HEXON QUALITY ASSURANCE
LABORATORY
Hexon Quality Assurance Laboratory
Measurement Uncertainty Form
Document No: HEXON/QAL/7.6/F-16
Test Title Validation of Method of Imidacloprid determinations
Test Method CIPAC MODIFIED IMIDACLOPRID METHOD
Method Reference 8.1. CIPAC Vol K page # 70
Test Date 27-03-2019,24-04-2019
Equipment HPLC Method
S # Observations repeatability Reproducibility SD (R)
1 Reading 1 25.03 25.04 -
2 Reading 2 25.06 25.06 -
3 Reading 3 25.04 25.02
4 Reading 4 25.07 24.99
5 Reading 5 25.01 25.04 -
4 Average 25.042 25.030 0.008
5 SD (r) 0.024 0.026 -
Uncertainty of Repeatability i.e. SD (r) 0.026 units
Uncertainty of Reproducibility i.e. SD (R) 0.008 units
Average from MAX SD (r) 25.030 units
Average from SD (R) 25.036 units
UNCERTAINTY MEASUREMENT (Budget)
S# Sources Uncertainty Type A/B Divisor Unc Cont
1 Repeatability 0.024 A 1 0.024
2 Reproducibility 0.026 A 1 0.026
3 Calibration Certificate 0.000 B 2 0.000
4 CRM 0.005 B 1.73 0.003
5 1/2 Resolution 0.0001 B 2 0.00005
6 Dilution (weight/Volume) 0.004 A 1.73 0.00231213873
7
Combined Uncertainty (Uc) 0.036 @ 68 % CL
Confidence Level (K) 2 = 95% CL
Expanded Uncertainty (Ue) 0.071 @ 95% CL
0.100
Result Summary
Expanded Uncertainity 0.100
Upper Result Limit (+) 25.136 units
Average 25.036 units
Lower Result Limit (-) 24.936 units
no need
Imidacloprid 25.036 ± 0.1 this is reported results
Prepared by Lab Assistant Approved by Quality Assurance Manager
Formulas Used in Calculation of Measurement of Uncertainty
S_x = √((∑_( i=1)^( n)▒ 〖
Standard Deviation (x_i-x ̅ ) 〗 ^2 )/(n-1))
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x ̅ = The mean of x_i
n = The number of data points
x_i = Each of the values of the data
CU = √(∑▒ 〖〖 U 〗 _r^2+ 〗
Combined Uncertainty
U_R^2+U_e^2+U_m^2 )
U_r = Uncertainty of Repeatability
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U_R = Uncertainty of
U_e =
Reproducibility
U_m =
Uncertainty of Equipment
Uncertainty of Method
