NUTRIENT AGAR
• SIMPLE BASAL MEDIA .
• MADE UP OF NUTRIENT BROTH
{PEPTONE WATER + MEAT
EXTRACT(1%) } & 2% AGAR.
• USES:
1.FOR GROWING PATHOGENIC
ORAGNISMS
2.FOR PREPARATION OF BLOOD AGAR
3.TO DETERMINE ANTIBIOTIC
SENSITIVITY TESTING.
•STERILIZATION:
BY AUTOCLAVING
BLOOD AGAR
• ENRICHED MEDIUM
• COMPOSITION : NUTRIENT AGAR & 5-
10% SHEEP BLOOD
• USES:
1.FOR GROWING MOST OF THE
PATHOGENIC ORGANISMS.
2.TO DIFFERENTIATE HEMOLYTIC
COLONIES FROM NON-HEMOLYTIC
COLONIES.
3.IN THE PREPARATION OF POTASSIUM
TELLURITE BLOOD AGAR.
• STERILIZATION:
BY AUTOCLAVING
CHOCOLATE AGAR
• ENRICHED MEDIUM
• CHOCOLATE IN COLOUR
• IS PREPARED BY HEATING THE BLOOD
AGAR AT 55°C FOR 2 HOURS.
• USES:
1. FOR GROWING FASTIDIOUS
ORGANISMS SUCH AS HEMOPHILUS,
NEISSERIA , PNEUMOCOCCUS.
• STERILIZATION
BY AUTOCLAVING.
TCBS AGAR
• THIOSULPHATE BILE SALT SUCROSE
AGAR
• SELECTIVE & DIFFERENTIAL MEDIA
• USES:
1.INHIBITORY TO GRAM POSITIVE
ORGANISMS,MOST COLIFORMS
&PROTEUS.
2.DIFFERENTIATES SUCROSE
FERMENTING (V.CHOLERAE) FROM
NON SUCROSE
FERMENTINGCOLONIES
(V.PARAHEMOLYTICUS)
• STERILIZATION:
BY AUTOCLAVING
XLD
• XYLOSE LYSINE DEOXYCHOLATE AGAR
MEDIUM
• SELECTIVE & DIFFERENTIAL MEDIUM
• DIFFERENTIATES XYLOSE FERMENTING
COLONIES(SALMONELLA& SHIGELLA)
FROM NON XYLOSE FERMENTING
COLONIES
• DETECTS H2S PRODUCTION(COLONIES
WITH BLACK CENTRE)
• USES: GROWTH OF SALMONELLA &
SHIGELLA SPECIES.
• STERILIZATION
DONE IN WATERBATHAT 50°C
DO NOT AUTOCLAVE
MULLER HINTON AGAR
• COMPOSITION:BEEF
INFUSION,CASEIN
HYDROLYSATE,STARCH,AGAR.
• USES:FOR ANTIBIOTIC
SUSCEPTIBILTY TESTING BY DISC
DIFFUSION METHOD.
• STERILIZATION
BY AUTOCLAVING
DCA
• DEOXYCHOLATE CITRATE AGAR
• SELECTIVE MEDIA
• USED FOR ISOLATON OF
SALMONELLA& SHIGELLA IN
STOOL.
LOWENSTEIN JENSEN MEDIUM
• SELECTIVE MEDIA FOR MYCOBACTERIUM
SPECIES
• SOLID MEDIUM WITHOUT AGAR
• EGG IS USED IN ITS PREPARATION AS
SOLIDIFYING AGENT
• CONSISTS OF BEATEN EGGS, MINERALSALTS ,
ASPARGINE, GLYCEROL & MALACHITE GREEN .
• GREEN COLOUR OF THE MEDIUM IS DUE TO
MALACHITE GREEN WHICH IS A SELECTIVE
AGENT .
• M.TUBERCULOSIS TAKES 3-6 WEEKS TO
GROW.
• USES:
1.FOR GROWING MYCOBACTERIUM
TUBERCULOSIS & ATYPICAL MYCOBACTERIA.
2.FOR ANTIBIOTIC SUSCEPTIBILTY TESTING OF
M.TUBERCULOSIS.
• STERILIZATION
BY INSPISSATION.
MAC CONKEY MEDIUM
• DIFFERENTIAL MEDIA OR INDICATOR
MEDIA.
• COMPOSITION:
PEPTONE,LACTOSE,AGAR,NEUTRAL
RED , SODIUM TAUROCHOLATE&
WATER.
• CONTAINS NEUTRAL RED AS AN
INDICATOR
• USES:
1.FOR GROWING INTESTINAL
ORGANISMS.
2.TO DIFFERENTIATE LACTOSE
FERMENTER (LF) FROM NON-LACTOSE
FERMENTER(NLF) COLONIES.
• STERILIZATION
BY AUTOCLAVING.
RCM BROTH
• ROBERTSON’S COOKED MEAT
MEDIUM(RCM)
• CONTAINS COOKED MEAT.
• ALSO KNOWN AS COOKED MEAT
BROTH
• USES
1. FOR GROWING ANAEROBIC
ORGANIMS
2. FOR PRESERVATION OF STOCK
CULTURES OF AEROBIC
ORGANISMS.
SDA
• SABOURAD’S DEXTROSE AGAR
• NONSELECTIVE ISOLATION MEDIA
• MEDIUM HAS ACIDIC pH
• FUNGI CAN TAKE WEEKS TO GROW
BUT YEAST GROW IN 24-48 HOURS
IN THIS MEDIUM.
• USES:
1. FOR THE GROWTH &
MAINTANENCE OF PATHOGENIC
& NONPATHOGENIC FUNGI
FROM CLINICAL AS WELL AS
NONCLINICAL SPECIMENS.
2. FOR RECOVERY & TOTAL
COUNTING OF YEAST &MOULDS
IN ENVIRONMENTAL
MONITORING.
PEPTONE WATER
• LIQUID MEDIA
• CONTAINS PEPTONE, Nacl & WATER.
• USES:
1.FOR GROWING MOST PATHOGENIC
ORGANISMS.
2. FOR MAKING HANGING DROP
PREPARATION.
3. FOR TESTING INDOLE PRODUCTION.
4.FOR PREPARATION OF SUGAR MEDIA
5.GROWING BACTERIA FOR ANTIBIOTIC
SUSCEPTIBILITY TESTING.
• STERILISATION
BY AUTOCLAVING.
