HEMATOLOGY
MAJOR PRACTICALS QUESTIONS
1. Name the WBC diluting fluid, Give its composition and function
2. Name the other fluid used as WBC diluting fluid.
3. What is the dilution factor for WBC count and why
4. Give the normal WBC count and give the Difference between Leucocytosis and
Leukemia
5. Give examples for Pathological and Physiological increase and decrease of WBC
count
6. List the Functions of each Leucocyte
7. Name the staining agent used for Differential count, Give its composition and
functions
8. Name the oil used for Differential count and why
9. What is the duration of fixation time and staining time in the Differential count slide
preparation
10. Give the normal distribution percentage of WBCs in Differential count
11. List the granules present in the eosinophil
12. What are the features of an ideal smear
13. Give examples for Pathological increase of
Neutrophil/Lymphocyte/Monocyte/Eosinophil
14. Give examples for Pathological decrease of
Neutrophil/Lymphocyte/Monocyte/Eosinophil
15. List the identifying features for granulocytes and agranulocytes
MINOR PRACTICALS QUESTIONS
1. Name the method used for Haemoglobin estimation and mention the principle of the
method
2. What are the other methods used for Haemoglobin estimation?
3. Give the Normal Haemoglobin value for males , females, new born
4. What is the oxygen carrying capacity of 1gm Haemoglobin
5. List the normal, embryonic and abnormal haemoglobins with their chain structure
6. Haemoglobin Appears in which stage of erythropoiesis?
7. Name the method used Erythrocyte sedimentation rate and mention the anticoagulant used
8. Give the normal value for ESR in males and females
9. Give examples for increase and decrease of ESR
10. List the factors affecting ESR
11. Give examples for invivo and invitro anticoagulants
12. Name the method used for Packed cell volume(Haematocrit)
13. What are the three layers of haematocrit
14. Give the normal PCV value in males and females
15. Give examples for increase and decrease of PCV
16. Why haematocrit of venous blood more than arterial blood
17. State Landsteiner’s law
18. List the different Blood group systems
19. Which blood group is the universal donor, universal recipient?
20. Explain Major and Minor cross matching
21. Give the difference between ABO incompatibility and Rh incompatibility
22. What are the hazards of mismatched Blood transfusion
23. Give the significance of Blood Grouping
24. Erythroblastosis Foetalis
25. Calculation of RBC indices (MCV, MCH, MCHC, CI) using the values given (Hb, RBC,
PCV)