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Dercaabe_pinciple Prefamo Hon_of stain.
ufed in H/E Staining. wnteolbout
Lomplee proceslre of H/E Staining
claning& mounkng of tissul
wha ane tissuR fixaHve & wite HSE
staining poceditme in detail
Enuneonle. five stain ued in histoahalogy
Section Wite in dotailabout PnAciple
pxdeeduE of Ho om0dozylino eaxin Staining
Discur indetail PinupleMehod step of
hematoxyling 2 easin staining
MLnlion Namour stain used ia histopahaalogy
oike in details_ahout gminciple 4 pcedtne
ofhaemotozylineoria_Saining: (AIl amg tor
mank
amo of Hha stain using in histopa Hhalog
LabratDy
O Hemadorylin & Eosin stain (H&E tain)
Rekiuin 5tan-
Pem Aid schiffstain (PAs)
)Romonous Ky5tains
Paponicoloous Stain \eAP)
Gimsa stain:
Piatiple:
Hemadoxylin eoSinCH*E Ome Hhe pnnia
Sstain ued foa Hhe demonstmlion of
ucleus he Cytoplasmic inclusioNS
Alum acts a moxoond -hematomylin_
Containing Alum Stains he nucLeur
light blúL. which tumi zed in the
Presence pf Acid
The ceU dilteentiaHo0 5 adhlened by_
treating the. tissul with Acis soluuhon
The DunLis Staining pestommes by
using eosin soluHon hich inmpark
Pink Lnlous to_Lytoplasm
Requurement
OTCoplingor
DaDepina4 bottles (soml)
-Cove rlip
DisSecting needle fomceps, Scalpels efe
03dindmy iilka_Lapes
O TDX01 Coaada baSamMountaMt
idR tooshing tsay
Reaent
Hai honaatonylin stain
Dissolve 1.09 of hematoxylin in
albout 10 m gs ethanoin amONtOI
im a Pertle
Dissolne 20 0t ammonium_Qluumin
200m ofhak odistil|l-ed wales
Tmixk soluton a ' b'uhile hof
bmng 9Likly to boil with
Lonstant
bing
slimn9
Aldd 0S90ft mestumc oxide R-ema
the flome (o 0s apidly os_porsibl-e
o itlh use of Unain9 tap uo0
Cillex dstore nanAmber olomed
bottle
i s stainis stab.le ad TDom emp
2°c to 30°e)tox Senisal month
2 fosin 5olurion_(forin Stain)
Dissolve. 1.0 o t yellou earin_0
Obout 80m of sisHlled u a t .
320 m gr'l eHaanal
Add D.4 mof glaua acekic mom acLo
lemp
T i s SoluHoni5 Stable at
(25 ° t o _30°CO & for sensml moathJ
oS].hyahmchlonc Aio
4Dilute Ammonja waler.
stainin4 PoDcedlure
DePonaffinize. He Section
lome he5lide dn a buNNON
hen_p14Le 1kin Xylene for 3 tou MN
Refeat ylene treatmlnt with agitako
Take heSectionto ualerlhydnokon)
Hydnae he Setionby pa4sing it
Hamugh deseasn9 Conc op alcwhol
babht a e s
The alcohol Solufion usesgno-
place the section fox30 to Co sepe_
Lo ach_of HheAe afcohol solukions
ash in tap OAl sinse in
disHlled wakx
Donin he SeHon well b-efone
Staining
staning
O5tain heSeio with Hematoxuylán
Sal f 3-SmA:
wosh in unAing taP cwaka
uikly dip the slide inout of s
0. hydwdhloc acid
Qkly iare Hac slide intap waters
fox 30 to 6o sec.
DipHhe slide Senenl time i o
luR OMmoua watex_
G)uO0h with tap woLex & mnse with
9 . Alcohol
6 Agitaue.tha slide in £osin sal tor
10 to 60 sec Dno Hhe
Staininq So
Dehyarakion :
lace he slido ia Zo. Alcoha
OPloce
TOY 3o to 60 5ec:
Place in 4r AlLoho fos 30 to 601C
-place in Aksolu_AlLahol
5 cleamng toee ia two timer
place Hhe slidl
to 6 o 5eL each_
i A Xylene toS 0
6MounHng mount
Dxaun he eACesS Xylene
0 T Conada balcam with
o n DPX_
CoveKZlip
*ResulhNuclei Blue Co lau
Cel
nytho(yte Red Coloux
Muncle Conneine tiss ul ytoglasm
Vomying shadksot pink._
ualiy Lanto
Multiple slides can be lut : kepB
Aside & ued as Contml using
fallocoing tissuR=Cenix lymPh pade
Add Note o 5peiol stain_in
HistopaHhology
special staiaing techniLL_meanr any
Stainin technique othea Haan H4E
Speial stains RVea taickurer lik_
kiuulin fib eloMtin £tC
There on anillan fechniu
LSedto
Suppot the diognoris of aSpecitic
rliaical Londition
Spetiol taining technißLtoCan be
USed fo idenHfitatiN_of oxganims
toeLcgni2ePipmeats
kidney
to_Sugpor
line biopsies, gasta inkstina.
oioses ymph noder biopsies_etc
in Hhedignofi o
Vomouf tum0S
Name of he sPecialstajns
ORetiuulin stain
PAs stain Croenod Acid schiff stain)
Oiemsa stoUn
_PaPanu ColaouSStain PAP)
Retiulin stain
mAuple
1n he (eaneekiu tisSuxeticline
appear Or tibyllaomyextracellulan tsam
he fibnlf ot eilLAStain black aff
iMRgnakioni t a silers
Thir kype of stainiA9 15 ufed to
identiy tumor_OnUNcONkai tain_onmqLn
PAs_ stain CPeiod Aciol schiff stain
PriAciple
tssuStruchre tike isihtdrt,
Piudic acid-schiff stain_is bareal
0n PemodicAcid xeaLts with
Aldkhyde qmuP of th Cabohyohsates
AflexLoy oT0cHon with thas
Shuf' ea9ent Padducer red or
punple-TedColour._
G CimsaShain
Pniple
tStatn is GaemSo Stain is_O
oifferential taln-
That pmeanr it helps to differeatiako
both oacidlic_i bapc_Componentof
Cells.
This stain_Contan azUne,Mthy leng
blue forin dye
AcidiC dy er-_Azune EDsin
Baric dyes: methylene blue.
pAp stain Papanicolaous 4tain)
nale-
The
is tob_ easypngople af AP stain
distinguish _betueen
bosmphilic & Acidoßhilic cel
ehtoin adetaiLed chmnaHn Componcat-
