Understanding

B12 Deficiency
RnB Protocol
V4, June 2023

from the B12

For testimonials
>>>
group, click here

This document has been produced by the “Understanding B12 Deficiency”

Facebook group, with the kind permission of Dr Greg Russell-Jones who has
generously provided all the research and evidence in the links attached.

Copyright B12 Oils

Contents
1. Why do you need the RnB protocol? 6. Riboflavin and Vitamin B12 (RnB)
2. What will the RnB protocol achieve? Treatment Protocol, important note and
3. Time Protocol for children.
4. Testing 6.1 Iodine
4.1 Mosaic Diagnostics Organic Acids Test 6.2 Selenium
(Oat) – Urine Analysis 6.3 Molybdenum
4.2 Thyroid Panel - TSH/T4/T3- Blood Test 6.4 Vitamin B2 (Riboflavin)
4.3 Doctors Data - Hair Toxic and 6.5 Vitamin B12 (Adenosyl/Methyl B12)
Essential Elements Analysis (HTMA) 6.6 Potassium
4.4 Standard Complete Blood Count 7. Problem Solving
(CBC) Panel & Other Blood Markers 7.1 Why Is the Protocol Not Working? Notes
5. Reactions From GRJ
5.1 Adrenaline 7.2 I Am Very Sensitive to Supplements
5.2 Over-Production of Adrenalin Side- Annex A – RnB Treatment Protocol Quick
Effects. Guide
5.3 Signs and Symptoms of Potassium
Deficiency (Hypokalemia) For videos by GRJ, click here
5.4 Depression and Anxiety >>>

The following conditions are associated with impaired methylation and vitamin B2/B12 deficiency.
Anyone who suffers from the following conditions may benefit from using the RNB protocol

Mast Cell Activation Syndrome Multiple food intolerance GERD

Autism Histamine Intolerance Irritable Bowel Syndrome
Sensory Processing Disorder Multiple Chemical Sensitivity Inflammatory Bowel Disease
Chronic Fatigue Syndrome FPIES and FPIAP Insomnia
Depression/Anxiety SIBO Sciatica and Neuropathic Pain
Hypothyroidism Dementia/Alzheimer's Disease Osteoporosis
Alopecia Diabetes/Pre-Diabetes Iron Deficiency Anemia

Disclaimer: Please note that any advice given is derived from scientific literature and
is of a general nature. For specific advice please consult your physician or medical
representative.

Please be aware that the information in this document is copyright, and as such
should not be reproduced in full or in part without the permission of B12 Oils.
 UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

1. Why do you need

the RnB Protocol?
All about methylation
This protocol has been developed by Greg Russell-Jones PhD to help
your body with a very important process called Methylation.
Very simply, you can liken each of the cells in our body to little
factories, and inside the factories there are over a thousand
different types of machines. Each machine performs a very
specialised chemical reaction, and every single one of those B2
machines is very important to make the things that our cells need Iron
to function properly.
B12
These machines are known as Enzymes, and they exist in the cells D
of all forms of living organisms. They enable the cells in our bodies
to produce the chemical reactions that are necessary for us to be
alive, including making energy.

Just like any factory, the cell needs to have the right “goods in” as
ingredients to actually make the enzymes (machines) functional in
the first place, and then secondly the enzymes need the right
ingredients to make their special product.

As you would expect, if a factory is missing a necessary

component or two, the end result will be a defective product. The
same is true of our cells. If we are deficient in a vital mineral or
vitamin our bodies will suffer.

Dr Greg Russell-Jones (GRJ) specialises in the intricacies of the

Methylation Cycle which uses over 200 different enzymes to work. The
main ingredient in this process is Vitamin B12, however, for our bodies
to use Vitamin B12 we need to have active Vitamin B2 (Riboflavin).

Copyright B12 Oils

 UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

Vitamin B2 (Riboflavin) has to be activated by enzymes

using three specific components: minerals Iodine, Selenium
and Molybdenum. Supplementation with vitamin B2 alone
will not work, unless there is also sufficient Iodine, Selenium
and Molybdenum (cofactors).
...for our bodies to
use Vitamin B12 we
Iodine Sel Moly B2 B12 need to have
needed to activate needed to activate
active Vitamin B2.

The active forms of B2 (FMN & FAD) are used by two enzymes
(MTHFR & MTRR) in the critical role of maintaining functional
Vitamin B12. Persons who are deficient in FMN & FAD rapidly
become deficient in active vitamin B12, which will then impact
all the enzymes that use Vitamin B6 as well. That will then Track your dietary intake:
have a knock on effect on our bodies being able to use Cronometer App
Vitamin B9 (Folate). Deficiencies in any and all of these We recommend using the
important vitamins will have a profound impact on our Cronometer" app to check if
health. your diet is providing the
nutrition you need (although
Unfortunately, the soil in many parts of the world is now it is lacking data on iodine
depleted of these essential minerals and changes in our and molybdenum).
eating habits has made getting sufficient minerals in our diet
very difficult.

So, how do we fix it?

Everyone requires the recommended daily intake (RDI) of
Iodine, Selenium and Molybdenum every day of their life,
hence supplementation must be continued or the diet must You cannot fix functional B12
be changed to ensure adequate intake of these essential deficiency unless you fix
minerals every day. functional B2 deficiency.

The RnB protocol has been developed to overcome

functional B2 deficiency in conditions such as Chronic
Fatigue Syndrome, Autism Spectrum Disorder, and recently
the protocol has been used for the treatment of Long COVID.
It is a preparatory step before injection or transdermal
application of vitamin B12. You cannot get enough vitamin
B12 to restock the brain of a
The protocol is the property of B12 Oils Pty Ltd. child or an adult from any oral
supplement, whether it be
lozenge, high dose oral or even
nasal delivery.
UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

2. What will the RnB

Gregory Russell-Jones PhD
is a specialist Biochemist
whose passion is utilising

Protocol Achieve?
his extensive experience
and scientific knowledge to
promote health in our
This protocol will enable Vitamin B12 to community.
work properly.

Having active Vitamin B12 is essential for all of us to prevent

irreversible nervous system degeneration and prevent
anaemia. B12 is the main ingredient for the biochemical
process called methylation, which is necessary for many
aspects of our mental and physical health, including
preventing memory loss, making energy, regulating sleep, ...people think that they can
removing histamines, gut health and much more. magically cure a condition
that has been present for
Vitamin B12 deficiency during pregnancy increases the years or even decades in a
risk for preterm labour, low birth weight and increased couple of days or weeks. You
infant mortality. have to rebuild or build
The brains of children with autism have been found to myelin, you have to fix 100
have greatly reduced levels of vitamin B12.
FAD dependent enzymes,
Please look at these research links for more info:
about the same number of
https://b12oils.com/research.htm
B6 dependent enzymes, and
around 200 methylating
enzymes. Once you have

3. Time
done this you have to rebuild
or build all the myelin in your
body, an amount of wiring
Depending upon how deficient you are in any of equivalent to that of a large
the ingredients, you may experience big reactions town or small city. It is very
when you start to supplement the missing naive to think you can or
minerals and vitamins. It is very important that even should rush this.
you do not rush into taking the RDI of any of the Further, many have other
minerals, but gradually increase them at a rate deficiencies. You have to fix
that is comfortable for you. Please become very
them all.
familiar with section 5. REACTIONS.

- Dr. Gregory Russell-Jones

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4. Testing
The RnB protocol is dependent upon
identification of deficiencies using these
tests: Dr Greg Russell-Jones uses
his own range to assess your
Doctors Data Hair Toxic and Essential Elements analysis data, which is often very
(HTMA) different to the “normal” test
Mosaic Diagnostics Organic Acids Test (OAT) – urine range stated on your result
analysis. sheet.
Thyroid TSH/T4/T3 – blood tests.

Can't get tested?

If possible, we do recommend getting TSH/T3/T4 as a minimum before starting
the protocol. If you are unable to get T3/T4 via your doctor, you may be able to
get this privately through a naturopath, as a home test kit, or order via
MyMedLab or BLT Labs. Many people have found it very helpful to do these
Thyroid tests every 3 months to monitor their progress and make any needed
adjustments to dosing the cofactors.

Many folks can't access testing due to financial constraints or other reasons.
This will mean that you're less able to personalize the protocol for your
deficiencies, however you may send your symptoms and any old labs to GRJ
and begin without adequate test results.

4.1 OAT test

For more information on OAT
Functional B12 deficiency is most easily established with the testing, visit:
OAT (see 4.1). Dr. Greg (GRJ) prefers Mosaic Diagnostics
(formerly Great Plains Lab), but if you can't get it then use b12oils.com/oat.htm
Genova Nutreval. You may require a doctor or naturopath to
order. You may also have success ordering directly via this link.

See the Facebook Guide in the “Understanding B12 Deficiency”

group on OAT test sources for more information on where you
can order the OAT test in your country.

For more information on

Pros: diagnosing B12 deficiency,
It provides a lot of biochemical information, especially visit:
useful for B2, B12 and Iron functioning.
Cons: vitaminb12deficiency.info/
It is very expensive. diagnosis.htm
It is just a snapshot of what you metabolized the day
before.
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OAT target lab values

MMA (methylmalonic acid): Normal 0.40 – 0.70

MMA is one of the main markers of Adenosyl B12 deficiency shown in the OAT. As AdenosylB12
deficiency increases, so too does the level of MMA. Hence a vitamin B12 replete person MMA
should be as low as possible.

Succinic Acid: Normal under 2.5

The first step in activating B2 is converting B2 to FMN. Lack of Iodine and/or Selenium is shown by
the increase in succinic acid and increase in the Quinolinic Acid: Kynurenic Acid ratio (see
https://b12oils.com/oat.htm)

Glutaric Acid: Normal 0.20 (range 0.05 – 0.20)

Increased glutaric acid indicates functional B2 deficiency and a reduced ability to burn fat for
energy. The second step in activating B2 uses Riboflavin kinase (a molybdopterin enzyme) to
convert FMN to FAD. If glutaric acid and succinate levels are normal, yet other B2 deficiency
markers are elevated, Molybdenum deficiency is likely.

Citrate: Normal under 150

Higher values can represent iron deficiency and inefficiency of energy metabolism, but
Hb/Hct/ferritin blood values will need to support this. Other examples of energy loss are elevations
in oxalate, glycolate, and lactate. The higher the values for these markers the greater the energy
loss.

Test prep
There are some foods that should be avoided 48 hours before the test (see information packet
that comes with the test), but in general GRJ would like you to continue with your supplements
(especially the RnB cofactors) so he can see if your particular ones are working.

Results and analysis

Members who have an OAT test and analysis from GRJ, please PM Vanessa Smith or Becki Warner
to join the " B2/B12 OAT Markers" group. In the OAT group we use a spreadsheet with Greg’s correct
ranges for OAT data, and additional information on what these levels mean. This makes it a lot
easier to track your progress on the protocol and we can all learn from GRJ’s analysis.

Note re: "range of normal": The OAT lab ranges are based on very sick people and so are not a
useful indication of B12 deficiency.

For people sending GRJ the OAT, it is very important that you have a good idea of your diet and
what supplements and doses you were on at the time of the test. The analysis that GRJ gives is of
the metabolism on the day you did the OAT test. He doesn't know if you are supplementing or not.
Most people send GRJ data from an old OAT, so as long as you haven't dramatically changed
what you were eating or what you were supplementing with, your metabolism generally is the
same now as then.
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4.2 Thyroid Panel - TSH/T4/T3 Blood Test

Thyroid function blood tests are standard tests that are easily available and show possible
deficiencies in Iodine and/or Selenium. In many countries they are available as inexpensive home
finger prick tests if your doctor is not willing to order them.

Thyroid panel target lab values

TSH: 0.5 - 1.5 mU/L (definitely under 2.0)

TSH above 1.5 indicates you need more Iodine, TSH is below 0.5 may mean you need less Iodine,
however this may drastically change when you start supplementing selenium & molybdenum.

T4: Should be in the top half of the range

If your T4 is in the bottom half of the range, it could indicate you need more Iodine. Iron is also
important for this step (see 7.1).

T3: Should be in the top half of the range

If your T3 is in the bottom half of the range, it could indicate that you need more selenium.

As you fix things you get higher demand and so TSH can go up temporarily and demand for
Molybdenum will go up as you make more FMN and that needs converting to FAD (active B2).

4.3 Doctors Data - Hair Toxic And Essential Elements Analysis

(HTMA)
See the Understanding B12 Deficiency group guide on HTMA test sources for more information on
ordering a test in your country.
Pros Cons
This helps identify Iodine, Selenium Your hair takes time to grow, so your results will be
and Molybdenum deficiency as several months behind.
well as other important minerals. You do have to be a bit careful with interpretation
of HMTA, GRJ prefers having the biochemical
backup of blood or OAT tests as well.

HTMA target lab values:

Iodine 0.50 – 1.8 ug/g Molybdenum 0.07 – 0.13 ug/g

Required for synthesis of T4 Essential for the conversion of FMN to FAD (The two
active forms of vitamin B2)
Selenium. Over 1.0 ug/g
Essential for conversion of T4 to T3
(https://b12oils.com/b2.htm)
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4.4 Standard Complete Blood Count (CBC) Panel & Other

The CBC is a standard test that is easily available. It helps GRJ identify functional iron deficiency.
Iron deficiency has been associated with lower vitamin D levels. Iron also has a major role in the
conversion of energy generated from the metabolism of fats, sugar and protein.
https://b12oils.com/iron.htm

Note: Pregnant women will not be able to use the iron targets since Haemoglobin and
Haematocrit drop during pregnancy, also cycling women may have trouble reaching this goal.

Standard blood test target lab values

Keep in mind that some of this is complex and is affected by many other factors. It’s difficult to “do
this yourself” but these are provided as guidelines. It’s always best to work with GRJ on
interpretation of labs.

Ferritin: Over 100 ng/mL (ug/L same)

As levels drop below 100 changes can be seen in the bone marrow, suggesting biochemical
deficiency. The level of citrate in the OAT changes when ferritin drops below 60 ug/L,
demonstrating biochemical iron insufficiency.

Haemoglobin (Hb): Over 14.5 g/dl: (144 g/L)

Levels can be above this in those who live in a high altitude. Once Hb levels drop below 14.5 iron
insufficiency results. Given that Hb is responsible for carrying oxygen, insufficiency in O2 max will
occur as Hb drops.

Haematocrit (Hct): Greater than 45% (0.450 L/L)

Normally this track very closely with Hb.

Creatinine
Bottom of the range can indicate a lack of methylation and some have found supplementing
creatine Monohydrate may help with fatigue until you fix your deficiencies.

Serum B12 – Optimal around 350-450 pmol/L (474-610 pg/mL)

Serum B12 levels of less than 250 pmol/L (339 pg/mL) should be assessed as at risk of B12
deficiency. This should be mirrored by markers of iron deficiency (Hb/Hct/ferritin). If serum B12 is
high, and yet iron markers are low, then this is "suspicious" and further investigation is warranted.
Serum B12 over 450 pmol/L is indicative of paradoxical B12 deficiency, BUT, lower values can
also be indicative of this, hence secondary markers are required. The incidence of Paradoxical B12
is so frequent that the "normal range" measures by Pathology laboratories has greatly distorted
the range for serum B12, hence OAT markers such as MMA and other B12 deficiency markers, such
as HVA/VMA, QA, KA, and 5HIAA, and pyroglutamate (a product from deficiencies in the sulphation
pathway) must be used to establish normality. Learn more: https://b12oils.com/paradoxical.htm
UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

MMA- should be as low as possible, so if it is "in-

range" it is high.

Vitamin D 40-70 ng/ml (100 – 175 nmol/L)

All members are
Vitamin D and vitamin B12 work together in stimulating
encouraged to gather the
myelination of nerves in the brain.
testing Dr. Greg Russell-
Jones recommends and
email their results to

5. Reactions enquiries@B12oils.com for

free consultation.

The protocol revolves around a very

gradual introduction of Iodine (I), Selenium
(Se) and Molybdenum (Mo).

Introduction is slow, as supplementing any of

these can have strong side effects. No one should start any
ingredient in this protocol
without having POTASSIUM
5.1 Adrenaline reactions foods and supplements
available. See sections
Understanding reactions are a big part of recovery from B12 5.3 & 6.6 Potassium for more
deficiency. information.

When you supplement ANY ONE of the deficient cofactors

leading to B12 (Io, Se, Mo, B2, Ferritin, Folate, B12) then the
cascade of energy from thyroid to B12 is ignited and
suddenly adrenalin production resumes. This can cause
potassium deficiency which can become serious if not
addressed quickly.

How to manage this:

Be very consistent in dosing (at least 2x day, every day)

Start with TINY amounts, some can only handle the head
of a pin to lower the effect.
Add potassium foods and supps as needed when
adrenalin symptoms start (see 6.6 Potassium).
Keep hydrated. No alcohol, no caffeine.
Adrenalin symptoms can start up days, weeks or even
months after starting or increasing any of the cofactor
supplements.
Some people find monitoring their pulse through the day
helps judge how much potassium is needed. The faster
the pulse the more potassium is required.
UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

5.2 Over-Production of Adrenalin Side-

Effects
Increased fatigue, joint or muscle pain, skin rashes, Adrenaline in children
photosensitivity, irritability, paresthesia, dizziness, sleep
disturbances, asthenia, muscle cramps, night sweats, In less extreme cases, most
hypertension, hypotension, headaches (especially children "react" with excess
migraines) and swollen glands. energy and trouble sleeping.
This is well managed with
Also reported are heavy perspiration, metallic taste in mouth, adequate potassium.
chills, nausea, bloating, constipation or diarrhea, low grade
fever, heart palpitations, tachycardia, facial palsy, tinnitus,
mental confusion, uncoordinated movement, pruritus, bone
pain, flu-like syndrome, conjunctivitis and throat swelling.

5.3 Signs and Symptoms of Potassium

Deficiency (Hypokalemia)
Mild hypokalemia is characterized by constipation, fatigue,
muscle weakness, and malaise. Muscle Cramps and Moderate to severe
Spasms, Muscle cramps are sudden, uncontrolled hypokalemia can cause
contractions of the muscles. Digestive Problems. Heart polyuria (large volume of
Palpitations. Muscle Aches and Stiffness. Tingling and dilute urine);
Numbness. Breathing Difficulties. Mood Changes. Irritable, encephalopathy in patients
hyper, quick temper. See section 6.6 Potassium for more info. with kidney disease; glucose
intolerance; muscular
paralysis; poor respiration;
5.4 Depression and Anxiety and cardiac arrhythmias,
especially in individuals with
Depression and anxiety can slowly creep up, even a week or underlying heart disease.
two after an increase. Severe hypokalemia can be
life threatening because of
You have two "happiness" hormones: dopamine and its effects on muscle
serotonin which are abnormally high in B12 deficiency. The B12 contraction and, hence,
deficient person is just like a person on SSRIs, addicted to their cardiac function.
own-overproduction of serotonin and dopamine. When you
start to fix the B12 deficiency, your levels of dopamine and
serotonin drop rapidly. Your body eventually adapts, but it
can take a long time, so you have to be very methodical and
gradual with every increase in the cofactors. Potentially follow
the advice that doctors suggest for coming off
antidepressants or other addictive drugs by as little as 1% per
day. Learn more: https://b12oils.com/receptors.htm
UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

6. Riboflavin &
Vitamin B12 (RnB) See Annex A for the quick
guide charts.

Protocol
The protocol assumes that you are low on
all of Iodine, Selenium and Molybdenum,
which has been assessed by analysis of
The RDI ranges listed
HMTA, OAT and/or thyroid markers.
represent minimum and
https://b12oils.com/rnb.htm
maximum ranges of daily
intake for a person who is
If you are already sufficient in Iodine, or Selenium or
replete in Iodine, Selenium
Molybdenum, as judged by these markers, then you do not
and Molybdenum. Persons
need to supplement the mineral you are sufficient in.
who are deficient may
initially need more until they
Everyone, regardless, does require at least the
become replete.
Recommended Daily Allowance (RDA) or Recommended
Daily Intake (RDI) of Iodine, Selenium and Molybdenum. If
you do not get this then the activation of vitamin B2 will be
compromised.

For simplicity there is a topical ISeMo oil product

Dose per 8 drops:

This is a combination of Iodide 150 ug, Selenite 55 ug, ISeMO: Combination of

Molybdate 100 ug, suitable for children and sensitive adults. Iodide 150 ug, Selenite 55 ug,
Adults will eventually need to top up with additional single Molybdate 100 ug.
cofactors. Find the oils here: b12oils.com/products.htm
Find this and other oils here:
Keep a record of your progress with the protocol. b12oils.com/products.htm

Many members find it helpful to note down the symptoms

that you are suffering with before starting the protocol. Then
whenever you change anything - record the date, the brand
of supplement, the dose and any side effects. Also record
your progress and improvements. This will help you judge
how quickly you can safely increase your cofactors in the
future.
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Important note
For children and those very sensitive, a slower paced
progression is often more successful. If you move too fast,
the start-up reactions may not be tolerable, resulting in a
premature abandonment of the protocol or inadequate time
for repletion of nutrients. What do I order?

As such, it may be advisable to start with an amount the size Isemo: for children
of a head of a pin, and increasing slowly from there. For
others, increasing by 1/4 or 1/2 drop is possible. Iodine, Selenium &
Molybdenum singles: For
If you find you and/or your child are able to tolerate without adults or when needing to
issue, you can increase the size of future titrations. Start low "top up" on Isemo
and slow.
Adenosyl/methyl B12: When
The protocol in the following pages represents an "ideal" reaching the B12 step
progression but most members find they need to go much
slower. Remember: sometimes the slowest option is actually B2 (Riboflavin): Not currently
the quickest. available in oil form.
Purchase plain riboflavin
powder, drops or a low dose
Protocol for children B complex (eg Berocca) from
a retailer near you.

Start with the mixed isemo oil.

If your child is very sensitive to most things, it's advisable to

begin with an amount the size of a pin head. For others,
starting with 1/4 or 1/2 drop may be tolerated.

Increase accordingly until 8 drops are reached, or you've

reached the desired amount on your personalized plan. It is
Depending on deficiencies,
not a race, only our bodies can tell us how quickly to
some families require a
progress. When at full isemo, resume protocol at step 6.4.
"topping up" of one or two
minerals only. It may be
Applying oils to children beneficial to order the
singles alongside the mixed
Caregivers should be aware that when applying to children, isemo oil.
oils may be absorbed into the caregivers skin. To avoid this,
consider using the backside of a spoon for application onto
the child, a gloved hand, or another protective implement.
Alternatively, wash hands immediately and thoroughly after
applying.
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6.1 Iodine
The iodine should be as either Sodium or
Potassium Iodide. Lugol’s Iodine does not
work; it must be Iodide.
Topical application avoids
interaction of Iodide with
Our data suggests that a suitable target for Iodine can be food or other supplements.
assessed by monitoring TSH, where a target of between 0.5
and 1.5 mU/L should be aimed for.

If your TSH is above 1.5, then you need more Iodine, if it is

below 0.5 you may need less Iodine and you can move
straight to Part 2: Selenium. Note - please re-test TSH after
3 months as this will likely change when you start to
suppliment se & mo. RDA for Iodine is 150-300
ug/day (every day)

Weeks 1-2 and Ongoing

Start with 50 ug/day for one week. Week 2 increase to 100

ug/day. Week 3 and ongoing, 150 ug/day.

A suitable product is the topically applied Sodium Iodide oil

which can be titrated up from one drop (~ 75 ug), to 4 drops
(300 ug). https://b12oils.com/order.htm
Our group is not a substitute
Foods to limit for working with Dr. Greg
Russell-Jones. We are only
Goitrogens, such as soy, Cassava, Broccoli, Cauliflower, here to spread the word and
Cruciferous veggies, and Kale (https://www.iodine- to share notes to learn from
resource.com/goitrogens.html). Also foods that have his brilliant take on the
cyanide (Thiocyanate & cyanoglycosides) such as biochemistry behind
chickpeas, maize/corn, flax seeds, almonds and stone fruit. methylation and B12. Please
keep that in mind before
Symptoms of over-supplementing with Iodine starting the basic protocol
and also before asking the
Flu-like symptoms, drowsiness, brain fog following Iodine other sick people for health
supplementation, and a drop in TSH below 0.5. advice.

Natural sources of Iodine

Seafood, Dairy, Eggs, Grains, and fortified products such as

Iodized bread and Iodized salt.
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6.2 Selenium
The selenium should be as Selenite salt,
but should not be Selenomethionine or
Selenium chelates, or Selenium amino
Topical application avoids
acids complex, each of which has been
interaction of Selenite with
shown not to work food or other supplements.

Weeks 3-6 and Ongoing

On Week 3, after 2 weeks of Iodide supplementation,

continue with the Iodide and add 25 ug/day Selenium. Week
4, increase to 50 ug/day. Week 5, increase to 100 ug/day.
Week 6 and ongoing, 200 ug/day. Adjust doses for children.
RDA for Selenium is 200 ug
for adults, and 55 ug for
A suitable product is the topically applied Sodium Selenite oil
children.
which can be titrated up from one drop (~ 27.5 ug), to 8
drops (220 ug).https://b12oils.com/order.htm

Avoid taking oral Selenite with:

Diatomaceous earth or charcol, fulvic acid, large dose

vitamin C or those with citrate as a preservative, as they can
reduce absorption of Selenite. Avoid any source of Calcium,
as these bind up the Selenite, thus avoid giving the material
in milk. Similarly avoid giving the material in acids,
particularly vitamin C and citric acid, as these reduce the
Selenite and make it non-available.

Natural sources of Selenium

Eggs, Tuna, Shrimp, Beef, Turkey, and wheat from Selenium

sufficient soils. Organic foods or gluten-free foods are often
low in Selenium as too wheat from selenium depleted soils
such as the UK, much of Europe, New Zealand, much of
Australia and many States in the USA.
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6.3 Molybdenum
The Molybdenum should be sodium or
ammonium Molybdate, NOT a
Molybdenum chelate or Molybdenum
Topical application avoids
amino acids complex.
interaction of Molybdate
with food or other
Weeks 4 and Ongoing supplements.

At week 4, continue with the Iodine and Selenium. Start with

50 ug/day Molybdenum and gradually increase to at least
200 ug/day. Some adults have been found to need as much
as 300 ug/day. Half the doses for children under 10.

A suitable product is the topically applied Sodium Molybdate

oil which can be titrated up from one drop (~ 50 ug), to 6 RDA for Molybdenum is
drops (300 ug). https://b12oils.com/order.htm 100-300 ug/day

Avoid taking oral Molybdate with:

A source of Calcium, as these bind up the Molybdate, thus

avoid giving the material in milk. Similarly avoid giving the
material in acids, particularly vitamin C and citric acid, as
these reduce the Molybdate and make it non-available.

Natural sources of Molybdenum

Lentils, peas, beans, oats, barley, bread, pasta, but only if

they are grown in Molybdenum sufficient soils.
UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

6.4 Vitamin B2 (Riboflavin)

GRJ recommends riboflavin. If you are using "R5P" take it away from
divalent cations (eg calcium, magnesium, copper, iron & zinc). He
also likes Berocca or other low dose B complex supplement which has
all the “B” vitamins, which can be broken into smaller pieces to titrate
up and also to spread dosing throughout the day.

3 Days after reaching full Isemo

After reaching full dose of isemo, you can slowly start to introduce vitamin B2. Start with trace
amounts, increasing gradually to 5-10mg/day depending on age. You must keep going with the
Iodine, Selenium and Molybdenum.

Note: You may need more B2 once the cofactors start activating it. Signs of B2 deficiency are
acne, red, crusty or cracking skin around the lips and corners of the mouth, or red, scaly, itchy skin
around the nostrils, ears, eyelids and genitals.

6.5 Vitamin B12 (Adenosyl/Methyl B12)

Once the Iodine, Selenium, Molybdenum and
B2 have been introduced then you can start You cannot get enough
on the mixed Adenosyl/Methyl B12 oil mixture. vitamin B12 to restock the
brain from any oral or nasal
https://b12oils.com/order.htm
supplement.

After reaching full B2

Start with just a trace amount. Apply the oil into an area of clean, dry, warm skin and rub it in like a
massage oil. If the child/adult can tolerate a "drop", then on the following week increase to 2 drops
and gradually increase to a whole squirt/dose. A full dose should be a bit more than a quarter size
on the skin, so be careful to make sure you get enough.

Some very deficient people have big reactions to a tiny amount of B12, in which case start with
as little as a pin-head of B12, and build up a little more, to two pin-heads, then to a drop, then start
to increase up to a full dose - over a period of as long as it takes. Ongoing it is a bit variable, some
people do two doses per day, but potentially you could split the dose to 2 x 1/2 per day. You have
to release the adrenalin "pressure-cooker" very slowly.

Important: apply B12 in a different location to iodine as it may impact absorption.

UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

6.6 Potassium See section 5.3 Signs & Symptoms of Potassium Deficiency (Hypokalaemia)

Natural sources of high Potassium

Dried Apricots (brown not orange), Lentils, Squash, Prunes, Jacket Potatoes, Coconut water,
Orange Juice (all contain more potassium than bananas).

Supplemental Potassium

If you need to supplement potassium always have it with food and a lot of water, as well as
adequate salt.

Potassium supplements are safe for healthy people. People with diabetes, insulin resistance,
impaired kidney function or who are using ACE inhibitors, angiotensin receptor blockers, digitalis,
heparin, Bactrim, Pentamidine or aspirin and ibuprofen should check with their doctor before
supplementing potassium. Potassium Chloride is a good option, many people use Bulk
Supplements brand or "Now" potassium citrate powder. Be sure to include sodium and
magnesium to ensure adequate electrolyte balance and absorption of potassium. Sea salt is a
good option here.

Tracking intake
The Cronometer App is a very good way to check your daily potassium intake. The DV (Daily
Value) for potassium is 4,700 mg for adults and children age 4 years and older, with a minimum
Adequate Intake for Males being 3400mg, Females 2600mg. For more information visit
https://ods.od.nih.gov/factsheets/Potassium-HealthProfessional/

7. Problem Solving
7.1 Why is the Protocol not Working?
There is always a reason, the problem is trying to find it.

"The issue is nearly always the activation of B2, BUT there is another issue which is very common
in kids with autism, and that is low ferritin. When ferritin is low, so is thyroid function (see
https://b12oils.com/iron.htm) and so now they have problems with the enzyme thyroid
peroxidase and its ability to put Iodine onto thyroglobulin and so you have a reduced ability to
make T4." (see 4.2)

This is a sort of catch 22, because if you don’t have functional B2, then you have problems with
iron absorption and then you have troubles making thyroid peroxidase, and then you have
troubles making functional B2. Probably the quickest and cheapest test is still TSH. If they are not
having success with Iodine, then TSH won’t drop." – GRJ
UNDERSTANDING B12 DEFICIENCY COPYRIGHT B12 OILS

Possible issues

Are you using some oral formulation that either has the wrong material in it, or has something
that inactivates Iodine, Selenium and/or Molybdenum? (See 6.2)
Having calcium or magnesium in an oral formulation or vitamin C, or having an oral Selenite or
Molybdate preparation that also has citrate in it.
Using liquid Iodine/Iodide solutions such as Lugols is poor, plus unbuffered Iodine/Iodide
solutions, which eventually change to Iodine.
Are you eating goitrogens or cyanoglycosides? (See 6.1)
Not actually applying the oils to warm, clean, dry skin. You shut down your skin lipids at around
16 C, and that would mean that for many the local temperature for half the year in most of the
UK, US and Canada would be below this.
Having magnesium baths (Epsom Salts baths).
High levels of calcium, fluoride, chlorine or bromine in the water can reduce mineral
absorption.
Infections and vaccinations put massive demands on B2 levels, requiring more cofactors.
Very high consumption of Vitamin A can cause orange/brown patches on the skin and use up
a lot of your active B2, which reduces the active B2 available for methylation.

7.2 I am very sensitive to supplements

Go very, very slowly
Some people have needed to use micro scales to measure out tiny amounts of oral cofactors
and build the amount up very slowly, or use GRJ’s mixed I/Se/Mo oil which has a very low dose
in one drop (19/7/12 ug) and the B Group oils which can both be started just with a pin head
amount.
Make sure you have plenty of Potassium food or supplements available and monitor your
pulse to address signs of potassium deficiency immediately.
If you are very B12/B2 deficient you may find supplementing the important products made by
the methylation process will help until you are replete – such as Creatine Monohydrate,
carnitine and ubiquinol (CoQ10). https://b12oils.com/creatine.htm
UNDERSTANDING B12 DEFICIENCY

Annex A:
RnB Treatment Protocol Quick Guide
Start low and slow ISeMo Combo Dosing (Children
Be very consistent with dosing. Spread
& Highly Sensitive)
out your doses across at least two B2/B
Week ISeMo combo oil B12 Oil
applications per day Complex

Start with TINY amounts. Some can 1 1 drop

only handle a pin head.
2 2 drops
Add potassium foods and
supplements as needed when 3 3 drops
adrenaline symptoms start. 4 4 drops
Keep hydrated. No alcohol, no caffeine.
If you experience reactions that last 5 5 drops

more than 4 days without easing up 6 6 drops

and potassium isn’t helping, PLEASE GO
7 7 drops
BACK A LEVEL UNTIL YOU FEEL
COMFORTABLE. 8 8 drops
Go slow and steady, it’s quicker in the 9 Continue @ 8 1mg
long run!
10 Continue @ 8 2mg

This chart uses a week between each 11 Continue @ 8 3mg

increase; however, many people will find
12 Continue @ 8 4mg
that will be much too quick and will have
to increase much slower and at lower 13 Continue @ 8 5mg
doses. 14 Continue @ 8 5mg Pin head

15 Continue @ 8 5mg Pin head x 2

You will know how deficient you are by the
severity of your adrenalin reactions. Slowly increase to
Ongoing Continue @ 8 5mg quarter size over the
coming weeks

Once you are at 8 drops of ISeMo oil, do a

blood test and look at TSH/T4/T3 levels.

TSH has to be as close to 1.0 as you can

get it. Be careful about too much iodine, if
Regularly test TSH/T4/T3
TSH is below 0.5, you need to cut back.
and HTMA levels to ensure
you are activating your B2!!
Once you are on the ISeMo plus B2, then
add the mixed Adenosyl/Methyl B12 oils.
UNDERSTANDING B12 DEFICIENCY

RnB Treatment Protocol Quick Guide Cont'd

Individual Oral Supplements (Adults) Dosing
Iodide (RDA
Selenite (RDA Molyb (RDA B2 / B
Week 150- B12 Oil
200ug/day) 200ug/day) Complex
300ug/day)

1 50

2 100

3 150 25

4 150 50 50 2mg

5 150 100 100 5mg

6 150 150 150 10mg Pin head

7 150 200 200 10mg Pin head x 2

Ongoing 150 200 200 10mg Slowly increase to quarter size

Individual Oils (Adults) Dosing

Note that the GRJ individual oils are 4x strength of the mixed ISeMo oil
Iodide (RDA 150- Selenite (RDA Molyb (RDA B2 / B
Week B12 Oil
300ug/day) 200ug/day) 200ug/day) Complex

1 1/2 drop (37ug)

2 1 drop (75 ug)

3 2 drops (150ug) 1 drop (27.5ug)

4 2 drops (150ug) 2 drops (55ug) 1 drop (50ug) 2mg

5 2 drops (150ug) 3 drops (82.5ug) 2 drops (100ug) 5mg

6 2 drops (150ug) 4 drops (110ug) 3 drops (150ug) 10mg Pin head

7 2 drops (150ug) 5 drops (137.5ug) 4 drops (200ug) 10mg Pin head x 2

8 2 drops (150ug) 6 drops (165ug) 4 drops (200ug) 10mg

Slowly increase to
9 2 drops (150ug) 7 drops (192.5ug) 4 drops (200ug) 10mg
quarter size
Ongoing 2 drops (150ug) 8 drops (220ug) 4 drops (200ug) 10mg