Cambridge IGCSE™
BIOLOGY 0610/52
Paper 5 Practical Test October/November 2024
CONFIDENTIAL INSTRUCTIONS
* 6 7 1 0 3 4 3 8 3 3 *
This document gives details of how to prepare for and administer the practical exam.
The information in this document and the identity of any materials supplied by Cambridge International
are confidential and must NOT reach candidates either directly or indirectly.
The supervisor must complete the report at the end of this document and return it with the scripts.
INSTRUCTIONS
● If you have any queries regarding these confidential instructions, contact Cambridge International
stating the centre number, the syllabus and component number and the nature of the query.
email info@cambridgeinternational.org
phone +44 1223 553554
This document has 8 pages. Any blank pages are indicated.
DC (PB (GB)) 336148/2
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General information about practical exams
Centres must follow the guidance on science practical exams given in the Cambridge Handbook.
Safety
Supervisors must follow national and local regulations relating to safety and first aid.
Only those procedures described in the question paper should be attempted.
Supervisors must inform candidates that materials and apparatus used in the exam should be treated
with caution. Suitable eye protection should be used where necessary.
The following hazard codes are used in these confidential instructions, where relevant:
C corrosive MH moderate hazard
HH health hazard T acutely toxic
F flammable O oxidising
N hazardous to the aquatic environment
Hazard data sheets relating to substances used in this exam should be available from your chemical
supplier.
Before the exam
● The packets containing the question papers must not be opened before the exam.
● It is assumed that standard school laboratory facilities, as indicated in the Guide to Planning
Practical Science, will be available.
● Spare materials and apparatus for the tasks set must be available for candidates, if required.
During the exam
● It must be made clear to candidates at the start of the exam that they may request spare materials
and apparatus for the tasks set.
● Where specified, the supervisor must perform the experiments and record the results as instructed.
This must be done out of sight of the candidates, using the same materials and apparatus as the
candidates.
● Any assistance provided to candidates must be recorded in the supervisor’s report.
● If any materials or apparatus need to be replaced, for example, in the event of breakage or loss,
this must be recorded in the supervisor’s report.
After the exam
● The supervisor must complete a report for each practical session held and each laboratory used.
● Each packet of scripts returned to Cambridge International must contain the following items:
– the scripts of the candidates specified on the bar code label provided
– the supervisor’s results relevant to these candidates
– the supervisor’s reports relevant to these candidates
– seating plans for each practical session, referring to each candidate by candidate number
– the attendance register.
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Specific information for this practical exam
During the exam, the supervisor (not the invigilator) must do the experiment in Question 1 and
record the results on a spare copy of the question paper, clearly labelled ‘supervisor’s results’.
Question 1
Each candidate should be provided with:
quantity per
hazard materials and apparatus
candidate
spinach leaves or equivalent 8
mortar and pestle (If unavailable a heavyweight ceramic bowl and a
1
metal teaspoon can be used.)
cold isolation medium in a 100 cm3 beaker labelled isolation medium 50 cm3
small funnel 1
bench lamp with a 60 watt or equivalent bulb 1
standard test‑tubes (approximately 125 mm × 15 mm) 2
test‑tube rack to hold standard test‑tubes 1
a supply of ice‑cold water to be distributed to candidates when they
raise their hand (This can either be made using ice and water or by 50 cm3
keeping the water in the refrigerator prior to the practical.)
permanent marker pen 1
stop‑clock 1
1 cm3 syringe 1
5 cm3 syringe 1
20 cm × 20 cm pieces of muslin (If larger funnels are used, then bigger
muslin may be required. Check that the muslin is sufficiently large to 3
reach the edges of the funnel.)
[HH], [MH], DCPIP in a 100 cm3 beaker labelled DCPIP
50 cm3
[N]
empty 100 cm3 beaker labelled water‑bath 1
empty 250 cm3 beaker labelled chloroplast suspension 1
paper towels 5
gloves 1 pair
suitable eye protection 1
container labelled waste that is large enough to hold the funnel 1
30 cm ruler with a mm scale (also needed for Question 2) 1
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Preparation of materials
Phosphate buffer solution
Dissolve 4.48 g of hydrated disodium hydrogen phosphate (Na2HPO4•12H2O) and 1.7 g of potassium
dihydrogen phosphate (KH2PO4) in 500 cm3 of distilled water. Keep refrigerated until required.
Isolation medium
Dissolve 68.46 g of sucrose and 0.38 g of potassium chloride (KCl ) in phosphate buffer solution and
make up to 500 cm3 with phosphate buffer solution. Keep refrigerated until just before the exam.
DCPIP solution [HH], [MH], [N]
Dissolve 0.01 g of DCPIP and 0.93 g of KCl in phosphate buffer solution and make up to 500 cm3 with
phosphate buffer solution. Keep refrigerated until required.
Spinach leaves
Common spinach (Spinacia oleracea) leaves are suitable and should be fresh. If fresh spinach is not
available an equivalent quantity of fresh green lettuce (such as iceberg) or cabbage leaves can be
used as a substitute. Ensure that the substituted leaves are of approximately the same total size as
eight spinach leaves.
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Permission to reproduce items where third‑party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
To avoid the issue of disclosure of answer‑related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.
Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.
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Supervisor’s report
Syllabus and component number /
Centre number
Centre name ............................................................................................................................................
Time of the practical session ...................................................................................................................
Laboratory name/number ........................................................................................................................
Give details of any difficulties experienced by the centre or by candidates (include the relevant
candidate names and candidate numbers).
You must include:
● any difficulties experienced by the centre in the preparation of materials
● any difficulties experienced by candidates, e.g. due to faulty materials or apparatus
● any specific assistance given to candidates.
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Declaration
1 Each packet that I am returning to Cambridge International contains all of the following items:
● the scripts of the candidates specified on the bar code label provided
● the supervisor’s results relevant to these candidates
● the supervisor’s reports relevant to these candidates
● seating plans for each practical session, referring to each candidate by candidate number
● the attendance register.
2 Where the practical exam has taken place in more than one practical session, I have clearly
labelled the supervisor’s results, supervisor’s reports and seating plans with the time and laboratory
name/number for each practical session.
3 I have included details of difficulties relating to each practical session experienced by the centre or
by candidates.
4 I have reported any other adverse circumstances affecting candidates, e.g. illness, bereavement or
temporary injury, directly to Cambridge International on a special consideration form.
Signed ................................................................................................................................. (supervisor)
Name (in block capitals) ..........................................................................................................................
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