PSV COLLEGE OF PHARMACEUTICAL SCIENCE & RESEAECH
KRISHNAGIRI - 635108
FORMULATION AND EVALUATION OF CURCUMIN-LOADED
CHITOSAN GEL
PRESENTED by
DEVIKA.M GAYATHRI
HEESHAN RAJU.R KHAVIYA.M
UNDER THE GUIDANCE OF
Mrs. a. chandra., M.PHARM.,
DEPARTMENT OF PHARMAceutics
INTRODUCTION
This presentation summarizes the formulation and evaluation of a
curcumin-loaded chitosan topical gel designed for wound
healing. Curcumin's anti-inflammatory, antioxidant, and
antimicrobial properties are combined with chitosan's
biocompatibility to create a promising therapeutic agent. The
study explores the gel's characteristics, release kinetics, and
potential efficacy for promoting tissue regeneration and
preventing infections.
REVIEW OF LITERATURE
Sideek et al. (2023) reviewed various curcumin-loaded delivery systems for wound healing, including
nanoparticles, hydrogels, and liposomes, to enhance curcumin’s bioavailability and therapeutic efficacy. The study
emphasized chitosan-based formulations for their antimicrobial and regenerative properties. Challenges in
formulation stability and clinical translation were also discussed.
Gupta et al. (2023) investigated curcumin-loaded chitosan hydrogel for diabetic wound healing using FTIR and
GC-MS analysis. FTIR confirmed successful curcumin incorporation, while GC-MS identified its chemical
stability. The study highlighted the hydrogel’s antimicrobial efficacy and potential for enhanced wound healing.
Edula et al. (2023) formulated and evaluated a polyherbal gel for wound healing, incorporating multiple medicinal
plant extracts. The study assessed the gel’s physicochemical properties, antimicrobial activity, and wound-healing
potential. Results indicated enhanced healing effects, supporting its use as a natural therapeutic option.
AIM & OBJECTIVE: Crafting a Novel
Wound Healing Agent
This study seeks to harness the synergistic properties of curcumin and
and chitosan to create a topical gel that accelerates wound closure and
and reduces inflammation. Our aim is to formulate and evaluate a curcumin-
curcumin-loaded chitosan topical gel for effective wound healing. The
The primary objective is to develop a topical gel with enhanced wound
wound healing properties.
Formulation and Development Physicochemical Characterization
Creating a stable and effective gel Assessing the gel's properties like pH
gel formulation like pH and viscosity
In-Vitro Evaluation
Testing drug release and biocompatibility
Curcumin: A Bioactive Compound
Anti-inflammatory
1
2 Anti-oxidant
3 Anti-microbial
Curcumin, derived from turmeric, is known for its medicinal benefits. It exhibits antioxidant, anti-inflammatory, antiviral,
antibacterial, antifungal, and cancer chemopreventive properties. Recent studies suggest curcumin can hinder carcinogenesis
and angiogenesis. However, its poor water solubility limits its biomedical applications, making delivery mechanisms crucial for
enhancing its pharmacological efficacy.
Chitosan: A Versatile Biopolymer
Antimicrobial Activity
Biodegradability
Heavy Metal Adsorption
Chitosan, a natural biopolymer from chitin, offers biocompatibility,
biodegradability, and biological activities. It is derived from crustacean shells
and has diverse applications in pharmaceuticals, agriculture, food, and
biomedicine. Chitosan’s antimicrobial activity, biodegradability, and heavy metal
adsorption capabilities make it an eco-friendly and sustainable option in various
fields.
Wound Healing Process & Curcumin's Role
1 Hemostasis
Platelets clog to stop blood loss.
2 Inflammation
Leukocytes remove germs.
3 Proliferation
Tissue regeneration by contraction.
4 Remodeling
ECM remodeling to restore structure.
Wound healing, involving hemostasis, inflammation, proliferation, and remodeling, requires a coordinated
process for tissue restoration. Curcumin's properties enhance wound healing by promoting granulation
tissue formation, new blood vessel development, and re-epithelialization. Efficient drug delivery systems
are essential to enhance curcumin's therapeutic effectiveness and overcome its solubility and stability
issues.
Gels: Structure and Properties
2 Hydrophilic Colloid
Particles can be spherical or
macromolecules.
Semi-Rigid System
Movement is limited by interconnected 1
network of particles.
Attractive Forces
Particles held together by valencies or
weaker forces like hydrogen bonds.
3
Gels are semi-rigid systems where particle movement is restricted by an interwoven network. This structure results from
interconnected particles of a gelling agent, defining the network configuration and gel attributes. The strength of the attractive
forces holding particles together can vary from strong primary valencies to weaker hydrogen bonds, influencing gel properties.
MATERIALS & METHODS: The Building Blocks of Our
Formulation
Our formulation relies on carefully selected materials known for their biocompatibility and therapeutic potential. This section
outlines the key ingredients and methods used in the preparation and evaluation of our curcumin-loaded chitosan topical gel.
Key Ingredients Methods Used
• Curcumin (Active ingredient) • Pre-formulation studies
• Chitosan (Biopolymer) • Gel preparation
• Carboxymethyl Cellulose (Gelling agent) • Evaluation tests (pH, viscosity, drug release, stability)
Preparation of Curcumin-Loaded Chitosan Gel: A Step-by-Step Guide
The preparation of our curcumin-loaded chitosan gel involves a meticulous process to ensure uniform drug distribution and optimal gel
properties. Each step is critical to the final product's efficacy and stability. Precise control over variables like temperature and mixing speed
guarantees the consistency and reproducibility of our formulation.
Chitosan Solution
Dissolve chitosan in acetic acid
Gel Formation
Add gelling agents and preservatives
Curcumin Solution
Dissolve curcumin in ethanol
Homogenization
Ensure uniformity
Mixing
Combine the two solutions
Evaluation Parameters: Assessing Gel Quality and Performance
To ensure the quality and efficacy of our curcumin-loaded chitosan gel, we subjected it to a series of rigorous evaluation tests. These
parameters provide critical insights into the gel's physicochemical properties, drug release kinetics, and stability under different storage
conditions.
1 Homogeneity and Clarity
2 pH Measurement
3 Viscosity Study
4 In-Vitro Diffusion
Results and Discussion: Unveiling
the Potential of Our Formulation
The results of our evaluation tests provide compelling evidence for the potential of
curcumin-loaded chitosan gel as an effective wound healing agent. Our findings
highlight the optimal performance of the F3 formulation, suggesting a promising
avenue for further investigation and clinical translation.
Neutral pH
7.5 ± 0.5 ensures skin compatibility
Optimal Release
F3 released 79.7% in 120 mins
Spreadability
Good and stable formulations
Conclusion: A Promising Avenue for Wound Treatment
Our research demonstrates the potential of curcumin-loaded chitosan gel as a promising alternative for conventional wound treatments.
The optimal performance of the F3 formulation highlights the importance of formulation optimization in achieving desired therapeutic
outcomes.
Optimal Performance
1
2 Effective Healing
3 Chitosan Gel
