FACULTY OF ALLIED HEALTH SCIENCES

Four Years Undergraduate Program

B.Sc. (Hons. with Research) Biotechnology

Academic Year 2024-25

 TABLE OF CONTENTS

Sl. No. Topic/Content Page No.

1 Nature and extent of the program

2 Program education objective (PEOs)
3 Graduate attributes
4 Qualifications descriptors
5 Program outcomes (POs)
6 Program Specific Outcomes (PSOs)
7 Course structure
8 Semester-wise Course Details
• Semester I
• Semester II
• Semester III
• Semester IV
• Semester V
• Semester VI
• Semester VII
• Semester VIII
9 Mapping of course outcome, program outcomes and program
specific outcomes
10 Annexure
Course Plan
Note: The detailed syllabus for the Semester I and Semester II is incorporated.

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NATURE AND EXTENT OF THE PROGRAM

BSc/B.Sc. (Hons) in Biotechnology program offers a strong foundation for the students in leadership,
research-based and managerial roles in the growing Indian & overseas biotechnology industry. The
program is unique in its approach and provides practical hands-on experience in the area of Biochemistry,
Molecular Biology, Recombinant DNA Technology, Cell Biology, Applied Genetics, Microbiology,
Animal and Plant Tissue Culture, Bioinformatics and Computational Biology, etc. With this blend of
training, graduates can seek employment in research and academia as well as prove their worth in
administrative roles to meet the challenges of the growing Indian economy as well as overseas.

Biotechnology is an essential course for advancing the knowledge of the biotechnology world, addressing
global health challenges, promoting sustainable practices, and driving innovation in various sectors. It has
far-reaching implications for human health, agriculture, industry, and environmental conservation. The
expertise of a Bio-technologist is essential for DNA vaccine which effectively helps in disease control,
drug development, food quality assurance, environmental monitoring, and research advancements. With
the expanding applications of Biotechnology in various sectors, the demand for a professional with
microbiology as a specialization is expected to continue growing. The highly esteemed Bio-technologist
course attracts top-performing students nationwide. The Department of Microbiology, Faculty of Allied
Health Sciences, SGT University offer this course. The comprehensive curriculum comprises theoretical
knowledge and intensive practical work, providing students with a well- rounded understanding of
Biotechnology including immunology, molecular biology, microbiology, plant biotechnology. Under the
NEP program, students have gained significant flexibility. Exiting after the first year earns them an
Undergraduate Certificate in Biotechnology, after the second year an Undergraduate Diploma, after the
third year a Bachelor of Science (Honours) degree in Biotechnology, and upon completion of 176 credits
in the fourth year, a BSc (Honours with Research/Academic Project/Entrepreneurship) degree in
Biotechnology. Our alumni have achieved remarkable success by pursuing higher education at prestigious
institutions and securing promising job placements in other course in Microbiology. This accomplishment
can be attributed to the highly qualified and experienced faculty and this course comes in the Department
of Microbiology, who come from diverse fields within the discipline. The teaching methodology
incorporates lectures, practical, group projects, and interaction with eminent scientists and professionals.
The Department emphasizes independent research projects, industrial/lab visits, and extensive written and
practice-based assignments/review articles/posters by the students, moulding them as independent
researchers.

The University has a well-equipped Biotechnology lab offering a controlled environment with specialized
tools and equipment for enhancing practical skills. It provides the necessary infrastructure and resources
for conducting experiments and facilitates research on a diverse array of topics. Furthermore, several
conferences/lecturers/seminars/trainings are being organized in the department for the overall growth of
the students.

The program is embedded with a choice of Multidisciplinary Generic Electives i.e., outside their core
discipline, along with choice-based value-added courses (VACs), Skill Enhancement Courses (SECs),
Internships, Apprenticeship, Community outreach program, Project (IACP) and Ability Enhancement
Compulsory Courses (AECC) available on the university basket. (Note: This Ordinance shall be aligned
with the Ordinance relating to opting for University Umbrella Courses under NEP 2020). The program

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offers avenues for the students to enter and exit with a certificate, diploma, three- year’ degree or four-
year’ honors degree with research as per the provisions of National Education Policy 2020. This program
is envisaged to be conducted in accordance with Bloom’s Taxonomy pedagogy. The layout of all the
courses in the program is structurally designed with three levels of thinking and each course’s learning
outcomes have been mapped to program outcomes. The course has been designed as per the National
Education Policy guidelines and has some specific features including:

1. Option to exit with a Certificate in Microbiology (total credit = 44, provided submission of proof of any
vocational training received during summer break after end-term exam), Diploma in Microbiology (Total
credit = 88), three-year Degree (total credit = 132), and four years’ honors degree with research (total
Credit = 176) after one-, two-, three- and four-year program completed successfully respectively.

2. The curriculum of each year, with two semesters each, has been designed in such a way that after
completion of each level, the student can gain a certain level of competency with specific academic
components.

3. The emphasis on practical training through meaningfully designed internship courses has been given to
skill development.

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1. PROGRAM EDUCATION OBJECTIVES (PEOs)
The learning objective of B.Sc. (Hons. with Research) Biotechnology Program are:

PEO No. Education Objective

PEO1 Students will develop the practical skills, globally in field of Biotechnology. The
gained knowledge will enable them to pursue the career in research institute,
industry and academia.

PEO2 Graduates will engage in continuous learning and professional development to

prepare a competent generation of Biotechnologists to address challenges in
Diverse sectors like healthcare, agriculture and environmental science.

PEO3 Graduates will be trained in basic and applied molecular biology, immunology,
industrial biotechnology, and bioinformatics to keep abreast of advancements in
Biotechnology and related fields.

PEO4 Students will be able to exhibit leadership and teamwork skills enabling them to
collaborate in multidisciplinary teams and decent career options as entrepreneur or
leadership roles in biotechnology industry.

PEO5 Students will provide sustainable solutions of global problems through research
and innovation capabilities with their expertise fostering a culture of innovation.

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2. GRADUATE ATTRIBUTES

Sl. No. Attributes Description

1 Professional / Graduates will possess a comprehensive understanding of
core concepts, theories, and practical within the field of
Disciplinary
biotechnology.
Knowledge
2 Clinical / technical / Graduates will be proficient in various technical, laboratory,
Laboratory / practical and practical skills relevant to biotechnology, including
skills conducting research based study.
3 Communication Skill Graduates will demonstrate effective verbal and written
communication skills in term of research and skiils in the field
of biotechnology.
4 Cooperation/Team Graduates will be adept at collaborating with teamwork is the
collaborative effort of a group to achieve a common goal or
work
to complete a task in an effective and efficient way.
5 Professional ethics Graduates will develop professional ethics and also gain
knowledge about various ethical issues associated with
biotechnology.
6 Research / Graduates will possess strong research and innovation skills,
including the ability to design and execute research projects,
Innovation-related
critically evaluate existing research and review articles related
Skills to biological field, and contribute to the advancement of
knowledge in the field of biotechnology.
7 Critical thinking and Graduates will demonstrate advanced critical thinking skills,
enabling them to analyze and evaluate in the field of
problem solving
biotechnology theories, practical, as well as to develop
innovative solutions to complex problems with the health and
diseases related.
8 Reflective thinking Graduates will engage in reflective practice, critically
evaluating their own beliefs, assumptions, and professional
practices, and using feedback to enhance their learning and
professional development the concern field.
9 Information/digital Graduates will be proficient in information and digital literacy
literacy skills, including the ability to access, evaluate, and utilize
information effectively and ethically using digital
technologies and resources in the field of biological field
including biotechnology.

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 10 Multi-cultural Graduates will demonstrate cultural sensitivity and
competence competence, recognizing and respecting individual and
cultural diversity in biotechnology theory, research,and
related fields.
11 Leadership Graduates will exhibit leadership readiness and qualities,
readiness/qualities including the ability to inspire and motivate others, facilitate
change, and contribute positively to their profession and
community.
12 Lifelong Learning Graduates will recognize the importance of lifelong learning
and professional development, demonstrating a commitment
to staying abreast of advancements in the field of
biotechnology and continuously improving their knowledge
and skills throughout their careers in related fields.

3. QUALIFICATION DESCRIPTORS:

Qualification descriptors for a BSc Biotechnology (H), 10+2 examination in science or any
equivalent examination in either three subjects amongst Physics, Chemistry, Biology, or
Mathematics or information technology with 50% aggregate marks is prerequisite eligibility
criteria for this program.

➢ Knowledge and Understanding:

• Understanding of basic and applied concepts of Biotechnology and demonstrate interdisciplinary skills
acquired in cell biology, genetics, biochemistry, microbiology, and molecular biology.

• Understanding of basic and applied concepts of Biotechnology and demonstrate interdisciplinary skills
acquired in cell biology, genetics, biochemistry, microbiology, and molecular biology.

• Know the Laboratory skills in cell biology, basic and applied microbiology with an emphasis on
technological aspects.

• Competent to apply the knowledge and skills gained in the fields of Plant biotechnology, animal
biotechnology and microbial technology in pharma, food, agriculture, beverages, herbal and nutraceutical
industries.

• Critically analyze the environmental issues and apply the biotechnology knowledge gained for
conserving the environment and resolving the problems.

• Demonstrate comprehensive innovations and skills in the fields of biomolecules, cell and organelles,
molecular biology, bioprocess engineering and genetic engineering of plants, microbes, and animals with
respect to applications for human welfare.

• Apply knowledge and skills of immunology, bioinformatics, computational modelling of proteins, drug
design and simulations to test the models and aid in drug discovery.

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 • Knowledge to the interpret data, and apply tools of bioinformatics and multi omics in various sectors of
biotechnology including health and Food.

• Demonstrate communication skills, scientific writing, data collection and interpretation abilities in all the
fields of biotechnology.

Conclusions:

• Apply the knowledge of Biotechnology develop the skills of cell biology, molecular biology techniques
and clinical biotechnology.
• Apply the knowledge and application of good laboratory and good manufacturing practices in
biotechnology field.
• Understanding of a animals and plants and demonstrate the ability to identify ethical issues related to
recombinant DNA technology, genetic engineering, intellectual property rights, biosafety, and biohazards.

4. PROGRAM OUTCOMES

PO No. Attribute Competency

PO1 Professional Ability to understand and apply foundational principles and
knowledge theories of Biotechnology. Graduate students will get the
advance knowledge and skills in life science and related fields,
allowing them to function effectively in a variety of biotech
settings.
PO2 Clinical/ Technical Competence in conducting research activities and apply
skills innovative approaches to solving problems related to biotech
field.
PO3 Ethical value & Adherence to ethical principles and codes of conduct in all
professionalism professional interactions and decision-making processes.
PO4 Communication Effective communication skills for establishing rapport,
conducting assessments, and delivering interventions with the
target population.
PO5 Evidence based Ability to critically evaluate research literature and apply
practice/learning evidence-based practices in assessment, intervention, and
evaluation.
PO6 Life-long learning Recognition of the importance of lifelong learning an
professional development for maintaining competence an
adapting to evolving practices and knowledge in biotechnology.
PO7 Entrepreneurship, Capacity for innovation and entrepreneurship in developing and
leadership and implementing career as entrepreneur in biotech settings. Leadership
mentorship skills for guiding and inspiring teams, advocating for the profession, and
fostering collaboration within multidisciplinary settings.

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5. PROGRAM SPECIFIC OUTCOMES

PSO No. Competency

PSO1 To impact and ability to apply Biotechnology skills (including molecular &
biotechnology, immunology, genetic engineering, bioprocess & fermentation, enzyme
& food technology and bioinformatics) and its applications in core and allied fields.
PSO2 To provide students with the concepts and research approaches for their higher career
in the field of biotechnology and develop their scientific interest.
Acquire an ability to identify, formulate, analyze and solve scientific problems in
various areas of biotechnology and allied fields.
PSO3 To impart in-depth practical oriented knowledge to students in various thrust areas of
biotechnology, so as to meet the demands of industry and academia.
Experience opportunity to participate in/manage/curate many co and extracurricular
activities for overall development.

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6. COURSE STRUCTURE
SEMESTER – I

Course Course Title Teaching Credit Marks Distribution

Hours / Week

L T P CL IAE ESE Total

DSC-1 General Microbiology 3 3 40 60 100

DSC-2 General Microbiology Practical 2 1 30 20 50

DSC-3 Biochemistry and metabolism 3 3 40 60 100

DSC-4 Biochemistry Practical 2 1 30 20 50
DSC-5 Chemistry I (Physical and inorganic 3 1 4 40 60 100
chemistry)
GE- 1 4
SEC-1 Biochemical Techniques/ Chemistry Skill 4 2
Lab
AECC- 1 2
VAC- 1 2
Total 22

Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical

Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.

SEMESTER – II

Course Course Title Teaching Credit Marks Distribution

Hours / Week
L T P CL IAE ESE Total
DSC-6 Cell Biology 3 1 4 40 60 100

DSC-7 Genetics 3 3 40 60 100

DSC-8 Genetics Practical 2 1 30 20 50

DSC-9 Chemistry II (organic 3 1 4 40 60 100

chemistry)
GE- 2 4
SEC-2 Organic chemistry skill lab/ cytological 4 2
technique
AECC- 2 2
VAC- 2 2

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 Total 22
Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical
Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.

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SEMESTER – III

Course Course Title Teaching Credit Marks Distribution

Hours / Week
L T P CL IAE ESE Total
DSC-10 Molecular Biology 3 3 40 60 100

DSC-11 Molecular Biology Practical 2 1 30 20 50

DSC-12 Genomics and Proteomics 3 1 4 40 60 100
DSC-13 Food and diary technology 3 1 4 40 60 100

DSE-1/ Bioinstrumentation and 3 1 4 40 60 100

Biotechniques/ Medical
Microbiology/ Plant Diversity

GE – 3
IACP/ 4 2
SEC-3 Critical Research appraisal/ Food
science technology
AECC-3 2
VAC- 3 2
Total 22

Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical

Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.

SEMESTER – IV

Course Course Title Teaching Cre Marks Distribution

Hours / Week dit
L T P CL IAE ESE Total
DSC-14 Recombinant DNA technology 3 1 4 40 60 100

DSC-15 Animal Physiology 3 1 4 40 60 100

DSC-16 Plant Physiology 3 1 4 40 60 100

DSE-2/ Enzymology/Development Biology/Animal 3 1 4 40 60 100
Diversity
GE – 4
IACP/ Technical writing / Genetic Engineering 4 2
SEC-4
AECC-4 2
VAC- 4 2
Total 22
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Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical
Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.

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SEMESTER – V

Course Course Title Teaching Cre Marks Distribution

Hours / Week
dit
L T P CL IAE ESE Total
DSC-17 Immunology 3 1 4 40 60 100
DSC-18 Industrial Technology 3 1 4 40 60 100
DSC-19 Introduction to Bioinformatics 3 3 40 60 100

DSC-20 Bioinformatics Practical 2 1 30 20 50

DSE-3 Bioinstrumentation and 3 1 4 40 60 100

Biotechniques/ Medical
Microbiology/ Plant Diversity

GE- 5 4
IACP/ SEC-5 Minor project/ immunological 4 2
techniques
Total 22

Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical

Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.

SEMESTER – VI

Course Code Course Title Teaching Credit Marks Distribution

Hours / Week
L T P C IAE ES Total
DSC-21 Animal and Plant Biotechnology 3 1 4 40 60 100

DSC-22 Major Project 16 8

DSE-4 Enzymology/Development 3 1 4 40 60 100

Biology/Animal Diversity

GE- 6 4
IACP/ SEC-6 Biotechnological techniques / Biostatistics 4 2

Total 22

Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical

Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.
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SEMESTER –VII
Course Course Title Teaching Cre Marks
Hours / Week dit Distribution
Code
L T P CL IA ESE Total
E
DSC-23 Bioprocess technology 3 1 4 40 60 100
DSE 5 Bioinstrumentation and Biotechniques/ 3 1 4 40 60 100
Medical Microbiology/ Plant Diversity / Drug
Designing/ Bacteriology and virology

DSE 6/ Bioinstrumentation and Biotechniques/ Medical 3 1 4 40 60 100

Microbiology/ Plant Diversity / Drug Designing/
Bacteriology and virology
GE 7
GE 8
Dissertation Minor 10 10

Total 22

Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical

Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester
Examination.

SEMESTER –VIII

Course Course Title Teaching Cred Marks

Hours / Week it Distribution
Code
L T P C IA ESE Total
DSC-24 Biotech in human welfare 3 1 4 40 60 100

GE 9

GE 10
Dissertation Major 18 18

Total 22

Note – L: Lecture Hour/week, T: Tutorial Hour/week, P: Practical Hour/week, CL: Clinical

Hour/week, C: Credits, IAE: Internal Assessment Examination, ESE: End Semester Examination.

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Multidisciplinary Generic Electives (MGE)
Multidisciplinary Generic Electives is credited and choice-based. The students make a choice
from pool of MGE offered by the Faculty under the University. (Reference: University Umbrella
Multidisciplinary Generic Electives)

Value Added Courses (VAC)

Value Added Courses is credited and choice-based. The students make a choice from pool of VAC
offered by the Faculty under the University. (Reference: University Umbrella Value Added
Courses)

Ability Enhancement Compulsory Course (AEC)

Ability Enhancement Compulsory Courses is credited and choice-based. The students make a
choice from pool of AEC offered by the Faculty under the University. (Reference: University
Umbrella Ability Enhancement Compulsory Course)

Skill Enhancement Courses (SEC)

Skill Enhancement Courses is credited and choice-based. The students make a choice from pool
of SEC offered by the Faculty under the University.

Internships, Apprenticeship, Community outreach program, Project (IACP)

Internships, Apprenticeship, Community outreach program, Project is credited and choice-based.
Students can undergo a 4-credit work- based learning/internship/ community service or project
during the summer term.

OVERALL CREDIT DISTRIBUTION TABLE

SEMESTER HOURS PER WEEK Total Credit Marks Distribution
SEMESTER – I L T P TC IAE ESE Total
SEMESTER – II 22
SEMESTER – III 22
SEMESTER – IV 22
SEMESTER – V 22
SEMESTER – VI 22
SEMESTER – VII 22
SEMESTER – VIII 22
Total 22
176

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 Note – L: Lecture Hour, T: Tutorial Hour, P: Practical Hour, TC: Total Credits, IAE: Internal
Assessment Examination, ESE: End Semester Examination.

7. SEMESTER-WISE COURSE DETAILS

SEMESTER-WISE COURSE DETAILS

SEMESTER – I
Course code Course type Course title
DSC-1 General Microbiology
DSC-2 General Microbiology Practical
DSC-3 Biochemistry and Metabolism
DSC-4 Biochemistry Practical
DSC-5 Chemistry I (Physical and Inorganic chemistry)
SEC-1 Biochemical Techniques / Chemistry Skill Lab

Name of the Department Microbiology

Name of the Program B.Sc. (Hons. With research) Biotechnology
Couse Code
Course Title General Microbiology
Academic Year I
Semester I
Number of Credits 3
Course Prerequisite
Course Synopsis The purpose of this course is to introduce the students to the world
of microbiology. Student will gain an understanding of history and
development of microbiology. They will also be able to understand the
classification of different organisms under various kingdoms. Students [-
will be taught the basics characteristics of bacteria, viruses, algae, fungi,
and protozoa. Students will know the life history of the scientists and
their contribution in microbiology and related field. They will gain
proficiency in the principle and applications of important instruments
biological safety cabinets, autoclave, incubator, BOD incubator, hot air
oven, and light microscope. Students will be able to perform sterilization
techniques and they will learn basic staining techniques like simple
staining, Gram’s staining.
Course Outcomes: At the end of the course students will be able to:
CO1 Understand world of microbiology including both beneficial and harmful
microorganisms.
CO2 Know the historical developments in microbiology.
CO3 Articulate different kingdoms of organisms and basic characteristics of
bacteria, viruses, algae, fungi and protozoa.
Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific
Outcomes:

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 PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 2 - - - - 1 - 2 - -

CO2 2 - - - - 1 - - - -

CO3 2 2 - - - 1 - 2 2 1
Avg 2 2 - - - 1 - 2 2 1
1= Weak Correlation 2= Moderate Correlation 3= Strong Correlation
Course Content:
L (Hours/Week) T P (Hours/Week) CL (Hours/Week) Total Hours/Week
(Hours/Week)
3 3
Unit Content & Competency
1 - History of Development of microbiology as a discipline, Spontaneous generation vs.
Development of biogenesis. Development of various microbiological techniques and
Microbiology golden era of microbiology. Establishment of fields of medical
microbiology, immunology, and environmental microbiology with special
reference to the work of following scientists: Anton von Leeuwenhoek,
Joseph Lister, Paul Ehrlich, Edward Jenner, Louis Pasteur, Robert Koch,
Martinus W. Beijerinck, Sergei N. Winogradsky, Alexander Fleming,
Selman A. Waksman, Elie Metchnikoff, Norman Pace, Carl Woese, and
Ananda M. Chakraborty.
2 - Diversity of Microbial A. Systems of classification
World Binomial Nomenclature, Whittaker’s five kingdom and Carl Woese’s
three kingdom classification systems and their utility. Difference between
prokaryotic and eukaryotic microorganisms.
B. General characteristics of different groups: Acellular
microorganisms (Viruses, Viroids, Prions) and Cellular microorganisms
(Bacteria, Algae, Fungi and Protozoa) with emphasis on distribution and
occurrence, morphology, and mode of reproduction.
• Bacteria
Brief introduction to eubacteria and archaebacteria.
• Virus
General characteristics with reference to TMV, T4 and λ phage. Lytic and
lysogenic cycles.
• Fungi
General characteristics of fungi including habitat, distribution, nutritional
requirements, fungal cell ultra-structure, thallus organization and
aggregation, fungal wall structure and synthesis, asexual reproduction,
sexual reproduction, heterokaryosis, heterothallism and parasexual
mechanism. Type studies: Rhizopus, Aspergillus, Saccharomyces and
Agaricus. Economic Importance of Fungi.
•Algae
General characteristics of algae including occurrence, thallus
organization, algae cell ultra-structure, pigments, flagella, eyespot food
reserves and vegetative, asexual and sexual reproduction. Type studies:
Chlamydomonas, Volvox and Spirogyra. Applications of algae.

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 • Protozoa
General characteristics with reference to Amoeba, Giardia, and
Paramecium.

Note: The course plan included as an annexure has the details of each unit with the number of hours and mode of
delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture 41
Practical
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL)/Tutorial
Problem Based Learning (PBL)
Case/Project Based learning (CBL)
Revision 2
Other if any
Total Number of Contact Hours 45

Assessment Methods:
Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce University Examination
Mid Semester Examination
Quiz
Seminars/Presentation Short Answer Questions (SAQ)
Professional Activity Long Answer Questions (LAQ)
Assignment

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2 CO3
Quiz ✓ ✓ ✓
Viva ✓ ✓ ✓
Assignment/Presentation ✓ ✓ ✓
Professional Activity ✓ ✓ ✓
Clinical assessment
Clinical/Practical Log
Book/Record Book
Mid Semester Examination1 ✓ ✓ ✓

Mid Semester Examination 2

University Examination ✓ ✓ ✓

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Feedback process Student’s Feedback

References (List of reference books)

1. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM.T.
Brown Publishers.
2. Black JG. (2008). Microbiology: Principles and Explorations. 7th
edition. Prentice Hall
3. Madigan MT, and Martinko JM. (2006). Brock Biology of Micro-
organisms. 8th edition. Parker J. Prentice Hall International, Inc.
4. Pelczar Jr MJ, Chan ECS, and Krieg NR. (2004). Microbiology.
5th edition Tata McGraw Hill.
5. Srivastava S and Srivastava PS. (2003). Understanding Bacteria.
Kluwer Academic Publishers, Dordrecht
6. Stanier RY, Ingraham JL, Wheelis ML and Painter PR. (2005).
General Microbiology. 5th edition McMillan.
7. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An
Introduction. 9th edition Pearson Education.
8. Willey JM, Sherwood LM, and Woolverton CJ. (2008). Prescott,
Harley and Klein’s Microbiology. 7th edition. McGraw Hill Higher
Education.

Name of the Department of Microbiology

Department
Name of the Program B.Sc. (Hons. With research) Biotechnology
Couse Code
Course Title General Microbiology Practical
Academic Year I
Semester I
Number of Credits 2
Course Prerequisite
Course Synopsis The purpose of this course is to introduce the students to the world
of microbiology. Students will gain proficiency in the principle and
applications of important instruments biological safety cabinets, autoclave,
incubator, BOD incubator, hot air oven, and light microscope. Students will
be able to perform sterilization techniques and they will learn basic staining
techniques like simple staining, Gram’s staining

Course Outcomes: At the end of the course students will be able to:
CO1 Learn the principle and working of various laboratory instruments
CO2 Understand various sterilization methods
CO3 Study algae, protozoan, fungi by mounts

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 Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific
Outcomes:
PO1 PO2 PO3 PO4 PO5 PO6 PSO1 PSO2 PSO3
CO1 2 3 - - 2 - 3 - 3
CO2 2 3 - - 2 - 3 - 3
CO3 2 3 - - 2 - 3 2 3
Avg 2 3 - - 2 - 3 2 3

Course Content:
L T P CL Total Hours/Week
(Hours/Week) (Hours/Week) (Hours/Week) (Hours/Week)
4 2
Unit Content & Competency
Practical Component 1. To study the principle and applications of important instruments
(biological safety cabinets, autoclave, incubator, BOD incubator, hot air
oven, light microscope) used in the microbiology laboratory.
2. Preparation of microbiological medium and its sterilization using
Autoclave.
3. Sterilization of glassware using Hot Air Oven.
4. Sterilization of heat sensitive material by membrane filtration.
5. Simple and Gram’s staining.
6. Study of the following fungi by preparing temporary mounts: Rhizopus and
Aspergillus.
7. Study of the following algae by preparing temporary mounts:
Chlamydomonas and Spirogyra.
8. Study of the following protozoans using permanent mounts/photographs:
Amoeba, Paramecium and Giardia

Note: The course plan included as an annexure has the details of each unit with the number of hours and mode of
delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture 28
Practical
Seminar/Journal Club
Small group discussion (SGD)
Self-directed learning (SDL) / Tutorial
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours
30

Assessment Methods

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 Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce
Mid Semester Examination University Examination
Quiz
Seminars/ Presentation Short Answer Questions (SAQ)
Professional activity Long Answer Question (LAQ)

Assignment

Mapping of Assessment with COs

Nature of Assessment CO1 CO2 CO3
Quiz ✓ ✓ ✓
VIVA ✓ ✓ ✓
Assignment / Presentation ✓ ✓ ✓
Professional Activity ✓ ✓ ✓
Clinical assessment
Clinical/Practical Log Book/ Record ✓ ✓ ✓
Book
Mid Semester Examination 1 ✓ ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓ ✓

Feedback Process 1. Student’s Feedback

References: (List of reference books)

• Dubey RC, Maheshwari DK. (2023). Practical Microbiology(4th
ed). Vikas Publishing House.
• Sastri AS, Bhat S, . Essentials of Practical Microbiology (2nd ed).
Jaypee Publishing House
• Saha R, Das S . Microbiology Practical Manual (2nd ed). CBS
publishers

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Name of the Department Microbiology

Name of the Program B.Sc. (Hons. With research) Biotechnology

Couse Code
Course Title BIOCHEMISTRY & METABOLISM
Academic Year I
Semester I
Number of Credits 3
Course Prerequisite
Course Synopsis Biochemistry is the study of structures and the interactions of
biological macromolecules. These macromolecules include protein,
nucleic acids, lipids, and carbohydrates present in the body
Biochemistry combines biology and chemistry to study living matter.
With biochemistry, we will study chemical reactions at a molecular
level to better understand the world and develop new ways to harness
these. As a result, Biochemistry is being used in research related to
botany, medicine, pharmaceuticals, forensics, nutrition and gene
enhancement. This paper of biochemistry and metabolism will focus
on structure, function and metabolism of various biomolecules
Course Outcomes: At the end of the course students will be able to:
CO1 Understand the carbohydrates and its metabolism
CO2 Having a comprehensive knowledge of amino acids and proteins.

CO3 Having the knowledge of lipids and nucleic acids

Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 3 - - - - 2 - 2 1 -

CO2 3 - - - - 2 - 2 1 -

CO3 3 - - - - 2 - 2 1 -
Avg 3 - - - - 2 - 2 1 -
Course Content:
L (Hours/Week) T (Hours/Week) P (Hours/Week) CL (Hours/Week) Total
Hours/Week
3 3
Unit Content & Competency
1. Carbohydrates Carbohydrates: Definition, function and its classification, Glycosidic
bonds, Glycolysis, Citric acid cycle, gluconeogenesis, glycogen
metabolism, pentose phosphate pathway, Electron Transport Chain,
Oxidative phosphorylation

22
 2. Proteins Proteins: Definition, classification. Amino acids: Definition, structure,
properties, classification, essential & non-essential amino acids. Peptide
bond. Forces stabilizing protein structure and shape. Different Level of
structural organization of proteins, Protein denaturation and renaturation.
Enzyme: definition and function
3. Lipids Lipids: Definition, function and classification. Fatty Acids: nomenclature
and properties,
Phospholipids, sphingolipids, glycolipids, cerebrosides, gangliosides,
Cholesterol.
Fatty acid oxidation and biosynthesis. Ketone body and its metabolism

4. Nucleic acids Nucleic acids: Structure and functions: Physical & chemical properties
of Nucleic acids, Nucleosides & Nucleotides, purines & pyrimidines,
Double helical model of DNA structure, denaturation and renaturation
of DNA, De novo and salvage pathway for purine and pyrimidine
synthesis

Note: The course plan included as an annexure has the details of each unit with the number of hours and mode of
delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture 41
Practical
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL)/Tutorial
Problem Based Learning (PBL)
Case/Project Based learning (CBL)
Revision 2
Other if any
Total Number of Contact Hours 45

Assessment Methods:
Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce
Mid Semester Examination University Examination
Quiz
Seminars/Presentation
Professional Activity Short Answer Questions (SAQ)
Assignment Long Answer Questions (LAQ)

23
Mapping of Assessment with Cos
Nature of Assessment CO1 CO2 CO3
Quiz ✓ ✓ ✓
Viva ✓ ✓ ✓
Assignment/Presentation ✓ ✓ ✓
Professional Activity ✓ ✓ ✓
Clinical assessment
Clinical/Practical Log
Book/Record Book
Mid Semester Examination1 ✓ ✓ ✓
Mid Semester Examination 2

University Examination ✓ ✓ ✓

Feedback process Student’s Feedback

References (List of reference books)

• Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006).
Biochemistry. VI Edition. W.H Freeman and Co.
• Nelson, D.L., Cox, M.M. (2017) Lehninger
Principles of Biochemistry, 17th Edition, WH
Freeman and Company, New York, USA.
• Vasudevan D.M.,, Sreekumari S, Vaidhyanathan K.
(2011) Textbook of Biochemistry for medical
students, 7th Edition, Jaypee Publishers.
• Satyanarayana U, Chakrapani U (2021)
Biochemistry, 6th Edition Elsevier

Name of the Department of Microbiology

Department
Name of the Program B.Sc. (Hons. with Research) in Biotechnology
Couse Code
Course Title Biochemistry Practical
Academic Year I
Semester I
Number of Credits 1
Course Prerequisite
Course Synopsis Basic Biochemistry is a foundational course designed for students enrolled in
the Bachelor of Science in biotechnology program. In this paper, students will
learn basic biochemistry practical pertaining to qualitative detection of various
biomolecules. By the end of the course, students will be equipped with the
knowledge and skills necessary to identify the biomolecules in the various
samples.

24
 Course Outcomes: At the end of the course students will be able to:
CO1 Enumerate Different types of carbohydrate identification tests
CO2 Identify protein and nucleic acids by different tests

Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific

Outcomes:
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 2 3 - - - 1 - 3 - 3

CO2 2 3 - - - 1 - 3 - 3
Avg 2 3 - - - 1 - 3 - 3
Course Content:
L (Hours/Week) T P CL Total Hours/Week
(Hours/Week) (Hours/Week) (Hours/Week)
4 4
Unit Content & Competency
Practical Component 1. Identification of carbohydrates by Molisch’s test.
2. Identification of Reducing sugar by Benedict’s test.
3. Identification of ketose sugars by Seliwanoff’s test.
4. Identification of reducing sugar by Osazone test.
5. Identification of cholesterol by Salkowski’s test.
6. Identification of protein by Biuret’s test.
7. Identification of protein by Ninhydrin test.
8. Identification of DNA by Dische diphenylamine test
9. Identification of RNA by Orcinol test

Note: The course plan included as an annexure has the details of each unit with the number of hours and mode of
delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Practical 28
Seminar/Journal Club
Small group discussion (SGD)
Self-directed learning (SDL) / Tutorial
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours 30

25
Assessment Methods:
Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce
Mid Term Practical Examination University Examination
Quiz
Seminars/Presentation Practical Examination & Viva-voce
Professional Activity
Assignment

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2
Quiz ✓ ✓
Viva ✓ ✓
Assignment/Presentation ✓ ✓
Professional Activity ✓ ✓
Clinical assessment
Clinical/Practical Log ✓ ✓
Book/Record Book
Mid Semester Examination1 ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓

Feedback Process 1. Student’s Feedback

References (List of reference books)
Gupta, S.K. (2023) Manual of Practical Biochemistry. (5th ed) Arya
Publishing Company.

Name of the Department Microbiology

Name of the Program B.Sc. (Hons. With research) Biotechnology
Couse Code
Course Title Chemistry I (Physical and Inorganic chemistry)
Academic Year I
Semester I
Number of Credits 4
Course Prerequisite
Course Synopsis The purpose of this course is to introduce the student to the basics
of physical and inorganic chemistry. Student will gain an understanding
of acid-base equilibria, chemical kinetics, and chemical thermodynamics.
They will gain insight into different bonds exists between molecules, and

26
 atomic structure. Apart from this the students will understand the
chemistry related to life and biological forms. This course will equip the
students with the necessary chemical knowledge concerning the
fundamentals in the basic areas of physical and inorganic chemistry.
Course Outcomes: At the end of the course students will be able to:
CO1 Understand the basics of physical and inorganic chemistry.
CO2 Learn acid-base equilibria, chemical kinetics, and chemical
thermodynamics.
CO3 Learn about atomic structure, and ionic and covalent bonding.
CO4 Interpret chemistry related to life and biological forms.
Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific Outcomes:
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3
CO1 2 - - - - 2 - 2 1 -

CO2 2 2 - - - 2 - 2 1 -

CO3 2 - - - - 2 - 2 1 -

CO4 2 - - - - 2 - 2 1 -
Avg 2 2 - - - 2 - 2 1 -
Course Content:
L (Hours/Week) T P (Hours/Week) CL (Hours/Week) Total Hours/Week
(Hours/Week)
3 1 4
Unit Content & Competencies
1 - Physical Chemistry A. Acids-Bases and Ionic Equilibria
Modern concepts of acids and bases: Arrhenius theory, Bronsted and
Lowry’s concept, Lewis concept with typical examples, applications and
limitations. Strengths of acids and bases (elementary idea). Ionization of
weak acids and bases in aqueous solution, ionization constants, Strong,
moderate and weak electrolytes, degree of ionization, factors affecting
degree of ionization. pH scale, common ion effect.
B. Chemicals Kinetics: Rate, order and molecularity of a reaction, rate
constants of first and second order reactions, half life period, influence of
temperature on reaction rate, activation energy, determination of order of a
reaction.
C. Chemical Thermodynamics: State functions, thermodynamic
equilibrium, work, heat, internal energy, enthalpy. First Law of
Thermodynamics. Reversible isothermal and adiabatic
expansion/compression of an ideal gas. Irreversible isothermal and adiabatic
expansion. Enthalpy change and its measurement, standard heats of
formation and absolute enthalpies. Kirchhoff’s equation. Second and Third
Law: Various statements of the second law of thermodynamics. Efficiency of
a cyclic process (Carnot’s cycle), Entropy, Entropy changes of an ideal gas
with changes in P, V, and T. Free energy and work functions, Gibbs-
Helmholtz Equation. Criteria of spontaneity in terms of changes in free
energy, Third law of thermodynamics, Absolute entropies.

27
 2 - Inorganic Chemistry A. Atomic structure: Wave mechanical model of Hydrogen atom, The de
Broglie relationship, The uncertainty principle, Schrodinger wave
equation and its derivation, Significance of Ψ and Ψ2, Quantum numbers,
Normal and orthogonal wave functions, Pauli’s exclusion principle,
Hund’s rule of maximum multiplicity, Aufbau principle and its
limitations. Concept of extra stability of half and completely filled
electronic configuration.
B. Ionic Bonding: General characteristics of ionic bonding. Energy
considerations in ionic bonding, lattice energy and salvation energy and
their importance in the context of stability and solubility of ionic
compounds. Polarizing power and polarizability. Fajan’s rules, ionic
character in covalent compounds, bond moment, dipole moment and
percentage ionic character.
C. Covalent bonding: VB Approach Shapes of some inorganic molecules
and ions on the basis of VSEPR and hybridization with suitable examples
of, linear, trigonal planar, square planar, tetrahedral, trigonal bipyramidal
and octahedral arrangements. Hydrogen bonding and its effect of physical
and chemical properties. Concept of resonance and resonating structures.

Note: The course plan included as an annexure has the details of each unit with the number of hours and mode of
delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture 41
Practical
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL)/Tutorial 15
Problem Based Learning (PBL)
Case/Project Based learning (CBL)
Revision 2
Other if any
Total Number of Contact Hours 60

Assessment Methods:
Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce University Examination
Mid Semester Examination Short Answer Questions (SAQ)
Quiz Long Answer Questions (LAQ)
Seminars/Presentation
Professional Activity
Assignment

28
Mapping of Assessment with Cos
Nature of Assessment CO1 CO2 CO3 CO4
Quiz ✓ ✓ ✓ ✓
Viva ✓ ✓ ✓ ✓
Assignment/Presentation ✓ ✓ ✓ ✓
Professional Activity ✓ ✓ ✓ ✓
Clinical assessment
Clinical/Practical Log
Book/Record Book
Mid Semester ✓ ✓ ✓ ✓
Examination1
Mid Semester
Examination 2
University Examination ✓ ✓ ✓ ✓

Feedback process Student’s Feedback

References (List of reference books)

1. Barrow GM. (2007). Physical Chemistry. Tata McGraw‐Hill.
2. Castellan GW. (2004). Physical Chemistry. 4th edition.
Narosa.
3. Cotton FA and Wilkinson G. (Year). Basic Inorganic
Chemistry. John Wiley.
4. Douglas, McDaniel and Alexader. (Year). Concepts and
Models in Inorganic Chemistry. John Wiley.
5. Huheey JE, Keiter E and Keiter R. (Year). Inorganic
Chemistry: Principles of Structure and Reactivity. Pearson
Publication.
6. Kotz JC, Treichel PM and Townsend JR. (2009). General
Chemistry. Cengage Learning India Pvt. Ltd., New Delhi.
7. Lee JD. (Year). A New Concise Inorganic Chemistry, E L. B.
S.
8. Mahan BH. (1998). University Chemistry. 3rd edition.
Narosa
9. Vogel A.I. Vogel’s Qualitative Inorganic Analysis. 7th
edition. Prentice Hall
10. Vogel A.I. Vogel’s Quantitative Chemical Analysis. 6th
edition. Prentice Hall.

29
Name of the Microbiology
Department
Name of the Program B.Sc. (Hons. With research) Biotechnology
Couse Code
Course Title BIOCHEMICAL TECHNIQUES
Academic Year I
Semester I
Number of Credits 2
Course Prerequisite
Course Synopsis Biochemistry is the study of structures and the interactions of
biological macromolecules. These macromolecules include
protein, nucleic acids, lipids, and carbohydrates present in the
body Biochemistry combines biology and chemistry to study
living matter. With biochemistry, we will study chemical
reactions at a molecular level to better understand the world and
develop new ways to harness these. As a result, Biochemistry is
being used in research related to botany, medicine,
pharmaceuticals, forensics, nutrition and gene enhancement. This
paper of biochemical techniques will focus on various techniques
which are useful in the field of biochemistry
Course Outcomes: At the end of the course students will be able to:
CO1 Having a comprehensive knowledge of spectrophotometric and
electrophoretic methods
CO2 Having a comprehensive knowledge of chromatography
Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific
Outcomes:
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3
CO1 3 3 - - - 2 - 3 - 3
CO2 3 3 - - - 2 - 3 - 3
Avg 3 3 - - - 2 - 3 - 3
Course Content:
L T P CL Total
(Hours/Week) (Hours/Week) (Hours/Week) (Hours/Week) Hours/Week
4 4
Unit Content & Competency
Practical Component 1.Quantitative estimation of DNA and protein by using
spectrophotometry
2. Validation of Lambert - Beer’s law and derivation of standard
curve in colorimetry and Determination of unknown concentration
of coloured solutions by photometric method.
3. Quantitative estimation of DNA & Quality check of DNA by
agarose gel electrophoresis
4. Polyacrylamide gel electrophoresis of proteins.

30
 5. Separation of analytes by performing paper & thin layer
chromatography

Note: The course plan included as an annexure has the details of each unit with the number of hours and mode of
delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture
Practical 26
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours
30

Assessment Methods:
Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce
Mid Term Practical Examination University Examination
Quiz Short Answer Questions (SAQ)
Seminars/Presentation Long Answer Questions (LAQ)
Professional Activity Practical Examination & Viva-voce
Assignment

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2
Quiz ✓ ✓
Viva ✓ ✓
Assignment/Presentation ✓ ✓
Professional Activity ✓ ✓
Clinical assessment
Clinical/Practical Log Book/Record ✓ ✓
Book
Mid Semester Examination1 ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓

31
Name of the Microbiology
Department
Name of the Program B.Sc. (Hons. With research) Biotechnology
Couse Code
Course Title Chemistry skill Laboratory
Academic Year I
Semester I
Number of Credits 2
Course Prerequisite
Course Synopsis The purpose of this course is to introduce the student to the basics
of physical and inorganic chemistry. Student will gain an understanding of
acid-base equilibria, chemical kinetics, and chemical thermodynamics. They
will gain insight into different bonds exists between molecules, and atomic
structure. Apart from this the students will understand the chemistry related to
life and biological forms. This course will equip the students with the necessary
chemical knowledge concerning the fundamentals in the basic areas of physical
and inorganic chemistry
Course Outcomes: At the end of the course students will be able to:
CO1 Having a comprehensive knowledge of measurement of physical
parameters
CO2 Having a comprehensive knowledge of volumetric analysis and titration
Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific
Outcomes:
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3
CO1 3 3 2 - - 1 - 1 - 2
CO2 3 3 2 - - 1 - 1 - 2
Avg 3 3 2 - - 1 - 1 - 2
Course Content:
L T P (Hours/Week) CL (Hours/Week) Total Hours/Week
(Hours/Week) (Hours/Week)
4 4
Unit Content & Competencies
Practical Component Section A: Physical Chemistry
Surface tension measurement (use of organic solvents excluded)
Determination of the surface tension of a liquid or a dilute solution using a
stalagmometer.
Viscosity measurement (use of organic solvents excluded)
Determination of the relative and absolute viscosity of a liquid or dilute
solution using an Ostwald’s viscometer.
Kinetic studies:
To study the specific reaction rate of the acid-catalyzed hydrolysis of methyl
acetate at room temperature.
Section B: Inorganic Chemistry
Volumetric Analysis: any 5

32
 1. Estimation of a strong acid (HCl) by titrating it with standardized NaOH.
2. Estimation of oxalic acid by titrating it with KMnO4.
3. Estimation of Fe(II) ions by titrating it with K2Cr2O7 using internal
indicator.
4. Estimation of Cu(II) ions iodometrically using Na2S2O3.
5. Estimation of (i) Mg2+ or (ii) Zn2+ by complexometric titrations using
EDTA.
6. Estimation of sodium carbonate and sodium hydrogen carbonate present in
a mixture.
7. Estimation of water of crystallization in Mohr’s salt by titrating with KMnO4.

Note: The course plan included as an annexure has the details of each unit with the number of hours
and mode of delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture
Practical 26
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours
30

Assessment Methods:
Formative Summative
Multiple Choice Questions (MCQ)
Viva-voce
Mid Term Practical Examination University Examination
Quiz Short Answer Questions (SAQ)
Seminars/Presentation Long Answer Questions (LAQ)
Professional Activity Practical Examination & Viva-voce
Assignment

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2
Quiz ✓ ✓
Viva ✓ ✓

33
Assignment/Presentation ✓ ✓
Professional Activity ✓ ✓
Clinical assessment
Clinical/Practical Log Book/Record ✓ ✓
Book
Mid Semester Examination1 ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓

34
 SEMESTER – II

Course Code Course Type Course Title

DSC-6 Cell Biology
DSC-7 Genetics
DSC-8 Genetics Practical
DSC-6 Chemistry II

Name of the Department Department of Microbiology

Name of the Program B.Sc. (Hons. with Research) Biotechnology
Course Code
Course Title CELL BIOLOGY
Academic Year I
Semester II
Number of Credits 4
Course Prerequisite
Course Synopsis Cell biology typically covers the fundamental principles of cell
structure, function, and organization. Topics may include cell
membrane structure and function, organelles, cell signaling, cell
cycle regulation, DNA replication, gene expression, and cellular
metabolism. Additionally, discussions may extend to topics such as
cell communication, cellular transport mechanisms, cell division,
and the role of cells in development, physiology, and disease.
Practical applications and current research in cell biology are also
often explored.
Course Outcomes:
At the end of the course students will be able to:
CO1 The student will about the various component of cell; cellular organelles and their roles
and structure and organization of cytoskeleton
CO2 The students will learn about the molecular organization of chromatin and structure and
organization of nuclear envelope, nuclear pore complex and nuclear lamina.

35
CO3 Articulate the protein sorting and transport from the Endoplasmic reticulum and golgi
apparatus to various cell structure

CO4 Analyzing cell signaling pathways their communication and cell cycle, cell death

CO5 On the completion of the course, students shall develop understanding of basics
concepts of cell biology and students were capable to apply the knowledge of
cell biology to research or practical applications.

Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific Outcomes:

Cos PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 3 - - - - 2 - 3 2 -

CO2 3 - - - - 2 - 3 2 -

CO3 3 - - - - 2 - 3 2 -

CO4 3 - - - - 2 - 3 2 -

CO5 3 - - - - 2 - 3 2 -

Average 3 - - - - 2 - 3 2 -

1= Weak Correlation 2= Moderate Correlation 3=Strong Correlation

Course Content:
L (Hours/Week) T (Hours/Week) P (Hours/Week) CL Total
(Hours/Week) Hour/Week
3 1 - 4
Unit Content & Competencies
1. Structure of Plasma membrane: Structure and transport of small molecules. Cell Wall: Eukaryotic cell
Cell wall, Extracellular matrix and cell matrix interactions, Cell-Cell Interactions - adhesion
junctions, tight junctions, gap junctions, and plasmodesmata (only structural aspects).
Mitochondria, chloroplasts and peroxisomes. Cytoskeleton: Structure and organization of
actin filaments, association of actin filaments with plasma membrane, cell surface
protrusions, intermediate filaments, microtubules.

36
 2. Nucleus Nuclear envelope, nuclear pore complex and nuclear lamina.
Chromatin – Molecular organization.
Nucleolus.
3. Endoplasmic Reticulum – Structure, targeting and insertion of proteins in the ER, protein
Protein Sorting folding, processing and quality control in ER, smooth ER and lipid synthesis, export of
and Transport
proteins and lipids Golgi Apparatus – Organization, protein glycosylation, protein sorting
and export from Golgi Apparatus. Lysosomes.
4. Signalling molecules and their receptors.
Cell Signalling
Function of cell surface receptors.
Pathways of intracellular receptors – Cyclic AMP pathway, cyclic GMP and MAP kinase
pathway.

5.Cell Cycle, Eukaryotic cell cycle and its regulation, Mitosis and Meiosis.
Cell Death and
Development of cancer, causes, types, Diagnosis and therapy. Programmed cell death.
Cell Renewal

Note: The course plan included as an annexure has the details of each unit with the number ofhours
and mode of delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours

Lecture 41
Practical
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial 15
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours 60

37
Assessment Methods:

Formative Summative
Viva-voce
Mid Semester Examination University Examination
Quiz Short Answer Questions (SAQ)
Seminars/ Presentation Long Answer Question (LAQ)
Professional Activity
Assignment

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2 CO3 CO4 CO5

Quiz ✓ ✓ ✓ ✓ ✓
VIVA ✓ ✓ ✓ ✓ ✓
Assignment / Presentation ✓ ✓ ✓ ✓ ✓
Professional Activity ✓ ✓ ✓ ✓ ✓
Clinical assessment
Clinical/Practical Log Book/ Record Book
Mid Semester Examination 1 ✓ ✓ ✓ ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓ ✓ ✓ ✓

Feedback Process 5. Student’s Feedback

References: (List of reference books)

38
 • Hardin J, Bertoni G and Kleinsmith LJ. (2010). Becker’s World of the Cell.
8th edition. Pearson.
• Karp G. (2010) Cell and Molecular Biology: Concepts and Experiments.
6th edition. John Wiley & Sons. Inc.
• De Robertis, EDP and De Robertis EMF. (2006). Cell and Molecular
Biology. 8th edition. Lipincott Williams and Wilkins, Philadelphia.
• Cooper, G.M. and Hausman, R.E. (2009). The Cell: A Molecular
Approach. 5th Edition. ASM Press & Sunderland, Washington, D.C.;
Sinauer Associates, MA.

Name of the Department Microbiology

Name of the Program B.Sc. (Hons. with Research) Biotechnology
Course Code

Course Title Genetics

Academic Year I
Semester II
Number of Credits 3
Course Prerequisite
Course Synopsis The course provides a comprehensive overview of genetic principles,
applications, central dogma, and molecular genetics. Students will gain
experience in analyzing and interpreting data from genetics experiments. In
addition, students will be able to learn how model organisms can be used for
understanding genetics principles and specific biological processes. It also
provides an opportunity for students to search through the specific topics in
genetics through research projects and laboratory exercises. Students will
learn how advances in our understanding of genetics and molecular biology
have impacted society.
Course Outcomes:
At the end of the course students will be able to:
CO1 Overview of Mendelian and Non-Mendelian Inheritance, historical perspectives and
extensions to Mendelian genetics.

CO2 Study of Linkage and crossing over, molecular mechanism of crossing over, chromosomal
mutations and gene mutations.

39
 CO3 To know the concept of chromosomal abnormalities, mechanism of sex determination and sex
linkage and the role of environment in determining the sex.
CO4 Enhance the knowledge of extra-nuclear inheritance, evolutionary and population genetics

CO5 Having the comprehensive knowledge of cell cycle, karyotyping and pedigree analysis

Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO
3
CO1 3 - - - - 2 - 3 2 -

CO2 3 - - - - 2 - 3 2 -

CO3 3 - - - - 2 - 3 2 -

CO4 3 - - - - 2 - 3 2 -

CO5 3 - - - - 2 - 3 2 -

Average 3 - - - - 2 - 3 2 -

1= Weak Correlation 2= Moderate Correlation 3= Strong Correlation

Course Content:

L (Hours/Week) T (Hours/Week) P CL Total

(Hours/Week) (Hours/Week) Hour/Week
3 1 4
Unit Content & Competencies
1 Mendelian genetics: Mendel’s experimental design, monohybrid, di-hybrid and tri hybrid
Introduction crosses, Law of segregation & Principle of independent assortment. Concept of gene: Allele,
to Genetics multiple alleles, pseudoallele, pleiotropy, essential and lethal genes, penetrance and
expressivity. Non allelic interactions: Interaction producing new phenotype complementary
genes, and epistasis (dominant & recessive).

2 Linkage and crossing over, Cytological basis of crossing over, Molecular mechanism of
Linkage, crossing over, Recombination frequency as a measure of linkage intensity, two factor and
Crossing over three factor crosses, Interference and coincidence, Somatic cell genetics – an alternative
and Mutations approach to gene mapping. Chromosomal Mutations: Deletion, Duplication, Inversion,
Translocation, Aneuploidy and Polyploidy; Gene mutations: Induced versus Spontaneous
mutations, Back versus Suppressor mutations, Molecular basis of Mutations in relation to UV
light and chemical mutagens.

40
 3- Chromosomal abnormalities– Aneuploidy and Euploidy. Sex determination and sex linkage:
Chromosoma Mechanisms of sex determination, Environmental factors and sex determination, sex
l aberrations differentiation, Barr bodies, dosage compensation, genetic balance theory, Fragile-X-
and sex syndrome and chromosome, sex influenced dominance, sex limited gene expression, sex
determinatio linked inheritance.
n

4– Inheritance of mitochondrial and chloroplast genes, maternal inheritance. Evolution and

Extra population genetics: In breeding and out breeding, Hardy Weinberg law (prediction,
chromosomal derivation), allelic and genotype frequencies, changes in allelic frequencies, systems of
inheritance mating, natural selection.
and
population
genetics

Note: The course plan included as an annexure has the details of each unit with the number of
hours and mode of delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours

Lecture 41
Practical
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial 15
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours 60

Assessment Methods:

Formative Summative
Multiple Choice Questions (MCQ) University Examination
Viva-voce Short Answer Questions (SAQ)
Mid Semester Examination Long Answer Question (LAQ)

41
 Quiz
Seminars/ Presentation
Professional Activity
Assignment

Mapping of Assessment with COs

Nature of Assessment CO1 CO2 CO3 CO4 CO5

Quiz ✓ ✓ ✓ ✓ ✓
VIVA ✓ ✓ ✓ ✓ ✓
Assignment / Presentation ✓ ✓ ✓ ✓ ✓
Professional Activity ✓ ✓ ✓ ✓ ✓
Clinical assessment
Clinical/Practical Log Book/ Record
Book
Mid Semester Examination 1 ✓ ✓ ✓ ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓ ✓ ✓ ✓

Feedback Process 1. Student’s Feedback

References: (List of reference books)

• Griffiths, A. J. (2005). An introduction to genetic analysis.

Macmillan.

42
 • Sturtevant, A. H., & Beadle, G. W. (1939). An introduction to genetics.
• Griffiths, A. J. (2002). Modern genetic analysis: integrating genes and
genomes (Vol. 1). Macmillan.
• Snustad, D. P., & Simmons, M. J. (2015). Principles of genetics. John
Wiley & Sons.

• https://academicworks.cuny.edu/cgi/viewcontent.cgi?article=1008&cont
ext=ny_oers, (BIO2450L-Genetics; Prof. Christopher Blair)

Course Title Genetics practical

Academic Year I
Semester II
Number of Credits 1
Course Prerequisite
Course Synopsis The course provides a hands on experience with genetic analysis and
molecular biology tools. Students will gain experience in analyzing and
interpreting data from genetics experiments. In addition, students will be able
to learn how model organisms can be used for understanding genetics
principles and specific biological processes. It also provides an opportunity
for students to learn the methods and principles used in the genetics, both the
classical and molecular approaches.
Course Outcomes:
At the end of the course students will be able to:
CO1 Understand the basic techniques in genetics and molecular biology, Analyze and interpret
genetic data

CO2 To gain practical experience with laboratory equipment’s and protocols and enhance the
knowledge of extra-nuclear inheritance, evolutionary and population genetics
Mapping of Course Outcomes (COs) to Program Outcomes (POs)& Program Specific Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 2 2 1 - - 2 2 3 3 3

CO2 2 2 1 - - 2 2 3 3 3

43
Average 2 2 1 - - 2 2 3 3 3

1= Weak Correlation 2= Moderate Correlation 3= Strong Correlation

Course Content:

L (Hours/Week) T (Hours/Week) P CL Total

(Hours/Week) (Hours/Week) Hour/Week
4 4
Unit Content & Competencies
1. Permanent To understand the process of mitosis, hands on experience with microscope handling,
and temporary preparing slides, and analyzing cell division stages.
mount of
mitosis.

2. Permanent To understand the process of meiosis, hands on experience with microscope handling,
and preparing slides, and analyzing cell division stages.
temporary
mount of
meiosis.

44
 3. Mendelian The students can understand complex genetic patterns that deviate from Mendelian genetics.
deviations in The students can explore various types of deviations from classical Mendelian genetics
dihybrid crosses
4. Karyotyping with It will help the students to study principles and techniques used to study chromosomes.
the help of It focuses on identifying chromosomal structures, preparing karyotypes.
photographs
5. Pedigree charts of Hands on experience in analyzing pedigree charts to study the pattern of Inheritance
some common of traits or diseases. It will help them to understand how traits are passed through
characters like blood generations.
group, color
blindness
6. Study of The understanding of polyploidy, its impact on the traits, and experiments to induce
polyploidy in onion polyploidy.
root tip by
colchicine treatment

Note: The course plan included as an annexure has the details of each unit with the number of
hours and mode of delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours

Lecture 2
Practical 26
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision
Others If any:
Total Number of Contact Hours 30

Assessment Methods:

Formative Summative
Multiple Choice Questions (MCQ) University Examination
Viva-voce Multiple Choice Questions (MCQ)
Mid Term Practical Examination Short Answer Questions (SAQ)

45
 Quiz Long Answer Question (LAQ)
Seminars/ Presentation Practical Examination & Viva-voce
Professional Activity

Mapping of Assessment with COs

Nature of Assessment CO1 CO2

Quiz ✓ ✓
VIVA ✓ ✓
Assignment / Presentation ✓ ✓
Professional Activity ✓ ✓
Clinical assessment
Clinical/Practical Log Book/ Record ✓ ✓
Book
Mid Semester Examination 1 ✓ ✓
Mid Semester Examination 2
University Examination ✓ ✓

Feedback Process 1. Student’s Feedback

References: (List of reference books)

• https://jru.edu.in/studentcorner/lab-
manual/agriculture/Fundamentals%20of%20Genetics.pdf
• https://academicworks.cuny.edu/cgi/viewcontent.cgi?article
=1008&context=ny_oers

46
• researchgate.net/profile/Worku-Mhiret-
2/publication/339127895_Laboratory_Manual_for_Principles_of_Genet
ics/links/5e3ee1d8299bf1cdb918e9da/Laboratory-Manual-for-
Principles-of-Genetics.pdf

47
Name of the Department of Microbiology
Department
Name of the Program B.Sc. (Hons. with Research) Biotechnology
Course Code
Course Title CHEMISTRY II (ORGANIC CHEMISTRY)
Academic Year I
Semester II
Number of Credits 4
Course Prerequisite
Course Synopsis A course synopsis of organic chemistry typically delves into the study of
carbon-containing compounds, their structure, properties, reactions, and
synthesis. Topics covered often include the structure and bonding of organic
molecules, functional groups, stereochemistry, reaction mechanisms,
spectroscopic methods for structural determination, and the principles of
organic synthesis. Additionally, discussions may extend to topics such as
aromaticity, the relationship between structure and reactivity in organic
molecules. Practical applications in fields such as pharmaceuticals,
materials science, and biochemistry are also often explored.
Course Outcomes:
At the end of the course students will be able to:
CO1 Understanding the fundamental principles and theories of organic chemistry, including
bonding, structure and reactivity
CO2 Demonstrating proficiency in naming and drawing organic compounds, including
functional groups and isomerism.

CO3 Applying principles of organic chemistry to predict the outcomes of chemical reactions,
including mechanisms and stereochemistry.

CO4 On the completion of the course, students shall develop understanding of basic role of
organic chemistry in the synthesis of pharmaceuticals, materials and other applications as
well as related fields like biochemistry and research.
Mapping of Course Outcomes (COs) to Program Outcomes (POs) & Program Specific
Outcomes:
COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 2 - - - - 2 1 2 2 2

48
CO2 2 - - - - 2 1 2 2 2
CO3 2 - - - - 2 1 2 2 2
CO4 2 - - - - 2 1 2 2 2
Avg 2 - - - - 2 1 2 2 2
1= Weak Correlation 2= Moderate Correlation 3= Strong Correlation

Course Content:
L (Hours/Week) T (Hours/Week) P (Hours/Week) Total Hour/Week
3 1 4
Unit Content & Competencies
1. Recapitulation Hybridization, Shapes of molecules
of basics of Electronic Displacements: Inductive, electromeric, resonance and mesomeric
Organic effects, hyperconjugation Dipole moment; Hydrogen bonding (Applications to be
Chemistry discussed with relevant topics)
Homolytic and Heterolytic fission with suitable examples. Curly arrow rules,
formal charges; Electrophiles and Nucleophiles; Types, shape and relative
stability of Carbocations, Carbanions, Free radicals and Carbenes.
Introduction to types of organic reactions: Addition, Elimination and Substitution
reactions
2. Stereochemistry Fischer, Newmann and Sawhorse Projection formulae and their interconversions;
Geometrical isomerism: cis–trans , syn-anti and E/Z notations with C.I.P rules.

Optical Isomerism: Optical Activity, Specific Rotation, Chirality/Asymmetry,

Enantiomers, Molecules with two or more chiral-centres, Distereoisomers, meso
structures, Racemic mixture and their resolution. Relative and absolute
configuration: D/L and R/S designations.

49
3. Chemistry of General methods of prepareation , physical and chemical properties of alkanes:
Aliphatic Wurtz Reaction,Wurtz-Fittig Reactions, Free radical substitutions: Halogenation-
Hydrocarbons: relative reactivity and selectivity.

A. Carbon-Carbon
sigma bonds

B. Carbon-Carbon pi General methods of preparation, physical and chemical properties of alkenes and
bonds: alkynes, Mechanism of E1, E2, E1cb reactions. Saytzeff and Hofmann eliminations.
Electrophilic additions their mechanisms (Markownikoff/ Anti Markownikoff
addition), mechanism of oxymercuration-demercuration, hydroboration- oxidation,
ozonolysis, reduction (catalytic and chemical), syn and anti-
hydroxylation(oxidation). 1,2-and 1,4-addition reactions in conjugated dienes and
Diels-Alder reaction; Allylic and benzylic bromination and mechanism, e.g.
propene, 1-butene, toluene, ethyl benzene.

Reactions of alkynes: Acidity, Electrophilic and Nucleophilic additions. Hydration

to form carbonyl compounds, Alkylation of terminal alkynes.

4.Aromatic Aromaticity: Hückel’s rule, aromatic character of arenes, cyclic

Hydrocarbons carbocations/carbanions and heterocyclic compounds with suitable examples.
Electrophilic aromatic substitution: halogenation, nitration, sulphonation and
Friedel-Craft’s alkylation/acylation with their mechanism. Directing effects of the
groups.

50
 Note: The course plan included as an annexure has the details of each unit
with the number ofhours and mode of delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture 41
Practical
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial 15
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision 2
Others If any:
Total Number of Contact Hours 60

Assessment Methods:

Formative Summative
Viva-voce University Examination
Mid Semester Examination Short Answer Questions (SAQ)
Quiz Long Answer Question (LAQ)
Seminars/ Presentation
Professional Activity
Assignment

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2 CO3 CO4

Quiz ✓ ✓ ✓ ✓
VIVA ✓ ✓ ✓ ✓
Assignment / Presentation ✓ ✓ ✓ ✓
Professional Activity ✓ ✓ ✓ ✓
Practical Log Book / Record Book
Mid Semester Examination 1 ✓ ✓ ✓ ✓ 51
 Mid Semester Examination 2

University Examination ✓ ✓ ✓ ✓

Feedback Process 6. Student’s Feedback

References: (List of reference books)

• Morrison, R. N. & Boyd, R. N. Organic Chemistry, Dorling Kindersley
(India) Pvt. Ltd. (Pearson Education).
• Finar, I. L. Organic Chemistry (Volume 1), Dorling Kindersley (India)
Pvt. Ltd. (Pearson Education).
• Finar, I. L. Organic Chemistry (Volume 2: Stereochemistry and the
Chemistry of Natural Products), Dorling Kindersley (India) Pvt. Ltd.
(Pearson Education).
• Eliel, E. L. &Wilen, S. H. Stereochemistry of Organic Compounds;
Wiley: London, 1994.
• Kalsi, P. S. Stereochemistry Conformation and Mechanism; New Age
International, 2005.

SEC
Name of the Department of Microbiology
Department
Name of the Program B.Sc. (Hons. with Research) Biotechnology
Course Code
Course Title CYTOLOGICAL TECHNIQUES
Academic Year I
Semester II
Number of Credits 2
Course Prerequisite

52
Course Synopsis This course provides an in-depth exploration of principles, methodologies
and applications of cytological techniques in biomedical research, clinical
diagnostics, and related fields. Students will learn essential laboratory skills
and theoretical knowledge necessary for sample preparation, microscopy
and interpretation of cytological specimens.

Course Outcomes:
At the end of the course students will be able to:
CO1 Understanding the principles and methodologies of cytology

CO2 Demonstration proficiency in sample preparation techniques for cytological analysis,

including fixation, staining and mounting

CO3 Identifying and classifying different cell types based on morphological characteristics.

CO4 Performing microscopic examination of cells tissues to detect abnormalities or pathologies

CO5 Applying cytological techniques in research, clinical diagnostics and other relevant fields.

Mapping of Course Outcomes (COs) to Program Outcomes (POs) & Program Specific
Outcomes:
COs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 3 - - - - 2 - 3 3 3
CO2 3 - - - - 2 - 3 3 3
CO3 3 - - - - 2 - 3 3 3
CO4 3 - - - - 2 - 3 3 3
CO5 3 - - - - 2 - 3 3 3
Avg 3 - - - - 2 - 3 3 3
1= Weak Correlation 2= Moderate Correlation 3= Strong Correlation

Course Content:
L (Hours/Week) T (Hours/Week) P (Hours/Week) Total Hour/Week
4 4
Unit Content & Competencies
53
 1. Study a representative plant and animal cell by microscopy.
2. Study of the structure of cell organelles through electron micrographs.
3. Cytochemical staining of DNA – Feulgen.
4. Study of polyploidy in Onion root tip by colchicine treatment.
5. Identification and study of cancer cells by photomicrographs.
6. Study of different stages of Mitosis.
7. Study of different stages of Meiosis by permanent slides.

Note: The course plan included as an annexure has the details of each unit
with the number ofhours and mode of delivery and pedagogical approach.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture
Practical 28
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL) / Tutorial
Problem Based Learning (PBL)
Case/Project Based Learning (CBL)
Revision
Others If any:
Total Number of Contact Hours 30

Assessment Methods:

Formative Summative
Multiple Choice Questions (MCQ) Viva-Voce
Viva-voce University Examination
Mid Term Practical Examination Practical Examination & Viva-voce
Quiz
Seminars/ Presentation
Professional Activity

54
 Mapping of Assessment with Cos

Nature of Assessment CO1 CO2 CO3 CO4 CO5

Quiz ✓ ✓ ✓ ✓ ✓
VIVA ✓ ✓ ✓ ✓ ✓
Assignment / Presentation ✓ ✓ ✓ ✓ ✓
Professional Activity ✓ ✓ ✓ ✓
Practical Log Book / Record Book ✓ ✓ ✓ ✓ ✓
Mid Semester Examination 1 ✓ ✓ ✓ ✓ ✓
Mid Semester Examination 2

University Examination ✓ ✓ ✓ ✓ ✓

Feedback Process 6. Student’s Feedback

References: (List of reference books)

• Hardin J, Bertoni G and Kleinsmith LJ. (2010). Becker’s World of the
Cell. 8th edition. Pearson.
• Karp G. (2010) Cell and Molecular Biology: Concepts and
Experiments. 6th edition. John Wiley & Sons. Inc.
• De Robertis, EDP and De Robertis EMF. (2006). Cell and Molecular
Biology. 8th edition. Lipincott Williams and Wilkins, Philadelphia.
• Cooper, G.M. and Hausman, R.E. (2009). The Cell: A Molecular
Approach. 5th Edition. ASM Press & Sunderland, Washington, D.C.;
Sinauer Associates, MA.

SEC
Name of the Department of Microbiology
Department
Name of the Program B.Sc. (Hons. with Research) Biotechnology
Course Code 55
Course Title ORGANIC CHEMISTRY SKILL LAB
Academic Year I
Semester II
Number of Credits 2
Course Prerequisite
Course Synopsis The organic chemistry laboratory course offers hands-on experience in
performing fundamental organic chemistry experiments. Students will gain
practical skills in synthesis, purification and analysis of organic compounds.
Emphasis is placed on safety, proper laboratory techniques, and accurate
record-keeping.

Course Outcomes:
At the end of the course students will be able to:
CO1 Proficiency in basic organic chemistry laboratory techniques such as weighing,
measuring, mixing, heating and cooling
CO2 Competence in purification techniques such as distillation and chromatography

CO3 Understanding the organic reactions and mechanisms through experimental observation
and analysis.

CO4 Skill in experimental design, execution, and troubleshooting in laboratory settings

CO5 Exposure to techniques and instrumentation used in organic chemistry and related field
like biochemistry and pharmaceuticals.
Mapping of Course Outcomes (COs) to Program Outcomes (POs) & Program Specific
Outcomes:
Cos PO1 PO2 PO3 PO4 PO5 PO6 PO7 PSO1 PSO2 PSO3

CO1 2 - - - - 2 - 2 3 3
CO2 2 - - - - 2 - 2 3 3
CO3 2 - - - - 2 - 2 3 3
CO4 2 - - - - 2 - 2 3 3
CO5 2 - - - - 2 - 2 3 3
Avg 2 - - - - 2 - 2 3 3

Course Content:
56
 L (Hours/Week) T (Hours/Week) P (Hours/Week) Total Hour/Week
4 4
Unit Content & Competencies
1. Checking the calibration of the thermometer
2. Purification of organic compounds by crystallization using the following
solvents: a.Water b. Alcohol c. Alcohol-Water
3. Determination of the melting points of unknown organic compounds
(Kjeldahl method and electrically heated melting point apparatus).
4. Effect of impurities on the melting point – mixed melting point of two
unknown organic compounds.
5. Determination of boiling point of liquid compounds. (boiling point lower
than and more than 100 °C by distillation and capillary method).
6. Chromatography
a.Separation of a mixture of two amino acids by ascending and horizontal
paper chromatography
b.Separation of a mixture of two sugars by ascending paper
chromatography c.Separation of a mixture of o-and p-nitrophenol or o-and
p-aminophenol by thin layer chromatography (TLC).
7. Detection of extra elements
8. Organic Preparations
1. (i) Bromination of acetanilide / aniline / phenol
2. (ii) Nitration of nitrobenzene / toluene.

Learning Strategies and Contact Hours

Learning Strategies Contact Hours
Lecture
Practical 28
Seminar/Journal Club
Small group discussion (SGD) 2
Self-directed learning (SDL)/Tutorial
Problem Based Learning (PBL)
Case/Project Based learning (CBL)
Revision
Other if any
Total Number of Contact Hours 30

Assessment Methods:

Formative Summative
Multiple Choice Questions (MCQ) Viva-Voce
Viva-voce
Mid Term Practical Examination University Examination
Quiz
57
Seminars/ Presentation
Professional Activity

Mapping of Assessment with Cos

Nature of Assessment CO1 CO2 CO3 CO4 CO5

Quiz ✓ ✓ ✓ ✓ ✓
VIVA ✓ ✓ ✓ ✓ ✓
Assignment / Presentation ✓ ✓ ✓ ✓ ✓
Professional Activity ✓ ✓ ✓ ✓ ✓
Practical Log Book / Record Book ✓ ✓ ✓ ✓ ✓
Mid Semester Examination 1 ✓ ✓ ✓ ✓ ✓
Mid Semester Examination 2

University Examination ✓ ✓ ✓ ✓ ✓

Feedback Process 6. Student’s Feedback

References: (List of reference books)

• Morrison, R. N. & Boyd, R. N. Organic Chemistry, Dorling Kindersley
(India) Pvt. Ltd. (Pearson Education).
• Finar, I. L. Organic Chemistry (Volume 1), Dorling Kindersley (India)
Pvt. Ltd. (Pearson Education).
• Finar, I. L. Organic Chemistry (Volume 2: Stereochemistry and the
Chemistry of Natural Products), Dorling Kindersley (India) Pvt. Ltd.
(Pearson Education).
• Eliel, E. L. &Wilen, S. H. Stereochemistry of Organic Compounds;
Wiley: London, 1994.
• Kalsi, P. S. Stereochemistry Conformation and Mechanism; New Age
International, 2005.

58