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ch.05 Column Chromatography

Chromatography is a separation technique first described by Mikhail Tswett in 1906, based on the movement of components between a stationary and a mobile phase. It includes various types such as ion exchange, adsorption, and size exclusion chromatography, each with specific principles and applications. Ion exchange chromatography specifically involves the exchange of ions between a solid stationary phase and a liquid mobile phase, useful for separating ions and compounds with similar properties.

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40 views9 pages

ch.05 Column Chromatography

Chromatography is a separation technique first described by Mikhail Tswett in 1906, based on the movement of components between a stationary and a mobile phase. It includes various types such as ion exchange, adsorption, and size exclusion chromatography, each with specific principles and applications. Ion exchange chromatography specifically involves the exchange of ions between a solid stationary phase and a liquid mobile phase, useful for separating ions and compounds with similar properties.

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Aman Ansari
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chapter ... S ia << ano The name chromatography is based on the Column Chr omatography far wring because the mathod was fist used for the separation of coloured substances found in CONTENTS| plants. This method was described by Mikhail 5.1 Introduction Tswett in 1906. 51.1 Chromatography Mike Tawett | 1.2 Principle of Chromatography 5.4.1 Chromatography 5.1.3” Types of Chromatography It is a technique employed for separation of components of mixture 5.2. lon Exchange Chromatography by continuous distribution of components between two phases i.e. one 5.2.1 Introduction phase moves (mobile) over the other (stationary) in a continuous manner. 5.2.2 Principle, mechanism When the chromatography is carried out in a column, it is called column | ‘hromatography. 5.2.3: Classification of Ion Exchange Resins (ation 4 Anion excharyer) Garomatograply: 5.2.4 Experimental Technique Ce coer 5.2.5 Applications of lon Exchange Chromatography a | 5.3 Adsorption Chromatography "Mixture of —[25) i 5.3.1 Column Chromatography compounds | 532 Principle Adsorbent —+| 5.3.3 Preparation of Column St hase! — ‘ pane (Stationary phase) 5.4 Applications of Column Chromatography (Antracene purification) 5,5. Size Exclusion Chromatography -mechenism, principle cies eal Question Bank Plug Fig. 5.1 : Column chromatography 5.1.2 Principle of Chromatography The technique of chromatography is based on the difference in rates 5.4 INTRODUCTION Some materials appear homogeneous, but are actually a combination of substances. For example, green plants contain a mixture h at which the components of a mixture move through a porous medium i tare used in Sidifagest ameter metan, the Bi i my (ale tatanary phese) under the nuance of some Hgu or o35 (alle this experiment, is a mixture 0! < ae mobile phase). Thus, this technique consists of two phases - one is a instances, we can separate these materi Is by dissolving them in an stationary phase of large surface area while the second is a moving phase appropriate liquid and allowing them to move through an absorbent llowed to move slowly over the stationary phase. The stationary. id id or a liquid while the moving phase may be a e paper. Chromatography is a method used by scientists for phase is either a s separating organic and inorganic compounds so that they can be | oragas. analysed and studied. Chromatography is a modern method used for the 5.1.3 Types of Chromatography separation of mixtures into its components, purification of compounds Depending upon the nature of the stationary or mobile phases, different types of chromatographic techniques commonly/

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