PATHOLOGY PRACTICAL 2
STOOL COMPLETE EXAMINATION
MATERIALS: Slides, slide cover, PH Strips, sample collecting container, Normal Saline, Iodine
EQUIPMENT: Microscope
PROCEDURE:
General Stool examination
General Stool Examination (GSE) is carried out in laboratories for various diagnostic purposes. Examination of
stool is very helpful in the diagnosis of disease of the gastrointestinal tract. Mostly a clean container which does
not contain any detergent or disinfectant is sufficient for all types of stool examinations including stool culture.
It consist the following tests:-
A.PHYSICAL EXAMINATION OF STOOL:
Sample should be examined immediately after collection. Samples left standing prolonged will deteriorate
helminthes, Ovum, other parasites and increase the numbers of monilia and bacteria which gives wrong results,
however the following aspects of stool should be examined:
(1) Quantity: the adult person excretions about 150-250 gm. /day of feces, about (1/3-1/2) of feces dry weight is
bacteria.
(2) Consistency and form: Normal stool is well formed. But in constipation(Dehydration) the stool is solid
(Hard) and the semi-solid (soft or loose) seen whentaking certain medications and laxatives. In abnormal cases
such as diarrhea anddysentery the stool appear liquid, or watery in nature. In cholera the stools have arice water
appearance. In cases of malabsorption of fats the stools are pale bulkyand semi-solid.
(3) Colour:
1-Normal colours of stools are light to dark brown due to the Presence of bilepigments.
2-Dark black: In cases with bleeding into the intestinal tract the stools become darktarry in nature due to the
formation of acid hematin , ﺻﺒﻐﺔ ﺗﺴﺘﺨﺮج ﻣﻦ اﻟﮭﯿﻤﻮﺟﻠﻮﺑﯿﻦ اﻟﻤﺆﻛﺴﺪifthe bleeding is in the small intestines. In case of
bleeding in large intestines orrectum stool color may be bright red due to fresh blood.
3-Red color: Resulted from eating certain colorful foods such as red beets.
4-Clay colour: The stool may be clay coloured due to absence of stercobilinogen inbiliary tract obstruction.
(4) Odor: The normal fecal odor of stool resulted from Indole and Skatol. Odor of stools may become offensive
in conditions like, Intestinal amoebiasis. In cases of bacillary dysentery and cholera the stools are not foul smelling
due to the absence of fecal matter.
(5) Blood:
1- The blood is present on the outer surface of the feces and this caused either by contamination from menstrual
cycle blood in women or bleeding hemorrhoids from the blood vessels.
2- Blood should be noted in stools if present as it is indicative of Ulceration or presence of any other pathology
like malignancy.
(6) Mucus: Is present in certain conditions like amoebic or bacillary dysentery.
(7) Parasite: Stools may contain adult helminthes. Nematodes like Ascaris are easily visible as their size is large.
Hook worms and Proglottids of cetodes may also present. These may be visible to the naked eye.
Demonstrator: Dr. Adil Khaliq
Lab: 02 Stool R/E
B. MICROSCOPIC EXAMINATION OF STOOL:
The laboratory diagnosis of most parasitic infections is by the demonstration of ova of the parasite in the stools
of the infected person. The stool is collected in a clean container. The stool can be examined by the following
techniques. (a) Wet mounts examination. (b) Iodine examination. (a) Saline wet mount examination: The
stool is emulsified in normal saline and a large drop is placed on a glass slide and is then covered with a cover
slide. Then examined under a light microscope, it is important to examine specimen under 10X objective lens at
first to observe large molecules, cells, ova and helminthes, then to the 40X objective to complete the test. It is
preferable to keep the condenser down and the intensity of the light low for proper visualization of the ova and
cysts. The thickness of the film should be such that one is able to see the printed letters of the newspaper through
it. (b) Iodine examination: Iodine preparation leads to better visualization of morphological details of ova and
cysts as it stains the glycogen in them. However it has the disadvantage that the live trophozoites of Entamoeba
histolytica and other live parasites cannot be seen as the iodine kills them. The examination instructions in normal
saline must be followed the same in iodine test.
Mustafa H. Talha
3 Microscopic examination include the following:
(1) Pus cells: Observed in stool the same procedure as in urine.
(2) RBCs: Observed in stool the same procedure as in urine.
(3) Monilia: Observed in stool the same procedure as in urine.
(4) Protozoa: (a) Entamoeba histolytica: To investigate the vegetative phase (trophozoite) and cyst, causing
amoebic dysentery disease. (b) Entamoeba coli: trophozoite + cyst Note: - most of children diarrhea less than 2
years cause by Entamoeba coli. (c) Giardia lamblia, trophozoite + cyst, Cause watery diarrhea disease in children,
especially. (d) Balantidium coli, trophozoite + cyst, causing Balantidiasis in colon.
(5) Worms : (a) Enterobius vermicularis (pinworm): investigating the eggs that are of convex and flat surface
and a pointed end. (b) Ascaris lumbricoides: investigating for eggs which characterized by the content of granular
yellow to Brown irregular albumin membrane. (c) Hookworm (Ancylostoma duodenale): investigating the eggs
where the egg yolk is divided and surrounded by a thin membrane. (d) Tapeworms, (Taenia solium): investigating
the worm pieces called (gravid segments or Proglottids) that comes out with the feces. (e) Schistosoma mansoni:
Investigating the eggs distinct by lateral spin.
Mustafa H. Talha
4 C. CHEMICAL EXAMINATION OF STOOL (a) pH: The pH of stools is acidic in amoebic dysentery and
is alkaline in bacillary dysentery. (b) Occult blood: Presence of blood in feces which is not apparent on gross
inspection and which can be detected only by chemical tests is called as occult blood. Causes of occult blood
present in a number of diseases including malignancy of the gastrointestinal tract. The reagents used are: 1-
Benzidine reagent: - Development of blue colour is indicative of presence of occult blood in the stool specimen.
2- Orthotolidine: Development of green colour Benzidine test is also highly sensitive and false-positive reactions
are common. Since bleeding from the lesion may be intermittent, repeated testing may be required. Causes of
False-positive Tests 1. Ingestion of peroxidase-containing foods like red meat, fish, poultry, turnips, horseradish,
cauliflower, spinach, or cucumber. Diet should be free from peroxidase-containing foods for at least 3 days prior
to testing. 2. Drugs like aspirin and other anti-inflammatory drugs, which increase blood loss from gastrointestinal
tract in normal persons. Causes of False-negative Tests 1. Foods containing large amounts of vitamin C. 2.
Conversion of all hemoglobin to acid hematin (which has no peroxidase-like activity) during passage through the
gastrointestinal tract.
Demonstrator: Dr. Adil Khaliq
Lab: 02 Stool R/E
