URIC ACID

5+1 liquid

Cat.No Package Size REFERENCE VALUES

Serum/Plasma
UA460S46 4 x 50 mL 2 x 20 mL + Std
Female Male
UA4120S82 4 x 100 mL 1 x 80 mL + Std mg/dl (µmol/l) mg/dl (µmol/l)
UA240H146 Hit I 4 x 50 mL 2 x 20 mL Adults 2,3–6,1 (137–363) 3,6-8,2 (214-488)
UA480H246 Hit II 4 x 100 mL 4 x 20 mL Children
0 – 5 Days 1,9-7,9 (113-470) 1,9-7,9 (113-470)
UA492H746 Hit 917 6 x 66 mL 6 x 16 mL
1 – 4 Years 1,7-5,1 (101-303) 2,2-5,7 (131-340)
5 – 11 Years 3,0-6,4 (178-381) 3,0-6,4 (178-381)
METHOD
12 – 14 Years 3,2-6,1 (190-363) 3,2-7,4 (190-440)
Enzymatic – colorimetric. Trinder, End Point. 15 – 17 Years 3,2-6,4 (190-381) 4,5-8,1 (268-482)
Uricase-PAP Urine
≤ 800 mg/d (4,76 mmol/d) with normal food
PRINCIPLE ≤ 600 mg/d (3.57 mmol/d) with reduced purine food
Enzymatic determination of uric acid according to Note: It is recommended for each laboratory to establish and
the following reactions: maintain its own reference values. The given data here are only
an indication.
UricAcid + H2O + O2 Uricase> Allantoin + CO2 + H2O2
PROCEDURE
TBHBA+ 4-Aminoantipyrin + 2 H2O2 POD> Chinonimine+ 3H2O This reagent can be used manually (see method
(TBHBA= 2,4,6-Tribrom3-hydroxybenzoic acid) below) and on most analyzers. The applications are
available on request.
REAGENTS Wavelength : 550 nm
Reagent Composition Temperature : 37°C
(concentrations in the test) Cuvette : 1 cm light path

R1: Phosphatebuffer pH 7,0 100 mmol/l Read against reagent blank (RBL)
TBHBA (2,4,6-Tribrom- 1 mmol/l (for RBL mix R1 + R2 = 5 + 1 )
3-hydroxybenzoicacid • Two-reagent procedure
R2: Phosphatebuffer pH 7,0 100 mmol/l
4-Aminoantipyrine 0,3 mmol/l
K4[Fe(CN)6] 10 µmol/l R1 1 mL
Peroxidase (POD) ≥ 2 kU/l Sample/Standard 20 µL
Uricase ≥ 30 U/l Mix and incubate for 5 minutes, then add
Standard: 6 mg/dl (357 µmol/l)
R2 200 µL
PRECAUTIONS
- For in vitro diagnostic use only. Mix and after a 5 minutes incubation, measure the
- The reagent contains < 0,95 g/L sodium azide. change of absorbance A within 60 minutes against
Avoid contact with skin and/or mucous reagent blank
membranes ! • Monoreagent procedure
- Use clean or single use glass/plastic material Mix R1 + R2 = 5 + 1 => Monoreagent
Stability 3 months at 2 – 8°C
only to avoid contaminations.
Monoreagent 1 mL
STABILITY OF REAGENT Sample/Standard 20 µL
When stored at 2 - 8° C and protected from light, Mix and after a 5 minutes incubation, measure the
the reagents are stable until the expiry date stated change of absorbance A within 60 minutes against
on the label. reagent blank

PREPARATION OF WORKING REAGENT CALCULATION

The reagents are ready for use. A Sample mg/L C = 60
x C mg/dL C = 6
A Standard
SAMPLE µmol/L C = 357
Serum free of hemolysis , plasma (EDTA/heparine) C = standard concentration.
Take the dilution factor into account for the calculation of
Urine diluted 1/10 in distilled water. uric acid concentration in urine.

L-S 04/2009 page 1/2

CALIBRATORS & CONTROLS BIBLIOGRAPHY
For calibration Inmesco Multicalibrator Introcal may
st
be used alternatively , for quality control use 1. Thomas L. Clinical Laboratory Diagnostics. 1
Inmesco normal and abnormal control, Intronorm I ed. Frankfurt: TH-Books Verlagsgesellschaft;
and Intronorm II . 1998. p. 208-14.
2. Newman DJ, Price CP. Renal function and
PERFORMANCE DATA nitrogen metabolites. In: Burtis CA, Ashwood
- Analytical range ER, editors. Tietz Textbook of Clinical
rd
The reagent is linear up to 20 mg/dl (1190 µmol/l) Chemistry. 3 ed. Philadelphia: W.B Saunders
- Detection limit Company; 1999. p. 1204-70.
The detection limit is equal to 0,07 mg/dl (4,2 µmol/l)

Precision
Within-run reproducibility SYMBOLS USED
n = 20
Mean SD CV Unit
Control 1 2,75 0,04 1,55 mg/dl For in vitro diagnostic medical use
Control 2 5,35 0,04 0,74 mg/dl
Patient 10,1 0,08 0,77 mg/dl
Batch Code
Between-run reproducibility
n = 20
Mean SD CV Unit Use by
Control 1 2,68 0,04 1,52 mg/dl
Control 2 5,23 0,09 1,63 mg/dl Temperature limitation
Patient 9,98 0,11 1,06 mg/dl

- Correlation
A comparative study has been performed between
the Inmesco method and another commercial
reagent on 70 human serum samples.
The parameters of linear regression are as follows:

y = 1,02 x – 0,44 mg/dl r = 0.997

INTERFERENCES

Interfences are according to literature

L-S 04/2009 page 2/2