Curriculum- Vitae

Shalini Aggarwal
HouseShalini
No. A1 704, tdi
Aggarwal
House No. A1 704, tdi city
Kundli
Kundli
Sonipat -131028 e-mail: shalini.25dec@gmail.com
Sonipat -131028
Haryana, India Tel: +911732220009
+918295597999
Haryana, India
Objectives
Looking forward for working in the challenging field with a motivated team at a position
that would utilize my skills and abilities and offers professional growth while being
resourceful, innovative and flexible.

Profile
Research experience in molecular biology, animal cell culture at National Institute of
Immunology (NII), New Delhi.
M.Sc.in Biotechnology from Thapar Institute of Engineering & Technology (Thapar
University), Patiala.
Worked as lecturer in biotechnology at Mukand Lal National College (Kurukshetra
University), Yamuna Nagar (July2008-March2011).
Working as lecturer in biotechnology at S.D.College (PG) (Kurukshetra University), Panipat
(July2011- April2013).

Research Experience:

December 18th 2006 - December 18th 2007

Ø Worked on "Halogenase Involved in Chlorination of Dif-1 Molecule in

Dictyostelium". I was associated with another project which is related to

"Evolutionary Studies of the Gene Cluster in Various Species of

Mycobacteria" wherein I particularly worked on M. paratuberculosis. Advisor:

Dr. Rajesh S. Gokhale (Director IGBI and Senior Scientist at NII New Delhi),

National Institute of Immunology, New Delhi.

January 11th 2006 to June 30th 2006

 Ø Six months project work entitled "Value Added Products from W aste Stream
from Industry Effluent''. Advisor: Dr. Abhijit Ganguli, Thapar Institute of
Engineering and Technology, Patiala, Punjab.

June 1st 2005 to July 15th 2005

Ø A 45 days summer training project work entitled "Studies on Glutamate

Dehydrogenase from Strain PPD (Pseudomonas sp.) Grown On Phthalate
Isomers". Advisor: Dr. P. S. Phale, School of Biosciences and Bioengineering,
Indian Institute of Technology-Bombay, Mumbai.

Ø Summer training at Daurala Sugar Works, Daurala, Meerut (U.P.), a division of

shriram group of industries in 2003.
Ø Summer training at MOREPEN Laboratories, Solan (H.P.) in 2003.
Ø 28 Days summer training at National Dairy Research Institute (NDRI), Karnal
- Under guidance of Dr. V. K. Batish, Head Biotechnology division in 2002.

Academic Profile:

Degree Board / University Year of Subjects

passing
M.Sc. Thapar institute of 2006 Cell & Molecular Biology, Bio Techniques, Microbiology,
Biotechnology engineering and technology, Bioengineering, Biochemistry, Genetic Engineering, Industrial
Patiala. Microbiology, Waste Management, Bioinformatics.

B.Sc. Kurukshetra University, 2004 Industrial Microbiology, Botany And Chemistry

Biology Kurukshetra

Intermediate Sacred Heart Convent 2001 English, Physics, Chemistry& Biology

School, Jagadari.
C.B.S.E.
Matriculation (class Sacred Heart Convent 1999 English, Mathematics, Science, Social Science & Hindi
th
10 ) School, Jagadari.
C.B.S.E.

Practical exposures:

· Molecular Biology Techniques

· PCR based cloning
· DNA isolation
· Electrophoresis (Agarose, Native- PAGE and SDS-PAGE)
· Western Blotting
· Protein isolation and estimations, Purification.

· Immunological Techniques
· Radio immunoassay.
· Immunohistochemistry (IHC).
· ELISA
 · FACS
· ELISPOT

· Biochemical and Biophysical methods

· Various types of chromatography (Affinity, TLC, HPLC, FPLC)
· Mass and UV spectroscopy
· MALDI TOF-TOF

· Microbiology
· Microscopy
· Bacterial Culture Handling
· Filtration Technique
· Staining techniques
· Batch scale production

· Animal Cell Culture and Tissue Culture

· Maintenance of cell lines.
· Establishing new cell lines.

Computer Knowledge

Basic computer applications in general and biological sciences such as MS Office, Windows,
accessing different online tools for accessing genomic databases and application based software
for protein profiling of sequenced genomes.
Did certification in a Programme organized by Infosys, learned the basic fundamentals of
programming and SQL.2006.

Conferences Attended:

Attended International Colloquium on Biotechnology On 27-28 November ,2009.

Attended state level seminar, Relevance of Basic Sciences in Higher Education in 2009.

Shalini Aggarwal, Moushumi Ghosh, Abhijit Ganguli And Ankit Singhal

Microbial conversion of Starch containing snack food industry wastes to Lactic Acid.
Department of Biotechnology and Environmental Sciences,
Thapar University, Patiala - 147004. Punjab National Congress, Patiala, 7 - 9 Feb 2008.
"Bioconvergence", an international conference of biotechnologists being held at Thapar institute of
engineering and technology, Patiala in 2005.
Attended national workshop on Entrepreneurial Development in Food Processing organized by
STEP, Patiala, in 2006.
Attended Second Teacher's Reorientation in Biological Sciences in 2004.

Hobbies:
· Traveling, Reading.
Personal info:
Date of Birth : 25th December 1983
Father's name : Mr. Pardeep Kumar Aggarwal
Mother's name : Mrs. Usha Aggarwal
Nationality : Indian
Languages known : Hindi, English.

Referees:
Dr. Rajesh S. Gokhale (Senior Scientist),
rsg@nii.res.in
Phone No. +91-9312323767 ,
National Institute of Immunology,
Aruna Asaf Ali Marg,
New Delhi- 110067. .

Dr. Abhijit Ganguli (Asstt. Professor, Food Biotechnology)

aganguli@thapar.edu
Dept. of Biotechnology & Environmental Sciences
Thapar University, Thapar Technology Campus
Patiala 147004, India
Tel:+91-175-2393387(O),+91-175-2393478(R)
Mobile: 09814948811

Prof. Anil Kumar (Professor)

anilkumar@iitb.ac.in
Chemistry Department
IIT Bombay
Mumbai, India 400076.
Tel: +91-2225767153
Fax: +91-2225767152
www.chem.iitb.ac.in/~akumar

Dr. Niranjan Das (Asstt. Professor & H.O.D.)

ndas@thapar.edu
Dept. of Biotechnology & Environmental Sciences
Thapar University, Thapar Technology Campus
Patiala 147004, India
Tel:+91-175-23933127(O),+91-175-2393043

Dr. Rajiv Kalsi ( H.O.D)

rajivkalsi@yahoo.com
Department of Zoology and Biotechnology
M.L.N. College
Yamuna nagar-135001, India.
Tel:+91-1732-220960(Office)
Fax:+91-1732-225560(Tel/Fax)
Tel: ++91-9896114579 (Mobile)
RESEARCH EXPERIENCE

I. Looking For The Probable Halogenase Involved In Halogenation Of Signal

Molecule.

From July 2007 onwards I worked on "Halogenase Involved in Chlorination of Dif-1

Molecule in Dictyostelium". Advisor: Dr. Rajesh S. Gokhale from National Institute
of Immunology (NII) , JNU Complex, New Delhi-110067.

Brief Project methodology:

· Bioinformatics analysis to find the probable genes in dictyostelium genome on the
basis of their homology to already characterized halogenase by other groups.
· Cloning of these genes in different expression vectors.
· Check the expression of the protein and to get the protein in soluble fraction.
· To purify the protein of the contaminating proteins using FPLC.
· To put up assays with probable substrate and to check the product formation on TLC
and HPLC to confirm.
· Analysis of the mass of the sample with the help of Mass spectrometry (LC-MS) and
MALDI-TOF-TOF.
· Analysis of the results.

II. Looking For A Novel Drug Target Against Mycobacterium tuberculosis Aiming
At Vaccine Development

I was associated with another project which is related to "Evolutionary Studies of the
Gene Cluster in Various Species of Mycobacteria".

Brief project methodology:

· Retrieving the gene sequences from respective database and PCR amplification using
genomic DNA
· Cloning and expression of the PKS genes present in the cluster of
M. paratuberculosis.
· Protein expression studies and purification of the protein.
· Radioactive assay with probable substrate and identification of the product by using
TLC, HPLC and finally mass-spec analysis.
· Analysis of results.

M. Sc. Thesis project

From this project, we have got some interesting findings which are going to publish
soon. One has already been communicated in Journal of Food Biotechnology.

Con gress comm un ication s

Ø Presentati ons
§ Shalini Aggarwal, Moushumi Ghosh, Abhijit Ganguli And Ankit Singhal
 Microbial conversion of Starch containing snack food industry wastes to Lactic Acid.
Department of Biotechnology and Environmental Sciences,
Thapar University, Patiala . Punjab National Congress, Patiala, 7 - 9 Feb 2008.

§ Moushumi Ghosh, Shalini Aggarwal And Abhijit Ganguli

Biotechnological productions of Lactic Acid from potato processing waste water by
Lactobacillus plantarum isolated from Fermented Green Chutney's
International Conference on Biotechnological Engineering ICBioE'07,
Kaula Lampur, Malaysia, 8 - 10 May 2007.

From Jan 11th 2006 to June 30th 2006 for partial fulfillment of the Masters program in
Biotechnology. Six months project work entitled "Value Added Products from W aste
Stream of Industry Effluent''. Advisor: Dr. Abhijit Ganguli, Thapar Institute of
Engineering and Technology, Patiala, Punjab.

Lactic acid bacteria possessing amylases can utilize starch fermentatively for lactic acid
production. Amylolytic lactic acid bacteria (ALAB) account for a substantial portion in
different types of foods and are widespread among the non-dairy food environments and
different geographical areas. Some lactobacillus species have been reported to be able to
reduce the hydroxyl group of the polysaccharides into aldehyde group and finally the
other group of bacteria from lactobacillus genera to convert this aldehyde group into an
acid moiety. For these biochemical conversions usually we would require more then one
microbe and also require setting up a multiple fermenter to carry out the two steps
separately which is quite cost intensive. So the idea was to find an organism which could
carry out both the steps of this conversion of starch from waste effluent into lactic acid.

Brief project methodology:

· Screening of the Lactobacillus sp. bacteria which could utilize starch as energy
source
· Starch degradation profile on nutrient medium
· Growth optimization in different media
· Quantification of the amylase activity by lactobacillus plantarum in nutrient
medium
· Temperature and pH optimization for lactic acid production by Lactobacillus
plantarum
· Starch degradation profile in nutrient medium using a dialysis sac
· Morphological & biochemical characterization of the strain

Summer Training

From June 1st 2005 to July 15th 2005 as a 45 days summer training project work
entitled "Studies On Glutamate Dehydrogenase from Strain PPD
(Pseudomonas sp.) Grown On Phthalate Isomers". Advisor: Dr. P. S. Phale,
School of Biosciences and Bioengineering, Indian Institute of Technology-
Bombay, Mumbai. The work was published as Biodegradation of phthalate
isomers by Pseudomonas aeruginosa PP4, Pseudomonas sp. PPD and
 Acinetobacter lwoffii ISP4 in Applied Microbiology and Biotechnology. 2006
Oct; 72(6):1263-9. Epub 2006 Apr 11.

This lab is basically a metabolic engineering lab and the work is mainly focused on
studying biodegradation of phthalates. Our aim was to look at the enzymatic activity of
GDH (Glutamate Dehydrogenase) produced by a strain of Pseudomonas sp. which
actually is responsible for cleavage of closed ring structures and to convert them into
aliphatic compounds so as to enhance the biodegradation of these recalcitrant compounds.
This study included growing this strain on different phthalate isomers and study their
growth profiles and also to see the effect of addition of glutamate on their profiles in
minimal salt media and then looking for the enzyme activity using spectrophotometer and
performing activity staining (a substrate-enzyme based activity reaction on native PAGE).