URIC ACID
Mono liquid
Cat.No Package Sizes REFERENCE VALUES
Serum/Plasma
UA450S45 4 x 50 mL + Standard (3 mL)
Female Male
UA4100S83 4 x 100 mL + Standard (3 mL) mg/dl (µmol/l) mg/dl (µmol/l)
UA2125S144 2 x 125 mL + Standard (3 mL) Adults 2,3–6,1 (137–363) 3,6-8,2 (214-488)
UA200H145 Hit I 4 x 50 mL Children
0 – 5 Days 1,9-7,9 (113-470) 1,9-7,9 (113-470)
UA400H245 Hit II 4 x 100 mL
1 – 4 Years 1,7-5,1 (101-303) 2,2-5,7 (131-340)
5 – 11 Years 3,0-6,4 (178-381) 3,0-6,4 (178-381)
METHOD 12 – 14 Years 3,2-6,1 (190-363) 3,2-7,4 (190-440)
15 – 17 Years 3,2-6,4 (190-381) 4,5-8,1 (268-482)
Enzymatic – colorimetric. Trinder, End Point.
Urine
Uricase -PAP
≤ 800 mg/d (4,76 mmol/d) with normal food
≤ 600 mg/d (3.57 mmol/d) with reduced purine food
PRINCIPLE Note: It is recommended for each laboratory to establish and
Enzymatic determination of uric acid according to maintain its own reference values. The given data here are only
the following reactions: an indication.
UricAcid + H2O + O2 Uricase> Allantoin + CO2 + H2O2 PROCEDURE
This reagent can be used manually (see method
TBHBA+ 4-Aminoantipyrin + 2 H2O2 POD> Chinonimine+ 3H2O
below) and on most analyzers. The applications are
(TBHBA= 2,4,6-Tribrom3-hydroxybenzoic acid) available on request.
Wavelength : 550 nm
REAGENT COMPOSITION Temperature : 37°C
R1: Phosphatebuffer pH 7,0 100 mmol/l Cuvette : 1 cm light path
TBHBA 1 mmol/l
4-Aminoantipyrine 0,3 mmol/l
K4[Fe(CN)6] 10 µmol/l Read against reagent blank (RBL)
Peroxidase (POD) ≥ 2 kU/l .
Uricase ≥ 30 U/l • Monoreagent procedure
Standard: 6 mg/dl (357 µmol/l)
Monoreagent 1 mL
PRECAUTIONS Sample 20 µL
- For in vitro diagnostic use only. Mix and after a 5 minutes incubation, measure the
- The reagent contains less than 0,95g/L sodium change of absorbance (A) within 60 minutes against
azide. Avoid contact with skin and/or mucous reagent blank
membranes !
- Use clean or single use glass material only to
avoid contaminations. CALCULATION
A Sample mg/L C = 60
STABILITY OF REAGENT xC mg/dL C = 6
A Standard µmol/L C = 357
When stored at 2-8° C and protected from light,
the unopened reagent and standard are stable until C = standard concentration.
the expiry date printed on the labels .
Take the dilution factor into account for the calculation of
Reagent and standard are ready for use. uric acid concentration in urine!
Stability after opening the bottles :
3months at 2 – 8 °C
4 weeks at 18 – 25 °C
(contamination has to be avoided !)
SAMPLE
Serum free of hemolysis , plasma (EDTA/heparine)
Urine diluted 1/10 in distilled water.
L-S 04/2009 page 1/2
CALIBRATORS & CONTROLS BIBLIOGRAPHY
For calibration Inmesco Multicalibrator Introcal may
st
be used alternatively , for quality control use 1. Thomas L. Clinical Laboratory Diagnostics. 1
Inmesco normal and abnormal control, ed. Frankfurt: TH-Books Verlagsgesellschaft;
Intronorm I and Intronorm II . 1998. p. 208-14.
2. Newman DJ, Price CP. Renal function and
nitrogen metabolites. In: Burtis CA, Ashwood
PERFORMANCE DATA ER, editors. Tietz Textbook of Clinical
rd
- Analytical range Chemistry. 3 ed. Philadelphia: W.B Saunders
The reagent is linear from 0,07 – 20 mg/dl (4,2 - Company; 1999. p. 1204-70.
1190 µmol/l)
- Detection limit
The detection limit is equal to 0,07 mg/dl (4,2 µmol/l)
-
Precision SYMBOLS USED
Within-run reproducibility
n = 20
Mean SD CV Unit For in vitro diagnostic medical use
Control 1 4,37 0,021 0,49 mg/dl
Control 2 10,74 0,032 0,30 mg/dl
Patient 5,46 0,044 0,81 mg/dl Batch Code
Between-run reproducibility
n = 20 Use by
Mean SD CV Unit
Control 1 4,42 0,037 0,84 mg/dl
Temperature limitation
Control 2 10,82 0,058 0,54 mg/dl
Patient 5,41 0,051 0,99 mg/dl
- Correlation
A comparative study has been performed between
the Inmesco method and another commercial
reagent on 70 human serum samples.
The parameters of linear regression are as follows:
y = 0,948 x + 0,44 mg/dl r= 0,995
INTERFERENCES
Interferences are found according to literature
L-S 04/2009 page 2/2
