanning for Viruses 138
Performing Backups 139
Windows 2000/XP Backup 139
Automating and Scheduling Windows 2000/XP Backup 139
Travan Tape Drives 140
Digital Audio Tape (DAT) Drives 140
Magneto-Optical (MO) Disk Drives 140
Recordable CD-ROM 141
Network Servers 141
Gathering System Information 142
Gathering System Information with Windows 2000/XP 142
Using the DrWatson Utility 143
Who Is Dr. Watson? 143
Dr. Watson in Windows 2000 /XP 144

Installing ChemStation for LC Systems 11

Contents

9 Upgrading From a Previous Revision of ChemStation 145

Introduction 146
Importing Files from a Previous ChemStation Revision 147
Importing Sequence Files from Revisions Prior to A.03.01 147
Importing Data Files 149
Importing Customized Report Templates 150
Delete a User Report Style 150
Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series) 151
Importing Method Files 151
Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series) 153
Importing Method Files 153
Upgrading from A.02.0x of the HPLC ChemStation (DOS Series) 154
Importing Method Files 154
Upgrading from A.03.0x of the ChemStation 155
Enhanced Integrator 155
Internal Storage Format of Quantification Data 156
Upgrading from A.03.0x Sequencing User Interface 156
Upgrading from A.03.0x methods 157
Transferring User Report Styles 158
Upgrading from A.04.0x of the ChemStation 159
A.05.0x Default Methods Use the Enhanced Integrator 159
A.05.0x Methods May Use Enhanced Peak Purity 160
Upgrading from A.05.0x of the ChemStation 161
Upgrading from A.06.0x of the ChemStation 162
Upgrading from A.07.0x of the ChemStation 163
Upgrading from A.08.0x of the ChemStation 164
Upgrading from A.09.0x of the ChemStation 165
Importing HPLC ChemStation (Pascal Series) Files 167

12 Installing ChemStation for LC Systems

 Contents

Overview 167
Importing Pascal ChemStation files using the LIF Utility 168
Before You Start 168
Converting ChemStation Data Files using the LIF Utility 170
Converting Libraries from the HPLC ChemStation (Pascal Series) to the
ChemStation using the LIF Utility 171

10 Supplementary Information 175

Agilent ChemStation Revision Code Convention 176
Agilent Technologies Response Center Support Services 177
Calling the Agilent Technologies Analytical Response Center 178

Index 179

Installing ChemStation for LC Systems 13

Contents

14 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

1
Installing Agilent ChemStation
Installation Procedure 16
Contents of the Agilent ChemStation CD-ROM 17
Agilent ChemStation Product Structure 21
Installing the Agilent ChemStation 23
Setting Up a Shared Network-Server Based — Installation 30
Uninstalling the ChemStation 38

Agilent Technologies 15
1 Installing Agilent ChemStation
Installation Procedure

Installation Procedure

To ensure the successful installation of the Agilent ChemStation and the

connection of analytical instruments, we strongly recommend to follow the
following step by step procedure.
1 Check the computer and operating systems requirements in Chapter 2,
“Prerequisites”
2 Install the Agilent ChemStation software as described in, section “Installing
the Agilent ChemStation” on page 23
3 If required install the drivers for GPIB communication or the bootp server
as described in Chapter 3, “Installing Communication Components”
4 Install the analytical instruments as described in Chapter 4, “Installing the
Analytical Instruments”
5 Configure the Agilent ChemStation software according to the chosen
installation as described in Chapter 6, “Configuring the Analytical System”
6 Examine the installation as described in Chapter 5, “Checking Out the
Agilent ChemStation”

16 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Contents of the Agilent ChemStation CD-ROM

Contents of the Agilent ChemStation CD-ROM

Agilent ChemStation Modules

The CD-ROM contains the executable files for all modules that belong to the
Agilent ChemStation product family. In order to install a module, you have to
supply a license registration number valid for the module. Those numbers
“unlock” the protected CD-ROM. They are included with the original product
and are your proof of license.
There is a main setup program setup.exe for the Agilent ChemStation modules
located in the root directory on the Agilent ChemStation CD-ROM.

Installation Verification Utility for Installation Qualification

The Agilent ChemStation comes with a utility that allows to qualify and
document the completeness and integrity of a new installation. The
Installation Verification Utility is installed automatically with the Agilent
ChemStation.

Agilent ChemStation Software Status Bulletin (SSB)

The Software Status Bulletin is a document reflecting the results of Agilent
Technologies defect logging, tracking, and repair methodology by publishing
the current status of Agilent ChemStation application software products
(known defects, available fixes, additional information).
The SSB is located in the SUPPORT\SSB directory of the Agilent ChemStation
CD-ROM.

Installing ChemStation for LC Systems 17

1 Installing Agilent ChemStation
Contents of the Agilent ChemStation CD-ROM

Agilent ChemStation Revision History

The revision history is targeted towards users who may have to consider
revalidating their analytical data system after upgrading to a new revision of
the application software. The history files are located in the SUPPORT\
HISTORY directory of the Agilent ChemStation CD-ROM.

I/O Libraries for the Agilent 82350 A/B GPIB Interface

The Agilent ChemStation CD-ROM contains the version of the I/O Libraries
that have been successfully tested with A.10.01 of the Agilent ChemStation.
The I/O Libraries must be installed separately, as described in “Configuring
the Agilent 82341 or Agilent 82350 GPIB Interface Drivers” on page 58.

Bootp Server
The Agilent ChemStation CD-ROM contains a bootp server setup in the
directory bootp that can be used to supply the analytical instruments that are
connected to a LAN with an IP address and configuration settings. This bootp
server is easy to configure for the use of analytical instruments using a LAN
connection. Refer to section “What is the Bootp Protocol” on page 109 for
more information about the bootp server.

Agilent ChemStation Product Documentation

The Agilent ChemStation product documentation consists of paper handbooks
with reference information and online documentation for task-orientated
topics. The paper manuals are also located on the CD-ROM in the directory
manuals, together with the required Adobe Acrobat reader.
The newly implemented XML interface is fully documented in the Agilent
ChemStation XML Interface Guide, which is available as a PDF document on
the ChemStation CD-ROM.

18 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Contents of the Agilent ChemStation CD-ROM

What Is Reference Information and Where Is It?

With the documentation strategy for the Agilent ChemStation chosen by
Agilent Technologies, all reference information, i.e. explanation of general
concepts, algorithms and formulae is contained in the “Understanding”
handbooks. The reference handbooks generally do not provide any
task-orientated information (e.g. “How to set up a sequence”).
The Agilent ChemStation handbooks do not cover details on analytical
hardware (other than those required for installation and configuration for
operation with the Agilent ChemStation).

What Is Task-Orientated Information and Where Is It?

Task-orientated information describes how to perform a certain task and is
therefore very context-specific. The context-specific “How-To” information is
contained in the Agilent ChemStation help-system. The help-system always
can be evoked by pressing the F1 function key. the Agilent ChemStation
“Help”-menu, or the “Help” button available in Agilent ChemStation dialog
boxes.
Access the “Help/Help Topics” menu and click the “LC Tasks” or “GC Tasks”
index. Select the analytical task that you need instructions for.

What Is the Integrated Tutorial for?

Your Agilent ChemStation comes with an integrated tutorial. “Integrated”
means: Rather than simulating it, the tutorial lets you work with the live
Agilent ChemStation. If you cannot find the menu or button to accomplish a
certain task, use the “Show Me” button from the task’s help screen to have the
tutorial show you what to do by pointing the mouse cursor to the appropriate
spot on the screen and pressing the appropriate menu or button for you.

Installing ChemStation for LC Systems 19

1 Installing Agilent ChemStation
Contents of the Agilent ChemStation CD-ROM

The tutorial does not limit you to a predetermined scenario. For instance, you
can use the “Setting Up an Analysis” task to define your first Agilent
ChemStation method.
You start the Agilent ChemStation tutorial from the “Help/Tutorial” menu. The
tasks displayed in the tutorial main screen depend on your current context,
e.g. if your current Agilent ChemStation view is “Method and Run Control”,
the tutorial main screen only displays tasks available in this view.

Where Can I Find the Description of Agilent ChemStation

Commands and Data Structures?
The Macro Programming Guide and Commands Reference are available for
advanced users and Agilent ChemStation application developers. The
Commands reference information can be accessed from the
Agilent ChemStation help menu (“Help/Commands”) and is structured into
command categories and the command descriptions with syntax, parameters,
return values along with a discussion and frequent examples.
The Macro Programming Guide is available electronically through the Agilent
ChemStation help system.

I Upgraded from a Previous Version - How Do I Know What Has

Changed Since Then?
When starting the Agilent ChemStation for the first time, you can select to go
to the What’s new section of the help. Later on you can always go to the table
of content of the help file and select new features to find out what feature have
been added to the Agilent ChemStation.
A list of all defect fixes since the last revision is located in the directory
support\history.

20 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Agilent ChemStation Product Structure

Agilent ChemStation Product Structure

The Agilent ChemStation for GC, LC, LC/MS, CE and A/D has been designed
as a series of software modules for instrument control, data acquisition, data
analysis and reporting. Technique-specific single instrument modules provide
control capabilities for a given separation technique, e.g. for one Gas or Liquid
Chromatograph. A single instrument configuration may be expanded by
adding an additional instrument control module. With this approach, one
Agilent ChemStation is capable of controlling more than one analytical
system, e.g. two chromatographs of the same or different separation
techniques.
Please note the memory requirements for every additional instrument module
(see “Minimum PC Hardware Requirements for the Agilent ChemStation” on
page 43).

Data Analysis Modules ChemStore C/S Spectral Evaluation Solution

(optional) Database Module Module (3D) Add Ons

Chromatography Core Module 2D Chromatography Core

(generic)
Instrument Control Modules A/D CE GC LC LC-MSD
(optional, technique-specific)

Figure 1 Agilent ChemStation Module Architecture

For further information regarding Solution Add-Ons (e.g. Purify Software,

ChemAccess) please contact your local administration.

Installing ChemStation for LC Systems 21

1 Installing Agilent ChemStation
Agilent ChemStation Product Structure

Additional Data Evaluation Modules

The acquired data is typically two-dimensional (“2D”), i.e. by measuring
detector response over time. Spectroscopic detectors can deliver
three-dimensional (“3D”) data, by additionally measuring detector response
over a third axis (e.g. wavelength or mass range). The optional “Spectral
Evaluation Module” permits analysis and reporting of this “3D” data and is
included with the CE and LC/MS modules, and optional for an LC module.
The Agilent ChemStore Database Module, a results storage and organization
module may be added to all separation techniques.
The Agilent ChemStation is capable of controlling up to four “2D” instruments
or up to two “3D” instruments concurrently. For LC/MS and CE, CE/MS no
additional instruments are supported on the PC system.
Please note the memory requirements for each additional data evaluation
module For further details on supported configurations and their PC
requirements, see“Minimum PC Hardware Requirements for the
Agilent ChemStation” on page 43.

G2180AA (spectra evaluation) ChemStore C/S Spectral Evaluation

and Database Module Module (3D)

G2170AA 2D Chromatography Core

(Agilent ChemStation for LC)
A/D CE GC LC LC-MSD

Figure 2 A combination of modules results in an Agilent ChemStation for a specific

technique. The example illustrates a typical configuration for LC. The driver for
one LC instrument is included in the G2170AA Instrument Core Module.

22 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Installing the Agilent ChemStation

Installing the Agilent ChemStation

Overview
This section explains how to use the enclosed CD-ROM to:
• install the Agilent ChemStation for the first time
• upgrade existing Agilent ChemStation software
• install an additional Agilent ChemStation module

Installation Procedure
Use the following procedure if you are using the CD-ROM to install or upgrade
the Agilent ChemStation.
Before installing the software:
• Set up the computer hardware:
If you are going to connect your instruments using a GPIB connection,
install an GPIB communication board in your PC as described in “Installing
an GPIB Interface Board in the PC” on page 56.
• Set up the computer settings:
It is recommended that the Regional options in the Control Panel of your
System are set to English (US). If another location is used, the following
settings are mandatory:
• Decimal symbol = . (point)
• Digit grouping symbol = , (comma)
• List seperator = , (comma)
1 Install the Windows 2000 Professional or Windows XP Professional
operating system on the PC. Refer to the documentation delivered with
your PC or with the operating system. Start the operating system. Please
make sure that your logon does have administrative capabilities, as changes
will be made to the win.ini and the Windows registry.
2 Check the partitioning scheme of your PC and ensure that the Agilent
ChemStation is installed on a large enough partition and that the path to

Installing ChemStation for LC Systems 23

1 Installing Agilent ChemStation
Installing the Agilent ChemStation

the data directories is configured to a large enough partition. (See

“Configuring Paths” on page 100.
3 Insert Agilent ChemStation CD-ROM into the CD-ROM drive.
4 From the Start menu in the Task Bar, select Start > Run.
5 At the command line, type diskdrive:\Setup (e.g., D:\Setup), then click
on OK.
If this is an installation on a new computer, proceed with step 5.

If you are upgrading your system or adding an additional module, click on

Yes to have the Setup program update all existing Agilent ChemStation
software on your PC. This guarantees all Agilent ChemStation instrument
modules have the same version. Click on No to exit the program without
installing anything.
After updating the existing Agilent ChemStation installation, you can
specify whether to install additional modules such as additional instrument
control or add-on software modules. If no, you may skip the following steps
and start working with the new version of the Agilent ChemStation.

6 If you need to install an additional module, specify the number of the

instrument that you want to add or modify.

24 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Installing the Agilent ChemStation

Installing ChemStation for LC Systems 25

1 Installing Agilent ChemStation
Installing the Agilent ChemStation

Select an instrument number and click on “Add/Change”.

7 Select the appropriate software module from the list of available products
in the “Configure Instrument” dialog and press “Add”.
8 Enter the License Registration Number for the selected module in the “New
License Number” Field and press “Add”.
The applicable product and license numbers are printed on your Software
Certificate and Registration Packet (see the example license registration
label).

26 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Installing the Agilent ChemStation

SOFTWARE REGISTRATION LABEL FOR

PRODUCT NUMBER

LE
G2170AA

MP REVISION CODE
A.10.01

SA REGISTRATION
NUMBER
DL08E8888A
Figure 3 Example License Registration Label

If you need to install another module for this instrument, e.g. an additional
data evaluation module, proceed with step 5. If you have purchased more
than one instrument control module license for this PC, click OK and
proceed with step 4 to install it as an additional instrument.
9 Check that the path points to the correct partition, default is C:\. Click on
Install of the Instrument Setup box to continue.
10 Click on Yes to allow the Setup program update the path statement in your
AUTOEXEC.BAT file. Choose No only if you are upgrading your
Agilent ChemStation installation and you have not changed your
installation directory.

Although Windows 2000 and Windows XP Professional ignore most entries in the
NOTE AUTOEXEC.BAT file, the path entry is still used and will modify the system environment
variable PATH used by the operating system.

Installing ChemStation for LC Systems 27

1 Installing Agilent ChemStation
Installing the Agilent ChemStation

If you select No, don’t forget to add the Path statement to your autoexec.bat file
CAUTION
manually, later. the Agilent ChemStation directory (default: C:\HPCHEM) and
Agilent ChemStation system directory (default: C:\HPCHEM\SYS) must be included in
your PATH setting.

11 The Setup program will prompt you to configure any new instruments you
installed.

Click on Yes to access the Agilent Configuration Editor and configure your
instruments. Click on No to exit the Setup program and return to Windows.
12 Your software installation is now complete. The Setup program made
changes to your WIN.INI file and saved a copy of the original WIN.INI file in
the files named WIN.XXX (i.e., WIN.000, WIN.001, WIN.002, WIN.003) in the
directory “C:\HPCHEM\BACKUP”.
Remove and store your CD-ROM in a safe place. See “Installing the
Analytical Instruments” on page 65 for details of how to install your
analytical instruments.

28 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Installing the Agilent ChemStation

Save your license numbers in a safe place. These will be required if you have
to reinstall your software or for future upgrades.
13 After you install and configure the Agilent ChemStation software, be sure
to exit Windows and reboot your system before starting the ChemStation so
the changes in your AUTOEXEC.BAT file go into effect.

Installing ChemStation for LC Systems 29

1 Installing Agilent ChemStation
Setting Up a Shared Network-Server Based — Installation

Setting Up a Shared Network-Server Based — Installation

What is Server-Based Installation?

For the convenience of Agilent ChemStation system administrators who
manage multiple analytical computers in a networked laboratory
environment, the ChemStation software revision A.04.01 (and greater) can be
installed to and run from a shared network drive. The network server holds
the Agilent ChemStation system software, allowing the appropriate number of
licensed Agilent ChemStation clients to execute the same Agilent ChemStation
code locally. The analytical instruments are connected to the client systems.
Instrument control, data acquisition and data evaluation are performed by the
clients.
The shared network-server based installation of the Agilent ChemStation
allows you to administer one installation of the Agilent ChemStation software,
requiring you to maintain and validate the system software only once.

30 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Setting Up a Shared Network-Server Based — Installation

System Requirements for a Server-Based Agilent ChemStation

Installation
• Each client PC must meet the hardware and software requirements for the
Agilent ChemStation.
• The PC used to install the Agilent ChemStation system software to the
shared drive on the network server must be equipped with a CD-ROM drive
• The networking components installed on the client PCs must be compatible
with the networking support built into Microsoft Windows.
• The client PCs must have loaded the networking components and logged on
to the network server successfully.
• The client PCs must have access to the appropriate shared drives on the
network server.
• The network server should be a dedicated server machine (e.g. HP
Netserver) running a server operating system (e.g. Windows 2000 Server).
The network protocol used should be routable (e.g. TCP/IP).

How to Perform a Server-Based Installation of the

Agilent ChemStation
The server based Agilent ChemStation installation is a two-step process:
1 Install the Agilent ChemStation system software to a shared drive of the
network server from one of the client systems.
2 Install and configure the Agilent ChemStation instrument configuration
files on the client PCs.

Installing ChemStation for LC Systems 31

1 Installing Agilent ChemStation
Setting Up a Shared Network-Server Based — Installation

Installing Agilent ChemStation System Files to the Network Server

The steps outlined below install the Agilent ChemStation system files to a shared
drive on the network server. You are not required to install the Agilent ChemStation
system files using the network operating system of the server.
1 Start Windows on the client PC that you wish to use to install the
Agilent ChemStation system files to the network server.
2 Map a drive letter to the share on the server from each client PC. This drive
mapping should be permanent.
3 Insert the Agilent ChemStation Installation CD-ROM.
4 Execute the server installation program (e.g. D:\SERVER\SETUP.EXE,
where d: is the drive identifier of the CD-ROM drive).
5 In the Installation Location dialog, specify the drive and directory that will
contain the Agilent ChemStation system files on the shared network drive.

S:\HPCHEM, where S: is the drive identifier of the shared network drive.

6 The server installation procedure now copies all Agilent ChemStation system
files to the destination specified in the installation location dialog box.

32 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Setting Up a Shared Network-Server Based — Installation

Activating a Shared Installation of the Agilent ChemStation for Use

from a Client PC
After successfully installing all Agilent ChemStation system files to a shared
network drive, the corresponding Agilent ChemStation client PCs must be
configured. This task must be performed on every client PC that is licensed to
run the shared Agilent ChemStation system files.
1 Start the Agilent ChemStation client setup program from each client by
executing S:\HPCHEM\CLIENT\SETUP.EXE, where S: is the drive
identifier of the shared network drive and HPCHEM is the directory that
contains the shared Agilent ChemStation system files.

Path specifications according to the Universal Naming Convention (UNC) are not supported
NOTE by the Agilent ChemStation installation routine. A drive identifier must be specified, which
means the network connection must be established by mapping a network drive.

2 In the Local Setup dialog box, specify the drive and directory that will
contain the local client’s instrument configuration information (default: C:\
HPCHEM).

Installing ChemStation for LC Systems 33

1 Installing Agilent ChemStation
Setting Up a Shared Network-Server Based — Installation

Table 1 Instrument Types

Use Instrument Type For These Instruments

GC HP 5890, Agilent 6890 and Agilent 6850 gas chromatographs and autosamplers

LC HP 1050, HP 1090 and Agilent 1100 LC systems and HP 1040, HP 1046A, HP 1047A and HP 1049A
special detectors

LC 3D HP 1050, HP 1090 and Agilent 1100 LC systems with diode array detectors or an HP 1040
stand-alone diode array detector (requires an additional G2180AA or G2185AA DAD spectral
evaluation module license number)

CE Agilent capillary electrophoresis instrument

A/D – GC type 35900E dual channel A/D interface systems configured gas chromatographs with two injectors
capable of simultaneous injections

A/D – LC type 35900E dual channel A/D interfaces

34 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Setting Up a Shared Network-Server Based — Installation

3 On each client, you can configure up to four 2D instrument sessions,

provided you have the appropriate software licenses and license
registration numbers.
The example given above installs a total of four instrument sessions, two LC
2D sessions, one A/D session and one GC session.

If you plan to install a multi-instrument configuration, please check the tables in the
NOTE Agilent ChemStation hardware details section in this handbook on the required PC
hardware and supported multi-instrument configurations.

4 The directories that contain the Agilent ChemStation system software must
be included in the path setting of the client PC. The Agilent ChemStation
installation procedure can make the necessary adjustments for you.
You may decide to make these adjustments yourself. If you want to modify
your system’s autoexec.bat yourself, select No, when prompted:

Installing ChemStation for LC Systems 35

1 Installing Agilent ChemStation
Setting Up a Shared Network-Server Based — Installation

5 Remember to view the Agilent ChemStation’s README.TXT file after the

installation has completed. This file contains important last-minute
information on the software that could not be included in the printed
documentation.

6 Each installed Agilent ChemStation instrument session requires a valid

license registration number, otherwise you will not be able to control the
corresponding instrument online. The license registration numbers are
attached to the Software Certificate and Registration Packet envelope
included with the delivered Agilent ChemStation software (see Figure 3 on
page 27).

7 In order to activate the changes made to your system initialization files, you
need to exit all applications and restart your computer.
8 Use the Agilent ChemStation Configuration Editor to set up your
instrument sessions (see Chapter 6, “Configuring the Analytical System”).

36 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Setting Up a Shared Network-Server Based — Installation

Activating the XML based interface

In order to activate XML support, you need to make changes to the WIN.INI
file. For full details see the Agilent ChemStation XML Interface Guide on the
ChemStation CD-ROM.

Installing ChemStation for LC Systems 37

1 Installing Agilent ChemStation
Uninstalling the ChemStation

Uninstalling the ChemStation

In some cases you might need to uninstall the ChemStation, for example, if you
wish to install it in a different location. To completely remove a ChemStation
installation please proceed with the following steps.

If any other products belonging to the ChemStation Plus Family are installed, which require
NOTE to be uninstalled using the standard Windows uninstall procedure (Control Panel >
Add/Remove programs) such as ChemStore or ChemAccess, please uninstall these
products using the Windows routine prior to deleting the HPCHEM folder and modifying
Win.ini. Please refer to the corresponding software documentation of the ChemStation Plus
product for details.

1 If the ChemStation is running close all sessions and reboot the computer.
2 Backup your ChemStation data, methods and sequences and if necessary
any customized macros such as user.mac. To do this store the following
directories on your backup device or in a save location:
• \HPCHEM\1\ contains all data, methods and sequences for instrument 1
• \HPCHEM\2\ contains all data, methods and sequences for instrument 2
• \HPCHEM\3\ contains all data, methods and sequences for instrument 3
• \HPCHEM\4\ contains all data, methods and sequences for instrument 4
• \HPCHEM\REPSTYLE contains your customized reports
• \HPCHEM\SPECLIBS contains your spectral libraries
• \HPCHEM\CHEMSTOR contains local ChemStore databases
3 Remove the complete HPCHEM folder from your hard disk.

38 Installing ChemStation for LC Systems

 Installing Agilent ChemStation 1
Uninstalling the ChemStation

4 Select Start > Run > and type Sysedit. Select OK and choose Win.ini.
Locate the general [PCS] section and the instrument specific [PCS,x]
sections where x represents the instrument number(s) ranging from 1 to 4.
Remove these sections completely. Choose Save from the File menu.

Be careful when editing the win.ini. Do not remove any other sections apart from the ones
NOTE mentioned above.

A backup copy of Win.ini is automatically stored as win.syd in your local windows directory.
NOTE If you need to restore the original Win.ini because you suspect any startup problems
originating from your change rename the current Win.ini to, for example, Win.old and
Win.syd to Win.ini.

Installing ChemStation for LC Systems 39

1 Installing Agilent ChemStation
Uninstalling the ChemStation

40 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

2
Prerequisites
Agilent ChemStation PC Hardware Details 42
Operating System Requirements 46
Number of Instruments Supported 47

Agilent Technologies 41
2 Prerequisites
Agilent ChemStation PC Hardware Details

Agilent ChemStation PC Hardware Details

This section states the PC hardware and operating system requirements that
must be met for successful installation and operation of the
Agilent ChemStation.
The Agilent ChemStation A.10.0x and greater is supported on personal
computers with an Intel Pentium III 600 MHz (or higher). Depending on the
type of GPIB interface one ISA or PCI slot is required. For details about GPIB
interface requirements refer to the section “GPIB Communication” on page 53.
If a LAN connection is used for the analytical equipment, a LAN interface
supported by the operating system is required and the Microsoft TCP/IP
protocol must be installed. All PC hardware and peripherals must be listed in
the Microsoft Hardware Compatibility List (HCL) which is available from the
Microsoft home page on the world wide web (http://www.microsoft.com). If
your PC hardware is not listed in the HCL, the system may not work correctly
with the Agilent ChemStation software.

42 Installing ChemStation for LC Systems

 Prerequisites 2
Agilent ChemStation PC Hardware Details

Minimum PC Hardware Requirements for the Agilent ChemStation

Table 2 Minimum PC Hardware Requirements for Windows 2000 / XP-Based Systems

Processor Pentium III 600 MHz

Screen resolution Super VGA (800 × 600)

Mass storage 4 GB Hard-Disk and CD-ROM

Minimum Memory for Single Instrument (2D) 128 MB of RAM

Minimum Memory for Single Instrument (3D) 256 MB of RAM

Minimum Memory for Multi Instruments 256 MB of RAM

Single instrument configuration with Agilent ChemStore 256 MB of RAM

C/S database module (rev. B.02.0x),

Multi instrument configuration with Agilent ChemStore 256 MB of RAM

C/S database module (rev. B.02.0x),

Generally a maximum of four instrument systems may be configured to each Agilent

NOTE ChemStation, but there is a limitation on the number of individual modules connected to
the Agilent ChemStation PC. Refer to “Number of Instruments Supported” on page 47

Non-HP Computers
The Agilent ChemStation has been designed to successfully run on a wide
range of compatible personal computers equipped with accessories and
peripherals that adhere to the programming standards for the Intel PC
platform and Microsoft Windows operating systems.
However, Agilent Technologies has tested the Agilent ChemStation software
mainly on Agilent Technologies equipment. All configuration information
listed in this manual applies to Hewlett-Packard/Compaq Kayak, Vectra and
EVO computers and may not be optimized for other vendor’s PCs. The
standard configuration of the GPIB interface, for instance, may conflict with
the memory configuration of a non-HP computer.
Additional accessory interface boards may cause conflicts of hardware related
resources (I/O ports, interrupt settings, DMA channels).

Installing ChemStation for LC Systems 43

2 Prerequisites
Agilent ChemStation PC Hardware Details

For a non-Hewlett-Packard computer, use the setup utility program supplied

by the manufacturer to configure your computer and check the documentation
supplied with it and the accessories to eliminate resource conflicts in your
PC’s setup, especially regarding the configuration of the GPIB interface. In
case of hardware conflicts, see “Resolving Agilent 82341 I/O Port Conflicts” on
page 119.

Printers for the Agilent ChemStation

The Agilent ChemStation has been designed to work with printers that are
compatible with the operating system. Printers may be attached to a local
(preferably parallel) or networked port on the PC. Serial port printers are
supported by the operating system but may exhibit speed performance
limitations. Networked printers must be shared by a network server running a
network protocol supported by the Microsoft operating system.
We recommend printers that are capable of interpreting an escape code
language (e.g. PCL) or page description language (e.g. PostScript). Host-based
printers (e.g. GDI or PPA printers) impose more printer processing tasks on
the CPU and are not recommended for use with Agilent ChemStation on-line
sessions.
For best printing results with your Agilent ChemStation, use HP LaserJet
printers. High performance HP DeskJet printers may also be used if the
amount of printing required is low. Check the readme.txt files for information
on recommended printer driver versions.
Agilent Technologies has not tested all printer and printer driver
combinations that are supported in the Windows environment. Print
performance and results may vary on other manufacturer’s printers and
appropriate drivers.
The printers listed in Table 3 on page 45 have been successfully tested at the
time this handbook was printed.

44 Installing ChemStation for LC Systems

 Prerequisites 2
Agilent ChemStation PC Hardware Details

Table 3 Printer Successfully Tested with Agilent ChemStation A.10.01

Printer Model Driver comment

HP Laserjet 2100 HP PCL 6 Driver

HP LaserJet 4050 HP PCL 5e Driver

HP LaserJet 4200 HP PCL 5c Driver

HP Business InkJet 2250c HP PCL

Please note that this list is not comprehensive and does not include printers and printer
NOTE drivers made available after the release of this handbook. If your printer is not listed here, it
does not mean it does not work with the Agilent ChemStation, but it was not tested.

We recommend using Hewlett-Packard LaserJet. Due to their limited print-performance (<

NOTE 8 pages per minute) and paper tray capacity (less than 150 pages), some low-end printers
may not be suitable for unattended high data-throughput operation of the Agilent
ChemStation, e. g. in multi-instrument configurations or when running sequences with
many injections and relatively short run-times (e.g less than 5 minutes run-time), even if
listed in Table 3.

Advanced Power Management Not Supported with Analytical

Hardware
The BIOS and operating systems of many modern PCs supports Advanced
Power Management (APM). After a specified idle time, the BIOS will switch
the system to stand-by by turning off the computer display, hard disk and
other devices, reducing the output of the PC’s power supply and the internal
clock frequency to save energy.
The reduction in internal clock speed and hard disk slow down can render the
PC incapable of handling the real-time requirements of instrument control and
data acquisition. Typically, this results in overflows of the internal instrument
buffers, or, (in other words) data loss. APM is not supported in systems used
for on-line operation of analytical hardware.

Installing ChemStation for LC Systems 45

2 Prerequisites
Operating System Requirements

Operating System Requirements

You will need the Windows 2000 Professional (Service Pack 2 or greater) or
Microsoft Windows XP Professional (Service Pack 1a) operating system. For
up to date information on supported operating systems please contact your
local service and support center.

Windows 95 or Windows 98, Windows ME and Windows NT are not supported with this
NOTE version of the ChemStation.

If you use a LAN to connect to the analytical instruments (Agilent 1100 only),
the Microsoft TCP/IP protocol must be installed and configured.
Window 2000 Professional or Windows XP Professional are required for the
Agilent ChemStation data acquisition and analysis software. The Microsoft
Server operating systems (NT Server and Windows 2000 Server) are not
supported for ChemStations except as application servers when using the
Agilent ChemStation in network-server based installation (see “How to
Perform a Server-Based Installation of the Agilent ChemStation” on page 31).

The HP 82335 card is not supported. The Agilent 82341C is supported on Windows 2000
NOTE only and the Agilent 82350 A/B cards are supported on Windows 2000 and Windows XP
Professional, for all analytical hardware except the instruments not designed for GPIB
communication. See Table 7 on page 53 for details.

46 Installing ChemStation for LC Systems

 Prerequisites 2
Number of Instruments Supported

Number of Instruments Supported

A maximum number of 4 instruments can be configured on a single

ChemStation PC. Each instrument can consist of several modules, like Agilent
1100 LC modules, HP 1090 mainframe with DAD, HP 1050 modules, etc. The
maximum number of instruments is reduced according to Table 4.

Table 4 Maximum Number of Instruments

Description Number of instruments

ChemStore C/S database 3

2 modules that can acquire on-line spectra, like DAD or FLD 2

Agilent LC/MSD module 1

Agilent CE module G1600A 1

There is also a limitation on the total number of analytical modules connected

to the Agilent ChemStation. Generally a maximum number of approximately
12 modules can be connected, depending on the exact configuration. The
following modules count as 2 modules: Agilent 1100 DAD, Agilent 1100 FLD,
Agilent 1100 VWD and the Agilent 6890 GC. Table 5 gives you an example for a
possible configuration, where it would not be possible to add another detector.

Table 5 Maximum Number of Modules

Agilent 1100 system A Agilent 1100 system B

Binary pump Binary pump

Variable wavelength detector Diode array detector

Autosampler Thermostatted autosampler

Thermostatted column compartment Thermostatted column compartment

Please contact your Agilent representative, to check if your exact configuration is possible,
NOTE especially when you configuration is coming close to 12 modules.

Installing ChemStation for LC Systems 47

2 Prerequisites
Number of Instruments Supported

48 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

3
Installing Communication Components
Installing LAN Communication 50
GPIB Communication 53
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers 58

Agilent Technologies 49
3 Installing Communication Components
Installing LAN Communication

Installing LAN Communication

If you decide to connect your instrument using a standard LAN connection,

you must ensure proper communication between the PC and the analytical
instruments. The communication uses the TCP/IP protocol, which needs to be
installed as a network protocol on your PC. For the configuration of the
JetDirect card that is used to connect the analytical instrument to the LAN,
the boot strap protocol is used, which requires a bootp server.
This chapter outlines the installation of the TCP/IP protocol on Windows 2000
and Windows XP systems. Windows 2000 and Windows XP systems will
typically come with TCP/IP pre-installed. Instructions are also included for
the CAG bootp server program required to configure TCP/IP parameters for
your instrument.

Installing TCP/IP on Windows 2000 Systems

1 From the Start menu in the Task Bar, select Start -> Settings -> Control
Panel.
2 Open Network and Dial-up Connections, select Local Area Connection, and
use right-mouse click to access the properties.
3 In the General tab select the Internet Protocol TCP/IP and choose
Properties.
4 Select Use the following IP address, to ensure the system does not use
DHCP address (see “DHCP Server” on page 117 for details).
5 Ask your IT department for a valid IP address, gateway, subnet mask, DNS
and WINS server, if your PC is connected to a site network. If you are
setting up your own internal network and are not connected to any other
network, you may use the example settings from Table 6. Gateway, DNS and
WINS IP addresses do not need to be configured in this case, as you do not
connect to any other part of a network. Figure 4 shows an example of the
TCP/IP configuration on Windows 2000.

50 Installing ChemStation for LC Systems

 Installing Communication Components 3
Installing LAN Communication

Table 6 Example IP addresses

PC number IP address Subnet mask

1 10.1.1.100 255.255.255.0

2 10.1.1.101 255.255.255.0

3 10.1.1.102 255.255.255.0

... .... 255.255.255.0

Figure 4 TCP/IP Configuration Screen

6 Select OK to finish the configuration.

Installing ChemStation for LC Systems 51

3 Installing Communication Components
Installing LAN Communication

Installing the CAG Bootp Server Program

If there is already a bootp server running in your network, contact your
system administrator with the information supplied in “Using the Bootp
Protocol” on page 109. If you wish to use a local bootp server, proceed as
outlined below, to install the CAG bootp server program.
1 From the Start menu in the Task Bar, select Start / Run.
2 At the command line, type diskdrive:\bootp\BootpInstall (e.g., D:\
bootp\BootpInstall), then click on OK.
3 Follow the instruction on the screen for the installation of the CAG bootp
server program. It is recommended that you read the readme.txt file, that
might contain last minute information not included in this manual.
4 The CAG bootp server program can now be started using the Bootp icon
from the Agilent ChemStations group in the start menu.

Please note that the CAG bootp server program always starts as an icon in the taskbar (as if
NOTE you hit the minimize button of the program window).

52 Installing ChemStation for LC Systems

 Installing Communication Components 3
GPIB Communication

GPIB Communication

Analytical instruments that communicate with the Agilent ChemStation via

GPIB require a GPIB board to be installed in your computer.
You can either use an Agilent 82341C (16-bit) high-performance GPIB
interface card, which is an ISAcompatible accessory card or an Agilent 82350
A/B PCI high-performance GPIB interface card.

Instrument Compatibility Matrix

The following table helps you to select the correct GPIB interface for your
system.

The HP 82335 model of the GPIB interface is not supported by Agilent ChemStations,
NOTE revision A.07.01 and higher.

Table 7 GPIB and Analytical Hardware Compatibility Matrix

Instrument Type Agilent 82341C Agilent 82350

HP 1090 / HP 1040 LC Yes Yes

HP 1050 LC Yes Yes

Agilent 1100 LC Yes Yes

HP 5890 GC Yes Yes

Agilent 6890 GC Yes Yes

Agilent 6850 GC No No

Agilent G1600A (CE) Yes Yes

Installing ChemStation for LC Systems 53

3 Installing Communication Components
GPIB Communication

The GPIB board is static sensitive and should be handled with care so as not to
CAUTION
damage it. Be sure to hold the board by the edges and do not touch the electrical
components. Use anti-static equipment, such as a grounding mat and wrist-straps,
during installation.

Agilent 82341C GPIB Interface Card

The Agilent 82341C is an ISA- compatible GPIB interface card. It uses an I/O
address range and an interrupt (IRQ) line.

Figure 5 DIP-Switch Settings of the Agilent 82341 GPIB Interface

The Agilent 82341 I/O base address is selected using switches 1 through 4 of
the DIP switch, see Figure 5. To change the base address, power down the
computer, remove the GPIB interface card, re-set the DIP switches, re-insert
the card, and power up your computer. Run I/O Config again to configure the
GPIB interface. In most cases, you will not need to change the default switch
setting. However, if the default setting conflicts with other interfaces you have
in your computer, use Table 8 on page 55 to select the desired address range to
resolve the conflict.

54 Installing ChemStation for LC Systems

 Installing Communication Components 3
GPIB Communication

Table 8 Agilent 82341C Switch Settings

Switches I/O Base Address I/O Address Range Used

1 2 3 4 (Hexadecimal) (Hexadecimal)

0 0 0 0 250 (default) 250–257

1 0 0 0 270 270–277

0 1 0 0 350 350–357

1 1 0 0 370 370–377

0 0 1 0 220 220–227

1 0 1 0 280 280–87

0 1 1 0 390 390–397

1 1 1 0 380 380–387

The selected I/O address ranges must not conflict with other I/O interfaces installed in
NOTE your computer, including products of other manufacturers (for example LAN interfaces).
Refer to the documentation for the other interfaces and Table 8 to select unique addresses
for all the interfaces in your computer.

Because the Agilent 82341C interface is I/O port-mapped and not memory-mapped, it is not
NOTE necessary to exclude address ranges for memory management software on your computer.

Agilent 82350 A/B GPIB Interface card

The Agilent 82350 is a PCI GPIB interface card. Therefore no additional
settings such as changing the I/O base address are required.

Installing ChemStation for LC Systems 55

3 Installing Communication Components
GPIB Communication

Installing an GPIB Interface Board in the PC

Since these boards can be installed in several different computers, the
following instructions are general. If you have difficulties during the
installation, consult your computer manual or dealer.

Turn off and unplug your computer and all attached electrical devices before
WA R N I N G
removing any covers.

1 Turn off and unplug your computer, then remove the computer’s cover(s).
2 Select any empty ISAcompatible or PCI slot depending on the type of GPIB
board. It is best not to place the GPIB board next to a graphics adapter. As
the GPIB connector is wider than a typical PC slot, you may have to leave
the slot(s) adjacent to the GPIB interface empty.
3 If you have more than one GPIB board in your computer, or if some other
system setting conflicts with the resources associated with the default
factory setting for the GPIB board, be sure to reset the switch settings to
avoid resource conflicts as described above (only applicable for the Agilent
82341C card).
4 Write down the slot number you will use and store it for later use. Some
EISA computers require slot number and board type information after
installation. For computers that require this information, refer to the
appropriate installation documentation.
5 Loosen the mounting screw and remove the selected empty slot’s rear plate.
6 Holding the board by its edges, insert the board into its slot. Make certain
that the board’s edge connector is fully seated. Lock the board into place
with the mounting screw.
7 Replace the computer's cover(s). Plug in, and restart the computer.

56 Installing ChemStation for LC Systems

 Installing Communication Components 3
GPIB Communication

GPIB Cabling
When connecting GPIB devices together, there are several basic rules that
should be observed.
1 Whenever possible, turn off and unplug the computer and all attached
devices before the GPIB cables are installed.
2 Before connecting any analytical instrument to an GPIB cable, consult the
documentation supplied with each device and determine its GPIB address.
No two devices connected to the Agilent ChemStation may have the same
address. Alter their addresses as necessary to avoid any duplication. Write
down each GPIB address. This information will be needed later.
3 Try to use short GPIB cables that are two meters long or less.
GPIB cable (0.5 m) (10833D)
GPIB cable (1.0 m) (10833A)
GPIB cable (2.0 m) (10833B)
GPIB cable (4.0 m) (10833C)
4 Connect one end of an GPIB cable to the computer’s GPIB connector.
5 Be certain that you properly tighten all GPIB connectors. A poor connection
causes errors which are difficult to diagnose.
6 Connect GPIB devices in a chain. A chain occurs when an GPIB device is
connected to the next GPIB device, and it is, in turn, connected to the next,
and so on. Avoid star configurations (connecting all of the devices to a
central point).

According to the IEEE 488 specification for the General Purpose Instrument Bus
WA R N I N G
(GPIB), the bus is not designed for dynamic configuration. Cycling power on an GPIB
instrument that is connected to the bus while other GPIB instruments are actively
communicating to the GPIB controller can induce electrical spikes that may
potentially corrupt the GPIB protocol. In extreme cases. this may require cycling
power on all instruments, including the GPIB controller (i.e. typically the Agilent
ChemStation).

Installing ChemStation for LC Systems 57

3 Installing Communication Components
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

Configuring the Agilent 82341 or Agilent 82350 GPIB Interface

Drivers

After installing the GPIB board in the computer, it is necessary to install the
corresponding driver and configuration software, located on the Agilent
ChemStation CD-ROM in the “\GPIB” directory. An IO Libs installing guide
can be found on the CD-ROM as well. To install the I/O Libraries proceed with
the following steps:

The Agilent ChemStation A.10.01 and greater requires version L.02.00.01 of the I/O
NOTE Libraries. These libraries are supported under both Windows 2000 and Windows XP.

1 Using the Windows Explorer, locate and start ‘Iolibs.exe’ in the \GPIB
directory of your ChemStation CD-ROM. Follow the instructions in the
installation Wizard (see Figure 6). This will install the Standard Interface
Control Library (SICL) on your computer, update the registry and add a
new Program Group called “Agilent I/O Libraries”.

58 Installing ChemStation for LC Systems

 Installing Communication Components 3
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

Figure 6 The GPIB Driver Installation

2 Choose Next/Yes to accept the License Agreement and to view the Readme
Information. With the next step the Selection Screen for the Installation is
coming up.
3 Choose Full installation for installing the SICL libraries and VISA components
(see Figure 7). Select Next and the system will start the installation of the
SICL and VISA components of the I/O Libraries.

Installing ChemStation for LC Systems 59

3 Installing Communication Components
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

Figure 7 Choose the full SICL and VISA installation.

4 After the installation has been successful finished, select Run IO config to
configure the IO interface card.

60 Installing ChemStation for LC Systems

 Installing Communication Components 3
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

5 From the list of available interface types choose the appropriate one, i.e.
either ”82341 ISA GPIB” if you have an Agilent 82341C GPIB board installed
or “82350 PCI GPIB” in case you have the Agilent 82350 board installed (see
Figure 8).

Figure 8 Selecting the appropriate interface type in the I/O Config Screen

6 Select Configure. The configuration utility, “IOCFG.EXE”, now attempts to

detect the selected GPIB interface in the PC in order to assign the correct
resources to the card.

If the GPIB card is not detected in your PC although it is installed, proceed with the section
NOTE “Troubleshooting GPIB Interface Problems” on page 118. Or refer to the Installation
Instructions coming along with the ChemStation CD-ROM.

Installing ChemStation for LC Systems 61

3 Installing Communication Components
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

7 Set the SICL Interface Name to “hp82341”, the Bus Address to “30” and the
“System Controller” flag as shown in Figure 9. Please note that the
appearance of the dialog slightly differs depending on the type of GPIB
board installed.

You need to type in the SICL interface name hp82341 with lower case letters.
NOTE

Figure 9 Configuring the GPIB Interface for Use with the Agilent ChemStation

8 Select OK. The configured GPIB board should now be listed under
“Configured Interfaces” in the Interface Configuration Screen (Figure 10 on
page 63).

In order to be recognized by the ChemStation the SICL Interface Name has to be “hp82341”
NOTE even if you have an Agilent 82350 GPIB Interface Board installed.

62 Installing ChemStation for LC Systems

 Installing Communication Components 3
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

9 You have now successfully configured the GPIB Interface board. Restart the
the computer to finish the installation.

If you replace an Agilent 82341 board with an Agilent 82350 board or vice versa first
CAUTION
remove the configuration using the I/O Config Utility from the I/O Libraries Program
Group before exchanging the board. After the replacement you must rerun the
“IOCFG.EXE” utility to configure the correct drivers for the installed board.

Figure 10 The Interface Configuration Screen now shows the configured GPIB Board.

The interface configuration screen can be accessed from the I/O Libraries
Program Group by selecting I/O Config.

Do not attempt to start the I/O Config Utility or Visa Assistant while the ChemStation is
CAUTION
running.

Installing ChemStation for LC Systems 63

3 Installing Communication Components
Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

GPIB board Configuration with the Agilent ChemStation

Use the following values to configure the Agilent 82341 or Agilent 82350 GPIB
interface for use with the Agilent ChemStation. The available fields depend on
the type of interface installed. See Figure 9 on page 62.
SICL Interface The SICL Interface Name is the symbolic name used by the Agilent
Name ChemStation to identify and address this GPIB interface. The default Interface
Name used by the Agilent ChemStation is “hp82341”, without the quotes. The
same symbolic name has to be used for both interface types.It needs to be
typed in lower case.
VISA Interface The default VISA Interface Name is “GPIB0” (this setting is not used by the
Name Agilent ChemStation and thus does not need to be altered).
Logical Unit The Logical Unit number is used by the Standard Interface Control Library
(SICL) to uniquely identify this GPIB interface. The Logical Unit number is an
integer in the range of 0-10000. The Agilent ChemStation always uses the
default value of “7”.
Interrupt Line The Interrupt Line must be reserved for exclusive use by this GPIB interface.
(Agilent 82341 The I/O Configuration Application automatically chooses an available
only) interrupt for the card.
If you add additional accessory cards after the GPIB card was installed, you
must make sure that these cards do not use the resources reserved for the
GPIB card.
Bus Address The Bus Address determines the address of this GPIB interface on the GPIB
bus. The Agilent ChemStation uses Bus Address “30” for the System
Controller.
System Controller The System Controller determines which bus devices talk and listen. Each
device connected to the GPIB bus must have a unique GPIB bus address and
one (and only one) device acting as the System Controller. In Agilent
ChemStation installations, the System Controller is always the GPIB board
installed in the PC.

64 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

4
Installing the Analytical Instruments
Connecting an Agilent 1100 Series LC to the Agilent ChemStation 66
Connecting an HP 1090 LC to the Agilent ChemStation 72
Connecting HP 1050 Series Modules 77
Connecting Miscellaneous Instruments to the Agilent ChemStation 81
LC Instrument Firmware Requirements 83

Agilent Technologies 65
4 Installing the Analytical Instruments
Connecting an Agilent 1100 Series LC to the Agilent ChemStation

Connecting an Agilent 1100 Series LC to the Agilent ChemStation

You can connect the following modules of the Agilent 1100 Series LC to your
Agilent ChemStation using either LAN or GPIB:
• Agilent 1100 autosampler (ALS)
• Agilent 1100 well plate autosampler (WPS)
• Agilent 1100 thermostatted autosampler (ALS)
• Agilent 1100 pumping system (PMP)
• Agilent 1100 diode array detector (DAD)
• Agilent 1100 multi wavelength detector (MWD)
• Agilent 1100 variable wavelength detector (VWD)
• Agilent 1100 fluorescence detector (FLD)
• Agilent 11000 refractive index detector (RID)
• Agilent 1100 thermostatted column compartment (TCC)
• Agilent 1100 fraction collector (AS/PS)
• Agilent 1100 micro fraction collector
• Agilent 1100 vacuum degasser

Instrument cabling
The cabling setup of the Agilent 1100 system is the same for LAN and GPIB
connection. Installation and interconnection of your Agilent 1100 Series LC
modules is described in more detail in the Agilent 1100 Series Module
reference manual supplied with each module.
• Connect a controller-area network (CAN) cable between each of your
Agilent 1100 modules except the vacuum degasser. One CAN cable is
included with each Agilent 1100 module.
• Connect a remote cable (part number 5061-3378) between the Vacuum
Degasser and one other Agilent 1100 module.

66 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting an Agilent 1100 Series LC to the Agilent ChemStation

• If you are using the GPIB connection, connect the GPIB cable between one
of the Agilent 1100 modules and the GPIB interface card in your computer.

We recommend connecting the GPIB cable to an Agilent 1100 detector. If no Agilent 1100
NOTE detector is included in the system, connect it to the Agilent 1100 pumping system.

• If you are using the LAN connection, connect the Agilent 1100 module with
the JetDirect card inserted to your LAN component, using the appropriate
EtherTwist 10BaseT cable. See sections “Single instrument installation” on
page 70 and “Multiple instrument installation” on page 70 for details.

We recommend inserting the JetDirect card into a Agilent 1100 detector. If no Agilent 1100
NOTE detector is included in the system, use the Agilent 1100 pumping system.

Connecting Agilent 1100 Series Modules to the

Agilent ChemStation using GPIB
You can also use HP 1050 modules in combination with the Agilent 1100
Series modules (see “Including HP 1050 Series Modules” on page 67) when
using a GPIB connection.

Including HP 1050 Series Modules

To use HP 1050 Series modules with your Agilent 1100 Series system:
• Connect a remote cable from one of the HP 1050 Series modules to one of
the Agilent 1100 Series modules
• Connect an GPIB cable from one of the HP 1050 Series modules to the
GPIB cable on the Agilent 1100 Series module with the GPIB cable
connected.
• Connect the HP 1050 Series modules as described in “Connecting HP 1050
Series Modules” on page 77.

Installing ChemStation for LC Systems 67

4 Installing the Analytical Instruments
Connecting an Agilent 1100 Series LC to the Agilent ChemStation

Setting the GPIB Address

The Agilent 1100 modules are shipped with the default GPIB addresses as
shown in Table 9.

Table 9 Agilent 1100 Default GPIB Addresses

Agilent 1100 Module Default GPIB Address

Autosampler (ALS) 28

Isocratic pump 22

Quaternary pump 22

Binary pump 22

Capillary pump 22

Diode array detector (DAD) 26

Multi wavelength detector (MWD) 26

Fluorescence detector (FLD) 23

Refractive index detector (RID) 29

Variable wavelength detector (VWD) 24

Thermostated column compartment 27

68 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting an Agilent 1100 Series LC to the Agilent ChemStation

1. Solvent Cabinet
2. Vacuum Degasser
3. Remote Cable (5061-3378)
4. Pump
5. Autosampler
6. Controller Area Network (CAN) bus cables (5181-1516) included with each Agilent 1100
module
7. Column Compartment
8. Detector
9. 10833B GPIB cable
10. Agilent ChemStation
11. Centronics cable
12. Printer

Figure 11 Agilent 1100 System

Installing ChemStation for LC Systems 69

4 Installing the Analytical Instruments
Connecting an Agilent 1100 Series LC to the Agilent ChemStation

Connecting Agilent 1100 modules to the Agilent ChemStation using

LAN
This section gives a brief description on how to setup a dedicated mini LAN
for the analytical instruments. If you are going to connect to an existing LAN,
please contact your system administrator for further assistance.

A LAN connection requires the Agilent 1100 modules to have firmware version A.03.80 or
NOTE higher and main boards GXXXX-66520 (exchange board GXXXX-69520), were XXXX denotes
the part number of the Agilent 1100 module.

Single instrument installation

In a single instrument installation, only one instrument can be connected to
the PC and the PC can not be connected to a LAN. In this configuration, use
the black 10 ft. long crossover twisted pair cable 5183-4649, supplied with the
Agilent ChemStation software, it can only be used for the direct connection of
a single instrument to the PC.

For direct single instrument connection you can only use the cross-over cable 5183-4649
NOTE supplied with the Agilent ChemStation software. Do not use the standard Ethertwist
10BaseT cable supplied with the JetDirect card G1846A.

Multiple instrument installation

If you want to connect more than one instrument to the Agilent ChemStation
PC or if the PC needs to connect to a network, a hub (for example the G2402A
12 port hub) is required. The hub G2402A comes with one standard 8m
EtherTwist 10BaseT cable G1530-61480 that is used for the connection to the
PC. Use the standard EtherTwist 10BaseT cable G1530-61480 provided with
the JetDirect cards G1846A to connect the hub to the instruments.

Do not use the cross-over cable 5183-4649 in an installation with multiple instruments.
NOTE

70 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting an Agilent 1100 Series LC to the Agilent ChemStation

1. Personal computer
2. Network hub
3. Instruments
Use standard twisted
pair LAN cables for the
3
connections.

1 2

Figure 12 LAN connection using a hub

Setting the IP Address of the Agilent 1100 module

The IP address of the JetDirect card inserted to the Agilent 1100 module can
not be set from the Agilent 1100 system. A bootp server is required, that
provides the JetDirect card with an appropriate IP address, each time it is
started. Refer to section “Installing the CAG Bootp Server Program” on
page 52 and section “Using the Bootp Protocol” on page 109.

You need to ensure that the bootp server is running before the instrument is switched on,
NOTE as the configurational data is only loaded to the JetDirect card when the module is
switched on.

Installing ChemStation for LC Systems 71

4 Installing the Analytical Instruments
Connecting an HP 1090 LC to the Agilent ChemStation

Connecting an HP 1090 LC to the Agilent ChemStation

You can connect the following HP 1090 Liquid Chromatograph components to

your Agilent ChemStation:
• HP 1090 LC Mainframe This includes injector, pump, oven, and optionally a
filter photometric detector (FPD), and
• HP 1090 diode array detector

You can also use HP 1050 detectors in combination with the HP 1090 LC, see Figure 13 on
NOTE page 73.

Installation of your HP 1090 is described in the Installation Guide contained

in the Guide Books for the HP 1090 (part number 01090-90214).

GPIB Cabling
1 Connect an GPIB cable from the GPIB connector at the rear of the HP 1090
to the GPIB connector on the diode array detector (DAD).
2 Connect an GPIB cable from the GPIB connector on the DAD to the GPIB
connector on your computer.
The following GPIB cables are available:

Part Number Length

10833A 1m
10833B 2m
10833D 0.5 m

A 10833B cable is included with your HP 1090 Series II system and a 10833D
cable is included with your HP 1090 DAD.

72 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting an HP 1090 LC to the Agilent ChemStation

Remote Cable
Each analytical component of the system must be connected with a remote
cable (part number 01040-60202) so that time-critical events, such as
injections, can be coordinated. For example, with an HP 1090 system fitted
with a diode array detector (DAD), you must connect a remote cable between
the DAD and the rear of the HP 1090.

A remote control cable (part number 01040-60202) and an GPIB cable (part number
NOTE 10833B) are included with the diode array detector when ordered as an option to an HP
1090 system.

Some typical HP 1090 system configurations are shown in Figure 13 and

Figure 14 on page 74.

1. HP 1090 Series II/M

2. Remote cable (01040-60202) included with HP 1090M #083
3. 10833D GPIB Cable (0.5 m) included with HP 1090M #083
4. Agilent ChemStation
5. Centronics cable
6. Printer
7. 10833B GPIB Cable (2 m)

Figure 13 HP 1090 Win Liquid Chromatography System

Installing ChemStation for LC Systems 73

4 Installing the Analytical Instruments
Connecting an HP 1090 LC to the Agilent ChemStation

1. APG remote cable (5061-3378)

2. Remote cable (01040-60202) included with HP 1090M #083
3. HP 1090 Series II/M
4. 10833D GPIB Cable (0.5 m) included with HP 1090M #083
5. 10833B GPIB Cable (2 m) included with Agilent ChemStation
6. Agilent ChemStation
7. Centronics cable
8. Printer
9. 10833B GPIB Cable (2 m) included with Dual-Channel Interface
10. Two signal cables (option to Agilent 35900E)
11. 10833A GPIB Cable (1 m) included with FLD #305
12. HP 1046A Fluorescence Detector
13. APG remote cable included with FLD
14. Agilent 35900E Dual-Channel Interface
Figure 14 HP 1090 Win LC System (Includes FLD and A/D Converter)

74 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting an HP 1090 LC to the Agilent ChemStation

An example of a mixed HP 1090 and HP 1050 system configuration is shown in

Figure 15.

1. HP 1090 Series II/M

2. Remote cable (01040-60202) included with HP 1090M #083
3. 10833D GPIB Cable (0.5 m) included with HP 1090M #083
4. 10833B GPIB Cable (2 m)
5. HP 1050 Pumping System
6. Remote Cable (5061-3378) included with each HP 1050 module
7. 10833A GPIB Cable (1 m) included with each HP 1050 module
8. HP 1050 DAD
9. HP 1050 Autosampler
10. Agilent ChemStation
11. Centronics cable
12. Printer

Figure 15 HP 1090 and HP 1050 System: Multiple Instrument Version

Installing ChemStation for LC Systems 75

4 Installing the Analytical Instruments
Connecting an HP 1090 LC to the Agilent ChemStation

Setting the GPIB Address

The HP 1090 LC instrument and configurable modules are shipped with the
default GPIB addresses listed in Table 10.

Table 10 HP 1090 Default GPIB Addresses

HP 1090 Module Default GPIB Address

HP 1090 Mainframe 14

HP 79883 DAD 15

If you configure more than one module of the same type, you must change the
default settings so that each module has a unique GPIB address. For further
details, refer to the instrument handbooks.

Instructions to change the GPIB address of your HP 1090 system can be found in the
NOTE Installation Guide included with the Guide Books for the HP 1090 (part number
01090-90214).

76 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting HP 1050 Series Modules

Connecting HP 1050 Series Modules

You can connect the following modules of the HP 1050 Series LC to your
Agilent ChemStation:
• HP 1050 variable wavelength detector (VWD)
• HP 1050 multiple wavelength detector (MWD)
• HP 1050 pumping systems (PMP)
• HP 1050 autosampler (ALS)
• HP 1050 diode array detector (DAD)

You can also use HP 1050 detectors in combination with the HP 1090 LC, see Figure 13 on
NOTE page 73.

Installation of your HP 1050 Series LC modules is described in Chapter 1 of

the Installation and Maintenance handbook contained in the instruction kit
supplied with each module.
To connect the HP 1050 Series modules together to make a complete system,
see the Getting It All Together handbook contained in the instruction kit
supplied with each module.

Table 11 HP 1050 Handbooks

Instruction Kit Part Number

Pumping systems 79852-90008

Variable wavelength detector 79853-90009

Multiple wavelength detector 79854-90008

Autosampler 79855-90008

Diode array detector G1306-90000

Installing ChemStation for LC Systems 77

4 Installing the Analytical Instruments
Connecting HP 1050 Series Modules

Cabling
• Connect an GPIB cable between all your HP 1050 modules and the
Agilent ChemStation. One GPIB cable is included with the GPIB interface
accessory of each HP 1050 module.
The following GPIB cables are available:

Part Number Length

10833A 1m
10833B 2m
10833D 0.5 m

• It is recommended that GPIB devices are connected in a daisy-chain

configuration in which each device is connected to the previous one using
an GPIB cable.
• Avoid using configurations in which all devices are connected to a central
point, particularly when cables longer than three meters are used.

Remote Cable
Each analytical component of the system must be connected with an APG
remote cable (part number 5061-3378) so that time-critical events, such as
injections, can be coordinated. Connect all modules together in a daisy chain.
For further GPIB connection details, see your HP 1050 Series Installing and
Maintaining handbook contained in the Instruction Kit included with each
module.

78 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting HP 1050 Series Modules

Setting the GPIB Addresses

The HP 1050 modules are shipped with the default GPIB addresses as shown
in Table 12.

Table 12 HP 1050 Default GPIB Addresses

HP 1050 Module GPIB Address

Variable wavelength detector (VWD) 10

Isocratic and quaternary pumps (PMP) 16

Multiple wavelength detector (MWD) 17

Autosampler (ALS) 18

Diode array detector (DAD) 17

If you configure more than one module of the same type, you must change the
default settings so that each module has a unique GPIB address. See the
Installing and Maintaining handbooks, included with each analytical module,
for information on setting the GPIB address.
Two examples of HP 1050 system configurations are shown in Figure 16 and
Figure 17.

Figure 16 Agilent ChemStation with HP 1050 Diode Array Detector

Installing ChemStation for LC Systems 79

4 Installing the Analytical Instruments
Connecting HP 1050 Series Modules

1. HP 1050 Pumping System

2. 10833B GPIB Cable (2 m)
3. Agilent ChemStation
4. Printer
5. Centronics cable
6. 10833A GPIB Cable (1 m) included with VWD
7. 10833D GPIB Cable (0.5 m) included with Autosampler
8. 10833B GPIB Cable (2 m) included with Dual-Channel Interface
9. Two signal cables (option to Agilent 35900E)
10. Agilent 35900E Dual-Channel Interface
11. Remote Cable (5061-3378) included with each HP 1050 module
12. HP 1050 Autosampler
13. HP 1050 VWD

Figure 17 HP 1050 System

80 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
Connecting Miscellaneous Instruments to the Agilent ChemStation

Connecting Miscellaneous Instruments to the

Agilent ChemStation

In addition to the HP 1050 Series, HP 1090 and Agilent 1100 Series, you can
connect the following stand-alone analytical instruments to your
Agilent ChemStation:
• HP 1040 diode array detector
• HP 1046 fluorescence detector
• Agilent 35900 dual channel interface
• HP 1049 electrochemical detector
To install these instruments, see the operator’s handbook shipped with each
instrument.
The HP 1047A refractive index detector cannot be connected via GPIB. You
need a 35900 dual channel interface to acquire data from the HP 1047A.

Setting the GPIB Addresses

These instruments are shipped with the following default GPIB addresses
shown in Table 13.

Table 13 Miscellaneous Instrument Default GPIB Addresses

LC Module Default GPIB Address

HP 1040A DAD 15

HP 1046A FLD 12

HP 1049A ECD 11

Agilent 35900E dual 13

channel interface

Installing ChemStation for LC Systems 81

4 Installing the Analytical Instruments
Connecting Miscellaneous Instruments to the Agilent ChemStation

If you configure more than one module of the same type, you must change the
default settings so that each module has a unique GPIB address. For further
details, please refer to the instrument handbooks.

Remote Control Cabling

All modules of an instrument that accept timetable entries or which go into
the run mode during an analysis must be included in the remote control loop.
In general, each module must be connected to the other devices with a remote
control cable. The HP 1050 modules, the HP 1046A FLD, the HP 1049A ECD,
and the HP 1090 Series II include a dual-APG remote connector with
pass-through capability. This means that you may use one remote connector as
an input and the other as an output. All remote signals which enter these
connectors pass through to the other connector, as well as being seen by the
module.
The 5900E dual channel interface use APG remote, but the two remote
connectors are not pass-through connections. When you operate the 35900E
with both channels, only the A-channel remote will be connected. The
B-channel acts in synchronization with the A-channel and will react to the
remote signals from the A-channel remote connector. The only time you
connect a remote cable to the B-channel remote connector is if you wish to
operate the B-channel independently. In this mode, one instrument may start
and stop the A-channel while another instrument has control of the B-channel.
The built-in DAD of the HP 1090 Series I LC and the stand-alone HP 1040A
DAD do not include standard APG remote connections. It is necessary to
connect the remote connection of the HP 1090 mainframe to the remote
connector of the built-in or stand-alone DAD, using a single flat-connector
remote cable (part number 01040-60202).
When connecting additional devices (that typically support APG-remote) to an
HP 1090 system, a signal distribution module must be used.

82 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
LC Instrument Firmware Requirements

LC Instrument Firmware Requirements

The LC ChemStation software will require minimum firmware revisions in

order to work with the following devices shown in Table 14.

Table 14 LC Instrument Firmware Requirements

LC Instrument Product Number Firmware Revision Part Number

HP 1050 isocratic pump 79851A SFW board rev. 3.1 (3027) or higher 01018-66506
HP 1050 quaternary pump 79852A SFW board rev. 3.1 (3027) or higher 01018-66506
HP 1050 VWD 79853A, C GPIB ROM rev. 0.20 79853-13004
HP 1050 VWD 79853A 3.02 79853-13002
HP 1050 VWD 79853C 4.24 79853-13005
HP 1050 VWD 79853C 4.31 79853-13000
(S/N prefix > 3522)
HP 1050 MWD 79854A 3.1 (3027) or higher 01048-66504
HP 1050 DAD G1306A 1.0 or higher G1306-69524
HP 1050 autosampler 79855A FIM rev. 4.1 01078-66506
HP 1090L MEM D-3014 01090-66573
HP 1090L LIB E-3049 01090-66579
HP 1090M MCO all revisions 01090-66542
HP 1090M MEM D-3014 01090-66573
HP 1090 II/L LIB E-3049 01090-66579
Agilent 1100 autosampler G1313A A.03.80 or newer n.a.*
Agilent 1100 micro sampler G1389A A.04.10 or newer n.a.†
Agilent 1100 preparative autosampler G2260A A.05.10 or newer n.a.‡
Agilent 1100 well plate autosampler G1367A A.04.13 or newer n.a.**
Agilent 1100 micro well plate autosampler G1377A A.04.13 or newer n.a.††
Agilent 1100 isocratic pump G1310A A.03.80 or newer n.a.*

Installing ChemStation for LC Systems 83

4 Installing the Analytical Instruments
LC Instrument Firmware Requirements

Table 14 LC Instrument Firmware Requirements (continued)

LC Instrument Product Number Firmware Revision Part Number

Agilent 1100 quaternary pump G1311A A.03.80 or newer n.a.*
Agilent 1100 binary pump G1312A A.03.80 or newer n.a.*
Agilent 1100 capillary pump G1376A A.04.11 or newer n.a.*
Agilent 1100 preparative pump G1361A A.04.17 or newer n.a.*
Agilent 1100 DAD G1315A A.03.80 or newer n.a.*
Agilent 1100 DAD G1315B A.04.11 or newer n.a.*
Agilent 1100 MWD G1365A A.03.80 or newer n.a.*
Agilent 1100 MWD G1365B A.04.11 or newer n.a.*
Agilent 1100 FLD G1321A All Revisions n.a.*
Agilent 1100 VWD G1314A A.03.80 or newer n.a.*
Agilent 1100 RID G1362A A.03.80 or newer n.a.*
Agilent 1100 Fraction Collector G1364A A.05.05 or newer n.a.*
Agilent 1100 Fraction Collector PS G1364B A.05.06 or newer n.a.*
Agilent 1100 Fraction Collector AS G1364C A.05.06 or newer n.a.*
Agilent 1100 Micro Fraction Collector G1364D A.05.06 or newer n.a.*
Agilent 1100 degasser G1322A All Revisions n.a.*
Agilent 1100 local user interface G1323A A.02.01 n.a.*
HP 1040A or HP 1040M DAD COM board firmware rev. E-3014 79880-13007
HP 1040A or HP 1040M DAD DAP board firmware rev. B 79880-69504
dual grating FLD HP 1046A FDC board rev. B-2630 01046-66503
dual grating FLD HP 1046A FDI board with firmware rev. B or C 01046-69509
Electrochemical Detector HP 1049A Firmware rev. E-3314 01049-13002
HP 1090 DAD option #080 COM board firmware rev.E-3014 79880-13007

* The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.
† The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.
‡ The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

84 Installing ChemStation for LC Systems

 Installing the Analytical Instruments 4
LC Instrument Firmware Requirements

** The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.
†† The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

A LAN connection requires the Agilent 1100 modules to have firmware version A.03.80 or
NOTE higher. The main board (DAM) of the module with the JetDirect card inserted must be
GXXXX-66520 (exchange board GXXXX-69520), were XXXX denotes the part number of the
Agilent 1100 module.

If there is a CTL board in the HP 1090, replace it with the assembly kit MCO/MEM board
NOTE 01090-69708

Pre-1985 HP 1090 LCs require the following new boards: COM board (79880-69516) and
NOTE DAP board 79880-69504.

When connecting an HP 1046A and/or a 35900 A/D dual channel interface to an HP 1090M
NOTE or HP 1090L (that is, prior to Series II), use the cabling kit 01090-68700 (includes signal
distribution module 01090-60302, two remote cables 01040-60202).

Installing ChemStation for LC Systems 85

4 Installing the Analytical Instruments
LC Instrument Firmware Requirements

86 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

5
Checking Out the Agilent ChemStation
Agilent ChemStation Installation Verification 88
Qualifying Operation — Agilent ChemStation Verification Test 94

Agilent Technologies 87
5 Checking Out the Agilent ChemStation
Agilent ChemStation Installation Verification

Agilent ChemStation Installation Verification

Overview
After installing the Agilent ChemStation system software on your computer
and configuring the analytical system, your internal validation procedure may
require you to assess the correctness and completeness of the installation and
to verify that the analytical system is fully operational.

Installation Qualification
The Agilent ChemStation Installation Verification utility uses factory
delivered installation reference files to verify the existence, correctness and
integrity of the required Agilent ChemStation system files (executable
program files, binary register files, macro files, initialization files, help files,
customized report templates). This process is called Installation Qualification
(IQ).
File integrity is verified by comparing the 16-bit cross-redundancy-check
(CRC) checksum of the installed file with the checksum of the original file
recorded on Agilent Technologies’s installation master. The file details of the
installation master are delivered on so-called reference files. Modified or
corrupted files have different checksums and are thus detected by the IQ
utility.
The integrity of the reference files themselves is also tracked with the help of
checksums. In case the installation verification utility is supplied with a
reference file that was modified after its generation, this will be flagged in the
report (section invalid reference files).
In addition, the IQ utility checks the version code of the Agilent ChemStation
executable system files (*.EXE, *.DLL).

88 Installing ChemStation for LC Systems

 Checking Out the Agilent ChemStation 5
Agilent ChemStation Installation Verification

Adding the Agilent ChemStation Installation Verification Utility

The Agilent ChemStation Installation Verification is automatically installed
and the appropriate IQ reference files are copied to your PC’s hard-disk and
the appropriate Agilent ChemStation IQ icons are added to your Agilent
ChemStation group.

Agilent ChemStation Installation Verification Utility Execution

Double-click on the “Installation Qualification” icon in the
Agilent ChemStation group.

Make sure the Agilent ChemStation is closed before running the Installation Verification
NOTE Utility.

The checksum calculation for all Agilent ChemStation system files may take
several minutes. A dialog box indicates the name of the system file that is
currently being analyzed.

Installing ChemStation for LC Systems 89

5 Checking Out the Agilent ChemStation
Agilent ChemStation Installation Verification

Agilent ChemStation IQ Utility Results

The Agilent ChemStation Installation Verification utility displays the
verification results on the screen and can optionally generate a report of its
findings.
On a complete and consistent installation, the installation verification
completes without any error messages and no files are reported missing or
modified.
If the verification report lists files as missing or modified, the installation
verification utility displays the error message Installation Verification
completed with error(s) in the message line.
The Agilent ChemStation Installation Verification reports the following file
categories:

Table 15 File Categories Reported by Agilent ChemStation Installation Verification

File Category Explanation Required Action

Identical files Required files exist and passed No action required.

the version and integrity check.

Missing files files are missing that are Reinstall the Agilent ChemStation.
required to run the
Agilent ChemStation.

Modified files Files have been corrupted or Reinstall unless you have intentionally
modified. customized or updated
Agilent ChemStation files; Generate a
new installation verification reference
file for customized or updated Agilent
ChemStation installations.

Invalid reference file The original reference file is Reinstall the original reference file
corrupt or has been modified
after creation.

90 Installing ChemStation for LC Systems

 Checking Out the Agilent ChemStation 5
Agilent ChemStation Installation Verification

Figure 18 Example for System Modifications Detected by Installation Verification

Installing ChemStation for LC Systems 91

5 Checking Out the Agilent ChemStation
Agilent ChemStation Installation Verification

The printed report contains the license registration numbers used on the
system that was checked. It also lists the reference files (name and date code)
that were used for the installation verification.
Example report:
ChemStation Installation Verification

Installed licenses
XM00000000

Reference files
sys\lc3d.ref (14. Feb 2001, 12:43)

Identical files
(...)
hpced02.exe 1.1.5.1 238B HP APG Configuration Editor
hpfabs00.dll 1.0.3.3 9BC0 support functions
(...)

92 Installing ChemStation for LC Systems

 Checking Out the Agilent ChemStation 5
Agilent ChemStation Installation Verification

Generating an Installation Verification Reference File on a

Customized Installation
The Agilent ChemStation has an open architecture that allows knowledgeable
users to add their own customization code. In regulated environments, it is
general practice to document such adaptations, additions or modifications.
For your own tracking purposes, you can use the Agilent ChemStation
installation verification utility to generate a reference file listing for the
customized system. You can use this reference file to qualify the completeness
and integrity of the customized Agilent ChemStation and use it as a tracking
document for future modifications. The program scans for files with the
extensions {*.dlc, *.dll, *.drv, *.enu, *.exe, *.hlp, *.ini, *.mac, *.mcx}.
For example, in order to create the file contents list along with the
corresponding file checksums for your existing installation, execute the
Agilent installation verification utility with the following command line
options:
C:\HPCHEM\SYS\HPVERI00.EXE -f custom.ref
Add the resulting reference file to your project documentation. To
automatically run an installation qualification test using the new reference
file, set up an icon with the following command line specification in its
properties:
hpveri00.exe -r custom.ref
For a complete description of the command line syntax of the installation
verification utility, refer to its help file (“C:\HPCHEM\SYS\HPVERI.HLP”).

See the installation verification help system for a complete description of the installation
NOTE verification utility’s command line options. The installation verification does not check
method files, sequence files or data files. These files are stored in Agilent Technologies
proprietary, unalterable binary formats and are checked for integrity when loaded into the
Agilent ChemStation.

Installing ChemStation for LC Systems 93

5 Checking Out the Agilent ChemStation
Qualifying Operation — Agilent ChemStation Verification Test

Qualifying Operation — Agilent ChemStation Verification Test

Overview
The Agilent ChemStation provides a verification test function that checks out
the system’s software operation. Using either Agilent-supplied or
user-generated data files and methods, the system will match calculated
integration and quantification results with ones that have been previously
generated and archived in an unalterable, checksum-protected, binary register
file.
When using an HP 1050 DAD (product number HP G1306A), an Agilent CE
instrument with a built-in DAD (product number Agilent G1600A) or an
Agilent 1100 Series detector, the verification test can be extended to include a
data acquisition check. The verification test generates a report that includes a
list of the tested configuration, the verification test details like the data file
and method used, and the test results that indicate whether the system passed
a specific test step or not.

Performing the Verification Test

• After installing the Agilent ChemStation and successfully passing the
Installation Verification, start the Agilent ChemStation
• Select Run Test from the Verification View and run the verification test
procedure called default.val.
• Please refer to the analytical tasks section of online help system if you
require further information.

Acceptance Criteria
The test was passed successfully, if the verification test report indicates that
all individual test steps were passed. This proves that internal components
such as the Command Processor, Method Handler, the internal Memory
Manager for data objects, registers and tables, the Data Analysis modules for
peak identification, peak quantification, report formatting and the
Agilent ChemStation print spooler are fully operational.

94 Installing ChemStation for LC Systems

 Checking Out the Agilent ChemStation 5
Qualifying Operation — Agilent ChemStation Verification Test

If the test fails, the verification test report indicates which part of the test did
not meet the acceptance criteria. Verify you used the correct verification
procedure. If the verification test continues to fail, we recommend that you
reinstall the Agilent ChemStation. Do not delete your current system
installation, as the installation procedure will copy the original
Agilent ChemStation files on top of the existing ones.

Installing ChemStation for LC Systems 95

5 Checking Out the Agilent ChemStation
Qualifying Operation — Agilent ChemStation Verification Test

96 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

6
Configuring the Analytical System
Using the Agilent ChemStation Configuration Editor 98
Example for LC Configurations 102
Using the Bootp Protocol 109

Agilent Technologies 97
6 Configuring the Analytical System
Using the Agilent ChemStation Configuration Editor

Using the Agilent ChemStation Configuration Editor

What is the Agilent ChemStation Configuration Editor?

The Agilent ChemStation Configuration Editor is a program that allows the
easy configuration of your Agilent ChemStation software. This includes
• Detecting the GPIB interface in your PC
• Selects communication using LAN or GPIB
• Configuring the analytical hardware connected to the PC.
• Configuring the path used for method, data and sequence storage.
• Configuring color display of the Agilent ChemStation.
After installing the Agilent ChemStation software, you are prompted to
configure the complete analytical system.
The Agilent ChemStation Configuration Editor needs to be run every time a
GPIB device is connected to or disconnected from the GPIB bus of the PC.
Since the Configuration Editor modifies the internal communication module
database, the Agilent ChemStation must be closed beforehand.
For a LAN connection, the configuration editor only needs to be run, if the IP
address of the instrument is changed.

98 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Using the Agilent ChemStation Configuration Editor

Starting the Configuration Editor

Start the Agilent ChemStation Configuration Editor by double-clicking on the
Configuration Editor icon in the Agilent ChemStation start-up group or by
running “C:\HPCHEM\HPCED02.EXE” from the Start/Run menu.

If GPIB connection is used, a GPIB interface must be installed in the PC, except for the
NOTE Agilent ChemStation Data Evaluation Only products, Agilent G2090AA and Agilent
G2190AA. The I/O Config utility “IOCFG.EXE” must have been run to configure the
interface.

If LAN connection is used, Microsoft TCP/IP protocol must be installed and configured on
NOTE the Agilent ChemStation PC. In addition a bootp server must be running and configured for
the instruments to be used.

To avoid communication errors on the GPIB bus, we strongly discourage you from
CAUTION
powering modules on or off while the Agilent ChemStation is running.

What You Do
• Select/configure the GPIB interface (for GPIB communication) or setup the
bootp server (for LAN communication).
• Configure the instrument type (1090 system, modular system, data analysis
only, all with or without spectral capabilities).
• Configure the instrument devices, like special detectors, access points, etc.
• Configure the paths used for methods, sequences and data files (optional).
• Configure the colors used for chromatograms, titles and baselines
(optional).
• Save your configuration.

Installing ChemStation for LC Systems 99

6 Configuring the Analytical System
Using the Agilent ChemStation Configuration Editor

Select and Configure the GPIB Interface

To establish communication through the GPIB bus, the interface configuration
must be set in the Configuration Editor. These settings are not necessary if
only a LAN connection is used.
We recommend that you check your GPIB card has the correct default settings.
See “Installing an GPIB Interface Board in the PC” on page 56 for more
information.
1 Choose GPIB Card from the Configure menu. The Configuration Editor
attempts to detect the installed card.
2 Either for the Agilent 82341 or the Agilent 82350 GPIB interface, select
“hp82341” .
3 Choose OK to save changes and return to the Configuration Editor display.

Configuring Paths
The Configuration Editor lets you specify alternate path settings for your
sequences, methods, and data files. This allows storing data files on a separate
drive.

Before you modify the path settings using the Configuration Editor, you need to create the
NOTE appropriate directories.

Do not delete the default method and sequence (“DEF_XX.S” and “DEF_XX.M”, where
CAUTION
XX stands for the technique (LC, GC or CE). They are used as a template for new
sequences and methods.

100 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Using the Agilent ChemStation Configuration Editor

This section describes how to configure different paths for your methods,
sequences, and data files. The Agilent ChemStation default path settings are
also listed.
1 Choose Paths from the Configure Menu.
2 Set your data file path. Default = C:\HPCHEM\1\DATA\
3 Set your method path. Default = C:\HPCHEM\1\METHODS\
4 Set your sequence path. Default = C:\HPCHEM\1\SEQUENCE\
5 Choose OK to update the path information and return to the Configuration
Editor display.

All path names have to be terminated with a backslash \.

NOTE

Installing ChemStation for LC Systems 101

6 Configuring the Analytical System
Example for LC Configurations

Example for LC Configurations

Configuring an Agilent ChemStation Instrument

In most cases, the Agilent ChemStation instrument configuration information
has to be adapted to match the connected analytical hardware after the
installation of the Agilent ChemStation application software. Most Agilent
Technologies analytical hardware is modular and can be combined in various
ways. The following instrument types are available for an LC configuration:

Table 16 LC Instrument Types in the Agilent Configuration Editor

Instrument Type Supported LC Hardware Data Evaluation Capabilities LAN

1090 3D LC system HP 1090 DAD spectra evaluation included No

1090 LC system HP 1090 no spectral evaluation included No

LC 3D data analysis only no instrument control spectra evaluation included No

LC data analysis only no instrument control no spectra evaluation included No

Modular 3D LC system HP 1050 spectra evaluation included No

Modular LC system HP 1050 no spectra evaluation included No

Modular 3D LC system Agilent 1100 spectra evaluation included Yes

Modular LC system Agilent 1100 no spectra evaluation included Yes

102 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Example for LC Configurations

Configuring an Agilent 1100 Series LC System

The following example describes how to configure an Agilent 1100 Series
system with modules connected together by the Agilent 1100 Series controller
area network (CAN) that will automatically configure each module.
This section describes how to set up the configuration of your Agilent 1100
Series system.
1 Start the Agilent ChemStation Configuration Editor.
2 Select the Configure/Instruments menu.
3 Select the appropriate instrument type (“Modular LC System” or “Modular
3D LC System”)).
4 Assign a name to your analytical instrument (e.g. Joe’s LC).
5 Select NO for initially start instrument session. This allows you to start your
instrument using the appropriate Agilent ChemStation icon.
6 Specify your preferred screen window size (Normal, Icon, Full Screen).
7 Press OK.
8 In the Device Configuration dialog, select “1100 System Access” from the
list (see Figure 19 on page 104). In the case of a GPIB connection, select the
GPIB radio button and select the GPIB address of the module the GPIB
cable is connected to, this is the system access GPIB address. In case of
LAN access select the LAN radio button.
For the list of default GPIB addresses for Agilent Technologies analytical
hardware, press the Help button in the Device Configuration dialog box.
9 Select Add to add the selected system access to the list of configured
modules. If LAN access was selected a type in the IP address (or host name
if name resolution is implemented on your network) of the Agilent 1100
module with the LAN connection.
10 Select OK to exit and return to the Configuration Editor display.
11 This step is applicable for online instruments using GPIB connection only.
For data analysis only configurations or LAN connections, proceed with the
next step. Select Configure/GPIB card. The Configuration Editor attempts
to detect an GPIB interface in your system. If you obtain a warning message

Installing ChemStation for LC Systems 103

6 Configuring the Analytical System
Example for LC Configurations

“No GPIB cards found”, install and/or configure an GPIB interface as

described in “GPIB Communication” on page 53.
12 Save your configuration changes by selecting File/Save.
13 This step is applicable for online instruments using LAN connection only.
For data analysis only configurations, proceed with the next step. If your IT
department did not set up a bootp server for you, you need to do this
yourself.
14 When you start the Agilent ChemStation for the first time, the “Configure
1100 System Access” dialog box displays the Agilent 1100 modules detected
during instrument initialization (see Figure 20 on page 105). Agilent 1100
modules that have been recognized are listed as “online” (indicated by a
green icon) along with their serial numbers. Agilent 1100 modules that have
not been detected are marked “offline”. You can selectively take certain
modules out of the software configuration, e.g. if your Agilent 1100 system
includes two Agilent 1100 detectors and your next analysis only requires
one of them. The unneeded Agilent 1100 module may then remain turned
off but still be connected. You may also re-order modules, for example if you
wish to use one pump as the primary analytical pump and another as a
make-up pump. After modifying the configuration, you must restart the
Agilent ChemStation. The “Configure 1100 System Access” dialog is
available from the Instrument menu in the “Method and Run-Control” view,
when Full Menus are displayed.

Figure 19 Agilent ChemStation Configuration Editor: Device Configuration Dialog

104 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Example for LC Configurations

Figure 20 The Configure Agilent 1100 System Access dialog allows to selectively include
detected Agilent 1100 modules into the configuration.

Configuring an HP 1050 LC System

You can configure the following modules for use with the Agilent ChemStation:
• HP 1050 Series isocratic pump
• HP 1050 Series quaternary pump
• HP 1050 Series autosampler
• HP 1050 Series diode array detector
• HP 1050 Series multiple wavelength detector
• HP 1050 Series variable wavelength detector
• HP 1046 fluorescence detector
• HP 1049 electrochemical detector
• 35900 dual channel interface

Installing ChemStation for LC Systems 105

6 Configuring the Analytical System
Example for LC Configurations

This section describes how to configure an HP 1050 Series system with the
following devices:
• HP 1050 quaternary pump
• HP 1050 autosampler
• HP 1050 variable wavelength detector

Configuring Your Instrument Devices

This section describes how to set up each device that you have connected to
build your instrument.

106 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Example for LC Configurations

1 Start the Agilent ChemStation Configuration Editor.

2 Select the Configure/Instruments menu.
3 Select the appropriate instrument type (e.g. 1050 3D LC System).
4 Assign a name to your analytical instrument (e.g. Joe’s LC).
5 Select NO for initially start instrument session. This allows you to start your
instrument using the appropriate Agilent ChemStation icon.
6 Specify your preferred screen window size (Normal, Icon, Full Screen).
7 Press OK.

This example assumes an HP 1050 system consisting of a quaternary pump, autosampler

NOTE and VWD. When you do this yourself, select the modules appropriate to your HP 1050
configuration.

8 Choose the HP 1050 Series quaternary pump from the Modules list box.
9 Set the GPIB address in the GPIB address list box. For the HP 1050
quaternary pump the default GPIB address is 16.

Choose Help to view a list of the default GPIB addresses, or see Table 12 on page 79.
NOTE

10 Choose Add from the main menu to add the HP 1050 quaternary pump in
the Selected Modules list box.
11 Choose the HP 1050 autosampler from the Modules list box, set the GPIB
address (default is 18) and choose Add to add the HP 1050 autosampler in
the Selected Modules list box.
12 Choose the HP 1050 variable wavelength detector from the Modules list box,
set the GPIB address (default is 10) and choose Add to add the HP 1050
VWD in the Selected Modules list box.
13 Choose OK to exit and return to the Configuration Editor display.
14 Save your configuration changes by selecting File/Save.

Saving Configuration Changes

1 Choose File from the Configuration Editor menu.
2 Choose Save from the File menu. Your configuration has now been saved.

Installing ChemStation for LC Systems 107

6 Configuring the Analytical System
Example for LC Configurations

Configuring an HP 1090 LC System

The following example describes how to configure an HP 1090 system with the
following devices:
• HP 1090 PV5 solvent delivery system
• HP 1090 autosampler
• HP 1090 diode array detector

Configuring Your Instrument Devices

This section describes how to set up each device that you have connected to
build your instrument.
1 Start the Agilent ChemStation Configuration Editor.
2 Select the Configure/Instruments menu.
3 Select the appropriate instrument type (e.g. 1090 3D LC System).
4 Assign a name to your analytical instrument (e.g. Joe’s LC).
5 Select NO for initially start instrument session. This allows you to start your
instrument using the appropriate Agilent ChemStation icon.
6 Specify your preferred screen window size (Normal, Icon, Full Screen).
7 Press OK.
8 Set the GPIB address of your HP 1090 mainframe (default is 14).
9 Select the HP 1090 diode-array detector from the Modules list box.
10 Set the GPIB address in the GPIB address list box (default is 15).
11 Choose Add from the main menu to add the HP 1090 diode array detector
in the Selected Modules list box.

If your HP 1090 uses a filter photometric detector (FPD), you must configure an Agilent
NOTE 35900C or 35900E dual channel interface to convert the analog detector signals to digital
signals for the Agilent ChemStation.

12 Select OK to exit and return to the Configuration Editor display.

13 Save your configuration changes by selecting File/Save.

108 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Using the Bootp Protocol

Using the Bootp Protocol

What is the Bootp Protocol

Bootp (Bootstrap Protocol) is used for the central administration and
distribution of IP addresses and settings required by devices on a network. For
these processes to take place, a Bootp server must be running on the network.
In case of the instrument on the LAN, the HP JetDirect card used to connect
the Agilent 1100 modules to the LAN does not store any settings, so each time
the instrument is powered on the card sends a request to the network for an IP
address and setting information. The implementation of the bootp protocol on
the JetDirect card conforms to RFC 951 and RFC 1048.
In order to provide this information to the card, the bootp server must be
operational before the card sends the request and the bootp server must be
configured to know the hardware address (also called MAC address) of the
JetDirect card. Multiple JetDirect cards can be configured with a single bootp
server.
If your network is already using a bootp server, please refer to section
“Configuring the JetDirect Card Using Bootp” on page 112. If you do not have a
bootp server, we suggest to use the CAG bootp server program supplied on the
Agilent ChemStation Software family CD-ROM.

Installing ChemStation for LC Systems 109

6 Configuring the Analytical System
Using the Bootp Protocol

Configuring the CAG Bootp Server Program

The CAG bootp server program allows easy configuring of the JetDirect card.
Selecting the item Configure -> Add entry allows to easily add a MAC address
and IP address pair to the configuration. Using the
Configure > Bootp Manager... item, a list of the currently configured MAC
address is displayed and can easily be modified.

Getting the MAC Address

The MAC or hardware address of the JetDirect is a world wide unique
identifier. No other network device will have the same hardware address. If
the MAC address is not printed on the card, perform the following steps
1 Connect the instrument on the LAN to the PC, preferably this should be the
only network devices on the network, using the set up described in the
section “Single instrument installation” on page 70.
2 Start the bootp server.
3 Open the bootp server window by clicking on it in the taskbar.
4 The window will display the current bootp requests from the total network.
If many bootp requests are visible, try to isolate the network segment the
PC and the instrument on the LAN are on.

110 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Using the Bootp Protocol

5 Now switch on the instrument on the LAN and view the bootp window.
After 2–10 s the bootp server will display the request from the JetDirect
card with the hardware address (this information is also stored in the file
trace.txt in the bootp server directory, if Log to Disk is enabled from the
File menu).
6 Select Configure > Default Settings... and specify the subnet mask and
default gateway, in the given example for an isolated connection the subnet
mask is 255.255.255.0 (the default gateway setting is not required). Confirm
the entry with OK.
7 Select Configure > Add Entry... to add an entry. The drop down box
“MAC address” lists all MAC addresses that recently send a bootp request.
Select the one for the JetDirect card and make the required settings. Select
OK to add the entries.

Installing ChemStation for LC Systems 111

6 Configuring the Analytical System
Using the Bootp Protocol

8 Power cycle the analytical instrument, to force it to send again a bootp

request. This time, the MAC address will be found by the bootp server and
the CAG Bootp Server window will display the following.

Configuring the JetDirect Card Using Bootp

In order for an internal JetDirect card to use bootp, the default setting:
bootp=YES must be set. This is the default setting from the manufacturer and
can be viewed on a Agilent 1100 system using the Agilent 1100 control module.
In the System view of the control module, select Configure > MIO for the
module where the JetDirect card is inserted and scroll down to corresponding
item.

When the MIO dialog is opened on the Agilent 1100 control module, the ChemStation can
NOTE not communicate with the Agilent 1100 system.

To change the setting, the card must be reset; this is done each time the
Agilent 1100 module with the JetDirect card inserted is power cycled.
A bootp server uses a file called bootptab were all the necessary information is
stored. The bootp server program on the Agilent ChemStation CD-ROM allows
easy editing of the bootptab file. If a bootp server is already installed, the
following information must be supplied to the system administrator to be able
to correctly set up the bootptab file.

112 Installing ChemStation for LC Systems

 Configuring the Analytical System 6
Using the Bootp Protocol

# LC1100 JetDirect Card - Instrument 1

LC1100-1:\
ht=1:\
ha=0060B0047394:\
ip=10.10.10.2:\
T145=64:T146=01:T147=01
The entries are described below
• # LC1100 JetDirect Card - Instrument 1
Every entry on a line after a # is a comment and ignored by the bootp
server.
• LC1100-1:\
Host name of the instrument. The host name communication can only be
used if a DNS server (Domain name server) is correctly setup for the name
or a corresponding entry in the HOSTS file exists (windows\system32\
drivers\etc\hosts).
• ha=0060B0047394:\
This is the hardware or MAC address of the JetDirect card. It is usually
printed on a label attached to the card. If not, see section “Getting the MAC
Address” on page 110.
• ip=10.10.10.2:\
This is the IP address that will be send to the card. This address needs to be
set in the Agilent ChemStation Configuration Editor.
• T145=64:T146=01:T147=01
These parameters set the buffer handling of the JetDirect card. These are
required for the instrument on the LAN to work properly and should not be
changed.
Additionally entries for the subnet mask and default gateway might be
required, depending on your network setup. These values need to be set by
your network administrator.

Installing ChemStation for LC Systems 113

6 Configuring the Analytical System
Using the Bootp Protocol

114 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

7
Troubleshooting
Troubleshooting LAN Communication 116
Troubleshooting GPIB Interface Problems 118
Software Start-up Problems 121
Printing Problems 126
Computer Problems 130
LC Instrument Start-up Problems 132

Agilent Technologies 115

7 Troubleshooting
Troubleshooting LAN Communication

Troubleshooting LAN Communication

Power fail reported on the Agilent ChemStation

If your ChemStation can not connect to the analytical instrument configured
using a LAN communication, please perform the following troubleshooting
steps.

Verify that the IP Address is correct

Check the IP address and subnet mask of the Agilent 1100 system by using the
control module, if available. In the System view of the control module, select
Configure->MIO for the module where the JetDirect card is inserted and scroll
down to the IP address of the JetDirect card.

When the MIO dialog is opened on the Agilent 1100 control module, the ChemStation can
NOTE not communicate with the Agilent 1100 system.

Verify that Basic Communication is possible

Use the ping command, to verify that the IP address is operational.
1 Open a command prompt window on your PC.
2 Type “ping 10.10.10.2” and press Enter; where 10.10.10.2 needs to be
replaced by the appropriate IP address or by the selected host name.
3 The command ping will send a request for reply to the IP address, bypassing
part of the Windows TCP/IP settings. A successful ping would look like
Reply from 10.10.10.2: bytes=32 time<10ms TTL=128
If “request timed out” would be displayed, the IP address can not be
reached by the ping command.
4 If the ping request was answered successfully by the instrument, you need
to verify that your Windows TCP/IP settings are correct for the selected
network, especially the subnet mask and gateway settings should be
checked.

116 Installing ChemStation for LC Systems

 Troubleshooting 7
Troubleshooting LAN Communication

Identify by host name

If you Identify the Agilent 1100 system by host name, ensure that the used
host name and IP address are correctly setup in the DNS server or the
corresponding entry in the HOSTS file exists, see “Configuring the JetDirect
Card Using Bootp” on page 112. Try using the IP address in the Configuration
Editor.

Verify that the JetDirect Card is correctly configured

To ensure that all parameters of the JetDirect card are correctly set, use the
CAG Bootp server program from the ChemStation CD-ROM and disable any
other means that configure the JetDirect card of the instrument. Install the
bootp server as described in section “Installing the CAG Bootp Server
Program” on page 52 and configure it for the MAC address of the JetDirect
instrument. Leave the bootp server open, power cycle the instrument and
verify that the bootp server configures the JetDirect card correctly. Thus you
ensure that the parameters for the buffer handling in the JetDirect card are set
for analytical instruments.

DHCP Server
Check that no DHCP sever runs in the same subnet as the analytical
instruments, as these servers also respond to bootp request and do not set the
buffer handling of the JetDirect card correctly. They also may send a different
IP address to the instrument each time it is started.

Frequent Buffer Overrun in Instrument Log Book

Please contact your system administrator, to check if the network is not
capable of the network traffic induced due to the data acquisition of the
instruments. This can also be caused by an incorrectly configured JetDirect
card, see above.

Installing ChemStation for LC Systems 117

7 Troubleshooting
Troubleshooting GPIB Interface Problems

Troubleshooting GPIB Interface Problems

The most common problem encountered when using I/O Config is that an
interface is not found. For an Agilent 82341 or Agilent 82350 GPIB interface,
this is due to one of the following reasons:

Figure 21 No GPIB Interface Was Detected on the System

• The interface was not installed in the PC before running I/O Config. Install
the GPIB interface card in your system and then run I/O Config again.
• The interface is not firmly seated. Ensure that the board is in place and the
boards edge connectors ar fully seated. Please refer to the section
“Installing an GPIB Interface Board in the PC” on page 56.
• The GPIB interface was replaced by a different type or was installed in a
different slot. Rerun I/O Config, remove the configured card and
reconfigure the new one.

Figure 22 The GPIB Interface Has Already Been Configured.

118 Installing ChemStation for LC Systems

 Troubleshooting 7
Troubleshooting GPIB Interface Problems

• The interface is set up to use an I/O port address (Base Address) or

interrupt request line (IRQ) that is already being used. To solve this
problem, you need to resolve the I/O port address conflict (see “Resolving
Agilent 82341 I/O Port Conflicts” on page 119) or IRQ conflict (see
“Resolving Agilent 82341 IRQ Conflicts” on page 120). This does only apply
to the Agilent 82341 GPIB interface board as the Agilent 82350 is a PCI
board.
• The interface has already been configured by I/O Config.To solve this
problem, you need to edit the existing configuration entry for the interface,
instead of trying to add a new interface. In the main I/O Config window,
click on the name of the interface configuration entry for the Agilent 82341
in the Configured Interfaces list box. Then click on the Edit button directly
beneath the Configured Interfaces list box to edit the configuration entry
for the Agilent 82341 GPIB interface.

Resolving Agilent 82341 I/O Port Conflicts

If I/O Config does not find an Agilent 82341 GPIB interface when you try to
add a configuration entry for it, you may have an I/O port (Base Address)
conflict with that interface. Neither I/O Config nor the Agilent ChemStation
will recognize the Agilent 82341 GPIB interface if a conflict exists with the I/O
port Base Address used by the interface. To resolve this problem, select
another I/O port address for the interface by changing its DIP switch settings,
see Figure 5 on page 54.
Note that the selected I/O port address ranges must not conflict with other I/O
interfaces installed in your computer, including other manufacturer's
products (such as LAN interfaces, etc.). Refer to the documentation for the
other interfaces and the following section to select unique Base Addresses for
all the interfaces in your computer.

Installing ChemStation for LC Systems 119

7 Troubleshooting
Troubleshooting GPIB Interface Problems

Resolving Agilent 82341 IRQ Conflicts

The interrupt request line (IRQ) assigned for an Agilent 82341 GPIB interface
by I/O Config must be reserved for exclusive use by the interface. If this
interrupt line is already being used by another interface, this will cause
unpredictable behavior (such as system crashes, LAN problems, mouse
tracking problems, etc.).
If you suspect an IRQ conflict exists between an Agilent 82341 interface and
another interface on your system, edit the configuration entry for the Agilent
82341 interface and assign another IRQ for the interface.

“Autoadd Button” of IOCFG Installs Defaults Incompatible with the

Agilent ChemStation
IOCFG has an “Autoadd” button. We strongly discourage from using the
Autoadd button because it installs default settings that are not compatible
with the Agilent ChemStation. Please configure the IO Libraries interactively
as described in Chapter 5, “Checking Out the Agilent ChemStation”.

120 Installing ChemStation for LC Systems

 Troubleshooting 7
Software Start-up Problems

Software Start-up Problems

An online core must be purchased in order to install additional

instrument modules
Most probably, a previous version of the Agilent ChemStation was installed on
the PC, but the HPCHEM directory subtree was deleted before starting setup.
WIN.INI still contains references to the deleted Agilent ChemStation
installation in the [PCS] and [PCS,...] sections. Delete the [PCS] sections in
WIN.INI.

ChemStation cannot be located on your system. You must first

install Agilent ChemStation before installing an instrument
Before installing an add-on instrument control module, you need to install the
Agilent ChemStation core (e.g. G2170AA).

ChemStation online session does not finish start-up

No valid license…
Before you can use the Agilent ChemStation, you need to enter the license
registration number supplied with the registration packet delivered with your
Agilent ChemStation installation disks. You need to enter a registration
number for every instrument module that you install.
If you installed the Agilent ChemStation yourself, the installation routine
prompts you to enter the license registration number of the appropriate
instrument module.
If the Agilent ChemStation core software was preinstalled by Agilent
Technologies, you need to install the instrument module software and the
license registration number.

Installing ChemStation for LC Systems 121

7 Troubleshooting
Software Start-up Problems

If you do not specify the license registration number at time of installation,

you need to start the license registration utility from the Agilent ChemStations
group of Windows Program Manager. The license registration utility prompts
you for the appropriate license registration numbers.
The Agilent ChemStation does not initialize an instrument module for which
no valid license registration number has been entered.

Instrument not found

GPIB communication to the configured instrument or instrument module is
not possible. This may be caused because:
• the instrument is turned off,
• the instrument’s GPIB address does not match the address configured in
the Agilent ChemStation,
• the GPIB cable is not properly connected,
• the GPIB board was not correctly configured (please refer to “Configuring
the Agilent 82341 or Agilent 82350 GPIB Interface Drivers” on page 58) or
• the GPIB card is incompatible with your PC.

System Status shows Not Ready

The cause for being in a not-ready state is detected by the instrument.
Not-ready conditions of the Agilent liquid chromatographs are described in
the documentation accompanying the respective instrument.

Cannot find HPNLS01.DLL

Windows needs this file to run C:\HPCHEM\CORE\HPCORE.EXE
The Agilent ChemStation does not start. You receive error messages that
indicate that the Agilent ChemStation initialization could not access specific
libraries. You can close all of the error message boxes.
Verify that the Agilent ChemStation main directories (default: C:\HPCHEM
and C:\HPCHEM\SYS) are included in the PATH setting of the PC by typing
PATH at the MS-DOS prompt. If the Agilent ChemStation system directories
are listed in the PATH setting, simply close all applications and restart the
computer.

122 Installing ChemStation for LC Systems

 Troubleshooting 7
Software Start-up Problems

If the directories are not part of the PATH setting, open C:\AUTOEXEC.BAT,
find the last entry that specifies “SET PATH” and append “C:\HPCHEM;C:\
HPCHEM\SYS” to the line.

…key in section [PCS…] of WIN.INI not valid or specified file does

not exist
File Initialization failed
The Agilent ChemStation reports an error during the initialization of its file
structure. Verify that the Agilent ChemStation data, method and sequence
path settings all point to existing directories on your PC’s hard disk. You can
view these settings in the configuration editor.
Go to the [PCS…] section in WIN.INI that was specified in the error message
(for example, [PCS,1] and check the contents of the key entry.
If the mismatch is in the _Exe(cution)$ key, check whether the _EXEPATH$
key contains the correct path specification for the Agilent ChemStation core
modules (default: C:\HPCHEM\CORE\).
If the _Meth(od)File$ key is reported to be inconsistent, verify that your
instrument method directory (for example, C:\HPCHEM\1\METHOD)
contains a method directory called def_lc.m.
If the _Seq(uence)File$ key is reported to be inconsistent, verify that your
instrument sequence directory (for example, C:\HPCHEM\1\SEQUENCE)
contains a sequence file called def_lc.s.
If one or both of them do not exist, either restore a backup copy, copy the
corresponding files and directories from another Agilent ChemStation
instrument or reinstall the software.

System Exception in dialogs.c

The Agilent ChemStation was terminated abnormally and does not restart.
This is usually caused by specific libraries of the application still being active
in the PC’s memory. Close down all applications and restart Windows.

Installing ChemStation for LC Systems 123

7 Troubleshooting
Software Start-up Problems

Configuration Editor no longer runs

If changes are made to the Windows WIN.INI configuration file manually, or
WIN.INI becomes corrupted, it is possible that syntax errors or
inconsistencies are introduced into the Agilent ChemStation sections of this
file which cause the Configuration Editor to fail.
The best solution to this problem is to restore a backup copy of the WIN.INI
file which does not have the errors. If a backup is not available, the
configuration sections can be removed manually and the configuration
restored using the Configuration Editor. To do this, edit the WIN.INI file using
the NOTEPAD editor and remove the sections that are preceded by [PCS,1],
[PCS,2], [PCS,3] and [PCS,4], including the section titles. Then, edit the lines
for devices and instruments in the main [PCS] section to read:
devices=
instruments=
Remove all of the other device statements. At this point, you should be able to
run the Configuration Editor. Add the instruments back to the configuration
and reconfigure the instrument devices.
If the configuration information is still inconsistent and the configuration
editor does not open, we recommend that you remove the [PCS] section from
WIN.INI and re-install the software.

Autostart Macro failed

During the initialization, the Agilent ChemStation automatically loads and
executes macro code from a defined set of macro files. A run time error caused
the autostart macro to fail.
A text error message is displayed in the (red) message line of the
Agilent ChemStation.
If you have added your own customization macro code to User.Mac in the
Agilent ChemStation core directory (default path: C:\HPCHEM\CORE), check
that all the macros loaded in this macro file are correctly specified.
If you cannot identify or isolate the problem, rename User.Mac to Usr.Mac and
restart the Agilent ChemStation. If the error message does not occur, you need
to debug your customization code.

124 Installing ChemStation for LC Systems

 Troubleshooting 7
Software Start-up Problems

If the error persists, the working copy of the Agilent ChemStation’s

configuration register may have been corrupted. Rename or delete the
configuration register in the appropriate instrument directory:
• the configuration register for instrument 1 online is C:\HPCHEM\1\
CONFIG.REG, or
• the configuration register for instrument 1 off-line is C:\HPCHEM\1\
CONF_OFF.REG.
Alternatively, one of the Agilent ChemStation’s macro files may have been
modified or corrupted. In this case, you need to reinstall the
Agilent ChemStation software.

General Protection Fault in Module...

The operating system reports a General Protection Fault (GPF) when it detects
that an application has attempted write access to a memory location that
belongs to another application or process. Potentially, a GPF is the
consequence of a system corruption. For the diagnosis of GPFs, it is
mandatory to record the exact error message and error address information in
order to identify the part of an application program that caused the failure.
Windows allows tracking of GPFs with a utility called Dr. Watson. For more
information on this utility, please refer to “Using the DrWatson Utility” on
page 143.

Installing ChemStation for LC Systems 125

7 Troubleshooting
Printing Problems

Printing Problems

ChemStation Print Spooler hangs after an error occurred

If the Agilent ChemStation spooler does not continue after a print error has
occurred, try initializing the Agilent ChemStation spooler by typing the
following command on the Agilent ChemStation command line:
_LoadServiceResetPrinting
the Agilent ChemStation spooler prompts you whether to cancel all pending
print jobs. If you do not want to cancel the pending jobs, press No. If the
printing error comes again, you need to save your work, close all applications
and restart Windows and the Agilent ChemStation to reinitialize the operating
environment.

Printing in Multi-Instrument configurations

When printing simultaneously from multiple Agilent ChemStation
instruments (e.g during a sequence), resource conflicts may result in printing
errors.
If you experience printing problems that appear to be related to a temporary
shortage of available system resources as multiple programs print in parallel,
you can reduce the Agilent ChemStation’s frequency to hand back CPU control
to another application by typing the following command on the
Agilent ChemStation command line:
_LoadServiceChromSplYield 2000
This command specifies how often (in milliseconds) the Agilent ChemStation
Spooler lets other application use the computer’s CPU. The default value is
300 ms. Increasing this number increases the printing speed at the expense of
user interface response and should only be used in automated mode.
You can make this setting permanent by adding it to a macro file called
USER.MAC in the Agilent ChemStation core directory (default: C:\HPCHEM\
CORE). This executes the command automatically every time the

126 Installing ChemStation for LC Systems

 Troubleshooting 7
Printing Problems

Agilent ChemStation is started. For further details on the customization

possibilities associated with USER.MAC, please refer to the Macro
Programming Guide which is available as online help.

Recovering from Printer hang-ups

If for some reason the communication to the printer hangs and the printer
therefore does not finish the current printout, there are two steps that have to
be done:
Depending on whether the printer is local at your PC or connected via a
network:
• on a local printer do a reset on the printer panel itself, or
• on a networked printer you have to clear the network communication
problem. This may involve checking cabling, or checking the printer spooler
on the network host machine, or doing a reset on the printer panel itself.
On your PC the printer driver or the Windows Print Manager has to be reset.
This is done by one of these alternatives:
• if there is a printer driver dialog box visible that has just a Printing… or
similar message in it together with a Cancel button, press that Cancel
button, or
• if instead there is the icon of the Windows Print Manager visible, close it,
discarding the actual print jobs.
If the problem persists try stopping and restarting the Windows Spooler
Service. Select Services from the Windows Control Panel, scroll down the list
and highlight the item “Spooler”. Choose “Stop” to stop the service and restart
it again selecting “Start”.
When the Agilent ChemStation spooler reports printer errors, a message
window displays the error messages, for example:
Printing problem 106, with page file:
c:\hpchem\1\temp\~p3d0004.tmp
These failed print files can be printed with a command typed on the command
line, for example
MFPrint "c:\hpchem\1\temp\~p3d0004.tmp"
Do not forget to delete these temporary files after you have printed them.

Installing ChemStation for LC Systems 127

7 Troubleshooting
Printing Problems

Printing Messages
Printing Problem 100
A file belonging to the current print job could not be found. Check the
consistency of your hard disk’s file structure.

Printing Problems 101, 102, 106, 108, 110, 210, 212, 300
Due to low system resources or not enough available disk space, a print-file
could not be accessed either in memory or on disk.
Check free system resources using the Windows Task Manager. Press
“Ctrl + Alt + Del” and select “Task Manager”. On the Performance Tab you will
find the available physical memory. If free system resources are significantly
below 30 %, you should save your work, close all Windows applications and
restart Windows.

Printing Problem 104

The print page could not be copied in memory. Check available memory on
your computer.

Printing Problem 202

The printer driver could not be initialized.
Reset the Agilent ChemStation spooler and check the printer driver name and
version of your printer. Refer to the list of tested printers on the Agilent
ChemStation Software CD-ROM.
Check available system resources.

Printing Problem 204

A print page could not be sent to the printer driver.
Verify that your printer is correctly configured and connected and online.

Printing Problems 206, 208, 302

Printer escape sequences to initialize a new page or indicate the end of a print
job could not be sent to the printer driver.

128 Installing ChemStation for LC Systems

 Troubleshooting 7
Printing Problems

Verify that your printer is correctly configured, connected and online.

Printing Problem 214

A print job could not be removed from the print queue. Either the print job file
does not exist in the temporary directory or the Agilent ChemStation print
queue file (hpspl100.que) does not exist.

The print queue file and Agilent ChemStation temporary files must not be deleted while
CAUTION
the Agilent ChemStation is running.

Printing Problem 400, 401, 402, 403

The Agilent ChemStation spooler could not be initialized properly. If this
occurs as a consequence of previous errors, save your work, terminate all your
applications and restart Windows.

Parts of the chromatogram missing on the report or strange fonts in the report
This may be caused by low system resources in Windows. Either too many
applications are running or one or more applications have not freed up all the
system resources that they allocated during their operation. Check the
percentage free of system resources in the Windows Task Manager. When free
system resources get below 30 %, you should save your work, close all
applications and restart Windows.

Installing ChemStation for LC Systems 129

7 Troubleshooting
Computer Problems

Computer Problems

Sporadic Hang-Ups
Use CHKDSK (see Chapter 8, “Maintaining the Agilent ChemStation
Computer”) to verify that there are no inconsistencies in the file structure. If
you find such inconsistencies on your PC’s hard disk, correct them (see
“Scanning and Repairing NTFS Volumes” on page 137).

System Crash in an Online Session

If your system crashes when trying to establish the GPIB communication to
the chromatograph, there may be hardware conflict between the GPIB board
and another device installed in your PC (for example, a specific graphics
accelerator card, an infrared port, a soundcard). Using a different IO port
and/or a different interrupt level for the GPIB card can eliminate the problem.
Refer to the GPIB card configuration information in this handbook.

Cannot create file…

Check the available disk space on your system. Delete unnecessary files, for
example, left over temporary files (see Chapter 8, “Maintaining the Agilent
ChemStation Computer”) or archive data files that you do not currently need
to a backup medium (see “Performing Backups” on page 139).

Slow Hard-Disk access or Hard-Disk activity LED is continuously

flickering
Your hard disk may be fragmented. Use a defragmentation utility to reorganize
the logical order of file clusters on your disk (see “Defragmenting an NTFS
Volume” on page 137). If system performance is generally low and your

130 Installing ChemStation for LC Systems

 Troubleshooting 7
Computer Problems

hard-disk appears to be accessed very frequently for relatively long periods of

time, the system is most probably low on RAM and using virtual memory (i.e.
the page file) excessively.
• Reduce the number of concurrent programs.
• Use Computer Management options (e.g. 2000: right-click on My computer
icon and select Manage) to access the disk management utility.
• Check whether your disk cache utility is installed and optimally configured.

Agilent ChemStation performance degrades over time

If your environment requires continuous routine operation of the Agilent
ChemStation over days or even weeks without restarting Windows, the system
performance may degrade over time due to memory and resource leaks in the
system. To overcome this problem, we recommend rebooting the PC on a
regular basis.Windows Service Packs are available on the Microsoft Home
page.

Installing ChemStation for LC Systems 131

7 Troubleshooting
LC Instrument Start-up Problems

LC Instrument Start-up Problems

Agilent ChemStation does not see an LC hardware modification (HP 1050 or HP

1090)
After modifying the hardware configuration of the LC or an LC module, this
change may not be reflected by the Agilent ChemStation.
For example, the Agilent ChemStation does not see the 34-vial tray on the
HP 1050 autosampler after the 21 vial tray has been used.
All of these changes require you to close the Agilent ChemStation down and
reinitialize it. Subconfiguration information of the LC hardware is only
checked at start-up time of the Agilent ChemStation.
Implementing the modification while the Agilent ChemStation is running and
simply resetting the module from the Agilent ChemStation user interface is
not sufficient.

System remains in a wait-state waiting for a module that has been taken out of
the configuration
On systems that are frequently reconfigured, i.e. devices are added or removed
from the current configuration, the system may remain in a wait-state as long
as the unused devices are still connected to the APG remote cable. This occurs
typically when the HP 1040 DAD is removed from the current configuration,
but remains connected.
The only possible work-around is to disconnect/connect the remote cable
when deleting/adding the HP 1040 DAD from/to the current configuration.
If a second detector is not needed for certain experiments, but for convenience
reasons is not taken out of the configuration, selecting a very short run-time
for the unneeded detector is not recommended.
For example, when acquiring data on an FLD while the DAD is configured
with a short stop-time, it may happen that the DAD lamp is switched off before
the LC analysis is finished. This produces a not ready condition so that the
next analysis does not start. Alternatively, acquiring with the DAD and
selecting a short stop time for the FLD does not produce this problem as a
lamp off status is a perfectly valid operational mode with the FLD.

132 Installing ChemStation for LC Systems

 Troubleshooting 7
LC Instrument Start-up Problems

Data File is empty (contains no signal)

Check the remote cabling of the detectors. A detector does not change into the
run-status unless the start signal is transmitted over the remote line. Refer to
the cabling diagrams in “Installing the Analytical Instruments” on page 65.

Devices report “Buffer Overflow” in the logbook

This messages usually indicates by a bad performing PC, when the data
transfer from the instrument to the PC is not capable of handling the data
stream. The bad performance can be cause by
• Power save features of the PC, refer to section “Advanced Power
Management Not Supported with Analytical Hardware” on page 45.
• Bad performing network, refer to section “Frequent Buffer Overrun in
Instrument Log Book” on page 117.
• Bad performing hard disk, see section “Slow Hard-Disk access or Hard-Disk
activity LED is continuously flickering” on page 130.
• Other programs accessing the hard disk or using the computers resources,
like schedule defragmentation, back up or virus scanning.

Agilent 1100 System with Agilent G1315A DAD hangs during initialization
Initialization problems have been reported with some early Agilent 1100 Diode
Array Detectors running firmware revision A.02.03 and before. Sporadically,
the system would not finish the boot-up sequence, requiring the user to power
down the instrument as well as the PC.
To overcome this problem, the Agilent 1100 firmware should be updated.

Installing ChemStation for LC Systems 133

7 Troubleshooting
LC Instrument Start-up Problems

134 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

8
Maintaining the Agilent ChemStation
Computer
Cleaning up Left-Over Temporary Files 136
Maintaining the PC’s File System 137
Performing Backups 139
Gathering System Information 142
Using the DrWatson Utility 143

Agilent Technologies 135

8 Maintaining the Agilent ChemStation Computer
Cleaning up Left-Over Temporary Files

Cleaning up Left-Over Temporary Files

Occasionally, temporary files may accumulate in the directory specified by the

TEMP environment variable. These files generally are left open when Windows
is abnormally terminated, for example, by powering your computer down
without closing Windows first. The Windows temporary files are named
~XXXXXXX.TMP, where XXXXXXX are characters and numbers produced by
the program that created the temporary file. To recover the temporary space,
you should delete these files after closing all currently running applications.
To determine which directory is currently being used for temporary files, type
SET at the command prompt. This will echo back the current settings for all
user and system environmental variables.

Notes on the Usage of Long File Names

Current Windows operating systems support long file names, i.e. file names
that consist of more characters than in the legacy 8.3 file name convention (8
characters for the prefix and 3 characters for the extension). The
Agilent ChemStation currently does not support long file names.
The FAT file system has a hard-coded limit of 512 root level entries and every
long file name occupies multiple directory entries. Consequently, the user can
run out of directory entries depending on the number and the file name length
of entries contained in the root directory of a FAT volume. This is one reason
that Agilent recommend the NTFS file system be used for all Agilent
ChemStation systems.

136 Installing ChemStation for LC Systems

 Maintaining the Agilent ChemStation Computer 8
Maintaining the PC’s File System

Maintaining the PC’s File System

Scanning and Repairing NTFS Volumes

Use the Windows utility CHKDSK from the command prompt to scan and
repair NTFS volumes. This program incorporates all of the functionality of the
MS DOS-based Chkdsk and Scandisk utilities, including a surface scan. To do a
surface scan, use CHKDSK /R. The disk check can also be performed by
accessing a disk drive’s properties window and selecting the tools tab.
Windows 2000 and Windows XP Professional perform an autocheck routine
upon every boot. If this routine detects a consistency error on the volume, it
automatically runs the CHKDSK /F command to fix it. If Windows is unable to
run the CHKDSK /F command (for example, because you want to run it on
the boot partition or because someone is accessing the partition from the
network), this task is postponed until the next time the system reboots.

Defragmenting an NTFS Volume

Compared to the FAT file system, fragmentation of files on an NTFS volume is
greatly reduced. By design, fragmentation only occurs on an NTFS volume if a
file increases in size after it is on the drive. This means that backing up a
volume to tape and restoring it from tape will result in an unfragmented
volume.
Windows 2000 Professional and Windows XP Professional does include a
simple defragmentation utility. Third-party programs are available that can
defragment both Windows 2000 and Windows XP volumes. Agilent
recommend Diskeeper from Executive Software (http://www.execsoft.com/).

Do not defragement your hard disk drive during data acquisition.

CAUTION

Installing ChemStation for LC Systems 137

8 Maintaining the Agilent ChemStation Computer
Maintaining the PC’s File System

Scanning for Viruses

If the Agilent ChemStation will communicate with other systems (LAN,
modem, BBS, etc.) or if you share files with other computer users, we
recommend scanning the drives for computer viruses. Many different utilities
are available for virus scanning. Many of these utilities also come with
periodic updates as new viruses are discovered.
If you download programs from the internet or exchange documents or
programs through e-mail or flexible disks, a virus-check utility is mandatory.
Agilent ChemStation disks (flexible disks, hard-disks and CD-ROMs) are
consistently checked for known viruses.

Never perform a virus scan during data acquisition, as this will degrade the performance of
NOTE the hard disk and the acquired data might not be written to the hard disk fast enough,
causing a power fail or buffer overrun message in the instrument log book and possible loss
of data.

138 Installing ChemStation for LC Systems

 Maintaining the Agilent ChemStation Computer 8
Performing Backups

Performing Backups

In order to protect your valuable data, an appropriate backup strategy is

mandatory. We recommend to archive your data files in regular intervals. If
your data is no longer required to be available online, you should also archive
them and remove them from your hard disk. This will free up valuable storage
space on your computer’s hard disk as data acquired from a diode array
detector may easily exceed several megabytes in size.

Windows 2000/XP Backup

Windows 2000 and Windows XP Professional both include compatible backup
programs that support many standard tape devices. The backup utility also
allows for backing up to a single file, which can then be placed on an offline
medium. Both backup utilities allow the Windows Registry to be backed up as
well as data.

Automating and Scheduling Windows 2000/XP Backup

Windows 2000/XP allows automation and scheduling of Backup jobs through
the use of batch files and the Scheduler service. The Scheduler is available by
default for Windows 2000 and Windows XP.

Never perform a back up during data acquisition, as this will degrade the performance of
NOTE the hard disk and the acquired data might not be written to the hard disk fast enough,
causing a power fail or buffer overrun message in the instrument log book and possible loss
of data.

Installing ChemStation for LC Systems 139

8 Maintaining the Agilent ChemStation Computer
Performing Backups

Travan Tape Drives

Tape drives are the most common backup devices available today. The IDE
tape drives combine lowest initial hardware cost with acceptable performance
(up to 4 GB/hour). Travan tapes are a reasonable backup solution for most
laboratories that run their Agilent ChemStation on single workstation PCs.
The minimum storage capacity per tape is currently around 2.5 GB of
uncompressed data.
You will need an appropriate backup utility that supports the drive.

Never run an MS-DOS-based tape backup utility from Windows. This may result in corrupt
NOTE file information on the tape.

Digital Audio Tape (DAT) Drives

Digital audio tape (DAT) tape drives are suited to handle large amounts of
data (storage capacity is typically 2 to 6 GB or more), but do require a SCSI
interface installed in the PC.

Magneto-Optical (MO) Disk Drives

MO disk drives are high storage capacity devices (at least 1.3 GB) connected
externally to a SCSI interface that must be installed in your PC. MO disk
drives are capable of reading and writing write-once media and rewritable
disks.
No specific backup software is required. The supporting drivers are shipped
with the operating system or the device and allow for accessing the additional
MO disk as if it was a floppy disk.

If the rewritable disk is formatted with NTFS, it cannot be “hot-swapped”, i.e. ejected and
NOTE exchanged while the system is running. To change disks, you need to shut down Windows.

140 Installing ChemStation for LC Systems

 Maintaining the Agilent ChemStation Computer 8
Performing Backups

Recordable CD-ROM
CD-R (CD-Recordable) systems allow you to record up to 650 MB of data on a
single CD-R disk. The CD reader/recorder is usually connected to your PC the
IDE interface. The CD-R media have a very long life, making them ideally
suited to archiving. The CD-R disks can be read by other computers fitted with
CD-ROM drives and appropriate software.

Never perform a backup with a Windows-based backup utility while the

CAUTION
Agilent ChemStation is acquiring data. This may result in inconsistencies in the file
structure stored on the backup medium.

Network Servers
Network servers can also be used for data backup purposes, provided that the
network system administrator has implemented an appropriate backup
strategy for data stored on the server’s disk. One strategy is to allocate a
portion of the server’s hard disk space for your Agilent ChemStation. This
portion on the server is called a share which you can use for your standard file
operations (access, copy, evaluate files) within Windows.
The advantage of this approach is that backup will be performed centrally by a
system administrator for all users that have accounts (logon capabilities) on
the system.

Using a 3.5-inch floppy disk drive is not a reasonable backup solution for the
NOTE Agilent ChemStation.

Installing ChemStation for LC Systems 141

8 Maintaining the Agilent ChemStation Computer
Gathering System Information

Gathering System Information

Gathering System Information with Windows 2000/XP

Windows 2000 and Windows XP Professional include a comprehensive
information reporting and diagnostics program that collects and presents
information on device driver information, network usage and system
resources such as IRQ, DMA and IO addresses. This utility is called “System
Information” and can be found under the System Tools menu in the Windows
Accessories menu.
Hardware conflicts will usually also be logged in the Windows Event Viewer, if
for instance, Windows cannot start a service due to a misconfigured device.

For Windows XP Professional Error Reporting is implemented and enabled by

default.
To access the settings for the reporting feature:
1 Click Start
2 Right-click My Computer, then click Properties
3 Click on the Advanced tab
4 Click Error Reporting
For more information, refer to Windows XP Professional help.

142 Installing ChemStation for LC Systems

 Maintaining the Agilent ChemStation Computer 8
Using the DrWatson Utility

Using the DrWatson Utility

Who Is Dr. Watson?

Dr. Watson (“DrWatson.EXE”) is a Microsoft diagnostic tool that provides
detailed information on the internal state of Windows when General
Protection Faults (GPF) occur on the system.
There is a version designed for Windows 3.1, Windows for Workgroups 3.11
and Windows 95 and one for Windows NT. If an application error occurs, Dr.
Watson automatically creates a special log file in your WINDOWS directory
named DRWATSON.LOG. Dr. Watson also prompts for details about the
circumstances (scenario) under which the application error occurred.
Sometimes Dr. Watson detects a fault that might not be fatal, and you are given
the opportunity to ignore the fault or close the application.
When you choose the Ignore option after an application error is detected,
Windows for Workgroups continues without performing the faulting
instruction. You might be able to save your work in a new file at this point, but
do not continue using the application for other actions. We recommend to quit
Windows after saving your work and then restart the PC.
Ignoring the fault may lead to unpredictable results as the first fault already
indicated a potentially severe corruption of the system.
When you choose Close, the General Protection Fault dialog box appears, with
data that is useful in tracking the source of the error. This information is saved
in the DRWATSON.LOG file.
If General Protection Faults reoccur sporadically or even regularly, supply
DRWATSON.LOG to your application vendor to discuss the source of the
application error.

Installing ChemStation for LC Systems 143

8 Maintaining the Agilent ChemStation Computer
Using the DrWatson Utility

Dr. Watson in Windows 2000 /XP

Windows NT, Windows 2000 and Windows XP Professional are coming with
two versions of the Dr. Watson utility. There is a 32-bit version of Dr. Watson
that traps General Protection Faults caused by 32-bit applications.
DRWATSN32.EXE is enabled by default. Errors are logged in a file called
DRWATSN32.LOG. More details can be found in the Windows help system.
The second utility is a 16-bit version of Dr. Watson. To install it, create a
shortcut to DRWATSON.EXE in the StartUp group. General Protection Faults
that occur in 16-bit applications are automatically logged in DRWATSON.LOG.
Unfortunately, this version of DrWatson does not decode segment offsets. This
information is useful to the software engineers to identify the source of a
failure. For detailed troubleshooting, we recommend replacing current version
of DrWatson with version 0.80 that was included with Windows 3.1.
The level of detail of the DrWatson failure reports can be controlled by entries
in WIN.INI.
Example:
[Dr. Watson]
ShowInfo=dis loc mod par
TrapZero=1 ; trap floating point exceptions
DisStack=16 ; 16 stack levels to be disassembled
DisLen=32 ; 32 instructions disassembled in stack traces
This enables disassembly of the fault address, stack trace disassembly
including local variable and parameter values, the list of loaded modules and
DLLs and trapping of floating point exceptions.

144 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

9
Upgrading From a Previous Revision of
ChemStation
Introduction 146
Importing Files from a Previous ChemStation Revision 147
Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series) 151
Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series) 153
Upgrading from A.02.0x of the HPLC ChemStation (DOS Series) 154
Upgrading from A.03.0x of the ChemStation 155
Upgrading from A.04.0x of the ChemStation 159
Upgrading from A.05.0x of the ChemStation 161
Upgrading from A.06.0x of the ChemStation 162
Upgrading from A.07.0x of the ChemStation 163
Upgrading from A.08.0x of the ChemStation 164
Upgrading from A.09.0x of the ChemStation 165
Importing HPLC ChemStation (Pascal Series) Files 167
Importing Pascal ChemStation files using the LIF Utility 168

Agilent Technologies 145

9 Upgrading From a Previous Revision of ChemStation
Introduction

Introduction

The installation program allows you to upgrade your system from a

previously-installed ChemStation.
The new version installs over an existing installation. Existing sequence,
method, data and user files are maintained as your original configuration
(instruments, attached devices, addresses).
The ChemStation’s binary files (executable files identified by the extension
.exe and .dll) and the ChemStation’s macro files (extension .mac and .mcx)
install over the existing files.
To save disk space, the contents of the LCEXE (LC3DEXE, CE3DEXE)
directories are deleted during the upgrade.

Co-residence (parallel installations) and co-execution (running concurrently) of the old and
NOTE new ChemStation revisions are not supported.

Files that you added to customize your previous revision of the ChemStation
are saved in the ChemStation backup folder (default: C:\HPCHEM\BACKUP).
This includes the USER.MAC macro file, if it exists on your system.
ChemStation customization code must be reinstalled after an upgrade and
checked for compatibility.
The existing icons in the ChemStations group are replaced with the ones
pointing to the new installation.

There is no automatic upgrade procedure if your previous ChemStation was the HPLC
NOTE ChemStation (Pascal Series).

146 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Importing Files from a Previous ChemStation Revision

Importing Files from a Previous ChemStation Revision

The conventions for the sequence and method files have been adapted to the
new features that have been implemented since the first introduction. The
upgrade path that needs to be followed to convert your analytical sequences
and methods depends on the revision of the ChemStation you are upgrading.
In all cases, the raw data file structure has not been changed since the first
introduction of the HPLC ChemStation (DOS Series) in 1990, so you can
directly load your data.

Importing Sequence Files from Revisions Prior to A.03.01

The sequencing capabilities of this version of the ChemStation include partial
sequencing and user-defined data file naming which were not available in the
previous revisions. The sequence file structure has changed from a
human-readable (and thus modifiable) format to a binary format.
The structure of the sequence tables has been simplified by eliminating
redundant tables (the sample log table and the sequence table are now
combined into one). This has an impact on the data file naming conventions of
sequence data files as the sequence structure information is coded into the
data file names of the signals acquired during a sequence.

Reprocessing Sequence Data Imported from a Previous Revision

The steps required to convert a sequence defined with a previous revision of
the HPLC2D or HPLC3D ChemStation (DOS Series) are:
• Open the Method&Run Control view.
• Load the appropriate sequence file using the File-Load-Sequence menu. The
ChemStation loads the old sequence file and internally flags that the
current sequence is an old one.
• Set the Sequence Parameters accordingly (e.g. switch to the appropriate
data file directory, switch to Reprocessing Only mode).

Installing ChemStation for LC Systems 147

9 Upgrading From a Previous Revision of ChemStation
Importing Files from a Previous ChemStation Revision

• Start the sequence by specifying Run Sequence from the Method&Run

Control Viewer by simply pressing the F6 key.
• Save the sequence using a different name using the File-Save As-Sequence
menu.

Do not overwrite your original sequence file if you need to be able to rerun the sequence on
NOTE a previous revision of the ChemStation (e.g. for validation purposes) or need to reprocess
sequence data that you imported from a previous ChemStation revision.

If you make changes to the sequence table, your sequence is saved according to
the new structure. If your original table contains lines that specify runs on a
range of vials (e.g. Line 1 run method PEST.M from vial 10 to vial 12 with 2
injections per vial), reprocessing the imported sequence data files does not
work. Of course, reacquiring and processing your sequence data works
because the sequence data are stored according to the new structure.
The tables below illustrates the difference in sequence processing between the
two versions.

Table 17 Old Sequence Table

Sequence line Method From Vial To Vial Injections per Vial

1 Mth1 10 12 2

Table 18 Old Sequence Data File Names

Data File Name Sequence Line Vial Number Injection Number

010-0101.d 1 10 1

010-0102.d 1 10 2

011-0101.d 1 11 1

011-0102.d 1 11 2

012-0101.d 1 12 1

012-0102.d 1 12 2

148 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Importing Files from a Previous ChemStation Revision

Table 19 New Sequence Data File Names

Data File Name Sequence Line Vial Number Injection Number

010-0101.d 1 10 1

010-0102.d 1 10 2

011-0201.d 2 11 1

011-0202.d 2 11 2

012-0301.d 3 12 1

012-0302.d 3 12 2

Table 20 New Sequence Table (After Conversion to New Sequence Structure)

Sequence Line Method Vial Injections per Vial

1 Mth1 10 2

2 Mth1 11 2

3 Mth1 12 2

Importing Data Files

Data acquired on previous versions of the HPLC ChemStation (DOS Series)
can be imported directly (no conversion necessary).

Installing ChemStation for LC Systems 149

9 Upgrading From a Previous Revision of ChemStation
Importing Files from a Previous ChemStation Revision

Importing Customized Report Templates

Although the ChemStation now provides a new easy-to-use customized report
generator, customized report templates generated with the customized report
layout designer of rev. A.01.00 and A.02.0x of the HPLC ChemStation (DOS
Series) can still be used.
If you wish to import a user report template created with a previous version of
the ChemStation, you need to perform the steps according to the example
outlined below.
For example, assume you want to add your customized report template called
aa.tpr to the list of available report styles. The title you assign to the
customized report is Amino Acids.
1 Copy your user report template (e.g. aa.tpr) to the ChemStation core
directory (default: C:\HPCHEM\CORE).
2 On the ChemStation command line, type:
AddUserReportStyle “Amino Acids”,”Customize ,””aa””
This adds your template (aa.tpr) to the list of available user report styles. The
name of the report style is displayed as Amino Acids. Please note the space
after the Customize command and the number of quotes that are necessary for
syntactical reasons.

Modifying imported report templates using the new customized report generator is
WA R N I N G
not possible. To do this, you have to rebuild your report template using the new tool.
Please refer to the online help information for more details on how to set up a
customized report template.

Delete a User Report Style

You can delete a user report style from the list of available report styles.
For example, assume you want to delete the report style named Amino Acids
from the list of available reports.
Type the following command on the ChemStation command line:
DeleteUserReportStyle “Amino Acids”
This removes a report style entitled Amino Acids from the list of available
report styles.

150 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series)

Upgrading from A.01.0x of the HPLC2D ChemStation (DOS

Series)

The HPLC2D ChemStation was designed to operate with MS-DOS 3.3 or greater
and Windows 3.0.

Importing Method Files

A ChemStation method defines the parameters for instrument control and
data acquisition, data analysis and contains recalibration definitions.
In revision A.01.0x of the HPLC2D ChemStation (DOS Series), the data
analysis part of the method was implemented as an ChemStation macro. In
the later revisions, this was modified so all data analysis parameters were
stored in a binary register file.
To import a method generated with revision A.01.0x of the HPLC2D
ChemStation (DOS Series), do the following:
1 Switch to the Data Analysis View.
2 Load the default method called def_lc.m by using the File-Load-Method
menu. Doing this, a default binary data analysis method register is loaded
from disk.
3 Load a data file that was acquired with the method you want to import by
using the File-Load Signal menu. This adds the appropriate signal
description of the signal to be evaluated by the method to the list of signals.
4 Load the method file that you want to convert using the File-Load Method
menu.

Installing ChemStation for LC Systems 151

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series)

5 Save the method under a different name using the File-Save As-Method
menu.

Do not overwrite your original method if you need to be able to rerun the method using a
NOTE previous revision of the ChemStation (e.g. for validation purposes).

Your entire method, including e.g. the calibration table, is now converted to
the new revision.

Method Changes since A.01.0x

• A.01.0x includes an older version of the Integrator module. The values
accepted for the threshold settings have been redefined. To make the
integration threshold settings equivalent on both platforms, the threshold
values need to be increased by 5. For instance, if the original A.01.0x
method specifies an initial threshold value of -7, you need to modify this
setting to -2 in order for the integrator to produce equivalent results.
• The conventions followed by G1304/5A (HPLC2D ChemStation), revision
A.01.0x, regarding the origin handling in calibration curve calculations are
different from the conventions followed in the later revisions. Revision
A.01.0x offers three different modes for origin handling (ignore, include,
force origin). The definition of Force Origin has been changed (see below). A
new origin handling method Connect Origin has been added, which does
exactly what Force Origin did in A.01.0x.

According to the old convention, Force Origin calculates the regression curve using the
NOTE calibration points. The first calibration point is then connected to the origin. This behavior is
now called Connect Origin.

According to the new convention, Force Origin sets the offset term in the equation
NOTE describing the curve type to zero.

For exponential and logarithmic curves, the Force Origin cannot be applied as the power
NOTE curve does not have zero offset.

152 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series)

Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series)

Importing Method Files

A.01.00 of the HPLC3D ChemStation methods are directly compatible with this
version of the ChemStation.

Method Changes since A.01.00

In some cases, Qualifier Evaluation does not work after converting the method
to the new revision. This happens only if the original method was saved with
the qualifier evaluation flag set to off. As Data Analysis no longer provides a
checkbox to switch qualifiers on/off in the calibration settings dialog, the only
way to activate the use of qualifiers in the imported method is by typing the
following command on the ChemStation’s command line (refer to the
Understanding your ChemStation handbook):
SetTabHdrVal _DaMethod[1],QuantParm, UseQualifiers, 1

Installing ChemStation for LC Systems 153

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.02.0x of the HPLC ChemStation (DOS Series)

Upgrading from A.02.0x of the HPLC ChemStation (DOS Series)

Importing Method Files

Methods generated with this version of the ChemStation are directly
compatible.

Method Changes since A.02.0x

Methods now include a dilution factor, in addition to the multiplier that was
already part of revision A.02.xx methods.
Column information is restored when the new software is started for the first
time. You have to select the currently installed column from the column
information screen to make this information available on the reports. Column
information is only restored for columns that have been used and identified
for the current system. Column information from methods transferred from
other systems is not restored.

154 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.03.0x of the ChemStation

Upgrading from A.03.0x of the ChemStation

A.06.01 of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the History directory on the Supplemental Disk to
help you to determine if you need to revalidate your system.
The following areas have been enhanced in the ChemStation version A.06.01
and have a potential backward compatibility impact on upgraded installations.

Enhanced Integrator
The ChemStation supports the enhanced integrator since revision A:04.01.
Using the enhanced integrator is optional. Methods imported from a previous
version of the ChemStation can continue to use the original integration
algorithm.
The user interface allows the conversion of a method imported from a
previous version of the ChemStation. Once a method has been converted to
use the new enhanced integration algorithm, it cannot be converted back.
To evaluate the enhanced integration algorithm of the ChemStation, we
recommend that you create a backup copy of your original method.
The parameter sets of the original ChemStation integrator and the enhanced
integrator are different. For example, parameters such as the initial threshold
are values to the power of 2 of a detector specific constant.
The enhanced integrator uses the true physical dimensions for integration
parameter settings like slope sensitivity (Response/Time) or height reject
(Response).
The results calculated by the two integrators may vary on real chromatograms
due to the differences in the determination of baselines, shoulders and
tangent skimmed peaks.

Installing ChemStation for LC Systems 155

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.03.0x of the ChemStation

Internal Storage Format of Quantification Data

Revisions prior to A.04.01 used single precision floating point representation
for the internal storage of quantification data; the internal precision of the
quantification results used 7 digits.
In revision A.04.01, the internal storage format has been changed to double
precision floating point representation, and the internal precision of
quantification calculations and results is now 15 digits.
• When comparing ChemStation results to results generated on a previous
version of the ChemStation, minor discrepancies may be observed in the
last two decimals as rounding effects are now different because of the
increased precision of the internal data.
• As a consequence of the increased precision of the internal quantification
data, system verification tests generated on a previous version of the
ChemStation may now fail due to very minor differences in the number
format. If the differences of the system verification test results are caused
by the modification of the storage formats, the verification test report
states this.

Upgrading from A.03.0x Sequencing User Interface

The modifications introduced in version A.04.01 of the ChemStation are
transparent to the user but may have implications for customized systems. The
additions and enhancements necessitated to changes to the internal data
structures of the sequence engine and the ChemStation method.
Sequences created on previous revisions of the ChemStation can still be
loaded and used. Sequences created on version A.04.0x of the ChemStation
cannot be used in versions prior to A.04.01.
The following changes have been implemented in the sequencing user
interface and internal structure of the ChemStation, version A.04.01:
• The sequence table has been enhanced with an Append Line button.
• The hard-coded link between sample information and specific vials has
been removed to allow for easier cut/copy/paste operations on sequence
lines.
• The partial sequence screen has been equipped with a print button.

156 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.03.0x of the ChemStation

• Better integration of Sequence Summary into the sequence user interface.

Sequence Summary Setup is now accessible through a new menu called
Sequence Output.
• Sequence recalibration table has been eliminated from the method.
• A new sample type has been implemented to allow for quality control
samples. Control samples can be used to verify the system’s suitability to
run a defined set of analyses prior to running the real samples. If the
defined system suitability criteria are not met, the sequence can be
programmed to stop before running the real samples.

Upgrading from A.03.0x methods

The modifications introduced in version A.04.01 of the ChemStation are
transparent to the user but may have implications for customized systems. The
additions and enhancements necessitated changes to the internal data
structures of the sequence engine and the ChemStation method.
Methods created on previous revisions of the ChemStation can still be loaded
and used. Methods created on version A.04.0x of the ChemStation cannot be
used in versions prior to A.04.01.
• The Sequence Recalibration Table has been removed from the method.
• The data structure of the ChemStation method now has additional fields to
accommodate method change history information. When saving
modifications to a method, you have the choice to add a modification
comment along with the operator name and date to document why the
change was made.
• The calibration user interface has been modified. The graphical and logical
representation of the calibration table has been rearranged. The logic of the
internal data structures has not been modified except for the added
support of control samples in the sequence.
• The internal storage format of the quantification data has been changed
from single precision to double precision floating point format (see
“Internal Storage Format of Quantification Data” on page 156).

Installing ChemStation for LC Systems 157

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.03.0x of the ChemStation

Transferring User Report Styles

Customized Report Styles you have added using the Add to Report Styles
menu item of the report layout view are normally transferred automatically
during the update.

This only works if you installed the ChemStation software into the same directory as the
NOTE previous version and did not remove the HPCHEM directory prior to your update.

Manually transferring Report Styles

To manually transfer user defined report styles copy your original Repstyle.reg
from the old CORE directory (default HPCHEM\CORE) to your current
software CORE directory and transfer the report templates (*.frp) from the
old REPSTYLE subdirectory.

158 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.04.0x of the ChemStation

Upgrading from A.04.0x of the ChemStation

A.05.01 of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the HISTORY directory on the ChemStation CD-ROM
to determine if you need to revalidate your system.
The following paragraph outlines the enhancements in revision A.05.01 of the
ChemStation that have an impact on backward compatibility.

A.05.0x Default Methods Use the Enhanced Integrator

The default integration algorithm used by revision A.05.01 and later of the
ChemStation is the Enhanced Integrator. In previous revisions, the default
integration algorithm used was the Standard Integrator. Data analysis
methods could be converted to the Enhanced Integrator. See “Enhanced
Integrator” on page 155 and the “Understanding Your ChemStation”
handbook.
If your environment requires your quantitative results to be backward
compatible with results obtained with previous versions of the ChemStation
(A.04.02 and earlier), you need to build your data analysis methods using
DEFOLDxx.M instead of DEF_xx.M (where xx indicates CE, GC, LC or MS).

Installing ChemStation for LC Systems 159

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.04.0x of the ChemStation

A.05.0x Methods May Use Enhanced Peak Purity

The user interface of the Peak Purity function available in the ChemStations
for CE, LC and LC/MS has been simplified, but required modifications to the
peak purity parameters stored in the data analysis method. If you convert to
the Enhanced Peak Purity function, the previous spectra settings are saved in
a text file called SPCOPS.OLD in the method directory. Once you have
converted a method to use the Enhanced Peak Purity function, you cannot
revert back to the original method. Make a backup copy of your original
method before converting.
If your environment requires your quantitative results to be backward
compatible with the peak purity results obtained with previous versions of the
ChemStation (A.04.02 and earlier), you can opt not to use the Enhanced Peak
Purity function and continue using the standard one.

160 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.05.0x of the ChemStation

Upgrading from A.05.0x of the ChemStation

A.06.01 of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the HISTORY directory on the ChemStation software
family CD-ROM to determine if you need to revalidate your system.
The following paragraph outlines the enhancements in revision A.06.01 of the
ChemStation that have an impact on backward compatibility and may require
you to change your documentation and Standard operation procedures (SOP),
because they include additional selections in the user interface.

A.06.0x Verification (OQ/PV) methods changed

The methods used for operational qualification and performance verification
of the Agilent 1100 system were enhanced. The standard methods can be used
by selecting the Standard Tests from the Options menu in the Verification view
of the ChemStation. Additional software verification tests were also added
that are used by the ChemStation A.06 OQ/PV service (contact Agilent
Technologies for details).

Changes in the enhanced integrator

The advanced baseline option was added to the enhanced integrator
introduced in revision A.04.01. The tangent skim option was enhanced with
respect to A.05.01. The default setting for both options did not change with
respect to A.05.01.

Calibration point weights

Calibration points weights 1/Y and 1/Y2 were added to the calibration table.

Report to HTML
The report now also allows to generate HTM files for direct posting on a web
server.

Installing ChemStation for LC Systems 161

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.06.0x of the ChemStation

Upgrading from A.06.0x of the ChemStation

A.07.01 of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the HISTORY directory on the ChemStation software
family CD-ROM to determine if you need to revalidate your system.
The following paragraph outlines the enhancement in revision A.07.01 of the
ChemStation that has an impact on backward compatibility and may require
you to change your documentation and Standard operation procedures (SOP),
because they include additional selections in the user interface.

Improved Sequence Table Function

The sequence filldown utility allows the user to change sequence table column
settings for specified vial ranges. Sequence table columns can be selected and
values entered for method, sample type, updating response factors and time.
Sample name and file name can be given a prefix and autoincremental
number.

162 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.07.0x of the ChemStation

Upgrading from A.07.0x of the ChemStation

A.08.01 of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the HISTORY directory on the ChemStation software
family CD-ROM to determine if you need to revalidate your system.
The following paragraphs outline the enhancements in revision A.08.01 of the
ChemStation that have an impact on backward compatibility and may require
you to change your documentation and Standard operation procedures (SOP),
because they include additional selections in the user interface.

Support of new Agilent 1100 Series Hardware

The ability to control the new Capillary LC system that is designed for
increased sensitivity and for analyzing limited sample volumes was added.
The software now supports the Agilent 1100 Series well-plate autosampler,
which is available in a standard and thermostatted version.

New ChemStation Plus Security Pack

With revision A.08.0x, you can upgrade the Chemstation product to the
Chemstation Plus security pack supporting the FDA requirements of CFR 21
Part 11.

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9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.08.0x of the ChemStation

Upgrading from A.08.0x of the ChemStation

A.09.0x of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the HISTORY directory on the ChemStation software
family CD-ROM to determine if you need to revalidate your system.
The following paragraphs outline the enhancements in revision A.09.0x of the
ChemStation that have an impact on backward compatibility and may require
you to change your documentation and Standard operation procedures (SOP),
because they include additional selections in the user interface.

Support of new Agilent 1100 Series Hardware

The ability to control the new Agilent 1100 Series Fraction Collector and the
Preparative Scale 1100 Autosampler.

Support for the Agilent 1100 Series Purification System

The A.09.0x ChemStation software supports the recently introduced Agilent
1100 Series Purification System for HPLC and LC/MS.

Support for Microsoft Windows 2000 Professional

With revision A.09.0x, the software is supported on Microsoft Windows 2000
Professional as well as on Windows NT Workstation 4.0.

Support for Version J.02.00 GPIB Drivers

The A.09.0x ChemStation software is supported with the newer J.02.00 GPIB
drivers and SICL libraries for the 82350A and 82341C GPIB cards.

No Support for 35900D A/D Card

Due to issues with the 35900D A/D card, which is based on the older ISA bus
standard, the A.90.x revision of the ChemStation will no longer support this
hardware.

164 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Upgrading from A.09.0x of the ChemStation

Upgrading from A.09.0x of the ChemStation

A.10.01 of the ChemStation is a major revision, introducing new functionality

and a number of defect fixes. Revalidation of the analytical computer system
may be required. Refer to the HISTORY directory on the ChemStation software
family CD-ROM to determine if you need to revalidate your system.
The following paragraphs outline the enhancements in revision A.10.01 of the
ChemStation that have an impact on backward compatibility and may require
you to change your documentation and Standard operation procedures (SOP),
because they include additional selections in the user interface.

Support of new Agilent 1100 Series Hardware

The ability to control the new Agilent 1100 Series Fraction Collectors:

G1364B Preparative Scale

G1364C Analytical Scale
G1364D Micro Fraction Collector

The Agilent Chemstation supports the new 40 funnel tray for high volume
collection for high flow rates and the new tray for Eppendorf tubes 80.5 ml,
1.5 ml and 2.0 ml).
The A.10.0x ChemStation software supports the recently introduced Agilent
1100 Series Purification System for HPLC and LC/MS. version A02.01.

Support for Microsoft Windows XP Professional

With revision A.10.0x, the software is supported on Windows XP Professional
(service pack 1a) as well as on Microsoft Windows 2000 Professional (Service
pack 2 and Service pack 3).

Support for Version L.02.01 GPIB Drivers

The A.10.0x ChemStation software is supported with the newer L.02.01 GPIB
drivers and SICL libraries for the 82350A/B and 82341C GPIB cards.

Installing ChemStation for LC Systems 165

9 Upgrading From a Previous Revision of ChemStation
Upgrading from A.09.0x of the ChemStation

XML Interface
The new XML based interface has been implemented to link the Chemstation
to LIMS and knowledge management systems.

166 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Importing HPLC ChemStation (Pascal Series) Files

Importing HPLC ChemStation (Pascal Series) Files

Overview
The file system used on the HPLC ChemStation (Pascal Series) is significantly
different from the file system available in the MS-DOS environment. The disk
drive format used by the HP 9000 Series 310/332/362 Pascal ChemStation
systems is called LIF (Logical Interchange Format).
The PC and LIF file systems are not directly compatible. To transfer data
between the two, it is necessary to use special conversion utilities.
The LIF utility to convert files between PC and LIF is located in the directory
support\lif on the ChemStation software family CD-ROM. To use it, you need
3.5-inch flexible disk drives in your Pascal ChemStation computer and PC.

The PC must be operating with MS-DOS or Windows 95 since both utilities are
NOTE incompatible with the Windows NT operating system, neither Windows 2000 or XP.

Installing ChemStation for LC Systems 167

9 Upgrading From a Previous Revision of ChemStation
Importing Pascal ChemStation files using the LIF Utility

Importing Pascal ChemStation files using the LIF Utility

Before You Start

Please note that the HP Copy and LIF Utility programs are not compatible with the Windows
NOTE NT operating system, Windows 2000 and Windows XP. Any file conversions from the
ChemStation (Pascal Series) must be performed on PCs running MS-DOS or Windows 95.

Before getting started with the conversion, do the following:

1 Format a disk on the Pascal system.
2 Install the LIF Utility on your PC by creating a directory called LIF, copy the
lifutil.exe file from the disk to this directory. It is most convenient to set up
the LIF program as an icon in your ChemStation group, even though it is a
non-Windows application.To do this, you need to create a so-called PIF-file
for the LIF Utility. PIF stands for program information file. Start the
PIF-Editor from the Windows Program Manager Main group and fill in the
screen as shown in Figure 23.

168 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Importing Pascal ChemStation files using the LIF Utility

Figure 23 Setting Up the Program Information File for the LIF Utility

3 After saving the Program Information File for the LIF Utility, add it as an
icon in your ChemStation group to make it the active group, go to the
Program Manager screen in Windows, select File, New, Program item. Fill in
the screen as shown in Figure 24.

Figure 24 Setting up the Program Manager icon for the LIF Utility

The icon can be changed to the icon shown above by selecting the Change Icon button on
NOTE the right and selecting from the menu of icons.

Installing ChemStation for LC Systems 169

9 Upgrading From a Previous Revision of ChemStation
Importing Pascal ChemStation files using the LIF Utility

Converting ChemStation Data Files using the LIF Utility

1 Copy the Pascal ChemStation data file to the flexible disk using the Pascal
ChemStation File Manager utility. For example, use the Copy File(s)
softkey. You are prompted for the source and target file names.
2 Place the Pascal disk with the data file(s) in the PC’s flexible disk drive.
3 Start the LIF Utility on your PC.
4 Specify the source drive (LIF drive name) as A: and supply the Pascal
ChemStation data file name (LIF file name).
5 Supply the directory for the target file on your PC.
6 Switch the File Name Conversion mode to Extension using the F3 or F4
function keys.
7 In case duplicate file names occur, choose Make unique name in the
Duplicate File name settings.
8 Set the file name conversion to Binary Image: no format change.
See the example screen as shown in Figure 25.

Figure 25 Setting up the LIF Utility for a Pascal Data File Conversion

170 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Importing Pascal ChemStation files using the LIF Utility

Converting Libraries from the HPLC ChemStation (Pascal Series) to

the ChemStation using the LIF Utility
Copying CONVLIB.G to Pascal
1 Insert the Pascal formatted disk into your PC’s flexible drive.
2 Start the LIF program by double clicking on the icon created above.
3 From the main menu choose DOS to LIF <ENTER>. Fill in the LIF screen as
shown in Figure 26. With the cursor on the File format conversion field, use
the F1/F2 keys to switch conversions choices. After completing the screen,
select F5 to start the conversion.

Figure 26 Setup for Copying CONVLIB.G from DOS to PAWS Disk Using the LIF Utility

Verify that your Pascal system is version 4.1x/5.1x or higher. To determine the version on
NOTE your system, go to the TOP level and type VER <RETURN>. The current macro for the Pascal
part of the conversion only runs on the specified versions.

Installing ChemStation for LC Systems 171

9 Upgrading From a Previous Revision of ChemStation
Importing Pascal ChemStation files using the LIF Utility

Converting the Pascal library to .PLF format

1 Take the disk with CONVLIBG to the Pascal system and go to the File
Manager screen. First, rename the file from CONVLIBG to CONVLIB.G, then
copy CONVLIB.G to the DATA: volume using the copy command.
2 Go to Data Editor and type the following commands:
3 MACRO CONVLIB.G <RETURN>
4 CONVLIB <RETURN>
5 Select the library for conversion from the list. A .PLF file is created using
the first 5 characters of the library name. For example, DEMODAD.L
becomes DEMOD.PLF.
6 The library conversion process must go through each spectra in the library
and the time involved depends on the size of the library. Each entry
conversion requires about 20 – 30 seconds. If you have more than one
library to convert, do not leave the Pascal ChemStation Data Editor screen.
Start the macro again by typing CONVLIB <RETURN>
7 When the conversion to .PLF is complete, return to Top Level.
8 Translate the .PLF files to ASCII format and copy the file to disk as follows:
• Insert the flexible disk in the drive
• Type EX *FILER. <RETURN>
(Hint: Do not forget the period after Filer!)
• Type T
• Translate what file? YOUR.PLF <RETURN>
• Translate to what? #3:YOUR.ASC

Converting the .PLF files to ChemStation Library (.UVL) files

1 Place the Pascal disk with the .ASC file(s) in the PC’s flexible drive.
2 Go to the LIF program and select LIF to DOS from the Main Menu. Fill in
the screen as shown in Figure 27 on page 173. When the cursor is on the
LIF file name field, pressing the F6 key twice lists the contents of the
flexible disk. The F4 key can then be used to select the appropriate file. For
the file format conversion, again use the F1/F2 keys to switch to the choice
shown. In the Copy as field, leave the file name as the system has selected.
The file will be renamed later.

172 Installing ChemStation for LC Systems

 Upgrading From a Previous Revision of ChemStation 9
Importing Pascal ChemStation files using the LIF Utility

Figure 27 Setup for Copying .PLF file from Pascal to DOS Using LIF

3 In the Windows File Manager, select Rename from the File menu to change
the name of the file to YOUR.ASC.
4 Go to the ChemStation Spectra Library View and from the File menu select
Import Pascal Library.
5 Select the .PLF file from the file selection dialog box, then click the OK
button. You are prompted to edit the library header information. If
desired, click OK. Each spectra must again be processed so the time for
this step depends on the size of your library. It takes about 8 seconds per
entry. When the conversion is complete, you are prompted to enter a file
name which has a .uvl extension. The default path for spectral libraries is
C:\HPCHEM\SPECLIBS.
This completes the conversion process for spectral libraries using the LIF
Utility. The .uvl file can now be used independently or incorporated as part of
a method for automatic library searches.

Pascal Method and Sequence files cannot be imported into the ChemStation.
NOTE

Installing ChemStation for LC Systems 173

9 Upgrading From a Previous Revision of ChemStation
Importing Pascal ChemStation files using the LIF Utility

174 Installing ChemStation for LC Systems

Agilent ChemStation for LC Systems
Installing Your ChemStation

10
Supplementary Information
Agilent ChemStation Revision Code Convention 176
Agilent Technologies Response Center Support Services 177
Calling the Agilent Technologies Analytical Response Center 178

Agilent Technologies 175

10 Supplementary Information
Agilent ChemStation Revision Code Convention

Agilent ChemStation Revision Code Convention

Revision numbers are created according to the scheme outlined below:

P.RR.xx
P- Denotes the identifier of the series of a product. This letter is identical to
suffix character in the product number.
Example:
The first A in the product number G2170AA defines that revision code starts
with a series identifier A. It is current Agilent Technologies policy not to
change the series identifier unless the software changes in purpose or
function. The second A in the product number stands for the American
English version.
RR- Represents the major revision number. Changes in this code typically includes
significant software enhancements that may require complete revalidation of
the system for customers operating in regulated environments. These
revisions may also include defect fixes and documentation changes.
xx- Represents the minor revision number. These revisions correct software
defects and are generally isolated in nature. A minor revision change usually
does not require revalidation of the system for customers operating in
regulated environments. These revisions only include defect fixes that do not
affect the accuracy of the handbooks.

176 Installing ChemStation for LC Systems

 Supplementary Information 10
Agilent Technologies Response Center Support Services

Agilent Technologies Response Center Support Services

Agilent Technologies offers different options to help you obtain the

professional assistance you need to achieve maximum productivity with your
Agilent Technologies analytical software. This service is available worldwide
from centers based in Australia, Europe, and the USA.
The Agilent Technologies network of Analytical Response Centers (ARCs)
provides access to support professionals who help you resolve operational
difficulties, and offer assistance and advice on running Agilent Technologies
analytical software. Traditionally, this support is given over the telephone but
may also be extended to remote support via modem when you authorize us to
do so.
Your Agilent Technologies analytical software includes complimentary
Response Center support for a period of 90 days after installation of the
product. The duration of the support may be extended to one year or longer at
very competitive rates. In addition, you can sign up for a material update
service entitling you to automatically receive any software revision upgrades
when they are released along with regular delivery of the Software Status
Bulletins containing important information on known problems and available
work-around solutions for your Agilent Technologies analytical software.
For more information on how to register for these services, please contact
your local Analytical Support Representative.
Your local Analytical Support Representative will also provide information on
available consulting, customization, development and training services on
Agilent Technologies analytical software products.
Agilent Technologies Response-Center support and update services are
subject to your local country’s prices, terms and conditions ruling at the time
you place your order.

Installing ChemStation for LC Systems 177

10 Supplementary Information
Calling the Agilent Technologies Analytical Response Center

Calling the Agilent Technologies Analytical Response Center

When you call the Agilent Technologies Analytical Response Center, please be
at your computer and have the product documentation at hand.
We recommend that the following information be readily available:
• the registration packet label with the product number, revision code, and
license registration number of your analytical software
• the exact wording of any error messages that the system produced
• a list of the instrument modules including firmware revisions that are
connected to the PC. You may use the Serialnumber information from the
Agilent ChemStation (Method/Run control>Instrument (in Full Menu view
only) > Serialnumbers and Columns) to generate this list.
• a printout of the DrWatson.Log file (see “Who Is Dr. Watson?” on page 143)
• a full printout from Windows System Information
• a description of the scenario that leads to the failure.

178 Installing ChemStation for LC Systems

Index
A B COM board, 85
commands reference, 20
address backup
computer problems, 130
default GPIB, 81 directory, 146
computer settings, 23
GPIB, 68, 79 strategy, 139
config.reg (configuration register), 125
AddUserReportStyle, 150 bootp, 52
configuration
advanced power management, 45 bootp server, 109
Agilent 1100 Series LC, 103
Agilent 1100 Series, 66, 67 buffer handling, 113
editor, 103
autosampler, 67 buffer overflow, 133
instrument devices, 106, 108
CAN cabling, 66 buffer overrun, 117 paths, 100
connecting, 66 bus address, 64 configuration editor, 99
controller area network (CAN), 103
configuration register, 125
diode-array detector (DAD), 67 C connecting
GPIB cabling, 66
cable printer cable, 44
pumping systems, 67
GPIB, 57, 72, 78 connector
variable wavelength detector
(VWD), 67 remote, 66, 78 remote, 82
Agilent 1100 Series LC cabling, 66, 78 controller area network
configuring, 103 CAN Agilent 1100 Series, 103
Agilent 1100 system access, 103 Agilent 1100 Series, 66, 103 Agilent1100 Series, 66
Agilent 35900 dual channel interface, 81 cannot create file, 130 converting Pascal files, 167
connecting, 81 cannot find file, 122 coresidence, 146
Agilent 82341, 53, 54, 58 CD-ROM CTL board, 85
Agilent 82341 GP-IB card recordable, 141 customized report templates
switch settings, 55 changing importing, 150
Agilent 82350, 53, 55, 58 area calculation mode, 154
Agilent ChemStore, 22 checkdisk utility, 130 D
APM, 45 checksum, 88
DAD
area calculation mode, 154 ChemStation
Agilent 1100, 67
Autoadd Button, 120 connecting Agilent 1100 Series, 66, 67 HP 1040, 81
autosampler connecting HP 1050 Series, 77 HP 1050, 77
Agilent 1100, 67 connecting HP 1090 LC, 72 DAP board, 85
HP 1050, 77 connecting miscellaneous
def_lc.m, 100, 123
autostart macro failed, 124 instruments, 81
def_lc.s, 100, 123
GPIB select code, 107
uninstalling, 38
CHKDSK, 130, 137
coexecution, 146

Installing ChemStation for LC Systems 179

Index

default F HP 1050 Series, 77

GPIB addresses, 68, 76, 79, 81 autosampler (ALS), 77
file initialization failed, 123
method, 100, 123 diode-array detector (DAD), 77
path settings, 101 FILES setting, 128 GPIB cabling, 78
sequence, 100, 123 filter photometric detector, 108 multiple wavelength detector
default GPIB addresses, 68 firmware revisions, 83 (MWD), 77
default sequence, 100 fluorescence detector, 81 pumping systems (PMP), 77
deleting HP 1046, 81 variable wavelength detector
user report style, 148 (VWD), 77
detector G HP 1090 liquid chromatograph, 72
Agilent 1100 diode-array, 67 connecting, 72
G1315A DAD, 133 GPIB cable, 72
Agilent 1100 variable wavelength, 67 GPF, 143
electrochemical, 81 HP 82335, 46, 53
GPIB hpced02.exe, 99
filter photometric, 108 Agilent 1100 addresses, 68, 71
HP 1040 diode-array, 81 cable, 57, 66, 72, 78
HP 1046 fluorescence, 81 connector, 72
I
HP 1047 refractive index, 81 HP 1050 addresses, 79 I/O config utility, 99
HP 1049 electrochemical, 81 HP 1090 addresses, 76 I/O port conflicts, 119
HP 1050 diode-array, 77 select code, 107 importing
HP 1050 multiple wavelength, 77 switch settings, 55 customized report templates, 150
HP 1050 variable wavelength, 77 GPIB addresses, 68, 79 Pascal files, 167
refractive index, 81
Agilent1100, 68, 71 initialization problems, 133
device configuration dialog, 103
default, 68, 76, 79, 81 installation qualification, 88
diagnosing general protection faults, 143 HP 1050, 79 installation verification, 88
diode-array detector, 81 HP 1090, 76 installing
Agilent 1100, 67 GPIB cables, 72 hardware, 42
HP 1040, 81 GPIB interface, 42 INSTQ, 89
HP 1050, 77
instrument log book
directory
backup, 146
H buffer overrun, 117
hardware instrument not found, 122
Dr. Watson, 143
installing, 42 instrument types in configuration
drwatsn32.exe, 144
editor, 102
drwatsn32.log, 144 requirements, 45
integrator
drwatson.exe, 143 hardware address, 109, 110
enhanced, 155
drwatson.log, 143 host name, 113
interface, 81
dual channel, 81 how-to information, 19
dual channel, 81
dual channel interface, 81 HP 1040 diode-array detector, 81
interrupt line, 64
connecting, 81
IOCFG, autoadd button, 120
E HP 1046 fluorescence detector, 81
iocfg.exe, 99
connecting, 81
electrochemical detector, 81 IQ, 88
HP 1047 refractive index detector, 81
HP 1049, 81 IRQ conflicts, 120
HP 1049 electrochemical detector, 81
enhanced connecting, 81
integrator, 155

180 Installing ChemStation for LC Systems

 Index

L path settings S
default, 101
LAN communication select code
PCS section, 123
troubleshooting, 116 ChemStation, 107
power fail, 116
license registration GPIB, 107
print spooler, 126
utility, 122 sequence
printing messages, 128
license registration number, 26 default, 100, 123
printing problem, 128
license, no valid, 121 upgrading, 156
printing problems, 126
logical unit, 64 server
product structure, 21
network, 141
pumping systems
M service pack 3, 46
Agilent 1100, 67
serviceinfo macro, 178
MAC address, 109, 110, 113 HP 1050, 77
show me button, 19
macro programming guide, 20 SICL interface name, 64
magneto-optical disk drives, 140 Q signal distribution module, 85
MCO/MEM board, 85 software
qualifying
method
operation, 94 requirements, 45
default, 100, 123 software certificate and registration
quantification data
upgrading, 157 packet, 26
internal storage format, 156
mobility report, 154 software start-up problems, 121
multi-instrument configurations specified file does not exist, 123
printing, 126
R
support, 165
multiple wavelength detector recordable CD-ROM, 141 switch settings
HP 1050, 77 reference files, 88 Agilent 82341 GPIB card, 55
MWD reference information, 19 system controller, 64
HP 1050, 77 refractive index detector, 81 system exception, 123
HP 1047, 81 system status
N remote not ready, 122
cable, 66, 78
network
connector, 82
server, 141
remote cable, 73, 78
T
network-server based installation, 30
report task-orientated information, 19
non-HP computers, 43
mobility, 154 TCP/IP, 50
not ready state, 122
report templates test
not ready system status, 122
importing, 150 verification, 94
NT Workstation, 46 translating Pascal files, 167
resource conflicts, 44
revalidation, 155, 159, 161, 162, 163, tutorial, 19
O 164, 165
online core, 121 revision history, 18 U
operation qualification, 94 revision numbers, 176
UNC, 33
uninstalling the ChemStation, 38
P universal naming convention, 33
path setting, 28

Installing ChemStation for LC Systems 181

Index

upgrading, 146
methods, 157
sequences, 156
user report style
deleting, 148
user.mac, 124

V
variable wavelength detector
Agilent 1100, 67
HP 1050, 77
verification
test, 94
VISA interface name, 64
VWD
Agilent 1100, 67
HP 1050, 77

W
win.ini, 37, 123
update, 107
Windows 2000 Professional, 46
Windows 95, 46
Windows 98, 46
write-once media, 140

X
XML interface, 166
XML support, 37

182 Installing ChemStation for LC Systems

www.agilent.com

In This Book
Use this handbook when you install
the Agilent ChemStation for the first
time or when you make changes to an
installed system. This handbook
describes how to install the initial
software, how to add further
instrument modules, how to
configure your analytical system, and
how to verify that the installation
and configuration are complete and
operational.
This handbook lists the PC hardware
(see “Agilent ChemStation PC
Hardware Details" on page 42) and
software (see “Operating System
Requirements" on page 46)
requirements that need to be met in
order to install and operate the
Agilent ChemStation successfully
and explains PC configuration details
that you will find useful when
upgrading your computer, optimizing
your system, or when trying to solve
an installation-related problem. You
may skip these chapters if your
Agilent ChemStation was fully
installed by Agilent Technologies.
 Agilent Technologies Deutschland GmbH 1994-
2003

Printed in Germany
07/03

*G2170-90007*
*G2170-90007*
G2170-90007

Agilent Technologies