Compound Microscope - it is the difference in color
and brightness that makes
the object or an image
Microscope distinguishable.
- is an instrument used to see
organisms or objects that are
too small to be seen by the
naked eye. Parts of Microscope
Microscopy Illuminating Parts
- is the science of investigating - regulate the amount of light
very tiny organisms and that comes from the source.
small objects using the
microscope. Magnifying Parts
- make the object look larger
History or bigger.
- play the most important role.
14th Century - European Artisans
16th Century - Simple Microscope Mechanical Parts
17th Century - Renaissance - function as support and for
Period adjusting the different parts
1625 - first introduced the term of the microscope.
microscope by Francesco Stelluti &
Federico Cesi
1665 - Robert Hooke
Illuminating Parts
(Micrographilia)
Anton Van Leeuwenhoek - Illumination
father of microscope - the light source for a
microscope.
Compound Microscope - older microscopes used
- is called as such because it mirrors to reflect light from
consist of two kinds of lenses, an external source up
the ocular lens or eyepiece through the bottom of the
and the objective lens. stage.
- however, most microscope
Magnification now use a low-lens voltage
- ratio of an object's magnified bulb.
size to its actual size.
Iris Diaphragm
Resolution - a thin structure with
- it is the clarity of an image adjustable opening which
based on how close its lines opens and closes a screen of
to each other and still be metal plates.
resolved visibly. - controls the amount of light
that reaches the specimen.
Contrast
Condenser
- found beneath the stage. the specimen. it has a
- used to concentrate or focus magnification of 10x.
the light coming from the High Power Objective - the
diaphragm to the object longer tube with smaller
found on the stage or the opening and higher
specimen. magnifying power for
studying the detailed parts of
the specimen. it has a
magnification of either 43x or
45x or sometimes 40x.
Oil Immersion Objective -
Magnifying Parts same length as the HPO but
affording a much higher
magnification being stamped
Ocular Lens or Eye piece
97x or 100x. needs a special
- contains lenses.
oil to be placed on object
- detachable located on top of
studied
the craw tube which allows
the viewers to look through
and see the magnified image Mechanical Parts
of the specimen It has a
magnification of 10x. On/Off Switch
- it may contains a short hair - this switch on the base of the
which can be used as a microscope turns the
pointer. illuminator off and on.
Diopter Adjustment Base
- useful as a means to change - the U-shape or horse-shoe
focus on one eyepiece so as shaped iron portion which the
to correct for any difference microscope firmly rest.
in vision between your two - provides support and serves
eyes. as a stand.
Objectives Arm
- small tubes containing lenses - the C-shaped curved handle
screwed into the revolving of the microscope that
nosepiece which magnify the connects the base and the
object being examined or the body tube.
specimen. - serves to support the optical
- there are types of objectives. mechanism.
Low Power Objective - the - the part that you hold when
shorter tube with large lens you carry the microscope.
opening and with lower
magnifying power for Stage
observing the general outline - the platform attached to the
and locating various parts of arm which holds the slide
containing the specimen to Stage Height Adjustment
be viewed. (Stage Control)
- these knobs move the stage
Body Tube (Head) left and right or up and down.
- the body tube connects the
eyepiece to the objective Aperture
lenses. - the hole in the middle of the
stage that allows light from
Revolving Nosepiece the illuminator to reach the
- located at lower end of the specimen.
body tube serving for
attachinent of objective lens
and to facilitate the shift from
one objective to another.
Adjustment Knobs
- consisting of two pairs of
knobs which used for
lowering or raising the body
tube.
Coarse Adjustment Knobs
- the larger knob used for
faster movement when
focusing the LPO or locating Care of the Microscope
the outline of the specimen.
1. examine your microscope
Fine Adjustment Knob before using it for any
- the smaller knob for slow or defects, like misplaced or
little movement when missing parts. if there is
focusing the HPO for finer anything wrong with it call
detail of the object. the attention of the
- also used to set s sharper instructor, otherwise you will
focus of the object under the be held responsible for any
LPO. defects.
2. always carry the microscope
Spring/Stage Clips supported by the other hand.
- found on the stage used for never carry the microscope
securing the slide in position. hanging like a basket with
only one hand.
Specimen/Slide 3. place the microscope on the
- the specimen is the object table some six to eight
being examined. centimetres away from the
- most specimens are mounted edge.
on slides, flat rectangles of 4. do not separate loose parts,
thin glass. like the ocular or objectives,
as they may collect dust.
5. do not touch the lenses with
your fingers. Use lens paper
or soft dust cloth to clean
them.
6. check the following before
returning the microscope
after use:
a. the microscope is clean
and dry.
b. it is in upright position.
c. the mirror is in upright
position.
d. the LPO is in line with the
eyepiece or ocular.
