YPD Broth (YEPD Broth) M1363
YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes.
Composition**
Ingredients Gms / Litre
Peptic digest of animal tissue 20.000
Yeast extract 10.000
Dextrose 20.000
Final pH ( at 25°C) 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving
at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
YPD Broth (YEPD Broth) is recommended by Sherman (1) for the growth of Saccharomyces cerevisiae for molecular
biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition,
they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using
Yeast Extract Peptone- Dextrose (YPD) medium for cultivating
S. cerevisiae and other yeasts (2). Yeasts grow well on a minimal medium containing only dextrose and salts. The addition
of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells
divide every 90 minutes (2).
The medium composition aids in growth of Saccharomyces . Peptic digest of animal tissue provides nitrogenous nutrients.
Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy
source to support growth of S. cerevisiae
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes.
Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH : 6.5±0.2
pH
6.30-6.70
Cultural Response
M1363: Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
Organism Inoculum Growth
(CFU)
*Aspergillus brasiliensis 50-100 good-luxuriant
ATCC 16404
Candida albicans ATCC 50-100 good-luxuriant
10231
Saccharomyces cerevisiae 50-100 good-luxuriant
ATCC 9763
*Key: Formerly known as Aspergillus niger
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
1. Sherman F., Meths. Enzymol. 194, 3 (1991).
2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. and Struhl K., 1994, Current protocols
in molecular biology, Current Protocols, Brooklyn, N.Y.
Revision : 02 / 2015
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