Faculty of Agirculturural Sciences and AHMAD M.
SALEH
Technology ASSISTANT PROFESSOR
Palestine Technical University-Kadoorie
MOLECULAR MICROBIOLOGY AND
Tulkarm-Palestine BIOTECHNOLOGY
P.O.Box (7)
E-mails: a.m.saleh@ptuk.edu.ps
ahmadsaida@yahoo.com
PERSONAL INFORMATION
NAME: AHMAD MAHMOUD HASAN SALEH
GENDER: MALE
DATE OF BIRTH: 04/05/1974
PLACE OF BIRTH: SAIDA-TULKARM
MARITAL STATUS: MARRIED
NUMBER OF CHILDREN: 4
NATIONALITY: PALESTINIAN
EDUCATIONAL BACKGROUND (ACADEMIC QUALIFICATIONS)
PHD DEGREE
DEGREE: PhD (Dr. rer. nat.)
SPECIALTY: Molecular Microbiology and Biotechnology
RATING: Very Good “ Magna Cum Laude ”
UNIVERSITY: University of Goettingen
CITY/COUNTRY: Goettingen/Germany
YEAR: November 2006.
TYPE OF STUDY: Full-time ( 4 years; Oct. 2002-Nov. 2006)
MAJOR: Microbiology
MINORS: Biochemistry and Genetics
TITLE OF PHD THESIS: Characterization of Alternative NADH Dehydrogenases in the
Respiratory Chain of Toxoplamsa gondii as Novel Drug
Targets.
MASTER DEGREE
DEGREE: M.Sc. (Master of Science)
SPECIALTY: Molecular Biology
RATING: Very Good
G.P.A: 83.2%
RANK: Second
UNIVERSITY: An-Najah National University
CITY/COUNTRY: Nablus/Palestine.
YEAR: June 2001.
TYPE OF STUDY: Full-time ( 4 years; Sep. 1997-June 2001)
MAJOR: Molecular Microbiology
TITLE OF M.SC. THESIS: Molecular Typing of Methicillin Resistant Staphylococcus
aureus (MRSA) by Antibiogram, Ribosomal Spacer and
Arbitrarily Primed-Polymerase Chain Reaction (RS-PCR and
AP-PCR), and Pulsed Field Gel Electrophoresis (PFGE).
B.SC. DEGREE
DEGREE: B.Sc. (Bachelor of Science)
SPECIALITY: Biological Sciences
RATING: Very Good
G.P.A: 81.8%
RANK: FIRST
UNIVERSITY: Yarmouk University
CITY/COUNTRY: Irbid/Jordan
YEAR: June 1997
TYPE OF STUDY: Full-time ( 4 years; Feb. 1993-June 1997)
MAJOR: Biology with focus on microbiology courses.
TITLE OF GRAD. PROJECT: Genotyping of pathogenic Escherichia coli by
Arbitrarily Primed Polymerase Chain Reaction.
TAWJEHI DEGREE
DEGREE: Tawjihi (Higher Secondary School Certificate)
SPECIALITY: Scientific Stream
RATING: Very Good
G.P.A: 88.4%
SCHOOL: Attil Secondary Boys School
CITY/COUNTRY: Attil/Palestine
YEAR: June 1992
WORK EXPERIENCE
PALESTINE TECHNICAL UNIVERSITY (PTUK)/PALESTINE
FACULTY: COLLEGE OF AGRICULTURAL SCIENCES
ADDRESS: TULKARM-PALESTINE
EMPLOYMENT PERIOD: JANUARY 2016 – PRESENT
TITLE: ASSISTANT PROFESSOR
RESPONSIBILITIES:
TEACHING GRADUATE COURSE: MOLECULAR BIOLOGY AND
MICROBIAL BIOTECHNOLOGY
TEACHING UNDERGRADUATES: BIOLOGY 1 AND 2 (THEORY AND PRACTICAL)
MICROBIOLOGY
BIOCHEMISTRY
EDITOR-IN-CHIEF FOR PTUK RESEARCH JOURNAL
UNIVERSITY OF HAI’L/K.S.A.
FACULTY: COLLEGE OF PUBLIC HEALTH
DEPT. OF PUBLIC HEALTH
ADDRESS: Hail, KSA
EMPLOYMENT PERIOD: September 2010 – December 2015
TITLE: ASSISTANT PROFESSOR
RESPONSIBILITIES:
I. Teaching the following courses :
1. PRINCIPLES OF MICROBIOLOGY (THEORY AND PRACTICAL).
2. MOLECULAR DIAGNOSTICS (THEORY AND PRACTICAL).
3. MEDICAL MICROBIOLOGY (THEORY AND PRACTICAL)
4. GENERAL BIOCHEMISTRY (THEORY AND PRACTICAL)
5. ENVIRONMENTAL MICROBIOLOGY (THEORY AND PRACTICAL)
6. PRINCIPLES OF TOXICOLOGY
7. BIOTECHNOLOGY (THEORY AND PRACTICAL)
II. Establishment and furnishing of a new Microbiology teaching lab. as well as an
advanced Molecular Diagnostic Microbiology research lab. at the college (as a Head of the
Laboratories Committee)
III. Participation in the establishment and operating the Medical Diagnostic Microbiology
Labs at the Unit of Molecular Diagnostics and Personalized Medicine (MDxPM) at the
University of Hail as an international medical research center and as a reference labs for the
local hospitals as well as for the forthcoming University Hospital. (Member of the MDxPM
Committee)
IV. Participation in preparation and development of the study plan of the department
(Member of the Curricula Committee)
V. As a member of the Quality Committee at the college, we are working to achieve the
national and international accreditation of our programs. I am responsible for assuring that we
meet the needs of accreditation regarding the teaching resources and infrastructure.
VI. Research:
A. Submitted two research proposals to the KACST for funding in the field of
molecular diagnostic microbiology from clinical and environmental samples (1
proposals has been accepted) and another 2 proposals were submitted and
accepted for funding by the Deanship of Scientific Research at the University of
Hail.
B. Conducting research in a three funded research projects, one is finished and the
other 2 are running.
AL-ISRA PRIVATE UNIVERSITY
FACULTY: Pharmacy and Nursing
ADDRESS: Amman, Jordan
EMPLOYMENT PERIOD: September 2008 – September 2010 (2 Years)
TITLE: ASSISTANT PROFESSOR
RESPONSIBILITIES:
I. Teaching the following courses:
1. GENERAL AND PHARMACEUTICAL MICROBIOLOGY
2. GENERAL AND PHARMACEUTICAL BIOCHEMISTRY
3. PHARMACOGENETICS
4. PHARMACEUTICAL BIOTECHNOLOGY (FOR POSTGRADUATE STUDENTS)
5. GENERAL BIOLOGY
6. NUTRITION
II. Participation in establishment of the new research labs at the college of pharmacy
for the postgraduate program.
III. Conducting research in Pharmacogenetics
MODERN COMMUNITY COLLEGE OF HEALTH SCIENCES
DEPARTMENT: Health Control
EMPLOYMENT PERIOD: September 2007 – August 2008 (1 Year)
TITLE: ASSISTANT PROFESSOR
RESPONSIBILITIES: 1. Teaching the Following courses:
General Microbiology, General Biochemistry.
2. Head of the Dept. of Health Control
INSTIT. OF MEDICAL MICROBIOLOGY, UNIV. OF GOETTINGEN
DEPARTMENT: Medical Bacteriology
ADDRESS: Goettingen, Germany
EMPLOYMENT PERIOD: February 2003 – November 2006 (4 Years)
TITLE: SCIENTIFIC RESEARCHER
RESPONSIBILITIES: 1. Conducting scientific research in the field of molecular
parasitology.
2. Supervision of Master students.
3. Tutoring undergraduate medical students
AL-ISLAMYA SECONDARY PRIVATE SCHOOL
ADDRESS: Nablus, Palestine
EMPLOYMENT PERIOD: September 1997 – September 2002 (5 Years)
TITLE: BIOLOGY TEACHER
RESPONSIBILITIES: 1. Teaching biology for secondary classes and science for
intermediate classes.
2. Conducting training workshops for biology teachers
under the supervision of the ministry of higher
education.
3. Participation in national school exhibitions.
YARMOUK UNIVERSITY, DEPT. OF BIOLOGY
FACULTY: Faculty of Science, Department of Biology
ADDRESS: Irbid, Jordan
EMPLOYMENT PERIOD: June 1996 – June 1997 (1 Year)
TITLE: RESEARCH ASSISTANT
RESPONSIBILITIES: Conducting a scientific research in medical and
molecular microbiology.
PERFORMED RESEARCH PROJECTS
Application f conventional and molecular approaches for investigating the
microbiological quality of drinking water in Hail, KSA.
The Impact of Genetic Polymorphisms in CYP3A5, CYP3A4, and ABCB1 on Trough
Blood Concentrations of Tacrolimus in Jordanian Pediatric Renal Transplants.
PHD PROJECT: Characterization of Alternative NADH Dehydrogenases in the
Respiratory Chain of Toxoplasma gondii as novel drug targets.
M.SC. PROJECT: Molecular Typing of Methicillin Resistant Staphylococcus aureus
(MRSA) by Antibiogram, RS-PCR, AP-PCR and PFGE.
B.SC. GRADUATION PROJECT: Genotyping of Pathogenic Escherichia coli by Different
Molecular Biology Techniques Including PCR, PFGE, and RFLP.
CURRENT PROJECTS, SCHOLARSHIPS, AND AWARDS
CURRENT RESEARCH PROJECTS AND FUNDS:
Prevalence, Genotyping and Antimicrobial Resistance in Helicobacter pylori isolated
from patients in Hail.
Role: Principal Investigator
Submitted to: KACST ( King Abdulazziz City for Science and Technology)
Budget: 1,500,000 SR (1.5 Millions)
Status: Preliminary Accepted
Identification of Salmonella spp. in poultry and their products in Hail by using
conventional and molecular methods.
Role: Principal Investigator
Submitted to: KACST (King Abdulazziz City for Science and Technology)
Budget: 100,000 SR
Status: Done
Identification of Human Pathogenic Strains of Shiga Toxin Escherichia coli from Food
and Clinical Samples Isolated from Hail Region by Molecular Typing of Shiga Toxin
Genes.
Role: Principal Investigator
Submitted to: University of Hail in 2014
Budget: 80000 SR
Status: Running
Microbiological, Molecular identification and antimicrobial resistance of E. coli in
drinking water collected from Hail.
Role: Co-Investigator
Submitted to: University of Hail in 2014
Budget: 85000 SR
Status: Done
PREVIOUS GRANTS, SCHOLARSHIPS AND AWARDS:
A grant of 7500 JD (1000 USD) from Al-Isra Private University to conduct a research
project in the field of Pharmacogentics.
Full scholarship awarded by the "Deutscher Akademischer Austausch Dienst" –DAAD-
(German Academic Exchange Service - Bonn) for pursing postgraduate studies towards
the PhD degree in Germany (2002-2006).
PATENT for a new anti-malarial and anti-toxoplasmosis drug (HDQ) registered by the
University of Goettingen, Germany.
Annual honor awards from Dept. of Biology, Yarmouk University (1993-1997).
RESEARCH AND TECHNICAL SKILLS
RESEARCH :
Gene cloning; DNA sequencing; Recombinant protein expression and purification;
Immunization and polyclonal antibody production; Western, southern and northern blotting;
Transfection; Tissue culturing (Culturing of Toxoplasma gondii and Plasmodium
falciparum), Immunofluorescence staining and microscopy; Gene silencing by antisense
RNA; Different types of conventional PCR applications; RT-PCR; Quantitative gene
expression analysis by light-cycler (real time RT-PCR); Biochemical analysis of enzymatic
activity; Drug inhibition assays, and other related molecular biology techniques.
Microbial identification and antimicrobial resistance by using Micrscan Talkaway, MALDI-
TOF-MS Biotyper, the CheckPoints Microarray, QIAcube for automated DNA and RNA
extraction, QIAxel for capillary electrophoresis.
COMPUTER:
Windows XP, Microsoft Office Package (Word, Excel, Power Point, Access, …etc),
Statistical analysis softwares (SPSS, PRISMA ), Bioinformatics (PHYLIP, DNAStar, Clone
manager, Enhance, multiple alignments software).
TEACHING:
Using computer based teaching methods (SMARTBOARD) which include preparation
lecture notes on power point presentations with colored illustrations, figures, videos, and
animations. Using the internet resources for supporting the taught material, and contacting
with students via the internet to access handouts, assignments, and homeworks.
ATTENDED CONFERENCES AND WORKSHOPS
1. Workshop for developing skills needed for writing scientific research proposals by the
KACST. University of Hail, 05/02/2013.
2. The 3rd Scientific day, Philadelphia Private University, 06 January 2009, Amman,
Jordan. (Abstract and Presentation)
3. The 2nd Jordanian Egyptian Conference of Biotechnology: Prospects and Challenges,
Nov. 11-13 , 2008, Yarmouk University, Jordan. (Abstract and Presentation)
4. The 5th Palestinian Conference of Lab. Medicine. 28-29 March 2008 Arab American
University, Jenin, Palestine. (Abstract and Presentation)
5. International Conference: Cell Organelles of Protozoan Parasites: From Basic Science to
Drug Targets, Prague 5-7 June 2006. (Poster and Presentation)
6. Cost Action 857 3rd Annual Workshop: Apicomplexan Biology in Post-genomic Era,17-
20 May 2006, Dresden, Germany (Abstract and Presentation)
7. International Congress of Parasitology, 23-25 March 2006, Vienna, Austria. ( Poster)
8. 2nd Short Course of Young Parasitologists, 19-22 March 2006, Vienna. (Presentation)
9. International Conference of Candidosis (IFOCAN 2005), 23-25 September 2005
Goettingen, Germany.
10. International Annual Congress of the DGHM and VAAM, 25-28 September 2005
Goettingen, Germany. (Abstract and Poster)
11. Workshop of Molecular Biology Techniques at Bethlehem University organized by
UNESCO, October 2000.
RESEARCH INTERESTS
Molecular identification, genotyping, and antibiotic resistance of pathogenic bacteria in
environmental and clinical samples.
Impact of inter-individual genetic polymorphisms in genes of drug metabolizing enzymes
on drug response and disease risk.
Prevalence of genetic variations correlated with human genetic disorders in the Arab
population.
Studying the antimicrobial potential of herbs used in the local public medicine.
PUBLICATIONS
1. Prevalence and antimicrobial resistance of Salmonella spp. isolated from chicken
portions collected from local markets of Hail city. ( Submitted)
2. Application of conventional and molecular approaches for investigating the
microbiological quality of drinking water in Hail. Saudi Journal of Biological
Sciences. (In preparation)
3. Lin SS, Kerscher S, Saleh A, Brandt U, Gross U, Bohne W. The Toxoplasma
gondii type-II NADH dehydrogenase TgNDH2-I is inhibited by 1-hydroxy-2-
alkyl-4(1H)quinolones. Biochim Biophys Acta. 2008 Nov;1777(11):1455-62.
http://www.ncbi.nlm.nih.gov/pubmed/18786503
4. Saleh A, Friesen J, Baumeister S, Gross U, Bohne W. Growth inhibition of
Toxoplasma gondii and Plasmodium falciparum by nanomolar concentrations of
1-hydroxy-2-dodecyl-4(1H)quinolone, a high-affinity inhibitor of alternative
(type II) NADH dehydrogenases. Antimicrob Agents Chemother. 2007
Apr;51(4):1217-22. http://www.ncbi.nlm.nih.gov/pubmed/17242151
5. Saleh A, Bohne W. Characterization and gene sequencing of mitochondrial
alternative NADH dehydrogenase 1 in Toxoplasma gondii. NCBI GenBank
ABB17192.1 http://www.ncbi.nlm.nih.gov/protein/ABB17192.1
6. Saleh A, Bohne W. Characterization and gene sequencing of mitochondrial
alternative NADH dehydrogenase 2 in Toxoplasma gondii. NCBI GenBank
ABA44355.1 http://www.ncbi.nlm.nih.gov/protein/ABA44355.1
7. Saleh A, Bohne W. Gene sequencing of F1-ATP synthase beta subunit in the
mitochondria of Toxoplasma gondii. NCBI GenBank ABB17195.1
http://www.ncbi.nlm.nih.gov/protein/ABB17195.1
8. Saleh A, Bohne W. Gene sequencing of mitochondrial cytochrome c1 in the
respiratory chain of Toxoplasma gondii. NCBI GenBank ABB17194.1
http://www.ncbi.nlm.nih.gov/protein/ABB17194.1
9. Saleh A, Bohne W. Sequence and gene structure of flavoprotein subunit of
succinate dehydrogenase in the respiratory chain of Toxoplasma gondii. NCBI
GenBank ABB17193.1. http://www.ncbi.nlm.nih.gov/protein/ABB17193.1
10. Tamer ESSAWI, Kamel ADWAN, Nael ABU-HASSAN, Galeb ADWAN,
Ahmed SALEH. Typing of Methicillin-Resistant Staphylococcus aureus
(MRSA) By Ribosome Spacer and Arbitrarily Primed Polymerase Chain
Reaction. Turk J Med Sci 34 (2004) 5-9
http://staff.najah.edu/sites/default/files/Typing_Of_Methicillin-
Resistant_Staphylococcus_Aureus_By_Ribosome_Spacer_And_Arbitrarily_Prim
ed_Polymerase_Chain_Reaction.pdf
SCIENTIFIC MEMBERSHIPS
The Arab Molecular Biology Organization.
German Society of Parasitology (DGP: Deutsche Gesellschaft fuer Parasitologie),
Stuttgart, Germany from 2003 – Now.
German Foundation of Hygiene and Microbiology (DGHM), Germany from 2003 – Now
National Jordanian Society of Biotechnology, Amman-Jordan from 2005 – Now
LANGUAGES
English : Speaking , reading, writing and understanding: very good.
(TOEFL score: 550; 213 computer based test).
German: 6 months of intensive language courses (3 levels) at Goethe Institute of
Goettingen, Germany ended with a DSH certificate (Rating: very good)
REFERENCES
1. Dr. Nael Abu Hassan, PhD Biochemistry; Biology Department, An-Najah N. University,
Nablus-Palestine. Mobile: 0599265620 Email: nsabuhasan@najah.edu (MSc
Supervisor)
2. Dr. Kamel Adwan, PhD Microbiology; Biology Department, An-Najah N. University,
Nablus-Palestine. Mobile: 0598559024 E-mail: adwank@yahoo.com (MSc Supervisor)
3. Dr. Mazen Salman, PhD in Phytopathology, PTUK, Palestine. Mobile +970-(0)-598-
491-413, salman_mazen@daad-alumni.de
4. Dr. Belal Rahhal, PhD in Physiology, Faculty of Pharmacy, An-Najah N. University,
Nablus-Palestine. Mobile: 0599 362 853 Email: belalrahhal@najah.edu