Y - JOUR

T
AU - Ghanati, F.
AU - Bakhtiarian, S.
TI - Effect of methyl jasmonate and silver nanoparticles on production of secondary metabolites
by Calendula Officinalis L (Asteraceae)
PY - 2014
T2 - Tropical Journal of Pharmaceutical Research
VL - 13
IS - 11
SP - 1783
EP - 1789
DO - 10.4314/tjpr.v13i11.2
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84912140599&doi=10.4314%2ftjpr.v13i11
.2&partnerID=40&md5=243fc8b78a06e532c4b247f98bb8c68f
AD - Department of Plant Biology, Tarbiat Modares University, Tehran, Iran
AB - Conclusion: Treatment of C. officinalis L. with SNPs and MeJA seems to be a simple and
cost-effective method of improving the medicinal properties of this plant.; Purpose: To
investigate the effects of two elicitors, methyl jasmonat (MeJA) and silver nanoparticles (SNPs),
on the production of secondary metabolite by Calendula officinalis L (marigold).; Methods: For
the extract of the aerial part of the plant, membrane lipid peroxidation rate and 2,
2ˈdiphenylpicrylhydrazyl (DPPH) radical scavenging activity were assessed along with
totalanthocyanin, flavonoid, chlorophyll, carotenoids and saponin content. The effect of C.
officinalis L extract with and without SNPs and MeJA on HeLa cell viability was also evaluated
by methylthiazol tetrazolium (MTT) assay.; Results: The presence of SNPs and MeJA in
marigold extract increased membrane lipid peroxidation but decreased DPPH radical
scavenging activity. Anthocyanin and flavonoid content also decreased in all the treatments
investigated. Exposure to SNPs decreased chlorophyll and carotenoid content in the plant by 30
- 50%, while MeJA increased them. In comparison with the control group, treatment with 0.4 mM
SNPs and 100 µM MeJA increased saponin content in the plants by up to 177%. Exposure of
HeLa cells to the extracts of marigold significantly reduced their viability and this reduction was
more pronounced when the plants were treated with MeJA and SNPs. © Pharmacotherapy
Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.
KW - Calendula officinalis
KW - HeLa cells
KW - Membrane lipid peroxidation
KW - Methyl jasmonate
KW - Radical scavenging
KW - Secondary metabolites
KW - Silver nanoparticles
KW - anthocyanin
KW - Calendula officinalis extract
KW - carotenoid
KW - chlorophyll
KW - flavonoid
KW - jasmonic acid methyl ester
KW - plant extract
KW - saponin derivative
KW - silver nanoparticle
KW - unclassified drug
KW - analytical parameters
KW - Article
KW - Calendula officinalis
KW - cell viability
KW - controlled study
KW - cytotoxicity
KW - DPPH radical scavenging assay
KW - drug effect
KW - human
KW - human cell
KW - lipid peroxidation
KW - lipid peroxidation assay
KW - metabolite
KW - MTT assay
KW - nonhuman
KW - ultraviolet spectrophotometry
PB - University of Benin
SN - 15965996 (ISSN)
LA - English
J2 - Trop. J. Pharm. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 51
ER -

TY - JOUR
AU - Banakar, S.M.
AU - Veerapur, V.P.
AU - Thippeswamy, B.S.
AU - Jagadeesh, N.V.
AU - Gavimath, C.C.
AU - Alshehri, Z.S.
TI - Protective Effect of Calendula officinalis (L.) Flower Extract in Acetic Acid–Induced
Ulcerative Colitis in Rats
PY - 2016
T2 - Journal of Herbs, Spices and Medicinal Plants
VL - 22
IS - 3
SP - 225
EP - 237
DO - 10.1080/10496475.2016.1190952
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979555840&doi=10.1080%2f10496475
.2016.1190952&partnerID=40&md5=8dc5cd9b4dfc68f45d1519af90eec439
AD - Soniya Education Trust’s College of Pharmacy, Karnataka, India
AD - Sree Siddaganga College of Pharmacy, Karnataka, India
AD - College of Pharmacy, Al-Dawadmi, Shaqra University, Ministry of Higher Education, Saudi
Arabia
AD - K. L. E. Society’s College of Engineering and Technology, Karnataka, India
AB - ABSTRACT: Rats were pretreated with graded doses of Calendula officinalis flower extract
(COE) for 7 consecutive days and then induced colitis by intrarectal acetic acid. The treatment
with COE was continued up to 10 d. Pretreatment with COE decreased clinical activity and
macroscopic scores and reduced the colonic myeloperoxidase activity, lipid peroxides, and
serum lactate dehydrogenase, and increased the reduced glutathione. These findings were
confirmed by the histopathological studies that followed. © 2016 Taylor & Francis.
KW - Compositae
KW - inflammation
KW - irritable bowel diseases
KW - myeloperoxidase
KW - Asteraceae
KW - Calendula officinalis
KW - Rattus
KW - acetic acid
KW - Calendula extract
KW - Calendula officinalis extract
KW - flower extract
KW - glutathione
KW - lactate dehydrogenase
KW - lipid peroxide
KW - myeloperoxidase
KW - salazosulfapyridine
KW - unclassified drug
KW - acetic acid
KW - bacterial disease
KW - biological control
KW - disease control
KW - disease treatment
KW - enzyme
KW - enzyme activity
KW - herb
KW - histopathology
KW - plant extract
KW - rodent
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - biochemical analysis
KW - Calendula officinalis
KW - controlled study
KW - drug effect
KW - enzyme activity
KW - female
KW - histopathology
KW - lactate dehydrogenase blood level
KW - lipid peroxidation
KW - male
KW - nonhuman
KW - rat
KW - ulcerative colitis
PB - Taylor and Francis Inc.
SN - 10496475 (ISSN)
LA - English
J2 - J. Herbs Spices Med. Plants
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: B.S. Thippeswamy;
Department of Biomedical Science, College of Pharmacy, Shaqra University, Ministry of Higher
Education, Al-Dawadmi, Saudi Arabia; email: t_swamy@hotmail.com; CODEN: JHEPE
ER -

TY - JOUR
AU - Hormozi, M.
AU - Gholami, M.
AU - Babaniazi, A.
AU - Gharravi, A.M.
TI - Calendula officinalis stimulate proliferation of mouse embryonic fibroblasts via expression
of growth factors TGFβ1 and bFGF
PY - 2019
T2 - Inflammation and Regeneration
VL - 39
IS - 1
C7 - 7
DO - 10.1186/s41232-019-0097-x
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084933928&doi=10.1186%2fs41232-0
19-0097-x&partnerID=40&md5=80ccb69977694a96d5d3be293893acc5
AD - Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences,
Khorramabad, Iran
AD - Department of Biochemistry, Lorestan University of Medical Science, Khorramabad, Iran
AD - Department of Anatomical Sciences, Kermanshah University of Medical Sciences,
Kermanshah, Iran
AD - Student Research Committee, Lorestan University of Medical Sciences, Khorramabad,
Iran
AD - Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical
Sciences, Shahroud, Iran
AB - Background: TGF-β has an important role in the process of wound healing and scar
formation. The aim of this study is to determine the effects of ethanolic and methanolic extracts
of Calendula officinalis on the expression of TGFβ1 and bFGF in the mouse embryonic
fibroblast cells (MEFs). Methods: Calendula officinalis extract was purchased and different
substances defined with gas chromatography and mass spectrometry. MEFs were prepared and
after incubating for 15 min, cell viability analyzed. TGF β 1 and bFGF gene expression was
evaluated by real-time PCR. TGFβ1 and bFGF protein expression analyzed by ELISA. The
statistical analysis of data was done by using SPSS software. Differences were considered
significant at (P < 0.05). Results: The results of the MTT test showed that the concentrations of
5 μg/ml and10 μg/ml were more suitable for cell proliferation. There was an increase in TGF β 1
gene expression in the MEFs. Expression of TGF β 1 gene remains the same after 24 h. Gene
expression of bFGF showed a similar pattern with TGF β 1 expression for both solvents.
Analysis of TGFβ1 protein expression showed an increase in TGFβ1 gene expression in the
MEFs. Protein expression of bFGF in the MEFs increased at different concentrations at 12 and
24 h after treatment (P < 0.05 and P < 0.01 respectively). Conclusion: Calendula officinalis
stimulates proliferation of MEFs. Calendula via increased expression of growth factors (TGFβ1
and bFGF) at the first 12 h and a decrease of these factors at 24 h after treatment may
ameliorate function of the MEFs in the during wound healing. © 2019 The Author(s).
KW - bFGF
KW - Calendula officinalis
KW - Mouse embryonic fibroblasts
KW - TGFβ1
KW - Calendula extract
KW - Calendula officinalis extract
KW - fibroblast growth factor 2
KW - transforming growth factor beta1
KW - unclassified drug
KW - Article
KW - Calendula officinalis
KW - cell proliferation
KW - cell viability
KW - controlled study
KW - drug effect
KW - embryo
KW - enzyme linked immunosorbent assay
KW - fibroblast
KW - gene expression
KW - incubation time
KW - mass fragmentography
KW - mouse
KW - mouse embryo
KW - MTT assay
KW - nonhuman
KW - priority journal
KW - protein expression
KW - real time polymerase chain reaction
KW - treatment duration
PB - BioMed Central Ltd.
SN - 18808190 (ISSN)
LA - English
J2 - Inflamm. Regen.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 4; Correspondence Address: A.M. Gharravi;
Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences,
Shahroud, Iran; email: annehgh@yahoo.com
ER -

TY - JOUR
AU - Fierascu, I.
AU - Bunghez, I.-R.
AU - Fierascu, R.
AU - Ion, R.-M.
AU - Dinu-Pîrvu, C.E.
AU - Nuţǎ, D.
TI - Characterization and antioxidant activity of phytosynthesised silver nanoparticles using
Calendula officinalis extract
PY - 2013
T2 - Farmacia
VL - 62
IS - 1
SP - 129
EP - 136
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893656650&partnerID=40&md5=2523
5fbc4c119a48518e455fd9217a17
AD - National R and D Institute for Chemistry and Petrochemistry - ICECHIM, 060021,
Bucharest, 202 Splaiul Independentei Street, Romania
AD - University of Medicine and Pharmacy,'Carol Davila', 020956 Bucharest, 6 Traian Vuia
Street, Romania
AB - The present paper describes an eco-friendly bottom-up approach to design metallic
nanoparticles with biomedical potential. The aim of the study was to characterize in terms of
antioxidant activity the silver nanoparticles obtained using Calendula officinalis petals extract.
First of all, a visual inspection confirmed the formation of silver nanostructures by colour
changing of the sample. The bioreduction of silver ions was analyzed by modern analytical
techniques (UV-VIS, FTIR (Fourier transformed IR spectroscopy), XRF (energy-dispersive X-ray
fluorescence) spectroscopy and SEM (Scanning Electron Microscopy)). Antioxidant activity of
the silver nanostructures was evaluated using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)
method. The antioxidant activity of the phytosynthesised nanoparticles proved to be superior to
the results of the extract.
KW - Antioxidant activity
KW - Calendula officinalis
KW - Green synthesis
KW - Silver nanoparticles
KW - Calendula officinalis extacr
KW - hydroquinone
KW - plant extract
KW - silver nanoparticle
KW - unclassified drug
KW - antioxidant activity
KW - article
KW - color
KW - controlled study
KW - DPPH radical scavenging assay
KW - flower
KW - infrared spectroscopy
KW - marigold
KW - nonhuman
KW - scanning electron microscopy
KW - storage
KW - X ray fluorescence
PB - Romanian Society for Pharmaceutical Sciences
SN - 00148237 (ISSN)
LA - English
J2 - Farmacia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 26; Correspondence Address: I.-R. Bunghez;
National R and D Institute for Chemistry and Petrochemistry - ICECHIM, 060021, Bucharest,
202 Splaiul Independentei Street, Romania; email: raluca_bunghez@yahoo.com; CODEN:
FRMBA
ER -

TY - JOUR
AU - Kharat, Z.
AU - Amiri Goushki, M.
AU - Sarvian, N.
AU - Asad, S.
AU - Dehghan, M.M.
AU - Kabiri, M.
TI - Chitosan/PEO nanofibers containing Calendula officinalis extract: Preparation,
characterization, in vitro and in vivo evaluation for wound healing applications
PY - 2021
T2 - International Journal of Pharmaceutics
VL - 609
C7 - 121132
DO - 10.1016/j.ijpharm.2021.121132
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116324812&doi=10.1016%2fj.ijpharm.
2021.121132&partnerID=40&md5=b33841a972027ebe567533fea2de847d
AD - Department of Biotechnology, College of Science, University of Tehran, Tehran,
14155-6455, Iran
AD - Department of Life Science Engineering, Faculty of New Sciences & Technologies,
University of Tehran, Tehran, 14395-1561, Iran
AD - Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of
Tehran, Tehran, 1417466191, Iran
AB - Wound healing is a complex pathophysiological process, highlighting the importance of
effective and thorough wound care along with the prevention of wound infection, a major barrier
that can slow down or even disrupt the healing process. To date, there are plenty of herbal
plants well known and historically supernatural, showing profound wound healing effects.
Application of such herbal extracts/ingredients in electrospun nanofiber platforms has shown
promising outcomes in improving wound healing process. Based on these facts, we loaded
Calendula officinalis extract (CO) in chitosan/polyethylene oxide scaffolds (CS/PEO) by
electrospinning. Using SEM, morphology of electrospun scaffolds showed a narrow range of
fiber diameter, around 143–252 nm, with uniform and bead-free appearance. FT-IR
spectroscopy confirmed the presence of CO extract in nanofibrous scaffolds. Of importance,
incorporation of CO extract improved mechanical properties of CS/PEO nanofibers. A 1602 cP
reduction in viscosity and a 0.892 ms/cm increase in the conductivity of the solution was
observed after addition of the CO extract. CO extract showed strong antibacterial properties
with 96% and 94% reduction in Gram positive and Gram negative bacteria, respectively. In vitro
studies with fibroblast cells confirmed enhanced proliferation, growth and attachment of the
cells. The in vivo and histological analysis of rat wounds, revealed excellent wound healing
ability of CS/PEO/CO dressings (87.5 % wound closure after 14 days) via improving collagen
synthesis, re-epithelization and remodeling of the tissue. In sum, our findings show that
CS/PEO/CO scaffolds can be used as a promising dressing for the treatment of skin wounds. ©
2021 Elsevier B.V.
KW - Calendula officinalis extract
KW - Chitosan
KW - Electrospun nanofiber
KW - PEO
KW - Wound dress
KW - Wound healing
KW - Animals
KW - Anti-Bacterial Agents
KW - Calendula
KW - Chitosan
KW - Gram-Negative Bacteria
KW - Gram-Positive Bacteria
KW - Nanofibers
KW - Plant Extracts
KW - Rats
KW - Spectroscopy, Fourier Transform Infrared
KW - Wound Healing
KW - Calendula extract
KW - chitosan
KW - enrofloxacin
KW - glutaraldehyde
KW - ketamine
KW - macrogol
KW - molecular scaffold
KW - tramadol
KW - xylazine
KW - antiinfective agent
KW - nanofiber
KW - plant extract
KW - absorption
KW - adult
KW - angiogenesis
KW - animal cell
KW - animal experiment
KW - animal tissue
KW - antibacterial activity
KW - Article
KW - bacterial count
KW - bacterium colony
KW - biocompatibility
KW - biodegradation
KW - biomechanics
KW - Calendula officinalis
KW - cell adhesion
KW - cell growth
KW - cell maturation
KW - cell proliferation
KW - collagen synthesis
KW - conductance
KW - contact angle
KW - content uniformity
KW - controlled study
KW - cross linking
KW - dissolution rate constant
KW - drug release
KW - electrospinning
KW - epithelization
KW - Escherichia coli
KW - fibroblast
KW - Fourier transform infrared spectroscopy
KW - granulation tissue
KW - growth rate
KW - histopathology
KW - hydrogen bond
KW - hydrophilicity
KW - immunohistochemistry
KW - in vitro study
KW - in vivo study
KW - inflammation
KW - inflammatory infiltrate
KW - male
KW - moisture
KW - MTT assay
KW - nanofabrication
KW - nonhuman
KW - pore size
KW - porosity
KW - rat
KW - scanning electron microscopy
KW - shear rate
KW - shear stress
KW - skin injury
KW - skinfold thickness
KW - Staphylococcus aureus
KW - surface area
KW - surface property
KW - temperature
KW - tensile strength
KW - torque
KW - viscosity
KW - water absorption
KW - water transport
KW - wettability
KW - wound closure
KW - wound healing
KW - wound healing rate
KW - zone of inhibition
KW - animal
KW - Calendula
KW - Gram negative bacterium
KW - Gram positive bacterium
KW - infrared spectroscopy
KW - wound healing
PB - Elsevier B.V.
SN - 03785173 (ISSN)
C2 - 34563618
LA - English
J2 - Int. J. Pharm.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 35; Correspondence Address: M. Kabiri;
Department of Biotechnology, College of Science, University of Tehran, Tehran, 14155-6455,
Iran; email: mkabiri@ut.ac.ir; CODEN: IJPHD
ER -

TY - JOUR
AU - Miraj, S.
TI - The role of medicinal plants in the treatment of diseases: A systematic review of Calendula
officinalis
PY - 2016
T2 - Der Pharmacia Lettre
VL - 8
IS - 14
SP - 92
EP - 95
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84987926208&partnerID=40&md5=746b
6af3510bbc50bc965568ab4c7d87
AD - Medicinal Plants Research Center, Shahrekord University of Medical Sciences,
Shahrekord, Iran
AB - Calendula, native to southwestern Asia, western Europe, Macaronesia, and the
Mediterranean, is an annual and perennial herbaceous plants belongs to family Asteraceae.
The aim of this study is to overview its pharmacological effects. This review article was carried
out by searching studies in PubMed, Medline, Web of Science, and IranMedex databases up to
2016.totally, of 119 found articles, 40 articles were included. The search terms were "Calendula
officinalis", "therapeutic", "pharmacological", "herbal therapy". Various studies have shown that
Calendula officinalis possess anti-Pancreatitis properties, anti-Diabetic Properties, Anti-fungal
Properties, Cytotoxic activity, anti-Tendon Properties, anti-Colitis Properties. Calendula is widely
used for therapeutic and purposes that trigger its significant value. Various combinations and
numerous medicinal properties of its extract, essential oils, its stems and leaves demand further
and more studies about the other useful and unknown properties of this multipurpose plant.
KW - Calendula officinalis
KW - Herbal therapy
KW - Pharmacological
KW - Therapeutic
KW - Calendula officinalis extract
KW - flavonol
KW - glycoside
KW - lactate dehydrogenase
KW - plant extract
KW - saponin
KW - sesquiterpene
KW - triterpene
KW - unclassified drug
KW - achilles tendon
KW - adipocyte
KW - anticolitis activity
KW - antidiabetic activity
KW - antifungal activity
KW - antiobesity activity
KW - antioxidant activity
KW - antipancreatitis activity
KW - Article
KW - biosynthesis
KW - Calendula officinalis
KW - cell differentiation
KW - cell viability
KW - concentration response
KW - drug activity
KW - drug cytotoxicity
KW - drug efficacy
KW - drug mechanism
KW - drug response
KW - drug safety
KW - enzyme release
KW - flower
KW - human
KW - medicinal plant
KW - memory disorder
KW - mycorrhiza
KW - nonhuman
KW - pancreatitis
KW - phytochemistry
KW - streptozotocin-induced diabetes mellitus
KW - systematic review (topic)
KW - ulcerative colitis
PB - Scholars Research Library
SN - 09755071 (ISSN)
LA - English
J2 - Der Pharm. Lett.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1
ER -

TY - JOUR
AU - Pazhohideh, Z.
AU - Mohammadi, S.
AU - Bahrami, N.
AU - Mojab, F.
AU - Abedi, P.
AU - Maraghi, E.
TI - The effect of Calendula officinalis versus metronidazole on bacterial vaginosis in women: A
double-blind randomized controlled trial
PY - 2018
T2 - Journal of Advanced Pharmaceutical Technology and Research
VL - 9
IS - 1
SP - 15
EP - 19
DO - 10.4103/japtr.JAPTR_305_17
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041114640&doi=10.4103%2fjaptr.JAPT
R_305_17&partnerID=40&md5=5e18bebfeeeb29e39f66fac48991072b
AD - Department of Midwifery, Faculty of Medical Sciences, Shushtar, Iran
AD - Department of Midwifery, Ahvaz Jundishapur University of Medical Sciences, Iran
AD - Faculty of Nursing and Midwifery, Dezful University of Medical Sciences, Dezful, Iran
AD - Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
AD - Department of Midwifery, Menopause Andropause Research Center, Ahvaz Jundishapur
University of Medical Sciences, 13th East Kianpars Ave, 1st Maroon St., No: 46, Ahvaz, Iran
AD - Department of Biostatistics and Epidemiology, School of Public Health, Ahvaz Jundishapur
University of Medical Sciences, Ahvaz, Iran
AB - Bacterial vaginosis (BV) is a common disorder among women of reproductive age. This
study aimed to compare the effect of a Calendula officinalis extract-based cream and
metronidazole on BV among women of reproductive age. In this study, 80 women of
reproductive age with BV were randomly assigned to the C. officinalis (n = 40) or metronidazole
(n = 40). Diagnosis of BV was confirmed when at least 3 of the 4 Amsel criteria were met (pH
>4.5, whitish grey or thin homogeneous discharge, release of a fishy odor on adding 10% KOH,
and detection of clue cells on microscopic examination). For each group, either a methanol
extract of C. officinalis or metronidazole vaginal cream (5 g) was used for 1 week intravaginally,
and all signs and symptoms were assessed 1 week after treatment completion. Before the
intervention, the two groups did not differ significantly with regard to vaginal burning, odor,
dysuria, and dyspareunia, but itching was significantly more common in the C. officinalis group
than in the metronidazole group (22.5% vs. 2.5%, P = 0.01). One week after the intervention, all
women in both groups were free of symptoms, including vaginal itching and burning sensation,
odor, dysuria, and dyspareunia. None of the women in either group suffered any side effects
from C. officinalis or metronidazole. C. officinalis was effective for the treatment of BV in women
of reproductive age, without any side effects. This herb could be recommended for women of
reproductive age who uncomfortable with the potential side effects of synthetic drugs. © 2018
Journal of Advanced Pharmaceutical Technology & Research.
KW - Bacterial
KW - Calendula officinalis
KW - metronidazole
KW - vaginitis
KW - antiinfective agent
KW - Calendula officinalis extract
KW - metronidazole
KW - plant extract
KW - unclassified drug
KW - adult
KW - Article
KW - Calendula officinalis
KW - controlled study
KW - cream
KW - double blind procedure
KW - drug efficacy
KW - dyspareunia
KW - dysuria
KW - female
KW - human
KW - major clinical study
KW - odor
KW - priority journal
KW - randomized controlled trial
KW - vagina discharge
KW - vaginal burning sensation
KW - vaginal pruritus
KW - vaginitis
PB - Wolters Kluwer Medknow Publications
SN - 22314040 (ISSN)
LA - English
J2 - J. Adv. Pharm. Technol. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 11; Correspondence Address: P. Abedi;
Department of Midwifery, Menopause Andropause Research Center, Ahvaz Jundishapur
University of Medical Sciences, Ahvaz, 13th East Kianpars Ave, 1st Maroon St., No: 46, Iran;
email: parvinabedi@ymail.com
ER -

TY - JOUR
AU - Caamal-Herrera, I.O.
AU - Muñoz-Rodríguez, D.
AU - Madera-Santana, T.
AU - Azamar-Barrios, J.A.
TI - Identification of volatile compounds in hydro-alcoholic extracts of calendula officinalis l.
flowers and mimosae tenuiflorae bark using GC/MS
PY - 2016
T2 - International Journal of Applied Research in Natural Products
VL - 9
IS - 1
SP - 20
EP - 30
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958617357&partnerID=40&md5=d725
b906682cceb3c3b2441774a19520
AD - Departamento de Física Aplicada, Centro de investigación y de Estudios Avanzados del
IPN, Unidad Mérida, Km. 6 antigua carretera a Progreso, Cordemex, C.P, Mérida, Yucatán,
97310, Mexico
AD - Cuerpo académico de Química Fundamental y Aplicada, Facultad de Ingeniería Química,
Universidad Autónoma de Yucatán, Tablaje Catastral 13615, Chuburna de Hidalgo Inn, C.P,
Mérida, Yucatán, 97203, Mexico
AD - Laboratorio de Envases, CTAOV, Centro de Investigación en Alimentación y Desarrollo,
Carretera a La Victoria km 0.6 C.P, Hermosillo, Sonora, 83304, Mexico
AB - Calendula officinalis L. and Mimosae tenuiflorae are important plants in the traditional
Mexican ethno-medicine due to that possess biological activities, including anti-inflammatory,
antimicrobial, antioxidant and healing activities. In this study, the volatile compounds present in
the hydro-alcoholic extract of flowers of Calendula officinalis, and from the bark of Mimosae
tenuiflorae were determined using gas chromatography coupled to a mass spectrometer
(GC/MS). The extracts were submitted to solid-phase extraction (SPE) with C18 cartridges in
order to obtain the compounds in a suitable solvent for analysis by gas chromatography and to,
simultaneously, pre-concentrate them. In both extracts, the acetonitrile allowed us to elute a
greater amount (20 to 40) of the volatile compounds. In the hydro-alcoholic extract of Calendula
officinalis flowers, the α-thujone, β-thujone, 4-terpineol, (-)-bornylacetate, eucalyptol, α-cadinol
and α-epi-muurolol compounds were eluted from the SPE cartridge using acetonitrile and
isooctane; α-thujone was the compound with the highest percentage area, but in the
hydro-alcoholic extract of Mimosae tenuiflorae bark, only eucalyptol was recovered in this way.
In this same extract, only resorcinol and N, N-dimethyltryptamine were recovered by elution with
acetonitrile, and these were major compounds together with the (+)-2-bornanone. Industrial
relevance. The anti-tumour and anti-metastatic effects of α-thujone, the antimicrobial and
anti-parasitic properties of 4-terpineol as well as the antifungal activity of α-cadinol, in addition to
the majority presence of these compounds in the hydro-alcoholic extract of Calendula officinalis
flowers, support the prolonged employment of this plant in traditional medicine and potentiates
its application in the pharmaceutical and biomedical areas. This may be through the use of the
hydro-alcoholic extract or using it as a source to obtain volatile compounds. Moreover, the
identification of resorcinol and (+)-2-bornanone in the hydro-alcoholic extract of Mimosae
tenuiflorae bark explains some of the properties attributed to the plant, including the antiseptic
and analgesic effects. © 2008-2016. IJARNP-HS Publication.
KW - Calendula officinalis
KW - GC/MS
KW - Hydro-alcoholic extracts
KW - Mimosae tenuiflorae
KW - SPE
KW - absinthol
KW - alpha cadinol
KW - alpha epi muurolol
KW - beta thuyone
KW - bornyl acetate
KW - Calendula officinalis extract
KW - camphor
KW - cineole
KW - Mimosa tenuiflora extract
KW - n,n dimethyltryptamine
KW - plant extract
KW - resorcinol
KW - terpinen 4 ol
KW - unclassified drug
KW - volatile agent
KW - Article
KW - bark
KW - Calendula officinalis
KW - chemical composition
KW - controlled study
KW - flower
KW - mass fragmentography
KW - Mimosa
KW - Mimosa tenuiflora
KW - solid phase extraction
PB - Healthy Synergies Publications
SN - 19406223 (ISSN)
LA - English
J2 - Int. J. Appl. Res. Nat. Prod.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 3; Correspondence Address: J.A.
Azamar-Barrios; Departamento de Física Aplicada, Centro de investigación y de Estudios
Avanzados del IPN, Unidad Mérida, Mérida, Yucatán, Km. 6 antigua carretera a Progreso,
Cordemex, C.P., 97310, Mexico; email: azamar@mda.cinvestav.mx
ER -

TY - JOUR
AU - Mishra, A.K.
AU - Mishra, A.
AU - Chattopadhyay, P.
TI - Screening of acute and sub-chronic dermal toxicity of Calendula officinalis L essential oil
PY - 2018
T2 - Regulatory Toxicology and Pharmacology
VL - 98
SP - 184
EP - 189
DO - 10.1016/j.yrtph.2018.07.027
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050879711&doi=10.1016%2fj.yrtph.20
18.07.027&partnerID=40&md5=9dd916cf1d89b41ecaadc0e095cadf84
AD - Central Facility of Instrumentation, Faculty of Pharmacy, IFTM University, Lodipur-Rajput,
Moradabad, 244001, India
AD - Pharmaceutical Technology Division, Defense Research Laboratory, DRDO, Tezpur,
784001, India
AB - The objective of the study is to access the safety of Calendula essential oil by studying
acute and sub-chronic dermal toxicity. The dermal toxicities of Calendula essential oil were
evaluated in accordance with OECD guidelines number 402 and 411 respectively. The animals
were exposed to Calendula officinalis (CO) essential oil dose of 20 mL/kg body weight for acute
dermal toxicity, whereas for dermal sub-chronic toxicity study, rats were exposed to CO oil 2.5, 5
and 10 mL/kg body weight, respectively, for 7 times in a week for 90 days. The parameters
studies included CNS stimulation, depression, hematological parameters (RBC, WBC, Hb,
Lymphocyte % etc), biochemical parameters (total protein, albumin, total bilirubin, ALP, AST,
etc), relative organ weight, necropsy and histopathology. In toxicity studies, all animals exhibited
normal behavior without any change in hematology, blood biochemistry, necroscopical and
histopathology. The no observed effect level (NOEL) and no observed adverse effect level
(NOAEL) of CO oil were 2.5 and 10 mg/kg/day, respectively. CO oil is under the herbal
medicinal product according to the European Medicines Agency with the claim of an LD50 value
of 20 mL/kg body weight. The result indicates that CO essential oil did not produce any
significant toxic effects. © 2018 Elsevier Inc.
KW - Acute toxicity
KW - Calendula officinalis essential oil
KW - Dermal toxicity
KW - OECD
KW - Animals
KW - Calendula
KW - Female
KW - Male
KW - No-Observed-Adverse-Effect Level
KW - Oils, Volatile
KW - Rats, Wistar
KW - Skin
KW - Toxicity Tests, Acute
KW - Toxicity Tests, Subchronic
KW - albumin
KW - alkaline phosphatase
KW - aspartate aminotransferase
KW - bilirubin
KW - Calendula officinalis essential oil
KW - essential oil
KW - hemoglobin
KW - protein
KW - unclassified drug
KW - essential oil
KW - acute toxicity
KW - adult
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - Calendula officinalis
KW - chronic toxicity
KW - controlled study
KW - depression
KW - drug safety
KW - erythrocyte count
KW - female
KW - histopathology
KW - LD50
KW - leukocyte count
KW - lymphocyte count
KW - male
KW - nonhuman
KW - organ weight
KW - priority journal
KW - rat
KW - skin toxicity
KW - animal
KW - Calendula
KW - drug effect
KW - no-observed-adverse-effect level
KW - skin
KW - toxicity testing
KW - Wistar rat
PB - Academic Press Inc.
SN - 02732300 (ISSN)
C2 - 30075180
LA - English
J2 - Regul. Toxicol. Pharmacol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 22; Correspondence Address: A.K. Mishra;
Central Facility of Instrumentation, Faculty of Pharmacy, IFTM University, Moradabad,
Lodipur-Rajput, 244001, India; email: arun_azam@rediffmail.com; CODEN: RTOPD
ER -

TY - JOUR
AU - Olennikov, D.N.
AU - Kashchenko, N.I.
AU - Vennos, C.
TI - A new esculetin glycoside from Calendula officinalis (Asteraceae) and its bioactivity
PY - 2017
T2 - Farmacia
VL - 65
IS - 5
SP - 698
EP - 702
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032361444&partnerID=40&md5=517d
48c7d8247f43f6b11df877e48894
AD - Institute of General and Experimental Biology, Siberian Division, Russian Academy of
Science, Sakh’yanovoy Str., 6, Ulan-Ude, Russian Federation
AD - Padma AG, Underfeldstrasse 1, CH-8340, Hinwil, 670047, Switzerland
AB - A new coumarin glycoside, neoisobaisseoside, was isolated from the flowers of Calendula
officinalis L. (Asteraceae), together with three known compounds identified as isobaisseoside,
haploperoside A and haploperoside D. The structure of neoisobaisseoside was characterized as
6,7-dihydroxycoumarin-7-О-(2′-О-α-l-rhamnopyranosyl)-β-d-glycopyranoside or
esculetin-7-O-neohesperiodoside, based on UV-, NMR-spectroscopy and mass-spectrometric
data. Investigation of the biological properties of neoisobaisseoside demonstrated its amylase
and α-glucosidase inhibiting activity as well as its ability to reduce the production of advanced
glycation end-products formed in the Maillard reaction. These facts indicate that
neoisobaisseoside could be a potential anti-diabetic agent. © 2017, Romanian Society for
Pharmaceutical Sciences. All rights reserved.
KW - Antidiabetic activity
KW - Calendula officinalis
KW - Coumarins
KW - Neoisobaisseoside
KW - acarbose
KW - advanced glycation end product
KW - alpha glucosidase
KW - amylase
KW - esculetin
KW - glucose
KW - haploperoside A
KW - haploperoside D
KW - isobaisseoside
KW - neoisobaisseoside
KW - plant glycoside
KW - rhamnose
KW - scopoletin
KW - unclassified drug
KW - antidiabetic activity
KW - Article
KW - Calendula officinalis
KW - carbon nuclear magnetic resonance
KW - controlled study
KW - enzyme inhibition
KW - extraction
KW - flower
KW - high performance liquid chromatography
KW - hydrolysis
KW - IC50
KW - mass fragmentography
KW - nonhuman
KW - proton nuclear magnetic resonance
KW - ultraviolet spectrophotometry
PB - Romanian Society for Pharmaceutical Sciences
SN - 00148237 (ISSN)
LA - English
J2 - Farmacia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 14; Correspondence Address: D.N. Olennikov;
Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science,
Ulan-Ude, Sakh’yanovoy Str., 6, Russian Federation; email: olennikovdn@mail.ru; CODEN:
FRMBA
ER -

TY - JOUR
AU - Verma, P.K.
AU - Raina, R.
AU - Singh, M.
AU - Wazir, V.S.
AU - Kumar, P.
TI - Attenuating potential of calendula officinalis on biochemical and antioxidant parameters in
hepatotoxic rats
PY - 2017
T2 - Indian Journal of Physiology and Pharmacology
VL - 61
IS - 4
SP - 398
EP - 410
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030786366&partnerID=40&md5=14f9e
66ffb1962df335f72c11d574cd5
AD - Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and
Animal Husbandry, R.S. Pura, 181 102, Jammu & Kashmir, India
AD - Division of Veterinary Public Health and Epidemiology, Faculty of Veterinary Science and
Animal Husbandry, R.S. Pura, 181 102, Jammu & Kashmir, India
AD - Division of Veterinary Medicine, Faculty of Veterinary Science and Animal Husbandry, R.S.
Pura, 181 102, Jammu & Kashmir, India
AD - Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122,
UP, India
AB - Attenuating potential of floral extracts of Calendula officinalis on biochemical and
antioxidant parameters in blood and hepatic tissue of acetaminophen (APAP) induced
hepatotoxicity in wistar rats was determined. Increased (P<0.05) plasma activities of
phosphatases, transferases, reduced levels of total proteins and conjugated bilirubin following
administration of APAP indicated acute hepatotoxicity. Hepatotoxic rats also exhibited significant
reduced levels of total thiols (TTH), reduced glutathione (GSH), total antioxidant status (TAS)
and antioxidant enzymes, and increased oxidative stress index (OSI), total oxidant status (TOS)
and malondialdehyde (MDA) levels in blood and hepatic tissue. Treatment with ethanolic floral
extract restored hepatic blood biomarkers, increased (P<0.05) the levels of TTH, GSH, TAS and
antioxidant enzymes, and reduced the levels of MDA, TOS and OSI in blood and hepatic tissue
and histopathological alterations in hepatotoxic rats. Study suggested that ethanolic floral
extract of C. officinalis administrations attenuating the APAP induced oxidative damage in
hepatic tissue. © 2017, Association of Physiologists and Pharmacologists of India. All rights
reserved.
KW - alcohol
KW - antioxidant
KW - bilirubin glucuronide
KW - biological marker
KW - Calendula officinalis extract
KW - glutathione
KW - malonaldehyde
KW - paracetamol
KW - phosphatase
KW - plant extract
KW - protein
KW - silymarin
KW - thiol
KW - transferase
KW - unclassified drug
KW - water
KW - adult
KW - animal experiment
KW - animal model
KW - animal tissue
KW - antioxidant activity
KW - Article
KW - bilirubin blood level
KW - biochemical analysis
KW - blood level
KW - Calendula officinalis
KW - controlled study
KW - disease marker
KW - enzyme blood level
KW - female
KW - flower
KW - histopathology
KW - liver level
KW - male
KW - nonhuman
KW - oxidative stress
KW - protein blood level
KW - rat
KW - toxic hepatitis
KW - Wistar rat
PB - Association of Physiologists and Pharmacologists of India
SN - 00195499 (ISSN)
LA - English
J2 - Indian J. Physiol. Pharmacol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 3; CODEN: IJPPA
ER -

TY - JOUR
AU - Aro, A.A.
AU - Perez, M.O.
AU - Vieira, C.P.
AU - Esquisatto, M.A.M.
AU - Rodrigues, R.A.F.
AU - Gomes, L.
AU - Pimentel, E.R.
TI - Effect of calendula officinalis cream on achilles tendon healing
PY - 2015
T2 - Anatomical Record
VL - 298
IS - 2
SP - 428
EP - 435
DO - 10.1002/ar.23057
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920973464&doi=10.1002%2far.23057
&partnerID=40&md5=a763fda42d7e2a184954a529e96699b4
AD - Department of Structural and Functional Biology, Institute of Biology, State University of
Campinas-UNICAMP, Campinas, São Paulo, Brazil
AD - Biomedical Sciences Graduate Program, Herminio Ometto University Center
(FHO/UNIARARAS), Araras, São Paulo, Brazil
AD - Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), State
University of Campinas-UNICAMP, Campinas, São Paulo, Brazil
AB - In recent years, the scientific community has undertaken research on plant extracts,
searching for compounds with pharmacological activities that can be used in diverse fields of
medicine. Calendula officinalis L. is known to have antioxidant, anti-inflammatory, antibacterial,
and wound healing properties when used to treat skin burns. Therefore, the purpose of this
study was to analyze the effects of C. officinalis on the initial phase of Achilles tendon healing.
Wistar rats were separated in three groups: Calendula (Cal)-rats with a transected tendon were
treated with topical applications of C. officinalis cream and then euthanized 7 days after injury;
Control (C)-rats were treated with only vehicle after transection; and Normal (N)-rats without
tenotomy. Higher concentrations of hydroxyproline (an indicator of total collagen) and
non-collagenous proteins were observed in the Cal group in relation to the C group.
Zymography showed no difference in the amount of the isoforms of metalloproteinase-2 and of
metalloproteinase-9, between C and Cal groups. Polarization microscopy images analysis
showed that the Cal group presented a slightly higher birefringence compared with the C group.
In sections of tendons stained with toluidine blue, the transected groups presented higher
metachromasy as compared with the N group. Immunocytochemistry analysis for
chondroitin-6-sulfate showed no difference between the C and Cal groups. In conclusion, the
topical application of C. officinalis after tendon transection increases the concentrations of
collagen and non-collagenous proteins, as well as the collagen organization in the initial phase
of healing. Anat Rec, 298:428-435, 2015. © 2014 Wiley Periodicals, Inc.
KW - Calendula officinalis
KW - Hydroxyproline
KW - Metalloproteinase
KW - Non-collagenous proteins
KW - Tendon repair
KW - Achilles Tendon
KW - Administration, Topical
KW - Animals
KW - Calendula
KW - Male
KW - Plant Extracts
KW - Rats
KW - Rats, Wistar
KW - Skin Cream
KW - Treatment Outcome
KW - Wound Healing
KW - Calendula
KW - Calendula officinalis
KW - Rattus
KW - Rattus norvegicus
KW - Calendula officinalis extract
KW - chondroitin 6 sulfate
KW - collagen type 1
KW - gelatinase A
KW - gelatinase B
KW - hydroxyproline
KW - isoenzyme
KW - plant extract
KW - tolonium chloride
KW - unclassified drug
KW - plant extract
KW - skin cream
KW - achilles tendon rupture
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - birefringence
KW - Calendula officinalis
KW - controlled study
KW - cream
KW - drug effect
KW - healing
KW - image analysis
KW - immunocytochemistry
KW - male
KW - nonhuman
KW - polarization microscopy
KW - priority journal
KW - rat
KW - zymography
KW - achilles tendon
KW - animal
KW - Calendula
KW - drug effects
KW - isolation and purification
KW - metabolism
KW - pathology
KW - physiology
KW - topical drug administration
KW - treatment outcome
KW - Wistar rat
KW - wound healing
SN - 19328486 (ISSN)
C2 - 25266273
LA - English
J2 - Anat. Rec.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 20; Correspondence Address: A.A. Aro;
Department of Structural and Functional Biology, Institute of Biology, State University of
Campinas-UNICAMP, Campinas, São Paulo, Brazil; email: andreaaro@ig.com.br
ER -

TY - JOUR
AU - Lovecka, P.
AU - Lipov, J.
AU - Thumova, K.
AU - Macurkova, A.
TI - Characterization of biologically active substances from Calendula officinalis
PY - 2017
T2 - Current Pharmaceutical Biotechnology
VL - 18
IS - 14
SP - 1167
EP - 1174
DO - 10.2174/1389201019666180226151910
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047837444&doi=10.2174%2f13892010
19666180226151910&partnerID=40&md5=499df20ebcadfecfc8e521c8a8f7065a
AD - Department of Biochemistry and Microbiology, Faculty of Food and Biochemical
Technology, University of Chemistry and Technology Prague, Prague, 166 28, Czech Republic
AB - Background: The aim of this work was to compare water and organic extracts, infusions
and tinctures from flowers and leaves of Calendula officinalis in terms of their biological activity
and composition. The purpose of work was investigation whether the leaves and stems are
really the waste or they contain interesting substances which could be utilized. Antimicrobial,
antifungal, antioxidant and anti-inflammatory activities were studied. Then, the ability to inhibit
collagenase was studied as well. Cytotoxicity was tested for all the samples on mammalian cell
lines. Methods: To determine the composition of extracts, infusions and tinctures phytochemical
analysis (the set of colour reactions for the detection of groups of biologically active compounds)
was carried out and showed that samples from flowers and leaves contain the same groups of
biologically active substances (proteins and amino acids, reducing sugars, flavonoids, saponins,
phenolics, terpenoids, steroids, glycosides). The antimicrobial activity of tested samples was
proved, where the most sensitive bacterium was Micrococcus luteus and the most sensitive
yeast was Geotrichum candidum. Results: The study of anti-collagenase activity has shown that
the enzymatic reaction of collagenase was affected by all tested samples and their effect was
concentration dependent. Cytotoxicity of water and methanol extracts at cell lines HEK 293T
and HepG2 was observed. Conclusion: Cells HepG2 were more sensitive than cells HEK 293T.
Using cell line RAW 264.7, antiinflammatory activity of all samples was observed. Tincture of
leaves was the most effective. © 2017 Bentham Science Publishers.
KW - Anti-colagenase activity
KW - Anti-inflammatory activity
KW - Antimicrobial activity
KW - Calendula officinalis
KW - Cytotoxicity
KW - Mammalian cells
KW - Phytochemical analysis
KW - Animals
KW - Anti-Infective Agents
KW - Anti-Inflammatory Agents
KW - Antioxidants
KW - Calendula
KW - Cell Line
KW - Cell Survival
KW - Flowers
KW - HEK293 Cells
KW - Hep G2 Cells
KW - Humans
KW - Plant Extracts
KW - Plant Leaves
KW - Calendula officinalis extract
KW - clostridiopeptidase A
KW - collagenase
KW - nitric oxide
KW - plant extract
KW - unclassified drug
KW - antiinfective agent
KW - antiinflammatory agent
KW - antioxidant
KW - plant extract
KW - antifungal activity
KW - antiinflammatory activity
KW - antimicrobial activity
KW - antioxidant activity
KW - Article
KW - biological activity
KW - Calendula officinalis
KW - Clostridium histolyticum
KW - controlled study
KW - cytotoxicity
KW - DPPH radical scavenging assay
KW - enzyme assay
KW - flower
KW - Geotrichum candidum
KW - human
KW - human cell
KW - Micrococcus luteus
KW - nonhuman
KW - phytochemistry
KW - plant leaf
KW - plant stem
KW - ultraviolet spectrophotometry
KW - animal
KW - Calendula
KW - cell line
KW - cell survival
KW - chemistry
KW - drug effect
KW - HEK293 cell line
KW - Hep-G2 cell line
KW - isolation and purification
PB - Bentham Science Publishers B.V.
SN - 13892010 (ISSN)
C2 - 29484986
LA - English
J2 - Curr. Pharm. Biotechnol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 9; Correspondence Address: P. Lovecka;
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology,
University of Chemistry and Technology Prague, Prague, Technicka 3, 166 28, Czech Republic;
email: loveckap@vscht.cz; CODEN: CPBUB
ER -

TY - JOUR
AU - Doligalska, M.
AU - Jóźwicka, K.
AU - Szewczak, L.
AU - Nowakowska, J.
AU - Brodaczewska, K.
AU - Goździk, K.
AU - Pączkowski, C.
AU - Szakiel, A.
TI - Calendula officinalis triterpenoid saponins impact the immune recognition of proteins in
parasitic nematodes
PY - 2021
T2 - Pathogens
VL - 10
IS - 3
C7 - 296
SP - 1
EP - 21
DO - 10.3390/pathogens10030296
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102735517&doi=10.3390%2fpathogen
s10030296&partnerID=40&md5=6c941b48c208a0837aba0b373f3ed900
AD - Department of Parasitology, Faculty of Biology, University of Warsaw, Warsaw, 02‐096,
Poland
AD - Laboratory of Electron and Confocal Microscopy, Faculty of Biology, University of Warsaw,
Warsaw, 02‐096, Poland
AD - Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Warsaw,
02‐096, Poland
AB - The influence of triterpenoid saponins on subcellular morphological changes in the cells of
parasitic nematodes remains poorly understood. Our study examines the effect of oleanolic acid
glucuronides from marigold (Calendula officinalis) on the possible modification of immunogenic
proteins from infective Heligmosomoides polygyrus bakeri larvae (L3). Our findings indicate that
the triterpenoid saponins alter the subcellular morphology of the larvae and prevent recognition
of nematode‐specific proteins by rabbit immune‐IgG. TEM ultrastructure and HPLC analysis
showed that microtubule and cytoskeleton fibres were fragmented by saponin treatment.
MASCOT bioinformatic analysis revealed that in larvae exposed to saponins, the immune
epitopes of their proteins altered. Several mitochondrial and cytoskeleton proteins involved in
signalling and cellular processes were downregulated or degraded. As possible candidates, the
following set of recognised proteins may play a key role in the immunogenicity of larvae:
beta‐tubulin isotype, alpha‐tubulin, myosin, paramyosin isoform‐1, actin, disorganized muscle
protein‐1, ATP‐synthase, beta subunit, carboxyl transferase domain protein, glutamate
dehydrogenase, enolase (phosphopyruvate hydratase), fructose‐bisphosphate aldolase 2,
tropomyosin, arginine kinase or putative chaperone protein DnaK, and galactoside‐binding
lectin. Data are available via ProteomeXchange with identifier PXD024205. © 2021 by the
authors. Licensee MDPI, Basel, Switzerland.
KW - Protein patterns
KW - TEM nematode ultrastructure
KW - Triterpenoid saponins
KW - actin
KW - alpha tubulin
KW - arginine kinase
KW - beta tubulin
KW - carboxyltransferase
KW - chaperone
KW - disorganized muscle protein 1
KW - enolase
KW - epitope
KW - fructose bisphosphate aldolase
KW - galaptin
KW - glutamate dehydrogenase
KW - immunoglobulin G
KW - muscle protein
KW - myosin
KW - oleanolic acid derivative
KW - oleanolic acid glucuronide
KW - paramyosin
KW - proton transporting adenosine triphosphate synthase
KW - putative chaperone protein DnaK
KW - triterpenoid
KW - tropomyosin
KW - unclassified drug
KW - affinity chromatography
KW - antigen recognition
KW - Article
KW - beta chain
KW - binding affinity
KW - blood sampling
KW - Calendula officinalis
KW - cell function
KW - cell viability
KW - cytoskeleton
KW - data analysis
KW - down regulation
KW - egg
KW - gene ontology
KW - glycolysis
KW - Haemonchus contortus
KW - Heligmosomoides bakeri
KW - high performance liquid chromatography
KW - immunization
KW - immunogenicity
KW - larva
KW - liquid chromatography-mass spectrometry
KW - microtubule
KW - mouse
KW - nonhuman
KW - oocyte maturation
KW - polyacrylamide gel electrophoresis
KW - protein analysis
KW - protein domain
KW - protein expression
KW - protein isolation
KW - proteomics
KW - rabbit breed
KW - signal transduction
KW - staining
KW - thin layer chromatography
KW - transmission electron microscopy
KW - ultrastructure
KW - unfolded protein response
PB - MDPI AG
SN - 20760817 (ISSN)
LA - English
J2 - Pathogens
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: M. Doligalska;
Department of Parasitology, Faculty of Biology, University of Warsaw, Warsaw, 02‐096, Poland;
email: m.doligalska@biol.uw.edu.pl
ER -

TY - JOUR
AU - Das, K.
AU - Deb, S.
AU - Karanth, T.
TI - Phytochemical screening and metallic ion content and its impact on the antipsoriasis
activity of aqueous leaf extracts of Calendula officinalis and Phlebodium decumanum in an
animal experiment model
ST - Calendula officinalis ve Phlebodium decumanum sulu yaprak ekstreleri üzerinde
fitokimyasal tarama, metalik iyon içeriği ve hayvan deneyi modelinde antipsoriasis etkisi
PY - 2019
T2 - Turkish Journal of Pharmaceutical Sciences
VL - 16
IS - 3
SP - 292
EP - 302
DO - 10.4274/tjps.galenos.2018.44265
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071858654&doi=10.4274%2ftjps.galen
os.2018.44265&partnerID=40&md5=970a1ff1eb285e0dd3c3adf685206b90
AD - Krupanidhi College of Pharmacy, Bangalore, India
AB - Objectives: The aim of this study was to evaluate the influence of metal ions present in soil
as well as in leaf samples of Calendula officinalis and Phlebodium decumanum for the treatment
of psoriasis. Materials and Methods: To meet the objective, soil and leaf samples were
estimated for metal ions by atomic absorption spectrophotometer to determine the influence in
antipsoriatic activity. Thereafter imiquimod-induced dermatitis lesions were created in grouped
mice. Two plant extracts (aqueous) separately as well as in combinations and standard
Retino-A (0.05%) were used. Psoriasis severity index (PSI) was evaluated according to the
phenotypic (redness, erythema, and scales) and histological features (epidermal thickness).
Further content of phytochemicals in terms of extract was correlated with the effect of psoriasis
activity. Results: We observed redness, erythema, and scales and the histological features and
found a progressive reduction (P<0.05) in the severity of psoriatic lesions (redness, erythema,
and scales) from days 7 to 21 and decreased epidermal thickness in animals treated with
combined extracts at a dose of 200 mg/kg b.w. Furthermore, plant samples procured from the
Nandi Hills, Bangalore, showed better uptake of metals with respect to Fe (2.05 mg/kg), Cu
(0.78 mg/kg), and Zn (1.12 mg/kg), which showed a positive impact on procurement of
maximum amount of extracts that further correlated with the activity, indicating a significant
reduction in psoriatic lesions. Conclusion: The results revealed that the significant
dose-dependent antipsoriasis activity of combined aqueous extracts of C. officinalis and P.
decumanum as well as metal ions had an impact on the procurement of extracts and said
activity. © Turk J Pharm Sci, Published by Galenos Publishing House.
KW - Calendula officinalis
KW - Correlation
KW - Epidermal thickness
KW - Phlebodium decumanum
KW - PSI
KW - Psoriasis
KW - alkaloid
KW - antipsoriasis agent
KW - arsenic
KW - cadmium
KW - Calendula extract
KW - copper
KW - flavonoid
KW - imiquimod
KW - iron
KW - metal ion
KW - Phlebodium decumanum extract
KW - phytochemical
KW - plant extract
KW - retino a
KW - retinoic acid
KW - saponin
KW - unclassified drug
KW - zinc
KW - acute toxicity
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - atomic absorption spectrometry
KW - Calendula officinalis
KW - controlled study
KW - dermatitis
KW - drug uptake
KW - erythema
KW - histology
KW - histopathology
KW - mouse
KW - nonhuman
KW - Phlebodium decumanum
KW - phytochemistry
KW - plant leaf
KW - Polypodiaceae
KW - Psoriasis Severity Index
KW - scoring system
KW - skin irritation
KW - skin redness
KW - skinfold thickness
KW - soil acidity
KW - soil analysis
KW - toxicity testing
PB - Turkish Pharmacists Association
SN - 1304530X (ISSN)
LA - English
J2 - Turk. J. Pharm. Sci.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 3; Correspondence Address: K. Das; Krupanidhi
College of Pharmacy, Bangalore, India; email: drkkdsd@gmail.com
ER -

TY - JOUR
AU - Okuma, C.H.
AU - Andrade, T.A.M.
AU - Caetano, G.F.
AU - Finci, L.I.
AU - Maciel, N.R.
AU - Topan, J.F.
AU - Cefali, L.C.
AU - Polizello, A.C.M.
AU - Carlo, T.
AU - Rogerio, A.P.
AU - Spadaro, A.C.C.
AU - Isaac, V.L.B.
AU - Frade, M.A.C.
AU - Rocha-Filho, P.A.
TI - Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis)
as a potential modern wound dressing
PY - 2015
T2 - European Journal of Pharmaceutical Sciences
VL - 71
SP - 62
EP - 72
DO - 10.1016/j.ejps.2015.01.016
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923869122&doi=10.1016%2fj.ejps.201
5.01.016&partnerID=40&md5=2c884d65bda15e287040d9b3badaa637
AD - Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirao
Preto, University of Sao Paulo, Avenida do Café, s/n, Ribeirão Preto, São Paulo, 14040-903,
Brazil
AD - Division of Dermatology, Department of Internal Medicine, University of Sao Paulo, Brazil
AD - State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life
Sciences, Peking University, Beijing, China
AD - Dana-Farber Cancer Institute, Harvard Medical School, Boston, United States
AD - School of Pharmaceutical Sciences of Araraquara, Sao Paulo State University (UNESP),
Brazil
AD - Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirao
Preto, University of Sao Paulo, Brazil
AD - Pulmonary and Critical Care Medicine Division, Department of Internal Medicine, Brigham
and Women's Hospital, Boston, 02115, MA, United States
AD - Federal University of Triangulo Mineiro, Uberaba, Brazil
AB - Appropriate therapeutics for wound treatments can be achieved by studying the
pathophysiology of tissue repair. Here we develop formulations of lamellar gel phase (LGP)
emulsions containing marigold (Calendula officinalis) oil, evaluating their stability and activity on
experimental wound healing in rats. LGP emulsions were developed and evaluated based on a
phase ternary diagram to select the best LGP emulsion, having a good amount of anisotropic
structure and stability. The selected LGP formulation was analyzed according to the intrinsic and
accelerated physical stability at different temperatures. In addition, in vitro and in vivo studies
were carried out on wound healing rats as a model. The LGP emulsion (15.0% marigold oil;
10.0% of blend surfactants and 75.0% of purified water [w/w/w]) demonstrated good stability
and high viscosity, suggesting longer contact of the formulation with the wound. No cytotoxic
activity (50-1000 μg/mL) was observed in marigold oil. In the wound healing rat model, the LGP
(15 mg/mL) showed an increase in the leukocyte recruitment to the wound at least on days 2
and 7, but reduced leukocyte recruitment after 14 and 21 days, as compared to the control.
Additionally, collagen production was reduced in the LGP emulsion on days 2 and 7 and further
accelerated the process of re-epithelialization of the wound itself. The methodology utilized in
the present study has produced a potentially useful formulation for a stable LGP
emulsion-containing marigold, which was able to improve the wound healing process. © 2015
Elsevier B.V.
KW - Calendula officinalis oil
KW - Lamellar gel phase emulsion
KW - Liquid crystal
KW - Stability tests
KW - Wound healing
KW - Animals
KW - Apoptosis
KW - Bandages
KW - Calendula
KW - Cell Line
KW - Collagen
KW - Emulsions
KW - Gels
KW - Male
KW - Mice
KW - Necrosis
KW - Plant Oils
KW - Rats, Wistar
KW - Skin
KW - Surface-Active Agents
KW - Water
KW - Wound Healing
KW - collagen
KW - marigold oil
KW - nonionic surfactant
KW - plant medicinal product
KW - purified water
KW - unclassified drug
KW - vegetable oil
KW - collagen
KW - emulsion
KW - gel
KW - surfactant
KW - vegetable oil
KW - water
KW - animal cell
KW - animal experiment
KW - animal tissue
KW - anisotropy
KW - apoptosis
KW - Article
KW - Calendula officinalis
KW - cell viability
KW - collagen synthesis
KW - controlled study
KW - cytotoxicity
KW - drug formulation
KW - drug stability
KW - emulsion
KW - epithelization
KW - experimental surgical wound
KW - flow kinetics
KW - in vitro study
KW - in vivo study
KW - lamellar gel phase emulsion
KW - leukocyte
KW - male
KW - marigold
KW - nonhuman
KW - priority journal
KW - rat
KW - room temperature
KW - viscosity
KW - wound dressing
KW - wound healing
KW - animal
KW - bandage
KW - Calendula
KW - cell line
KW - chemically induced
KW - chemistry
KW - drug effects
KW - emulsion
KW - gel
KW - injuries
KW - metabolism
KW - mouse
KW - necrosis
KW - pathology
KW - skin
KW - Wistar rat
PB - Elsevier
SN - 09280987 (ISSN)
C2 - 25684193
LA - English
J2 - Eur. J. Pharm. Sci.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 39; CODEN: EPSCE
ER -

TY - JOUR
AU - Sharifi, P.
AU - Bidabadi, S.S.
AU - Zaid, A.
AU - Abdel Latef, A.A.H.
TI - Efficacy of multi-walled carbon nanotubes in regulating growth performance, total
glutathione and redox state of Calendula officinalis L. cultivated on Pb and Cd polluted soil
PY - 2021
T2 - Ecotoxicology and Environmental Safety
VL - 213
C7 - 112051
DO - 10.1016/j.ecoenv.2021.112051
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101054253&doi=10.1016%2fj.ecoenv.2
021.112051&partnerID=40&md5=5d482f5299739db8f9e3ce1eea1050e1
AD - Department of Agricultural Extension and Education, Higher Education Center Shahid
Bakeri Miyandoab, Urmia University, Urmia, 94171-71946, Iran
AD - Department of Horticulture, College of Agriculture, Isfahan University of Technology,
Isfahan, 84156-83111, Iran
AD - Plant Physiology and Biochemistry Section, Department of Botany, Aligarh Muslim
University, Aligarh, 202002, India
AD - Botany and Microbiology Department, Faculty of Science, South Valley University, Qena,
83523, Egypt
AB - Multi-walled carbon nanotubes (MWCNTs) have recently attracted huge attention to their
impacts on the environment and plants. Therefore, this experiment was conducted to investigate
the responses of lead (Pb) and cadmium (Cd) exposed pot marigold plants to various levels of
MWCNT. Calendula officinalis (L.) seedlings were cultivated in Pb and Cd-polluted soils with
exposure to 0, 50, 100, 250, 500 and 1000 mg L−1 of MWCNT. The results demonstrated that
foliar-applied MWCNT up to 250 mg L−1 not only alleviated Pb and Cd-induced toxicity by
reducing oxidative damage and boosting both enzymatic and non-enzymatic antioxidant
defense system but also promoted the phytoremediation property of pot marigold plants by
enhancing the accumulation of both Pb and Cd from the soil. Interestingly, oxidative damage
exacerbation and both Pb and Cd accumulation reduction were noticed in pot marigold
seedlings exposed to 500 and 1000 mg L−1 MWCNTs. The findings of this study clearly showed
that the use of appropriate concentrations of MWCNTs in increasing the phytoremediation
properties of pot marigold was justified, while the use of high concentrations is toxic to the plant
and intensifies the toxic effects of heavy metals (HMs) on plant physiology. This study provides
a novel method to facilitate the phytoremediation of HMs polluted soils using MWCNT as well as
explores the potential risks of these nanoparticles to the plants. © 2021 The Authors
KW - Antioxidant defense system
KW - Heavy metals
KW - Oxidative stress
KW - Phytoremediation
KW - Antioxidants
KW - Biodegradation, Environmental
KW - Cadmium
KW - Calendula
KW - Glutathione
KW - Lead
KW - Metals, Heavy
KW - Nanotubes, Carbon
KW - Oxidation-Reduction
KW - Oxidative Stress
KW - Seedlings
KW - Soil
KW - Soil Pollutants
KW - Calendula officinalis
KW - ascorbate peroxidase
KW - cadmium
KW - catalase
KW - dehydroascorbic acid reductase
KW - glutathione
KW - glutathione reductase
KW - hydrogen peroxide
KW - lead
KW - monodehydroascorbate reductase
KW - multi walled nanotube
KW - oxidoreductase
KW - superoxide dismutase
KW - unclassified drug
KW - antioxidant
KW - cadmium
KW - carbon nanotube
KW - glutathione
KW - heavy metal
KW - lead
KW - angiosperm
KW - antioxidant
KW - cadmium
KW - carbon nanotube
KW - defense mechanism
KW - growth response
KW - lead
KW - oxidative stress
KW - phytoremediation
KW - redox conditions
KW - soil pollution
KW - Article
KW - bioaccumulation
KW - Calendula officinalis
KW - concentration (parameter)
KW - controlled study
KW - enzyme activity
KW - leaf tissue
KW - nonhuman
KW - oxidative stress
KW - phytoremediation
KW - phytotoxicity
KW - plant growth
KW - plant leaf
KW - plant physiology
KW - risk
KW - seedling
KW - soil pollution
KW - analysis
KW - bioremediation
KW - Calendula
KW - drug effect
KW - metabolism
KW - oxidation reduction reaction
KW - oxidative stress
KW - soil
KW - soil pollutant
KW - toxicity
PB - Academic Press
SN - 01476513 (ISSN)
C2 - 33601169
LA - English
J2 - Ecotoxicol. Environ. Saf.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 12; Correspondence Address: A.A.H. Abdel
Latef; Botany and Microbiology Department, Faculty of Science, South Valley University, Qena,
83523, Egypt; email: moawad76@gmail.com; CODEN: EESAD
ER -

TY - JOUR
AU - Serra, A.P.
AU - Marchetti, M.E.
AU - Vieira, M.C.
AU - Robaina, A.D.
AU - Veronesi, C.O.
AU - Nascimento, J.M.
AU - Matos, F.
AU - Conrad, V.A.
AU - Morais, H.S.
AU - Guimarães, F.C.N.
TI - Nitrogen nutritional efficiency and biomass production in Calendula officinalis L.
(Asteraceae) under greenhouse conditions
ST - Eficiência nutricional do Nitrogênio e produção de biomassa em Calendula officinalis L.
(Asteraceae) em condições de casa de vegetação
PY - 2013
T2 - Revista Brasileira de Plantas Medicinais
VL - 15
IS - 1
SP - 78
EP - 85
DO - 10.1590/S1516-05722013000100011
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875967462&doi=10.1590%2fS1516-05
722013000100011&partnerID=40&md5=a2ed801e53a86a9a3d0df1367162e1ac
AD - Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Gado de
Corte, EMBRAPA-CNPGC, CEP: 79002-970, Campo Grande, Vila Popular, Caixa Postal 154,
Brazil
AD - Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Dourados,
Brazil
AD - Universidade Federal da Grande Dourados, FCA/UFGD, Brazil
AD - Universidade Federal da Grande Dourados, FCA/UFGD, CEP: 79804-970, Dourados,
Caixa Postal 533, Brazil
AB - The aim of this study was to evaluate the effect of both the application of different nitrogen
levels on the soil and the harvest periods on nitrogen nutritional efficiency and biomass
production in Calendula officinalis L. (Asteraceae) under greenhouse conditions. The employed
substrate was 0-20cm layer of Oxisol. Experimental design was completely randomized, with
four replicates. Treatments consisted of four N levels (0, 21, 42 and 84 mg dm-3) and five
harvest periods (15, 30, 45, 60 and 90 days after transplanting). N levels in Calendula officinalis
inflorescences reduced with the harvest periods, and such a reduction was probably due to the
lower efficiency of absorption of this element by the plant and to the lower N translocation to
inflorescences. We can conclude that the evaluation periods influenced the response of plants
to uptake efficiency (UE), and the maximal UE for N was found at 51 days after transplanting of
seedlings. There was an increase in N translocation from the shoot (leaves and stems) to
inflorescences, which has as consequence N reduction in the shoot. Calendula officinalis
biomass production increased with the harvest periods and with the increase in the N levels
applied on the soil. N nutritional efficiency was influenced by both the evaluation periods and the
N levels present on the soil.
KW - Medicinal plants
KW - Mineral nutrition
KW - Nitrogen fertilization
KW - Asteraceae
KW - Calendula officinalis
KW - nitrogen
KW - absorption
KW - article
KW - biomass production
KW - Calendula officinalis
KW - greenhouse effect
KW - harvest period
KW - nonhuman
KW - nutritional assessment
KW - soil
SN - 1983084X (ISSN)
LA - English
J2 - Rev. Bras. Plantas Med.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: A. P. Serra;
Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Gado de Corte,
EMBRAPA-CNPGC, CEP: 79002-970, Campo Grande, Vila Popular, Caixa Postal 154, Brazil;
email: ademar.serra@embrapa.br; CODEN: RBPMF
ER -

TY - JOUR
AU - Mubashar Sabir, S.
AU - Khan, M.F.
AU - Rocha, J.B.T.
AU - Boligon, A.A.
AU - Athayde, M.L.
TI - Phenolic Profile, Antioxidant Activities and Genotoxic Evaluations of Calendula officinalis
PY - 2015
T2 - Journal of Food Biochemistry
VL - 39
IS - 3
SP - 316
EP - 324
DO - 10.1111/jfbc.12132
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984558790&doi=10.1111%2fjfbc.12132
&partnerID=40&md5=a9787a9a942163df25c7f74bbdea5333
AD - Department of Chemistry, University of Poonch, Rawalakot, Azad Kashmir, 92-12350,
Pakistan
AD - Department of Plant Breeding and Molecular Geneticts, University of Poonch, Rawalakot,
Azad Kashmir, 92-12350, Pakistan
AD - Departmento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de
Santa Maria, Santa Maria, Brazil
AD - Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal
University of Santa Maria, Santa Maria, Brazil
AB - Our study aims to evaluate the antioxidant and genotoxic activities obtained from hot
water extracts of flowers and leaves of Calendula officinalisLinn. (Compositae). The extracts
effectively prevented the lipid peroxidation induced by different prooxidants (10μM FeSO4 and
5μM sodium nitroprusside) in rat liver and brain homogenates. Moreover, the free radical
scavenging activities of the extracts were evaluated by the quenching of
2,2-diphenyl-1-picrylhydrazyl (IC50=184.16μg/mL) and deoxyribose (IC50=28.1μg/mL) assays.
The results obtained on alkaline comet assay revealed that exposure of human lymphocytes to
aqueous extract of flower at a relatively high concentration (0.4mg/mL) did not induce genotoxic
effects. The major phenolic acids, some flavonoid aglycone and glycosides, were identified in
flower and leaves by high performance liquid chromatography. These results indicate that
C.officinalis has a significant antioxidant activity and can be effectively utilized against oxidative
stress-related diseases. Practical Applications: C.officinalis is a medicinal plant which has been
used in traditional medicine for skin complaints, wounds and burns, conjunctivitis, poor eyesight,
menstrual irregularities, varicose veins, hemorrhoids and duodenal ulcers. As oxidative stress
has been implicated as one of the inducing factors for the development of various metabolic
disorders, the present study proposes to evaluate the antioxidant potential of flower and leaf
extracts of plant, which may prove to be beneficial against these disorders. © 2015 Wiley
Periodicals, Inc.
KW - Asteraceae
KW - Calendula
KW - Calendula officinalis
KW - Rattus
KW - Assays
KW - Free radicals
KW - High performance liquid chromatography
KW - Liquid chromatography
KW - 2 ,2-diphenyl-1-picrylhydrazyl
KW - Alkaline comet assay
KW - Anti-oxidant activities
KW - Free radical scavenging activity
KW - Genotoxic activity
KW - Genotoxic evaluation
KW - Hot water extracts
KW - Sodium nitroprusside
KW - Antioxidants
PB - Blackwell Publishing Ltd
SN - 01458884 (ISSN)
LA - English
J2 - J. Food Biochem.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 15; Correspondence Address: S. Mubashar
Sabir; Department of Chemistry, University of Poonch, Rawalakot, Azad Kashmir, 92-12350,
Pakistan; email: mubashersabir@yahoo.com
ER -

TY - JOUR
AU - Marian, E.
AU - Vicaș, L.G.
AU - Tunde, J.
AU - Mureșan, M.
AU - Stan, R.L.
AU - Sevastre, B.
AU - Diaconeasa, Z.
AU - Ionescu, C.
AU - Hangan, A.C.
TI - A comparative study on the biologic activity of Centaurea Cyanus Versus Calendula
Officinalis
PY - 2017
T2 - Farmacia
VL - 65
IS - 6
SP - 940
EP - 946
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85039867418&partnerID=40&md5=6077
0b827f22220103164ba8896eea3d
AD - University of Oradea, Medicine and Pharmacy Faculty, Department of Pharmacy, 29
Nicolae Jiga Street, Oradea, 410028, Romania
AD - University of Oradea, Medicine and Pharmacy Faculty, Department of Preclinical
Sciences, 10 1st December Square, Oradea, 410068, Romania
AD - Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department
of Pharmaceutical Biochemistry and Clinical Laboratory, 6 Pasteur Street, Cluj-Napoca, 400012,
Romania
AD - University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine,
Paraclinic/Clinic Department, 3-5 Calea Mănăștur Street, Cluj-Napoca, 400372, Romania
AD - Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department
of Inorganic Chemistry, 12 Ion Creangă Street, Cluj-Napoca, 400010, Romania
AB - Considering that the therapeutic activity of Centaurea cyanus is less known, in the present
study we aimed to evaluate its biological activity compared to Calendula officinalis. The
alcoholic extracts from the two vegetal products were evaluated in terms of chemical
composition, the concentrations being expressed in total polyphenols (gallic acid) and total
flavonoids (quercetin). The studies carried out have shown the protective effect of the plant
extracts against free radicals (superoxide dismutase - SOD-like activity in vivo), their antioxidant
capacity (DPPH, FRAP, CUPRAC methods) and cytotoxicity assays. Centaurea cyanus
possesses superior biological activities versus Calendula officinalis. © 2017, Romanian Society
for Pharmaceutical Sciences. All rights reserved.
KW - Antioxidant
KW - Cytotoxicity
KW - SOD-mimetic activity
KW - alcohol
KW - Calendula officinalis flower extract
KW - Centaurea cyanus flower extract
KW - gallic acid
KW - phenol
KW - plant extract
KW - quercetin
KW - superoxide dismutase
KW - unclassified drug
KW - animal cell
KW - antioxidant assay
KW - Article
KW - cell culture
KW - colorimetry
KW - comparative study
KW - controlled study
KW - cupric reducing antioxidant capacity
KW - DPPH radical scavenging assay
KW - enzyme activity
KW - ferric reducing antioxidant power assay
KW - IC50
KW - MTT assay
KW - nonhuman
KW - Saccharomyces cerevisiae
KW - spectrophotometry
PB - Romanian Society for Pharmaceutical Sciences
SN - 00148237 (ISSN)
LA - English
J2 - Farmacia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 15; Correspondence Address: R.L. Stan; Iuliu
Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of
Pharmaceutical Biochemistry and Clinical Laboratory, Cluj-Napoca, 6 Pasteur Street, 400012,
Romania; email: roxanaluc@yahoo.com; CODEN: FRMBA
ER -

TY - JOUR
AU - Nicolaus, C.
AU - Sievers-Engler, A.
AU - Murillo, R.
AU - D'Ambrosio, M.
AU - Lämmerhofer, M.
AU - Merfort, I.
TI - Mastering analytical challenges for the characterization of pentacyclic triterpene mono- and
diesters of Calendula officinalis flowers by non-aqueous C30 HPLC and hyphenation with
APCI-QTOF-MS
PY - 2016
T2 - Journal of Pharmaceutical and Biomedical Analysis
VL - 118
SP - 195
EP - 205
DO - 10.1016/j.jpba.2015.10.025
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946926572&doi=10.1016%2fj.jpba.201
5.10.025&partnerID=40&md5=62a7e3f09c2c64adf9808496d2f3f973
AD - Institute of Pharmaceutical Sciences, Pharmaceutical Biology and Biotechnology,
University of Freiburg, Stefan-Meier-Strasse 19, Freiburg, D-79104, Germany
AD - Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8,
Tübingen, D-72076, Germany
AD - Escuela de Quimica and CIPRONA, Universidad de Costa Rica, San Jose, 2060, Costa
Rica
AD - Laboratorio di Chimica Bioorganica, Università degli Studi di Trento, Via Sommarive, 14,
Italy
AB - Pentacyclic triterpene mono- and diesters have been isolated from Calendula officinalis
flowers. GC-MS, APCI-Exactive Orbitrap HR-MS and NMR allowed to identify the triterpene
skeleton in various samples (different triterpene mixtures from Calendula n-hexane extract).
NMR provided evidence that triterpene diesters are present in the samples as well. However,
the corresponding quasi-molecular ions could not be detected by APCI-Exactive Orbitrap
HR-MS. Instability of triterpene diesters and loss of a fatty acid residue, respectively, in the
ion-source made their MS detection challenging. Thus, a set of new APCI-QTOF-MS methods
(using the TripleTOF 5600+ mass spectrometer) were developed which made it eventually
possible to solve this problem and confirm the diester structures by MS via quasi-molecular ion
[M+H]+ detection. Direct infusion APCI-QTOF MS experiments in MS/MS high sensitivity scan
mode with low collision energy and multi-channel averaging acquisition (MCA) allowed the
detection of quasi-molecular ions of triterpene diesters for the first time and unequivocally
confirmed the presence of faradiol 3,16-dimyristate and -dipalmitate, as well as the
corresponding mixed diesters faradiol 3-myristate,16-palmitate and faradiol
3-palmitate,16-myristate. Preferential loss of the fatty acid in 16-position made it possible to
distinguish the mixed diesters by MS/MS spectra. Their chromatographic separations turned out
to be challenging due to their bulkiness and extended molecular dimensions. However,
separation could be achieved by an uncommon non-aqueous RPLC mode with an in-house
synthesized C30 phase. Finally, two (U)HPLC-APCI-QTOF-MS methods with C18- and
C30-based non-aqueous RPLC provided suitable, sensitive assays to monitor the presence of
monoesters and diesters of various triterpenes (faradiol, maniladiol, arnidiol, arnitriol A and
lupane-3β,16β,20-triol esters) in the n-hexane extract of C. officinalis with high mass resolution
and good mass accuracy. © 2015 Elsevier B.V.
KW - (U)HPLC-APCI-QTOF-MS
KW - C30 stationary phase
KW - Calendula officinalis
KW - Non-aqueous RPLC
KW - Triterpene mono- and novel diesters
KW - Calendula
KW - Chromatography, High Pressure Liquid
KW - Flowers
KW - Pentacyclic Triterpenes
KW - Plant Extracts
KW - Tandem Mass Spectrometry
KW - aranidiol 3 myristate
KW - arnidiol 3 palmitate
KW - arnitriol a 3 myristate
KW - arnitriol a 3 palmitate
KW - faradiol
KW - faradiol 3 myristate
KW - faradiol 3 palmitate
KW - fatty acid
KW - lupane 3beta,16beta,20 triol 3 myristate
KW - lupane 3beta,16beta,20 triol 3 palmitate
KW - maniladiol 3 myristate
KW - maniladiol 3 palmitate
KW - pentacyclic triterpene
KW - triterpene
KW - unclassified drug
KW - pentacyclic triterpene
KW - plant extract
KW - analytic method
KW - Article
KW - atmospheric pressure chemical ionization
KW - Calendula officinalis
KW - chemical analysis
KW - chemical reaction
KW - drug isolation
KW - drug structure
KW - flower
KW - high performance liquid chromatography
KW - nonhuman
KW - nuclear magnetic resonance spectroscopy
KW - phase separation
KW - priority journal
KW - reversed phase liquid chromatography
KW - sensitivity analysis
KW - separation technique
KW - thin layer chromatography
KW - time of flight mass spectrometry
KW - Calendula
KW - chemistry
KW - high performance liquid chromatography
KW - procedures
KW - tandem mass spectrometry
PB - Elsevier
SN - 07317085 (ISSN)
C2 - 26562182
LA - English
J2 - J. Pharm. Biomed. Anal.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; CODEN: JPBAD
ER -

TY - JOUR
AU - Loescher, C.M.
AU - Morton, D.W.
AU - Razic, S.
AU - Agatonovic-Kustrin, S.
TI - High performance thin layer chromatography (HPTLC) and high performance liquid
chromatography (HPLC) for the qualitative and quantitative analysis of Calendula
officinalis-Advantages and limitations
PY - 2014
T2 - Journal of Pharmaceutical and Biomedical Analysis
VL - 98
SP - 52
EP - 59
DO - 10.1016/j.jpba.2014.04.023
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901606247&doi=10.1016%2fj.jpba.201
4.04.023&partnerID=40&md5=80b470a2436aeb7c08a98856f77c5347
AD - School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La
Trobe University, Bendigo 3550, VIC, Edwards Rd, Australia
AD - Department of Analytical Chemistry, Faculty of Pharmacy, University of Belgrade, 11221
Belgrade, Vojvode Stepe 450, Serbia
AB - Chromatography techniques such as HPTLC and HPLC are commonly used to produce a
chemical fingerprint of a plant to allow identification and quantify the main constituents within the
plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative
analysis of the major constituents of Calendula officinalis and to investigate the effect of different
extraction techniques on the C. officinalis extract composition from different parts of the plant.
The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to
be more accurate for quantitative analysis. A combination of the two methods may be useful in a
quality control setting as it would allow rapid qualitative analysis of herbal material while
maintaining accurate quantification of extract composition. © 2014 Elsevier B.V.
KW - Calendula
KW - Fingerprint profiling
KW - HPLC
KW - HPTLC
KW - Quality control
KW - Calendula
KW - Chromatography, High Pressure Liquid
KW - Chromatography, Thin Layer
KW - Plant Extracts
KW - Quality Control
KW - acetic acid
KW - acetic acid ethyl ester
KW - antioxidant
KW - calendula officinalis extract
KW - hexane
KW - plant extract
KW - tagetes erecta extract
KW - unclassified drug
KW - water
KW - plant extract
KW - antioxidant activity
KW - article
KW - Calendula officinalis
KW - controlled study
KW - densitometry
KW - flower head
KW - high performance liquid chromatography
KW - high performance thin layer chromatography
KW - light scattering
KW - nonhuman
KW - planar chromatography
KW - plant leaf
KW - plant seed
KW - priority journal
KW - qualitative analysis
KW - quantitative analysis
KW - Tagetes erecta
KW - Calendula
KW - chemistry
KW - high performance liquid chromatography
KW - procedures
KW - quality control
KW - thin layer chromatography
PB - Elsevier
SN - 07317085 (ISSN)
C2 - 24880991
LA - English
J2 - J. Pharm. Biomed. Anal.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 46; Correspondence Address: S.
Agatonovic-Kustrin; School of Pharmacy and Applied Science, La Trobe Institute of Molecular
Sciences, La Trobe University, Bendigo 3550, VIC, Edwards Rd, Australia; email:
s.kustrin@latrobe.edu.au; CODEN: JPBAD
ER -

TY - JOUR
AU - Deuschle, V.C.K.N.
AU - Deuschle, R.A.N.
AU - Piana, M.
AU - Boligon, A.A.
AU - Bortoluzzi, M.R.B.
AU - Dal Prá, V.
AU - Dolwisch, C.B.
AU - Lima, F.O.
AU - Carvalho, L.M.
AU - Athayde, M.L.
TI - Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of
Calendula officinalis L. leaves
ST - Avaliação fitoquímica e capacidade antioxidante e fotoprotetora in vitro das folhas de
Calendula officinalis L.
PY - 2015
T2 - Revista Brasileira de Plantas Medicinais
VL - 17
IS - 4
SP - 693
EP - 701
DO - 10.1590/1983-084X/14_055
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955275478&doi=10.1590%2f1983-084
X%2f14_055&partnerID=40&md5=5a2cc7950a17be95015bf0a0dcfc6acc
AD - Department of Industrial Pharmacy (Phytochemical Research Laboratory), Federal
University of Santa Maria, Center for Health Sciences, Build 26, Santa Maria, ZIP 97105-900,
RS, Brazil
AD - Department of Chemistry (Analisys Chemistry Laboratory – Lachem), Federal University of
Santa Maria, Build 17, Santa Maria, ZIP 97105-900, RS, Brazil
AB - The plant Calendula officinalis L. is widely applied due to its medicinal properties, which
are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical
components in a hydroethanolic extract (HECO) from the leaves of Calendula officinalis L. using
UV-VIS spectrophotometry and thin layer chromatography (TLC), as well as to identify and
quantify the components related to its antioxidant capacity employing high performance liquid
chromatographic (HPLC). The antioxidant capacity evaluation was performed using the DPPH
method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by
UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF). The results
show the plant’s strong antioxidant activity (DPPH and hydroxyl methods), which we believe to
be related to the presence of flavonoids (24.67 mg/g), polyphenols (33.90 mg/g), condensed
tannins (27.30 mg/g), and the amount of rutin (37.25 mg/g), and quercetin (6.09 mg/g) found
during the study. The HECO presented a good antioxidant capacity, most likely due to the
polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05
does not allow the plant to be classified as a stand-alone sunscreen, and more studies are
needed in order to test its ability to enhance sunscreens in existing cosmetic formulations. ©
2015, Instituto de Biociencias. All rights reserved.
KW - Antioxidant
KW - Calendula officinalis
KW - Marigold
KW - Phenolic Compounds
KW - Photo-protection
KW - alkaloid
KW - ascorbic acid
KW - Calendula officinalis extract
KW - essential oil
KW - flavonoid
KW - plant extract
KW - polyphenol
KW - quercetin
KW - rutoside
KW - saponin
KW - sunscreen
KW - superoxide
KW - tannin
KW - terpenoid
KW - unclassified drug
KW - antioxidant activity
KW - Article
KW - Calendula officinalis
KW - concentration (parameters)
KW - controlled study
KW - DPPH radical scavenging assay
KW - drug activity
KW - flow rate
KW - high performance liquid chromatography
KW - hydroxyl radical scavenging assay
KW - IC50
KW - in vitro study
KW - nonhuman
KW - photo protective capacity
KW - phytochemistry
KW - plant leaf
KW - qualitative analysis
KW - thin layer chromatography
KW - ultraviolet spectrophotometry
PB - Instituto de Biociencias
SN - 15160572 (ISSN)
LA - English
J2 - Rev. Bras. Plantas Med.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: V.C.K.N. Deuschle;
Department of Industrial Pharmacy (Phytochemical Research Laboratory), Federal University of
Santa Maria, Center for Health Sciences, Santa Maria, Build 26, ZIP 97105-900, Brazil; email:
vivianenunes1@yahoo.com.br; CODEN: RBPMF
ER -

TY - JOUR
AU - Nikmehr, B.
AU - Ghaznavi, H.
AU - Rahbar, A.
AU - Sadr, S.
AU - Mehrzadi, S.
TI - In vitro anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers,
Datura stramonium seeds, and Salvia officinalis leaves
PY - 2014
T2 - Chinese Journal of Natural Medicines
VL - 12
IS - 6
SP - 423
EP - 427
DO - 10.1016/S1875-5364(14)60066-2
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904644929&doi=10.1016%2fS1875-53
64%2814%2960066-2&partnerID=40&md5=f56e286e3b5d4aba57c27f881f4aeed5
AD - Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran
AD - Cellular and Molecular Research Center, Zahedan University of Medical Sciences,
Zahedan, Iran
AD - Department of Pharmacology, School of Medicine, Iran University of Medical Sciences,
Tehran, Iran
AD - Department of Pathobiology, Veterinary Faculty, Shahid Chamran University, Ahwaz, Iran
AD - Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AD - Core Facilities, Iran University of Medical Sciences, Tehran, Iran
AB - Aim: The anti-leishmanial activity of methanolic extracts of Calendula officinalis flowers,
Datura stramonium seeds, and Salvia officinalis leaves against extracellular (promastigote) and
intracellular (amastigote) forms of Leishmania major were evaluated in this study. Method: In the
first stage, promastigote forms of L. major, were treated with different doses of the plant extracts
in a 96-well tissue-culture microplate and IC50 values for each extract were measured with
colorimetric MTT assay. In the second stage, macrophage cells were infected with L. major
promastigotes. Infected macrophages were treated with plant extracts. Then the macrophages
were stained with Gimsa and the number of infected macrophages and amastigotes were
counted with a light microscope. Results: The results indicated that the plant extracts inhibited
the growth of promastigotes and amastigotes of L. major. Inhibitory concentrations (IC50) for
promastigote assay were 108.19, 155.15, and 184.32 μg7·mL-1 for C. officinalis flowers, D.
stramonium seeds and S. officinalis, respectively. The extracts also reduced the number of
amastigotes in macrophage cells from 264 for control group to 88, 97, and 102 for test groups.
Although the anti-leishmanial activity of the extracts were not comparable with the standard
drug, miltefosine; but they showed significant efficiency in reducing the number of amastigotes
in macrophages, in comparison with the control group (P < 0.001). These plant extracts had
lower toxicity compared with miltefosine. Conclusion: This study demonstrates the potential
efficacy of the methanolic extracts of C. officinalis flowers, D. stramonium seeds, and S.
officinalis leaves to control of cutaneous leishmaniasis. © 2014 China Pharmaceutical
University.
KW - Amastigote
KW - Calendula officinalis
KW - Datura stramonium
KW - Leishmania major
KW - Promastigote
KW - Salvia officinalis
KW - Antiparasitic Agents
KW - Calendula
KW - Cell Line
KW - Datura stramonium
KW - Flowers
KW - In Vitro Techniques
KW - Leishmania major
KW - Leishmaniasis
KW - Macrophages
KW - Phytotherapy
KW - Plant Extracts
KW - Plant Leaves
KW - Salvia officinalis
KW - Seeds
KW - antileishmanial agent
KW - calendula officinalis extract
KW - Datura stramonium extract
KW - miltefosine
KW - plant extract
KW - Salvia officinalis extract
KW - unclassified drug
KW - antiparasitic agent
KW - plant extract
KW - amastigote
KW - antiprotozoal activity
KW - article
KW - Calendula officinalis
KW - controlled study
KW - cytotoxicity
KW - Datura stramonium
KW - in vitro study
KW - Leishmania major
KW - macrophage
KW - nonhuman
KW - promastigote
KW - Salvia officinalis
KW - Calendula
KW - cell line
KW - Datura stramonium
KW - drug effects
KW - flower
KW - leishmaniasis
KW - parasitology
KW - phytotherapy
KW - plant leaf
KW - plant seed
KW - Salvia officinalis
PB - China Pharmaceutical University
SN - 18755364 (ISSN)
C2 - 24969522
LA - English
J2 - Chin. J. Nat. Med.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 31; Correspondence Address: S. Mehrzadi;
Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran,
Iran; email: sa_mehrzadi@yahoo.com; CODEN: ZTYHA
ER -

TY - JOUR
AU - Zaki, A.
AU - Ashour, A.
AU - Mira, A.
AU - Kishikawa, A.
AU - Nakagawa, T.
AU - Zhu, Q.
AU - Shimizu, K.
TI - Biological Activities of Oleanolic Acid Derivatives from Calendula officinalis Seeds
PY - 2016
T2 - Phytotherapy Research
VL - 30
IS - 5
SP - 835
EP - 841
DO - 10.1002/ptr.5589
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84959432334&doi=10.1002%2fptr.5589&
partnerID=40&md5=dfe838ebbb074a09bdc1ea1f74e556a6
AD - Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University,
Fukuoka, Japan
AD - Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura,
Egypt
AD - National Center for Natural Product Research, University of Mississippi, University, 38677,
MS, United States
AB - Phytochemical examination of butanol fraction of Calendula officinalis seeds led to the
isolation of two compounds identified as 28-O-β-D-glucopyranosyl-oleanolic acid
3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS1) and oleanolic acid
3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS2). Biological evaluation was
carried out for these two compounds such as melanin biosynthesis inhibitory, hyaluronic acid
production activities, anti obesity using lipase inhibition and adipocyte differentiation as well as
evaluation of the protective effect against hydrogen peroxide induced neurotoxicity in neuro-2A
cells. The results showed that, compound CS2 has a melanin biosynthesis stimulatory activity;
however, compound CS1 has a potent stimulatory effect for the production of hyaluronic acid on
normal human dermal fibroblast from adult (NHDF-Ad). Both compounds did not show any
inhibitory effect on both lipase and adipocyte differentiation. Compound CS2 could protect
neuro-2A cells and increased cell viability against H2O2. These activities (melanin biosynthesis
stimulatory and protective effect against H2O2 of CS2 and hyaluronic acid productive activities
of these triterpene derivatives) have been reported for the first time. Copyright © 2016 John
Wiley & Sons, Ltd.
KW - anti obesity
KW - Calendula officinalis
KW - hyaluronic acid production
KW - melanin
KW - triterpene
KW - Calendula
KW - Humans
KW - Oleanolic Acid
KW - Plant Extracts
KW - Seeds
KW - 28 o beta dextro glucopyranosyl oleanolic acid
KW - 3 o beta dextro glucopyranosyl glucopyranosiduronic acid
KW - oleanolic acid derivative
KW - tetrahydrolipstatin
KW - unclassified drug
KW - oleanolic acid
KW - plant extract
KW - animal cell
KW - Article
KW - biological activity
KW - Calendula officinalis
KW - cell differentiation
KW - cell protection
KW - controlled study
KW - drug identification
KW - drug isolation
KW - drug mechanism
KW - drug screening
KW - drug structure
KW - melanogenesis
KW - mouse
KW - neuroprotection
KW - neurotoxicity
KW - nonhuman
KW - plant seed
KW - Calendula
KW - chemistry
KW - human
PB - John Wiley and Sons Ltd
SN - 0951418X (ISSN)
C2 - 26887328
LA - English
J2 - Phytother. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 10; Correspondence Address: K. Shimizu;
Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University,
Fukuoka, Japan; email: Japanshimizu@agr.kyushu-u.ac.jp; CODEN: PHYRE
ER -

TY - JOUR
AU - Givol, O.
AU - Kornhaber, R.
AU - Visentin, D.
AU - Cleary, M.
AU - Haik, J.
AU - Harats, M.
TI - A systematic review of Calendula officinalis extract for wound healing
PY - 2019
T2 - Wound Repair and Regeneration
VL - 27
IS - 5
SP - 548
EP - 561
DO - 10.1111/wrr.12737
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067883238&doi=10.1111%2fwrr.12737
&partnerID=40&md5=e454f8f9b4448358a96ad1ba16fb4ece
AD - Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
AD - Department of Plastic and Reconstructive Surgery, Sheba Medical Center, Tel Hashomer,
Israel
AD - College of Health and Medicine, University of Tasmania, Sydney, NSW, Australia
AD - University of Notre Dame Australia, Fremantle, WA, Australia
AD - Talpiot Leadership Program, Sheba Medical Center, Tel Hashomer, Israel
AB - Use of complementary and alternative medicine for wound healing is influencing
mainstream medical practice. This systematic review evaluates the role of Calendula officinalis
flower extract as monotherapy compared to control for wound healing in vivo. Searches were
conducted in PubMed, EMBASE, Cochrane Central Register of Controlled Trials, CINAHL, and
Scopus (up to April 2018) with 14 studies meeting the inclusion criteria, comprising 7 animal
experiments and 7 clinical trials. Findings from the review on acute wound healing showed
faster resolution of the inflammation phase with increased production of granulation tissue in the
test groups treated with extract. These findings were consistent in five animal studies and one
randomized clinical trial. Chronic wound healing studies were varied. Two clinical control studies
on venous ulcers demonstrated decreased ulcer surface area compared to controls. Another
randomized clinical trial demonstrated no improvement for the calendula group in diabetic leg
ulcer healing. Burn healing similarly showed mixed results. Two animal studies demonstrated a
prophylactic effect for the administration of calendula extract prior to burn injury. A randomized
clinical trial of patients suffering from partial to full thickness burns demonstrated no benefit for
topical application of calendula extract compared to controls. Two randomized clinical trials
assessed the potential for extract to prevent acute post radiation dermatitis, with one study
showing improvements compared to trolamine, while the other found no improvement compared
to aqua gel cream. Animal studies provide moderate evidence for improved recovery from the
inflammation phase and increased production of granulation tissue in calendula extract
treatment groups. This review identified some evidence for the beneficial effects of C. officinalis
extract for wound healing, consistent with its role in traditional medicine. There is a need for
larger, well-designed randomized control trials to assess the effect of calendula on wound
healing including complications. © 2019 by the Wound Healing Society
KW - Administration, Topical
KW - Animals
KW - Calendula
KW - Clinical Trials as Topic
KW - Granulation Tissue
KW - Humans
KW - Models, Animal
KW - Ointments
KW - Phytotherapy
KW - Plant Extracts
KW - Treatment Outcome
KW - Varicose Ulcer
KW - Wound Healing
KW - Calendula officinalis extract
KW - flower extract
KW - unclassified drug
KW - wound healing promoting agent
KW - plant extract
KW - burn
KW - Calendula officinalis
KW - cream
KW - evaluation study
KW - gel
KW - granulation tissue
KW - human
KW - in vivo study
KW - inflammation
KW - leg ulcer
KW - monotherapy
KW - nonhuman
KW - priority journal
KW - prophylaxis
KW - radiation dermatitis
KW - Review
KW - skin defect
KW - skin injury
KW - surface area
KW - systematic review
KW - traditional medicine
KW - ulcer
KW - wound
KW - wound healing
KW - animal
KW - animal model
KW - Calendula
KW - chemistry
KW - clinical trial (topic)
KW - drug effect
KW - ointment
KW - pathology
KW - pharmacology
KW - physiology
KW - phytotherapy
KW - topical drug administration
KW - treatment outcome
KW - varicosis
KW - wound healing
PB - Blackwell Publishing Inc.
SN - 10671927 (ISSN)
C2 - 31145533
LA - English
J2 - Wound Repair Regen.
M3 - Review
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 64; Correspondence Address: O. Givol; Sackler
School of Medicine, Tel Aviv University, Tel Aviv, Israel; email: orgivol@gmail.com; CODEN:
WRERE
ER -

TY - JOUR
AU - Raal, A.
AU - Orav, A.
AU - Nesterovitsch, J.
AU - Maidla, K.
TI - Analysis of carotenoids, flavonoids and essential oil of calendula officinalis cultivars
growing in Estonia
PY - 2016
T2 - Natural Product Communications
VL - 11
IS - 8
SP - 1157
EP - 1160
DO - 10.1177/1934578x1601100831
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019071414&doi=10.1177%2f1934578x
1601100831&partnerID=40&md5=a39befdfe81b0858f74e97e533d803cb
AD - Institute of Pharmacy, University of Tartu, Tartu, 50411, Estonia
AB - Pot Marigold (Calendula officinalis L.) is an annual ornamental plant with yellow to orange
flowers which belongs the the Asteraceae family, its flowers are used in the pharmaceutical,
cosmetic and food industries. The commercial samples of seeds (n=24) of C. officinalis were
cultivated in home gardens in Estonia. Total carotenoid contents, determined
spectrophotometrically (=455 nm) in the inflorescences, varied from 0.7 to 2.7%, the cultivar
with the highest carotenoid content was 'Dracon'. The total content of flavonoids, determined
spectrophotometrically (=370 nm), varied from 0.8% to 1.7%. The best cultivar contained almost
maximum amount of total carotenoids (2.5%) and flavonoids (1.7%) was 'Radio. The oil yields of
eight cultivars of C. officinalis obtained were from 0.10 to 0.43%, the highest yield (0.43%) of the
oil was obtained from the cultivar 'Double Ball'. A total 70 compounds were identified and
analysed by GC-MS in the essential oil of C. officinalis, representing over 95% from total oil. The
major components of the oils (>5%) of C. officinalis cultivars were -cadinole (18.4-32.0%),
cadinene (9.7-18.9%), viridiflorol (2.0-10.0%), cadinole (4.7-7.9%), cadinole (3.3-5.6%), and
muurolene (0.8-5.1%). There is no clear relation between the colour of the inflorescences of C.
officinalis and the total content of flavonoids and carotenoids. The chemotypes of essential oils
of C. officinalis were not found among the cultivars studied.
KW - Calendula officinalis
KW - Carotenoids
KW - Chemotypes
KW - Essential oil
KW - Estonia
KW - Flavonoids
KW - Pot Marigold
KW - alpha cadinole
KW - Calendula officinalis extract
KW - carotenoid
KW - delta cadinole
KW - essential oil
KW - flavonoid
KW - gamma cadinene
KW - gamma muurolene
KW - plant extract
KW - plant medicinal product
KW - tau cadinole
KW - unclassified drug
KW - viridiflorol
KW - Article
KW - Calendula officinalis
KW - Estonia
KW - mass fragmentography
KW - plant seed
KW - spectrophotometry
PB - Natural Product Incorporation
SN - 1934578X (ISSN)
LA - English
J2 - Nat. Pro. Comm.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 10; Correspondence Address: A. Raal; Institute
of Pharmacy, University of Tartu, Tartu, 50411, Estonia; email: ain.raal@ut.ee
ER -

TY - JOUR
AU - Tanideh, N.
AU - Tavakoli, P.
AU - Saghiri, M.A.
AU - Garcia-Godoy, F.
AU - Amanat, D.
AU - Tadbir, A.A.
AU - Samani, S.M.
AU - Tamadon, A.
TI - Healing acceleration in hamsters of oral mucositis induced by 5-fluorouracil with topical
Calendula officinalis
PY - 2013
T2 - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
VL - 115
IS - 3
SP - 332
EP - 338
DO - 10.1016/j.oooo.2012.08.450
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875382684&doi=10.1016%2fj.oooo.20
12.08.450&partnerID=40&md5=39e845d56bb111c18787e0b45ebd6a70
AD - Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences,
Shiraz, Iran
AD - Dental Clinic, Shiraz, Iran
AD - Department of Dental Materials, School of Dentistry, Islamic Azad University, Tehran, Iran
AD - Bioscience Research Center, College of Dentistry, University of Tennessee Health Science
Center, Memphis, TN, United States
AD - Department of Oral Medicine, School of Dental Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
AD - Department of Oral Pathology, School of Dentistry, Shiraz University of Medical Sciences,
Shiraz, Iran
AD - Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Shiraz University of
Medical Sciences, Shiraz, Iran
AD - Department of Animal Health Management, School of Veterinary Medicine, Shiraz
University, Iran
AD - Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical
Sciences, Shiraz, Iran
AB - Objective: This study assessed the potential of topical Calendula officinalis extract on the
healing of oral mucositis induced by 5-fluorouracil (5-FU) in hamsters. Study Design: Oral
mucositis was induced in 60 male hamsters by 5-FU (60 mg/kg) on days 0, 5, and 10 of the
study. The cheek pouch was scratched with a sterile needle on days 1 and 2. On days 12-17,
5% and 10% C. officinalis gel and gel base groups were treated and then compared with a
control group. Macroscopic and microscopic scores and weights were evaluated. Results:
Microscopic and macroscopic scores of mucositis were lower in the 5% and 10% C. officinalis
gel groups than in the gel base and control groups (P <.05). Weight gain was noted in the
treatment groups compared with the gel base and control groups (P <.05). Conclusions:
Calendula officinalis extract accelerated the healing of oral mucositis in hamsters. © 2013
Elsevier Inc. All rights reserved.
KW - Abscess
KW - Animals
KW - Antimetabolites, Antineoplastic
KW - Calendula
KW - Carboxymethylcellulose Sodium
KW - Cricetinae
KW - Drug Carriers
KW - Erythema
KW - Fluorouracil
KW - Gels
KW - Hyperemia
KW - Injections, Intraperitoneal
KW - Male
KW - Mesocricetus
KW - Mouth Diseases
KW - Mouth Mucosa
KW - Oral Hemorrhage
KW - Oral Ulcer
KW - Phytotherapy
KW - Plant Extracts
KW - Re-Epithelialization
KW - Single-Blind Method
KW - Stomatitis
KW - Time Factors
KW - Weight Gain
KW - Wound Healing
KW - antineoplastic antimetabolite
KW - carboxymethylcellulose
KW - drug carrier
KW - fluorouracil
KW - plant extract
KW - abscess
KW - animal
KW - article
KW - Calendula
KW - chemically induced disorder
KW - comparative study
KW - drug effect
KW - epithelization
KW - erythema
KW - gel
KW - hamster
KW - hyperemia
KW - injury
KW - intraperitoneal drug administration
KW - male
KW - methodology
KW - mouth disease
KW - mouth mucosa
KW - mouth ulcer
KW - oral bleeding
KW - pathology
KW - phytotherapy
KW - single blind procedure
KW - stomatitis
KW - Syrian hamster
KW - time
KW - weight gain
KW - wound healing
SN - 22124403 (ISSN)
C2 - 23182376
LA - English
J2 - Oral Surg. Oral Med. Oral Pathol. Oral Radiol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 37; Correspondence Address: M.A. Saghiri;
Department of Dental Materials, School of Dentistry, Islamic Azad University, Tehran, Iran;
email: saghiri@gmail.com
ER -

TY - JOUR
AU - Siddiquee, S.
AU - McGee, M.A.
AU - Vincent, A.D.
AU - Giles, E.
AU - Clothier, R.
AU - Carruthers, S.
AU - Penniment, M.
TI - Efficacy of topical Calendula officinalis on prevalence of radiation-induced dermatitis: A
randomised controlled trial
PY - 2021
T2 - Australasian Journal of Dermatology
VL - 62
IS - 1
SP - e35
EP - e40
DO - 10.1111/ajd.13434
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091310038&doi=10.1111%2fajd.13434
&partnerID=40&md5=22863089be8a8e3d15ee14624ed3dc44
AD - Adelaide Medical School, University of Adelaide, Adelaide, Australia
AD - Freemasons Foundation Centre for Men’s Health, University of Adelaide, Adelaide,
Australia
AD - Department of Allied Health and Human Performance, University of South Australia,
Adelaide, Australia
AD - Department of Radiation Oncology, Royal Adelaide Hospital, Adelaide, Australia
AB - Objectives: A randomised controlled trial was undertaken to compare the efficacy of
topical Calendula officinalis (Calendula) versus standard of care (Sorbolene: 10% glycerine in
cetomacragol cream) in reducing the prevalence of radiation-induced dermatitis in women
undergoing breast cancer radiotherapy. Methods: A total of 271 women were screened and 82
were randomised. The primary outcome was prevalence of acute radiation-induced dermatitis
(RTOG grade 2+) assessed at multiple skin sites. A chi-squared test was conducted for the
primary outcome with a worst-case scenario imputation. Results: The recruitment target
(n = 178) was not achieved. A total of n = 81 participants were analysed (n = 40 Calendula;
n = 41 Sorbolene). There was no detectable difference in prevalence of radiation-induced
dermatitis grade 2+ between the Calendula (53%) and Sorbolene (62%) groups (primary
analysis OR = 0.87, 95% CI: [0.36, 2.09], P = 0.92; covariate adjusted complete case analysis
OR 0.40, 95% CI: [0.13, 1.20], P = 0.10). Conclusion: This randomised controlled trial showed
no difference between Calendula and standard of care (Sorbolene) for the prevention of
radiation-induced dermatitis. However, the study was underpowered (limited recruitment) for the
primary comparison. © 2020 The Australasian College of Dermatologists
KW - adjuvant
KW - calendula
KW - ointments/therapeutic
KW - radiodermatitis
KW - radiotherapy
KW - randomised controlled trial
KW - Administration, Topical
KW - Adult
KW - Aged
KW - Breast Neoplasms
KW - Calendula
KW - Female
KW - Humans
KW - Middle Aged
KW - Ointments
KW - Phytotherapy
KW - Plant Extracts
KW - Radiodermatitis
KW - Single-Blind Method
KW - antineoplastic agent
KW - Calendula officinalis extract
KW - dermatological agent
KW - plant extract
KW - sorbolene
KW - unclassified drug
KW - plant extract
KW - adult
KW - aged
KW - Article
KW - breast cancer
KW - burning sensation
KW - Calendula officinalis
KW - cancer adjuvant therapy
KW - cancer radiotherapy
KW - chemoprophylaxis
KW - controlled study
KW - desquamation
KW - drug efficacy
KW - female
KW - human
KW - lumpectomy
KW - major clinical study
KW - mastectomy
KW - pain
KW - parallel design
KW - prevalence
KW - pruritus
KW - radiation dermatitis
KW - radiation dose
KW - randomized controlled trial
KW - single blind procedure
KW - skin discomfort
KW - skin necrosis
KW - treatment outcome
KW - breast tumor
KW - Calendula
KW - middle aged
KW - ointment
KW - phytotherapy
KW - radiation dermatitis
KW - topical drug administration
PB - Blackwell Publishing
SN - 00048380 (ISSN)
C2 - 32965030
LA - English
J2 - Australas. J. Dermatol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 14; Correspondence Address: S. Siddiquee;
Adelaide Medical School, University of Adelaide, Adelaide, Australia; email:
shihabsiddiquee@gmail.com; CODEN: AJDEB
ER -

TY - JOUR
AU - Verma, P.K.
AU - Raina, R.
AU - Sultana, M.
AU - Singh, M.
AU - Kumar, P.
TI - Total antioxidant and oxidant status of plasma and renal tissue of cisplatin-induced
nephrotoxic rats: Protection by floral extracts of Calendula officinalis Linn
PY - 2016
T2 - Renal Failure
VL - 38
IS - 1
SP - 142
EP - 150
DO - 10.3109/0886022X.2015.1103585
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955650209&doi=10.3109%2f0886022
X.2015.1103585&partnerID=40&md5=f49dc7a71e7f8ce50b0993033f53a0aa
AD - Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Sciences and
Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology -
Jammu, RS Pura, Jammu, Jammu and Kashmir, 181102, India
AD - Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Sciences and
Animal Husbandry, RS Pura, Jammu, Jammu and Kashmir, India
AD - Division of Veterinary Public Health and Epidemiology, Faculty of Veterinary Sciences and
Animal Husbandry, RS Pura, Jammu, Jammu and Kashmir, India
AD - Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India
AB - The present study was aimed to determine the total antioxidant status (TAS), total oxidant
status (TOS) and oxidative stress index (OSI) of plasma and renal tissue in cisplatin (cDDP)
induced nephrotoxic rats and its protection by treatments with floral extracts of Calendula
officinalis Linn. Treatment with cDDP elevated (p < 0.05) the levels of blood urea nitrogen,
creatinine (CR), TOS, OSI and malondialdehyde (MDA) but lowered (p < 0.05) total plasma
proteins, TAS, total thiols (TTH), blood glutathione (GSH) and antioxidant enzymes compared to
the control group. Pre- and post-treatments of ethanolic floral extract of C. officinalis along with
cDDP restored (p > 0.05) CR, albumin, TOS, GSH and activities of antioxidant enzymes in
blood and renal tissue. Ethanolic extract treatments reduced (p < 0.05) MDA level in renal tissue
without restoring the erythrocyte MDA level following cDDP treatment. These observations were
further supported by the histopathological findings in renal tissue. Observations of the present
study have shown that treatments with ethanolic floral extract of C. officinalis protect cDDP
induced nephrotoxicity by restoring antioxidant system of the renal tissue. © 2015 Taylor &
Francis.
KW - Calendula officinalis
KW - cisplatin
KW - nephrotoxicity
KW - oxidative stress index
KW - total antioxidant status
KW - Animals
KW - Antineoplastic Agents
KW - Antioxidants
KW - Calendula
KW - Cisplatin
KW - Drug Evaluation, Preclinical
KW - Female
KW - Kidney
KW - Kidney Diseases
KW - Male
KW - Phytotherapy
KW - Plant Extracts
KW - Random Allocation
KW - Rats, Wistar
KW - alcohol
KW - antioxidant
KW - Calendula extract
KW - Calendula officinalis extract
KW - catalase
KW - cisplatin
KW - creatinine
KW - globulin
KW - glutathione
KW - glutathione peroxidase
KW - glutathione transferase
KW - malonaldehyde
KW - plasma protein
KW - serum albumin
KW - thiol derivative
KW - unclassified drug
KW - uric acid
KW - antineoplastic agent
KW - antioxidant
KW - cisplatin
KW - plant extract
KW - albumin blood level
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - Calendula officinalis
KW - controlled study
KW - creatinine blood level
KW - enzyme activity
KW - erythrocyte
KW - female
KW - flower
KW - globulin blood level
KW - histopathology
KW - kidney parenchyma
KW - male
KW - nephrotoxicity
KW - nonhuman
KW - oxidative stress
KW - priority journal
KW - protein blood level
KW - rat
KW - renal protection
KW - urea nitrogen blood level
KW - uric acid blood level
KW - animal
KW - blood
KW - Calendula
KW - chemically induced
KW - drug effects
KW - kidney
KW - Kidney Diseases
KW - metabolism
KW - phytotherapy
KW - preclinical study
KW - randomization
KW - Wistar rat
PB - Taylor and Francis Ltd
SN - 0886022X (ISSN)
C2 - 26513373
LA - English
J2 - Renal Fail.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 30; Correspondence Address: P.K. Verma;
Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Sciences and Animal
Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology - Jammu, RS
Pura, Jammu, Jammu and Kashmir, 181102, India; email: drpawankv@yahoo.co.in; CODEN:
REFAE
ER -

TY - JOUR
AU - Kadowaki, W.
AU - Miyata, R.
AU - Fujinami, M.
AU - Sato, Y.
AU - Kumazawa, S.
TI - Catechol-O-methyltransferase Inhibitors from Calendula officinalis Leaf
PY - 2023
T2 - Molecules
VL - 28
IS - 3
C7 - 1333
DO - 10.3390/molecules28031333
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147895079&doi=10.3390%2fmolecule
s28031333&partnerID=40&md5=b0ff498df60525c32a094a634365edc9
AD - Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of
Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan
AD - Kisho Corporation Co., Ltd, 3-4-27 Kitasuna, Tokyo, Koto-ku, 136-0073, Japan
AB - Calendula officinalis is commonly known as marigold and its flowers are used in herbal
medicines, cosmetics, perfumes, dyes, pharmaceutical preparations, and food products.
However, the utility of its leaves has not been studied in depth. The purpose of the present
study was to identify the major compounds in C. officinalis leaves and to determine the inhibitory
properties of the isolated compounds toward human catechol-O-methyltransferase (COMT), a
key neurotransmitter involved in Parkinson’s disease and depression. We isolated and identified
ten compounds, including two phenylpropanoids and seven flavonoids, from C. officinalis leaf
extracts, of which four flavonoids were identified from C. officinalis leaves for the first time. Eight
compounds exhibited COMT inhibitory activities with IC50 values of less than 100 μM. Our
results indicate that compounds in C. officinalis leaves are potentially effective for preventing
Parkinson’s disease and depression. Thus, C. officinalis leaves may hold promise as dietary
supplements. © 2023 by the authors.
KW - Calendula officinalis
KW - catechol-O-methyltransferase
KW - flavonoid
KW - Parkinson’s disease
KW - phenylpropanoid
KW - Calendula
KW - Catechol O-Methyltransferase
KW - Catechol O-Methyltransferase Inhibitors
KW - Flavonoids
KW - Humans
KW - Parkinson Disease
KW - Plant Extracts
KW - catechol methyltransferase
KW - catechol methyltransferase inhibitor
KW - flavonoid
KW - plant extract
KW - Calendula
KW - human
KW - Parkinson disease
PB - MDPI
SN - 14203049 (ISSN)
C2 - 36770999
LA - English
J2 - Molecules
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: S. Kumazawa;
Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka,
Shizuoka, 52-1 Yada, Suruga-ku, 422-8526, Japan; email: kumazawa@u-shizuoka-ken.ac.jp;
CODEN: MOLEF
ER -

TY - JOUR
AU - Kaur, J.
AU - Sidhu, S.
AU - Chopra, K.
AU - Khan, M.U.
TI - Calendula officinalis ameliorates l-arginine-induced acute necrotizing pancreatitis in rats
PY - 2016
T2 - Pharmaceutical Biology
VL - 54
IS - 12
SP - 2951
EP - 2959
DO - 10.1080/13880209.2016.1195848
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84976315591&doi=10.1080%2f13880209
.2016.1195848&partnerID=40&md5=fd853719787df95606b331bf68c6f7dd
AD - Department of Research Innovations and Consultancy, IKG Punjab Technical University,
Kapurthala, Punjab, India
AD - Sri Sai College of Pharmacy, Badhani, Pathankot, Punjab, India
AD - Department of Life Sciences and Technology, Punjab Institute of Technology, IKG Punjab
Technical University, Kapurthala, Punjab, India
AD - Pharmacology Research Laboratory, University Institute of Pharmaceutical Sciences,
UGC Centre of Advanced Study, Panjab University, Chandigarh, India
AB - Context:Calendula officinalis L. (Asteraceae) has been traditionally used in treating
inflammation of internal organs, gastrointestinal tract ulcers and wound healing. Objective: The
present study investigates the effect of ethanol extract (95%) of Calendula officinalis flowers in
l-arginine induced acute necrotizing pancreatitis in rats. Materials and methods: Rats were
divided into four groups: normal control, l-arginine control, Calendula officinalis extract (COE)
treated and melatonin treated (positive control), which were further divided into subgroups (24 h,
day 3 and 14) according to time points. Two injections of l-arginine 2 g/kg i.p. at 1 h intervals
were administered in l-arginine control, COE and melatonin-treated groups to produce acute
necrotizing pancreatitis. Biochemical parameters [serum amylase, lipase, pancreatic amylase,
nucleic acid content, total proteins, transforming growth factor-β1 (TGF-β1), collagen content,
lipid peroxidation, reduced glutathione and nitrite/nitrate] and histopathological studies were
carried out. Results: COE treatment (400 mg/kg p.o.) was found to be beneficial. This was
evidenced by significantly lowered histopathological scores (2 at day 14). Nucleic acid content
(DNA 21.1 and RNA 5.44 mg/g pancreas), total proteins (0.66 mg/mL pancreas) and pancreatic
amylase (1031.3 100 SU/g pancreas) were significantly improved. Marked reduction in
pancreatic oxidative and nitrosative stress; collagen (122 μmoles/100 mg pancreas) and
TGF-β1 (118.56 pg/mL) levels were noted. Results obtained were comparable to those of
positive control. Discussion and conclusion: The beneficial effect of COE may be attributed to its
antioxidant, antinitrosative and antifibrotic actions. Hence, the study concludes that COE
promotes spontaneous repair and regeneration of the pancreas. © 2016 Informa UK Limited,
trading as Taylor & Francis Group.
KW - Melatonin
KW - oxidative and nitrosative stress
KW - regeneration
KW - TGF-β1
KW - Animals
KW - Arginine
KW - Calendula
KW - Female
KW - Male
KW - Pancreatitis, Acute Necrotizing
KW - Plant Extracts
KW - Rats
KW - Rats, Sprague-Dawley
KW - alcohol
KW - amylase
KW - antioxidant
KW - arginine
KW - Calendula officinalis extract
KW - collagen
KW - DNA
KW - glutathione
KW - melatonin
KW - nitrate
KW - nitrite
KW - nucleic acid
KW - plant extract
KW - RNA
KW - transforming growth factor beta1
KW - triacylglycerol lipase
KW - unclassified drug
KW - arginine
KW - plant extract
KW - acute hemorrhagic pancreatitis
KW - amylase blood level
KW - animal experiment
KW - animal model
KW - Article
KW - Calendula officinalis
KW - controlled study
KW - flower
KW - histopathology
KW - lipid peroxidation
KW - nitrosative stress
KW - nonhuman
KW - oxidative stress
KW - pancreas level
KW - protein blood level
KW - rat
KW - tissue level
KW - triacylglycerol lipase blood level
KW - acute hemorrhagic pancreatitis
KW - animal
KW - Calendula
KW - chemically induced
KW - female
KW - isolation and purification
KW - male
KW - metabolism
KW - Sprague Dawley rat
PB - Taylor and Francis Ltd
SN - 13880209 (ISSN)
C2 - 27339751
LA - English
J2 - Pharm. Biol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: M.U. Khan; Sri Sai
College of Pharmacy, Badhani, Pathankot, 145 001, India; email: masihkhan@gmail.com;
CODEN: PHBIF
ER -

TY - JOUR
AU - Dinda, M.
AU - Dasgupta, U.
AU - Singh, N.
AU - Bhattacharyya, D.
AU - Karmakar, P.
TI - PI3K-Mediated proliferation of fibroblasts by calendula officinalis tincture: Implication in
wound healing
PY - 2015
T2 - Phytotherapy Research
VL - 29
IS - 4
SP - 607
EP - 616
DO - 10.1002/ptr.5293
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926460734&doi=10.1002%2fptr.5293&
partnerID=40&md5=82ace1ffb6626eb099e0e8fd0da56dd2
AD - Department of Life Science and Biotechnology, Jadavpur University West Bengal, 188,
Raja S.C. Mullick Road, Kolkata, 700 032, India
AD - Division of Structural Biology and Bio-Informatics, Indian Institute of Chemical Biology
West Bengal, 4, Raja S.C. Mullick Road, Kolkata, 700 032, India
AB - Calendula officinalis, a member of the Asteraceae family, is a flowering plant and has been
used for its antibacterial, antifungal, antiviral, antiinflammatory, anticancer and wound healing
activity. The mode of action of C. officinalis tincture on wound healing is poorly understood.
Here, we investigated the role of C. officinalis tincture (CDOT) on cell viability and wound
closure. C. officinalis tincture stimulated both proliferation and migration of fibroblasts in a
statistically significant manner in a PI3K-dependent pathway. The increase in phosphorylation of
FAK (Tyr 397) and Akt (Ser 473) was detected after treatment of CDOT. Inhibition of the PI3K
pathway by wortmannin and LY294002 decreased both cell proliferation and cell migration.
HPLC-ESI MS revealed the presence of flavonol glycosides as the major compounds of CDOT.
Altogether, our results showed that CDOT potentiated wound healing by stimulating proliferation
and migration of fibroblast in a PI3K-dependent pathway, and the identified compounds are
likely to be responsible for wound healing activity. Copyright © 2015 John Wiley & Sons, Ltd.
Copyright © 2015 John Wiley & Sons, Ltd.
KW - Akt
KW - Calendula officinalis tincture
KW - FAK
KW - fibroblasts
KW - PI3K
KW - Androstadienes
KW - Animals
KW - Calendula
KW - Cell Line
KW - Cell Movement
KW - Cell Proliferation
KW - Chromones
KW - Fibroblasts
KW - Flavonols
KW - Glycosides
KW - Humans
KW - Mice
KW - Morpholines
KW - Phosphatidylinositol 3-Kinases
KW - Phosphorylation
KW - Plant Extracts
KW - Wound Healing
KW - Asteraceae
KW - Calendula officinalis
KW - Magnoliophyta
KW - 2 morpholino 8 phenylchromone
KW - flavonoid glycoside
KW - focal adhesion kinase
KW - herbaceous agent
KW - phosphatidylinositol 3 kinase
KW - protein kinase B
KW - wortmannin
KW - 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
KW - androstane derivative
KW - chromone derivative
KW - flavonol derivative
KW - glycoside
KW - morpholine derivative
KW - phosphatidylinositol 3 kinase
KW - plant extract
KW - animal cell
KW - Article
KW - Calendula officinalis
KW - cell migration
KW - cell proliferation
KW - cell viability
KW - controlled study
KW - drug determination
KW - electrospray mass spectrometry
KW - enzyme activation
KW - female
KW - fibroblast
KW - high performance liquid chromatography
KW - human
KW - human cell
KW - mouse
KW - nonhuman
KW - protein expression
KW - protein phosphorylation
KW - signal transduction
KW - tincture
KW - wound closure
KW - wound healing
KW - animal
KW - Calendula
KW - cell line
KW - cell motion
KW - cell proliferation
KW - chemistry
KW - cytology
KW - drug effects
KW - fibroblast
KW - metabolism
KW - phosphorylation
KW - wound healing
PB - John Wiley and Sons Ltd
SN - 0951418X (ISSN)
C2 - 25641010
LA - English
J2 - Phytother. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 40; Correspondence Address: P. Karmakar;
Department of Life Science and Biotechnology, Jadavpur University West Bengal, Kolkata, 188,
Raja S.C. Mullick Road, 700 032, India; email: pkarmakar_28@yahoo.co.in; CODEN: PHYRE
ER -

TY - JOUR
AU - Muñoz, J.A.M.
AU - Machado, J.E.M.
AU - González, M.T.
TI - Validation of an hplc method for quantification of total quercetin in calendula officinalis
extracts
ST - Validación de una metodología por HPLC para cuantificar quercetina total en extractos de
Calendula officinalis
PY - 2015
T2 - Revista Cubana de Farmacia
VL - 49
IS - 1
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954492546&partnerID=40&md5=7898
5de232356c5b3aec605b1d08b6f6
AD - Universidad Nacional de Colombia, sede Bogotá, D. C, Colombia
AD - Grupo Aseguramiento de Calidad, Departamento de Farmacia, Universidad Nacional de
Colombia, sede Bogotá, D. C, Colombia
AB - Introduction: calendula officinalis extracts are used as natural raw material in a wide range
of pharmaceutical and cosmetic preparations; however, there are no official methods for quality
control of these extracts. Objective: to validate an HPLC-based analytical method for
quantification total quercetin in glycolic and hydroalcoholic extracts of Calendula officinalis.
Methods: to quantify total quercetin content in the matrices, it was necessary to hydrolyze
flavonoid glycosides under optimal conditions. The chromatographic separation was performed
on a C-18 SiliaChrom 4.6x150 mm 5 µm column, adapted to a SiliaChrom 5 um C-18 4.6x10
mm precolumn, with UV detection at 370 nm. The gradient elution was performed with a mobile
phase consisting of methanol (MeOH) and phosphoric acid (H3PO4) (0.08 % w/v). The
quantification was performed through the external standard method and comparison with
quercetin reference standard. Results: the studied method selectivity against extract
components and degradation products under acid/basic hydrolysis, oxidation and light exposure
conditions showed no signals that interfere with the quercetin quantification. It was statistically
proved that the method is linear from 1.0 to 5.0 mg/mL. Intermediate precision expressed as a
variation coefficient was 1.8 and 1.74 % and the recovery percentage was 102.15 and 101.32
%, for glycolic and hydroalcoholic extracts, respectively. Conclusions: the suggested
methodology meets the quality parameters required for quantifying total quercetin, which makes
it a useful tool for quality control of C. officinalis extracts © 2015, Editorial Ciencias Medicas. All
rights reserved.
KW - Calendula officinalis
KW - Flavonoid glycoside
KW - HPLC
KW - Quality control
KW - Quercetin
KW - Validation
KW - alcohol
KW - Calendula officinalis extract
KW - plant extract
KW - quercetin
KW - unclassified drug
KW - Article
KW - Calendula officinalis
KW - controlled study
KW - high performance liquid chromatography
KW - nonhuman
KW - phytochemistry
KW - validation process
PB - Editorial Ciencias Medicas
SN - 00347515 (ISSN)
LA - Spanish
J2 - Rev. Cuba. Farm.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: J.A.M. Muñoz;
Universidad Nacional de Colombia, sede Bogotá, D. C, Colombia; email:
joamunozmu@unal.edu.co; CODEN: RCUFA
ER -

TY - JOUR
AU - Król, B.
AU - Paszko, T.
AU - Król, A.
TI - Conjugated linolenic acid content in seeds of some pot marigold (Calendula officinalis L.)
cultivars grown in Poland
PY - 2016
T2 - Farmacia
VL - 64
IS - 6
SP - 881
EP - 886
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007087171&partnerID=40&md5=d464
3f20fbaf7faf83541ca212dfbe5e
AD - University of Life Sciences, Faculty of Agrobioengineering, Department of Industrial and
Medicinal Plants, Akademicka 15, Lublin, 20-950, Poland
AD - University of Life Sciences, Faculty of Food Science and Biotechnology, Department of
Chemistry, Akademicka 15, Lublin, 20-950, Poland
AD - University of Medical Sciences, Department of Biochemistry and Molecular Biology,
Poznań 6 Święcickiego St., Poznań, 60-781, Poland
AB - Seed oil of ten cultivars of pot marigold grown in Poland was analyzed in order to
determine the main factors affecting its content and composition. The seeds of the pot marigold
cultivars grown in Poland contained relatively large amounts of oil (17.32% ± 2.60%), which was
abundant in conjugated linolenic acids (49.53% ± 5.43%) and had low content of saturated fatty
acids (8.62% ± 1.97%). In addition to the effect of cultivars, the large contents of α-calendic acid
in oils were a result of low average temperature and extensive precipitation during seed
maturation. The statistical analysis suggested that linoleic acid was the main precursor of
α-calendic acid, and α-calendic acid was the precursor of β-calendic acid. © 2016 Romanian
Society for Pharmaceutical Sciences. All rights reserved.
KW - Fatty acid
KW - Oil
KW - Pot marigold
KW - α-calendic acid
KW - β-calendic acid
KW - alpha calendic acid
KW - beta calendic acid
KW - Calendula officinalis extract
KW - linoleic acid
KW - linolenic acid
KW - plant extract
KW - unclassified drug
KW - Article
KW - climate
KW - controlled study
KW - correlation analysis
KW - cultivar
KW - enzyme activity
KW - genotype phenotype correlation
KW - marigold
KW - nonhuman
KW - plant seed
KW - Poland
KW - statistical analysis
KW - temperature
PB - Romanian Society for Pharmaceutical Sciences
SN - 00148237 (ISSN)
LA - English
J2 - Farmacia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 5; Correspondence Address: B. Król; University
of Life Sciences, Faculty of Agrobioengineering, Department of Industrial and Medicinal Plants,
Lublin, Akademicka 15, 20-950, Poland; email: beata.krol@up.lublin.pl; CODEN: FRMBA
ER -

TY - JOUR
AU - Balciunaitiene, A.
AU - Puzeryte, V.
AU - Radenkovs, V.
AU - Krasnova, I.
AU - Memvanga, P.B.
AU - Viskelis, P.
AU - Streimikyte, P.
AU - Viskelis, J.
TI - Sustainable–Green Synthesis of Silver Nanoparticles Using Aqueous Hyssopus officinalis
and Calendula officinalis Extracts and Their Antioxidant and Antibacterial Activities
PY - 2022
T2 - Molecules
VL - 27
IS - 22
C7 - 7700
DO - 10.3390/molecules27227700
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85142940469&doi=10.3390%2fmolecule
s27227700&partnerID=40&md5=733f94eb043f86b2b16f653a37ccc4df
AD - Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Kaunas,
54333, Lithuania
AD - Processing and Biochemistry Department, Institute of Horticulture, Dobele, LV-3701,
Latvia
AD - Research Laboratory of Biotechnology, Division of Smart Technologies, Latvia University
of Life Sciences and Technologies, Jelgava, LV-3004, Latvia
AD - Laboratory of Pharmaceutics and Phytopharmaceutical Drug Development, Faculty of
Pharmaceutical Sciences, University of Kinshasa, B.P. 212, Kinshasa, 012, Congo
AD - Department of Pharmacy, Faculty of Medecine and Pharmacy, University of Kisangani,
B.P. 212, Kisangani, 012, Congo
AD - Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official
University of Bukavu, B.P. 570, Bukavu, Congo
AD - Centre de Recherche et d’Innovation Technologique en Environnement et en Sciences de
la Santé (CRITESS), University of Kinshasa, B.P. 212, Kinshasa, 012, Congo
AB - Silver nanoparticles (AgNPs) biosynthesized using aqueous medical plant extracts as
reducing and capping agents show multiple applicability for bacterial problems. The aim of this
study was to expand the boundaries on AgNPs using a novel, low-toxicity, and cost-effective
alternative and green approach to the biosynthesis of metallic NPs using Calendula officinalis
(Calendula) and Hyssopus officinalis (Hyssopus) aqueous extracts. The formation of AgNPs
was confirmed by transmission electron microscopy (TEM), scanning electron microscopy
(SEM), and energy-dispersive spectroscopy (EDS) techniques. The effectiveness of
biosynthesized AgNPs in quenching free radicals and inhibiting the growth of Gram-positive and
Gram-negative microorganisms was supported by in vitro antioxidant activity assay methods
and using the Kirby–Bauer disk diffusion susceptibility test, respectively. The elucidated
antimicrobial and antioxidative activities of medical plant extracts were compared with data from
the engineered biosynthetic AgNPs. The antimicrobial effect of engineered AgNPs against
selected test cultures was found to be substantially stronger than for plant extracts used for their
synthesis. The analysis of AgNPs by TEM revealed the presence of spherical-shaped
nano-objects. The size distribution of AgNPs was found to be plant-type-dependent. The smaller
AgNPs were obtained with Hyssopus extract (with a size range of 16.8 ± 5.8 nm compared to
35.7 ± 4.8 nm from Calendula AgNPs). The AgNPs’ presumably inherited biological functions of
Hyssopus and Calendula medical plants can provide a platform to combat pathogenic bacteria
in the era of multi-drug resistance. © 2022 by the authors.
KW - antibacterial activity
KW - antioxidant activity
KW - Calendula officinalis
KW - green synthesis
KW - Hyssopus officinalis
KW - phytochemical analysis
KW - silver nanoparticles
KW - Anti-Bacterial Agents
KW - Antioxidants
KW - Bacteria
KW - Calendula
KW - Hyssopus Plant
KW - Metal Nanoparticles
KW - Plant Extracts
KW - Silver
KW - antiinfective agent
KW - antioxidant
KW - metal nanoparticle
KW - plant extract
KW - silver
KW - bacterium
KW - Calendula
KW - chemistry
KW - Hyssopus plant
PB - MDPI
SN - 14203049 (ISSN)
C2 - 36431804
LA - English
J2 - Molecules
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: A. Balciunaitiene;
Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Kaunas,
54333, Lithuania; email: aiste.balciunaitiene@lammc.lt; P. Viskelis; Lithuanian Research Centre
for Agriculture and Forestry, Institute of Horticulture, Kaunas, 54333, Lithuania; email:
pranas.viskelis@lammc.lt; CODEN: MOLEF
ER -

TY - JOUR
AU - Silva, D.
AU - Ferreira, M.S.
AU - Sousa-Lobo, J.M.
AU - Cruz, M.T.
AU - Almeida, I.F.
TI - Anti-inflammatory activity of calendula officinalis l. Flower extract
PY - 2021
T2 - Cosmetics
VL - 8
IS - 2
C7 - 31
DO - 10.3390/cosmetics8020031
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107888654&doi=10.3390%2fcosmetics
8020031&partnerID=40&md5=336a5d97d0f5e861ffb4c757d1b9f319
AD - Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of
Pharmacy, University of Porto, Porto, 4050-313, Portugal
AD - UCIBIO/REQUIMTE, MedTech, Laboratory of Pharmaceutical Technology, Department of
Drug Sciences, Faculty of Pharmacy, University of Porto, Porto, 4050-313, Portugal
AD - Faculty of Pharmacy, University of Coimbra, Coimbra, 3004-531, Portugal
AD - Center for Neurosciences and Cell Biology, Coimbra, 3004-504, Portugal
AB - The use of calendula for its lenitive properties’ dates to the XII century. This plant contains
several bioactive compounds, including terpenoids, terpenes, carotenoids, flavonoids and
polyunsaturated fatty acids. Calendula flower extract is used in soothing cosmetics, such as
after-sun, sensitive skin and eye contour products. The anti-inflammatory properties of this
ingredient were demonstrated in an animal model, but the mechanism of action is poorly
understood. Therefore, our work explored the effect of a calendula flower extract on NO
production, a pro-inflammatory radical produced by nitric oxide synthase (iNOS) and highly
released by innate immune cells in inflammatory-related pathologies. NO production was
evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) in macrophages, using
concentrations that did not compromise cells viability. This ingredient exhibited a
dose-dependent NO inhibition, reaching 50% at 147 µL/mL without cytotoxicity. Together with
previous literature, these results provide experimental evidence on the anti-inflammatory
properties of calendula flower extract, as well as its usefulness in cosmetics with soothing
properties and adjunctive skin care in the treatment of the diseases associated with
dysregulation of the NO signaling cascade. © 2021 by the authors. Licensee MDPI, Basel,
Switzerland.
KW - Anti-inflammatory
KW - Calendula
KW - INOS
KW - NO
KW - Skin care
KW - Soothing cosmetics
PB - MDPI AG
SN - 20799284 (ISSN)
LA - English
J2 - Cosmetics
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 11; Correspondence Address: I.F. Almeida;
Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy,
University of Porto, Porto, 4050-313, Portugal; email: ifalmeida@ff.up.pt
ER -

TY - JOUR
AU - Saeedeh, R.
AU - Mehrnaz, H.
AU - Mansour, G.
TI - Effect of seed priming with nanosilicon on morpho-physiological characterestics, quercetin
content and antioxidant capacity in calendula officinalis L. Under drought stress conditions
PY - 2020
T2 - Journal of Medicinal Plants
VL - 18
IS - 72
SP - 186
EP - 203
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082201158&partnerID=40&md5=12d9
570727d35c4d1d0ec7f855fd3fea
AD - Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak
University, Arak, Iran
AB - Background: Silicon is the second most structural element in the earth, which in plants, in
induces physiological processes and causes resistance to biotic and abiotic stresses as well.
Objective: The effect of seed priming with nanosilicon under different levels of drought stress on
morphological, physiological, total phenol and flavonoid contents, quercetin levels and
antioxidant capacity of the Calendula officinalis L. Method: This study was conducted as
factorial experiment in a randomized complete block design (RCBD) under four levels of drought
stress (25, 50, 75 and 100 %FC), and different concentrations (0, 100, 200 and 500 mg/L) of
silicon nanoparticles was considered as seed priming treatment. Results: The results showed
that drought stress levels and seed priming with nanosilicon at the various concentrations
caused significant changes (P < 0.05) on measured traits of plant. The highest antioxidant
activity of the obtained extract was observed in plants pretreated with nanosilicone at 200 mg/L
under 25 %FC. Drought stress at moderate level (50 %FC) along with seed priming with
nanosilicone at 100 mg /L had the highest effect on quercetin content. Furthermore, the highest
and the lowest content of total flavonoid was observed in plants pretreated with silicon
nanoparticles at 200 mg/L and control (without priming) under drought stress at 25 %FC,
respectively. Conclusion: Applicationof nanosilicone at lower concentrations (100-200 mg/L) and
drought stress at 50 %FC may improve plant physiological and metabolite indices in marigold. ©
2020 Institute of Medicinal Plants. All rights reserved.
KW - Calendula officinalis
KW - Growth
KW - Nano silicon
KW - Priming
KW - Quarcetin
KW - Water deficit stress
KW - flavonoid
KW - quercetin
KW - silica nanoparticle
KW - antioxidant activity
KW - Article
KW - Calendula officinalis
KW - concentration (parameter)
KW - controlled study
KW - drought stress
KW - metabolite
KW - nonhuman
KW - physiological process
KW - plant seed
PB - Institute of Medicinal Plants
SN - 2717204X (ISSN)
LA - Persian
J2 - J. Med. Plants
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 8; Correspondence Address: H. Mehrnaz;
Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University,
Arak, 38156-8-8349, Iran; email: m-hatami@araku.ac.ir
ER -

TY - JOUR
AU - Oniga, I.
AU - Toiu, A.
AU - Hanganu, D.
AU - Vlase, L.
AU - Duda, M.
AU - Benedec, D.
TI - Influence of fertilizer treatment on the chemical composition of some calendula officinalis
varieties cultivated in Romania
PY - 2018
T2 - Farmacia
VL - 66
IS - 6
SP - 995
EP - 998
DO - 10.31925/FARMACIA.2018.6.11
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059091530&doi=10.31925%2fFARMA
CIA.2018.6.11&partnerID=40&md5=8063cabc9c60757b8f1c016b4216bfb3
AD - “Iuliu Haţieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, 12 Ion
Creangă Street, Cluj-Napoca, 400010, Romania
AD - University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of
Agriculture, Department of Phytotechnology, 3-5 Mănăștur Alley, Cluj-Napoca, 400372,
Romania
AB - In order to select a good genetic material for cultivation, the influence of fertilizer on the
biosynthesis of active principles from the inflorescences of six Calendula officinalis L. (marigold)
varieties was investigated. The marigold flowers were cultivated and harvested from Romania,
Cluj County. The qualitative and quantitative analyses were performed by spectrophotometric
methods and HPLC. The concentrations of flavonoids (0.43-0.66%, expressed in rutin),
phenylpropane derivatives (0.21-1.25%, expressed in caffeic acid) and carotenoids
(0.40-0.75%, expressed in beta-carotene) were determined. Although the ‘Agrosel 696’ and
‘Starsem 922’ varieties were the richest in flavonoids and phenylpropane derivatives, other
varieties, as ‘Cluj 2’ and ‘Novi Sad’ may be preferred for cultivation as natural sources of
carotenoids. The use of fertilizer stimulated, in small degree, the biosynthesis of flavonoids, but
it decreased the quantities of carotenoids and phenylpropane derivatives. All samples were in
accordance with the requirements of the European Pharmacopoeia (min 0.4% flavonoids). ©
2018, Romanian Society for Pharmaceutical Sciences. All rights reserved.
KW - Calendula officinalis
KW - Carotenoids
KW - Fertilizer
KW - Flavonoids
PB - Romanian Society for Pharmaceutical Sciences
SN - 00148237 (ISSN)
LA - English
J2 - Farmacia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2; Correspondence Address: A. Toiu; “Iuliu
Haţieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Cluj-Napoca, 12 Ion
Creangă Street, 400010, Romania; email: ancamaria_toiu@yahoo.com; CODEN: FRMBA
ER -

TY - JOUR
AU - Santos, A.P.
AU - Gonçalves, M.M.
AU - Justus, B.
AU - da Silva Fardin, D.P.
AU - Toledo, A.C.O.
AU - Budel, J.M.
AU - Paula, J.P.
TI - Calendula officinalis L. flower extract-mediated green synthesis of silver nanoparticles
under LED light
PY - 2022
T2 - Brazilian Journal of Pharmaceutical Sciences
VL - 58
C7 - e19519
DO - 10.1590/s2175-97902022e19519
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133830301&doi=10.1590%2fs2175-97
902022e19519&partnerID=40&md5=59e00c8a93a62677b63caee6283ea72a
AD - Postgraduate Program in Pharmaceutical Sciences, State University of Ponta Grossa,
Paraná, Ponta Grossa, Brazil
AB - Silver nanoparticles (AgNPs) are among the most known nanomaterials being used for
several purposes, including medical applications. In this study, Calendula officinalis L. flower
extract and silver nitrate were used for green synthesis of silver nanoparticles under red, green
and blue light-emitting diodes. AgNPs were characterized by Ultraviolet-Visible
Spectrophotometry, Field Emission Scanning Electron Microscopy, Dynamic Light Scattering,
Electrophoretic Mobility, Fourier Transform Infrared Spectroscopy and X-ray Diffraction. Isotropic
and anisotropic silver nanoparticles were obtained, presenting hydrodinamic diameters ranging
90 – 180 nm, polydispersity (PdI > 0.2) and moderate stability (zeta potential values around – 20
mV). © 2022, Faculdade de Ciencias Farmaceuticas (Biblioteca). All rights reserved.
KW - Metal nanoparticle
KW - Nanotechnology
KW - Surface plasmon resonance
KW - Calendula officinalis extract
KW - flower extract
KW - silver nanoparticle
KW - silver nitrate
KW - unclassified drug
KW - Article
KW - dispersity
KW - drug synthesis
KW - electrophoretic mobility
KW - field emission scanning electron microscopy
KW - Fourier transform infrared spectroscopy
KW - nanotechnology
KW - nonhuman
KW - photon correlation spectroscopy
KW - physical chemistry
KW - ultraviolet visible spectrophotometry
KW - X ray diffraction
KW - zeta potential
PB - Faculdade de Ciencias Farmaceuticas (Biblioteca)
SN - 19848250 (ISSN)
LA - English
J2 - Bras. J. Pharm. Sci.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 3; Correspondence Address: M.M. Gonçalves;
Universidade Federal do Paraná, Curitiba, Av. Prefeito Lothário Meissner, 632. Jardim Botânico,
Paraná, 80210-170, Brazil; email: melissamg1106@gmail.com
ER -

TY - JOUR
AU - Millán, D.
AU - Jiménez, R.A.
AU - Nieto, L.E.
AU - Linero, I.
AU - Laverde, M.
AU - Fontanilla, M.R.
TI - Preclinical evaluation of collagen type I scaffolds, including gelatin-collagen microparticles
and loaded with a hydroglycolic Calendula officinalis extract in a lagomorph model of
full-thickness skin wound
PY - 2016
T2 - Drug Delivery and Translational Research
VL - 6
IS - 1
SP - 57
EP - 66
DO - 10.1007/s13346-015-0265-8
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954518488&doi=10.1007%2fs13346-0
15-0265-8&partnerID=40&md5=6ec3659774c7676eb25ee01180fd5f14
AD - Tissue Engineering Group, Pharmacy Department, Universidad Nacional de Colombia, Av.
Carrera 30 # 45-10, Bogotá, Colombia
AD - School of Medicine, Universidad el Bosque, Bogotá, Colombia
AD - School of Medicine, Pontificia Universidad Javeriana, Bogotá, Colombia
AD - Hospital Militar Central, Bogotá, Colombia
AD - School of Dentistry, Universidad Nacional de Colombia, Bogotá, Colombia
AD - School of Dentistry, Universidad Santo Tomas, Bogotá, Colombia
AB - Previously, we have developed collagen type I scaffolds including microparticles of
gelatin-collagen type I (SGC) that are able to control the release of a hydroglycolic extract of the
Calendula officinalis flower. The main goal of the present work was to carry out the preclinical
evaluation of SGC alone or loaded with the C. officinalis extract (SGC-E) in a lagomorph model
of full-thickness skin wound. A total of 39 rabbits were distributed in three groups, of 13 animals
each. The first group was used to compare wound healing by secondary intention (control) with
wound healing observed when wounds were grafted with SGC alone. Comparison of control
wounds with wounds grafted with SGC-E was performed in the second group, and comparison
of wounds grafted with SGC with wounds grafted with SGC-E was performed in the third group.
Clinical follow-ups were carried in all animals after surgery, and histological and
histomorphometric analyses were performed on tissues taken from the healed area and healthy
surrounding tissue. Histological and histomorphometric results indicate that grafting of SGC
alone favors wound healing and brings a better clinical outcome than grafting SGC-E. In vitro
collagenase digestion data suggested that the association of the C. officinalis extract to SGC
increased the SGC-E cross-linking, making it difficult to degrade and affecting its
biocompatibility. © 2015, Controlled Release Society.
KW - Calendula officinalis L. flowers extract
KW - Collagen type I
KW - Full-thickness wounds
KW - Gelatin-collagen microparticles
KW - Scaffolds
KW - Animals
KW - Calendula
KW - Collagen Type I
KW - Drug Evaluation, Preclinical
KW - Flowers
KW - Gelatin
KW - Male
KW - Models, Animal
KW - Plant Extracts
KW - Rabbits
KW - Skin
KW - Tissue Scaffolds
KW - Wound Healing
KW - Calendula extract
KW - Calendula officinalis extract
KW - collagen type 1
KW - collagenase
KW - gelatin
KW - glycolic acid
KW - unclassified drug
KW - collagen type 1
KW - gelatin
KW - plant extract
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - biocompatibility
KW - Calendula officinalis
KW - controlled study
KW - drug delivery system
KW - flower
KW - follow up
KW - histology
KW - in vitro study
KW - male
KW - membrane microparticle
KW - morphometrics
KW - New Zealand rabbit
KW - nonhuman
KW - priority journal
KW - protein cross linking
KW - skin biopsy
KW - skin injury
KW - solvent extraction
KW - tissue scaffold
KW - wound healing
KW - animal
KW - Calendula
KW - drug effects
KW - injuries
KW - preclinical study
KW - rabbit
KW - skin
KW - tissue scaffold
PB - Springer Verlag
SN - 2190393X (ISSN)
C2 - 26597789
LA - English
J2 - Drug Deliv. Transl. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 9; Correspondence Address: M.R. Fontanilla;
Tissue Engineering Group, Pharmacy Department, Universidad Nacional de Colombia, Bogotá,
Av. Carrera 30 # 45-10, Colombia; email: mrfontanillad@unal.edu.co
ER -

TY - JOUR
AU - Mahyari, S.
AU - Mahyari, B.
AU - Emami, S.A.
AU - Malaekeh-Nikouei, B.
AU - Jahanbakhsh, S.P.
AU - Sahebkar, A.
AU - Mohammadpour, A.H.
TI - Evaluation of the efficacy of a polyherbal mouthwash containing Zingiber officinale,
Rosmarinus officinalis and Calendula officinalis extracts in patients with gingivitis: A randomized
double-blind placebo-controlled trial
PY - 2016
T2 - Complementary Therapies in Clinical Practice
VL - 22
SP - 93
EP - 98
DO - 10.1016/j.ctcp.2015.12.001
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958068546&doi=10.1016%2fj.ctcp.201
5.12.001&partnerID=40&md5=f228126c7ca252de48b76dec19af8c89
AD - Pharmaceutical Research Center, Department of Clinical Pharmacy, School of Pharmacy,
Mashhad University of Medical Sciences, Mashhad, Iran
AD - Mahyari Dentistry Clinic, Iran Square, Neyshabour, Iran
AD - Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical
Sciences, Mashhad, Iran
AD - Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical
Sciences, Mashhad, Iran
AD - Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical
Sciences, Mashhad, Iran
AD - Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
AD - Metabolic Research Centre, Royal Perth Hospital, School of Medicine and Pharmacology,
University of Western Australia, Perth, Australia
AB - Background: Gingivitis is a highly prevalent periodontal disease resulting from microbial
infection and subsequent inflammation. The efficacy of herbal preparations in subjects with
gingivitis has been reported in some previous studies. Objective: To investigate the efficacy of a
polyherbal mouthwash containing hydroalcoholic extracts of Zingiber officinale, Rosmarinus
officinalis and Calendula officinalis (5% v/w) compared with chlorhexidine and placebo
mouthwashes in subjects with gingivitis. Methods: Sixty patients participated in this randomized
double-blind placebo-controlled trial and were randomly assigned to the polyherbal mouthwash
(n = 20), chlorhexidine mouthwash (n = 20) or placebo mouthwash (n = 20). Participants were
instructed to use the mouthwash twice a day (after breakfast and dinner) for 30 s for a period of
two weeks. Gingival and plaque indices were assessed using MGI, GBI and MQH scales at
baseline, day 7 and day 14 of the trial. Results: There were significant improvements in all
assessed efficacy measures i.e. MGI, GBI and MQH scores from baseline to the end of trial in
both polyherbal and chlorhexidine mouthwash groups; however, the scores remained
statistically unchanged in the placebo group. MGI, BGI and MQH scores in the treatment groups
were significantly lower compared with those of the control group at both day 7 and day 14 of
the trial. However, there was no significant difference between the polyherbal and chlorhexidine
groups, neither at day 7 nor day 14 of the trial. Polyherbal mouthwash was safe and there was
neither report of adverse reactions, nor any drop-out during the course of study. Conclusion:
Polyherbal mouthwash containing hydroalcoholic extracts of Z. officinale, R. officinalis and C.
officinalis (5%) was effective in the treatment of gingivitis and its efficacy was comparable to that
of chlorhexidine mouthwash. © 2015 Elsevier Ltd.
KW - Ginger
KW - Gingivitis
KW - Marigold
KW - Randomized controlled trial
KW - Rosemary
KW - Adult
KW - Calendula
KW - Chlorhexidine
KW - Dental Plaque Index
KW - Female
KW - Ginger
KW - Gingivitis
KW - Humans
KW - Male
KW - Middle Aged
KW - Mouthwashes
KW - Plant Extracts
KW - Rosmarinus
KW - Young Adult
KW - chlorhexidine
KW - mouthwash
KW - plant extract
KW - adult
KW - Calendula
KW - controlled study
KW - female
KW - ginger
KW - gingivitis
KW - human
KW - male
KW - middle aged
KW - plaque index
KW - randomized controlled trial
KW - Rosmarinus
KW - young adult
PB - Churchill Livingstone
SN - 17443881 (ISSN)
C2 - 26850813
LA - English
J2 - Complement. Ther. Clin. Pract.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 42; Correspondence Address: A.H.
Mohammadpour; Pharmaceutical Research Center, Department of Clinical Pharmacy, School of
Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; email:
MohamadpoorAH@mums.ac.ir
ER -

TY - JOUR
AU - Dinda, M.
AU - Mazumdar, S.
AU - Das, S.
AU - Ganguly, D.
AU - Dasgupta, U.B.
AU - Dutta, A.
AU - Jana, K.
AU - Karmakar, P.
TI - The Water Fraction of Calendula officinalis Hydroethanol Extract Stimulates In Vitro and In
Vivo Proliferation of Dermal Fibroblasts in Wound Healing
PY - 2016
T2 - Phytotherapy Research
SP - 1696
EP - 1707
DO - 10.1002/ptr.5678
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989876716&doi=10.1002%2fptr.5678&
partnerID=40&md5=3f859824ae2bd2b9a48d9799fe0c2418
AD - Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S.C.
Mullick Road, Kolkata, 700 032, West Bengal, India
AD - Department of Chemistry (Inorganic Section), Jadavpur University, 188, Raja S.C. Mullick
Road, Kolkata, 700 032, West Bengal, India
AD - Division of Molecular Medicine, Bose Institute, P 1/12, CIT Scheme VIIM, Kolkata, 700
054, India
AB - The active fraction and/or compounds of Calendula officinalis responsible for wound
healing are not known yet. In this work we studied the molecular target of C. officinalis
hydroethanol extract (CEE) and its active fraction (water fraction of hydroethanol extract,
WCEE) on primary human dermal fibroblasts (HDF). In vivo, CEE or WCEE were topically
applied on excisional wounds of BALB/c mice and the rate of wound contraction and
immunohistological studies were carried out. We found that CEE and only its WCEE significantly
stimulated the proliferation as well as the migration of HDF cells. Also they up-regulate the
expression of connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in
vitro. In vivo, CEE or WCEE treated mice groups showed faster wound healing and increased
expression of CTGF and α-SMA compared to placebo control group. The increased expression
of both the proteins during granulation phase of wound repair demonstrated the potential role of
C. officinalis in wound healing. In addition, HPLC-ESI MS analysis of the active water fraction
revealed the presence of two major compounds, rutin and quercetin-3-O-glucoside. Thus, our
results showed that C. officinalis potentiated wound healing by stimulating the expression of
CTGF and α-SMA and further we identified active compounds. Copyright © 2016 John Wiley &
Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.
KW - Calendula officinalis hydroethanol extract (CEE)
KW - CTGF
KW - water fraction of Calendula officinalis hydroethanol extract (WCEE)
KW - wound healing
KW - α-SMA
KW - alcohol
KW - alpha smooth muscle actin
KW - Calendula officinalis extract
KW - connective tissue growth factor
KW - flavonoid
KW - isoquercitrin
KW - phenol derivative
KW - placebo
KW - plant extract
KW - rutoside
KW - unclassified drug
KW - water
KW - wound healing promoting agent
KW - adult
KW - animal cell
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - cell migration
KW - cell proliferation
KW - cell stimulation
KW - controlled study
KW - drug targeting
KW - excision
KW - flower
KW - human
KW - human cell
KW - immunohistology
KW - in vitro study
KW - in vivo study
KW - male
KW - nonhuman
KW - skin fibroblast
KW - upregulation
KW - wound
KW - wound contraction
KW - wound healing
PB - John Wiley and Sons Ltd
SN - 0951418X (ISSN)
C2 - 27426257
LA - English
J2 - Phytother. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 36; Correspondence Address: P. Karmakar;
Department of Life Science and Biotechnology, Jadavpur University, Kolkata, 188, Raja S.C.
Mullick Road, 700 032, India; email: pkarmakar_28@yahoo.co.in; CODEN: PHYRE
ER -

TY - JOUR
AU - Randive, D.S.
AU - Bhinge, S.D.
AU - Bhutkar, M.A.
AU - Joshi, S.R.
AU - Patil, P.D.
AU - Shejawal, K.P.
AU - Thorat, M.S.
AU - Mulla, A.S.
TI - Formulation and evaluation of Herbal cough remedy from extract of Calendula officinalis L.
PY - 2020
T2 - Indian Drugs
VL - 57
IS - 4
SP - 52
EP - 58
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087855308&partnerID=40&md5=4f634
390c598baea3a388c3c58d402ec
AD - Department of Pharmaceutics
AD - Department of Pharmaceutical chemistry
AD - Department of Quality Assurance, Rajarambapu College of Pharmacy, Kasegaon, Walwa,
Sangli Maharashtra, 415404, India
AB - In the recent years, the interests on the herbal medicines and their utilization for the
treatment for various diseases have been increasing rapidly, even in those areas where modern
medicine is available. Medicinal plants are the richest source of bioactive compounds used in
the traditional and modern medicine. Nowadays, demand for the plant based medicines, health
products, pharmaceuticals, food supplements has been increasing due to their effectiveness.
The objective of the present study was to formulate and evaluate herbal cough syrup from the
petal extracts of Calendula officinalis L. The syrup formulation was designed by using petal
extract, honey and required amount of distilled water. The prepared herbal cough syrup was
evaluated for different parameters such as pH, color, odor, taste, thermal stability, turbidity,
accelerated stability studies, HPTLC and antimicrobial activity against different microbial strains
namely Aspergillus Niger, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis,
Staphylococcus aureus and E. coli. HPTLC analysis of the prepared formulations was
compared with standard marker compound namely Quercetin. The Rf of Quercetin perfectly
matched with the spot observed in Formulation A and B. From the results of the study, it can be
concluded that the prepared syrup formulation comprising of aqueous extract of C. officinalis
exhibited good antimicrobial activity against the selected microbial strains, and it exhibited
soothing effect with sweet taste because of honey. © 2020 Indian Drug Manufacturers'
Association. All rights reserved.
KW - Antimicrobial activity
KW - Calendula officinalis L
KW - Herbal cough syrup
KW - antitussive agent
KW - Calendula officinalis extract
KW - plant extract
KW - quercetin
KW - unclassified drug
KW - antitussive activity
KW - Article
KW - Aspergillus niger
KW - Bacillus subtilis
KW - bacterial strain
KW - Calendula officinalis
KW - color
KW - controlled study
KW - drug formulation
KW - Escherichia coli
KW - high performance thin layer chromatography
KW - Klebsiella pneumoniae
KW - nonhuman
KW - pH
KW - physical appearance
KW - physical chemistry
KW - Pseudomonas
KW - Pseudomonas aeruginosa
KW - relative density
KW - Staphylococcus aureus
KW - sweetness
KW - syrup
KW - thermostability
KW - turbidity
KW - zone of inhibition
PB - Indian Drug Manufacturers' Association
SN - 0019462X (ISSN)
LA - English
J2 - Indian Drugs
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: D.S. Randive;
Department of Pharmaceutics; email: randivedheeraj@gmail.com; CODEN: INDRB
ER -

TY - JOUR
AU - Buzzi, M.
AU - De Freitas, F.
AU - Winter, M.
TI - A Prospective, Descriptive Study to Assess the Clinical Benefits of Using Calendula
officinalis Hydroglycolic Extract for the Topical Treatment of Diabetic Foot Ulcers
PY - 2016
T2 - Ostomy Wound Management
VL - 62
IS - 3
SP - 8
EP - 24
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961777776&partnerID=40&md5=585ff
7cbb02296e36b72667c54c9b4fd
AD - Proamplus Clinical Research Advisory LTD, Pinhais, Paraná, Brazil
AD - Phytoplenus Bioativos S.A., Pinhais, Paraná, Brazil
AD - Hospital da Santa Casa de Misericórdia de Curitiba, Department of Dermatology, Curitiba,
Paraná, Brazil
AB - Diabetic foot ulcers (DFUs) have a significant impact on patient quality of life. A
prospective, descriptive pilot study was conducted between May 2012 and December 2013
through the dermatology outpatient unit in a Brazilian hospital to evaluate the clinical benefits of
using Calendula officinalis hydroglycolic extract in the treatment of DFUs. Patients diagnosed
with a stable neuropathic ulcer of >3 months' duration; ranging in size from 0.5-40 cm2; without
osteomyelitis, gangrene, bone exposure, cancer, or deep tissue infection; ages 18-90 years;
with adequate glycemic control and no history of an allergy to C. officinalis were enrolled.
Patients provided demographic and diabetes-related information and were evaluated biweekly
for 30 weeks or until healing (ie, full epithelialization with no wound drainage). DFUs were
measured and clinically examined for microbiological flora and presence of odor, tissue type
(eg, granulation, fibrin sloth, necrosis), exudate, and retraction rate using planimetry images.
Patients' blood tests and neuropathic pain assessment (the latter by clinician-directed
questionnaire) were performed at baseline and the end of treatment; pain also was assessed
during dressing changes using a 10-point rating scale. Patients' ulcers were treated twice daily
with C. officinalis hydroglycolic extract spray solution and covered with saline-moistened, sterile,
nonadherent gauze and bandages followed by foot offloading with adequate protective footwear.
Patients received their first treatment in the clinic then performed care at home. From a potential
population of 109 patients, 25 did not meet the inclusion criteria. Of the remaining 84
participants enrolled, 43 withdrew before study completion; cited reasons included lost to
follow-up (16), medical judgment (2), failure to attend >3 scheduled visits (17), protocol violation
(5), and death (3). Forty-one (41)-17 women, average age 62 years (range 44-82 years),
average glycemic level 153 mg/dL (range 82-395 mg/dL), most (34) with Wagner type 1
ulcers-completed the study. The proportions of patients who achieved complete wound closure
after 11, 20, and 30 weeks of treatment was 54%, 68%, and 78%, respectively; mean healing
time was 15.5 ± 6.7 weeks. When individual weekly healing rates (the percentage reduction in
wound area per week) were corrected for variability in initial DFU area, the values were nearly
6-fold higher for complete wound closure (7.8% ± 3.6%) than for incomplete wound closure
(1.4% ± 0.7%) (Student t-test; P = 0.001). After 30 weeks of treatment, the number of colonized
wounds decreased from 29 at baseline to 5, and the number of odorous wounds decreased
from 19 to 1. Ulcer bed planimetry data showed a significant reduction in the amount of exudate,
fibrin slough, and necrotic tissue after the treatment with C. officinalis hydroglycolic extract (Χ2
test; P = 0.001). No adverse events were observed during treatment. The study findings suggest
C. officinalis extract is safe and has a beneficial effect on DFU healing. Randomized, controlled
studies using C. officinalis hydroglycolic extract are warranted to confirm its safety and establish
its clinical efficacy and effectiveness for the topical treatment of DFUs.
KW - Calendula
KW - Clinical study
KW - diabetes complications
KW - diabetic foot
KW - wound healing
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Brazil
KW - Calendula
KW - Diabetic Foot
KW - Female
KW - Humans
KW - Male
KW - Middle Aged
KW - Phytotherapy
KW - Pilot Projects
KW - Plant Extracts
KW - Prospective Studies
KW - plant extract
KW - adult
KW - aged
KW - Brazil
KW - Calendula
KW - clinical trial
KW - diabetic foot
KW - female
KW - human
KW - male
KW - middle aged
KW - pathology
KW - phytotherapy
KW - pilot study
KW - prospective study
KW - very elderly
PB - HMP Communications
SN - 08895899 (ISSN)
C2 - 26978856
LA - English
J2 - Ostomy Wound Manage.
M3 - Review
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 14; Correspondence Address: M. Buzzi;
Proamplus Clinical Research Advisory LTD, Pinhais, Paraná, Brazil; email:
marcelo.buzzi@gmail.com
ER -

TY - JOUR
AU - Dall'Acqua, S.
AU - Catanzaro, D.
AU - Cocetta, V.
AU - Igl, N.
AU - Ragazzi, E.
AU - Giron, M.C.
AU - Cecconello, L.
AU - Montopoli, M.
TI - Protective effects of ψ taraxasterol 3-O-myristate and arnidiol 3-O-myristate isolated from
Calendula officinalis on epithelial intestinal barrier
PY - 2016
T2 - Fitoterapia
VL - 109
SP - 230
EP - 235
DO - 10.1016/j.fitote.2016.01.007
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955576829&doi=10.1016%2fj.fitote.20
16.01.007&partnerID=40&md5=0ab67933a6dc7788436e522322e4ebe6
AD - Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via
Marzolo 5, Padova, Italy
AD - NATECO2 GmbH and Co. KG, Auenstrasse 18-20, Wolnzach, 85283, Germany
AB - The triterpene esters Ψ taraxasterol-3-O-myristate (1) and arnidiol-3-O-myristate (2) were
tested for their ability to protect epithelial intestinal barrier in an in vitro model. Their effects on
ROS production and on trans-epithelial resistance were investigated on CaCo-2 cell monolayers
both in basal and stress-induced conditions. Both compounds were able to modulate the stress
damage induced by H2O2 and INFγ + TNFα, showing a potential use as model compounds for
the study of new therapeutic agents for intestinal inflammations. © 2015 Elsevier B.V. All rights
reserved.
KW - CaCo-2 cell monolayers
KW - Calendula officinalis
KW - Intestinal inflammation
KW - ROS
KW - TEER
KW - Triterpene
KW - Caco-2 Cells
KW - Calendula
KW - Epithelial Cells
KW - Flowers
KW - Humans
KW - Hydrogen Peroxide
KW - Molecular Structure
KW - Myristates
KW - Reactive Oxygen Species
KW - Triterpenes
KW - arnidiol 3 o myristate
KW - gamma interferon
KW - hydrogen peroxide
KW - reactive oxygen metabolite
KW - taraxasterol 3 o myristate psi
KW - triterpene derivative
KW - tumor necrosis factor alpha
KW - unclassified drug
KW - arnidiol-3-O-myristate
KW - myristic acid derivative
KW - reactive oxygen metabolite
KW - taraxasterol-3-O-myristate
KW - triterpene
KW - Article
KW - basal cell
KW - CACO 2 cell line
KW - Calendula officinalis
KW - cell protection
KW - cell viability
KW - controlled study
KW - drug effect
KW - epithelial intestinal barrier
KW - human
KW - human cell
KW - in vitro study
KW - intestine cell
KW - intestine epithelium
KW - oxidative stress
KW - priority journal
KW - transepithelial resistance
KW - Calendula
KW - chemical structure
KW - chemistry
KW - drug effects
KW - epithelium cell
KW - flower
KW - isolation and purification
KW - metabolism
PB - Elsevier B.V.
SN - 0367326X (ISSN)
C2 - 26791917
LA - English
J2 - Fitoterapia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 9; Correspondence Address: S. Dall'Acqua;
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova,
via Marzolo 5, Italy; email: stefano.dallacqua@unipd.it; CODEN: FTRPA
ER -

TY - JOUR
AU - Moraes, M.L.L.
AU - da Silva, H.D.T.
AU - Blanes, L.
AU - Dobb, P.
AU - Tavares, M.F.M.
TI - Optimization of chemometric approaches for the extraction of isorhamnetin-3-O-rutinoside
from Calendula officinalis L.
PY - 2016
T2 - Journal of Pharmaceutical and Biomedical Analysis
VL - 125
SP - 408
EP - 414
DO - 10.1016/j.jpba.2016.04.017
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84964490186&doi=10.1016%2fj.jpba.201
6.04.017&partnerID=40&md5=a2f7b6bc882bfddbb1a53af77471a712
AD - Laboratory of Applied Chemical Analysis, Department of Exact and Earth Sciences,
Federal University of São Paulo, Diadema, SP, Brazil
AD - Centre for Forensic Science, University of Technology, Sydney, Broadway, NSW, Australia
AD - Laboratory of Chromatography and Capillary Electrophoresis, Department of Fundamental
Chemistry, University of São Paulo, São Paulo, SP, Brazil
AB - The application of Design of Experiments (DoE) to the determination of optimum
conditions for an extraction process relies on the correct selection of mathematical models. The
linear model is the one typically used; however, in some cases it does not always have superior
performance, ignoring the real nature of the data and its appropriate descriptive model. In order
to evaluate the extraction efficiency of isorhamnetin-3-O-rutinoside from flowers of Calendula
officinalis L. a multivariate factorial analysis was used. Simulations were conducted using linear,
quadratic, full cubic and special cubic models. A Simplex-Centroid design was chosen as it
delivered greater precision with only minor errors versus other models tested. Analyses were
performed by capillary zone electrophoresis using sodium tetraborate buffer (40 mmol L-1, pH
9.4) containing 10% methanol. The detection was linear over a range of 8.0-50.0 mg L-1 (r2 =
0.996), and the limits of detection (LOD) and quantification (LOQ) for
isorhamnetin-3-O-rutinoside were 3.44 mg L-1 and 11.47 mg L-1, respectively. The full cubic
model showed the best extraction results, with an error of 3.40% compared to analysis of
variance, and a determination coefficient of 0.974. The difference between the responses at the
reference point, calculated by the model, and the experimental response, varies around 2.72%
for full cubic model. Comparison of the four models showed the full cubic model was the most
appropriate one, allowing greater efficiency in the extraction of isorhamnetin-3-O-rutinoside.
Selection of the model made it possible to obtain a 60% increase in sensitivity compared to the
linear model. © 2016.
KW - Calendula officinalis L.
KW - Capillary electrophoresis
KW - Chemometrics
KW - Factorial design
KW - Isorhamnetin-3-O-rutinoside
KW - Marigold
KW - Calendula
KW - Disaccharides
KW - Flavonoids
KW - Limit of Detection
KW - Reproducibility of Results
KW - Calendula extract
KW - Calendula officinalis extract
KW - isorhamnetin 3 o rutinoside
KW - unclassified drug
KW - disaccharide
KW - flavonoid
KW - isorhamnetin 3-O-rutinoside
KW - Article
KW - Calendula officinalis
KW - capillary zone electrophoresis
KW - chemometrics
KW - flower
KW - limit of detection
KW - limit of quantitation
KW - mathematical model
KW - nonhuman
KW - priority journal
KW - process optimization
KW - simulation
KW - Calendula
KW - chemistry
KW - isolation and purification
KW - reproducibility
KW - validation study
PB - Elsevier B.V.
SN - 07317085 (ISSN)
C2 - 27131151
LA - English
J2 - J. Pharm. Biomed. Anal.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 10; Correspondence Address: M.L.L. Moraes;
Laboratório de Análises Químicas Aplicadas, Departamento de Ciências Exatas e da Terra,
Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Rua Prof. Artur Riedel 275,
09972-270, Brazil; email: mllmoraes@unifesp.br; CODEN: JPBAD
ER -

TY - JOUR
AU - Saffari, E.
AU - Mohammad-Alizadeh-Charandabi, S.
AU - Adibpour, M.
AU - Mirghafourvand, M.
AU - Javadzadeh, Y.
TI - Comparing the effects of Calendula officinalis and clotrimazole on vaginal Candidiasis: A
randomized controlled trial
PY - 2017
T2 - Women and Health
VL - 57
IS - 10
SP - 1145
EP - 1160
DO - 10.1080/03630242.2016.1263272
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006856456&doi=10.1080%2f03630242
.2016.1263272&partnerID=40&md5=f2c27c74f98fe6ec009db78b873b19dd
AD - Students’ Research Committee, Department of Midwifery, Tabriz University of Medical
Sciences, Tabriz, Iran
AD - Social Determinants of Health Research Centre, Department of Midwifery, Tabriz
University of Medical Sciences, Tabriz, Iran
AD - Department of Parasitology, Tabriz University of Medical Sciences, Tabriz, Iran
AD - Department of Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran
AD - Department of Pharmaceutics, Tabriz University of Medical Sciences, Tabriz, Iran
AB - This triple-blind trial examined the effects of Calendula officinalis vaginal cream on the
treatment of vaginal Candidiasis (primary outcome) and sexual function (secondary outcome).
Married women aged 18–45 years with vaginal Candidiasis (n = 150) were recruited from April
to October 2014 and randomized into Calendula and clotrimazole groups, using 5-g vaginal
cream every night for seven nights. Clinical and laboratory assessments were conducted at
10–15 and 30–35 days after intervention and the female sexual function index was assessed at
30–35 days. Six women were lost to follow-up. The frequency of testing negative for Candidiasis
in the Calendula group was significantly lower at the first (49% vs. 74%; odds ratio (OR) 0.32;
95% confidence interval (CI) 0.16–0.67) but higher at the second (77% vs. 34%; OR 3.1; 95%
CI 1.5–6.2) follow-up compared to the clotrimazole group. The frequency of most signs and
symptoms were almost equal in the two groups at the first follow-up, but were significantly lower
in the Calendula group at the second follow-up. Sexual function had almost equal significant
improvement in both groups. Calendula vaginal cream appears to have been effective in the
treatment of vaginal Candidiasis and to have a delayed but greater long-term effect compared to
clotrimazole. © 2016 Taylor & Francis.
KW - Calendula officinalis
KW - clotrimazole
KW - sexual function
KW - vaginal Candidiasis
KW - Administration, Intravaginal
KW - Adolescent
KW - Adult
KW - Calendula
KW - Candidiasis, Vulvovaginal
KW - Clotrimazole
KW - Female
KW - Humans
KW - Middle Aged
KW - Plant Extracts
KW - Treatment Outcome
KW - Vaginal Creams, Foams, and Jellies
KW - Young Adult
KW - agents used intravaginally
KW - clotrimazole
KW - plant extract
KW - adolescent
KW - adult
KW - Calendula
KW - controlled study
KW - female
KW - human
KW - intravaginal drug administration
KW - middle aged
KW - randomized controlled trial
KW - treatment outcome
KW - vagina candidiasis
KW - young adult
PB - Routledge
SN - 03630242 (ISSN)
C2 - 27880086
LA - English
J2 - Women Health
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 15; Correspondence Address: S.
Mohammad-Alizadeh-Charandabi; Social Determinants of Health Research Centre, Department
of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, PO
Box 51745-347, 5138947977, Iran; email: alizades@tbzmed.ac.ir; CODEN: WOHED
ER -

TY - JOUR
AU - Hernández-Saavedra, D.
AU - Pérez-Ramírez, I.F.
AU - Ramos-Gómez, M.
AU - Mendoza-Díaz, S.
AU - Loarca-Piña, G.
AU - Reynoso-Camacho, R.
TI - Phytochemical characterization and effect of Calendula officinalis, Hypericum perforatum,
and Salvia officinalis infusions on obesity-associated cardiovascular risk
PY - 2016
T2 - Medicinal Chemistry Research
VL - 25
IS - 1
SP - 163
EP - 172
DO - 10.1007/s00044-015-1454-1
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954219070&doi=10.1007%2fs00044-0
15-1454-1&partnerID=40&md5=ae143a17b5df87873ddb8613283aa232
AD - Research and Graduate Studies in Food Science, School of Chemistry, Universidad
Autonoma de Queretaro, Queretaro, 76010, Mexico
AB - Medicinal herb infusions can be used for the treatment of obesity-related metabolic
alterations. The aim of this study was to characterize the phytochemical profile and to evaluate
the effect of Hypericum perforatum, Salvia officinalis, and Calendula officinalis on the
cardiovascular risk developed in diet-induced obese rats. All infusions decreased body weight
and abdominal fat mass and reduced serum triglycerides (TG), total cholesterol, low-density
lipoproteins, and C-reactive protein levels. The anti-obesogenic and hypolipidemic effect of C.
officinalis and H. perforatum were associated with the inhibition of triglycerides digestion and
absorption. Conversely, the hypolipidemic effect of S. officinalis was not associated with this
mechanism. Moreover, seven phenolic acids and nine flavonoids were quantified by
HPLC-UV/VIS, hesperidin and epigallocatechin gallate were the majoritarian compounds of H.
perforatum and C. officinalis, whereas rosmarinic and ellagic acids were the main compounds in
S. officinalis. Additionally, we used HPLC-DAD-MSD to identify 45 phytochemicals, such as
anthraquinone and phloroglucinol derivatives, phytosterols, saponins, and alkaloids. Total
flavonoids, phytosterols, and alkaloids content were highly correlated with TG levels, AUC
values from the oil tolerance test, and pancreatic lipase inhibition. These results suggest that
these phytochemical-rich infusions may be used as an alternative for obesity-related
cardiovascular risk treatment. © 2015 Springer Science+Business Media New York.
KW - Cardiovascular risk
KW - Medicinal herbs
KW - Obesity
KW - Oil tolerance test
KW - alkaloid derivative
KW - anthraquinone
KW - C reactive protein
KW - epigallocatechin gallate
KW - flavonoid
KW - hesperidin
KW - high density lipoprotein cholesterol
KW - low density lipoprotein cholesterol
KW - phenol derivative
KW - phloroglucinol derivative
KW - phytosterol
KW - saponin derivative
KW - triacylglycerol lipase
KW - animal experiment
KW - animal model
KW - area under the curve
KW - Article
KW - Calendula officinalis
KW - cardiovascular disease
KW - cardiovascular risk
KW - cholesterol blood level
KW - controlled study
KW - diet induced obesity
KW - fat tolerance test
KW - herbal tea
KW - high performance liquid chromatography
KW - Hypericum perforatum
KW - hypolipemia
KW - in vitro study
KW - infusion
KW - nonhuman
KW - obesity
KW - phytochemistry
KW - rat
KW - Salvia officinalis
KW - triacylglycerol blood level
PB - Birkhauser Boston
SN - 10542523 (ISSN)
LA - English
J2 - Med. Chem. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 54; Correspondence Address: R.
Reynoso-Camacho; Research and Graduate Studies in Food Science, School of Chemistry,
Universidad Autonoma de Queretaro, Queretaro, 76010, Mexico; email:
rrcamachomx@yahoo.com.mx; CODEN: MCREE
ER -

TY - JOUR
AU - Mazaro, S.M.
AU - Fogolari, H.
AU - Wagner, A.
AU - Citadin, I.
AU - Santos, I.
TI - Potential of Calendula officinalis L. extracts in inducing phytoalexin synthesis and
fungistatic effect on Botrytis cinerea in vitro
ST - Potencial de extratos à base de Calendula officinalis L. na indução da síntese de
fitoalexinas e no efeito fungistático sobre Botrytis cinerea, in vitro
PY - 2013
T2 - Revista Brasileira de Plantas Medicinais
VL - 15
IS - 2
SP - 208
EP - 216
DO - 10.1590/S1516-05722013000200007
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879316009&doi=10.1590%2fS1516-05
722013000200007&partnerID=40&md5=b8261b8d612c105c572b8c13b6e0eac5
AD - Universidade Tecnológica Federal do Paraná (UTFPR), CEP:85.660-000, Dois Vizinhos,
PR, Campus Dois Vizinhos, Estrada para Boa Esperanca, Km 4, Brazil
AD - UTFPR, CEP:85.501-970, Pato Branco, PR, Campus Pato Branco, Via do Conhecimento,
Caixa Postal 571, Brazil
AB - Three experiments were carried out to evaluate the potential of calendula (Calendula
officinalis L.) extracts for phytoalexin induction in soybean cotyledons, resistance mechanism
induction in strawberry fruits and fungistatic effect on Botrytis cinerea in vitro. Experimental
design was completely randomized with 15 treatments resulting from the combination of three
forms of extraction (alcohol extract, infusion and maceration) at five concentrations (zero, 1.25,
2.5, 5 and 10%). Results showed that C. officinalis extracts could induce the phytoalexins
glyceollins in soybean cotyledons. In the application of extracts during the strawberry
postharvest, there was a change in flavonoid content, as well as in the activity of the enzyme
PAL, which was stimulated by the application of extracts; however, fruit rot control was not
noted. Fungistatic effect was observed for the extract obtained by maceration at all used
concentrations, reducing in vitro the growth of the fungus B. cinerea, and from 2.5% there was
total inhibition. The extract obtained by infusion also showed a positive response in reducing B.
cinerea growth, with better response at the concentration of 10% extract.
KW - Alternative control of diseases
KW - Glyceollin
KW - Medicinal plants
KW - Strawberry
KW - Botryotinia fuckeliana
KW - Calendula
KW - Calendula officinalis
KW - Fragaria x ananassa
KW - Fungi
KW - Glycine max
KW - alcohol
KW - flavonoid
KW - phytoalexin
KW - antifungal activity
KW - article
KW - Botrytis cinerea
KW - Calendula officinalis
KW - fruit
KW - growth inhibition
KW - in vitro study
KW - nonhuman
KW - postharvest period
KW - protein synthesis
KW - soybean
KW - strawberry
PB - Instituto de Biociencias
SN - 15160572 (ISSN)
LA - English
J2 - Rev. Bras. Plantas Med.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 8; Correspondence Address: S. M. Mazaro;
Universidade Tecnológica Federal do Paraná (UTFPR), CEP:85.660-000, Dois Vizinhos, PR,
Campus Dois Vizinhos, Estrada para Boa Esperanca, Km 4, Brazil; email: sergio@utfpr.edu.br;
CODEN: RBPMF
ER -

TY - JOUR
AU - Cruceriu, D.
AU - Balacescu, O.
AU - Rakosy, E.
TI - Calendula officinalis: Potential Roles in Cancer Treatment and Palliative Care
PY - 2018
T2 - Integrative Cancer Therapies
VL - 17
IS - 4
SP - 1068
EP - 1078
DO - 10.1177/1534735418803766
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065349361&doi=10.1177%2f15347354
18803766&partnerID=40&md5=2e71ad21721cf4e67d2a92593fe9982e
AD - The Oncology Institute “Prof. Dr. Ion Chiricuta, Cluj-Napoca, Romania
AD - Babes-Bolyai” University, Cluj-Napoca, Romania
AD - University of Medicine and Pharmacy “Iuliu Hatieganu,”, Cluj-Napoca, Romania
AB - A continuous challenge in cancer management is to improve treatment efficacy and to
diminish its side effects. Consequently, new conventional and unconventional drugs and
bioactive compounds from plants are constantly developed, characterized, and used for in vitro
and in vivo models. This review focuses on the antitumor properties of Calendula officinalis, its
biological and molecular effects in tumor cells and animal models, as well as its role in cancer
palliative care. A systematic review of studies describing the cytotoxic role of C officinalis and its
therapeutic role on cancer cells were carried out using the PubMed database. Albeit C officinalis
extracts have cytotoxic activity toward different cancer cell lines, a high grade of variation
between studies was observed, depending on plant organ subjected to extraction, extraction
method, and the cancer cell lines used for each study. Nevertheless, its cytotoxic activity is
related to a few bioactive compounds, presenting multiple roles in both activation of
proapoptotic proteins and decreasing the expression of the proteins that inhibit cell death.
Moreover, due to its anti-genotoxic/protective as well as antitumor and antimetastatic effects
proven in animal models, C officinalis could have important future implications in developing
novel cancer treatment strategies, while until now it has been used especially for diminishing the
side effects of radiotherapy. © The Author(s) 2018.
KW - antimetastatic
KW - Calendula
KW - cancer
KW - cytotoxicity
KW - genoprotective
KW - palliative care
KW - Animals
KW - Antineoplastic Agents
KW - Apoptosis
KW - Calendula
KW - Humans
KW - Neoplasms
KW - Palliative Care
KW - Phytotherapy
KW - Plant Extracts
KW - antimetastatic agent
KW - antineoplastic agent
KW - Calendula officinalis extract
KW - plant extract
KW - unclassified drug
KW - antineoplastic agent
KW - plant extract
KW - animal cell
KW - animal model
KW - antineoplastic activity
KW - Calendula officinalis
KW - cancer cell line
KW - cancer palliative therapy
KW - cell death
KW - cytotoxicity
KW - in vitro study
KW - in vivo study
KW - metastasis inhibition
KW - nonhuman
KW - priority journal
KW - Review
KW - systematic review
KW - tumor cell
KW - animal
KW - apoptosis
KW - Calendula
KW - chemistry
KW - drug effect
KW - human
KW - neoplasm
KW - palliative therapy
KW - phytotherapy
KW - procedures
PB - SAGE Publications Inc.
SN - 15347354 (ISSN)
C2 - 30289008
LA - English
J2 - Integr. Cancer Ther.
M3 - Review
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 30; Correspondence Address: O. Balacescu;
Department of Functional Genomics, Proteomics and Experimental Pathology, The Oncology
Institute “Prof. Dr. Ion Chiricuta,” 34-36 Republicii Street, Cluj-Napoca, 400015, Romania; email:
ovidiubalacescu@iocn.ro; CODEN: ICTNA
ER -

TY - JOUR
AU - Sharma, D.B.
AU - Aphale, P.
AU - Gandhi, V.
AU - Chitlange, S.S.
AU - Thomas, A.
TI - Qualitative analysis of calendula officinalis homeopathic mother tincture with the help of
high performance thin layer chromatography
PY - 2020
T2 - Research Journal of Pharmacy and Technology
VL - 13
IS - 3
SP - 1113
EP - 1116
DO - 10.5958/0974-360X.2020.00204.8
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086162097&doi=10.5958%2f0974-360
X.2020.00204.8&partnerID=40&md5=76391b81fe2b82dc774d74903e6c8702
AD - Department of Forensic Medicine & Toxicology, Dr. D.Y. Patil Homoeopathic Medical
College & Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
AD - Department of Homoeopathic Pharmacy, Dr. D.Y. Patil Homoeopathic Medical College &
Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
AD - Department of Practice of Medicine, Dr. D.Y. Patil Homoeopathic Medical College &
Research Centre, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India
AD - Dr. D.Y. Patil Institute of Pharmaceutical Sciences & Research, Dr. D.Y. Patil Unitech
Society, Pimpri, Pune, India
AD - Department of Pharmaceutical Chemistry, Dr. D.Y. Patil Institute of Pharmaceutical
Sciences & Research, Dr. D.Y. Patil Unitech Society, Pimpri, Pune, India
AB - Background: Chromatography is useful in qualitative and quantitative analysis of drugs
especially mother tinctures and lower potencies. Thin layer chromatography (TLC) and paper
chromatography (PC) are usually employed in the H.P.I. assays and tests. Thin layer
chromatography (TLC) is a simple, quick, and inexpensive procedure that gives a quick answer
as to how many components are in a mixture. TLC is also used to support the identity of a
compound in a mixture when the Rf of a known compound (preferably both run on the same
TLC plate). Materials & Methods: 1Standard Calendula Officinalis mother tincture was tested
against a pharmaceutical preparation of mother tincture of the same. 2 It was analysed for its
components using HPTLC. 3 Stationary phase consisted of TLC Aluminium sheets with silica
gel 60 F253 pre-coated layer (20cm x 10cm), thickness-0.2mm, no. of tracks-18, band
length-6mm. 4 Mobile Phase consisted of Chloroform:Methanol (9.5:0.5). 5 The plate was
developed in developing chamber and observed under U.V. Light. Results: 1 Colours seen on
the HPTLC Plates of samples are pink/violet which corresponds to amino acids, green which
corresponds to sterols, dark blue which corresponds to saponins, purple which corresponds to
triterpenoids and orange which corresponds to alkaloids. 2 The spectra analysis of standard and
sample shows equivalent peak/rise and fall in the spectra at exactly equivalent wavelengths.
Conclusion: Therapeutic action of Calendula Officinalis as noted in Homoeopathic Materia
Medica is because of the active principles like saponins, alkaloids, triterpenoudsaponins, sterols
and amino acids present it which is evident from the chromatogram. © RJPT All right reserved.
KW - Active principles
KW - HPTLC
KW - Qualitative and quantitative analysis
KW - Retention factor
KW - Thin Layer Chromatography
KW - amino acid
KW - Calendula extract
KW - Calendula officinalis extract
KW - saponin
KW - sterol
KW - triterpenoid
KW - unclassified drug
KW - Article
KW - Calendula officinalis
KW - chromatography by mobile phase
KW - drug industry
KW - high performance thin layer chromatography
KW - homeopathy
KW - nonhuman
KW - qualitative analysis
KW - thin layer chromatography
KW - tincture
PB - Research Journal of Pharmacy and Technology
SN - 09743618 (ISSN)
LA - English
J2 - Res. J. Pharm. Technol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: P. Aphale;
Department of Homoeopathic Pharmacy, Dr. D.Y. Patil Homoeopathic Medical College &
Research Centre, Pune, Dr. D.Y. Patil Vidyapeeth, Pimpri, India; email:
parth.aphale@gmail.com
ER -

TY - CHAP
AU - Egeli, D.
TI - Calendula officinalis L.
PY - 2022
T2 - Novel Drug Targets with Traditional Herbal Medicines: Scientific and Clinical Evidence
SP - 49
EP - 72
DO - 10.1007/978-3-031-07753-1_4
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85143642470&doi=10.1007%2f978-3-03
1-07753-1_4&partnerID=40&md5=7dcd0489923d081a41b57f34941873f0
AD - Faculty of Pharmacy, Department of Pharmacognosy, Bezmialem Vakif University,
Istanbul, Turkey
AB - Calendula officinalis, commonly known as “marigold,” is a plant with yellow-orange flowers
belonging to the Asteraceae family. The plant, which has been used since ancient times, is
widely used in both traditional and homeopathic medicine for the treatment of various diseases.
This chapter firstly summarized the description, distribution, and chemical composition of the
plant. In vivo, in vitro, and clinical studies on the plant have been shown in detail. In addition,
toxicity and mutagenicity studies have been reported. © The Editor(s) (if applicable) and The
Author(s), under exclusive license to Springer Nature Switzerland AG 2022.
KW - Biological activities
KW - Calendula officinalis L
KW - Chemical composition
KW - Traditional use
PB - Springer International Publishing
SN - 978-303107753-1 (ISBN); 978-303107752-4 (ISBN)
LA - English
J2 - Novel Drug Targets with Traditional Herb. Medicines: Scientific and Clinical Evid.
M3 - Book chapter
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1
ER -

TY - JOUR
AU - Diego, M.P.D.
TI - Homeopathic mother tinctures from Calendula officinalis and Echinacea angustifolia as oral
antiseptics
ST - Las tinturas madre homeopáticas de Calendula officinalis y Echinacea angustifolia como
antiséptico oral
PY - 2013
T2 - Revista Medica de Homeopatia
VL - 6
IS - 3
SP - 112
EP - 126
DO - 10.1016/j.homeo.2013.07.005
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891090543&doi=10.1016%2fj.homeo.2
013.07.005&partnerID=40&md5=1d606128f4464a97645125a72f76642e
AD - Directora de Dental Palacio, Santander, Spain
AB - The active components of the Calendula officinalis and Echinacea angustifolia plants are
perfectly preserved by the technique of making homeopathic mother tinctures. Thanks to these
components and their method of homeopathic dilution, they are highly efficient when used in the
oral cavity. This is due to their properties: analgesic, antiinflammatory, hemostatic, antibacterial,
antiviral, antifungal, immune stimulating, scar-healing and sialogogue. Their daily use in the
form of mouth wash decreases the appearance of cavities and prevents inflammatory processes
such as gingivitis, mucositis, periodontitis and peri-implantitis. Employed as the only treatment in
simple mouth surgery, they bring about rapid hemostasis, analgesia, absence of inflammation
and infection, and much shorter scar healing time than in patients treated with allopathic
medications. They constitute effective treatment in mucosal pathology, scar healing for injuries,
sores, oral candidiasis and hyposialia secondary to medications. With only 2 homeopathic
medications, better results are achieved than with an entire battery of allopathic medications, at
a reasonable price and without any side effects. © 2013 Elsevier España, S.L.
KW - Calendula officinalis
KW - Cavities
KW - Echinacea angustifolia
KW - Gingivitis
KW - Mouthwash
KW - Mucositis
KW - Oral antiseptic
KW - Oral mucosa pathology
KW - Oral surgery
KW - Peri-implantitis
KW - Periodontal pockets
SN - 18888526 (ISSN)
LA - Spanish
J2 - Rev. Med. Homeopatia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: M.P.D. Diego;
Directora de Dental Palacio, Santander, Spain; email: mercedespalacio@ono.com
ER -

TY - JOUR
AU - Shafeie, N.
AU - Naini, A.T.
AU - Jahromi, H.K.
TI - Comparison of different concentrations of calendula officinalis gel on cutaneous wound
healing
PY - 2015
T2 - Biomedical and Pharmacology Journal
VL - 8
IS - 2
SP - 979
EP - 992
DO - 10.13005/bpj/850
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958959016&doi=10.13005%2fbpj%2f8
50&partnerID=40&md5=742619dbb68f4868a5e85c064c2f9439
AD - Department of Nursing, Islamic Azad University, Firuzabad Branch, Firuzabad, Iran
AD - Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz,
Iran
AD - Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
AB - Lots of biological dressings and indigenous medicines have been reported to possess
wound healing properties. Calendula officinalis (marigold) has many pharmacological properties.
It is used for the treatment of skin disorders, pain and also as a bactericide, antiseptic and
antiinflammatory (8). In this investigation, the effects of different concentrations of Calendula
officinalis gel on histological and biomechanical changes of skin are studied. Seventy-five
mature male rats were randomly divided into three groups (control, placebo, and treatment
group). Under sterile conditions, a 2×2-cm piece of cervical skin for histopathological groups
and a rectangular shape with a metal ruler from cervical to lumbar region for biomechanical
groups, were excised in each animal. Treatment group received a daily topical application of
5%, 7%, and 10% C.officinalis gel, the placebo group received a daily topical application of the
base gel, and the control group received no treatment during this experimental study. Fourteen
and 21 days later, the rats were euthanized and biopsies were taken from the site of the initial
incisions and samples were collected for histopathological and biomechanical investigation.
Histopathological and biomechanical restorations in the group treated with 7% gel were
significantly more than the placebo and control group. Upper and lower doses seem to be less
effective, although the reasons for this remain unclear.
KW - Calendula officinalis gel
KW - Histopathological and biomechanical restorations
KW - Medicines
KW - animal experiment
KW - animal tissue
KW - Article
KW - biomechanics
KW - Calendula officinalis
KW - comparative study
KW - controlled study
KW - gel
KW - histopathology
KW - incision
KW - investigative procedures
KW - male
KW - nonhuman
KW - rat
KW - skin injury
KW - wound healing
PB - Oriental Scientific Publishing Company
SN - 09746242 (ISSN)
LA - English
J2 - Biomed. Pharmacol. J.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 18
ER -
TY - JOUR
AU - Belal, A.
AU - Elanany, M.A.
AU - Raafat, M.
AU - Hamza, H.T.
AU - Mehany, A.B.M.
TI - Calendula officinalis Phytochemicals for the Treatment of Wounds Through Matrix
Metalloproteinases-8 and 9 (MMP-8 and MMP-9): In Silico Approach
PY - 2022
T2 - Natural Product Communications
VL - 17
IS - 5
DO - 10.1177/1934578X221098848
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130284356&doi=10.1177%2f1934578X
221098848&partnerID=40&md5=ff80dcc7c334de45b6f13c860702fc25
AD - Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box
11099, Taif, 21944, Saudi Arabia
AD - School of Pharmacy and Pharmaceutical Industries, Badr University in Cairo (BUC), Cairo,
Egypt
AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Umm Al-Qura
University, Makkah, 21955, Saudi Arabia
AD - Biology Department, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi
Arabia
AD - Department of Zoology, Faculty of Science (Girls), Al-Azhar University, Yousef Abbas Str,
Cairo, Nasr City, Egypt
AD - Department of Zoology, Faculty of science (Boys), Al-Azhar University, Cairo, Nasr City,
Egypt
AB - Diabetic foot ulceration is a common complication of an uncontrolled diabetic regimen and
is considered a serious type of wound. Matrix metalloprteinases (MMPs) are the common key
enzymes in wound management, overexpression of MMPs can lead to chronic wounds and
ulcers. Calendula officinalis extract has established its efficacy in treating wounds in folk
medicine. In this research work, we will focus on the chemical constituents of this promising
herb and will investigate its abilities to target matrix metalloprteinase-8 (MMP-8) and matrix
metalloprteinase-9 (MMP-9) proteins through the usage of computer-aided drug design tools. In
the current study, several promising dual inhibitors are identified, such as quercetin,
isoquercetin, isorhamnetin, and isorhamnetin 3-O glycoside, they showed to be good inhibitors
for both enzyme subtypes with greater docking score energies than RND-336, which has been
reported as a selective MMP-9 inhibitor. Binding scores and amino acid interactions in addition
to molecular dynamics (MD) will be discussed in detail through this research work. © The
Author(s) 2022.
KW - Calendula
KW - DFU
KW - MMP8
KW - MMP9
KW - molecular docking
KW - molecular dynamics
KW - wounds
KW - alpha cadinol
KW - alpha tocopherol
KW - calendoflavoside
KW - Calendula extract
KW - Calendula officinalis extract
KW - calenduloside e
KW - cornulacic acid acetate
KW - cubenol
KW - erythrodiol
KW - esculetin
KW - gelatinase B
KW - glycoside
KW - isoquercetin
KW - isorhamnetin
KW - linoleic acid
KW - lupeol
KW - neutrophil collagenase
KW - phytochemical
KW - phytomenadione
KW - plastoquinone
KW - quercetin
KW - rutoside
KW - scopoletin
KW - ubiquinone
KW - umbelliferone
KW - unclassified drug
KW - wound healing promoting agent
KW - Article
KW - Calendula officinalis
KW - computer aided design
KW - drug screening
KW - enzyme structure
KW - molecular docking
KW - molecular dynamics
KW - molecular mechanics
KW - nonhuman
KW - protein binding
KW - protein conformation
KW - wound healing
PB - SAGE Publications Inc.
SN - 1934578X (ISSN)
LA - English
J2 - Nat. Pro. Comm.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 10; Correspondence Address: A. Belal;
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, P.O. Box
11099, 21944, Saudi Arabia; email: abilalmoh1@yahoo.com; M.A. Elanany; School of
Pharmacy and Pharmaceutical Industries, Badr University in Cairo (BUC), Cairo, Egypt; email:
mohamed.a.elanany@hotmail.com; A.B.M. Mehany; Department of Zoology, Faculty of science
(Boys), Al-Azhar University, Nasr City, Cairo, Egypt; email: abelal_81@azhar.edu.eg
ER -

TY - JOUR
AU - Shivasharan, B.D.
AU - Nagakannan, P.
AU - Thippeswamy, B.S.
AU - Veerapur, V.P.
AU - Bansal, P.
AU - Unnikrishnan, M.K.
TI - Protective effect of Calendula officinalis Linn. flowers against 3-nitropropionic acid induced
experimental Huntington's disease in rats
PY - 2013
T2 - Drug and Chemical Toxicology
VL - 36
IS - 4
SP - 466
EP - 473
DO - 10.3109/01480545.2013.776583
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883354802&doi=10.3109%2f01480545
.2013.776583&partnerID=40&md5=4e9654deb4ebd7a5da86070127ceb2cb
AD - Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumkur 572 102,
Karnataka, B.H. Road, India
AD - Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal
University, Manipal, Karnataka, India
AB - Oxidative stress (OS) and nitric oxide mechanisms have been recently proposed in
3-nitropropionic acid (3-NP)-induced neurotoxicity. The compounds, having antioxidant,
anti-inflammatory and estrogenic effects, have been suggested for neuroprotection in different
experimental models. Calendula officinalis Linn. flower extract (COE) is known for its potent
antioxidant, anti-inflammatory, estrogenic and neuroprotective activities. Hence, the present
study was designed to evaluate the neuroprotective effect of COE on 3-NP-induced
neurotoxicity in rats by observing behavioral changes, OS and striatal damage in rat brain. Adult
female Wistar rats were pretreated with vehicle or COE (100 and 200mg/kg) for 7 days, followed
by cotreatment with 3-NP (15mg/kg, intraperitoneally) for the next 7 days. At the end of the
treatment schedule, rats were evaluated for alterations in sensory motor functions and
short-term memory. Animals were sacrificed and brain homogenates were used for the
estimation of lipid peroxidation (LPO), glutathione, total thiols, glutathione S-transferase,
catalase and nitrite. A set of brain slices was used for the evaluation of neuronal damage in the
striatal region of the brain. 3-NP caused significant alterations in animal behavior, oxidative
defense system evidenced by raised levels of LPO and nitrite concentration, and depletion of
antioxidant levels. It also produced a loss of neuronal cells in the striatal region. Treatment with
COE significantly attenuated behavioral alterations, oxidative damage and striatal neuronal loss
in 3-NP-treated animals. The present study shows that COE is protective against 3-NP-induced
neurotoxicity in rats. The antioxidant, anti-inflammatory and estrogenic properties of COE may
be responsible for its neuroprotective action. © 2013 Informa Healthcare USA, Inc. All rights
reserved.
KW - Excitotoxicity
KW - Marigold
KW - Neuroprotective
KW - Nitrative stress
KW - Oxidative stress
KW - TTC
KW - Analysis of Variance
KW - Animals
KW - Body Weight
KW - Calendula
KW - Chromatography, High Pressure Liquid
KW - Disease Models, Animal
KW - Female
KW - Flowers
KW - Huntington Disease
KW - Locomotion
KW - Maze Learning
KW - Nitro Compounds
KW - Plant Extracts
KW - Propionates
KW - Rats
KW - Rats, Wistar
KW - 3 nitropropionic acid
KW - antiinflammatory agent
KW - antioxidant
KW - Calendula extract
KW - catalase
KW - glutathione
KW - glutathione transferase
KW - neuroprotective agent
KW - nitrite
KW - thiol derivative
KW - animal behavior
KW - animal experiment
KW - animal model
KW - animal tissue
KW - antiinflammatory activity
KW - antioxidant activity
KW - article
KW - brain homogenate
KW - Calendula officinalis
KW - controlled study
KW - female
KW - flower
KW - Huntington chorea
KW - lipid peroxidation
KW - neuroprotection
KW - neurotoxicity
KW - nonhuman
KW - randomized controlled trial
KW - rat
KW - sensorimotor function
KW - short term memory
SN - 15256014 (ISSN)
C2 - 23590827
LA - English
J2 - Drug Chem. Toxicol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 30; Correspondence Address: B.S.
Thippeswamy; Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumkur
572 102, Karnataka, B.H. Road, India; email: t_swamy@hotmail.com; CODEN: DCTOD
ER -

TY - JOUR
AU - da Silva, L.M.
AU - Araújo, A.C.
AU - Bunn, V.S.
AU - Mariano, L.N.B.
AU - Somensi, L.B.
AU - Boeing, T.
AU - Klein, L.C.
AU - de Andrade, S.F.
TI - Calendula officinalis L. Inflorescences extract: In vivo evaluation of its gastric ulcer healing
potential
ST - Extrato de inflorescências de Calendula officinalis L.: avaliação in vivo do seu potencial
como cicatrizante de úlcera gástrica
PY - 2020
T2 - Biomedical and Biopharmaceutical Research
VL - 17
IS - 1
SP - 1
EP - 15
DO - 10.19277/bbr.17.1.230
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085112079&doi=10.19277%2fbbr.17.1.
230&partnerID=40&md5=a83b30544f6d2a2f9ccc0e7a6f50e970
AD - Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Núcleo de
Investigacões Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI),
Rua Uruguai, 458, Centro, Itajaí, 88302-202, SC, Brazil
AD - Graduação em Nutrição, Universidade do Vale do Itajaí (UNIVALI), Campus Itajaí, Rua
Uruguai, 458, Santa Catarina, CEP: 88302-901, Brazil
AD - Graduação em Biomedicina, Universidade do Vale do Itajaí (UNIVALI), Campus Itajaí,
Rua Uruguai, 458, Santa Catarina, CEP: 88302-901, Brazil
AD - Universidade Lusófona - CBIOS - Research Center for Biosciences and Health
Technologies, Av Campo Grande, 376, Lisboa, 1749-024, Portugal
AB - Calendula officinalis L. (Asteraceae), popularly known as Marigold in Europe and
“Calêndula” in Brazil is a medicinal plant used for centuries to treat a wide variety of ailments
including gastric ulcers. Despite its folk use, there are few studies about the potential of C.
officinalis extract to heal gastric ulcers. In this study, gastric ulcer healing potential of C.
officinalis inflorescences (HECO) was evaluated using chronic gastric ulcer induced by acetic
acid in Wistar rats. Different groups (n=6) were treated orally with vehicle (water plus 0.5%
Tween 80, 1 mL/100 g), omeprazole (20 mg/ kg), or HECO (10, 30 and 100 mg/kg), twice a day
for seven days. The results showed that HECO is rich in phenolic substances and displays
healing chronic gastric ulcer activity, evidenced by diminished lesion area. Additionally, the data
suggest that its effectiveness is related, at least in part, to the capacity of extract to increase
production of protective factors of gastric mucosa, such as mucus and antioxidants, and to
increase collagen synthesis. This study contributes to the validation of the folk use of the extract
of C. officinalis inflorescences for the treatment of gastric ulcer. Copyright © 2020 ALIES. All
Rights Reserved.
KW - Calendula
KW - Gastric ulcer
KW - Marigold
KW - Ulcer healing
KW - alanine aminotransferase
KW - antiulcer agent
KW - Calendula extract
KW - Calendula officinalis extract
KW - collagen
KW - glutathione
KW - omeprazole
KW - stomach mucin
KW - unclassified drug
KW - alanine aminotransferase blood level
KW - animal experiment
KW - animal model
KW - animal tissue
KW - antioxidant activity
KW - antiulcer activity
KW - Article
KW - Calendula officinalis
KW - collagen synthesis
KW - controlled study
KW - drug screening
KW - in vivo study
KW - inflorescence
KW - male
KW - nonhuman
KW - rat
KW - stomach mucosa
KW - stomach mucus
KW - stomach ulcer
KW - ulcer healing
KW - ulcer index
PB - ALIES
SN - 21822360 (ISSN)
LA - English
J2 - Biomed. Biopharm. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2; Correspondence Address: S.F. de Andrade;
Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Núcleo de Investigacões
Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Rua
Uruguai, 458, Centro, 88302-202, Brazil; email: sergio.andrade@ulusofona.pt
ER -
TY - JOUR
AU - Chaleshtori, S.H.
AU - Kachoie, M.A.
AU - Pirbalouti, A.G.
TI - Chemical composition and antimicrobial effects of Calendula officinalis grown under
chemical and biological conditions on the methicillin-resistant Staphylococcus aureus isolated
from hospital infections
PY - 2016
T2 - Biosciences Biotechnology Research Asia
VL - 13
IS - 3
SP - 1787
EP - 1796
DO - 10.13005/bbra/2331
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84991661595&doi=10.13005%2fbbra%2f
2331&partnerID=40&md5=6549358ead15af98c168bc3417d7d509
AD - Department of Herbal Medicine, College of Food and Drug, Shahrekord Branch, Islamic
Azad University, Shareakord, Iran
AB - Application of various types of fertilizers can effect on biological activities of Calendula
officinalis. The present investigation was aimed to study the chemical components and
antimicrobial effects of C. officinalis grown under chemical and biological conditions on
methicillin-resistant Staphylococcus aureus. Four hundred samples of hospital infections were
collected and cultured. MRSA strains were subjected to the disk diffusion and GC-Mass.
One-hundred out of 400 samples of hospital infections were positive for MRSA (25%). All
isolates were also positive for mecA gene. Forty different chemical components were detected
in the C. officinalis. The most variable components were found in the control group (1,8-cineole
(30.456%), ã-terpinene (25.547%), terpinolene (4.584%), á-terpineol (4.490%) and
trans-â-ocinene (4.153%)). Application of biologic and chemical fertilizers caused significant
increase in the levels of some chemical components (P < 0.05). MRSA strains harbored the
highest levels of resistance against tetracycline (95%), ampicillin (92%), penicillin (90%),
gentamycin (88%) and ciprofloxacin (77%). Control group had the highest antimicrobial effects
but essential oil of the C. officinalis enriched with both fertilizers were effective on resistant
MRSA. Use of C. officinalis growth under both chemical and biologic fertilizers has been
recommended as a primary approach for synthesis of effective antibiotic.
KW - Antimicrobial effects
KW - Biologic fertilizer
KW - Calendula officinalis
KW - Chemical components
KW - Chemical fertilizer
KW - Methicillin resistant Staphylococcus aureus
KW - alpha thujene
KW - ampicillin
KW - azithromycin
KW - bisabolol
KW - bourbonene
KW - Calendula officinalis extract
KW - camphene
KW - cefixime
KW - cineole
KW - ciprofloxacin
KW - clindamycin
KW - copaene
KW - cotrimoxazole
KW - gamma cadinene
KW - gentamicin
KW - imipenem
KW - lincomycin
KW - ocimene
KW - penicillin binding protein 2a
KW - penicillin derivative
KW - pinene
KW - plant extract
KW - spathulenol
KW - T cadinol
KW - terpinene
KW - terpineol
KW - terpinolene
KW - tetracycline
KW - unclassified drug
KW - unindexed drug
KW - vegetable oil
KW - antibiotic sensitivity
KW - antimicrobial activity
KW - Article
KW - bacterium identification
KW - Calendula officinalis
KW - chemical composition
KW - controlled study
KW - disk diffusion
KW - fertilizer application
KW - flower
KW - gene identification
KW - hospital infection
KW - human
KW - inhibition zone
KW - major clinical study
KW - mass fragmentography
KW - methicillin resistant Staphylococcus aureus
KW - nonhuman
KW - polymerase chain reaction
PB - Oriental Scientific Publishing Company
SN - 09731245 (ISSN)
LA - English
J2 - Biosci. Biotechnol. Res. Asia
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: M.A. Kachoie;
Department of Herbal Medicine, College of Food and Drug, Shahrekord Branch, Islamic Azad
University, Shareakord, Iran; email: Mehrdad.ataie@gmail.com
ER -

TY - JOUR
AU - Maie Mohsen, M.A.
AU - Ismail, H.
TI - Response of Calendula officinalis L. which grown in saline soil to plant growth promoters
and some organic substances
PY - 2016
T2 - International Journal of PharmTech Research
VL - 9
IS - 4
SP - 153
EP - 172
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84969213650&partnerID=40&md5=6192
d013b5192ac4ea6193c379c2535a
AD - Medicinal and Aromatic plants, Res. Dept., Hort. Res. Inst., A. R. C., Dokky, Giza, Egypt
AD - Agric. Microbial. Res. Dept., Soil, Water and Environ, Res. Inst. A.R.C., Giza, Egypt
AB - Two factorial experiments in split plot design were carried out during the two successive
growing seasons of 2011/2012 and 2012/2013 at the farm of Soils, Water and Environ. Res.
Inst., Agric., Res. Center in Sahl El-Hossynia, EL-Sharkia Governorate; Egypt to investigate
effects of some salinity tolerant,[Azotobacter chroococcum, Azopirillum lipoferum, Bacillus
polymyxa, Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas fluorescens and the white rot
fungus(Pleurotus columbines)] as well as plant growth promoters (PGP) alone or with different
organic treatments as Cattle manure (CM) at 20m3/fed., ascorbic acid (ASC) at 100 ppm, Humic
acid (HA) at 100 ppm as foliar spray and their interactions under saline sodic soil conditions on
Marigold (Calendula officinalis L.) vegetative growth, flowering growth, chemical analysis and
chemical properties of soil. Gradual and significant increases in plant height (cm), number of
branches, leaves and inflorescences /plant, leaf area of medium leaf of main stem and fresh
and dry weights of shoots, roots and inflorescences per plant (g), diameter of inflorescences
(cm) and chemical analysis as, chlorophyll (A&B), total carotenoids in leaves and ray flowers,
total carbohydrates and N,P,K percentage in leaves were recorded increasing with the tested
treatments especially CM+ ASC+HA with (PGP) followed by that the treatment CM+HA with
(PGP) and CM+ASC with (PGP) respectively. While these treatments decrease the proline
content in fresh leaves, Ph and EC of the soil, Nitrogenase and dehydrogenase in soil were
enhanced by all tested treatments specially in case of organic matter treatments with PGP
which reflected on the quality and quantity of marigold plants. © 2016, Sphinx Knowledge
House. All rights reserved.
KW - Ascorbic acid
KW - Calendula officinalis
KW - Himic acid
KW - Marigold
KW - PGP
KW - Saline soil
KW - ascorbic acid
KW - carotenoid
KW - chlorophyll
KW - growth promotor
KW - humic acid
KW - nitrogenase
KW - oxidoreductase
KW - phytohormone
KW - proline
KW - sodium chloride
KW - soil organic matter
KW - Article
KW - Azospirillum lipoferum
KW - Azotobacter chroococcum
KW - Bacillus subtilis
KW - Calendula officinalis
KW - chlorophyll content
KW - controlled study
KW - factorial design
KW - field experiment
KW - flowering
KW - Klebsiella pneumoniae
KW - leaf area
KW - manure
KW - nonhuman
KW - Paenibacillus polymyxa
KW - phytochemistry
KW - plant height
KW - Pseudomonas fluorescens
KW - rhizosphere bacterium
KW - salinity
KW - soil property
KW - soil treatment
KW - vegetative growth
KW - white rot fungus
PB - Sphinx Knowledge House
SN - 09744304 (ISSN)
LA - English
J2 - Int. J. Pharm. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 3
ER -

TY - JOUR
AU - Tripathi, A.
AU - Tyagi, S.
AU - Singh, G.
AU - Ghosh, K.
AU - Gupta, A.
TI - Exploration its antioxidant and immunogenic potential of protein: Calendula officinalis and
dianthus chinensis
PY - 2021
T2 - Research Journal of Pharmacy and Technology
VL - 14
IS - 6
SP - 2989
EP - 2994
DO - 10.52711/0974-360X.2021.00523
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113382172&doi=10.52711%2f0974-36
0X.2021.00523&partnerID=40&md5=456c413921d371d863b9864d5b01e9c6
AD - Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, Clement
Town, India
AB - Aim and objective: Pollen is reported in flowers and leaves of various medicinal plants and
showed its beneficial effect on human health. The objective of our study is to evaluate the
antioxidant effect of flowers (Calendula officinalis and Dianthus chinensis) especially pollen
protein and also determining its immunogenic effect against specific protein (typhoid vaccine)
antigen in human whole blood samples. Methods: In this study, analytical studies were
conducted for estimating the protein content using Lowry test and qualitatively determined
through high performance thin layer chromatography (HPLC). In addition, immunological studies
were also conducted using pollen protein for evaluating its antioxidant potential (using DPPH
(2,2-diphenyl-1picryl-hydrazyl-hydrate) free radical assay) and determined its immunogenic
effect using typhoid vaccine antigen and determining its hemolytic effect in human whole blood
samples. Results: This work demonstrated that pollen protein content (determined through lowry
test) at higher concentrations exhibited a significant declining in antigen specific T cell
proliferation using optimized dose of typhoid vaccine antigen in human whole blood samples. In
addition, pollen protein showed antioxidant property as well and does not show any hemolytic
effect. Conclusion: The current study showed that pollen protein from Calendula officinalis and
Dianthus chinensis possesses antioxidant property and less immunogenic in terms of declining
in antigen specific T cell proliferation rate. © RJPT All right reserved.
KW - Antioxidant
KW - Calendula officinalis
KW - Dianthus chinensis
KW - Typhoid vaccine
KW - antigen
KW - Calendula officinalis extract
KW - Dianthus chinensis extract
KW - plant extract
KW - plant protein
KW - pollen protein
KW - typhoid vaccine antigen
KW - unclassified drug
KW - antioxidant assay
KW - Article
KW - blood sampling
KW - Calendula officinalis
KW - cell proliferation
KW - cell viability
KW - controlled study
KW - Dianthus chinensi
KW - DPPH radical scavenging assay
KW - extraction
KW - hemolysis
KW - high performance liquid chromatography
KW - human
KW - human cell
KW - immune response
KW - immunogenicity
KW - medicinal plant
KW - MTT assay
KW - organoleptic property
KW - oxidative stress
KW - phytochemistry
KW - plant identification
KW - protein analysis
KW - protein content
KW - protein function
KW - quality control
KW - spectrophotometry
KW - traditional medicine
PB - Research Journal of Pharmacy and Technology
SN - 09743618 (ISSN)
LA - English
J2 - Res. J. Pharm. Technol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2; Correspondence Address: A. Gupta;
Department of Biotechnology, Graphic Era (Deemed to be) University, Clement Town,
Dehradun, India; email: dr.amitgupta.bt@geu.ac.in
ER -

TY - JOUR
AU - Lima, M.R.
AU - Lopes, A.P.
AU - Martins, C.
AU - Brito, G.A.C.
AU - Carneiro, V.C.
AU - Goes, P.
TI - The effect of Calendula officinalis on oxidative stress and bone loss in experimental
periodontitis
PY - 2017
T2 - Frontiers in Physiology
VL - 8
IS - JUN
C7 - 440
DO - 10.3389/fphys.2017.00440
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021654598&doi=10.3389%2ffphys.201
7.00440&partnerID=40&md5=ceb8cd40559998d5bc0fe7a7a1ab06c3
AD - Nucleus of Study and Research in Pain, Inflammation, and Osteoimmunology, Department
of Morphology, Medical School, Federal University of Ceará, Fortaleza, Brazil
AD - Nucleus of Study and Research in Pain, Inflammation, and Osteoimmunology, Department
of Pathology and Legal Medicine, Medical School, Federal University of Ceará, Fortaleza, Brazil
AD - Department of Morphology, Medical School, Federal University of Ceará, Fortaleza, Brazil
AB - Periodontitis is associated with reduced antioxidant capacity and increased oxidative
damage. Oxidative stress induces inflammation and bone loss contributing to the pathological
progression of periodontal disease. Calendula officinalis (CLO) has demonstrated
anti-inflammatory and anti-oxidant activities. Therefore, the aim of this study was to evaluate the
effect of CLO on oxidative stress and bone loss in rats subjected to experimental periodontitis
(EP). For this, 72 male Wistar rats were divided into groups: Naïve, Saline (SAL) and CLO. Rats
received SAL or CLO (90 mg/kg) 30 min before ligature and daily until the 11th day. Naïve group
experienced no manipulation. After 11 days, the animals were euthanized and left maxillae
collected for macroscopic analysis of alveolar bone loss (ABL). Periodontium was analyzed by
macroscopy, scanning electron microscopy; confocal and light polarized microscopy.
Immunohistochemical examination of DKK1, WNT 10b and β-catenin was performed. The
gingival tissue was collected to reduced glutathione (GSH), superoxide dismutase (SOD),
catalase (CAT) and malondialdehyde (MDA) analyses. The 11 days of ligature induced bone
loss, breakdown of collagen fibers, increased the immunostaining DKK-1 while reduced WNT
10b and β-catenin expressions. Periodontitis reduced GSH, SOD, CAT and increase MDA. All
findings were reversed by 90 mg/kg of CLO. In summary our findings demonstrated that CLO
reduced oxidative stress and bone loss and preserved collagen fibers in rats with EP, with
participation of WNT signaling pathway. © 2017 Lima, Lopes, Martins, Brito, Carneiro and Goes.
KW - Bone loss
KW - Calendula officinalis
KW - Oxidative stress
KW - Periodontitis
KW - Rats
KW - beta catenin
KW - Calendula officinalis extract
KW - catalase
KW - dickkopf 1 protein
KW - glutathione
KW - malonaldehyde
KW - plant extract
KW - sodium chloride
KW - superoxide dismutase
KW - unclassified drug
KW - Wnt10b protein
KW - animal experiment
KW - animal model
KW - antiinflammatory activity
KW - antioxidant activity
KW - Article
KW - bone tissue
KW - Calendula officinalis
KW - collagen fiber
KW - confocal microscopy
KW - controlled study
KW - experimental periodontitis
KW - immunohistochemistry
KW - light polarized microscopy
KW - male
KW - microscopy
KW - nonhuman
KW - osteolysis
KW - oxidative stress
KW - protein expression
KW - rat
KW - scanning electron microscopy
KW - Wistar rat
PB - Frontiers Media S.A.
SN - 1664042X (ISSN)
LA - English
J2 - Front. Physiol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 24; Correspondence Address: P. Goes; Nucleus
of Study and Research in Pain, Inflammation, and Osteoimmunology, Department of Pathology
and Legal Medicine, Medical School, Federal University of Ceará, Fortaleza, Brazil; email:
paulagpinheiro@yahoo.com.br
ER -

TY - JOUR
AU - Abaas, I.S.
TI - Effect of biological competition of weeds on growth and volatile oil yield of marigold
(Calendula officinalis L.) as medicinal plant used in Herbal medicine of Iraq
PY - 2014
T2 - International Journal of Pharmacy and Pharmaceutical Sciences
VL - 6
IS - 1
SP - 217
EP - 219
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891940650&partnerID=40&md5=8dd1
54eb62ea12f3a1a972994daeedc9
AD - Department of Pharmacognosy and Medicinal plants, College of Pharmacy, University of
Kerbala, Iraq
AB - Objective: The study was designed to evaluation the effect of weed competition on growth
parameters and volatile oil of marigold flowers. Methods: The experiment was included four
treatments were weed free, weed infasted, black plastic mulch and white plastic mulch. The
samples for study were randomly collected from the middle rows of each plot. The volatile oil
was obtained by steam distillation. Results: The Results referred to all growth parameters of
marigold were reduced by weed competition copared with weed free treatment as control
treatment. Black plastic mulch was gave highest growth values than all treatment, and reached
to 25.8 plant -1, 41.25 cm2 plant-1, 25.40 plant-1, 25.10 plant -1 and 905 kgha -1 for basal
branches, leaf area, flower branches, number of inflorescences and yield of inflorescences
receptively. The highest value of volatile oil percentage and volatile oil yield were obtained at
black plastic mulch, and reached to 0.930% and 8.410 L ha-1 respectivly. Conclusion: From this
study the results and discussion, it has been concluded that weed removes is necessary to
prevent any significant yield losses of marigold. Plastic mulch may be considered effective weed
control tools and appropriate for medicinal plants.
KW - Calendula officicnalis
KW - Marigold
KW - Medicinal plants
KW - essential oil
KW - article
KW - Calendula officinalis
KW - chemical analysis
KW - controlled study
KW - flower
KW - herbal medicine
KW - inflorescence
KW - mulch
KW - nonhuman
KW - plant growth
KW - plant leaf
SN - 09751491 (ISSN)
LA - English
J2 - Int. J. Pharmcy Pharm. Sci.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: I. S. Abaas;
Department of Pharmacognosy and Medicinal plants, College of Pharmacy, University of
Kerbala, Iraq; email: ibrahim993@yahoo.com
ER -

TY - JOUR
AU - Hughes, R.R.
AU - Scown, D.
AU - Lenehan, C.E.
TI - Sequential injection analysis with chemiluminescence detection for rapid monitoring of
commercial Calendula officinalis extractions
PY - 2015
T2 - Phytochemical Analysis
VL - 26
IS - 6
SP - 454
EP - 460
DO - 10.1002/pca.2580
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84944414082&doi=10.1002%2fpca.2580
&partnerID=40&md5=8b30b535d62709257b3b32a6aba6e3f2
AD - Flinders University, School of Chemical and Physical Sciences, Bedford Park, 5042, SA,
Australia
AD - Jurlique International, Mount Barker, 5251, SA, Australia
AB - Introduction Plant extracts containing high levels of antioxidants are desirable due to their
reported health benefits. Most techniques capable of determining the antioxidant activity of plant
extracts are unsuitable for rapid at-line analysis as they require extensive sample preparation
and/or long analysis times. Therefore, analytical techniques capable of real-time or pseudo
real-time at-line monitoring of plant extractions, and determination of extraction endpoints,
would be useful to manufacturers of antioxidant-rich plant extracts. Objectives To develop a
reliable method for the rapid at-line extraction monitoring of antioxidants in plant extracts.
Materials and Methods Calendula officinalis extracts were prepared from dried flowers and
analysed for antioxidant activity using sequential injection analysis (SIA) with
chemiluminescence (CL) detection. The intensity of CL emission from the reaction of acidic
potassium permanganate with antioxidants within the extract was used as the analytical signal.
The SIA-CL method was applied to monitor the extraction of C. officinalis over the course of a
batch extraction to determine the extraction endpoint. Results were compared with those from
ultra high performance liquid chromatography (UHPLC). Results Pseudo real-time, at-line
monitoring showed the level of antioxidants in a batch extract of Calendula officinalis plateaued
after 100 min of extraction. These results correlated well with those of an offline UHPLC study.
Conclusion SIA-CL was found to be a suitable method for pseudo real-time monitoring of plant
extractions and determination of extraction endpoints with respect to antioxidant concentrations.
The method was applied at-line in the manufacturing industry. Copyright © 2015 John Wiley &
Sons, Ltd.
KW - chemiluminescence
KW - extraction monitoring
KW - Plant extract
KW - process analysis
KW - sequential injection analysis
KW - Antioxidants
KW - Calendula
KW - Chromatography, High Pressure Liquid
KW - Luminescence
KW - Plant Extracts
KW - Calendula officinalis plant extract
KW - permanganate potassium
KW - plant extract
KW - unclassified drug
KW - antioxidant
KW - plant extract
KW - antioxidant activity
KW - Article
KW - Calendula officinalis
KW - chemoluminescence
KW - drug isolation
KW - flower
KW - intermethod comparison
KW - sequential injection analysis
KW - ultra performance liquid chromatography
KW - Calendula
KW - chemistry
KW - evaluation study
KW - high performance liquid chromatography
KW - luminescence
KW - procedures
PB - John Wiley and Sons Ltd
SN - 09580344 (ISSN)
C2 - 26219427
LA - English
J2 - Phytochem. Anal.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2; CODEN: PHANE
ER -

TY - JOUR
AU - Băieş, M.-H.
AU - Gherman, C.
AU - Boros, Z.
AU - Olah, D.
AU - Vlase, A.-M.
AU - Cozma-Petruț, A.
AU - Györke, A.
AU - Miere, D.
AU - Vlase, L.
AU - Crișan, G.
AU - Spînu, M.
AU - Cozma, V.
TI - The Effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum
sativum L., Satureja hortensis L. and Calendula officinalis L. on the Embryogenesis of Ascaris
suum Eggs during an In Vitro Experimental Study
PY - 2022
T2 - Pathogens
VL - 11
IS - 9
C7 - 1065
DO - 10.3390/pathogens11091065
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138538666&doi=10.3390%2fpathogen
s11091065&partnerID=40&md5=7cee1e0042f6bdae41d986241f679fb6
AD - Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine,
University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 3–5 Mǎnǎştur
Street, Cluj-Napoca, 400372, Romania
AD - Department of Infectious Diseases, Faculty of Veterinary Medicine, University of
Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 3–5 Mǎnǎştur Street,
Cluj-Napoca, 400372, Romania
AD - Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Haţieganu” University
of Medicine and Pharmacy, 23 Gheorghe Marinescu Street, Cluj-Napoca, 400337, Romania
AD - Department of Bromatology, Hygiene, Nutrition, Faculty of Pharmacy, “Iuliu Haţieganu”
University of Medicine and Pharmacy, 6 Pasteur Street, Cluj-Napoca, 400349, Romania
AD - Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy,
“Iuliu Haţieganu” University of Medicine and Pharmacy, 12 Ion Creangǎ Street, Cluj-Napoca,
400010, Romania
AD - Academy of Agricultural and Forestry Sciences Gheorghe Ionescu-Siseşti (A.S.A.S), 61
Mǎrǎşti Boulevard, Bucharest, 011464, Romania
AB - Ascaris suum is present in traditionally managed indoor pig herds and in industrialized
farms, especially in older fatteners and sows. The increasing resistance to common
antihelminthic drugs redirected research towards alternative and traditional therapies, which
also include medicinal plants. This study comparatively evaluated the in vitro antiparasitic
effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum sativum L.,
Satureja hortensis L. and Calendula officinalis L. against A. suum egg hatching and larval
development. A. suum eggs were sampled from randomized fecal specimens collected from
traditionally raised swine. The egg suspension (ES, 12 × 103/mL) was divided into two controls
(C) (1C—1 mL ES + 1 mL distilled water, 2C—five plates of 1 mL ES + 1 mL ethanol of 70%,
35%, 17.5%, 8.75%, and 4.375%, respectively) and six experimental groups, and placed in 3
mL cell plates. The experimental groups (EG, 1–6) included ES + each alcoholic plant extract
(10%, 5%, 2.5%, 1.25%, 0.625%). Both C and EG were performed in quintuplicate. All variants
were incubated at 27 °C for a total of 21 days. A. suum eggs were examined after 2, 14 (L1),
and 21 (L2/L3) days of incubation. The efficacy of all tested plant extracts increased with
concentration. Anti-embryogenic effects on A. suum eggs were expressed by all plants. A
superior influence was observed in A. sativum L., A. absinthium L., C. pepo L. and S. hortensis
L. extracts, at all concentrations tested. A. sativum L. and A. absinthium L. extracts showed the
strongest antihelminthic activity, while C. sativum L. and C. officinalis L. were the weakest
ascaricids. Future in-depth phytochemical studies are required to identify the compounds
responsible for the anthelminthic properties of these plant species. © 2022 by the authors.
KW - antihelminthics
KW - Ascaris suum
KW - Romanian medicinal plants
KW - phytochemical
KW - plant extract
KW - animal experiment
KW - anthelmintic activity
KW - antiparasitic activity
KW - Artemisia absinthium
KW - Article
KW - Ascaris suum
KW - Calendula officinalis
KW - chemical analysis
KW - chemical composition
KW - controlled study
KW - coriander
KW - Cucurbita pepo
KW - egg
KW - embryo development
KW - enzyme linked immunosorbent assay
KW - female
KW - garlic
KW - high performance liquid chromatography
KW - larval development
KW - liquid chromatography-mass spectrometry
KW - male
KW - medicinal plant
KW - nonhuman
KW - oxygenation
KW - phytochemistry
KW - Satureja hortensis
PB - MDPI
SN - 20760817 (ISSN)
LA - English
J2 - Pathogens
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 5; Correspondence Address: A. Cozma-Petruț;
Department of Bromatology, Hygiene, Nutrition, Faculty of Pharmacy, “Iuliu Haţieganu”
University of Medicine and Pharmacy, Cluj-Napoca, 6 Pasteur Street, 400349, Romania; email:
anamaria.cozma@umfcluj.ro
ER -

TY - JOUR
AU - Babaee, N.
AU - Moslemi, D.
AU - Khalilpour, M.
AU - Vejdani, F.
AU - Moghadamnia, Y.
AU - Bijani, A.
AU - Baradaran, M.
AU - Kazemi, M.T.
AU - Khalilpour, A.
AU - Pouramir, M.
AU - Moghadamnia, A.A.
TI - Antioxidant capacity of calendula officinalis flowers extract and prevention of radiation
induced oropharyngeal mucositis in patients with head and neck cancers: A randomized
controlled clinical study
PY - 2013
T2 - DARU, Journal of Pharmaceutical Sciences
VL - 21
IS - 1
C7 - 18
DO - 10.1186/2008-2231-21-18
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876270713&doi=10.1186%2f2008-223
1-21-18&partnerID=40&md5=b1c2281617fc0689738bf8df2325574d
AD - Department of Oral Diseases, School of Dentistry, Babol University of Medical Sciences,
Babol, Iran
AD - Division of Radiation Oncology, School of Medicine, Babol University of Medical Sciences,
Babol, Iran
AD - School of Basic Sciences, Alzahra University, Tehran, Iran
AD - Non-Communicable Pediatric Disease Research Center, Babol University of Medical
Sciences, Babol, Iran
AD - Department of Pharmacology, School of Medicine, Babol University of Medical Sciences,
Babol, Iran
AD - Department of Biochemistry, School of Medicine, Babol University of Medical Sciences,
Babol, Iran
AB - This study was designed to determine the effect of Calendula officinalis flowers extract
mouthwash as oral gel on radiation-induced oropharyngeal mucositis (OM) in patients with
head-and-neck cancer. Forty patients with neck and head cancers under radiotherapy or
concurrent chemoradiotherapy protocols were randomly assigned to receive either 2%
calendula extract mouthwash or placebo (20 patients in each group). Patients were treated with
telecobalt radiotherapy at conventional fractionation (200 cGy/fraction, five fractions weekly,
30-35 fractions within 4-7 weeks). The oropharyngeal mucositis was evaluated by two clinical
investigators (a radiation oncologist and a dentist), using the oral mucositis assessment scale
(OMAS). Trying to find out the possible mechanism of action of the treatment, total antioxidant,
polyphenol and flavonoid contents, and quercetin concentration of the mouth wash were
measured. Calendula mouthwash significantly decreased the intensity of OM compared to
placebo at week 2 (score: 5.5 vs. 6.8, p = 0.019), week 3 (score: 8.25 vs. 10.95, p < 0.0001)
and week 6 (score: 11.4 vs. 13.35, p = 0.031). Total antioxidant, polyphenol and flavonoid
contents and quercetin concentration of the 2% extract were 2353.4 ± 56.5 μM, 313.40 ± 6.52
mg/g, 76.66 ± 23.24 mg/g, and 19.41 ± 4.34 mg/l, respectively. Calendula extract gel could be
effective on decreasing the intensity of radiotherapy- induced OM during the treatment and
antioxidant capacity may be partly responsible for the effect. © 2013 Babaee et al.; licensee
BioMed Central Ltd.
KW - Antioxidant capacity
KW - Calendula officinalis
KW - Gel formulation
KW - OMAS (oral mucositis assessment scale)
KW - Radiotherapy-induced oral mucositis
KW - antioxidant
KW - calendula officinalis extract
KW - cisplatin
KW - flavonoid
KW - fluorouracil
KW - mouthwash
KW - placebo
KW - plant extract
KW - polyphenol derivative
KW - quercetin
KW - unclassified drug
KW - adult
KW - aged
KW - antioxidant activity
KW - article
KW - cancer combination chemotherapy
KW - cancer diagnosis
KW - cancer radiotherapy
KW - chemoradiotherapy
KW - clinical article
KW - cobalt teletherapy
KW - controlled study
KW - double blind procedure
KW - drug mechanism
KW - female
KW - flower
KW - head and neck squamous cell carcinoma
KW - human
KW - male
KW - medicinal plant
KW - mucosa inflammation
KW - oropharyngeal mucositis
KW - pharynx disease
KW - radiation dose fractionation
KW - randomized controlled trial
SN - 20082231 (ISSN)
LA - English
J2 - DARU J. Pharm. Sci.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 78; Correspondence Address: D. Moslemi;
Division of Radiation Oncology, School of Medicine, Babol University of Medical Sciences,
Babol, Iran; email: moslemi_d@yahoo.com; CODEN: DJTSF
ER -

TY - JOUR
AU - Pelin, I.M.
AU - Silion, M.
AU - Popescu, I.
AU - Rîmbu, C.M.
AU - Fundueanu, G.
AU - Constantin, M.
TI - Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and
In Vitro Evaluation for Wound Healing Applications
PY - 2023
T2 - Pharmaceutics
VL - 15
IS - 6
C7 - 1674
DO - 10.3390/pharmaceutics15061674
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163844669&doi=10.3390%2fpharmace
utics15061674&partnerID=40&md5=0b49882ff7d3fb406d6fd5aff18d7a97
AD - “Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley 41A, Iasi,
700487, Romania
AD - Faculty of Veterinary Medicine “Ion Ionescu de la Brad”, University of Life Science, 8
Mihail Sadoveanu Alley, Iasi, 707027, Romania
AB - The therapeutic efficiency of plant extracts has been limited by their poor pharmaceutical
availability. Hydrogels have promising potential to be applied as wound dressings due to their
high capacity to absorb exudates and their enhanced performance in loading and releasing
plant extracts. In this work, pullulan/poly (vinyl alcohol) (P/PVA) hydrogels were first prepared
using an eco-friendly method based on both a covalent and physical cross-linking approach.
Then, the hydrogels were loaded with the hydroalcoholic extract of Calendula officinalis by a
simple post-loading immersion method. Different loading capacities were investigated in terms
of the physico-chemical properties, chemical composition, mechanical properties, and water
absorption. The hydrogels exhibited high loading efficiency due to the hydrogen bonding
interactions between polymer and extract. The water retention capacity as well as the
mechanical properties decreased with the increase in the extract amount in hydrogel. However,
higher amounts of extract in the hydrogel improved the bioadhesiveness. The release of extract
from hydrogels was controlled by the Fickian diffusion mechanism. Extract-loaded hydrogels
expressed high antioxidant activity, reaching 70% DPPH radical scavenging after 15 min
immersion in buffer solution at pH 5.5. Additionally, loaded hydrogels showed a high
antibacterial activity against Gram-positive and Gram-negative bacteria and were non-cytotoxic
against HDFa cells. © 2023 by the authors.
KW - antibacterial
KW - antioxidant
KW - Calendula officinalis extract
KW - drug delivery system
KW - pullulan/poly(vinyl alcohol) hydrogels
KW - 1,1 diphenyl 2 picrylhydrazyl
KW - alcohol
KW - antiinfective agent
KW - antioxidant
KW - buffer
KW - caffeic acid
KW - caffeic acid 3 glucoside
KW - Calendula extract
KW - Calendula officinalis extract
KW - chlorogenic acid
KW - dermatological agent
KW - dicaffeoylquinic acid
KW - flavonoid
KW - hydrogel
KW - hydroxycaffeic acid
KW - hyperin
KW - isorhamnetin
KW - isorhamnetin 3 O glucoside
KW - isorhamnetin 3 O rutinoside
KW - isorhamnetin 3,7 di O beta dextro glucopyranoside
KW - isorhamnetin rutinoside
KW - oleanolic acid glucuronide A
KW - oleanolic acid glucuronide C
KW - oleanolic acid glucuronide D
KW - phenol derivative
KW - polymer
KW - polyvinyl alcohol
KW - pullulan
KW - quercetin derivative
KW - quercitin 3 O rhamnosylrutinoside
KW - quercitin 3 O rutinoside
KW - triterpene derivative
KW - typhaneoside
KW - unclassified drug
KW - water
KW - wound healing promoting agent
KW - adhesion
KW - antibacterial activity
KW - antioxidant activity
KW - Article
KW - Calendula officinalis
KW - chemical composition
KW - controlled study
KW - covalent bond
KW - cross linking
KW - diffusion
KW - DPPH radical scavenging assay
KW - drug delivery system
KW - drug design
KW - drug release
KW - drug screening
KW - Fickian diffusion
KW - Gram negative bacterium
KW - Gram positive bacterium
KW - human
KW - human cell
KW - hydrogen bond
KW - in vitro study
KW - mucoadhesion
KW - pH
KW - physical chemistry
KW - skin fibroblast
KW - synthesis
KW - water absorption
KW - water retention
KW - wound healing
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SN - 19994923 (ISSN)
LA - English
J2 - Pharmaceutics
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: M. Constantin;
“Petru Poni” Institute of Macromolecular Chemistry, Iasi, Gr. Ghica Voda Alley 41A, 700487,
Romania; email: marieta@icmpp.ro
ER -

TY - JOUR
AU - Palacios, A.
AU - Barberón, J.
AU - Leaden, P.
AU - Zeinsteger, P.
TI - Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated
peroxidation of rat brain microsomes
PY - 2016
T2 - ADMET and DMPK
VL - 4
IS - 2
SP - 91
EP - 97
DO - 10.5599/admet.4.2.251
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047053230&doi=10.5599%2fadmet.4.2
.251&partnerID=40&md5=6c9e3e1d126978c1306e336329d6b704
AD - Cátedra Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata,
La Plata, Buenos Aires, CC296 (1900), Argentina
AB - In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the
polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of
microsomes isolated from brain rat, are presented. After incubation of microsomes in an
ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total
cpm/mg protein originated from light emission:chemiluminescence was lower in brain
microsomes obtained from CO group compared to the control group (without extract
supplementation). Moreover, it was observed that the addition of the extract reduced
chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty
acid composition of brain microsomes from control group was profoundly modified when
subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid
C20:4ω6 and docosahexaenoic acid C22:6ω3. As a consequence, the unsaturation index, a
parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO
group compared to controls. The simultaneous analysis of unsaturation index,
chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant
protecting rat brain microsomes from peroxidative damage. © 2016 International Association of
Physical Chemists.
KW - Chemiluminescence
KW - Lipoperoxidation
KW - Marigold
PB - International Association of Physical Chemists
SN - 18487718 (ISSN)
LA - English
J2 - ADMET DMPK
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: P. Zeinsteger;
Cátedra Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La
Plata, Buenos Aires, CC296 (1900), Argentina; email: pzeins@fcv.unlp.edu.ar
ER -

TY - JOUR
AU - Nicolaus, C.
AU - Junghanns, S.
AU - Hartmann, A.
AU - Murillo, R.
AU - Ganzera, M.
AU - Merfort, I.
TI - In vitro studies to evaluate the wound healing properties of Calendula officinalis extracts
PY - 2017
T2 - Journal of Ethnopharmacology
VL - 196
SP - 94
EP - 103
DO - 10.1016/j.jep.2016.12.006
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006919572&doi=10.1016%2fj.jep.2016
.12.006&partnerID=40&md5=44af03b9995fab1dcd892c7a4a03307e
AD - Institute of Pharmaceutical Sciences, Department of Pharmaceutical Biology and
Biotechnology, University of Freiburg, Stefan-Meier-Strasse 19, Freiburg, D-79104, Germany
AD - Institute of Pharmacy Pharmacognosy, University of Innsbruck, Innrain 80–82/IV,
Innsbruck, A-6020, Austria
AD - Escuela de Quimica and CIPRONA, Universidad de Costa Rica, San Jose, 2060, Costa
Rica
AB - Ethnopharmacological relevance Calendula officinalis (pot marigold) flower extracts have
a long-lasting tradition in ethnopharmacology. Currently, the European Medicines Agency (EMA)
has approved its lipophilic and aqueous alcoholic extracts as traditional medicinal products for
the treatment of minor inflammation of the skin and as an aid in the healing of minor wounds.
Aim of the study The purpose of this study was to analyse the molecular mechanism of the
wound healing effects of Calendula extracts, which may reflect the phytomedicines currently
used in the market. Materials and methods The effect of three different extracts from Calendula
flowers (n-hexanic, ethanolic, aqueous) on the inflammatory phase of wound healing was
studied in human immortalized keratinocytes and human dermal fibroblasts. An electrophoretic
mobility shift assay on NF-κB-DNA binding, qRT-PCR and ELISA experiments were performed.
The effect of Calendula extracts on the new tissue formation phase of wound healing was
evaluated by studying the migratory properties of these extracts, triterpene mixtures and single
compounds in human immortalized keratinocytes using the scratch assay. Finally, the effect of
the extracts on the formation of granulation tissue in wound healing was studied using bacterial
collagenase isolated from Clostridium histolyticum and the determination of soluble collagen in
the supernatant of human dermal fibroblasts. Results The n-hexanic and the ethanolic extracts
from Calendula flowers influence the inflammatory phase by activating the transcription factor
NF-κB and by increasing the amount of the chemokine IL-8, both at the transcriptional and
protein level, in human immortalized keratinocytes. The migration of the keratinocytes during the
new tissue formation phase was only marginally influenced in the scratch assay. However, it can
be assumed that the granulation tissue was affected, as the ethanolic extract inhibited the
activity of collagenase in vitro and enhanced the amount of collagen in the supernatant of
human dermal fibroblasts. Conclusions Our results contribute to a better understanding of the
wound healing properties of the traditional medicinal plant Calendula officinalis. However, further
studies are necessary to evaluate which of its known constituents are responsible for these
effects. Triterpenes seem to play only a marginal role, but carotene and xanthophyll derivatives
should garner more attention in future studies. © 2016 Elsevier Ireland Ltd
KW - Calendula officinalis
KW - Collagen
KW - Migration
KW - NF-κB
KW - Wound healing
KW - Calendula
KW - Cell Movement
KW - Cells, Cultured
KW - Collagen
KW - Collagenases
KW - Ethanol
KW - Fibroblasts
KW - Flowers
KW - Foreskin
KW - Hexanes
KW - Humans
KW - Interleukin-8
KW - Keratinocytes
KW - Male
KW - Plant Extracts
KW - Solvents
KW - Wound Healing
KW - alcohol
KW - aspartame
KW - bacterial enzyme
KW - Calendula extract
KW - Calendula officinalis extract
KW - clostridiopeptidase A
KW - collagen
KW - DNA
KW - flower extract
KW - hexane
KW - immunoglobulin enhancer binding protein
KW - interleukin 8
KW - unclassified drug
KW - wound healing promoting agent
KW - collagen
KW - collagenase
KW - IL8 protein, human
KW - interleukin 8
KW - n-hexane
KW - plant extract
KW - solvent
KW - Article
KW - Calendula officinalis
KW - cell viability
KW - Clostridium histolyticum
KW - controlled study
KW - drug mechanism
KW - drug screening
KW - enzyme isolation
KW - flower head
KW - gel mobility shift assay
KW - human
KW - human cell
KW - immortalized cell line
KW - in vitro study
KW - keratinocyte
KW - male
KW - nonhuman
KW - normal human
KW - quantitative analysis
KW - real time polymerase chain reaction
KW - reverse transcription polymerase chain reaction
KW - skin fibroblast
KW - wound healing
KW - Calendula
KW - cell culture
KW - cell motion
KW - chemistry
KW - cytology
KW - drug effects
KW - fibroblast
KW - flower
KW - genetics
KW - metabolism
KW - physiology
KW - prepuce
KW - wound healing
PB - Elsevier Ireland Ltd
SN - 03788741 (ISSN)
C2 - 27956358
LA - English
J2 - J. Ethnopharmacol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 87; Correspondence Address: I. Merfort;
Freiburg, Stefan-Meier-Strasse 19, Germany; email:
irmgard.merfort@pharmazie.uni-freiburg.de; CODEN: JOETD
ER -

TY - JOUR
AU - Alnuqaydan, A.M.
AU - Lenehan, C.E.
AU - Hughes, R.R.
AU - Sanderson, B.J.
TI - Extracts from Calendula officinalis offer in vitro protection against H2O2 induced oxidative
stress cell killing of human skin cells
PY - 2015
T2 - Phytotherapy Research
VL - 29
IS - 1
SP - 120
EP - 124
DO - 10.1002/ptr.5236
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920936343&doi=10.1002%2fptr.5236&
partnerID=40&md5=b08463089d465e8912189508f51d013b
AD - School of Medicine, Faculty of Medicine, Nursing and Health Sciences, Flinders
University, Australia
AD - School of Chemical and Physical Sciences, Faculty of Science and Engineering, Flinders
University, Australia
AB - The in vitro safety and antioxidant potential of Calendula officinalis flower head extracts
was investigated. The effect of different concentrations (0.125, 0.5, 1.0, 2.0 and 5.0% (v/v)) of
Calendula extracts on human skin cells HaCaT in vitro was explored. Doses of 1.0% (v/v)
(0.88mg dry weight/mL) or less showed no toxicity. Cells were also exposed to the Calendula
extracts for either 4, 24 or 48 h before being exposed to an oxidative insult (hydrogen peroxide
H2O2) for 1 h. Using the MTT cytotoxicity assay, it was observed that two independent extracts
of C. officinalis gave time-dependent and concentration-dependent H2O2 protection against
induced oxidative stress in vitro using human skin cells. Pre-incubation with the Calendula
extracts for 24 and 48 h increased survival relative to the population without extract by 20% and
40% respectively following oxidative challenge. The antioxidant potential of the Calendula
extracts was confirmed using a complimentary chemical technique, the DPPH• assay. Calendula
extracts exhibited free radical scavenging abilities. This study demonstrates that Calendula
flower extracts contain bioactive and free radical scavenging compounds that significantly
protect against oxidative stress in a human skin cell culture model. Copyright © 2014 John
Wiley & Sons, Ltd.
KW - Antioxidants
KW - Calendula officinalis
KW - Cytotoxicity
KW - Flower extracts
KW - Human cells.
KW - Oxidative stress
KW - Antioxidants
KW - Calendula
KW - Cell Line
KW - Flowers
KW - Humans
KW - Hydrogen Peroxide
KW - Oxidative Stress
KW - Plant Extracts
KW - Skin
KW - Calendula
KW - Calendula officinalis
KW - 1,1 diphenyl 2 picrylhydrazyl
KW - 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
KW - antioxidant
KW - Calendula officinalis extract
KW - plant extract
KW - unclassified drug
KW - antioxidant
KW - hydrogen peroxide
KW - plant extract
KW - antioxidant activity
KW - Article
KW - Calendula officinalis
KW - cell killing
KW - cell population
KW - cell protection
KW - cell survival
KW - cell viability
KW - concentration response
KW - controlled study
KW - cytotoxicity assay
KW - DPPH radical scavenging assay
KW - drug effect
KW - drug safety
KW - human
KW - human cell
KW - in vitro study
KW - oxidative stress
KW - skin cell
KW - Calendula
KW - cell line
KW - chemistry
KW - cytology
KW - drug effects
KW - flower
KW - oxidative stress
KW - skin
PB - John Wiley and Sons Ltd
SN - 0951418X (ISSN)
C2 - 25266574
LA - English
J2 - Phytother. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 20; CODEN: PHYRE
ER -
TY - JOUR
AU - Besil, N.
AU - Pequeño, F.
AU - Alonzo, N.
AU - Hladki, R.
AU - Cesio, M.V.
AU - Heinzen, H.
TI - Evaluation of different QuEChERS procedures for pesticide residues determination in
Calendula officinalis (L) inflorescences
PY - 2017
T2 - Journal of Applied Research on Medicinal and Aromatic Plants
VL - 7
SP - 143
EP - 148
DO - 10.1016/j.jarmap.2017.09.001
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030791800&doi=10.1016%2fj.jarmap.2
017.09.001&partnerID=40&md5=8377b237451ebf54e835bcceae467cd5
AD - Grupo de Análisis de Contaminantes Trazas (GACT), Departamento de Química del
Litoral, Facultad de Química, CENUR Litoral Norte, Universidad de la República, Ruta 3, Km
363, Paysandú, 60000, Uruguay
AD - Grupo de Análisis de Contaminantes Traza, Cátedra de Farmacognosia y Productos
Naturales, Facultad de Química, Universidad de la República, General Flores 2124,
Montevideo, 11800, Uruguay
AD - Polo de Desarrollo Universitario: “Abordaje holístico a una temática tan innovadora como
preocupante: impacto de los agroquímicos sobre los productos agroalimentarios y el ambiente”,
CENUR Litoral Norte, Universidad de la República, Ruta 3, Km 363, Paysandú, 60000, Uruguay
AB - Calendula officinalis L. (marigold) is a medicinal plant consumed at ton scale worldwide.
For that reason the raw material supply cannot rely solely on the collection of wild specimens. C.
officinalis is cultivated all over the world not only to cover the increasing demand but also to
ensure its regular quality. When conventional cropping systems are employed, the use of
pesticides to protect the cultures against pests is a common practice. The presence of
contaminants in herbs for medicinal purposes is strictly ruled by Pharmacopoeias. The
European pharmacopoeia, as well as the MERCOSUR, require validated methods to determine
the content of pesticide residues in each specific medicinal plant. As a contribution to
accomplish such requirements, in the present work a comparison of the performance of three
modern multirresidue methods based on QuEChERS methodology was conducted. Aiming to
find a method fit for that purpose, the original, unbuffered version and variationss of the AOAC
2007.01 and the EN 15662 protocols were evaluated in marigold for the determination of 24
pesticide residues using LC–MS/MS. The three evaluated methods where accurate and precise
for all the studied compounds but the original QuEChERS extracts causes less matrix effects
over the studied pesticides. After the evaluation of the figures of merits, the selected
methodology was validated according to the SANTE guidelines. LOQs were determined in the
range 10–100 μg kg−1 showing a linear behaviour (r2 > 0.993) from LOQ to 400 μg kg−1. The
method was applied in commercial samples where atrazine, imidacloprid, pirimiphos-methyl and
methalaxyl residues were detected. © 2017 Elsevier GmbH
KW - Calendula officinalis
KW - LC–MS/MS
KW - Medicinal herbs
KW - Pesticides
KW - abamectin
KW - acetamiprid
KW - atrazine
KW - azoxystrobin
KW - buprofezin
KW - Calendula extract
KW - carbaril
KW - carbendazim
KW - clomazone
KW - enilconazole
KW - imidacloprid
KW - iprodione
KW - metalaxyl
KW - methamidophos
KW - methiocarb
KW - methomyl
KW - pesticide residue
KW - pirimicarb
KW - pirimifosmethyl
KW - pirimiphos methyl
KW - propanil
KW - propiconazole
KW - pyrimethanil
KW - skin cream
KW - spinosad
KW - spinosyn A
KW - tebuconazole
KW - thiamethoxam
KW - unclassified drug
KW - analytical parameters
KW - Article
KW - Calendula officinalis
KW - inflorescence
KW - limit of detection
KW - limit of quantitation
KW - linearity
KW - liquid chromatography-mass spectrometry
KW - measurement precision
KW - measurement repeatability
KW - medical literature
KW - medicinal plant
KW - multiple reaction monitoring
KW - nonhuman
KW - priority journal
KW - reproducibility
KW - sensitivity and specificity
KW - trueness
KW - validation process
PB - Elsevier GmbH
SN - 22147861 (ISSN)
LA - English
J2 - J. Appl. Res. Med. Aromat. Plants
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 12; Correspondence Address: H. Heinzen;
Cátedra de Farmacognosia y Productos Naturales, Departamento de Química Orgánica,
Facultad de Química, Universidad de la República. General Flores 2124, Montevideo, 11800,
Uruguay; email: heinzen@fq.edu.uy
ER -

TY - JOUR
AU - Shurigin, V.
AU - Alaylar, B.
AU - Davranov, K.
AU - Wirth, S.
AU - Bellingrath-Kimura, S.D.
AU - Egamberdieva, D.
TI - Diversity and biological activity of culturable endophytic bacteria associated with marigold
(Calendula officinalis l.)
PY - 2021
T2 - AIMS Microbiology
VL - 7
IS - 3
SP - 336
EP - 353
DO - 10.3934/microbiol.2021021
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116337345&doi=10.3934%2fmicrobiol.
2021021&partnerID=40&md5=3504d3a4915d1211028342c2c54750d5
AD - Department of Microbiology and Biotechnology, Faculty of Biology, National University of
Uzbekistan, Tashkent, 100174, Uzbekistan
AD - Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Agri Ibrahim
Cecen University, Agri, 04100, Turkey
AD - Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan,
Tashkent, 100128, Uzbekistan
AD - Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, 15374,
Germany
AD - Faculty of Life Science, Humboldt University of Berlin, Berlin, 14195, Germany
AB - Endophytes colonizing plant tissue play an essential role in plant growth, development,
stress tolerance and plant protection from soil-borne diseases. In this study, we report the
diversity of cultivable endophytic bacteria associated with marigold (Calendula officinalis L.) by
using 16S rRNA gene analysis and their plant beneficial properties. A total of 42 bacterial
isolates were obtained from plant tissues of marigold. They belonged to the genera Pantoea,
Enterobacter, Pseudomonas, Achromobacter, Xanthomonas, Rathayibacter, Agrobacterium,
Pseudoxanthomonas, and Beijerinckia. Among the bacterial strains, P. kilonensis FRT12, and P.
rhizosphaerae FST5 showed moderate or vigorous inhibition against three tested plant
pathogenic fungi, F. culmorum, F. solani and R. solani. They also demonstrated the capability to
produce hydrolytic enzymes and indole-3-acetic acid (IAA). Five out of 16 isolates significantly
stimulated shoot and root growth of marigold in a pot experiment. The present study reveals that
more than half of the bacterial isolates associated with marigold (C. officinalis L.) provided
antifungal activity against one or more plant pathogenic fungi. Our findings suggest that
medicinal plants with antimicrobial activity could be a source for selecting microbes with
antagonistic activity against fungal plant pathogens or with plant growth stimulating potential.
These isolates might be considered as promising candidates for the improvement of plant
health. © 2021 the Author(s), licensee AIMS Press.
KW - Antagonism
KW - Auxin
KW - Endophyte
KW - Marigold
KW - Plant growth promotion
PB - AIMS Press
SN - 24711888 (ISSN)
LA - English
J2 - AIMS Microb.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 12; Correspondence Address: D. Egamberdieva;
Department of Microbiology and Biotechnology, Faculty of Biology, National University of
Uzbekistan, Tashkent, 100174, Uzbekistan; email: Dilfuza.Egamberdieva@zalf.de
ER -

TY - JOUR
AU - Shahane, K.
AU - Kshirsagar, M.
AU - Tambe, S.
AU - Jain, D.
AU - Rout, S.
AU - Ferreira, M.K.M.
AU - Mali, S.
AU - Amin, P.
AU - Srivastav, P.P.
AU - Cruz, J.
AU - Lima, R.R.
TI - An Updated Review on the Multifaceted Therapeutic Potential of Calendula officinalis L.
PY - 2023
T2 - Pharmaceuticals
VL - 16
IS - 4
C7 - 611
DO - 10.3390/ph16040611
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85154576106&doi=10.3390%2fph160406
11&partnerID=40&md5=c1d826c2907ed362df6ed53b9cc68a54
AD - Department of Pharmaceutical Sciences and Technology, Institute of Chemical
Technology, Mumbai, 400019, India
AD - Department of Agricultural and Food Engineering, Indian Institute of Technology,
Kharagpur, 721302, India
AD - Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal
University of Pará, Belém, 66075-110, Brazil
AD - Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology,
Mesra, 835215, India
AB - Calendula officinalis Linn. (CO) is a popular medicinal plant from the plant kingdom’s
Asteraceae family that has been used for millennia. This plant contains flavonoids, triterpenoids,
glycosides, saponins, carotenoids, volatile oil, amino acids, steroids, sterols, and quinines.
These chemical constituents confer multifaceted biological effects such as anti-inflammatory,
anti-cancer, antihelminthic, antidiabetes, wound healing, hepatoprotective, and antioxidant
activities. Additionally, it is employed in cases of certain burns and gastrointestinal,
gynecological, ocular, and skin conditions. In this review, we have discussed recent research
from the last five years on the therapeutic applications of CO and emphasized its myriad
capabilities as a traditional medicine. We have also elucidated CO’s molecular mechanisms and
recent clinical studies. Overall, this review intends to summarize, fill in the gaps in the existing
research, and provide a wealth of possibilities for researchers working to validate traditional
claims and advance the safe and effective use of CO in treating various ailments. © 2023 by the
authors.
KW - biological activities
KW - Calendula officinalis
KW - chemical composition
KW - traditional medicine
KW - amino acid
KW - antidiabetic agent
KW - carotenoid
KW - coumarin
KW - essential oil
KW - flavonoid
KW - glycoside
KW - phenol derivative
KW - quinine
KW - quinone derivative
KW - saponin
KW - steroid
KW - sterol
KW - terpenoid
KW - triterpenoid
KW - anthelmintic activity
KW - antibacterial activity
KW - antidiabetic activity
KW - antifungal activity
KW - antiinflammatory activity
KW - antimicrobial activity
KW - antineoplastic activity
KW - antioxidant activity
KW - antiprotozoal activity
KW - Asteraceae
KW - biological activity
KW - Calendula officinalis
KW - chemical composition
KW - cytotoxicity
KW - human
KW - liver protection
KW - nonhuman
KW - Review
KW - therapy effect
KW - traditional medicine
KW - wound healing
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SN - 14248247 (ISSN)
LA - English
J2 - Pharmaceuticals
M3 - Review
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 6; Correspondence Address: R.R. Lima;
Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal
University of Pará, Belém, 66075-110, Brazil; email: rafalima@ufpa.br
ER -

TY - JOUR
AU - Tsalgatidou, P.C.
AU - Thomloudi, E.-E.
AU - Nifakos, K.
AU - Delis, C.
AU - Venieraki, A.
AU - Katinakis, P.
TI - Calendula officinalis—A Great Source of Plant Growth Promoting Endophytic Bacteria
(PGPEB) and Biological Control Agents (BCA)
PY - 2023
T2 - Microorganisms
VL - 11
IS - 1
C7 - 206
DO - 10.3390/microorganisms11010206
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85146802953&doi=10.3390%2fmicroorga
nisms11010206&partnerID=40&md5=5677706a4a8f0ca6fad6f4808141ac1f
AD - Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera
Odos 75, Athens, 11855, Greece
AD - Department of Agriculture, University of the Peloponnese, Kalamata, 24100, Greece
AD - Laboratory of Plant Pathology, Agricultural University of Athens, Iera Odos 75, Athens,
11855, Greece
AB - The application of beneficial bacteria may present an alternative approach to chemical
plant protection and fertilization products as they enhance growth and resistance to biotic and
abiotic stresses. Plant growth-promoting bacteria are found in the rhizosphere, epiphytically or
endophytically (Plant Growth Promoting Endophytic Bacteria, PGPEB). In the present study, 36
out of 119 isolated endophytic bacterial strains from roots, leaves and flowers of the
pharmaceutical plant Calendula officinalis were further identified and classified into Bacillus,
Pseudomonas, Pantoea, Stenotrophomonas and Rhizobium genera. Selected endophytes were
evaluated depending on positive reaction to different plant growth promoting (PGP) traits,
motility, survival rate and inhibition of phytopathogenic fungi in vitro and ex vivo (tomato fruit).
Bacteria were further assessed for their plant growth effect on Arabidopsis thaliana seedlings
and on seed bio-primed tomato plantlets, in vitro. Our results indicated that many bacterial
endophytes increased seed germination, promoted plant growth and changed root structure by
increasing lateral root density and length and root hair formation. The most promising
antagonistic PGPEB strains (Cal.r.29, Cal.l.30, Cal.f.4, Cal.l.11, Cal.f.2.1, Cal.r.19 and Cal.r.11)
are indicated as effective biological control agents (BCA) against Botrytis cinerea on detached
tomato fruits. Results underlie the utility of beneficial endophytic bacteria for sustainable and
efficient crop production and disease control. © 2023 by the authors.
KW - biological control
KW - Botrytis cinerea
KW - Calendula officinalis
KW - detached fruit assay
KW - plant growth promoting endophytic bacteria
PB - MDPI
SN - 20762607 (ISSN)
LA - English
J2 - Microorg.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 5; Correspondence Address: P.C. Tsalgatidou;
Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Athens,
Iera Odos 75, 11855, Greece; email: polina.tsalgatidou@go.uop.gr; A. Venieraki; Laboratory of
Plant Pathology, Agricultural University of Athens, Athens, Iera Odos 75, 11855, Greece; email:
venieraki@aua.gr
ER -

TY - JOUR
AU - Jiménez, R.A.
AU - Millán, D.
AU - Suesca, E.
AU - Sosnik, A.
AU - Fontanilla, M.R.
TI - Controlled release of an extract of Calendula officinalis flowers from a system based on the
incorporation of gelatin-collagen microparticles into collagen I scaffolds: design and in vitro
performance
PY - 2015
T2 - Drug Delivery and Translational Research
VL - 5
IS - 3
SP - 209
EP - 218
DO - 10.1007/s13346-015-0217-3
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928885845&doi=10.1007%2fs13346-0
15-0217-3&partnerID=40&md5=09b68c59ff880ab3e872e6b6ba752c59
AD - Tissue Engineering Group, Department of Pharmacy, Universidad Nacional de Colombia,
Av. Carrera 30 # 45-10, Bogotá, Colombia
AD - Universidad El Bosque, Bogotá, Colombia
AD - Department of Materials Science and Engineering, Technion-Israel Institute of Technology,
Technion City, Haifa, Israel
AB - [Figure not available: see fulltext.]; Aiming to develop biological skin dresses with
improved performance in the treatment of skin wounds, acellular collagen I scaffolds were
modified with polymeric microparticles and the subsequent loading of a hydroglycolic extract of
Calendula officinalis flowers. Microparticles made of gelatin-collagen were produced by a
water-in-oil emulsion/cross-linking method. Thereafter, these microparticles were mixed with
collagen suspensions at three increasing concentrations and the resulting mixtures lyophilized
to make microparticle-loaded porous collagen scaffolds. Resistance to enzymatic degradation,
ability to associate with the C. officinalis extract, and the extract release profile of the three
gelatin-collagen microparticle-scaffold prototypes were assessed in vitro and compared to
collagen scaffolds without microparticles used as control. Data indicated that the incorporation
of gelatin-collagen microparticles increased the resistance of the scaffolds to in vitro enzymatic
degradation, as well as their association with the C. officinalis flower extract. In addition, a sharp
decrease in cytotoxicity, as well as more prolonged release of the extract, was attained. Overall
results support the potential of these systems to develop innovative dermal substitutes with
improved features. Furthermore, the gelatin-collagen mixture represents a low-cost and scalable
alternative with high clinical transferability, especially appealing in developing countries. © 2015,
Controlled Release Society.
KW - Calendula officinalis L. flowers extract
KW - Collagen I scaffolds
KW - Gelatin-collagen microparticle-loaded skin dressings
KW - Polyphenols
KW - Wound healing
KW - Administration, Cutaneous
KW - Animals
KW - Anti-Inflammatory Agents, Non-Steroidal
KW - Antioxidants
KW - Calendula
KW - Cell Line
KW - Cell Survival
KW - Collagen Type I
KW - Delayed-Action Preparations
KW - Dermatologic Agents
KW - Drug Carriers
KW - Drug Compounding
KW - Drug Stability
KW - Flowers
KW - Freeze Drying
KW - Gelatin
KW - Mice
KW - Microspheres
KW - Plant Extracts
KW - Skin
KW - Solubility
KW - Wound Healing
KW - Calendula officinalis extract
KW - collagen type 1
KW - flower extract
KW - gelatin
KW - polyphenol
KW - unclassified drug
KW - water oil cream
KW - antioxidant
KW - collagen type 1
KW - delayed release formulation
KW - dermatological agent
KW - drug carrier
KW - gelatin
KW - microsphere
KW - nonsteroid antiinflammatory agent
KW - plant extract
KW - antioxidant activity
KW - Article
KW - controlled drug release
KW - cross linking
KW - enzymatic degradation
KW - flower
KW - in vitro study
KW - in vivo study
KW - molecular interaction
KW - nonhuman
KW - priority journal
KW - skin injury
KW - tissue engineering
KW - administration and dosage
KW - adverse effects
KW - animal
KW - Calendula
KW - cell line
KW - cell survival
KW - chemistry
KW - comparative study
KW - delayed release formulation
KW - drug effects
KW - drug formulation
KW - drug stability
KW - freeze drying
KW - injuries
KW - intradermal drug administration
KW - mouse
KW - skin
KW - solubility
KW - wound healing
PB - Springer Verlag
SN - 2190393X (ISSN)
C2 - 25787728
LA - English
J2 - Drug Deliv. Transl. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 23
ER -

TY - JOUR
AU - Colombo, E.
AU - Sangiovanni, E.
AU - D'Ambrosio, M.
AU - Bosisio, E.
AU - Ciocarlan, A.
AU - Fumagalli, M.
AU - Guerriero, A.
AU - Harghel, P.
AU - Dell'Agli, M.
TI - A Bio-Guided Fractionation to Assess the Inhibitory Activity of Calendula officinalis L. on the
NF- B Driven Transcription in Human Gastric Epithelial Cells
PY - 2015
T2 - Evidence-based Complementary and Alternative Medicine
VL - 2015
C7 - 727342
DO - 10.1155/2015/727342
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84944243275&doi=10.1155%2f2015%2f7
27342&partnerID=40&md5=ac8ebcb3ca6f660f2037148a257be300
AD - Department of Pharmacological and Biomolecular Sciences, Università Degli Studi di
Milano, Via Balzaretti 9, Milan, 20133, Italy
AD - Laboratory of Bioorganic Chemistry, Department of Physics, Università Degli Studi di
Trento, Via Sommarive 14, Trento, 38123, Italy
AB - Calendula officinalis L. has been largely known for its topical anti-inflammatory properties;
however, there are no experimental evidences about its antiphlogistic effect at the gastric level.
To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2
extract obtained from C. officinalis flowers was evaluated in vitro on the NF-B pathway. The
lipophilic extract demonstrated a significant inhibitory effect on the NF-B driven transcription.
The identification of active compounds was conducted by a bio-guided fractionation of the
extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory
activity on the NF-B driven transcription and significantly contributed to the antiphlogistic effect
showed by CH2Cl2 extract. The main components of fraction J were loliolide and the fucoside
acetates of β-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the
identification and isolation of triterpene esters that showed a concentration-dependent inhibition
of the NF-B driven transcription, with faradiol-3-myristate and the corresponding aglycone being
the most active compounds. The present study provides some experimental evidences that
Calendula officinalis L. may exert an anti-inflammatory activity on the gastric district by the
inhibition of the NF-B system, identifying the compounds responsible, at least in part, for the
observed effect. © 2015 Elisa Colombo et al.
KW - acetic acid derivative
KW - aglycone
KW - antiinflammatory agent
KW - beta eudesmol
KW - Calendula officinalis extract
KW - faradiol 3 myristate
KW - fucoside acetate
KW - immunoglobulin enhancer binding protein
KW - loliolide
KW - myristic acid
KW - plant extract
KW - triterpene
KW - triterpene ester
KW - unclassified drug
KW - viridiflorol
KW - antiinflammatory activity
KW - Article
KW - cell fractionation
KW - concentration response
KW - controlled study
KW - drug identification
KW - drug isolation
KW - epithelium cell
KW - flower
KW - genetic transcription
KW - high performance liquid chromatography
KW - human
KW - human cell
KW - IC50
KW - in vitro study
KW - lipophilicity
KW - priority journal
KW - stomach epithelium
PB - Hindawi Publishing Corporation
SN - 1741427X (ISSN)
LA - English
J2 - Evid.-Based Complement. Altern. Med.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 7
ER -

TY - JOUR
AU - Ghédira, K.
AU - Goetz, P.
TI - Calendula officinalis L. (Asteraceae): Marigold
ST - Calendula officinalis L. (Asteraceae) : souci
PY - 2016
T2 - Phytotherapie
VL - 14
IS - 1
SP - 62
EP - 67
DO - 10.1007/s10298-016-1022-y
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958092601&doi=10.1007%2fs10298-0
16-1022-y&partnerID=40&md5=6b05b115581f9e1206b07637a9163e76
AD - Laboratoire de pharmacognosie, rue Avicenne, Monastir, 5000, Tunisia
AD - Dumenat de phytothérapie, Paris-XIII, Bobigny cedex, F-93017, France
KW - Article
KW - Calendula officinalis
PB - Springer-Verlag France
SN - 16248597 (ISSN)
LA - English
J2 - Phytotherapie
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2
ER -
TY - JOUR
AU - Giostri, G.S.
AU - Novak, E.M.
AU - Buzzi, M.
AU - Guarita-Souza, L.C.
TI - Treatment of acute wounds in hand with Calendula officinalis L.: A randomized trial
PY - 2022
T2 - Tissue Barriers
VL - 10
IS - 3
C7 - 1994822
DO - 10.1080/21688370.2021.1994822
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117454948&doi=10.1080%2f21688370
.2021.1994822&partnerID=40&md5=701fc5842bce09c76542fd87583eeace
AD - School of Medicine, Pontifical Catholic University of Parana (PUCPR), Hospital
Universitario Cajuru, Curitiba, Brazil
AD - Triskelion Consultoria em Pesquisa e Desenvolvimento Farmacêutico, Curitiba, Brazil
AD - School of Medicine, Pontifical Catholic University of Parana (PUCPR), Institute of
Biological and Health Sciences (CCBS), Curitiba, Brazil
AB - Most injuries in the hand and fingers, especially on the digital pulps, are suited for healing
by secondary intention. Nevertheless, delay in epithelization seems to unfavorably restrict this
technique. The purpose of this controlled randomized clinical trial is to analyze by means of
photo planimetry the progression of the healing process by secondary intention in acute wounds
of the hand using the standardized extract of Calendula officinalis L. (SEC). The cohort of
eligible participants included two groups of 20 patients with skin loss in the hand and fingers
treated by secondary intention. Control group (CG) used mineral oil and intervention group (IG)
received SEC. Wound pictures were captured at each outpatient assessment until epithelization
was achieved and measured with ImageJ. Intervention group (IG) and control group (CG) with
19 wounds each, primarily formed by men in their 40’s with wounds in their index and ring
fingers on the left side, showed homogeneous variables and similar initial wound areas.
Epithelization time was shorter and healing speed was faster in IG (IG = 8.6 ± 4.7 days and
9.5 ± 5.8%day versus CG = 13.2 ± 7.4 days and 6.2 ± 2.9%day, ƿ < 0.05), leading to the
conclusion that healing by secondary intention in acute wounds of the hand and fingers with
SEC led to a faster epithelization. © 2021 Taylor & Francis Group, LLC.
KW - Calendula officinalis
KW - finger injuries
KW - Wound healing
KW - Calendula
KW - Humans
KW - Male
KW - Plant Extracts
KW - Wound Healing
KW - plant extract
KW - adult
KW - Article
KW - Calendula officinalis
KW - clinical article
KW - controlled study
KW - disease control
KW - epithelization
KW - female
KW - granulation tissue
KW - human
KW - male
KW - necrosis
KW - randomized controlled trial
KW - sanitary surveillance
KW - skin defect
KW - smoking
KW - wound
KW - wound healing
KW - Calendula
KW - wound healing
PB - Taylor and Francis Ltd.
SN - 21688362 (ISSN)
C2 - 34674610
LA - English
J2 - Tissue Barriers
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: G.S. Giostri; School
of Medicine, Pontifical Catholic University of Parana, Hospital Universitario Cajuru, Curitiba,
Brazil; email: ggiostri@gmail.com
ER -

TY - JOUR
AU - Toropova, A.A.
AU - Badmaev, N.S.
AU - Razuvaeva, Ya.G.
AU - Nikolaev, S.M.
AU - Sambueva, Z.G.
AU - Erentueva, A.
TI - Influence of calendula officinalis extract on the antioxidant and energy status of liver in rats
with experimental hepatitis
PY - 2017
T2 - Eksperimental'naya i Klinicheskaya Farmakologiya
VL - 80
IS - 7
SP - 11
EP - 14
DO - 10.30906/0869-2092-2017-80-7-11-14
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040200850&doi=10.30906%2f0869-20
92-2017-80-7-11-14&partnerID=40&md5=8f6ba0378b1dc7ab50092f3848f1db34
AD - Institute of General and Experimental Biology, Siberian Branch, Russian Academy of
Sciences, ul. Sakhyanova 6, Ulan-Ude, Buryat Republic, 670047, Russian Federation
AD - Buryat State University, ul. Smolina 24, Ulan-Ude, Buryat Republic, 670000, Russian
Federation
AB - The hepatoprotective effect of Calendula officinalis L. extract was studied on the
experimental model of acute toxic hepatitis induced by administration of CCl4 in rats.
Administration of the C. officinalis extract in a dose of 100 mg/kg (p.o.) for 13 days decreased
the severity of oxidative stress by increasing the activity of key enzymes (catalase, SOD, GSH).
The phytopreparation increased the activity of hepatocytic enzymes as glutathione peroxidase
by 15% (δ < 0.05) and glutathione reductase by 39% in the rat liver tissue (relative to control) as
well as reduced the content of malondialdehyde by 28% (δ < 0.05). It was also found that the
extract of C. officinalis positively influenced the energy metabolism in hepatocytes as
manifested by increasing the ATP content by 28% (p < 0.05), decreasing lactate level by 31% (p
< 0.05), and normalizing lactate/pyruvate ratio in the liver tissue of rats with experimental toxic
hepatitis. The obtained results allow the proposed extract of C. officinalis to be recommended
for further investigation as a promising plant hepatoprotector.
KW - Calendula officinalis
KW - Energy status
KW - Hepatitis
KW - Hepatoprotective effect
KW - Oxidative stress
KW - Rats
KW - adenosine triphosphate
KW - antioxidant
KW - Calendula extract
KW - Calendula officinalis extract
KW - catalase
KW - glutathione
KW - glutathione peroxidase
KW - lactic acid
KW - liver protective agent
KW - pyruvic acid
KW - superoxide dismutase
KW - unclassified drug
KW - animal cell
KW - animal experiment
KW - animal model
KW - animal tissue
KW - Article
KW - energy metabolism
KW - experimental hepatitis
KW - liver cell
KW - liver tissue
KW - nonhuman
KW - oxidative stress
KW - rat
PB - Izdatel'stvo Meditsina
SN - 08692092 (ISSN)
LA - Russian
J2 - Eksp. Klinicheskaya Farmakol.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2
ER -

TY - JOUR
AU - Xing, Z.-F.
AU - Cheng, H.-D.
AU - Zhang, P.-P.
AU - Gong, L.
AU - Ma, L.-Y.
TI - HPLC fingerprint of Calendula officinalis flower
PY - 2014
T2 - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
VL - 37
IS - 7
SP - 1176
EP - 1178
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937117933&partnerID=40&md5=e90bf
51c692109f57ad278cbf7099938
AD - Pharmacy Department, Shandong Wanjie Medical College, Zibo, China
AB - OBJECTIVE: To establish an HPLC fingerprint of Calendula officinalis flower for its quality
control.; METHODS: Hypersil ODS C18 column (250 mm x 4.6 mm, 5 μm) was used with
acetonitrile and water as mobile phase in a gradient mode at the flow rate of 1.0 mL/min. The
detection wavelength was 220 nm and the temperature of column was set at 35 degrees C. The
similarity was analyzed with the Estimating System of Similarity on the Chinese Medicine
Fingerprint Chromatogram.; RESULTS: The HPLC fingerprint of Calendula officinalis flower
containing eleven peaks was set up. The similarity of Calendula officinalis flower from different
habitats was greater than 0.90.; CONCLUSION: This method is easy and reliable, which can be
used to judge the habitat and control the quality of Calendula officinalis flower.
KW - Calendula
KW - Chromatography, High Pressure Liquid
KW - Flowers
KW - Quality Control
KW - Calendula
KW - chemistry
KW - flower
KW - high performance liquid chromatography
KW - quality control
SN - 10014454 (ISSN)
C2 - 25566652
LA - Chinese
J2 - Zhong Yao Cai
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2
ER -

TY - JOUR
AU - Rafiee, H.
AU - Mehrafarin, A.
AU - Qaderi, A.
AU - Kalate Jari, S.
AU - Naghdi Badi, H.
TI - Phytochemical, agronomical and morphological responses of pot marigold (Calendula
officinalis L.) to foliar application of bio-stimulators (bioactive amino acid compounds)
PY - 2013
T2 - Journal of Medicinal Plants
VL - 12
IS - 47
SP - 48
EP - 61
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84888026621&partnerID=40&md5=349b
8bed91b2ca798624c44e2d4ad0a3
AD - Department of Horticulture, Science and Research Branch, Islamic Azad University,
Tehran, Iran
AD - Cultivation and Development Department of Medicinal Plants Research Center, Institute of
Medicinal Plants, ACECR, Karaj, Iran
AD - Biotechnology Department of Medicinal Plants Research Center, Institute of Medicinal
Plants, ACECR, Karaj, Iran
AB - Background: Bio-stimulators as biological substances stimulate metabolism and metabolic
processes, which can be increase plants yield and secondary metabolites content. Objective:
The aim is to introduce bio-stimulators as bioactive amino acid compounds to increase yield and
help to sustainable agriculture. Methods: This experiment was conducted at ACECR on the
base of randomized complete blocks design with three replications in 2011. The treatments
were commercial formulations of Aminolforte, Kadostim, Fosnutren, Humiforte (each of them
0.75 and 1.5 L. ha-1), chemical fertilizer NPK (70 kg. ha-1), and control treatment (no foliar
application). Results: The treatments had significant effect on the studied parameters except
SPAD value. Humiforte 1.5 L. ha-1 caused increase in plant dry weight to 37.46%, capitula dry
weight to 36.92%, leaf area to 34.44%, relative water content to 32.03%, harvest index to
20.40%, capitula number/plant to 40.64%, total carbohydrates of capitula to 18.43%, total
flavonoids of leaves to 19.35%, and total flavonoids yield of leaves to 38.63% compared to
control. The highest amount of total flavonoids in capitula (0.25%, with increase to 32%
compared to control) was related to Aminolforte 1.5 L.ha-1. Kadostim 1.5 L. ha-1 increased
plant height to 36.83%, leaves number to 26.65% and total flavonoids yield of capitula to
38.82%, total carbohydrates of leaves to 13.52%. Content of N, P and K increased with foliar
application of bioactive amino acid compounds. Conclusion: Humiforte and Kadostim 1.5 L.ha-1
were the best treatments in regard to existence of amino acid compounds and macro-nutrients
of N, P and K in their formulations.
KW - Bioactive amino acid compounds
KW - Calendula officinalis L.
KW - Morphological and Phytochemical traits
KW - Calendula officinalis
KW - amino acid derivative
KW - aminolforte
KW - carbohydrate
KW - fertilizer
KW - flavonoid
KW - fosnutren
KW - humiforte
KW - kadostim
KW - nitrogen
KW - potassium
KW - unclassified drug
KW - agronomy
KW - article
KW - Calendula officinalis
KW - controlled study
KW - dry weight
KW - harvest index
KW - latitude
KW - leaf area
KW - longitude
KW - macronutrient
KW - nonhuman
KW - pH
KW - phytochemistry
KW - plant height
KW - plant response
KW - plant structures
KW - soil texture
KW - sustainable agriculture
KW - water content
PB - Institute of Medicinal Plants
SN - 2717204X (ISSN)
LA - English
J2 - J. Med. Plants
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 14; Correspondence Address: H. Naghdi Badi;
Medicinal Plants Research Center, Institute of Medicinal Plants, Iranian Academic Center for
Education, Culture and Research (ACECR), Karaj, P.O.Box: 33651/66571, Iran; email:
Naghdibadi@yahoo.com
ER -

TY - JOUR
AU - Chamansara, R.
AU - Rashidfarokhi, R.
AU - Sakallı, E.A.
AU - Öztinen, N.
AU - Koşar, M.
TI - Comparison of Commercial Calendula officinalis L. Samples with Pharmacopeial Drug:
Antiradical Activities and Chemical Profiles
PY - 2022
T2 - Journal of Research in Pharmacy
VL - 26
IS - 4
SP - 809
EP - 819
DO - 10.29228/jrp.178
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133895061&doi=10.29228%2fjrp.178&
partnerID=40&md5=2c14a5c05db5015c7893f04600c56b13
AD - Eastern Mediterranean University, Faculty of Pharmacy, North Cyprus via Mersin-10,
Famagusta, Turkey
AB - Calendula officinalis L. (Asteraceae) is traditionally used as a potent anti-inflammatory
medicinal plant. The commercial petal samples of marigold are mostly used as herbal tea for its
sedative and oral health effects due to its anti-viral and anti-bacterial properties. Its significant
pharmacological activities are linked to the presence of distinct classes of compounds,
particularly phenolic compounds, carotenoids, volatile oil, terpenoids and flavonoids. Taking into
consideration that the amount and type of constituents could be variable in the commercial
samples provided from Cyprus and Turkey compared to the standard due to various factors
such as ecosystem and climate, time of harvesting, drying procedure and soil type, in this article
the quality and safety of market samples was determined while ethanol and hexane extracts of
standard and commercial C. officinalis samples were investigated for their anti-radical activity
using ABTS and DPPH method. They both demonstrated dose-dependent manner anti-radical
activity, further investigation by TPC (Total Phenolics Content) and HPLC provided evidence that
both commercial and standard samples present similar fingerprint in terms of composition, and
the total phenol equivalent to Gallic acid is not significantly different (P<0.05); therefore, both
samples can be considered to be good and safe antiradical candidates used alternatively with
the pharmacopeial drug. © 2022 Marmara University Press.
KW - ABTS-4
KW - Calendula officinalis-1
KW - DPPH-3
KW - HPLC-5
KW - phenolics-2
KW - alcohol
KW - antioxidant
KW - benzoic acid derivative
KW - caffeic acid
KW - Calendula extract
KW - Calendula officinalis extract
KW - carotenoid
KW - catechin
KW - chlorogenic acid
KW - cinnamic acid
KW - coumaric acid
KW - ferulic acid
KW - flavonoid
KW - flavoxanthin
KW - gallic acid
KW - hexane
KW - lutheoxanthin
KW - lycopene
KW - polyphenol derivative
KW - quercetin
KW - rosmarinic acid
KW - rutoside
KW - syringic acid
KW - unclassified drug
KW - ABTS radical scavenging assay
KW - antioxidant activity
KW - Article
KW - Calendula officinalis
KW - chemical composition
KW - comparative study
KW - controlled study
KW - DPPH radical scavenging assay
KW - drug isolation
KW - drug mechanism
KW - ethnopharmacology
KW - IC50
KW - market
KW - nonhuman
KW - petal
KW - phytochemistry
KW - reversed phase high performance liquid chromatography
KW - standard
PB - Marmara University
SN - 26306344 (ISSN)
LA - English
J2 - J. Res. Pham.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: M. Koşar; Eastern
Mediterranean University, Faculty of Pharmacy, Famagusta, North Cyprus via Mersin-10,
Turkey; email: muberra.kosar@emu.edu.tr
ER -

TY - JOUR
AU - Zeinsteger, P.A.
AU - Barberón, J.L.
AU - Leaden, P.J.
AU - Palacios, A.
TI - Antioxidant properties of Calendula officinalis L. (Asteraceae) on Fe2+-initiated
peroxidation of rat brain mitochondria
PY - 2018
T2 - Medicinal Chemistry Research
VL - 27
IS - 11-12
SP - 2523
EP - 2529
DO - 10.1007/s00044-018-2254-1
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055870125&doi=10.1007%2fs00044-0
18-2254-1&partnerID=40&md5=236722dec4529bbb303d447f38208751
AD - Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La
Plata, La Plata, Argentina
AB - In the study reported here, the effect of Calendula officinalis L. (Asteraceae) extract (CO)
on the polyunsaturated fatty acid composition, chemiluminescence, and Peroxidizability Index
(PI) of mitochondria isolated from brain rat was analyzed. After incubation of mitochondria in an
ascorbate (0.4 mM)-Fe++ (2.15 µM) system (180 min at 37 °C), it was observed that the total
cpm/mg protein originated from light emission: chemiluminescence was lower in brain
mitochondria obtained from CO group than in the control group (without extract
supplementation). Moreover, it was observed that the extract was reduced, concentration
dependent, of chemiluminescence, measured as total cpm. The fatty acid composition of brain
mitochondria from control group was profoundly modified when subjected to non-enzymatic
lipoperoxidation with a considerable decrease of arachidonic acid C20:4ω6 and
docosahexaenoic acid C22:6ω3. As consequence, the PI, a parameter based on the maximal
rate of oxidation of specific fatty acids was higher in the CO group than in the control group. The
simultaneous analysis of PI, chemiluminescence, and fatty acid composition indicates that CO
may act as an antioxidant protecting rat brain mitochondria from peroxidative damage. © 2018,
Springer Science+Business Media, LLC, part of Springer Nature.
KW - Calendula officinalis L. (Asteraceae)
KW - Chemiluminescence
KW - Mitochondria
KW - Peroxidation
KW - arachidonic acid
KW - ascorbic acid
KW - Calendula extract
KW - docosahexaenoic acid
KW - ferrous ion
KW - flavonoid
KW - phytochemical
KW - polyunsaturated fatty acid
KW - protein
KW - animal tissue
KW - antioxidant activity
KW - Article
KW - Asteraceae
KW - brain mitochondrion
KW - Calendula officinalis
KW - chemoluminescence
KW - controlled study
KW - fatty acid analysis
KW - female
KW - lipid composition
KW - lipid peroxidation
KW - medicinal plant
KW - nonhuman
KW - phytochemistry
KW - rat
PB - Birkhauser Boston
SN - 10542523 (ISSN)
LA - English
J2 - Med. Chem. Res.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 1; Correspondence Address: A. Palacios;
Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata,
La Plata, Argentina; email: apalacios@fcv.unlp.edu.ar; CODEN: MCREE
ER -

TY - JOUR
AU - Khpalwak, W.
AU - Abdel-dayem, S.M.
AU - Sakugawa, H.
TI - Individual and combined effects of fluoranthene, phenanthrene, mannitol and sulfuric acid
on marigold (Calendula officinalis)
PY - 2018
T2 - Ecotoxicology and Environmental Safety
VL - 148
SP - 834
EP - 841
DO - 10.1016/j.ecoenv.2017.11.065
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85036575605&doi=10.1016%2fj.ecoenv.2
017.11.065&partnerID=40&md5=22d865317fb705ebdcf87b026bb1ac55
AD - Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama,
Higashi-Hiroshima, 739-8521, Japan
AD - Department of Plant Protection, Faculty of Agriculture, Nangarhar University, Nangarhar,
Afghanistan
AD - Department of Pesticide Chemistry, Faculty of Agriculture, Kafrelsheikh University,
Kafrelsheikh, Egypt
AB - A study was conducted to characterize marigold stress response to polycyclic aromatic
hydrocarbons (PAHs) (oxidative stress inducers) with and without sulfuric acid (S.Acid; pH 3)
(acid-stress inducer), and to evaluate reactive oxygen species (ROS) scavenging activity of
mannitol (Mann). Marigold (Calendula officinalis) seedlings were grown in a greenhouse and
fumigated with fluoranthene (FLU), phenanthrene (PHE), Mann, and S.Acid individually and in
various combinations for 40 days. Various physiological and biochemical parameters among
others were analyzed using standard methods. The results revealed that fumigation of FLU
induced oxidative stress to the plants via ROS generation leading to negative effects on
photosynthesis at near saturating irradiance (Amax), stomatal conductance (Gs), internal
carbon dioxide concentration (Ci), leaf water relations and chlorophyll pigments. Significant per
cent inhibition of Amax (54%), Gs (86%) and Ci (32%), as well as per cent reductions in
chlorophyll a (Chl.a) (33%), Chl.b (34%), and total chlorophyll (Tot. Chl) (48%) contents were
recorded in FLU fumigated treatment in comparison to control. Combination of Mann with FLU
scavenged the generated ROS and substantially lowered the oxidative stress on the plants
hence all the measured parameters were not significantly different from control. PHE fumigation
had varied effects on marigold plants and was not as deleterious as FLU. Combined fumigation
of S.Acid with both the PAHs had significant negative effect on leaf water relations, and positive
effect on fresh and turgid weight of the plants but had no effect on the other measured
parameters. The lowest proline contents and highest catalase and ascorbate peroxidase
activities in FLU fumigated plants further confirmed that oxidative stress was imposed via the
generation of ROS. From the results, it is evident that Mann could be an efficient scavenger of
ROS-generated by FLU in the marigold plants. We recommend Mann to be widely used for the
protection of higher plants from FLU-generated stress in the urban areas. © 2017 Elsevier Inc.
KW - Antioxidant enzyme activities
KW - Fluoranthene
KW - Marigold
KW - Oxidative stress
KW - Reactive oxygen species
KW - Calendula
KW - Chlorophyll
KW - Drug Synergism
KW - Fluorenes
KW - Fumigation
KW - Mannitol
KW - Oxidative Stress
KW - Phenanthrenes
KW - Photosynthesis
KW - Polycyclic Aromatic Hydrocarbons
KW - Reactive Oxygen Species
KW - Sulfuric Acids
KW - Calendula officinalis
KW - Embryophyta
KW - ascorbate peroxidase
KW - carbon dioxide
KW - catalase
KW - fluoranthene
KW - mannitol
KW - phenanthrene
KW - proline
KW - reactive oxygen metabolite
KW - sulfuric acid
KW - chlorophyll
KW - fluoranthene
KW - fluorene derivative
KW - mannitol
KW - phenanthrene
KW - phenanthrene derivative
KW - polycyclic aromatic hydrocarbon
KW - reactive oxygen metabolite
KW - sulfuric acid
KW - angiosperm
KW - enzyme activity
KW - fluoranthene
KW - oxidative stress
KW - PAH
KW - phenanthrene
KW - reactive oxygen species
KW - scavenging (chemistry)
KW - sulfuric acid
KW - Article
KW - Calendula officinalis
KW - chlorophyll content
KW - controlled study
KW - dry weight
KW - enzyme activity
KW - fresh weight
KW - fumigation
KW - leaf water potential
KW - nonhuman
KW - oxidative stress
KW - pH
KW - photosynthesis
KW - plant parameters
KW - plant stress
KW - plant water content
KW - seedling
KW - stomatal conductance
KW - turgid weight
KW - Calendula
KW - drug effects
KW - drug potentiation
KW - fumigation
KW - growth, development and aging
KW - metabolism
KW - oxidative stress
PB - Academic Press
SN - 01476513 (ISSN)
C2 - 29197798
LA - English
J2 - Ecotoxicol. Environ. Saf.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 13; Correspondence Address: H. Sakugawa;
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 1-7-1
Kagamiyama, 739-8521, Japan; email: hsakuga@hiroshima-u.ac.jp; CODEN: EESAD
ER -

TY - JOUR
AU - Ebrahimi, E.
AU - Kheirollah, A.
AU - Mansouri, E.
AU - Babaahmadi-Rezaei, H.
AU - Mohammadzadeh, G.
TI - Effects of hydroalcoholic flower extract of marigold (calendula officinalis) on the biochemical
and histological parameters in stz-induced diabetic rats
PY - 2019
T2 - Jundishapur Journal of Natural Pharmaceutical Products
VL - 14
IS - 3
C7 - e55456
DO - 10.5812/jjnpp.55456
UR -
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071526181&doi=10.5812%2fjjnpp.554
56&partnerID=40&md5=dd41298556a7975736d35208ee235331
AD - Department of Clinical Biochemistry, Diabetes Research Center, Ahvaz Jundishapur
University of Medical Sciences, Ahvaz, Iran
AD - Department of Clinical Biochemistry, Cellular and Molecular Research Center, Ahvaz
Jundishapur University of Medical Sciences, Ahvaz, Iran
AD - Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of
Medical Sciences, Ahvaz, Iran
AD - Department of Clinical Biochemistry, Hyperlipidemia Research Center, Ahvaz Jundishapur
University of Medical Sciences, Ahvaz, Iran
AB - Background: Calendula officinalis, an aromatic plant and rich of various chemical
compounds, has been widely used in folk medicine for the treatment of different diseases.
Objectives: We investigated the effects of hydroalcoholic flower extract of Calendula officinalis
on the biochemical and histological parameters in STZ-induced diabetic rats. Methods: Thirty-six
male Wistar rats were divided into four groups: Healthy control, diabetic control, diabetic treated
with 250 and 500 mg/kg of the hydro-alcoholic extract, respectively. The biochemical and
histological measurements were assessed by standard methods. Results: The oral
administration of the hydroalcoholic extract of marigold flower resulted in a recovery of body
weight and significantly decreased fasting blood sugar (FBS) (P < 0.05). Alanine
aminotransferase (ALT), albumin and total bilirubin concentration were significantly recovered
after treatment with the extract (P < 0.05). Measurement of homeostasis model assessment for
β-cell function (HOMA-β) indicated that the reduction of serum insulin concentration after
treatment with the extract may be due to the recovery of beta cell function. Moreover, the extract
induced insulin secretion from the rat's isolated pancreatic islets under a glucose-stimulated
condition in a dose-dependent manner. The STZ-induced complications on the pancreas,
kidneys, and liver were improved after treatment with the extract. Conclusions: Our findings
suggest that the potential protective effects of marigold flower extract against the
diabetic-induced complicationsmaybe mediated by its effect on the reduction of blood glucose
and its direct effect on the glucose-stimulated insulin secretion. © 2019, Jundishapur Journal of
Natural Pharmaceutical Products.
KW - HOMA-β
KW - Insulin
KW - Marigold
KW - Streptozotocin
KW - alanine aminotransferase
KW - albumin
KW - alcohol
KW - antidiabetic agent
KW - aspartate aminotransferase
KW - bilirubin
KW - bilirubin glucuronide
KW - Calendula officinalis extract
KW - collagenase
KW - flower extract
KW - glucose
KW - insulin
KW - lactate dehydrogenase
KW - reduced nicotinamide adenine dinucleotide
KW - serum albumin
KW - unclassified drug
KW - adult
KW - animal experiment
KW - animal model
KW - animal tissue
KW - antidiabetic activity
KW - Article
KW - biochemical analysis
KW - body weight
KW - Calendula officinalis
KW - cell function
KW - clinical assessment
KW - clinical evaluation
KW - concentration process
KW - controlled study
KW - diabetes control
KW - diabetic complication
KW - drug effect
KW - fasting
KW - glucose blood level
KW - histopathology
KW - homeostasis model assessment
KW - in vitro study
KW - insulin blood level
KW - insulin release
KW - kidney tissue
KW - liver tissue
KW - male
KW - nonhuman
KW - pancreas islet
KW - pancreas islet cell function
KW - pancreas tissue
KW - plant leaf
KW - rat
KW - streptozotocin-induced diabetes mellitus
KW - treatment indication
PB - Kowsar Medical Institute
SN - 17357780 (ISSN)
LA - English
J2 - Jundishapur J. Nat. Pharm. Prod.
M3 - Article
DB - Scopus
N1 - Export Date: 18 October 2023; Cited By: 2; Correspondence Address: G.
Mohammadzadeh; Department of Clinical Biochemistry, Hyperlipidemia Research Center,
Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; email:
mohammadzadeh@ajums.ac.ir
ER -