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PHOENIX
lnitrument

USER MANUAL
cD-0412

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Before using centrifuge, please carefully read úis user manual for efficient operation and safety.
Contents

Safety Reminder I
1. Specifications J

2. Declaration of Conformity J

3. Required Operational Conditions.. 4

3.1 Basic operational conditions 4
3.2 Transport and storage conditions.... 4
4. Installation 4
4.1 Location 4
4.2 Connection of the power cord and grounding 5

5. Structure 5

6. Operation panel 6
7. Rotor Preparation 7
7.1 Prepare úe samples 7
7 .2InjecÍ the samples into tubes 7
7.3 Keep the tubes balanced
7.4 Inspect the 7
7.5 Symmetrically load centrifuge tubes into rotor 7
8. Operation.. I
8. 1 Normal operation...... I
8.2 RCF operation. l0
8.3 Pulse operation 10

10

9.1 Cleaning l0
10. Troubleshooting.. t2
10.1 Possible problems and solutions t2
t"
10.2 How to open the door....... .t. t2
I l. Instructions for the rotor and tubes............ l3
I l.l Rotor instructions l3
11.2 Tubes 15

t6
I 3. Ordering information.. l6

17

14,1 Warranty of centrifuge.. t7

14.2 WarrarÍy of the rotor ............ t7
After-sales service t7
 Safety Reminder
Common safety precautions
Carefully read the following safety precautions for a thorough understanding.
o Follow úe instructions and procedures described in this manual to operate this centrifuge safely.
O Carefully read all safety messages in this manual and the safety instructions on the centrifuge.

O Safety messages are labeled as indicated below. They are in combination with signal words of
*WARNING" and "CAIJTION' with the safety alert symbolAto call your attention to items or

operations that could be dangerous to you or other persons using this centrifuge. The definitions of
signal words are as follows:

/?\ yTappING : PersonatDanger

Warning notes indicate any condition or practice, which if not sfictly observed, could result in personal
injwy or possible death.

/r\ CAUTION : Possible damage to centrifuge

Caution notes indicate any condition or practice, which if not strictly observed or remedied, could result in
damage or destruction of the centrifuge.

NOTE : Notes indicate ar'area or subject of special merit, emphasizing either the product's capability or
common errors in operation or maintenance.

o Do not operate the centrifuge in any manner not desçribed in this User When in doubt or
have any troubles with this centrifuge, ASK FOR HELP.

o The precautions described in this User Manual are carefully developed in an attempt to cover all the

possible risks. However, it is also important úat you are alert for unexpected incidents. Be careful

operating this centrifuge.

ll\ wARNTNG
o This centrifuge is not explosion-proof. Never use explosive or flammable samples.
o Do not install the centrifuge in or near places where inflammable gases are generated or chemicals are
stored.

a Do not place dangerous materials within 30cm of the centrifuge.

o Prepare all necessary safety measures before using samples that are toxic, radioactive or contaminated

with pathogenic micro-organisms. Use of these is at your own responsibility.

a If the centrifuge, rotor and açcessories that have been contaminated by solutions with toxic,
radioactive or pathogenic materials, clean it according to the decontamination procedure as specified.

O If you requte service at site, please sterilize and decontaminate the centrifuge in advance, and then
notifu the service center the details of the materials and procedure,
a To avoid electrical shocks, insure hands are dry before handling the power cord or turning on/off the
power switch.

a For safety purposes, do not enter within 30cm around this cenÍrifuge when it is in operation.
a While the rotor is rotating, never release the door lock.
a Unauthorized repairs, disassembly, or modifying the centrifuge except by our service center are
strictly prohibited.

lA caurroN
o This centrifuge must be located on a firm and level table.
o Make sure the centrifuge is horizontal before running.
a Make sure the angle between the door and cover is greater than 70 degrees when opening the door.
a Be careful not put your fingers or hands between the door and cover while the door is open.
a Do not move or reloçate the centrifuge when it is running.
o If fluid spills in úe rotor chamber, please promptly clean aad dry with a dry cloth to avoid sample
contamination.

O Ensure to remove any objects and fragments of the tubes dropped inside úe rotor chamber before
running the centrifuge.

a Cautions with rotor

(1) Always check for corrosion and damage on the rotor surface before using it. Do not use the rotor

if an abnormality is found.
(2) Do not set the speed beyond the allowable minimum speed of the rotor kits (rotor and adapters).

Make sure to run it below the allowable maximum speed.

(3) Do not exceed the allowable imbalance.

(4) Use the rotor and tubes within their actual capacities.

(5) If the rotor is attached with a lid, ensure it is tightened before operation.
a If any abnormal condition occws during operation, please stop it immediately and contact our service

center. Noti$ the service center is a warning code if displayed.

a Vibrations are likely to damage the centrifuge, contact our service center if abnormality observed.

2
1. Specifications
Maximum speed 4500rpm (300-a500rpm), increment: 1 00rpm

MaximumRCF 2490x g, increment: 1009

Maximum capacity l0Íú)< 12, 15ml>< 8

Timer 30 seconds -99 minutes-HolD, continuous operation

Driving Motor Brushless DC motor

Safety devices Door interlock. over-speed detector. over-temperature

automatic
internal diagnosis

Single-phase, 1 10V-240V 50Hzl 60H2, 3 A

Dimensions (mm
) (L) 255x (w) 245>< (H) l4o

Weight 6kgs

Speed/RCF switch. Pulse operation. Processing display. Voice

â".D*dnref,mn,-çf, ,Çgmf,g#,ãh$xd ards :

EN 61010-1
EN 61010-2-10

Construction in accordance with the following EMC standards

EN 61326-1/ FCC Part 15 Subpart B/ IECS 001

Associated EU guidelines:
EMC-guidelines : 2004/ 1 0S/EC
Instrument guidelines: 2006 19 5 IEC

This ISM device complies with Canadian ICES-001.

Cet appareil ISM est conforme àla norme NMB-001 du Canada'

Changes or modifications not expressly approved by the parfy responsible for compliance could
void the

user's authority to operate the equipment.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against

harmful interference when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with

the instruction manual, may cause harmful irúerference to radio communications. Operation of this
equipment in a residential area is likely to cause harmful interference in which case the user will
be
required to correct the interference at his own expense.
J

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3. Required Operational Conditions

3.1 Basic operational conditions

(l) Power: 110V-240Y 50Hzl60Hz,3A.
(2) Ambient temperature: 2'C40"C.
(3) Relative humiditY: <80%.
(4) No vibration and airflow around.
(5) No electric dust, explosive and corrosive gases around.

3.2 Transport and storage conditions

)
(I Storage temperature: -40"C-55'C.
Q) Relative humid§: <93o/o.

4. Installation
This seçtion describes the instructions that you should abide when install the centrifuge to ensure your
safety and ttre optimum performance. Before moving the centrifuge, the rotor must be removed.

A wanNnIG
o Improper power supply may damage centrifuge.

o Make sure the power source conforms to the required power supply before connecting.

4.l Location
( 1 ) Place this centrifuge on a firm. flat and level surface, ensure the four feet of this centrifuge stand
on

the counter firmly.Avoid instalhng on a slippery surface or surface prone to vibration.

(» Ideal ambient temperature is 20'Cl5'C, avoid placing the centrifuge in direct sunlight if
temperature

exceeds 30"C.
( 3 ) Keep clear of the centrifuge at least 10cm on both sides and at least 30cm behind it to guarantee
the

cooling efiiciency.
(4) Keep away from heat or water to avoid sample temperature issues or centrifuge failures.
4

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4.2 Connection of the power cord and grounding
 wmNnrc
O To avoid electrical shocks, el§ure your hands are dry when touching the power cord'

o This centrifuge must be grounded properly.

An minimum l0A outlet providing a sufficient ground is required, and this must meet local safety

requirements.

5. Structure

Door lock hook

Ert"il

Door release hole

+ií,

Figure 5.1 Front view of the centrifuge

rc;l Air vents

hole
Power inlet

switch

Figure 5.2 Rear view of the centrifuge

5
6. Operation panel

@@@
@
e
t2 J 4 5

Figure 6- I Operation panel

Item Symbol Name Function

1 S€led Select button Press úe button to choose the program which you want to
modify.
2 Puls€ The speed can be accelerated and held at úe speed when
Pulse button
pressing Pulse on.

Press the button to open the door The button is not available
3 Doú Oper/ lock button
when the centrifuge is running.

Press the button to start running. centrifuge will brake to

4 Starí Stop button
stop running if pressed during
Clockwise rotate to increase progÍam values. Rotate
5 Parameter button anti-clockwise to decrease parameter values.

^\ Press the button, shift between speed and RCF display.

Speed area Lock status Time area

+ á @
45 12
-100rpm ^,n

Figure 6-2The main interface

6
Main interface is as figore 6-2. The speed is set to be 4500rpm, úe door lock is released and úe running
time is 12 minutes. When speed symbol*is rotating, this indicates the centrifuge is running. If the
rotation is faster, the speed is higher. Temperature of chamber is displayed and cannot be controlled. Time
symbol@dlsplays the ratio of working to time setting. The total time setting is divided into 10 sections.

7. Rotor Preparation

7.1 Prepare the samples

T.2lnjectthe samples into tubes

CAUTION
o Do not overload samples into the centrifuge which will cause leaking.

a Do not exceed the actual capacrty allowed in the user manual.

7.3 Keep the tubes balanced

O Although the centrifuge can accept sample balancing by eye, we recoÍlmend that you keep this
centrifuge in a well-balanced condition to extend its life expectancy.

O Never intentionally run the centrifuge under an unbalanced condition even though the allowable
imbalance is not exceeded.

7.4 Inspect the rotor

Check the rotor for corrosion or scratches before using.

A ceurtoN
a If any abnormality such as corrosion or scratches are found, stop using the ,rotor and contact our
service center.

a Only manufacturer's rotors must be used with the unit.

7.5 Symmetrically load centrifuge tubes into rotor

,/^
CAUTION
a Make sure the rotor lid is securely fixed on the rotor, as well as úe rotor and shaft are tightened.

Otherwise, the rotor may be moved offwhile rotating and cause damage to the centrifuge and rotor.
a Firmly tighten the rotor lid to the rotor.

7
 8. Operation

CAUTION
a Do aot push or lean against the centrifuge while it is running.
a Do not run the centrifuge when fragments or sample solutions are left in the centrifuge chamber.
Always keep úe centrifugal chamber clean.
a If the centrifuge makes strange noise during operation, stop it immediately and contact our service
center. Notifu them of the warning code if displayed.

8.1 Normal operation

Turn on úe power switch, centrifuge will display the running interface last time after passing the
selÊdiagnostic checks, see figure 8-l below:

:há @
xro0rcm
{§ 12 ^,n

Figure 8-l the last running interface

o Speed: 4500rpm. Rururing tímel. 12 minutes.

a The door loçk is released.

8.1.1 Set the operation programs

Press the src button to select required program. The parameter can be modifibd when the program is
I
flashing. Rotate the program button clockwise to increase parameter value. Rotate the program
button clockwise to decrease parameter value. Rotate Íhe program button faster, and the
parameter value will increase faster. The minimum speed increment is 100 rpm, úe minimum time
increment is I second.

( I ) Set the speed

a Press the select button src until the speed rpm is displayed.

a when the speed button is selected, the speed symbol will flash the speed value.
a rhe minimum speed value you can set 300rpm, the minimum increment is 100 rpm.

o Rotate program button clockwise ;=\r' to increase speed value. Rotate the program button
anti-clockwis" t,l' to decrease speed value.
a You çan speed-up set úe speed value by rotating program button C' quickly.

8
o There is a circulating function to increase/decrease the speed values. Rotate the program button

O a change settings from small --- large - maximum --+ minimum' Rotate the program
clockwise
button anti-clockwis" Õ ,o change settings from large -* small -- minimum
--- maximum.

O) Set the time

a Press select buttsn ffi, time value flashes in the time sefiing mode.

a Rotate the program button Ô to set running time from 30 seconds to 99 minutes.
o When the time displays HD, this is a continuous running mode.

8.1.2 Start the operation

/í:;\
( l) Press buttorÍr-J to slart running

a The door must be locked before rotor starts spinning.

a The timer will start once the rotor starts spinning, the screen displays the remaining run time.

O) View and modify the operation program§

o operation programs can be modified after the centrifuge reaches the set speed.

a Pressing the select button

** , returns the display to the program interface and displays setting

sírc to the desired program. When flashing, rotate parameter

prograil§. Press the select button

button Õ to modify values. Release the button after 5 seconds, and the centrifuge will return to
normal operation mode and run according to the new value.
o If the set time value has been modified, the operation time is not affected and will continue.
(3) Warning display
o If an error occurs during the operation, úe centrifuge will brake to stop automlicallV, and display the
error code on the time/display area. The error code can be checked in the table l0-1, and corrective

actions can be applied accordingly.

8.1.3 End the operation

( I ) The centrifuge will brake when it reaches the set time or@ button is pressed'

a When the rotor stops rotating, the centrifuge will start beeping to alert the operation has finished.

O) Open the door

a The door can be released automatically when the operation has stopped.

o With the door closed, you are able to press the ü button to open it.

a After ending the operation, the program will store the setting parameters of this operation, and will
recall these paÍameters when restarting the program.
(3) Open the door and take out the rotor and samples.
9
 8.2 RCF operation
(l) Turn on the power switch

Q) Set a RCF (Relative Centrifugal Force) value

A CAUTION
a Do not exceed the allowable maximum RCF value of the rotor and adapters.

a Press Íhe select button {3( s16 choose speed unit ,g, úe speed symbol will flash into RCF value
input status.
o If no button is pressed after the speed value has flashed after 5 seconds, úe input mode will be shut
down.

a Rotate program butto, Õ to input a RCF value, RCF increment islOxg.

(3 ) Set operating conditions

The other operation, please refer to the section 8. l.

8.3 Pulse operation

This function is used to remove the residual samples adhered to the interior of the tubes or for quick spins.

Note : The button works only while the rotor stopped and the door is locked.

(I ) Turn on the power switch and load the rotor to the shaft, tighten the rotor lid and make sure it is
in
secured position, and úen close the door.
Q) The centrifuge goes into preparation mode and displays last running valuesl.

(3) Presso* knob and hold, the centrifuge will speed up to the setting sleed. While releasing
the

o* knob dunng acceleratioq the centrifuge will start to decelerate and stop.

9. Maintenance

9.1 Cleaning
,/t\
CAUTION
a If do not follow the recommended instructions for cleaning or disinfecting this may damage the
centrifuge.
10
 ( 1) Centrituge
o If the centrifuge is exposed to ultraviolet rays for a long time, úe color of the door may be changed or
the label may be peel off. After using, cover the centrifuge with a piece of cloth to protect it from
direct exposure.
a If the centrifuge needs cleaning, clean it with a cloth or sponge moistened with a neutral detergent
solution.
o Sterilize the centrifuge by wiping with a çloth moistened withT}%o ethanol solution.

Q) Rotor chamber

A CAUTIoN
Do not directly pour water, neutral detergent or disinfectant solution into the rotor chamber, otherwise
'
fluids may leak into the drive units and cause corrosion or deterioration to the bearings.

o If the rotor needs cleaning, clean with cloth or sponge moistened with a neutral detergent solution.
Sterilize the centrifuge by wiping with a cloth moistened with/}% ethanol solution.
(3) Drive shaft
o We recommend regular maintenance for drive shaft. You can wipe the drive shaft with soft cloth, and

then apply a thin coat ofsilicon gÍease.

(4) Door
o Clean and sterilize the door using the same method as the section ( l ) above.
(5) Rotor
a To prevent cottosiono remove the rotor from rotor chamber. If not in use for a long term, then detach
the rotor lid and turn upside down to dry the tube holes and keep clean.
a
I

For sample leaks in the rotor, rinse the rotor wiú water. Apply a thin coat of silicon grease to the rotor

when it is completely dry. I

o The rotor should be checked every 3 months to ensure the tube and rotor holes keep are clean aÍrd

apply a thin coat ofsilicon grease.

1l
10. Troubleshooting

10.1 Possible problems and solutions

This centrifuge has a selÊdiagnostic function. If a problem occurs, an error/warning code will be displayed

on the time display screen, and úe operator can determine the malfunction with the alarm code below.
Symptom Causes Solutions

Nothing appears on the screen when -Building power circuit breaker - Remove the trouble and turn

the POWER is tumed on. trips. on the POWER.

Abnormal vibration - Rotor do not match with - Install again the rotor

spindle - Weighting scales, install

- Samples are imbalance symmetrically

E-02 - The door opened in running - Close the door immediately.

ID Door fault {lose the door, and then start

3l#
o
- The oruo' is pressed
to operate.
o.Ê while the door opening.
â'o
§: - The setting - Modify the speed value.
E-06 speed exceed the
ao)
9ã
õo. Set wrong speed
o^ allowable range.

o
E-10 86 - Read the service manual - Contact wiú service center
(D
-

Table 10-1 Possible problems and solutions

o Alarm codes E-1

- E-9 are related to incorrect operation/programming. You c{n continue running
the

centrifuge after implementing corrective procedures.

10.2 How to open the door

10.2.1 In the case of power on

/r\
CAUTION
o The door just can be opened while the power is on and rotor stops rotating.

( l) Turn on the power switch, release úe door automatically.

(2) The door will be released automatically once the operation is finished.

(3) It is available to release the door by pr"ffi button once the rotor stops.
t2

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t
10.2.21n the case of power outage

manually
The door cannot be opened automatically if úere is a power outage. It is available to be opened

as follows.

( 1) Ensure if the rotor has stopped rotating.

a Listen carefully to ensure no rotating sound can be heard'

(Z> Insert a screw driver into a hole to open door.

o Holes are located on the left and right sides of the unit.

O Insert a screw driver into the two holes and push forward to release the door.

1 1. Instructions for the rotor and tubes

A caurroN
o Read the instructions thoroughly, to properly load and use rotor.

O Do not exceed the allowable maximum speed of rotor. tube and adapters etc. Ensure the
allowable

maximum speed of adapten is lower than the rotor's maximum speed.

1 1.1 Rotor instructions

11.1.1 Rotor structure

adapter nut rubber cap

1í.-]

body mat Spindle hole

Figure l1-l The rotor structure
l3
11.1.2 Available rotors and adapters

Tubes Dimension Maximum

Rotor Maximum Radius
Tubes Per («DxL Adapters RCF
type speed (rpm) cm
Rotor mm) (e)
15nú con 6 17xl20 4s00 11 2490

1.5-5ml vacu t2 13'€.2 4500 9.8 2218

l3xl06 l1 2490

4-7 mTvacu t2 4500

t6*75 9.8 22t8

8.5-10 ml vacu 12 l6xl07 4500 11 2490

2,7-3
(EU) Írú t2 11Á6 4500 9.8 2218
collection tube

7.5-8.2
(EU) nú t2 t5§2 4500 11 2490
collection tube

Table 1 1.1 Rotors and adapters

11.1.3 Notice

o The centrifuge rotor can separate samples with a dens§ lower than 2.}glml. If the samples density is

over 2.0g/m1, please calculate allowable speed depending on the following formula.
Allow Speed (rpm): Maximum speed x(2. 0(g/m1)/Sample dens rty (el *l))' D

11.1.4 Autoclaüng
Al2-10P rotor is made of plastic, cannot be high-pressure sterilization and UV irradiation, only ordinary
sterilization can be used.

14
11.2 Tubes

11.2.1 Cleaning and sterilizing tubes

O : Applicable X : Inapplicable
Conditions PA PC PP

Acidic (pH5 or lower) x x x

Acidiç (higher thanpH5 ) o o o
o Alkaline (higherthanpHg ) o x o
o
Cleaning fluids Alkaline (pH9 or lower) o o o
)
0e
Neutral (pH7) o o o
Warm water(up to 70"C) o o o
Ultrasonic cleaning Neutral detergent (pH7) o o o
115'C $.7kglcrfi) 3Ominutes o o o
Autoclaving lzl"C (1.0k{cr*)20 minutes x o o
a
o
t 126"C (t. ke/ cro2) 15 minutes X x x
N Boiling 15 to 30 minutes o o o
o
Ultraviolet sterilization 200-300nm x x x
Gas sterilization Ethylene oxide o x o
Formaldehyde o o o
PA: Polyallomer PC: Polycarbonate PP: Polypropylene
Table 11.2 Cleaning and sterilizing conditions for tubes
11.2.2 Cteaning PC tubes
PC material is low in chemical resistance against alkaline solutions. Avoid using neutral detergents with

pH higher than 9. Note that pH of some neutral detergents are still higher than 9 eyen if diluted according

to the manufacturer's instructions. Use detergent with its pH between 7 and9. I

11.2.3 Autoclaúng PA. PC and PP tubes

PAbegins softening at about 120"C, PC and PP at about 130"C. Autoclave PA tubes at
115'C $.7kgtc#)
for 30 minutes, PC and PP tubes at 121'C (O. tkg/cm2 for 20 minutes. If a certain temperature is exceeded,

the tubes may be defonned.

When use a sterilizing chamber, please operate as follows:

(l) Place tubes in vertical position, mouths upward. If tubes are placed sideways, they may deform into an

oval shape due to gravity.

(2) Remove locking nut and lid to prevent from deformation or rupture.

(3) Wait until the sterilizing chamber cools down to the room temperature before removing tubes.
15
11.2.4 Conditions and life expectancy of tubes

The life expectancy of plastic tubes depends on úe characteristics of samples, speed of the rotor used,

temperature applied and so on. When the plastic tubes are used for ordinary aqueous samples (pH between

5.0 and 9.0), their life expectancies are defined as follows.

Be operated at the maximum speed:

High qual§ tubes (PA. PC. PP): 30-50 operations

Ordinary tubes(PA. PC. PP): around 10 operations (Using in low speed can extend the tube
life)
Life expectancy of tubes also depends on the pretreatment conditions such as cleaning and sterilization,
lifetime can be cut down.
Notice: Do not use damaged or cracked tubes.

12. Calculate RCF

An RCF can be determined wiú the following calculation formula.
RCF:1.118>rryr2xl0-s

r-rotating radius, unit: cm; n-rotating speed, rnit: rpm

13. Ordering information

Cat. No. Model Descriptions
Clinical Clinical Centrifuge, with Al2-l0P rotor, Euro plug, 100V-240V,
cD-0412
Centrifuge 50Hzl60Hz
Accessories
cD04-18-5 Plastic rotor, 4500rpm, 15ml*8, used with qD0412

cD04-Al5 Rotor adapter, used with CD04-18-5, i2pcs$É

CD04-Al5plu ú
5 Al0P15 Rotor adapter mat, used with CD04-415, l2pcs/pk

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14. Warranty

14.1 Warranty of centrifuge

This centrifuge is guaranteed for two years from the date of delivery provided that it has
been operated and

maintained properly.

l4.2Warranty of the rotor

The rotor is guaranteed for 5 years from the date ofdelivery upon manufacturer. Please
pay attentioq do

not use the rotor once it has been corrosion or fatigue damage. The warranties of the
centrifuge and the

rotor become invalid in the case of the following conditions even if within the guarantee
period expires:

(1) Failures caused by incorrect installation.

(2) Failures caused by rough or improper handling.

(3) Failures caused by conveyance or relocation after installation.

(4) Failures caused by unauthorized disassembly or modification.

(5) Failures caused byusiag non-standard spare paÍts or accessories and

unauthorized modifi cation of

úe rotor or centrifuge.
(6) Failures caused by natural disasters including fire, earthquakes and so on.

(7) Consumables and parts have a limited guarantee period.

After-sales service
In order to ensure to operate cerúrifuge safely and efficiently, it is necessary for regular
maintenance. If

centrifuge has problems, do not attempt to repair it by yourself. Contact our sales or
service center.

PHOENIX INSTRUMENT GMBH

Heinkelstrasse 4
30827 Garbsen/Germany
Phone: +4915 13 l-908 1830
Fax: +4915131-9081839
Mail: info@phoenix- instrument. de
www.phoenix-ins trument. de
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