Service Manual

Automatic Chemistry Analyzer

CONTENT
S
CONTENTS......................................................................................I

1. Product Specifications...........................................................1-1
1.1. Configuration of equipment units..........................................................1-1
1.2. General specifications............................................................................1-2
2. Flow Path Diagrams...............................................................2-1
2.1. Functions of pump, valve.......................................................................2-2
3. Motors, Detectors, Fuses and Solenoid Valves........................3-1
3.1. List of Motors......................................................................................... 3-1
3.2. List of Detectors..................................................................................... 3-1
3.3. List of Fuses........................................................................................... 3-2
3.4. Sensor Logics......................................................................................... 3-2
3.5. List of Solenoid Valves, Pumps and Heaters..........................................3-4
4. Cross Wiring Reference..........................................................4-1
4.1. Motors.................................................................................................... 4-1
4.2. Detectors............................................................................................... 4-2
4.3. Solenoid Valves, Pumps, LEDs...............................................................4-4
5. Schematic Circuit Diagrams...................................................5-1
5.1. List of Boards......................................................................................... 5-1
5.2. Electrical System Block Diagram...........................................................5-2
5.3. Communication board............................................................................5-3
5.3.1. Component Layout Diagram......................................................5-3
5.3.2. Description of LED functions and test Points.............................5-4
5.3.3. Software update.........................................................................5-4
5.4. Main Board........................................................................................... 5-13
5.4.1. Functions................................................................................. 5-13
5.4.2. Component Layout Diagram....................................................5-14
5.4.3. Description of LED functions and test Points...........................5-15
5.5. Motor Board......................................................................................... 5-17
5.5.1. Functions................................................................................. 5-17
5.5.2. Component Layout Diagram....................................................5-18
5.5.3. Description of LED functions and test Points...........................5-19
5.6. SW Board............................................................................................. 5-21
5.6.1. Functions................................................................................. 5-21
5.6.2. Component Layout Diagram....................................................5-21

I
 5.7. Liquid Level Detector Board.................................................................5-23
5.7.1. Functions................................................................................. 5-23
5.7.2. Component Layout Diagram....................................................5-23
5.7.3. Description of LED functions and test Points...........................5-24
5.8. LQF Board............................................................................................ 5-25
5.8.1. Function................................................................................... 5-25
5.8.2. Component Layout Diagram....................................................5-25
5.8.3. Description of LED functions and test point.............................5-25
5.9. Mixing Motor optocoupler board..........................................................5-26
5.9.1. Functions................................................................................. 5-26
5.9.2. Component Layout Diagram....................................................5-26
5.9.3. Description of LED functions and test point.............................5-26
5.10. CoolCtr Board....................................................................................... 5-27
5.10.1. Functions................................................................................. 5-27
5.10.2. Component Layout Diagram....................................................5-27
5.10.3. Description of LED functions and test Points...........................5-28
6. Adjustment Specifications......................................................6-1
6.1. Reaction disk adjustment.......................................................................6-1
6.2. Reagent arm adjustment.......................................................................6-2
6.3. Mixing arm adjustment..........................................................................6-5
6.4. Clean arm adjustment...........................................................................6-6
6.5. Lamp position adjustment......................................................................6-7
6.6. Water Blank(AD) value adjustment........................................................6-8
7. Instrument malfunction and alarm handling...........................7-1
7.1. Details of Alarm..................................................................................... 7-1
7.2. Troubleshooting..................................................................................... 7-3
7.2.1. XX cup absorbance error...........................................................7-6
7.2.2. Reagent/Sample probe drop water............................................7-7
7.2.3. Liquid level detection exception................................................7-7
7.2.4. Communication failed................................................................7-8
7.2.5. Reaction disk’s optocoupler error..............................................7-8
7.2.6. Reagent disk’s optical coupling error.........................................7-9
7.2.7. No water from the sample&reagent probe................................7-9
7.2.8. Reaction cup overflow..............................................................7-10
8. Maintenance......................................................................... 8-1

II
1. Product Specifications

1.1. Configuration of equipment units

1
1.2. General specifications

Item Details
Throughput 150 tests/hour
Number of simultaneously analyzed
40 max
tests
Analytical methods 1 point end; 2 point end; Fixed-time; kinetic
1 point linear; 2 point linear; Exponential;
Calibration methods Logit-log 3p; Logit-log 4P; Logit-log 5P;
Spline; Line graph
Required sample volume 2 to 30μL /test(0.1μL unit)
Required reagent volume 20-300μL /test(0.1μL unit)
Sample positions 40
R2: 40; R1: 40 (One position is exclusively
Reagent positions
for Diluent)
Detection of sample and reagent Possible with electrostatic type liquid level
insufficiency sensor
Reaction table 44 cuvettes
Material: Special plastic
Reaction cuvette Shape: 6 x 5 rectangular shape (optical path
length 5 mm)
Reaction time 10 minutes
Reaction temperature 37℃
Immediately after sample or reagent 2 is
Mixing time
pipetted
Multi-wavelength after-diffraction
Photometer system
photometer
8 wavelength fixed; 4 wavelength for
Wavelength
optional
Direct measurement of reaction cuvette,
Photometric system single wavelength or double wavelength
simultaneous measurement
Minimum volume required for
150μL
photometry
Optical Path length 6 mm
Light source Halogen tungsten lamp
System interface Network

2
2. Flow Path Diagrams

1
1.3. Functions of pump, valve

SV,P
No. Class Function
No.
1 Pump P1 Aspirate DI water for dispending probe inner wall
2 Pump Aspirate DI water for rinse bath, washing probe 1 and
P2
washing probe 2.
3 Pump P3-1 Aspirate waste water of waste water probe 1
4 Pump P3-2 Aspirate waste water of waste water probe 2
5 Pump P3-3 Aspirate waste water of waste water probe 3
6 Solenoid
V1 Water supply for syringe
valve
7 Solenoid
V21 Water supply for dispending probe rinse bath
valve
8 Solenoid
V22 Water supply for mixing rinse bath
valve
9 Solenoid
V23-1 Water supply for washing probe 1
valve
10 Solenoid
V23-2 Water supply for washing probe 2
valve

2
3. Motors, Detectors, Fuses and Solenoid
Valves

1.4. List of Motors

Outline of Motor Control
No Name of mechanism
motor(mm) Board

M1 Sampling arm up/down 35*35*40 Motor Board

M2 Mixing arm up/down 35*35*40 Motor Board

M3 Sample Syringe 42*42*40 Motor Board

Sampling arm/ Mixing arm
M4 42*42*42 Motor Board
horizontal moving
M5 NC

M6 Clean arm up/down 57*57*55 Motor Board

M7 Sample & Reagent Disk 57*57*55 Motor Board

M8 Reaction table 57*57*55 Main Board

M9 Mixing motor (DC motor) 22*16 Main Board

1.5. List of Detectors

Model Detectio
No Class Application
Name n
S1 Photointerrupter Sample probe upper limit point Gk105 Dark

S2 Photointerrupter Mixing upper limit point Gk105 Dark

S3 Photointerrupter Sample syringe upper limit point Gk105 Dark

Sample arm/ Mixing arm horizontal
S4 Photointerrupter Gk105 Dark
home position
S5 Photointerrupter NC Gk105 Dark

S6 Photointerrupter Clean arm upper limit point Gk105 Dark

Sample & Reagent disk rotates to
S7 Photointerrupter Gk105 Dark
home position
S8 Photointerrupter Reaction table count/home Gk105 Bright
S9 Temp Sensor Reaction table temperature sensor NTC 10K
Detect whether Deionized water is Float Low level
S10 IO Sensor insufficient or waste water is full switch /break off

1
1.6. List of Fuses

The following shows the fuses used in equipment. When replacing them, insert
new fuses with correct ratings, referring to the rating indications near their
installation places.

Fuse No Model Rating(A) Connection load

F1 AC socket 5 The cooler power and the main power

F2 AC socket 5 The cooler power and the main power

1.7. Sensor Logics

Outpu
Function Status when t Inversion
Unit name Sensor
Sensor sensed on board
Signal
H: C ≫ Co
Liquid when there
level Electrostatic L
is liquid
detection capacitance
Sample arm Dark at upper
Upper limit H Inverted
up/down limit
point Photointerrupter point
Faulty Photointerrupter L
descent Bright at
collision
Sample arm/
Mixing arm HP, cell Inverted
Photointerrupter Dark at HP H
Horizontal position
moving
Bright at HP
L
Counts changes
L Inverted
from
Reaction HP during
Dark to Bright Inverted
table Cell count Photointerrupter count
ADC starts Inverted
Rotation ADC start L
when Dark
during
changes to
start
Bright
HP
Reagent disk Stop Photointerrupter Bright at HP L Inverted
Rotation position
count
Mixing Upper limit Dark at upper Inverted
Photointerrupter H
up/down point limit point
Upper limit Dark at upper Inverted
Syringe Photointerrupter H
point limit point
2
Rinse Upper limit Dark at upper Inverted
Photointerrupter H
up/down point limit point
Waste
solution Detection
ON－OFF ON when full L
with full
Amount

Deionized Water
ON－OFF OFF with no
water present or L
liquid
Amount Absent

3
1.8. List of Solenoid Valves, Pumps and Heaters

N SV, P Applicatio
Class Type AC/DC NO/NC
o No. n
Solenoi
1 V1 2-port DC24V N.C
d valve
Solenoi
2 V21 2-port DC24V N.C
d valve
Solenoi
3 V22 2-port DC24V N.C
d valve
2-port V23-1 to V23-
V23-1
Solenoi 3 are connected
4 to DC24V N.C
d valve with the same
V23-3
power supply.
DI Water
5 Pump P1 2-port DC24V N.C
supply
DI Water
6 Pump P2 2-port DC24V N.C
supply
2-port P3-1 to P3-4
Waste
P3-1 to are connected with
7 Pump DC24V water N.C
P3-4 the same power
Discharge
supply.
Incubation
bath
9 Heater Ht1 DC24V constant N.C
temperatur
e

4
4. Cross Wiring Reference
1.9. Motors

RED U1_1 driver_IC

GREEN 1 43 6
M 2 35 14
BLUE 3 33 15 U2_1
Sample Arm Up/Down BLACK 4 23 13 45
POW
M1 44
STEP
M1 41
DIR
BLACK U1_2 driver_IC
GREEN 1 43 6 39
POW
M 2 35 14 38
STEP
Sample Arm Rotate RED 3 33 15 37
DIR
BLUE 4 23 13
M2 33
POW
M2 30
STEP
RED U1_3 driver_IC 29
DIR
GREEN 1 43 6
Syringe Motor push/pull M 2 35 14 MCU
BLUE 3 33 15
BLACK 4 23 13
M3
M3

RED U1_4 driver_IC

GREEN 1 43 6
Stirrer Arm Up/Down M 2 35 14
BLUE 3 33 15 U2_2
BLACK 4 23 13 45
POW
M4 44
STEP
M4 41
DIR
BLACK U1_5 driver_IC
GREEN 1 43 6 39
POW
Stirrer Arm Rotate M 2 35 14 38
STEP
RED 3 33 15 37
DIR
BLUE 4 23 13
M5 33
POW
M5 30
STEP
RED U1_6 driver_IC 29
DIR
GREEN 1 43 6
Clean Arm Up/Down M 2 35 14
BLUE 3 33 15 MCU
BLACK 4 23 13
M6
M6

RED U1_7 driver_IC U2_3

Reagent Disk Rotate BLACK 1 43 6 45
POW
M 2 35 14 44
STEP
BLUE 3 33 15 41
DIR
WHITE 4 23 13
M7 39
POW
M7 38
STEP
37
DIR
33
POW
30
STEP
29
DIR

MCU

MotorCB Board

1
1.10. Detectors
VDD3.3V

1 R? 300R
2
S.Arm Up/Down Upper Limit 3

PC S1

1 R? 300R
2
S.Arm Rotate H.P 3

PC S2 Invert
1 U2_1
2
Syringe UPPER LIMIT 1 R? 300R 3 MCU
2 4
3 MCU
U10
PC S3

1 R? 300R Invert
2 1 U2_2
Stirrer Arm Up/Down Upper Limit 3 2
3 MCU
PC S4 4
MCU
U10
1 R? 300R
2
Stirrer Arm Rotate H.P 3

PC S5

1 R? 300R
Rinse up/down Upper Limit 2
3

PC S6 Invert U2_3
1
2 MCU
1 R? 300R 3
Reagent Disk Rotate H.P 2 4 MCU
3
U10
PC S7

GND

MotorCB Board

2
3
1.11. Solenoid Valves, Pumps, LEDs

VDD24V

1
V 2 V1
V1
V1
Valve

1
V 2 V2
V21 Q4 DriverIC
V2
Valve Driver

BUFFER
1 SW J11 Q5 DriverIC
V 2 V3 1 1 GND
V22 3 3 Driver U24
V3 5 5 11 10
B8 GND
Valve 7 7 12 9 U4
Q6 B7 A8
9 9 DriverIC 13 8
B6 A7
11 11 14 7 MCU
B5 A6
1 13 13 Driver 15 6
B4 A5
V 2 V4 15 15 16 5 MCU
B3 A4
V23-1 to V23-3 2 2 17 4
Q7 B2 A3
P1 4 4 DriverIC 18 3
B1 A2
Valve 6 6 19 2
VDD24V GND OE A1
8 8 Driver 20 1
VCC DIR
10 10
1 12 12 VCC 74HC245 VCC
2 P1 14 14 Q9 DriverIC
P M12V
P1 16 16
P2 Driver
Pump DC3-20 DC3-20

Q3 DriverIC

1 Driver
P 2 P2
P2
DC_MOTOR
Pump

1
P3-1 to P3-4 P 2 P3 SW Board

DC_MOTOR
Pump
Main Board

4
5. Schematic Circuit Diagrams

1.12. List of Boards

No Board name Function
1 Communication Communication exchange between computer and the
Board machine’s boards.
2 Main Board Handle reaction optical signal and control reaction
table step motor
3 Motor Board Control and driver step motor’s running
4 SW Board Wire cross between Main Board and pump, valves
5 Liquid Level Detector Detect whether probe is touching liquid or knocking the
Board other Object
6 CoolCtr Board Control the temperature of reagent disk in a certain
range

The location of boards in the biochemistry analyzer is shown in the following figure:

SW Board Main Motor

Board Board

CoolCtr Board

Communicati
on Board

1
1.13. Electrical System Block Diagram

2
1.14. Communication board

This board makes communication exchange between computer and the

machine’s boards (including Main Board, and Motor Board) as a relay. It
communicates with the console computer by LAN cable and communicates
with the machine’s boards by CAN bus. It also controls the machine’s running.

1.14.1. Component Layout Diagram

Led: D4 USB Socket

Can Bus ISP button LED: D1 LED: D2 LAN

Socket socket

3
1.14.2. Description of LED functions and test Points

(1)Functions of status display LEDs

Led Name Color Normal status Meaning

D1 Green Lights D1 flicks means that MCU is working

normal
D2 Green Bright D2 is bright when the USB socket is ready
for connection with PC
D4 Red Bright Power indicator light

(2)Work when replacing board

If this board is abnormal by checking the LED’s status, you should power off the
main power of the machine before you replace this board.

1.14.3. Software update

ISP button and USB socket is used for the software update in the MCU.

The operation step about the software update is as follows:

(1) Click to install the upgrade program, then click

“Next” button until the program is installed successfully. If there are some
blanks needed to be filled with unimportant information, you can fill in
them casually. After completing the installation, you can find
in Start Menu.

4
 Picture 1
(2) Step1: Turn off the power supply and open the machine rear shell;
Step2: Put the USB cable into the USB Socket on the communication board;
Step3: Press the button which is marked with “ISP” on the communication
board;
Step4: Turn on the power, then release the ISP button.

Picture2

5
 (3) Find your device in Device Manage -Ports (COM & LPT), record the port
number. ( If your port number is greater than 100 , please right click the
mouse, select “Properties”, entry port “settings-advanced” , then modify
the COM port number to be within 100 )

Picture 3

6
(4) If the port displays that the drive has not installed, please right click the
mouse on it and choose “Update Driver Software” to install the drive.

7
 (5) Run program Demonstrator GUI, select correct port number and click
“Next”.

Picture 4
8
(6) Click “Next”. Picture 5.

Picture 5
(7) Click “Next” . Picture 6

Picture 6

9
 (8) Select Download to device, then click the quadrate button on the right of
“Download from file”.
Select hex Files(*.hex) in file type, and select ‘CBMC160.hex’. Then click
“Next”. Picture 7.

Picture 7

10
(9) When you see this dialog, that means the software have been upgraded
successfully. Picture 8.

Picture 8

(10) Check upgrade

11
 Step 1: Close the power of machine and restart it.
Step 2: Run biochemistry analyzer software on PC.
Step 3: Click “About”, and it will show the current version. You can check
the upgrade process whether it is successful. Picture 9.

Picture 9

12
1.15. Main Board

1.15.1. Functions
The Main Board is connected with the communication board by CAN bus. It
receives the commands from the communication board and completes these
functions below:

Function Name Details

Control Stepping Control and driver the reaction table rotate
motor motor
Position detect Detect the reaction table’ count/home
photocoupler and calculate the reaction table
position
Analog signal amplify Amplify the input photodiode analog signal and
and sample convert the analog signal to 16bit digital signal
Reaction Table Control Reaction Table heater to keep Reaction
temperature Control table 37℃
Lamp power supply Supply Lamp Power 6V DC
Mixing Motor control Control Mixing Motor running
Pump and Valve Control the Pumps and valves’ ON/OFF
control
Waste water and DI Get the waste water and DI sensor status
sense

13
1.15.2. Component Layout Diagram

C10 C11 C12 C13 C14

D10

D1
C20

Can
bus

D25 C15

D5

Gain

C18 C16

C17

D2

Power
input
14
1.15.3. Description of LED functions and test Points

(1) Functions of status display LEDs

Led Color Normal Meaning

Name status
D1 Red Flash When D1 is flashing, it means that the MCU
U4 is working normal
D2 Red Bright When D2 is bright, it means that the 24V
power for Mainboard is normal
D5 Red Flash When D25 is flashing, it means that the
MCU U3 is working normal
D10 Red Bright /flash When D10 is bright or flash, it means that
the Heater is working
D25 Green Bright /Flash When the reaction table is rotating, the D25
will flash, it is the Photointerrupter
S8’indicator..

(2) Value of the test points

TP Normal Value of
Details
NAME Voltage(V)
T1 0.6-2(water blank) 340 wave photodiode analog signal input
T3 0.6-2(water blank) 450 wave photodiode analog signal input
T5 0.6-2(water blank) 546 wave photodiode analog signal input
T7 0.6-2(water blank) 630 wave photodiode analog signal input
T9 reserved
T11 reserved
T8 reserved
T10 reserved
T2 0.6-2(water blank) 450 wave photodiode analog signal input
T4 0.6-2(water blank) 505 wave photodiode analog signal input
T6 0.6-2(water blank) 578 wave photodiode analog signal input
T8 0.6-2(water blank) 700wave photodiode analog signal input

A1 3±0.3(water blank) 340 wave analog signal after been amplified

A3 3±0.3(water blank) 450 wave analog signal after been amplified
A5 3±0.3(water blank) 546 wave analog signal after been amplified
A7 3±0.3(water blank) 630 wave analog signal after been amplified
A9 Reserved
A11 Reserved
A8 Reserved
A10 Reserved
15
 A2 3±0.3(water blank) 450wave analog signal after been amplified
A4 3±0.3(water blank) 505 wave analog signal after been amplified
A6 3±0.3(water blank) 578 wave analog signal after been amplified
A8 3±0.3(water blank) 700wave analog signal after been amplified

TP1 5±0.1 Analog system power supply:5V

TP2 -5±0.1 Analog system power supply:-5V
TP3 3.3±0.1 Digital system power supply:3.3V

(3) Socket information

Socket Name Connected components

C10 Reaction Table Heater
C11 Reaction Table rotation Stepper Motor
Reaction Table rotation count/home Photointerrupter
Reaction Table thermal sensor
C12 Pumps and Valves
C13 Waste water sensor
DI water sensor
C14 Mixing DC Motor
C15 Optical sensor B board
C16 Optical sensor A board
C17 Cool Fan in the back cover of the machine
C18 Lamp
CAN Bus/C7 Can bus socket connected with the communication board

(4) Work when replacing board

If this board is abnormal by checking the LED’s status, you should power off the
main power of the machine before you replace this board.

16
1.16. Motor Board

1.16.1. Functions

The Motor board is connected with the communication board by CAN bus. It
receives the commands from the communication board and completes these
functions below:

Function Name Details

Control stepping Control and driver stepping motors according the
motors commands from communication board.
Photointerrupter Detect Photointerrupter status which indicate the position
detection of the compoments
Liquid detection Get liquid detection signal status

Liquid level detection Motor board converts the input 24V supply to 5V for liquid
board power supply level detection board

17
1.16.2. Component Layout Diagram

CAN bus

C1

LED:S LED:S LED:S LED:S LED:S LED:S LED:S

C2

Power LED: C5 C4 C3
DC24V D8

18
1.16.3. Description of LED functions and test Points

(1)Functions of status display LEDs

Norma
Led
Color l Meaning
Name
status
Bright: motor board system power supply 5V is
D8 Red Bright
normal.
S1 Green Reserved
Bright: Mixing Arm is not in Vertical Home position.
S2 Green
Dark: Mixing Arm is in Vertical Home position.
Bright: Sample & Reagent Syringe is not in Home
S3 Green position.
Dark: Sample & Reagent Syringe is in Home position.
Bright: Sample & Reagent Arm is not in Horizontal
Home position.
S4 Green
Dark: Sample & Reagent Arm is in Horizontal Home
position.
S5 Green Reserved
Bright: Rinse Arm is not in Vertical Home position.
S6 Green
Dark: Rinse Arm is in Vertical Home position.
Bright: Reagent Disk is in Home position.
S7 Green
Dark: Reagent Disk is not in Home position.

(2)Value of the test points

TP NAME Normal Value Details

of Voltage(V)

TP1 3.3±0.1 Digital system and MCU power supply:3.3V

Digital system and liquid level detector board

TP2 5±0.1
power supply:5V

TP3 12±0.5 Liquid level detector board power supply:12V

TP4 -12±0.5 Liquid level detector board power supply:-12V

19
 (3)Socket information

Socket
Connected components
Name
Sample & Reagent arm up/down Stepper Motor.

Mixing arm up/down Stepper Motor.

C1
Sample & Reagent arm up/down photointerrupter.

Mixing arm up/down photointerrupter.

Sample & Reagent arm /Mixing arm horizontal Stepper Motor.

C2
Sample & Reagent arm / Mixing arm horizontal home position
photointerrupter.
Sample & Reagent Syringe Push/Pull Stepper Motor.
C3
Sample & Reagent Syringe Home position photointerrupter.

C4 Rinse Arm up/down Stepper Motor.

Rinse Arm up/down photointerrupter.

Sample & Reagent disk Stepper Motor.

C5
Sample & Reagent disk rotation Count/Home Photointerrupter

Power DC 24V Power supply

C7 Communication board

(4)Work when replacing board

If this board is abnormal by checking the LED’s status or test points, you should
power off the main power of the machine before you replace this board.

20
1.17. SW Board

1.17.1. Functions

The SW-DO board is a buffer board between Main board and Pumps, solenoid
valves.

1.17.2. Component Layout Diagram

Valve1 Valve21 Valve22 Valve23- Valve23- Valve23-

1 2 3

Pump3- Pump3- Pump3- Pump3-1 Pump Pump

4 3 2 2 1

(1) Functions of status display LEDs

Led Color Normal status Meaning

Name
D1 Red Bright Bright: Valve 1 is ON
Dark Dark: Valve 1 is OFF
D2 Red Bright Bright: Valve 2 is ON
Dark Dark: Valve 2 is OFF
D3 Red Bright Bright: Valve 3 is ON
Dark Dark: Valve 3 is OFF
D4 Red Bright Bright: Valve V23-1 to V23-3 is ON
Dark Dark: Valve V23-1 to V23-3 is OFF
D5 Red Bright Bright: Pump P3-1 to P3-4 is ON
Dark Dark: Pump P3-1 to P3-4 is OFF

21
 D6 Red Bright Bright: Pump2 is ON
Dark Dark: Pump2 is OFF
D7 Red Bright Bright: Pump1 is ON
Dark Dark: Pump1 is OFF
D8 Red Bright Reserved
Dark

(2) Work when replacing board

If this board is abnormal by checking the LED’s status, you should power off the
main power of the machine before you replace this board.

22
1.18. Liquid Level Detector Board

1.18.1. Functions

This board is an electrostatic capacitance type liquid level detector for sample
probe, and there is one collision sensor in it.

1.18.2. Component Layout Diagram

J2

Led:
DS1

JP1

Led:
DS2

Led:
DS3

J3

23
1.18.3. Description of LED functions and test Points

(1) Functions of status display LEDs

Led Normal
Color Meaning
Name status
Bright: probe is touching liquid
DS1 Green Dark
Dark: probe is not touching liquid
Bright: the board is power-on
DS2 Red Bright
Dark: the board is power-off
Bright: Sample & Reagent Arm is not in
Vertical Home position.
DS3 Red Dark
Dark: Sample & Reagent Arm is in Vertical
Home position.

(2) Socket information

Socket
Connected components
Name
J2 Analog Board for liquid detection

J3 Motor board

It is used to select collision sensor or liquid sensor signal be sent

to PC for test.
JP1
Normal work: select KN(collision sensor)
Test: select liquid sensor for test liquid sensor signal.

(3) Work when replacing board

If this board is abnormal by checking the LED’s status or test points, you should
power off the main power of the machine before you replace this board.

24
1.19. LQF Board

1.19.1. Function

This board is anti-collision optocoupler board, which function is to connect

sample probe and supply Front-end inductive circuit for sample probe liquid
level sensor.
The sample probe cannot descend when the sample probe is bounced against
an obstacle.

1.19.2. Component Layout Diagram

DS3

LQ
J2

1.19.3. Description of LED functions and test point

(1)Functions of status display LEDs

LED Color Normal Meaning

Name status
DS3 Red Dark Bright: Sample probe cannot descend
when sample probe encounters an
obstacle.
Dark: Sample probe can descend

25
 (2)Socket information

Socket Connected component

Name
J2 Liquid level detector board

LQ Sample probe

(3)Work when replacing board

If this board is abnormal by checking the LED’s status or test points, you should
power off the main power of the machine before you replace this board.

1.20. Mixing Motor optocoupler board

1.20.1. Functions
This board is the mixing motor optocoupler board.

1.20.2. Component Layout Diagram

J1

J2

1.20.3. Description of LED functions and test point

(1)Socket information
Socket Name Connected component
J1 Mixing motor control board
J2 Transfer board

(2)Work when replacing board

26
 If this board is abnormal by checking the LED’s status or test points, you should
power off the main power of the machine before you replacing this board.

1.21. CoolCtr Board

1.21.1. Functions
This board is cool control board, which function is to control the temperature of
the reagent disk within 2℃ to 8℃.

1.21.2. Component Layout Diagram

C7

LED:
D1

SIN

LED:D2
1

VOUT

VIN

27
1.21.3. Description of LED functions and test Points

(1)Functions of status display LEDs

LED Name Color Normal status Meaning

D1 Red Flash When D1 is flashing, it means that
MCU U2 is working normal.
D21 Green Bright Bright: reagent disk is cooling.

Dark Dark: reagent disk is not cooling.

(2)Socket information

Socket Name Connected component

VIN Cool control board power supply: 24 VDC

VOUT Output 24V power supply to Peltier

C7 Communication with communication board by CAN bus.

(3)Work when replacing board

If this board is abnormal by checking the LED’s status or test point, you should
power off the main power of the machine before you replace this board.

28
6. Adjustment Specifications

1.22. Reaction table adjustment

Execute Reset on maintenance screen

Adjust the three screws so that the reaction cuvette is at the center of marked
lines.

1
1.23. Reagent arm adjustment

Execute Reaction cuvette horizontal position

Adjust the parameters so that the probe is at the center of reaction cuvette

Execute Reaction cuvette vertical position

Adjust the parameters so that the top of probe is level with the reaction
cuvette.

Execute clean horizontal position

Adjust the parameters so that the probe is at the center of rinse bath
Execute clean vertical position
Adjust the parameters so that the probe descend proper height that rinse
water can reach the top of probe

2
Execute sample horizontal position and sample initial position.
Adjust those two parameters so that the probe is at the center of sample cup

Execute sample vertical position(Micro)

Adjust the parameters so that the probe descend the bottom of micro sample
cup

Execute sample vertical position(Standard)

Adjust the parameters so that the probe descend the bottom of standard
sample cup

3
4
Execute outer reagent horizontal position and outer reagent initial position
Adjust those two parameters so that the probe is at the center of outer reagent
bottle(R2)

Execute inner reagent horizontal position and inner reagent horizontal position
Adjust those two parameters so that the probe is at the center of inner reagent
bottle(R1)

Execute reagent vertical position

Adjust the parameters so that the probe descends the bottom of reagent
bottle.

5
1.24. Mixing arm adjustment

Execute reaction cuvette horizontal position

Adjust the parameters or adjustment screws so that the mixing is at the center
of reaction cuvette

Execute reaction cuvette vertical position

Adjust the parameters or adjustment screws so that the mixing descend the
bottom of reaction cuvette

Execute clean horizontal position

Adjust the parameters so that the mixing is at the center of rinse bath

Execute clean vertical position

Adjust the parameters so that the mixing descend proper height that rinse
water can reach the mixing

6
1.25. Clean arm adjustment

Execute reaction cuvette vertical position

Adjust the parameters or adjustment screws so that the mixing descend the
bottom of reaction cuvette

7
1.26. Lamp position adjustment

Adjust those two screws so that the center of optical fiber is covered by light
spot

8
1.27. Water Blank (AD) value adjustment

When the Lamp is at the end of its life, you need to replace the Lamp.
Because of the difference of Lamp’ luminance, you need to adjust the water
blank value to be a normal range (45000-55000) by rotate the potentiometers.

You can adjust the water blank value by the following steps:

1) Remove the cuvette segment.1(1-11) on the reaction table;

9
 2) Open the “Test.exe” tool in the root directory of the analyzer
software(default: D:/BioApp/App), click AD button to go to AD window, then
click start button, the screen display the AD value of the eight wavelength.

AD button

3) Open the back cover of the machine, and you will see the Main Board of the
machine.
10
 Gain
button

Offset
adjust

4) Press Gain button 3s, and loosen, the AD value will be automatically
adjusted to between 45000 and 55000.

11
 AD value

5) Turn off the analyzer power switch, and power on the analyzer, repeat
steps 2-4 to check whether the AD value is within a normal range.

You can adjust the offset value by following steps:

12
1) Place the opaque paper in the No.1 reaction cuvette to block the light
through the cuvette.

2) Rotate the potentiometer (offset adjust) on the main board to adjust the
offset value until the offset value of the eight wavelength is between 10
and 30.

13
Offset
value

3) Click clear button, observe continuously the signal value of 20 points, the
Diff value is not exceed 12.

14
4) Remove the opaque paper, observe the AD value whether is between
45000 and 55000.
5) Install the cuvette segment.1(1-11) on the reaction table.

6) Click start button in the software, observe continuously the signal value of
100 points, the Diff value is not exceed 50.

15
 7) Click “Period” button, the Diff value is not exceed 200 when static and
dynamic switch.

16
8) Click “Clear” button, observe continuously the signal value of 20 points, the
Diff value is not exceed 90.

17
7. Instrument malfunction and alarm handling
1.28. Details of Alarm

When the machine is abnormal or the test is abnormal, the software in console
computer will alarm in the ALARM BUTTON in the right side of the software. The
detail is described in the table below.

Alarm level Detail

Warning Abs. Check Error

Reagent runs out

Sample runs out

Diluent runs out

Test time out

Mean. Check Error

Lamp error

S.Stop Insufficient water

Waste liquid overflow

Stop The sample probe collisions

Reaction disk's optocoupler breaks down

The machine runs timeout

Instruction timeout

Communication failure

Instruction execution failure

The add sample arm vertical motor's optocoupler broke down

The add sample arm horizontal motor's optocoupler broke down

The mix arm vertical motor's optocoupler broke down

The mix arm horizontal motor's optocoupler broke down

The clean arm vertical motor's optocoupler broke down

The reagent sample disk motor's optocoupler broke down

The injector's optocoupler broke down

1
 Communication failed! The main board has broken down

Communication failed! The temperature control board has broken

down
Communication failed! The motor control board A has broken down

Communication failed! The motor control board B has broken down

2
1.29. Troubleshooting

No. Alarm Alarm Cause Handling

level content/Malfunction

1 Slight XX sample runs out The sample is insufficient  Stop adding sample
 Add the sample
 Refresh sample status
 Continue to work
2 Slight XX reagent runs out The reagent is insufficient  Adding reagent
 Refresh reagent status
3 Medium XX cup absorbance error The deionized water is  Add deionized water
insufficient and there are  Perfusion operation
many bubbles in the
 Execute the reaction cell blank for 2
pipe.
times
 Refer 7.2.1 chapter for details.
The reaction cuvette is  Replace the damaged reaction
cracked and the water is cuvette
overflowing  Clean reaction table
 Clean the outer wall of all reaction
cuvettes.
 Refer 7.2.1 chapter for details.
The cleaning arm does  Check the pump working condition
not absorb the water and  Check whether the cleaning arm
the water will overflow horizontal position deviates from
the center of the reaction cuvette
 Check whether the washing probe is
blocked
 Refer 7.2.1 chapter for details.

3
 4 Medium Lamp error Lamp life expiration  Check whether there is light source
in the reaction table
 Replacement lamp
5 Medium When the reagent 1)Probe plugging  Use the needle to dredge the
probe/sample probe reagent probe / sample probe
rises from the wash
bath, the probe hangs a  Open and clean the solenoid valve
drop of water and spits 2)There are foreign  Refer 7.2.2 chapter for details.
objects inside the
corresponding solenoid
valve
6 Serious liquid level detection 1)The instrument has no  Instrument ground
exception protection ground
2)The conductivity of
deionized water is not  Replace the standard deionized
up to standard water
3)Bad contact of line
cable
 Replace the power cable of the
liquid level detector board
 Refer 7.2.3 chapter for details.
7 Serious Communication failed 1)The main power supply
is not opened
2)The plug is loose
3)When the instrument is
running, it is subjected  Refer 7.2.4 chapter for details.
to the strong
electromagnetic
interference of the
centrifuge
8 Serious Reaction disk’s 1)The reaction table  Refer 7.2.5 chapter for details.
optocoupler error motor is abnormal
2)There are foreign
objects in the reaction
table

4
 3)The motor cable or the
optical coupling cable
is affected by external
forces lead to bad
contact (to be bitten by
a mouse)
9 Serious Reagent disk’s optical 1)The reagent disk motor
coupling error is abnormal
2)There are foreign
objects in the reagent
disk
3)The motor cable or the  Refer 7.2.6 chapter for details.
optical coupling cable
is affected by external
forces lead to bad
contact (to be bitten by
a mouse)
10 Serious Sample arm 1)Abnormal motor
horizontal/vertical motor operation
optical coupling error 2)The motor cable or the
optical coupling cable
is affected by external
forces lead to bad
contact (to be bitten by
a mouse
11 Medium Individual test results 1)The result of the  View reaction curve
are always low or high calibration is not in  Reevaluate the project or modify
conformity with the the calibration parameters
requirements
2)The performance  Change the reagent
failure of the reagent

5
 12 Serious A large number of test 1)Instrument failure  Check the instrument alarm record
result exceptions 2)Batch failure of reagent  View reaction curve
 View the cup blank record

6
1.29.1. XX cup absorbance error
1, Details of malfunction
The software alarm XX cup absorbance error when system is testing.

2, Troubleshooting
Cause1: The deionized water is insufficient and there are many bubbles in
the pipe.
Handling: 1, Add the deionized water into the deionized water tank.
2, Perform the Air Purge operation on maintenance screen.
3, Perform the Cell Blank operation for 2 times on maintenance screen.

Air Purge Cell Blank

Cause2: The reaction cuvette is cracked and the water is overflowing.

Handling: 1, Replace the damage reaction cuvette in reaction table;
2, Clean the reaction table;
3, Clean the outer wall of all reaction cuvette.

Cause3: The cleaning arm does not absorb the water and the water will
overflow.
Handling: 1, Check the pump working condition.

6
 2, Check whether the cleaning arm horizontal position deviates from the
center of the reaction cuvette.
3, Check whether washing probe is blocked.

Remarks: If alarm recurs, call Technical Support.

1.29.2. Reagent/Sample probe drop water

1, Details of malfunction
When the reagent probe/sample probe rises from the wash bath, the probe
hangs a drop of water and spits

2, Troubleshooting
Cause1: The reagent needle/sample needle plugging.
Handling: Use the needle to dredge the reagent needle/sample needle.

Cause2: There are foreign objects inside the corresponding solenoid valve.
Handling: Open and clean the corresponding solenoid valve.

Remarks: If alarm recurs, call Technical Support.

1.29.3. Liquid level detection exception

1, Details of malfunction
The software alarm Liquid level detection exception when system is testing.

2, Troubleshooting
Cause1: The instrument has no protection ground.
Handling: Instrument shell connects to ground.

Cause2: The conductivity of deionized water is not up to standard.

Handling: Replace the standard deionized water.

Cause3: Bad connect of line cable.

Handling: Replace the power cable of liquid level board.

Remarks: If alarm recurs, call Technical Support.

1.29.4. Communication failed

7
 1, Details of malfunction
The software alarm communication failed and the instrument stops working.

2, Troubleshooting
Cause1: The main power supply is not opened.
Handling: Open the main power supply of the analyzer.

Cause2: The net cable of plug is loose.

Handling: Reconnect and fix the net cable.

Cause3: When the instrument is running, it is subjected to the strong

electromagnetic interference of the centrifuge.
Handling: Replug the net port on operation computer side and restart the
operation software.

Remarks: If alarm recurs, call Technical Support.

1.29.5. Reaction disk’s optocoupler error

1, Details of malfunction
The software alarm reaction disk’s optocoupler error when the instrument is
running.

2, Troubleshooting
Cause1: The reaction table’s motor is abnormal.
Handling: Replace the reaction table’s motors.

Cause2: There are foreign objects in the reaction table.

Handling: Open and clean the reaction table.

Cause3: The motor cable or the optical cable is affected by external forces
lead to bad contact (to be bitten by mouse)
Handling: Replace the motor cable or the optical cable.

Remarks: If alarm recurs, call Technical Support.

1.29.6. Reagent disk’s optical coupling error

8
 1, Details of malfunction
The software alarm reagent disk’s optical coupling error when the instrument is
running.

2, Troubleshooting
Cause1: The reagent disk motor is abnormal.
Handling: Replace the reagent disk motor.

Cause2: There are foreign objects on the reagent disk.

Handling: Open and clean the reagent disk.
Cause3: The motor cable or the optical cable is affected by external forces
lead to bad contact (to be bitten by mouse)
Handling: Replace the motor cable or the optical cable.

Remarks: If alarm recurs, call Technical Support.

1.29.7. No water from the sample&reagent probe

1, Details of malfunction
The sample&reagent probe does not get out of water when the analyzer
washes the sample&reagent probe.

2, Troubleshooting
Cause1: The corresponding solenoid valve is plugging.
Handling: Open and clean the corresponding solenoid valve.

Cause2: Feed pump failure.

Handling: Replace the corresponding solenoid valve.

Cause3: Main board has broken down.

Handling: Replace the Main board.

1.29.8. Reaction cuvette overflow

1, Details of malfunction

9
 The reaction cuvette is overflow when the washing probe on the washing arm
is washing reaction cuvettes.

2, Troubleshooting
Cause1: The corresponding solenoid valve plugging.
Handling: Replace the corresponding solenoid valve.

Cause2: Waste water pump failure.

Handling: Replace waste water pump.

Cause3: Main board has broken down.

Handling: Replace the Main board.

10
8. Maintenance
1. The daily maintenance
1) Clean reaction cuvette and waste liquid line：
Select "maintenance" in the main interface. Perform the Wash Cells (all)
procedures, Clean all the reaction cuvettes and waste water lines.

2) Cleaning instrument panel：

Keep the surface of the instrument clean and beautiful.

2. Weekly maintenance
1) Clean reaction cuvette：
Remove the bracket and the reaction cuvette with the bracket from the
instrument, Soak for 12 hours in 2% of antibacterial and unphosphating
solution. Then remove and wash with pure water, Put back the instrument after
drying.

2) Clean sample probe, reagent probe, mixing arm:

Use a concentration of 75% alcohol to wipe down the tips and stirs, Clean the
tips and mixing.

3) Dredging the Sample probe and reagent probe:

Using attachments box Φ 0.3 needle (for sample)/ Φ 0.4 needle (for reagent)
,Dredge from down to up.

3. Monthly maintenance
1) Wash out the deionized water bucket：
Check the inside of the deionized water bucket. If any foreign body or scale is
found, clean it with pure water.

2) Cleaning sample disk and the reagent disk：

Power off, Screw loose and remove the fixed screw from the sample disk k and
the reagent disk, Remove reagent disk and sample disk. Dust and liquid
residue in cleaning disk and silo.

4. Check work before the daily test

1) Check that the ionized water is adequate.

2) Check for smooth diskharge of waste liquid. It is forbidden to insert the
waste liquid tube below the liquid level.
3) Perform the perfusion operation. Ensure that the instrument piping is full of
deionized water.

1
 5. The maintenance work after the daily test
1) Perform the "wash cells" operation, Clean the pipe and reaction cuvettes.

6. Replace the consumables

1) Instrument alarm "lamp error", And when you make sure that the lamp
doesn't light in the reaction table, Inform the engineer to replace the lamp.
2) The reaction cuvette should be changed once a year.
3) The sealing ring on the syringe should be replaced every year.

7. Common alarm
1) Cup blank error, Error in absorbance of XX cup.
Cause: It could be the lack of ionized water, or the expiration of the reaction
cuvette, Cracking, or the diskharge of waste liquid is not smooth.
The solution: Check the adequacy of deionized water, Whether the diskharge of
waste liquid is unobstructed. Does the reaction cuvette have spilled water.

2) Reagent depletion
The solution: Adding reagents, And refresh the state of the reagents in the
software.

3) Sample depletion：
The solution: Add samples, And refresh the state of the sample in the software.

2
修订记录

修订日期 修订后版本 修订前版本 修改内容

2019.10.2 V1.1 V1.0 1.排版更改；2.专业名词更换；
2