MEDICAL LABORATORY SCIENCE PROGRAM

MLS 051: COMMUNITY AND PUBLIC HEALTH

DONNING AND DOFFING OF PPEs

DONNING OF PERSONAL PROTECTIVE EQUIPMENT (PPE)

1. Ensure that a trained Observer is available to verify compliance with all steps.
2. Ensure that all the needed supplies are available.

ITEMS NEEDED:

a) Impermeable shoe covers

b) N95 respirator
c) Goggles
d) Plastic apron
e) Three sets gloves with extended cuffs
f) Freshly-prepared 1:10 bleach solution.
g) Alcohol-based handrub
h) Cover all

3. Prepare for donning of PPE

a) Wear scrubs
b) Wear plastic or rubber boots
c) Remove watch, jewelry and contents of the pockets
d) Secure hair
e) Inspect PPE items prior to putting on.
f) Ensure that all items are in good working condition
g) Make sure that the sizes are correct
INSTRUCTION FOR TRAINED OBSERVERS: Review the steps with the healthcare worker
before beginning the donning process,

4. Using a checklist and in a stepwise fashion, read the donning sequence to the
healthcare worker
5. Put on boot covers.
6. Perform hand hygiene.
a. Use alcohol-based hand rub Allow hands to dry before moving to the next step
7. Put on inner gloves.
8. Put on cover-all/gown.
9. Make sure that cover all is large enough for free movement
a. Make sure that the cuffs of inner gloves are tucked under sleeves of the coverall
b. Zip up cover-all
c. Make thumb holes at the border of cuff and sleeves. Use a scissors to make a
small incision
10. Put on N95 respirator.
a. Cup the respirator in your hand with the nosepiece at the fingertips Position the
respirator under your chin with the nosepiece up
b. Pull the bottom strap over our head and position it around the neck, below the
ears Pull the top strap over your head resting it high at the back of your head.
c. Mold the nosepiece using two fingers of each hand to the shape of your nose
d. Pinching the nosepiece using one hand may compromise the respirator
performance
11. Perform seal check.
a. Inhale deeply and feel the respirator slightly being sucked in
b. Exhale sharply and feel the respirator slightly bulge
12. Put on hood. Make sure that the hood covers all of hairs, ears, and neck with no skin
exposed
13. Put on outer apron.
a. Tie the apron with a simple knot for easier removal
14. Put on outer gloves. Ensure that the cuffs are pulled over the sleeves of the gown/cover-
all
15. Put on face shield or goggles.
 16. Let the trained observer check the worn PPE.
a. Make sure that all parts of skin are covered
b. All areas of the body should be covered during the range of movement
c. The healthcare worker should be able to extend his/her arms, bend at the waist,
and do range of motions

DOFFING OF PERSONAL PROTECTIVE EQUIPMENT (PPE)

KEY POINTS IN DOFFING PPE

1. PPE should be taken off in a designated PPE removal area

a. HIGH RISK ZONE

PPE should be taken off in a designated PPE removal area

HIGH RISK ZONE LOW RISK ZONE

2. Put all PPE in a leak-proof bag for infectious waste

3. Doff PPE only with supervision of a trained observer

RECOMMENDED PPE FOR TRAINED OBSERVER

1. Single-use full face shield and disposable face mask.

2. coverall, or a single-use fluid-resistant gown that extends below the knees.
3. single-use, double gloves with extended cuffs (at minimum, outer gloves should have
extended cuffs)
4. single-use fluld-resistant boots or impermeable shoe covers

INSTRUCTIONS FOR TRAINED OBSERVER

1. Remind the healthcare worker to avoid actions that may put them at risk (e.g. touching of
the face.
2. Read aloud each step of the procedure.
3. Let the healthcare worker repeat back the step they are performing.
4. Confirm visually that the PPE has been properly doffed.
5. Minimize touching the healthcare worker or his/her PPE during the process.
6. If contact with the health worker is made, the trained observer should immediately
disinfect outer-gloved hands with alcohol-based hand rub or 1:10 bleach solution.
1. Inspect PPE for visible contamination or tears
• In case of visible contamination, use 1:10 bleach disinfectant to wipe it off.
2. Disinfect outer gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
3. Remove apron
• Break the apron by pulling from the neck
• Avoid contaminating gloves by rolling the apron from inside out
4. Inspect the PPE again.
• Assess the PPE for visible contamination or tears.
• If with visible contamination, disinfect with 1:10 bleach disinfectant wipe.
5. Disinfect outer gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
6. Remove boot covers.
• Use a " hands-free" approach by stepping on the back of the cover and
lifting the foot Touch only the inner surface of the foot cover.
7. Disinfect outer gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
8. Remove outer gloves.
• Be careful not to contaminate the inner gloves when removing the outer
gloves.
9. Inspect and disinfect inner gloves.
• In case of contamination or tears, disinfect and remove gloves and do
hand hygiene on bare hands and put on a new pair of gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
10. Remove goggles.
• Lift back of strap over your head, pull out and away.
• Avoid touching the front surface of the goggles.
11. Disinfect inner gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
12. Remove hood.
 • Unfasten hood (if applicable). Grasp the back of the hood and carefully
remove by pulling backwards.
• Do not touch the inside of the hood.
13. Disinfect inner gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
14. Remove coverall
• Avoid contact of scrubs with outer surface of coverall
• Lift chin
• Find the zip at the level of your belly.
• Carefully trace your fingers to find the zip tab and fasteners with one
hand.
• Hold the top of the coverall with the other hand
• Unzip or unfasten completely before rolling down and turning inside -out
• Avoid contact of scrubs with the outer surface of the coverall. Touch only
inside of the coverall.
• Touch only the inside of the coverall when rolling it prior to disposal.
15. Disinfect Inner gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
16. Change inner gloves.
• Remove and discard gloves, taking care not to contaminate bare hands.
• Perform hand hygiene with alcohol-based hand rub.
• Put on a new pair of gloves.
17. Remove N95 respirator.
• Tilt the head slightly forward.
• Grab the bottom tie or elastic strap.
• Then grab the top tie or elastic strap.
• Remove without touching the front of the N95 respirator.
• Discard the N95 respirator.
18. Disinfect gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
19. Disinfect washable boots.
• Sit on a clean surface.
 • Starting at the top and finishing with the sole, wipe down the external
surface of the first boot.
• Use 1:10 bleach disinfectant. Set down the newly-cleaned boot in the
low-risk zone.
20. Disinfect and remove gloves.
• Use a 1:10 bleach disinfectant or an alcohol-based hand rub.
21. Perform hand hygiene.
• Use alcohol-based hand rub.
22. Inspect for any contamination.

Reminder after removal of PPE

✓ The healthcare worker can wear the dedicated washable boots and surgical scrubs in
the low risk area
✓ Healthcare workers entering high-risk areas should shower or at least change clothes
when leaving the low risk area.
✓ Clean the floor of the PPE removal area with disposable cloth soaked in 1:10 bleach
solution after PPE removal is complete.
✓ For Breaches in PPE Wash skin with soap and water. irrigate mucous membranes with
copious amounts of water or an eyewash solution.
▪ Report the incident immediately
 COLLEGE OF MEDICAL LABORATORY SCIENCE
COC-FA-MT2 COMMUNITY AND PUBLIC HEALTH FOR MEDICAL LABORATORY SCIENCE

DONNING AND DOFFING OF PPEs

Name: _____________________________________________________________________________
Section:___________________________________________________

# STEPS OR PROCEDURES 5 4 3 2 1
DONNING OF PPE
1. Ensure that a trained Observer is available to verify compliance with all
steps.
2. Ensure that all the needed supplies are available.
3. Prepare for donning of PPE
4. Using a checklist and in a stepwise fashion, read the donning sequence
to the healthcare worker
5. Put on boot covers.
6. Perform hand hygiene
7. Put on inner gloves
8. Put on cover-all/gown.
9. Make sure that cover all is large enough for free movement
Make thumb holes at the border of cuff and sleeves. Use a scissors to
make a small incision
10. Put on N95 respirator.
11. Perform seal check.
12. Put on hood
13. Put on outer apron.
14. Put on outer gloves.
15. Put on face shield or goggles.
16. Let the trained observer check the worn PPE
 COLLEGE OF MEDICAL LABORATORY SCIENCE
COC-FA-MT2 COMMUNITY AND PUBLIC HEALTH FOR MEDICAL LABORATORY SCIENCE

DONNING AND DOFFING OF PPEs

Name: _____________________________________________________________________________
Section:___________________________________________________

# STEPS OR PROCEDURES 5 4 3 2 1
DOFFING OF PPE
1. Inspect PPE for visible contamination or tears
2. Disinfect outer gloves.
3. Remove apron
4. Inspect the PPE again.
5. Disinfect outer gloves.
6. Remove boot covers.
7. Disinfect outer gloves.
8. Remove outer gloves.
9. Inspect and disinfect inner gloves.
10. Remove goggles.
11. Disinfect inner gloves.
12. Remove hood.
13. Disinfect inner gloves.
14. Remove coverall
15. Disinfect Inner gloves.
16. Change inner gloves.
17. Remove N95 respirator.
18. Disinfect gloves.
19. Disinfect washable boots.
20. Disinfect and remove gloves.
21. Perform hand hygiene.
22. Inspect for any contamination.