KANCHAN SHEKHAWAT                                       XII SCIENCE (2024 – 20
PM SHREE JAWAHAR NAVODAYA VIDYALAYA, KAJRA
                      BIOLOGY PROJECT FILE
                         SESSION :- 2024-25
    PROJECT –
    REPORTED BY – KANCHAN SHEKHAWAT
    CLASS – 12th SCIENCE        ,       ROLL NO. –
                    REPORTED TO – MR.SUNIL YADAV
                         ( P.G.T. BIOLOGY )
SIGN. OF EXTERNAL EXAMINER                    SIGN. OF PRINCIPAL
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KANCHAN SHEKHAWAT                                                                          XII SCIENCE (2024 – 20
                                      CERTIFICATE
                                                                                          Type your text
  This is to certify that KANCHAN SHEKHAWAT of class XIIScience roll no. has satisfactorily completed her
  Biology investigatory project on Microbes in human welfare under the guidance of Mr. SUNIL YADAV
  as prescribed by the course during the academic year 2024 – 2025.
                                                                      INTERNAL EXAMINER’S SIGN.
 EXTERNAL EXAMINER’S SIGN.                                                         PRINCIPAL
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KANCHAN SHEKHAWAT                                                                          XII SCIENCE (2024 – 202
                               ACKNOWLEDGEMENT
 I would like to sincerely and profusely thank my biology teacher Mr. (PGT BIOLOGY)
 and our lab attendant for their able guidance and support in completing my project.
 I would like to extend my gratitude to the principal MR. SUNIL YADAV for providing me with all the
 Facility that was required.
    SIGN. OF CANDIDATE
                                              CONTENTS
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KANCHAN SHEKHAWAT                                                                     XII SCIENCE (2024 – 20
 1. Introduction …………………………………………………………………………………… 5
           Microbiology
           What are Microbes ?
           Kinds of Microbes
 2. Microbes in Households ………………………………………………………………………… 8
 3. Microbes as Biocontrol agents …………………………………………………………………... 10
 4. Microbes as Fertilizers …………………………………………………………………………….. 12
 5. Bibliography …………………………………………………………………………………………. 14
                                  INTRODUCTION
       MICROBIOLOGY :
 The branch of science which deals with the study of microorganisms It further numerous sub
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KANCHAN SHEKHAWAT                                                                          XII SCIENCE (2024 – 20
 disciplines including virology, bacteriology , parasitology, mycology etc.
    ➢ WHAT ARE MICROBES ?
 Microbes are tiny living things that are found all around us . They may or may not be
 visible to the naked eye. Microorganisms are very diverse; they include bacteria, fungi, algae,
 and protozoa; microscopic plants (green algae); and animals such as rotifers and planarians.
 Microorganisms live in all parts of the biosphere even where no other life form can exist, such
 as in deep water, including soil, hot springs, on the ocean floor, high in the atmosphere and
 deep inside rocks within the Earth's crust.
 Microorganisms are critical to nutrient recycling in ecosystems as they act as decomposers. As
 some microorganisms can fix nitrogen, they are a vital part of the nitrogen cycle, airborne
 microbes may play a role in precipitation and weather. Microbes are also exploited by people
 in biotechnology, both in traditional food and beverage preparation, and in modern
 technologies based on genetic engineering.
 Pathogenic microbes are harmful, since they invade and grow within organisms , causing
 diseases that kill humans, plants & animals.
    ➢     KINDS OF MICROBES
 There are different types of microbe present on our earth, they are :-
 1. Algae
        Algae are a diverse group of eukaryotic, non-vascular thallophytes, autotrophic,
        Chlorophyl containing organisms capable of producing oxygen through
        photosynthesis.
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KANCHAN SHEKHAWAT                                                                           XII SCIENCE (2024 – 20
 2. Bacteria
        Bacteria are very tiny single-cell microbes that can live in all environmental
        conditions.
 3. Protozoa
        Protozoa are microscopic unicellular eukaryotes, either free-living or parasitic.
 4. Virus
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KANCHAN SHEKHAWAT                                                                          XII SCIENCE (2024 – 20
        A virus is a submicroscopic infectious microbe that replicates only inside the living
        cell of an organism.
  5. Fungi
             Fungi are eukaryotic, multicellular organisms that include Yeasts , moulds, etc
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KANCHAN SHEKHAWAT                                                                                    XII SCIENCE (2024 – 20
                     MICROBES IN HOUSEHOLDS
 Microbes and their products are used everyday in the making of cheese, curd, dough, bread, vinegar
 and other foods in everyday life. Lactic acid bacteria (LAB), also known as Lactobacillus acidophilus,
 Lactobacillus lactis, and Streptococcus lactis, are probiotic bacteria that inhibit the growth of harmful
 bacteria in the stomach and other regions of the digestive tract .
  Curd: Microorganisms such as Lactobacillus and others commonly called Lactic Acid Bacteria (LAB)
 grow in milk and convert it into curd. During growth, the Lactic acid bacteria produce acids that
 coagulate and partially digest the milk proteins called casein.
 Paneer: Paneer (cottage cheese) is a fresh cheese common in South Asia ,especially in India. It is
 made by curdling milk with lemon juice, vinegar and other edible acids. Large holes in Swiss cheese is
 due to the production of a large amount of carbon-di-oxide by the bacterium Propionibacterium
 shermanii.
  Idli and Dosa: The dough which is used for making dosa and idli is the fermented preparation of rice
  and black gram (Urad dal).
 1. The dough is allowed to ferment overnight for 10–1210–12 hours.
 2. The lactic acid bacteria from Leuconostoc and Streptococcus species bring about fermentation.
 Several other genera, like Bacillus,Candida, and Saccharomyces, are also used in this process.
 3. During fermentation, CO2 is evolved, which causes doughing of the raw material. The bubbles
 of CO2 trapped in gluten makes the idli puffy.
 Yoghurt: Yoghurt provides protein and calcium, and it may enhance healthy gut bacteria.
 1. Yoghurt is produced by curdling milk with the help of Streptococcus thermophilus and Lactobacillus
 bulgaricus, Streptococcus lactis, etc.
 2. Initially, milk is heated to 80 –90 C80 –90 C for half an hour. It is cooled to 40 –43 C and mixed with
 an inoculum containing the Streptococcus thermophilus bacteria.
 3. After four hours, yoghurt becomes ready. It has an original flavour of lactic acid and acetaldehyde.
 4. Since it is already acidic (pH3.7–4.3),(pH3.7–4.3), yoghurt is often sweetened and flavoured with
   fruits.
 Bread: Bread is made from wheat flour.
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KANCHAN SHEKHAWAT                                                                           XII SCIENCE (2024 – 20
  1. The dough that is used for making bread is fermented by using themicrobe Saccharomyces
     cerevisiae which is commonly called Baker’s Yeast .
  Cheese: Different varieties of cheese are known for their characteristic texture, flavour and taste. The
  specificity comes from the microbe that is used in it. For example, the large holes in ‘Swiss cheese’ are
  due to the production of a large amount of CO2CO2 by a bacterium named Propionibacterium
  shermanii. In contrast, the ‘Roquefort cheese’ are ripened by growing a specific fungus on them, which
  gives them a    specific flavour.
  Pickles: Pickles prepared by lactic acid bacteria (LAB) fermentation have a unique flavour and beneficial
  health effects. These LABs are slightly different as they can comfortably tolerate higher concentrations
  of salt and sugar. Lactobacillus plantarum, L. brevis, Leuconostoc mesenteroids and pediococcus
  cervisiae are used in making pickles.
              MICROBES AS BIOCONTROL AGENTS
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KANCHAN SHEKHAWAT                                                                                        XII SCIENCE (2024 – 20
  ➢WHAT IS BIOCONTROL?
  The natural method of eliminating and controlling the insects, pests and other disease-causing agents
  using their natural, biological enemies is called biocontrol or biological control. The agents which are
  employed for this are called biocontrol agents. Microbes are one of them. Biocontrol works on the
  principle of predation. It is much reliable and healthier than killing insects and pest using insecticides
  and pesticides.
  Thus, this prevents soil pollution and health issues related to insecticide poisoning, etc.
  ➢BIOCONTROL AGENTS
  Biocontrol agents are an integral part of organic farming. In organic farming, farmers believe in
  mutualism. In other words, organic farmers keep a balance of useful and harmful agents within the
  system. The chemicals used for eradicating pest and parasite might not be always successful and also
  harm useful agents too. Instead, farmers used biocontrol agents which predate the insects and pests
  that cause diseases to crops.
  ➢MICROBIAL BIOCONTROL AGENTS
  Ladybird and dragonflies are two common insects which are employed to eradicate aphids and
  mosquitoes respectively. Other than insects, microbes are also used as biocontrol agents. These
  microbes include bacteria, fungi, viruses, and protozoans. Microbes act as biocontrol agents in three
  ways, either they cause diseases in the pests or compete with them or kill them.
  Biotechnology has extended widely and has developed many biocontrol agents. For example, Bacillus
  thuringiensis which is often referred to as Bt is a microbial biocontrol agent. Spraying the solution of Bt
  made of spores on plants will kill the butterfly caterpillars. The spores that are ingested release toxins in
  the guts of the larvae and kill them. Biotechnology has developed disease-resistant and pest-resistant
  plants by injecting the toxic genes of B. thuringiensis into plants. For e.g. Bt cotton. Fungus-like
  Trichoderma and baculoviruses of genus Nucleopolyhedrovirus are some other microbial biocontrol
  agents. These agents are specific in nature; this along with the cost limits their use.
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KANCHAN SHEKHAWAT                             XII SCIENCE (2024 – 20
                    MICROBES AS FERTILIZERS
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KANCHAN SHEKHAWAT                                                                                      XII SCIENCE (2024 – 202
  The Biofertilizer is a substance containing Microorganisms that help with Soil Fertility, and this helps in
  making the plant grow in a healthy way. This also helps in increasing the stream of vital nutrients in
  trees and plants. The Biofertilizers impart living organisms like mycorrhizal fungi, blue-green algae plus
  other types of bacterial Biofertilizers. The role of the bacteria as a form of Biofertilizers helps with the
  restoring of Soil’s organic matter, and the regulation is done by natural.
  There are various types of Microorganisms that act as the biofertilizers.
  Each of the types comes with its own exclusive capability and function.
  There are mainly 6 types of the Microbes that are used as the
  Biofertilizers. These Microbes are Rhizobia, Azotobacter , Azospirillum, Blue-green algae, Azolla, and
  Phosphate-solubilizing Microorganisms
  ➢ IMPORTANCE OF BIOFERTILIZERS
          The Biofertilizer Microbes are utilized for fixing the nutrients within the Soil, especially related
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KANCHAN SHEKHAWAT                                                                                   XII SCIENCE (2024 – 20
          to the Insufficient Nitrogen.
         The Biofertilizer is the living organism and it helps in converting the composite organic material
          into the simple compounds. This makes it easier for the plants or trees to take them.
        The usage of Microorganisms as fertilizers helps in enhancing the growth of plants enormously
         and it also gives the richness back to the Soil.
        Instead of controlling the growth, the Microorganisms help in advancing the process of growth
         within plants especially related to the growth in new seed.
 The Microorganisms as the Biofertilizers are incredibly cost-effective in comparison to synthetic
 fertilizers. You also have the option of growing it in your farm and then use it for the healthy growth of
 the crops. The micro-organisms also tend to provide good quality nourishment for the plants in
 comparison to the chemical fertilizers.
                                           BIBLOGRAPHY
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KANCHAN SHEKHAWAT                          XII SCIENCE (2024 – 20
  NCERT class 12 book of biology
  MTP NCERT FINGERTIPS BIOLOGY book
        www.google.com
        https://en.wikipedia.org
        https://www.slideshare.com
        https://scribd.com
        www.pinterest.in
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