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NEBcutter 3.0

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jens leuckx
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0% found this document useful (0 votes)
77 views1 page

NEBcutter 3.0

Uploaded by

jens leuckx
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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v3.0.

19 WELCOME GUEST, SIGN IN OR SIGN UP FEEDBACK HELP

Create New Project


sequence (5) Download

Select Enzymes Graphical View Enzyme List Fragments Gel


Graphical View

Enzyme List
Specifications  Parameters  Color 

Sequence Gel: Agarose 1% Length: 102 mm White on black


Black on white
Marker: 1 kb DNA Ladder Time: minutes
ORF Summary
Electical Field: V/cm
Methylation  Mark affected bands
Flanking Sites
CpG Dam Dcm Ok Reset

Custom Digest EcoBI EcoKI

Marker Unmethylated List: UnMethylated Lane


Results For:  Edit Size
(bp)
Enzymes: # Ends Coordinates Length Affected by
- Supplier: Any 10000 (bp) Methylation
- Type: Type I, II, III, Homing,
5000 1 EcoRI - EcoRI 44973-21226 24756 CpG
Nicking
ORFs: 2 EcoRI - EcoRI 31748-39168 7421
- Min: 100 aa
3 EcoRI - EcoRI 39169-44972 5804
- Searched 1000
4 EcoRI - EcoRI 26105-31747 5643
Sequence Information: 
5 EcoRI - EcoRI 21227-26104 4878 CpG
48502 bp, Circular 500
GC = 50%, AT = 50%

Analysis Warnings: 
Too many sites

View details
100

Looking for NEBcutter v2? It's still avaliable at https://nc2.neb.com, but is no longer being updated.

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