Isolation And The Determination Of The Antibiogram Of Listeria Species
Isolated From Fried Poultry Chicken Sold At Chop House And Odokpe
Restaurants In Idah, Kogi State.
PROPOSAL PRESENTATION
BY
ADEGBE DORATHY EDEBO
REG. NO.: FPI/HND/BMB/23/042
DEPARTMENT OF BIOLOGY/MICROBIOLOGY,
NOVEMBER, 2024
1.0 BACKGROUND OF THE STUDY
Foodborne pathogens are microorganisms present in food that lead to illnesses, posing a
significant global challenge (Elbehiry et al., 2023). Foodborne pathogens, such as Listeria
monocytogenes, pose significant health and economic risks, particularly in agricultural, dairy,
and animal-based products (Iacoviello, 2021). Improper hygiene and handling, especially in
restaurant settings, heighten exposure risks. Listeria monocytogenes is notably dangerous,
causing severe illnesses like listeriosis. Fried chicken, a popular dish globally, is a high-risk
food when improperly cooked or stored, emphasizing the need for strict hygiene and proper
temperature management to prevent contamination (Quereda et al., 2021).
1.2 STATEMENT OF THE PROBLEM
According to Guidi et al. (2023), Listeria monocytogenes present a significant threat in poultry
slaughterhouses, endangering consumer health. Listeria monocytogenes, a harmful pathogen,
can cause invasive infections leading to serious health issues, especially in high-risk groups
like pregnant women, older adults, and individuals with weakened immune systems (Quereda,
2021). This highlights the need for a detailed research of the presence and antibiotic resistance
profiles of Listeria species in widely consumed poultry products to inform effective
prevention and control measures (Nakamura, 2020).
1.3 JUSTIFICATION OF THE STUDY
Foodborne illnesses remain a major public health concern in Nigeria, where food safety
regulations are still evolving, and enforcement at local food outlets is inconsistent (Akudo et
al., 2024). This regulatory gap increases the risk of foodborne pathogens like Listeria. Idah,
Kogi State, hosts local eateries such as Chophouse and Odokpe restaurants, which serve as
case studies for assessing these risks. This research investigates the presence and antibiotic
resistance of Listeria in fried poultry sold at these establishments, highlighting the critical
need for strict food safety measures and regular monitoring in similar settings across Nigeria.
1.4 Aim of the Study
To isolate and evaluate the antibiotic resistance patterns of Listeria species from fried poultry
chicken sold at Chop House and Odokpe Restaurants in Idah, Kogi State, to enhance food
safety and public health management.
1.5 Specific Objectives of the Study:
Isolation of Listeria Species: Fried poultry chicken samples from Chop House and Odokpe
Restaurants in Idah, Kogi State, will be analyzed using standard traditional microbiological
technique (Culture method) to detect pathogenic Listeria and identify contamination sources.
Prevalence Assessment: The study will quantify the contamination rate of Listeria species in the
samples, evaluating the associated risk to consumers.
Antibiogram Analysis: Antibiotic resistance patterns of the isolated Listeria species will be
examined to determine their susceptibility and guide effective treatment strategies for infections.
2.0 MATERIALS AND METHOD
Study Area: This study will be conducted in Idah Local Government Area, Kogi State.
Sample Collection: A total of 4 samples (fried poultry chicken) will be collected from Chop
House and Odokpe Restaurants in Idah, Kogi State.
Isolation Techniques: The proposed methodology involves the use of enrichment broths and
selective agar media (e.g., Oxford agar, PALCAM agar).
Identification of Listeria Species: Biochemical tests (e.g., catalase, CAMP) and Gram
staining techniques.
Antibiogram Testing:
Application of the Kirby-Bauer disk diffusion method.
CLSI guidelines for antibiotic susceptibility interpretation.
MATERIALS AND METHOD
Statistical analysis using SPSS.
Evaluation of prevalence rates and resistance patterns.
REFERENCES
Akudo, Q., Ebugosi, Q., & Olaboye, J. (2024). Addressing food and medication quality control challenges in Nigeria: Insights and
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