Habitat:
1.     Fasciola : bile duct of sheep, cattle, goat and buffalo.
    2.     Schistosoma mansoni: radicals of inferior mesenteric vein draining large intestine and ileum
    3.     Heterophyes heterophyes : small intestine of man, cat, dog and fish eating birds
    4.     Diphyllobothrium latum: small intestine of man and fish eating mammals.
    5.     Taenia saginata : small intestine of man (proximal jejunum).
    6.     Taenia solium: small intestine of man.
    7.     Hymenolepis nana: small intestine of man mainly children.
    8.     Trichuris trichura: large intestine (caecum and rarely appendix).
    9.     Entrobius vermicularis: large intestine (caecum and appendix) mainly of children and female>male.
    10.    Capillaria philippinensis: small intestine in jejunum and birds act as reservoir host.
    11.    Ascaris : small intestine of man
    12.    Ancylostoma duodenal : small intestine of man , mainly in jejunum and proximal ileum but rarely in duodenum
           except in heavy infection.
    13.    Strongyloids stercolaris: free living in soil or parasite in small intestine mainly duodenum and upper jejunum.
    14.    Entamoeba histolytica: lumen of large intestine
    15.    Giardia limblia: duodenum, upper jejunum , bile ducts and gall bladder
    16.    Balantidium coli: caecum and colon of man, pig, guina pig and rat
    17.    Isospora belli: duodenum and proximal jejunum of man only
    18.    Cryptosporadia: beneath the brush border of gastric and small intestine mucosa of man, calves, kittens,
           puppies and rodents
Parasite that can cause dysentery:
    1.     Trichuris trichura
    2.     Schistosoma mansoni
    3.     Entamoeba histolytica
    4.     Balantidium coli
Parasites that can cause appendicitis:
    1. Taenia saginata and solium (by their active gravid segment cable of creeping independent of the parent)
    2. Trichuris trichura
    3. Entamoeba histolytica (amoebic appendicitis)
Egg:
All are mature except:
Ascaris, Ancylostoma, Fasciola, Trichuris , Diphyllobothrium latum and Capillaria.
Only Strongyloids that have partially embryonated egg.
   1. Fasciola: operculated and large.
   2. S.Mansoni: lateral spine and contain fully developed miracedium.
   3. Heterophyes heterophyes: very small, operculated has knob like thickening on opposite sides of the shell and
       contain fully developed miracedium.
   4. Diphyllobothrium latum: immature, operculated when it become mature the embryo is ciliated hexacanth
       embryo.
   5. Taenia: 2 egg shell, the inner is thick brown striated embyrophore, the outer is thin hyaline capsule
       surrounding hexacanth embryo or oncosphere.
   6. H.Nana: 2 egg shell inner one containing 2 knob at opposite sides from which 4-8 filaments run in space
       between outer and inner shell, it contain hexacanth embryo.
   7. Trichuris trichura: barrel shaped, polar plugs and immature.
   8. Entrobius vermicularis: larvated (mature), asymmetric flattened at one side and convex at the other
   9. Capillaria: thick shell egg, unembyronated, flattened bipolar plugs and striated outer shell.
   10. Ascaris: unfertilized egg: more elongated its present when female> male – fertilized egg: contain 3 shells inner
       most us non permeable vitelline, middle is thick transparent and outer is mammillated albuminous layer (bile
       stained) or may be absent (decorticated).
   11. Ancylostoma: immature, thin shell, 4 cell stage, in constipated or well ventilated faeces may be morula or
       larval stage.
   12. Strongyloids: thin shell partially embryonated.
Intermediate host:
   1.   Fasciola : Lymnea trunculata.
   2.   Schistosoma: Biomphalaria
   3.   Heterophyes: 1st is Pirenella conica – 2nd bolty and boury
   4.   Taenia saginata: beef
   5.   Taenia solium: pig and man in case of cysticercosis.
   6.   Diphyllobothrium : 1st copepod crustacean eg. Cyclops or diaptomus – 2nd fish eg. Salmon, perch or trout.
   7.   Hymenolepis nana: man
   8.   Capillaria: fresh water or brackish water fish.
Mode of infection:
   1. Fasciola: eating or drinking contaminated food (water vegetations eg. Lettuce) or drinks containing encysted
      metacercaira.
   2. S.Mansoni: penetration of unbroken skin by swimming in infective water or mucus membrane if he drinks
      water infective by cercaria.
   3. Heterophyes heterophyes: eating imperfectly cooked fish containing encysted metacercariae.
   4. Diphyllobothrium: eating imperfectly cooked fish containing Plerocercoid larva
   5. Taenia saginata: eating imperfectly cooked beef meat infected by Cysticercous bovis.
   6. Taenia solium: eating imperfectly cooked pork meat infected by Cysticercous cellulosae.
   7. Hymenolepis nana: autoinfection by ingesting the egg.
   8. Trichuris: contamination of hand or food by larvated egg.
    9. Entrobius vermicularis: external and internal autoinfection and inhalation
    10. Capillaria: autoinfection by larva or thin shelled egg, hyperinfection or ingesting imperfectly cooked fish
        containing larva.
    11. Ascaris: ingesting fully mature egg containing 2nd rhabditiform larva by contaminated food, hand or water or by
        house flies.
    12. Ancylostoma: penetration of skin in soil.
    13. Strongyloids: penetration of skin in soil.
    14. Entamoeba histolytica: ingestion of cyst form in contaminated food or drinks
    15. Giardia : ingestion of cyst form in contaminated food or water- autoinfection- man to man and mechanical by
        house flies
    16. Balantidium coli: : ingestion of cyst form in contaminated food or drinks
    17. Isospora belli: ingestion of mature sporulated oocyst form in contaminated food or drinks
    18. Cryptosporadia: ingestion of mature oocyst through: contaminated food and drinks- close contact with calves-
        autoinfection- direct person to person and inhalation
Autoinfection:
    1.   H.nana
    2.   Entrobius vermicularis
    3.   Capillaria
    4.   Giardia
    5.   cryptosporadia
Infective stage:
    1. Fasciola : encysted metacercaria
    2. Schistosoma : cercaria
    3. Heterophyes : encysted metacercaria
    4. Diphyllobothrium latum: Plerocercoid larva (wrinkled cuticle and non segmented)
    5. Taenia saginata: cysticercous bovis
    6. Taenia solium: cysticercous cellulosae
    7. H.nana: egg
    8. Trichuris: larvated egg
    9. Entrobius: larva or thin shell egg
    10. Ascaris: 2nd stage rhabditiform larva
    11. Ancylostoma: 3rd stage filiariform larva
    12. Strongyloids: 3rd stage filiariform larva
    13. Entamoeba histolytica: mature 4 nucleated cyst
    14. Giardia : cyst
    15. Balantidium coli: cyst
    16. Cryptosporadia: mature oocyst
    17. Isospora belli: mature sporulated oocyst
Diagnostic stage:
    1. Fasciola: egg
    2.    Schistosoma mansoni: egg
    3.    Heterophyes: egg
    4.    Diphyllobothrium latum: egg or sometimes gravid segments
    5.    Taenia: gravid segment
    6.    H.nana: egg
    7.    Trichuris: egg
    8.    Entrobius: egg
    9.    Capillaria: egg, adult or larva in stool or adult in duodenal aspiration
    10.   Ascaris: egg in stool, larva in bronchial and gastric washing
    11.   Ancylostoma : egg (4 cell stage)
    12.   Strongyloids: rhabditiform larva or rarely egg in purgation of diarrhea or larva in sputum especially if the adult
          in the lung
    13.   Entamoeba histolytica: in dysenteric stool  trophozoit, well formed stool cyst
    14.   Giardia : diarrhoeic stool  trophozoit, well formed stool cyst
    15.   Balantidium coli: trophozoit with or without cystic stage
    16.   Cryptosporadia: oocyst
    17.   Isospora belli: oocyst
Specimen:
    1.    Fasciola: stool and duodenal aspiration
    2.    Schistosoma: rectal swab- rectal biopsy- stool- sigmoidoscopcy and biopsy
    3.    Heterophyes, diphylloborthrium, taenia, H.nana, trichuris, Ancylostoma : stool
    4.    Entrobius: find egg under nails, perineal scraping or anal swab (early in the morning before washing)
    5.    Capillaria: stool or duodenal aspiration
    6.    Ascaris: bronchial or gastric wash or stool
    7.    Strongyloids : stool or sputum
    8.    Entamoeba histolytica: stool – sigmoidoscopcy , liver: X-ray and ultrasonography
    9.    Giardia : stool- duodenal aspiration and entero test
    10.   Balantidium coli: stool
    11.   Isospora belli: stool
    12.   Cryptosporadia: stool- entero test- intestinal biopsy and sputum examination
Concentration techniques:
    1.    Schistosoma: formol ether or kato technique
    2.    Ascaris: saline sedimentation, formol ether and MIFC
    3.    Ancylostoma , Strongyloids, giadria and isospora: zinc flotation technique
    4.    Cryptosporadia: formol ether and sheather's sugar flotation technique
Entero test:
    1. Fasciola
    2. Strongyloids
    3. Giardia limblia
    4. Cryptosporadia
Separate sexes:
    1. Schistosoma mansoni : male  6-9 testes , female  elongated ovary and the intestinal caeca unit
       prequatorial
    2. Trichuris: male  coiled posterior end, 1 long copulatory spicule , female straight caudal end
    3. Entrobius : male  die after fertilization, female long pointed tai, double set of genetalia, prequatorial
       valve, ,mouth opening posses 3 lips and esophagus is double bulbed
    4. Capillaria : male  single spicule and the tail contain 2 papillae, female  value in esophageal region and
       subterminal anus
    5. Ascaris: mouth 3 lip, cylindrical esophagus , male  coiled posterior end, female  straight posterior end.
    6. Ancylostoma: male  copulatory bursa and 2 spicules , female  straight posterior end.
    7. Strongyloids: male curved tail and 2 spicules, female vulva at posterior 1/3
Male organs:
    1.   Fasciola and D.latum  cirrus and cirrus sac
    2.   Trichuris, E.vermicularis and capillaria  single copulatory spicule
    3.   Ascaris and Strongyloids  2 minuit spicules without copulatory bursa
    4.   Ancylostoma 2 minuit spicules with copulatory bursa
Esophagus:
    1.   Trichuris : schistome
    2.   E.vermicularis: double bulbed
    3.   Ascaris: cylindrical and larva is rhabtiform
    4.   Ancylostoma: club shaped and 1st, 2nd and 3rd stage larvae are filiariform while 4th stage is rhabditiform
    5.   Strongyloids: filiariform
Soil transmitted disease:
Environment  warm, high humidity, heavy rainfall, moisture retaining soil and dense shade
    1.   Trichuris
    2.   Ascaris
    3.   Ancylostoma
    4.   Strongyoids
Causes of malabsorption (MA) and malnutrition (MN) :
   1.   Capillaria (MA)
   2.   Diphyllobothrium (MN contribute the host for B12)
   3.   Ascaris (MN contribute the host for vit A and C)
   4.   Ancylostoma (MA  anemia and hypoprotenemia)
   5.   Strongyloids ( MA  steatorrhea)
   6.   Giardia (MA  steatorrhea, B12 and D-xylose)
   7.   Isospora (MA)
Pneumonitis:
   1.   S.mansoni( bilharizial cor pulmonle)
   2.   Ascaris (loeffler's syndrome)
   3.   Strongyloids
   4.   Ancylostoma (pribronchial or pneumonitis)
Cutanous lesions:
   1.   Fasciola (ectopic fasciolasis)
   2.   S.mansoni (pruritis)
   3.   Ancylostoma (ground itch)
   4.   Strongyloids( erythema- intense itching and peticeal hemorrhage)
   5.   Entrobius (nocturnal itching in anal region)
   6.   E.histolytica( due to rupture of liver abscess)