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                                          sequence (5)                                                                                                                                                    Download
                                                   Select Enzymes                     Graphical View                        Enzyme List                         Fragments                          Gel
Graphical View
Enzyme List
                                             Specifications                                                                Parameters                                 Color 
Sequence                                     Gel:     Agarose                                       1%                      Length:      102    mm                          White on black
                                                                                                                                                                            Black on white
                                             Marker: 1 kb DNA Ladder                                                        Time:              minutes
ORF Summary
                                                                                                                            Electical Field:             V/cm
                                             Methylation             Mark affected bands
Flanking Sites
                                                   CpG              Dam              Dcm                                      Ok       Reset
Custom Digest                                      EcoBI            EcoKI
                                                           Marker Unmethylated                                        List: UnMethylated Lane
Results For:        Edit                   Size
                                            (bp)
      Enzymes:                                                                                                          #           Ends                             Coordinates         Length         Affected by
       - Supplier: Any                    10000                                                                                                                                          (bp)           Methylation
       - Type: Type I, II, III, Homing,
                                          5000                                                                          1           EcoRI - EcoRI                    44973-21226        24756           CpG
      Nicking
      ORFs:                                                                                                             2           EcoRI - EcoRI                    31748-39168        7421
       - Min: 100 aa
                                                                                                                        3           EcoRI - EcoRI                    39169-44972        5804
       - Searched                         1000
                                                                                                                        4           EcoRI - EcoRI                    26105-31747        5643
Sequence Information: 
                                                                                                                        5           EcoRI - EcoRI                    21227-26104        4878            CpG
48502 bp, Circular                         500
GC = 50%, AT = 50%
Analysis Warnings: 
      Too many sites
   View details
                                           100
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