Lecture hours Small group teaching/tutorials/ Self-directed learning (hours) Total (hours)

integrated teaching/ practical

(hours)
80 150 20 250

DEPARTMENT OF BIOCHEMISTRY
 ANATOMY PHYSIOLOGY BIOCHEMISTRY
st
Syllabus to be taught before the 1 Internal Assessment Examination
GENERAL ANATOMY, GENERAL PHYSIOLOGY, CELL, ENZYMES,HEMOGLOBIN STRUCTURE & FUNCTION, ABNORMAL
UPPER LIMB, NERVE MUSCLE HEMOGLOBIN, VITAMINS, protein chemistry, IRON, CALCUIM, PLASMA
GENERAL HISTOLOGY, PHYSIOLOGY, PROTEINS, BIOLOGICAL OXIDATION, INTERMEDIARY METABOLISM
GENERAL EMBRYOLOGY, HEMATOLOGY, ENDOCRINE INTRODUCTION,HORMONES
LOWER LIMB. SYSTEM

1st INTERNAL ASSESSMENT –2nd WEEK OF DECEMBER 2019

(Dates shall be informed by the University in advance.)
Syllabus to be taught before the 2nd Internal Assessment Examination
HEAD & NECK, BRAIN CNS, RENAL SYSTEM, ACID- CARBOHYDRATE CHEMISTRY & METABOLISM, PROTEIN METABOLISM, LIPID CHEM
BASE BALANCE & METABOLISM, WATER ELECTROLYTE, ACID-BASE BLANCE

2nd INTERNAL ASSESSMENT –3rd WEEK OF MARCH 2020-16th

(Dates shall be informed by the University in advance.)
*The Practical Exams for the internal assessment may be completed before the theory examinations of the internal assessment
as per the convenience of the institution.
Syllabus to be taught before the 3rd Internal Assessment Examination
THORAX, ABDOMEN, CARDIOVASCULAR SYSTEM, NUCLEOTIDE CHEMISTRY & METABOLISM, , INTEGRATION OF METABOLISM,
PELVIS, PERINEUM RESPIRATORY SYSTEM, GIT, MOLECULAR BIOLOGY, ECM, ORGAN FUNCTION TESTS,CARCINOGENESIS,
REPRODUCTIVE FREE RADICALS, DETOXIFICATION, IMMUNOLOGY, NUTRITION, MINERAL
PHYSIOLOGY, INTEGRATED METABOLISM.
PHYSIOLOGY
PRE FINAL EXAM –2nd WEEK OF AUGUST 2020 (Dates shall be informed by the University in advance.)
*The portion for the Pre Final Exam (3rd Internal Assessment Exam) includes entire syllabus of the subject.
**The Practical Exams for the internal assessment may be completed before the theory examinations of the internal
assessment as per the convenience of the institution.

competency.n competency. Domain/ Objective

o level/core At the end of the session students should be able to

BI1.1 Describe the KH  Describe the structure & functions of the cell and
molecular and sub-cellular organelles
functional  Describe structure of cell membrane(fluid mosaic
organization of a model)
cell and its  specialized features of plamamembrane(lipid
subcellular rafts,Caveolae,tight junctions ,desmosomes)
components.  Describe transport mechanisms-(a)passive diffusion
(b)facillitated diffusion-transporters ,ion channels
(c)Active transport- (Na+ -K+ ATP ase )
 ionophores & aquaporins
  Describe about the transport defects and organelle
dysfunction
 Describe the composition of body fluids, distribution
of water.
BI7.1. Describe the KH  Define nucleosides &nucleotides and their
structure and biomedical importance
functions of DNA  Explain the Structure & composition of different
and RNA and nucleotides,
outline the cell  Describe the structure of DNA & RNA: different
cycle types and function
 Organization of DNA
 synthetic nucleotide analogues

BI5.1 . Describe and KH  All types of classification of the amino acids &
discuss structural Functions.
organization of  Importance of non standard amino acids
proteins.  properities of Amino acids--
zwitterions,pKa,isoelectric pH
 properities determined by α R chain and functional
groups (peptide bond), derivatives of Amino acids
 Explain the four levels of protein structure and other
bonds required to stabilize the protein structure
 Ramachandran plots
 Principles of determination of Protein structure
 role of Genomics & Proteomics
 classification of proteins
 physical and chemical properities proteins and
 biologically important peptides
 structural Proteins-- structure of
collagen,Elastin,Fibronectine,fibrillins,laminin,proteo
glycans & glycosaminoglycans ,
 associated Nutritional & other disorders of structural
proteins
.
 BI2.1.  Explain the KH  General characteristics and classification of Enzymes
fundamental prosthetic group,cofactors &coenzymes
concepts of  enzymes kinetics-- Gibbs free energy,activation
enzyme, energy
isoenzyme,  Factors effecting rate of enzyme catalysed
alloenzyme,c reaction ,Michaelis-menten's Equation &Hill's
oenzyme & equation (Km ,Vmax )
co-factors.  Regulation of Enzyme activity- compartmentation,
Enumerate rate-limiting reactions ,Induction& repression of
the main enzymes, Allosteric regulation, feed back
classes of regulation ,co-valent modification,proenzymes
IUBMBnome  Clinical Enzymology-- Isoenzymes, Diagnostic
nclature. Enzymes & Therapeutic enzymes.
 Enzyme inhibition -competitive ,non-competitive
inhibition with suitable examples & clinical
significance Enzyme inhibitors as poisons ,drugs.
 Interpretation of laboratory results of enzyme
activities
 Enzyme patterns in diseases – Liver, Heart
 Basic principles underlying mechanism of enzyme
 action
BI6.5. Describe the KH  Classification of vitamins
biochemical role of  Describe the biochemical functions of Vitamin - A
vitamins in the  Describe sources, RDA, deficiency manifestations of
body and explain Vitamin A
the manifestations  Describe the biochemical functions of Vitamin - D
of their deficiency  Describe sources, RDA, deficiency manifestations of
Vitamin D
 Describe the biochemical functions of Vitamin- E&K
 Describe sources, RDA, deficiency manifestations of
Vitamin E&K
 Describe biochemical functions of Thiamine
 Describe biochemical functions of Riboflavin
 Describe sources, RDA, deficiences of
Thiamine ,Riboflavin
 Describe biochemical functions Niacine
 Describe biochemical functions Pyridoxine
 Describe sources, RDA, deficiences of
Niacine ,Pyridoxine
 Describe biochemical functions folic acid, B12
 Describe sources, RDA, deficiences of folic acid, B12
 Describe biochemical functions biotin
 Describe biochemical functions of pantothenic acid
 water soluble vitamins - biotin ,pantothenic and and
sources, RDA, deficiencies
 vitamin like substances.& their biochemical functions
 Describe biochemical functions of Vit C
  Describe sources, RDA, deficiences of Vit C
BI6.6. Describe the KH  Describe the Laws of thermodynamics,high energy
biochemical compounds , ATP / ADP Cycle
processes involved  All types of oxidoreductases
in generation of  Redox Potentials
energy in  components of Respiratory chain / electron
cells. transport chain & synthesis of ATP
 Oxidative phosphorylation
 chemiosmatic theory
 Dicuss about the Inhibitors of respiratory chain ,
uncouplers of oxidative phosphorylation
 Substrate level phosphorylation
 shuttle mechanisms and Mitochondrial diseases
BI11.21 Demonstrate SH  Plasma Proteins-functions
estimation of  Plasma Proteins-biomedical importance.
glucose, creatinine,  Disorders of Plasma Proteins, Acte Phase Reactant
urea and total proteins.
protein in  electrophoretic pattern in different diseases
serum.
BI6.11. Describe the KH  Describe the structure function relationship of
functions of haem Hemoglobin &Myoglobin,.
in the body and  oxygen dissociation curve of
describe the Hemoglobin&Myoglobin,.
processes  Different Types of Normal hemoglobins,hemoglobin
involved in its Derivatives
metabolism and  significance of Bohr effect & 2,3- BPG.
describe porphyrin  hemoglobinopathies,abnormalhemoglobins and their
 metabolism biomedical implications
 Describe biosynthesis of heme.
 Classify Porphyrias (enzyme defect and symptoms).
 Catabolism of Heme :bilrubin
Metabolism ,transport., conjugation, secretion &
excretion of bilirubin.
 Normal levels of Bilirubin, causes, clinical features &
laboratory investigations for the different types of
jaundice.
 Congenital & acquired Hyperbilirubinemias.

BI6.9 Describe the KH  Describe Absorption , transport and storage of iron,

functions of functions of iron.
various minerals in  Mucosal block theory. Factors affecting absorption
the body, their  Discuss about the Normal Levels of iron in the body ,
metabolism Deficiency manifestations of iron Interpretation of
and homeostasis investigations
 Anemia
 Discuss about normal serum calicium levels, sources,
daily requirements, biochemical functions ,deficiency
manifestations and toxic effects of excessive calicum
levels
 calcium homeostasiby vitamin D ,parathyroid
hormone and calcitonin
 Describe the biochemical functions of
phosphorus,magnesium,sulfur in the body, & their
clinical signicance
  Describe the functions ofcopper,zinc, selenium,
flouride in the body& their clinical signicance
 Describe the functions of iodine,manganese,lithium
in the body, their metabolism & their clinical
signicance
 Enumerate and describe the biochemical functions &
disorders associated with sulphur, cobalt, chromium

Discuss and KH  Classification of carbohydrates

BI3.1. differentiate  characteristics of individual groups with examples
monosaccharides, Biological/Clinical significance
di-saccharides and  Functions and biomedical importance energy fuel
polysaccharides Structural , Storage
giving examples of  Membrane carbohydrates (GAG)– Glycobiology
main
carbohydrates as
energy fuel,
structural element
and storage in the
human body
BI3.2&3.3 3.2 . Describe the
processes involved  Describe and discuss the digestion and assimilation of
in digestion and KH carbohydrates from food
assimilation of  Glucose -Transporters , Maldigestion and Malbsorption
carbohydrates and of carbohydrates
storage.
Describe and
discuss the
digestion and
assimilation of
carbohydrates
from food
BI3.4,3.5&3.7 Define and  Describe the aerobic and anaerobic glycolysis
differentiate the Regulatory steps of glycolysis
pathways of KH  Energetics and inhibitors of glycolysis
carbohydrate  Rapoport-Leuberingcylce-
metabolism,  pyruvate dehydrogenase complex
 (glycolysis,  Discuss about Lactic acidosis
gluconeogenesis,  Discuss about substrates for Gluneogenesis
glycogen  Describe the pathway of gluconeogenesis, energetics
metabolism, HMP & regulation
shunt).  Discuss about the Inborn errors of Glycolysis and
•Describe and Gluconeogenesis- hemolytic anaemias and
discuss the hypoglycaemia
regulation,  Discuss about the concepts of Reciprocal regulation
functions and and Futile cycles/Substrate cycles
integration of  Describe the synthesis and breakdown of glycogen
carbohydrate along & it's regulation
with associated  Discuss about the glycogen storage disorder
diseases/disorders.  Describe HMP SHUNT pathway & it's significance
•Describe the  Glucose 6 phosphate dehydrogenase deficiency
common poisons  Discuss about the metabolism of other sugars -uronic
that inhibit crucial acid pathway, metabolism of fructose & Galactose
enzymes of
carbohydrate
metabolism (eg;
fluoride, arsenate)
 Describe the reactions of the TCA cycle, Energetics
BI3.6 . Describe and KH and production of reducing equivalents & regulation
discuss the concept  Inhibitors of TCA cycle
of TCA cycle as a  Explain the importance of vitamins in the citric acid
amphibolic cycle.
pathway and its  Amphibolic pathway and anapleurotic reactions
regulation.
 Discuss the KH  Explain about glucose Homeostasis in health and
mechanism and disease
BI 3.9. significance of  Diabetes mellitus
blood glucose  Discuss about the biochemistry of Complications of
regulation in health DM
and disease  Polyol Pathway &protein Glycation
 Diabetes mellitus- interpretation of lab results
Discuss and  Discuss and interpret laboratory results of analytes
BI3.8 &BI3.10 interpret associated with metabolism of carbohydrates.
KH
laboratory results
of analytes
associated with
metabolism of
carbohydrates .
•Interpret the
results of blood
glucose levels and
other laboratory
investigations
related to
disorders of
carbohydrate
metabolism.

Describe and KH  Classify lipids with examples & their Functions

BI4.1. discuss main  Discuss biomedical importance of lipids
 classes of lipids
(Essential/non-  Differentiation of essential & non-essential FA&
essential fatty clinical role of MUFA &PUFA
acids, cholesterol
and hormonal  Physical and chemical characteristics
steroids, of lipids
triglycerides, major  Phospholipids -classification & functions •Compound
phospholipids and lipids &steroids -physiological importance
sphingolipids)  Amphipathic lipids -membranes, micelles, liposomes
relevant to human & emulsions
system and their
major functions

KH  Describe the processes involved in digestion and

BI4.2. Describe the absorption of dietary lipids
processes involved  Describe the process by which Fatty acids
in digestion and transported in the blood, activated, Fatty acid
absorption of Oxidations role of carnitine ,
dietary lipids and  outline β-oxidation pathway &energetic
also the key  oxidation of fatty acids with odd number of carbon
features of their atoms ,verylong chain & unsaturated fatty acids
metabolism  Discuss about the Disorders of Fatty acid oxidation
 Describe the process of ketogenesis
  explain how ketone bodies serve as a fuel for
extrahepatic tissue(Ketolysis)
 regulation of ketogenesis
 Clinical Significance of Ketosis
 Discuss about theDenovo synthesis of fatty acids
concept of Multy Enzyme Complex& regulation
 Discuss about the Elongation & Unsaturation of fatty
acids
 Describe the process of cholesterol biosynthesis
transport ,role of LCAT,& regulation of intracellular
cholesterol concentrations
 Discuss about the Fate of Cholestrol: excretion
&Products formed from cholesterol
 clinical significance

•Explain the
BI4.3. &BI4.4 regulation of  Describe the Major groups of lipoproteins , their
lipoprotein structure ,apo-lipoproteins ,transport , metabolism
metabolism & of chylomicrons, VLDL
associated  Metabolism of LDL ,HDL
disorders  Disorders of lipoprotein metabolism
•Describe the  Discuss about the regulation of lipogenesis &lipolysis
structure and KH  Role of brown adipose tissue in the generation of
functions of body heat
lipoproteins, their  Atherosclerosis
functions  lipoproteins- interrelations & relations with
 interrelations & atherosclerosis
relations with
atherosclerosis

Interpret
BI4.5 &BI4.7 laboratory results  Interpret laboratory results of analytes associated
of analytes with metabolism of lipids& Lipid Profile
associated with
metabolism
oflipids KH
•Interpret
laboratory results
of analytes
associated with
metabolism of
lipids.
 outline the cyclooxygenase and lipooxygenase
BI4.6 pathways responsible for the formation of various
Describe the classes of Eicosanoids
therapeutic uses of  Describe the therapeutic uses of prostaglandins and
prostaglandins and inhibitors of eicosanoid synthesis
inhibitors of
KH
eicosanoid
synthesis.
  Describe the digestion and absorption of dietary
BI5.3. Describe the proteins
digestion and  Discuss about the disorders of Digestion &
absorption of Absorption
dietary proteins.  Discuss about the Amino acid Transporters- Meistres
KH Cycle Biomedical importance

BI5.4. Describe common  Describe role of transaminases , glutamate

disorders dehydrogenase, glutaminase in Ammonia
associated with Metabolism
protein  Describe the process of urea Cycle
metabolism  Discuss about the Metabolic disorders asssociated
with urea cycle
KH  outline the metabolic pathways of
glycine•specialized products formed and disorders
 Explain the metabolic pathways of aromatic amnio
acids ( Phe, Tyr, Trp)

 Discuss about the specialized products formed from

aromaticamnio acids ( Phe, Tyr, Trp)

 Discuss about the Metabolic disordersof of aromatic

amnio acids ( Phe, Try, Trp)
  •Explain the metabolic pathways of sulphur
containing amino acids

 • Discuss about the specialized products formed and

Metabolic disorders of sulphur containing amnio
acids

 Discuss about the metabolism of branched chain

amino acids and disorders of branched chain
aminoacid metabolism

 Discuss about the metabolic pathways of aliphatic,

basic, acidic and hydoxyamnio acids, Proline &
Associated Inborn errors

 Discuss about polyamine and biogenic amines

 inborn errors

BI5.5 Interpret  Interpret laboratory results of analytes associated

laboratory results with metabolism of proteins
of analytes KH
associated with
metabolism of
proteins.
 Describe the KH  Discuss about the water balance & Regulation
BI6.7. processes involved  Discuss about the Electrolyte balance & Regulation
in maintenance of  Discuss about the Water imbalance Dehydration and
normal pH, water overhydration (causes, symptoms)
& electrolyte  Describe the role of buffer Systems in maintenance
balance of body of Acid Base Balance
fluids and the  Discuss about the mechanisms involved in Acid Base
derangements Balance pH homeostasis
associated with  Henderson-Hasselbach equation.
these.  Describe the role of respiratory system and renal
system in maintaining pH
 Discuss about the ACID-BASE imbalance, the causes,
mechanisms, & Compensations.
 Discuss and interpret results of Arterial Blood Gas
(ABG) analysis in various disorders.
 Acid -base disorders
 Interpretation of results of Arterial Blood Gas (ABG)
Discuss and analysis invarious disorders
BI6.8 interpret results of
Arterial Blood Gas
(ABG) analysis in
various disorders.
 Discuss the  Introduction to intermediary metabolism
BI6.1. metabolic
. processes that take KH  Discuss about the integration of metabolism during
place in specific physiological conditions & pathological conditions
organs in the body
in the fed and  Discuss about the metabolic processes that take
fasting states. place in specific organs in the body in the fed and
fasting states.

BI6.2 Describe and  Discuss about the metabolic processes in which

discuss the KH nucleotides are involved.
metabolic  Biosynthesis of purine nucleotides ,explain salvage
processes in which pathway
nucleotides are  Biosynthesis of pyrimidine nucleotides
involved  Regulation of purine and pyrimidine biosynthesis
BI6.3.& Describe the KH  Explain the catabolism of purine and pyrimidine
common disorders  Describe the common disorders associated with
associated with nucleotide metabolism (Gout ,hereditary orotic
nucleotide aciduria, ADA deficiency( SCID)& other
metabolism immmunodeficiency disorders
Discuss the 
laboratory results  Discuss the laboratory results of analytes associated
BI6.4 of analytes KH with gout &LeschNyhan syndrome
associated with
gout &Lesch-Nyhan
syndrome

BI7.2 Describe the KH  Describe the steps and enzymes of DNA replication
processes involved in prokaryots and eukaryots
in replication &  Describe about the Inhibitors of DNA Replication
repair of DNA and  Discuss about the types of DNA damage
transcription&  DNA repair mechanisms and Human diseases of DNA
translation damage repair
mechanisms.  Describe the process of Transcription in prokaryotes
and eukaryotes
 DNA dependent RNA polymerase ,inhibitors of
transcription &Reverse transcriptase
 Describe the salient features of Genetic code
 Describe the process of translation prokaryotes and
eukaryotes
 Inhibitors of translation
BI9.3 Describe protein KH  Describe protein foldinding ,role of chaperons ,prion
targeting & sorting diseases
along with its  Describe protein targeting & sorting along with its
associated associated disorders
disorders
BI7.3. Describe gene KH  Discuss gene mutations--types ,clinical significance•
mutations and  Regulation of gene expression in prokaryotes -
basic mechanism induccer, repressor, enhancer, operon concept(Lac
of regulation of operon &tryptophan operon),
gene expression  gene amplification , gene rearrangement ,RNA
processing,alternate mRNA splicing
 Regulation of gene expression in eukaryote-Cis
acting regulatory sequences-promoters or
enhancers,silencers or repressors ,insulators &locus
control regions ,Trans acting regulatory sequences-
transcriptinmechinery ,DNA binding morifsof
transcription factors, coactivators, corepressors
 Discuss about the post transcriptional modifcations
 Post translation modifications
 epigenetic Regulation of Gene expression -chromatin
remodeling ,Histone methylation ,DNA methylation

Describe KH  Explain the basic procedures and methods involved

BI7.4 applications of in recombinant DNA technology & genetic
molecular engeineeringand their applications
technologies like  Explain the procedure of PCR ,blotting techniques
 recombinant DNA and their applications
technology, PCR in  Treatment of diseases with genetic basis-Gene
the diagnosis and therapy and stem cell biology
treatment of  Transgenic animals
diseases with
genetic basis
List the functions KH  List the functions and components of the
BI9.1.&BI9.2. and components of extracellular matrix (ECM).
the extracellular
matrix (ECM).

Discuss the
involvement of  Diseases associated with connective tissue proteins
ECM components  Discuss the involvement of ECM components in
in health and health and disease
disease.
Describe the KH  classify hormones (Group I & Group II hormones )
BI6.13 , BI6.14 functions of the  Describe the mechanism of action of hormones
& 6.15. kidney, liver,  Explain the role of receptors amd GTP binding G
thyroid and proteins in hormone signal transduction
adrenal glands.  second messengers- c AMP, c GMP, Calicium and
phosphatidyl inositol
Describe the tests  Discuss about the functions of the thyroid gland
that are commonly and thyroid functional tests
done in clinical KH  Describe the functions, synthesis and regulation of
practice to assess Adrenocortical hormones
the functions of  Discuss the Adrenal function tests
 these organs  Disorders of Adrenal gland and interpretation of
(kidney, liver, Adrenal function tests
thyroid and  Describe the functions of the kidney
adrenal glands  Discuss about the Renal function tests
 Discuss about the disorders of Kidney and
Describe the KH interpretation of renal function tests
abnormalities of  Describe the functions of the liver •Discuss about
kidney, liver, the liver function tests
thyroid and  Disorders of Liver and interpretation of Liver
adrenal glands. function tests

BI10.1. Describe the KH  Describe the cancer initiation, promotion oncogenes

cancer initiation, & oncogeneactivation. Also focus on p53 & apoptosis
promotion  Describe various biochemical tumor markers and the
oncogenes & biochemical basis of cancer therapy.
oncogene  carcinoma-tumour markers
activation. Also
focus on p53 &
apoptosis
BI10.2
Describe various
biochemical tumor KH
markers and the
biochemical basis
of
cancer therapy
BI7.5. Describe the role KH  Describe the two phases of xenobiotic metabolism
of xenobiotics in (phase I -cyt P450& II reactions )
disease  Describe the role of xenobiotics in disease

BI7.6. & BI7.7 •Describe the anti-  Discuss the sources of ROS
KH
oxidant defence  Describe the role of oxidative stress in the
systems in the pathogenesis of conditions such as cancer
body.  complications of diabetes mellitus and
•Describe the role atherosclerosis.
of oxidative stress
in the pathogenesis
of conditions such
as cancer,
complications of
diabetes mellitus
and atherosclerosis
BI10.3. Describe the KH  Describe the types and structure of immunoglobulins
cellular and  properties of immunoglobulins &associated diseases
humoral  Monoclonal antibodies
components of the  Describe the cellular and humoral components of the
immune system & immune system
describe the types  Describe & discuss innate and adaptive immune
and structure of responses, self/non-self recognition and the central
antibody role of T-helper cells in immune responses.

BI8.1. Discuss the KH  Calorific value and Respiratory quotient of
importance of carbohydrates ,protein & lipids and its significance
various dietary  Define :BMR
components and  normal values & factors affecting BMR
explain importance  Define SDA
of dietary fibre  Discuss the importance of various dietary
components and explain importance of dietary fibre.
 Discuss the assessment of nutritional value of
proteins
 Discuss about nitrogen balance
BI8.2. Describe the types KH  Describe the types and causes of protein energy
and causes of malnutrition( Marasmus& kwashiorkor) and its
protein energy effects.
malnutrition and  Dietary advice for optimal health in childhood and
its effects. adult, in disease conditions like diabetes mellitus,
coronary artery disease and in pregnancy

BI8.3 & 8.4. Provide dietary KH  Dietary advice for optimal health in childhood and
advice for optimal adult, in disease conditions like diabetes mellitus,
health in childhood coronary artery disease and inpregnancy.
and adult, in  Describe the causes (including dietary habits), effects
disease conditions and health risks associated with being overweight/
like diabetes obesity.
mellitus, coronary  Discuss about the Glycemic index & it's significane
artery disease and
 inpregnancy.

Describe the KH
causes (including
dietary habits),
effects and health
risks associated
with being
overweight/
obesity.
BI8.5. Summarize the KH  Summarize the nutritional importance of commonly
nutritional used items of food including fruits and vegetables.
importance of (macro-molecules & its importance)
commonly used
items of food
including fruits and
vegetables.(macro-
molecules & its
importance)

List of Suggested text Books
Hapers Illustrated Biochemistry - V.Rodwell
Lippincotsillustrted reviews Biochemistry - R.Harvey
Marks Essentials of Biochemistry - M.Liberman
Clinical Chemistry - Marshall
Clinical chemistry and Metabolic Medicine- Crook
Biochemistry for Medical students - D.M Vasudevan

List of Reference text Books

Text book of Biochemistry with Clinical correlations - Devlyn
Biochemistry A case oriented aproach - Montgomery
Tietz Text book of Clinical chemistry and Molecular Diagnostics- C.A.Burtis
List of Biochemistry Practicals to be done by 1st MBBS student
QUALITATIVE EXPER IMENTS

Competency Experiment
BI-11.20 Composition of Normal Urine (DOAP)
BI-11.20 Abnormal Constituents of Urine, correlate these with Pathological
state --3 sessions(DOAP)
QUANTITATIVE EXPER IMENTS

Competency Experiment
BI-11.21 Estimation of Blood Glucose (DOAP)

BI-11.21 Estimation of Blood Urea (DOAP)

BI-11.21 &BI-11.7 Estimation of Serum Creatinine & Creatinine clearance(DOAP)
BI-11.21& BI-11.8 Estimation of Serum Total protein, Albumin & A/G ratio(DOAP)
BI-11.13 Estimation of SGOT/ SGPT
BI-11.14 Estimation of ALP
BI-11.9 Estimation of Serum Cholesterol & HDL
BI-11.10 Estimation of Triglycerides
BI-11.11 Estimation of Calcium and Phosphorus
BI-11.15 Composition of CSF
BI-11.12 Estimation of Serum Bilirubin

DEMONSTRATION/ CLINICAL CHARTS/CASE STUDIES

BI-11.1 Commonly used laboratory apparatus &Equipment , Good Safety Laboratory ,waste
disposal
BI-11.2 Preparation of Buffers & Estimation of pH, pH meter
BI-11.5 Paper chromatography of amino acids, TLC
&11.16
BI-11.16 Protein electrophoresis, PAGE, Immuno-diffusion
BI -11.6 Principle of Colorimetry& spectrophotometry
BI -11.18
BI-11.16 Auto analyser & Quality Control
BI-11.16 Electrolyte analysis by ISE, ABG Analyzer &ELISA
BI-11.17 Biochemical tests done in –
Diabetes Mellitus, Dyslipidemia , MI, Renal Failure, Nephrotic Syndrome,
Proteinuria, edema
BI-11.17 Biochemical tests done in –
Gout, Jaundice and Liver, Disease, Pancreatitis , Disorders of Acid-Base balance,
Thyroid disorders, vitamin deficiency disorders, mineral disorders
BI-11.23 A. Calculation of energy content of different food items
B. Identification of food items with high &low Glycemic Index
C. significance of food items in diet
BI-11.24 Advantage /disadvantage of use of unsaturated, saturated &trans fat in the food

It was suggested by the members that uniformity should be there in spotters, Methods of quantitative
experiments (kit methods)& spotters.

Methods suggested for quantitative experiments(kit methods):

1. Glucose –GOD-POD Method

2. Urea -- Urease Method
3. Creatinine –Jaffe’s Method
4. Total Proteins—Biuret Method
5. Albumin –BCG Method
6. Cholesterol –CHOD –POD Method
7. Triglycerides –GPO-PAP Method
8. SGOT/SGPT –IFCC Method
9. ALP – IFCC Method
10.Calcium – OCPC Method
11.Phosphorous - ModifiedMetol Method
12.Bilirubin –Jendrassik-Grof Method
CERTIFICATION

For the 4 quantitative experiments which

Topic Max Marks comes under DOAP,certification should be
marks obtained done before Pre-final practical examination
Identifies the test method 2 in the form of FORMATIVE
Explains the principle 2 ASSESSMENT.
Explains the procedure 2
Accurate use of micropipette 2
Does steps done in order 3
Takes the reading using semi 2
autoanalyser/colorimeter CHECK LIST FOR QUALITATIVE
Performs the given test with 3 EXPERIMENT
accurate value/close to true value
Interprets the result 2
Cautious of personal safety 2
Total 20
 Topic Max Marks
marks obtained
Identifies the test method 2
Explains the principle 2
Explains the procedure 2
Accurate use of micropipette 2
Does steps done in order 3
Takes the reading using semi 2
autoanalyser/colorimeter
Performs the given test with 3 CHECK LIST FOR QUANTITATIVE
accurate value/close to true value EXPERIMENT
Interprets the result 2
Cautious of personal safety 2
Total 20
Final Practical exam syllabus
QUALITATIVE EXPERIMENTS (DOAP)

1. Composition of Normal Urine

2. Abnormal Constituents of Urine

QUANTITATIVE EXPERIMENTS (DOAP)

1. Estimation of Blood Glucose

2. Estimation of Blood Urea
3. Estimation of Serum Creatinine & Creatinine clearance
 4. Estimation of Serum Total protein & A/G ratio

Final Practical exam question paper marks division

TYPE OF TEST marks

Qualitative 20
Quantitative 20
Interpretation of lab data(2X10) 20
Spotters 15
Record 5
THEORY VIVA 20
TOTAL 100