Introduction to Histology
1. Definition and Importance
Histology is the study of tissues at the microscopic level.
Essential for understanding the structure-function relationship in organs and systems.
2. Types of Tissues
Epithelial Tissue:
o Covers body surfaces and lines cavities.
o Types:
Simple Squamous: Thin, facilitates diffusion (e.g., lungs).
Stratified Squamous: Protection (e.g., skin, esophagus).
Cuboidal: Secretion (e.g., glands, kidney tubules).
Columnar: Absorption (e.g., intestine).
Connective Tissue:
o Provides support and structure.
o Types:
Loose Connective Tissue: Supports organs.
Dense Connective Tissue: Found in tendons and ligaments.
Adipose Tissue: Stores fat.
Cartilage: Provides flexible support.
Bone: Rigid support and mineral storage.
Blood: Transport of oxygen and nutrients.
Muscle Tissue:
o Responsible for movement.
o Types:
Skeletal Muscle: Voluntary movement.
Cardiac Muscle: Heart contractions.
Smooth Muscle: Involuntary movement in organs.
Nervous Tissue:
o Found in the brain, spinal cord, and nerves.
o Conducts electrical impulses.
o Composed of neurons and glial cells.
3. Staining Techniques in Histology
Hematoxylin and Eosin (H&E): Most common, stains nuclei blue and cytoplasm pink.
Periodic Acid-Schiff (PAS): Stains carbohydrates.
Silver Stains: Stains nerve tissues and connective tissues.
Immunohistochemistry: Uses antibodies to detect specific proteins.