Curriculum for M.Sc.

BIOCHEMISTRY

OUTCOME BASED EDUCATION WITH CHOICE BASED CREDIT

SYSTEM REGULATIONS AND SYLLABUS
(with effect from Academic Year 2020 - 2021)

V.V.Vanniaperumal College for Women, Virudhunagar, established in 1962, offers 20

UG Programmes, 14 PG Programmes, 6 M.Phil. Programmes and 6 Ph.D. Programmes. The
curriculua for all these Programmes, except Ph.D. Programmes, have been framed as per the
guidelines given by the and University Grants Commission (UGC) & Tamil Nadu State
Council for Higher Education (TANSCHE) under Choice Based Credit System (CBCS) and
the guidelines for Outcome Based Education (OBE).
The Departments of Commerce, English, History, Mathematics, Biochemistry and
Tamil upgraded as Research Centres offer Ph.D. Programmes as per the norms and
regulations of Madurai Kamaraj University, Madurai and do not come under the purview of
CBCS.
A. CHOICE BASED CREDIT SYSTEM (CBCS)
The CBCS provides an opportunity for the students to choose courses from the
prescribed Courses. The CBCS is followed as per the guidelines formulated by the UGC. The
performance of students is evaluated based on the uniform grading system. Computation of the
Cumulative Grade Point Average (CGPA) is made to ensure uniformity in the evaluation system.

List of Programmes in which CBCS/Elective Course System is implemented

UG PROGRAMMES
Arts & Humanities : History (E.M. & T.M.), English, Tamil

Physical & Life Sciences : Mathematics, Zoology, Chemistry, Physics,

Biochemistry, Home Science - Nutrition and
Dietetics, Costume Design and Fashion,
Microbiology, Biotechnology, Computer
Science, Information Technology and
Computer Applications.

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 Curriculum for M.Sc. BIOCHEMISTRY

PG PROGRAMMES
Arts & Humanities : History, English, Tamil
Physical & Life Sciences : Mathematics, Physics, Biochemistry, Home Science
- Nutrition and Dietetics, Chemistry, Zoology,
Computer Science, Information Technology,
Computer Applications (MCA*)
Commerce & Management : Commerce, Business Administration (MBA*)
* AICTE approved Programmes

PRE-DOCTORAL PROGRAMMES (M.Phil.)

Arts & Humanities : History, English, Tamil
Physical & Life Sciences : Mathematics, Biochemistry
Commerce & Management : Commerce
OUTLINE OF CHOICE BASED CREDIT SYSTEM - PG
1. Core Courses
2. Project
3. Elective Courses
3.1 Discipline Specific Elective Courses (DSEC)
3.2 Non Major Elective Course (NMEC)
4. Online Course – Practice for CSIR NET – General Paper
5. Extra Credit Courses (Optional)

List of Non Major Elective Courses (NMEC) Offered

PG PROGRAMMES
Name of the Course Semester Department
History of Freedom Movement in India III History
(A.D. 1885 - 1947)
English for Job Aspirants III English
jkpOk gpwJiwfSk III Tamil
Taxation Concepts and Assessment III Commerce
Entrepreneurship III Business Administration
Mathematics for Competitive III Mathematics
Examinations
Digital Electronics III Physics
Chemistry for Competitive Examinations III Chemistry
Apiculture III Zoology
Nutrition and Health III Home Science - Nutrition and Dietetics
Clinical Biochemistry III Biochemistry
Web Programming III Computer Science
Fundamentals of Information Technology III Information Technology
Web Technology III Computer Applications

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B. OUTCOME BASED EDUCATION (OBE) FRAMEWORK

The core philosophy of Outcome Based Education rests in employing a student - centric
learning approach to measure the performance of students based on a set of pre-determined
outcomes. The significant advantage of OBE is that it enables a revamp of the curriculum based
on the learning outcomes, upgrade of academic resources, quality enhancement in research and
integration of technology in the teaching-learning process. It also helps in bringing clarity among
students as to what is expected of them after completion of the Programme in general and the
Course in particular. The OBE directs the teachers to channelize their teaching methodologies
and evaluation strategies to attain the Programme Educational Objectives (PEOs) and fulfill the
Vision and Mission of the Institution.
Vision of the Institution
The founding vision of the Institution is to impart Quality Education to the rural
womenfolk and to empower them with knowledge and leadership quality.
Mission of the Institution
The mission of the Institution is to impart liberal education committed to quality and
excellence. Its quest is to mould learners into globally competent individuals instilling in them
life-oriented skills, personal integrity, leadership qualities and service mindedness.
B.1 Programme Educational Objectives, Programme Outcomes and Programme Specific
Outcomes

It is imperative for the Institution to set the Programme Educational Objectives (PEOs),
Programme Outcomes (POs) and Course Outcomes (COs), consistent with its Vision and
Mission statements. The PEOs and the POs should be driven by the Mission of the Institution
and should provide distinctive paths to achieve the stated goals. The PEOs for each Programme
have to fulfill the Vision and Mission of the Department offering the Programme.
Vision of the Department of M.Sc. BIOCHEMISTRY
To empower our students with scientific knowledge and skills and to mold them with pioneering
spirit, forward thinking, leadership and collaborative approach.
Mission of the Department of M.Sc. BIOCHEMISTRY
•To handle scientific and research faculty of students through deep learning of Biochemistry for
employability in research, academia and pharmaceutical fields,
•To advance traditional boundaries, to motivate for research and entrepreneurship
•Committed to improving the scientific world today.

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B.1.1 Programme Educational Objectives (PEOs)

PEOs are broad statements that describe the career and professional achievements that the
Programme is preparing the graduates to achieve within the first few years after graduation.
PEOs are framed for each Programme and should be consistent with the Mission of the
Institution.
Programme Educational Objectives (PEOs) of M.Sc. BIOCHEMISTRY Programme
The students will be able to
● provide in-depth knowledge in the core areas of life sciences for industries, clinical,
research , pharmaceutical labs, and academia.
● instill the ability of entrepreneurship in research and diagnostics
● equip skillful attitude promoting lifelong learning to meet the ever evolving professional
demands by developing ethical , interpersonal and team skills
Key Components of Mission Statement PEO1 PEO2 PEO3
Employability in research, academia and pharmaceutical fields ✓ ✓ ✓
Motivation for research and entrepreneurship ✓ ✓ ✓
Committed to improving the scientific world today ✓ ✓ ✓
B.1.2 Programme Outcomes (POs)
POs shall be based on Graduate Attributes (GAs) of the Programme. The GAs are the
attributes expected of a graduate from a Programme in terms of knowledge, skills, attitude and
values. The Graduate Attributes include Disciplinary Knowledge, Communication Skills, Critical
Thinking, Problem Solving, Analytical Reasoning, Research Related Skills, Co-operation/Team
Work, Scientific Reasoning, Reflective Thinking, Information/Digital Literacy, Multicultural
Competence, Moral and Ethical Awareness/Reasoning, Leadership Qualities and Lifelong
Learning.
On successful completion of the Programme, the students will be able to
1 apply their in-depth domain knowledge and practical skills in interdisciplinary fields for
research-based endeavours, employment and entrepreneurship development.
(Disciplinary Knowledge)
2 communicate proficiently and confidently with the ability to present complex ideas in a
concise manner to assorted groups. (Communication Skills)

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3 identify, formulate and solve problems in a consistent and systematic way with updated
skills using modern tools and techniques. (Scientific Reasoning and Problem Solving)
4 analyze the data, synthesize the findings and provide valid conclusion by critical
evaluation of theories, policies and practices for the betterment of society. (Critical Thinking
and Analytical Reasoning)
5 explore and evaluate globally competent research methodologies to apply appropriately in
interdisciplinary research; Develop and sustain the research capabilities to meet the
emerging needs for the welfare of the society. (Research Related Skills)
6 use ICT to mould themselves for lifelong learning activities to face career challenges in
the changing environment. (Digital Literacy, Self - Directed and Lifelong Learning)
7 self-manage and function efficiently as a member or a leader in diverse teams in a
multicultural society for nation building. (Co-operation/Team Work and Multicultural
Competence)
8 uphold the imbibed ethical and moral values in personal, professional and social life for
sustainable environment. (Moral and Ethical Awareness)
B.1.3 Programme Specific Outcomes (PSOs)
Based on the Programme Outcomes, Programme Specific Outcomes are framed for each
PG Programme. Programme Specific Outcomes denote what the students would be able to do at
the time of graduation. They are Programme-specific and it is mandatory that each PO should be
mapped to the respective PSO.
On successful completion of M.Sc. BIOCHEMISTRY Programme, the students will be able to
PO 1: Disciplinary Knowledge
PSO 1.a : Apply the knowledge of theoretical and experimental approaches in
Biochemistry in research oriented Endeavour to unravel problems in health care with a scientific
basis of life process and will have an ability to provide solution to new problems.
PSO 1.b : Recognize the importance of bioethics, entrepreneurship and career oriented
skills, thus providing a strong foundation for both academic / industrial placements across the
country and globe as well as setting up entrepreneurial ventures.
PO 2: Communication Skills
PSO 2 : Communicate the knowledge of Biochemistry to address environmental,
intellectual, societal and ethical issues through case studies with effective communication.

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PO3: Scientific Reasoning and Problem Solving

PSO 3.a: Enrich their analytical and problem solving skills with regard to biochemical
principles of life processes and technologies for combating human diseases.
PSO 3.b: build up the capacity of decision making with regard to scientific progress,
personal development and career choice.
PO 4: Critical Thinking and Analytical Reasoning
PSO 4: Apply the knowledge of experimental approaches on designing experiments,
analysis, interpretation of data and synthesis of information to provide valid conclusions
PO 5: Research Related Skills
PSO 5: An ability to properly understand the technical aspects with research aptitude of
existing technologies that help in addressing the biological and medical challenges faced by
humankind by adhering the code of conduct of Biochemistry
PO 6: Digital Literacy, Self - Directed and Lifelong Learning
PSO 6 : Analyze and interpret the data using state-of the-art techniques with ICT and modern
tools in planning and executing projects in Biochemistry.
PO 7: Co-operation/Team Work and Multicultural Competence

PSO 7: Develop leadership qualities, team spirit and good interpersonal skills to work
effectively in diverse fields individually or as a team
PO 8: Moral and Ethical Awareness
PSO 8 : Follow the global standards of codes of conduct in Life science community and
practice the imbibed moral values in their profession and society to maintain sustainable
environment.
PO-PEO Mapping Matrix
Attainment of PEOs can be measured by a PO-PEO matrix. PEOs should evolve through
constant feedback from alumnae, students, industry, management, etc. It is mandatory that each
PEO should be mapped to at least one of the POs.

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PEOs PEO1 PEO2 PEO3

POs/PSOs

PO1/PSO1 ✔ ✔ ✔
PO2/PSO2 ✔ ✔ ✔
PO3/PSO3 ✔ ✔ ✔
PO4/PSO4 ✔ ✔ ✔
PO5/PSO5 ✔ ✔ ✔
PO6/PSO6 - ✔
✔
PO7/PSO7 - ✔ ✔
PO8/PSO8 ✔ ✔ ✔

B.1.4 Course Outcomes (COs)

Course Outcomes are narrow statements restricted to the course contents given in five units.
Course Outcomes describe what students would be capable of, after learning the contents of the
Course. They reflect the level of knowledge gained, skills acquired and attributes
developed by the students after learning of Course contents. COs are measurable, attainable and
manageable in number. COs contribute to attain POs in such a way that each CO addresses at
least one of the POs and also each PO is reasonably addressed by adequate number of COs.
It is important to determine the methods of assessment. A comprehensive assessment
strategy may be outlined using the revised Bloom‟s Taxonomy levels.

BLOOM’S TAXONOMY

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CO - PO Mapping of Courses
After framing the CO statements, the COs framed for each course is mapped with POs
based on the relationship that exists between them. The COs which are not related toany of the
POs is indicated with (-), signifying Nil. Measurement Mapping is based on Four Points Scale

[High (H), Medium (M), Low (L) and NilT(-)]. For calculating weighted percentage of contribution
of each Course in the attainment of the respective POs, the weights assigned for H, M and L are 3,
2 and 1 respectively.

CO-PO/PSO Mapping Table (Course Articulation Matrix)

PO/PSOs PO1/ PO2/ PO3/ PO4/ PO5/ PO6/ PO7/ PO8/

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8
COs
CO1

CO2

CO3

CO4

CO5

ELIGIBILITY FOR ADMISSION

The candidate should have passed in B.Sc. Biochemistry, Zoology, Botany, Microbiology,
Biotechnology (General or any Specialization) Degree from any recognized University.

DURATION OF THE PROGRAMME

The candidates shall undergo the prescribed Programme of study for a period of two
academic years (four semesters).
MEDIUM OF INSTRUCTION
English

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B.2 EVALUATION SCHEME

Internal Assessment External Examination Total

Components
Marks Marks Marks
Theory 40 60 100
Project 60 40 100

B.2.1 Core Courses, Discipline Specific Elective Courses & Non Major Elective Course
INTERNAL ASSESSMENT
Distribution of Marks Theory
Mode of Evaluation Marks
Periodic Test : 25
Assignment K5 Level : 5
Seminar : 10
Total : 40
Three Periodic Tests - Average of the best two will be considered
Two Assignments - Better of the two will be considered
Practical
Mode of Evaluation Marks
Test : 15
Model Examination 15
Performance : 10
Total : 40
Test - Better of two will be considered Model
Examination - Better of two will be considered Performance
- Attendance and Record

Question Pattern for Periodic Test Duration: 2 Hours

Types of Question No. of No. of Marks for Max.
Section Questions Questions to each Marks
be answered Question
A Multiple choice
Q.No.(1 - 5) 5 5 1 5
questions
B Internal Choice -
Q.No.(6-10) 5 5 5 25
Either Or Type
C
Internal Choice- 2 2 10 20
Q.No.(11-12)
Either Or Type
Total 50*
*
The total marks obtained in the Periodic Test will be calculated for 25 marks

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Question Pattern Duration: 3 Hours

Types of No. of No. of Marks Total
Section Question Questions Questions to for each Marks
be answered Question
A Multiple choice 5 5 1 5
Q.No.(1 - 5)
B Internal Choice- 5 5 5 25
Q.No.(6-10) Either Or Type
C Internal Choice- 5 3 10 30
Q.No.(11-15) Either Or Type
Total 60
B.2.2 Project

Project is compulsory for II PG Students in IV Semester.

Distribution of Marks
Mode of Evaluation Marks
Internal Assessment : 60
External Examination : 40
Total : 100

Evaluation Pattern (100 marks)

Internal Assessment (60marks) External Assessment (40 marks)

One Periodic Test Project Pre-Submission One Open online Project Viva Voce
Report Presentation Course related to Presentation
(20) (20) the Project (10) (30) (10)
(10)

B.2.3Online Course
Practice for CSIR NET - General Paper
Internal Examination only
- Online Test with Multiple Choice Questions will be conducted in III Semester.
- Model Examination will be conducted after two periodic tests.
Distribution of Marks
Mode of Evaluation Marks
Periodic Test : 40
Model Examination : 60
Total : 100
Two Periodic Tests - Better of the two will be considered

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B.2.4 Extra Credit Courses

● Extra credits are allotted for the completion of Open Online Courses offered by
MOOC to the maximum of 15 credits.
The Courses shall be completed within the first III Semesters of the
Programme.
The allotment of credits is as follows
4 weeks Course - 1 credit
8 weeks Course - 2 credits
12 weeks Course - 3 credits

EXTRA CREDIT COURSES (OPTIONAL) offered by the Department

Assessment by Internal Examiner only
Distribution of Marks
Question Pattern Duration: 3 Hours

Section Types of Question No. of No. of Marks Total

Questions Questions to for each Marks
be answered Question
A
Multiple Choice 9 9 1 9
Q. No.(1- 9)
B Internal Choice –
3 3 7 21
Q. No.(10 -12) Either or Type
C Open Choice
3 2 20 20
Q. No.(13-15)
Total 50

*The total marks obtained in the Test will be converted in to 100 Marks

ELIGIBILITY FOR THE DEGREE

The candidate will not be eligible for the Degree without completing the prescribed Courses of
study and a minimum of 50% Pass marks in all the Courses.

 No Pass minimum for Internal Assessment for other Courses.

 Pass minimum for External Examination is 27 marks out of 60 marks for Core

Courses, Discipline Specific Elective Courses and Non Major Elective Course.

 Pass minimum for Practice for SET/NET - General Paper is 50 Marks.

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ATTENDANCE
 The students who have attended the classes for 76 days (85%) and above are
permitted to appear for the Summative Examinations without any condition.
 The students who have only 60-75 days (66% - 84%) of attendance are permitted to
appear for the Summative Examinations after paying the required fine amount
and fulfilling other conditions according to the respective cases.
 The students who have attended the classes for 59 days and less – upto 45 days (50% -
65%) can appear for the Summative Examinations only after getting special permission
from the Principal.

 The students who have attended the classes for 44 days or less (<50%) cannot appear for the
Summative Examinations and have to repeat the whole semester.

 These rules are applicable to UG, PG and M.Phil. Programmes and come in
effect from 2020-2021 onwards.

 For Certificate, Diploma, Advanced Diploma and Post Graduate Diploma

Programmes, the students require 75% of attendance to appear for the

Theory/Practical Examinations.
B.3 ASSESSMENT MANAGEMENT PLAN

An Assessment Management Plan that details the assessment strategy both at the
Programme and the Course levels is prepared. The continuous assessment is implemented using
an assessment rubric to interpret and grade students.
B.3.1 Assessment Process for CO Attainment
Assessment is one or more processes carried out by the institution that identify, collect and
prepare data to evaluate the achievement of Course Outcomes and Programme Outcomes. Course
Outcome is evaluated based on the performance of students in the Continuous Internal
Assessments and in End Semester Examination of a course. Target levels of attainment shall be
fixed by the Course teacher and Heads of the respective departments.
Direct Assessment (Rubric based) - Conventional assessment tools such as Term
Test, Assignment, Quiz and End Semester Summative Examination are used.
Indirect Assessment - Done through Course Exit Survey.

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CO Assessment Rubrics

For the evaluation and assessment of COs and POs, rubrics are used. Internal
assessment contributes 40% and End Semester assessment contributes 60% to the total
attainment of a CO for the theory courses. For the practical courses, internal assessment
contributes 50% and Semester assessment contributes 50% to the total attainment of a CO. Once
the Course Outcome is measured, the PO can be measured using a CO-PO matrix.
CO Attainment
Direct CO Attainment

Course Outcomes of all courses are assessed and the CO - wise marks obtained by all the
students are recorded for all the assessment tools. The respective CO attainment level is
evaluated
based on set attainment rubrics.

Attainment Levels of COs

Assessment Methods Attainment Levels

Internal Assessment Level 1 50% of students scoring more than average marks
or set target marks in Internal Assessment tools
Level 2 55% of students scoring more than average marks
or set target marks in Internal Assessment tools
Level 3 60% of students scoring more than average marks
or set target marks in internal Assessment tools
End Semester Summative Level 1 50% of students scoring more than average marks
Examination or set target marks in End Semester Summative
Examination
Level 2 55% of students scoring more than average marks
or set target marks in End Semester Summative
Examination
Level 3 60% of students scoring more than average marks
or set target marks in End Semester Summative
Examination
Target Setting for Assessment Method
For setting up the target of internal assessment tools, 55% of the maximum mark is fixed
as target. For setting up the target of End Semester Examination, the average mark of the class shall
be set as target.

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Formula for Attainment for each CO

Attainment = Percentage of students who have scored more than the target marks

Number of Students who Scored more than the Target

Percentage of Attainment= x 100
Total Number of
Students
Indirect CO Attainment
At the end of each Course, an exit survey is conducted to collect the opinion of the
students on attainment of Course Outcomes. A questionnaire is designed to reflect the views of
the students about the attainment of Course Outcomes.
Overall CO Attainment= 75% of Direct CO Attainment + 25 % of Indirect CO Attainment

In each Course, the level of attainment of each CO is compared with the predefined targets. If
the target is not reached, the Course teacher takes necessary steps for the improvement to
reach the
target.
For continuous improvement, if the target is reached, the Course teacher can set the target as
a value greater than the CO attainment of the previous year.
B.3.2 Assessment Process for Overall PO Attainment

With the help of CO against PO mapping, the PO attainment is calculated. PO

assessment is done by giving 75% weightage to direct assessment and 25% weightage
to indirect assessment. Direct assessment is based on CO attainment, where 75% weightage
is given to attainment through End Semester examination and 25% weightage is given to
attainment through internal assessments. Indirect assessment is done through Graduate Exit Survey
and participation of students in Co-curricular/Extra curricular activities.

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PO Assessment Tools

Mode of Assessment Assessment Tool Description

Direct Attainment CO Assessment This is computed from the calculated CO

(Weightage -75%) Attainment value for each Course
Indirect Attainment Graduate At the end of the Programme, Graduate Exit
(Weightage - 25%) Exit Survey 10% Survey is collected from the graduates and it gives
the opinion of the graduates on attainment of
Programme Outcomes
Co-curricular / For participation in Co-curricular/Extra curricular
Extra curricular activities during the period of their study.
activities 15%
Programme Articulation Matrix (PAM)

Course Code Course Title PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Average Direct PO Attainment

Direct PO Attainment in percentage

Indirect Attainment of POs for all Courses

POs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Graduate Exit Survey

Indirect PO Attainment
Attainments of POs for all Courses
POs PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
Direct Attainment (Weightage - 75%)
Indirect Attainment (Weightage - 25%)
Overall PO Attainment

Overall PO Attainment= [75% of Direct PO Attainment +

25% of Indirect PO Attainment (Graduate Exit Survey
& Participation in Co- curricular and
Extracurricular activities)]

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Expected Level of Attainment for each of the Programme Outcomes

Pos Level of Attainment

Value >= 70% Excellent
Value > = 60 % and Value < 70% Very Good

Value > = 50 % and Value < 60% Good

Value > = 40% and Value < 50% Satisfactory
Value < 40% Not Satisfactory
Level of PO Attainment
Graduation Batch Overall PO Attainment Whether Expected Level of
(in percentage) PO is Achieved? (Yes/No)

B.3.3 Assessment Process for PEOs

The curriculum is designed so that all the courses contribute to the achievement of
PEOs. The attainment of PEOs is measured after 3 years of completion of the Programme
only through Indirect methods.

Target for PEO Attainment

Assessment Criteria Target (UG) Target (PG)
Record of Employment 25 % of the class strength 30 % of the class strength
Progression to Higher Education 40 % of the class strength 5 % of the class strength
Record of Entrepreneurship 2 % of the class strength 5 % of the class strength

Attainment of PEOs
Assessment Criteria & Tool Weightage
Record of Employment 10
Progression to Higher Education 20
Record of Entrepreneurship 10
Feedback from Alumnae 30
Feedback from Parents 10
Feedback from Employers 20
Total Attainment 100

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Expected Level of Attainment for each of the Programme Educational Objectives

POs Level of Attainment
Value >= 70% Excellent
Value >= 60 % and Value < 70% Very Good

Value >= 50 % and Value < 60% Good

Value >= 40% and Value < 50% Satisfactory
Value < 40% Not Satisfactory

Level of PEO Attainment

Graduation Batch Overall PEO Attainment Whether Expected Level of
(in percentage) PEO is Achieved? (Yes/No)

C. PROCESS OF REDEFINING THE PROGRMME EDUCATIONAL OBJECTIVES

The college has always been involving the key stake holders in collecting information
and suggestions with regard to curriculum development and curriculum revision. Based on the
information collected, the objectives of the Programme are defined, refined and are inscribed
in the form of PEOs. The level of attainment of PEOs defined earlier will be analyzed and will
identify the need for redefining PEOs. Based on identified changes in terms of curriculum,
regulations and PEOs, the administrative system like Board of Studies, Academic Council and
Governing Body may recommend appropriate actions. As per the Outcome Based Education
Framework implemented from the Academic Year 2020 -2021, the following are the Programme
Structure, the Programme Contents and the Course Contents of M.Sc. BIOCHEMISTRY
Programme.

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MASTER OF SCIENCE- BIOCHEMISTRY (7015)

Outcome Based Education with Choice Base Credit System
Programme Structure - Allotment of Hours and Credits
For those who join in the Academic Year 2020-2021

Semester Total
Number of
Components
I II III IV Hours
(Credits)
Core Course 6 (5) 5 (4) 6 (5) 6 (5) 23 (19)
Core Course 6 (5) 5 (4) 6 (5) 6 (5) 23 (19)
Core Course 6 (5) 5 (4) 6 (5) 6 (5) 23 (19)
Core Course Practical 6 (3) 6 (3) 6 (3) - 18 (9)
Core Course Practical - 4(2) - - 4(2)
Project - - - 12 (8) 12(8)
Discipline Specific Elective Course 6 (5) 5 (4) - - 11 (9)
Non Major Elective Course - - 5 (4) - 5 (4)
Online Course - - 1 (1) - 1 (1)
Total 30 (23) 30 (21) 30 (23) 30 (23) 120 (90)
Extra Credit Course (Optional) - - - - - Limited to a
MOOC maximum of
15 credits
Extra Credit Course (Optional) 2 2

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MASTER OF BIOCHEMISTRY
Programme Code – 7015
PROGRAMME CONTENT
M.Sc. BIOCHEMISTRY - SEMESTER I
S. No Components Title of the Course Course Hours Credits Exam. Marks
Code per Hours
Week Total
Int. Ext.
1 Core Course-1 Chemistry of 20PBCC11 6 5 3 40 60 100
Biopolymers
2 Core Course-2 Principles of 20PBCC12 6 5 3 40 60 100
Biochemical and
Biophysical
Techniques
3 Core Course-3 Biochemical and 20PBCC13 6 5 3 40 60 100
Environmental
Toxicology
4 Core Biochemical 20PBCC11P 6 3 6 40 60 100
Practical-1 Techniques and
Analysis Lab
5 DSEC-1 DSCE-Cellular 6 5 3 40 60 100
Biochemistry and 20PBCE11/
Virology/
Enzymes and Enzyme 20PBCE12/
Technology/
Dairy Biochemistry 20PBCE13
30 23 500
DSEC- Discipline Specific Elective Course

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M.Sc. BIOCHEMISTRY -SEMESTER II

S. No Components Title of Course Hours Credits Exam. Marks

the Code per Hours Int. Ext. Total
Cours Week
1 Core Course -4 Endocrinology 20PBCC21 5 4 3 40 60 100
and Metabolic
Regulation
2 Core Course -5 Microbial 20PBCC22 5 4 3 40 60 100
Biochemistry
and
Fermentation
3 Core Course -6 Molecular 20PBCC23 5 4 3 40 60 100
Biology and
Genetic
Engineering
4 Core Microbiology 20PBCC21P 6 3 6 40 60 100
Practical -2 and
Molecular
biology
5 Core Bioinformatics 20PBCC22P 4 2 3 40 60 100
Practical-3 Lab

6 DSEC-2 DSEC- 1. Plant 20PBCE21/ 5 4 3 40 60 100

Biochemistry/
2.
Bioinformatics 20PBCE22/
and
Nanotechnology
/ Molecular 20PBCE23
Mechanism of
30 21 600

DSEC- Discipline Specific Elective Course

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 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY - SEMESTER III

S.No Components Title of the Course Course Hours Credits Exam. Marks
. Code per Hours
Week Int. Ext. Total

1 Core Course-7 Immunochemistry 20PBCC31 6 5 3 40 60 100

2 Core Course-8 Biostatistics 20PBCC32 6 5 3 40 60 100
3 Core Course-9 Eukaryotic Gene 20PBCC33 6 5 3 40 60 100
Expression
4 Core Practical- Immunology and 20PBCC31P 6 3 6 40 60 100
4 Advanced
Biochemistry lab
5 NMEC NME - Clinical 20PBCN31 5 4 3 40 60 100
Biochemistry
(Basics)
6 Online Course Practice for 20PGOL32 1 1 - 100 - 100
CSIR NET -
General Paper
Total 30 23 600

NMEC: Non Major Elective Course

21 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY -SEMESTER IV

S.No Components Title of the Course Hours Credits Exam. Marks

Course Code per Hours
Week Int. Ext. Total

1 Core Course-10 Environmental 20PBCC41 6 5 3 40 60 100

Biochemistry
2 Core Course-11 Clinical 20PBCC42 6 5 3 40 60 100
Biochemistry
3 Core Course-12 Developmental 20PBCC43 6 5 3 40 60 100
Biology and
Genetics
4 Project Project Viva - 20PBCC41P 12 8 6 40 60 100
Voce R
30 23 400

22 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

MASTER OF BIOCHEMISTRY
Programme Code - 7015
REVISED PROGRAMME CONTENT M.Sc.
BIOCHEMISTRY -SEMESTER I

S.N Components Title of the Course Hours Cred Exam. Marks

o. Course Code per it Hours
Week s Int. Ext. Total
1 Core Course-1 Chemistry of 20PBCC11 6 5 3 40 60 100
Biopolymers
2 Core Course-2 Principles of 20PBCC12 6 5 3 40 60 100
Biochemical and
Biophysical
Techniques
3 Core Course-3 Biochemical and 20PBCC13 6 5 3 40 60 100
Environmental
Toxicology
4 Core Practical-1 Biochemical 20PBCC11P 6 3 6 40 60 100
Techniques and
Analysis Lab
5 DSEC-1 DSCE-Cellular 6 5 3 40 60 100
Biochemistry and 20PBCE11/
Virology/ 20PBCE12/
Enzymes and 20PBCE13
Enzyme
Technology/Dairy
Biochemistry
Total 30 2 500
DSEC- Discipline Specific Elective Course

23 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
SEMESTER II

S.No Components Title of the Course Course Hours Cred Exam Marks
. Code per its .Hours
Week Int. Ext. Total

1 Core Course -4 Endocrinology and 20PBCC21N 5 4 3 40 60 100

Metabolic Regulation
2 Core Course -5 Microbial Biochemistry 20PBCC22N 5 4 3 40 60 100
and
Fermentation
3 Core Course -6 Molecular Biology and 20PBCC23N 5 4 3 40 60 100
Genetic Engineering
4 Core Practical -2 Microbiology and 20PBCC21P 6 3 6 40 60 100
Molecular biology
Techniques Lab
5 Core Practical-3 Bioinformatics Lab 20PBCC22P 4 2 3 40 60 100
6 DSEC-2 DSEC- 1. Plant 20PBCE21N 5 4 3 40 60 100
Biochemistry/ /
2. Bioinformatics 20PBCE22/
and 20PBCE23
Nanotechnology/
Molecular
Total 30 21 600
Mechanism of
Extra Biosafety, 22PBCO21 2 100
7 Credit laboratory
Course safety and
(Optional) IPR

DSEC- Discipline Specific Elective Course

24 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY - SEMESTER III

S.No. Components Title of the Course Hours Credits Exam. Marks
Course
Code per Hours
Int. Ext. Total
Week

1 Core Course-7 Immunochemistry 20PBCC31 6 5 3 40 60 100

2 Core Course-8 Biostatistics 20PBCC32N 6 5 3 40 60 100

3 Core Course-9 Eukaryotic Gene 20PBCC33N 6 5 3 40 60 100

Expression
4 Core Practical- Immunology and 20PBCC31P 6 3 6 40 60 100
4 Advanced
Biochemistry lab

5 NMEC NME - Clinical 20PBCN31 5 4 3 40 60 100

Biochemistry
(Basics)

6 Online Course Practice for 20POLG31 1 1 - 100 - 100

SET/NET
Preparation -

General Total 30 23 600

NMEC: Non Major Elective Course

25 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc.
BIOCHEMISTRY -
SEMESTER IV

S.No. Components Title of the Course Hours Credits Exam. Marks

Course
Code per Hours
Int. Ext. Total
Week

1 Core Course- Environmental 20PBCC41N 6 5 3 40 60 100

10 Biochemistry

2 Core Course- Clinical 20PBCC42N 6 5 3 40 60 100

11 Biochemistry

3 Core Course- Developmental 20PBCC43N 6 5 3 40 60 100

12 Biology and
Genetics

4 Project Project - 22PBCC41PR 12 8 6 40 60 100

Research
Methodology
& Ethics
Total 30 23 400

26 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester I Hours/Week: 6
Core Course-1 CHEMISTRY OF Credits: 5
Course Code BIOPOLYMERS Internal External
20PBCC11 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: describe the composition, classification of biomolecules and its essential role
in the biological system. [K2]
CO2: sketch the structure of biomolecules and its properties. [K3]
CO3: identify the mechanism of action of biomolecules along with their structural
relationship. [K3]
CO4: evaluate the methods involved in the isolation and purification of
macromolecules. [K4]
CO5: compile the structural difference in biomolecules and techniques involved in
biomolecules structure identification. [K5]

UNIT I
Introduction to Biomolecules: Chemistry of monomeric units, polysaccharides, proteins and
nucleic acids [structure and function only]. Isolation and purification of polysaccharides, proteins
and nucleic acids. Determination of molecular weight and shape of polysaccharides, proteins and
nucleic acids. Protein- nucleic acid interaction and protein- other biological polymers interaction.

(15 Hours)

27 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II

Structural Investigation of Polysaccharides: General methods of investigating the structure-

methylation, acetylation, degradation by acid hydrolysis, enzymatic hydrolysis and per- iodate
oxidation methods. Occurrence, structure, isolation, properties, purification and biological functions
of glycans. A brief account of chitin, fructans, mannans, xylans, arabinans, galactans and
galacturonans. Occurrence, structure, isolation, purification, properties and biological functions
of mucopolysaccharides, bacterial cell wall polysaccharides, sialic acids and blood group
substances. A brief account of polysaccharides with a xylose backbone, polysaccharides with
glucose and mannose backbone. (20 Hours)

UNIT III

Properties of Proteins: Terminology- peptide bond, peptide, polypeptide and protein, functions
of protein. Structures- levels of structures of protein [primary structure, secondary, tertiary and
quaternary] conformation of protein structure, their analysis and forces, molecular modeling.
Properties of proteins in aqueous solutions: Isoelectric pH, acid base properties, electrophoretic
mobility, influence of ionic concentration on the protein solubility, hydrolysis of proteins,
denaturation and renaturation of proteins . (20 Hours)

UNIT IV

Metalloprotein: Metalloenzymes- Carboxypeptidase, Superoxide dismutase, Signal transduction

protein: Calmodulin, Oxygen carriers: Cytochrome & Hemoglobin, Structural Protein-Occurrence
& Structure of Keratin, Collagen. (15 Hours)

UNIT V

Nucleic acid Analysis: Structure of nucleic acid, chemical and enzymatic methods of sequence
analysis, properties of DNA in aqueous solution- sedimentation behavior, viscosity, hyperchromic
effect, melting point of DNA and hydrolysis of nucleic acids, Hybridization techniques and
chemical synthesis of nucleic acids. (20 Hours)

28 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
TEXT BOOKS

1. West E. S. & Todd W. R. (1974), Text book of Biochemistry, 4th Edition. New
York: MacMillan Publishing Company.
2. Lehninger. (2012), Principles of Biochemistry, 5thEdition, W H Freeman & Co.

REFERENCE BOOKS

1. Nathan Sharon. (1978). Complex carbohydrates, 2ndEdition.Kluwer Academic

Publishers, Dordrecht.

2. Charles R. Cantor, Paul R. Schimmel. (1989) ,Biophysical Chemistry, San Francisco: W.H.
Freeman and Company.

3. Geoffrey L.Zubay. (1998). Biochemistry, 4th edition, USA: Wm.C. Brown Publishers.
4. Ward Pigmann. (1977). The carbohydrates, 2nd Edition, Academic Press.
5. Ronald Voet& Judith G.Voet. (2006). Biochemistry, 2nd Edition. U.S.A: John Wiley & Sons.
6. Adams. (1986). Biochemistry of the Nucleic acid–, 10th Edition. Springer.

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCC11 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H M H M H H M - -
CO2 H H M M L H M H - -
CO3 H H H M L H H M L H
CO4 H H M H M H M M H L
CO5 H H M H M H M M H H

Dr.P.Annapoorani Dr.R.Salini
Head of the Department Course Designer

29 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester I Hours/Week: 6
Core Course-2 Credits: 5
PRINCIPLES OF BIOCHEMICAL AND
Course Code Internal External
BIOPHYSICAL TECHNIQUES
20PBCC12 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand the Biophysical, Biochemical and Molecular Biology
techniques. [K2]
CO2: apply the separation procedures such as Centrifugation, Chromatography
and Eletrophoretic techniques in biological investigations. [K3]

CO3: determine the Biochemical and Biophysical characterization of macromolecules and their
complexes for structural biology experiments. [K3]
CO4: analyze the practical and data handling skills required to undertake the Bio Technical
research. [K4]
CO5: evaluate the principles and techniques of Biochemistry that motivates the students for
higher education, acquiring skills in separation techniques to identify different
biomolecules, undertaking research, and for becoming health professionals. [K5]
UNIT I
Stoichiometry: Normality, Molarity, Molality and Milliosmoles. Ionic strength. pH. pOH, Henderson-
Hesselbach equation, Buffers, pH of body fluids. Measurement of pH by indicators, Zwitterions. pH
dependent ionization of amino acids and proteins. Colloids and their applications, Viscosity, Surface
tension and Donnan membrane equilibrium. Principles of electrochemical techniques-measurement of pH
by glass electrode and hydrogen electrode Oxygen electrode-principles, operation of a Clarke electrode
and its applications.
(20 Hours)

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 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II

Electrophoresis: Electrophoresis – Principle, types- Agarose gel electrophoresis, SDS – Polyacrylamide

Gel Electrophoresis, 2D-Polyacrylamide Gel Electrophoresis, Immunoeletrophoresis. Visualizing protein
bands-CBB and Silver Staining. MS-MALDI-TOF. Introduction to proteomics.
(15 Hours)
UNIT III
Chromatography & Centrifugation: Chromatography-Principle, Procedure and Applications of GLC,
HPLC, FPLC Cell Fractionation Techniques- Cell lysis, Differential and density gradient centrifugation.
Ultracentrifugation-Preparative and Analytical Ultracentrifuge.
(15 Hours)

UNIT IV
Spectroscopy: Spectroscopy- X-ray crystallography, X-ray fiber diffraction, X-ray scattering. NMR,
ESR, Spectro fluorimetry, Flame photometry, UV Spectroscopy, FTIR, Biochips (DNA chips, Protein
chips, Sensor chips) Atomic Absorption Spectroscopy, and Raman Spectroscopy
(20 Hours)
UNIT V

Molecular Biology Techniques: Isolation of Nucleic acids, Restriction endonucleases, Restriction

mapping, Nucleic acid probes, Oligonucleotide probes and labelling of nucleic acid probes. Nucleic acid
hybridization- Southern, Northern, Western hybridization, Restriction fragment length polymorphism.
DNA finger printing, Polymerase chain reaction. (20 Hours)

TEXT BOOK
1. V.Kumaresan (2012), Biotechnology , 6th edition, Saras Publication
REFERENCE BOOKS
1. Keith Wilson & John Walker (1995), Practical Biochemistry, Principles & Techniques,

4th Edition, Cambridge University Press.

2. VinashUpadhay, Kakoli Upadhyay and Nirmalendu Nath (2002), BioPhysical Biochemistry,
Principles & Techniques, 3rd Edition, Himalaya Pub. House, Mumbai.
3. Boyer.R (2000), Modern Experimental Biochemistry.3rdedition. Addison-Wesley Pub.
Co. University of Michigan.

31 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

Course PO1 PO2 PO3 PO4 PO 5 PO6 PO7 PO8

Code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCC12 1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H L M M M - M M -
CO2 H H H H H H - M M -
CO3 H H H H H H M M M -
CO4 H H M H H H M M M M
CO5 H H H M H H M M M M

Dr.R.Mallika
Mrs. M. Rajakumari
Dr.P.Annapoorani Course Designers
Head of the Department

32 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -21 onwards)

Semester I Hours/Week: 6
Core Course-3 BIOCHEMICAL AND ENVIRONMENTAL Credits: 5
Course Code TOXICOLOGY Internal External
20PBCC13 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: describe the terms and basic principles of toxicology, mechanism of toxic effects of
toxicants and factors affecting disposition of toxicants. [K2]
CO2: identify health conditions linked to selected toxic exposures from food, lifestyle,
environment, workplace and home. [K3]
CO3: apply pharmacokinetic and pharmacodynamic principles that impact administration,
ADME, efficacy, potency, effectiveness and biological activity of drugs and
toxins. [K3]
CO4: analyze the types of toxicology, toxicants, metals and its disposition, responses in
target organ, non-organ directed toxicity. [K4]
CO5: assess the techniques and methods of toxicity and fate of toxicants in humans. [K5]

UNIT I
General principles of Toxicology -Definition, Toxicologic terms and definitions- Toxin, Toxicant,
Toxicity, Hazard, Risk, Acute exposure, Chronic exposure, Synergism, Additive effect, Potentiation
effect, Antagonism. classification of toxicology, Classification of toxic agents. Desired and undesired
effects. Principles of selective toxicity: cooperative morphology, comparative biochemistry, comparative
cytology. Toxicity assessment: acute, subchronic, chronic exposure, determination of ED50 and LD50
values, tests for mutagencity, carcinogenicity, genotoxicity, Ames test. (20 Hours)

33 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II

Disposition of Toxicants: Factors affecting disposition of toxicants: absorption, distribution,

biotransformation, elimination. Absorption through gastro-intestinal tract, lungs, skin.
Distribution: Storage in tissues, blood-brain barrier, passage across placenta, redistribution.
Biotransformation, Phase I and II reactions, metabolic interrelationship, antidotal therapy.
Excretion: urinary, fecal, exhalation, other routes. Toxicokinetics: classical and physiological
kinetics. (15 Hours)

UNIT III

Non- organ directed toxicity: Chemical carcinogenesis: definition, mechanisms. Genetic

toxicology: definition, health impacts and mechanism. New approaches for genetic toxicology,
advances in cytogenetics. (15 Hours)

UNIT IV

Target organ toxicity: Skin: skin as a barrier, dermatitis, acne, urticaria. Toxic responses of the
blood: blood as a target organ, toxicology of erythron, leucon and platelets. Toxic responses of
the liver: physiology and pathophysiology, factors in liver injury, mechanism of liver injury.
Toxic responses of the respiratory systems: lungs structure and functions, pulmonotoxic
agents, pathogenesis of chemical induced damage, acute and chronic responses of lungs to
injury. (20 Hours)

UNIT V

Applications of Toxicology:Food toxicology: Role of diet in cardiovascular diseases and cancer.

Toxicology of food additives. Metal toxicity: Toxicology of arsenic, mercury, lead, and cadmium.
Environmental factors affecting metal toxicity – effect of light, temperature and pH. Occupational
toxicology - Industrial effluent toxicology & environmental health. (20 Hours)

TEXT BOOKS

1. Casarette, Doull and Klaassen (1992), Toxicology,8th edition, McGraw-

Hill,New York.
2. Ernest Hodgson (2004) A Textbook Of Modern Toxicology.3rd edition, A John Wiley
& sons, inc., Publication, USA

34 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS

1. Marrs and Turner (1995), General and applied toxicology, Macmillan Press Ltd.
2. Williams RT (1947), Detoxification mechanisms, J.Wiley & Sons, New York.
3. Albert A.( 1979), Selective Toxicity ,Springer Link

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCC13
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H M H M M H M L - -
CO2 H H H H M H H L M H
CO3 H H M H M H H M M H
CO4 H H H H M H H M M H
CO5 H H H H M H H m H H

Dr.P.Annapoorani Mrs.K.Sudha Rameshwari

Head of the Department Course Designer

35 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -21onwards)
Semester: I Hours/Week: 6
Core Practical-1 BIOCHEMICAL TECHNIQUES AND Credits: 3
Course Code ANALYSIS LAB Internal External
20PBCC11P 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: apply the principles and methodologies of partial enzyme purification,
spectrophotometry and chromatographic techniques in biomolecules
analysis.[K3]
CO2: sketch flowcharts for the partial enzyme purification, spectrophotometry and
Chromatographic techniques of various bioanalytes. [K3]
CO3: observe and calculate the results for the spectrophotometry, chromatographic
techniques of biological samples and complete the record work. [K3]
CO4: infer the normal and abnormal parameters of biological samples
analyzed by spectrophotometry, chromatographic techniques. [K4]
CO5: evaluate the outcomes of the modifications in sample
analysis using spectrophotometry, chromatographic
Techniques. [K5]
Experiments
1. Buffer preparation, pka value

2. Spectrometric estimation

(i) Estimation of Riboflavin

(ii) Estimation of Thiamine

36 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

(iii) Estimation of Calcium

(iv) Estimation of amino acid by formal titration

(v) Estimation of iron by Wong‟s method.

3. Secondary metabolites analysis

(i) Phenol
(ii) Flavonoids
4. Lipid separation by TLC
5. Serum aminoacids separation by paper chromatography
6. Partial purification of enzymes by column chromatography : Amylase /urease/alkaline
Phosphatase. Enzyme kinetics: determination of Km and Vmax
REFERENCE BOOKS
1. Palanivelu P.(2004) Analytical Biochemistry & Separation Techniques ,Palkalai Nagar,
Madurai:4/e, 21st Century Publication.

2. Harold Varley (1980), Practical Clinical chemistry-Vol.1&II ,5thedition.New York: Inter

Science Publishers, Inc.

2. Sadasivam .S& A. Manickam (1996), Biochemical Methods, 2nd edition. New Delhi:
India NewAge International Publishers.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCC11P PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 M M M M M M M M - M
CO2 M M M M M M M M - M
CO3 H H H H H M H L - H
CO4 H H H H H H H M - H
CO5 H H H H H H H M L H

Dr.P.Annapoorani Mrs. R.Gloria Jemmi Christobel

Head of the Department Course Designer

37 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester I Hours/Week: 6
DSEC-1 CELLULAR BIOCHEMISTRY AND Credits: 5
Course Code VIROLOGY Internal External
20PBCE11 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: comprehend the chemical composition of cell membrane, cellular processes on the
plasma membrane, regulated and unregulated events inside and outside the cell.
[K2]
CO2: identify the occurrence of cellular mechanisms at molecular level based on ionic
transport, ATP synthesis, cell signaling and cell division process. [K3]
CO3: apply the knowledge about plasma membrane on various cellular events & infer
the link between the life cycle of viruses and oncogenesis mechanism. [K3]
CO4: dissect a cellular problem into its key features by thinking in an integrated
manner and examining the problems from a different perspective. [K4]
CO5: interpret the cell biological principles based on sound scientific principles and correlating
them with the modern cell biology research. [K5]
UNIT I
Molecular organization: Freeze fracture and fluid mosaic model. Composition: membrane lipids,
membrane protein, membrane fluidity and molecular mobility of lipids and proteins. Model
membrane isolation: Techniques of making multilamellar vesicles, bilayer reconstitution of
proteins into vesicles, liposomes. Isolation and characterization of plasma membrane.
Membrane fusion in exocytosis and endocytosis. (20 Hours)

38 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II

Electron Transport Chain: Structure of mitochondria, respiratory chain - Enzyme complexes,

inhibitors and uncouplers of respiratory chain, energy transfer, oxidative phosphorylation, proton
gradient and ATP synthesis- F1 ATPase. (13 Hours)

UNIT III

Membrane transport: Small molecules- simple diffusion, Facilitated diffusion- pores, channels,
properties. Carriers- specific ionophores. Active transport- Na pump, Ca2+ pump. Secondary
active transport-Na dependent active transport. (12 Hours)

UNIT IV

Cell junctions: Desmosomes, tight junction, gap junctions. Extracellular matrix- Collagen,
chemistry and assembly. Organization and role in cell adhesion- proteoglycans and
glycosaminoglycans, elastin. Molecular aspects of cell division- Cell cycle. Membrane receptors,
communications: chemical signaling between cells- hormones and neurotransmitters. Signal
transduction: cAMP, G protein, IP. Structure and organization of the nervous system.
(27 Hours)
UNIT V

Bacteriophages & Cancer Biology: Tumor viruses: DNA virus- SV40 replication, RNA viruses
- RSV replication. Cancer Biology- cell cultures, cell lines, and cell transformation. Chemical
differences between normal and cancer cells, surface changes in cancer cells. Agents that cause
cancer in animals. Chemical carcinogens and radiation. Oncogenesis mechanism. MTT assay.
Comet assay. (18 Hours)

TEXT BOOKS
1. Powar C.B. (1992), General Microbiology, Vol–II ,2nd Edition, Himalaya Pub.
House, Mumbai.
2. Gerald Karp (1999), Cell & Molecular Biology, John Wiley &sons, Newyork.
3. Becker, Kleinsmith, Hardin (2002). The world of the cell, 4th Edition, Benjamin
Cummings.

39 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS

1. Ronald Voet & Judith G.Voet (2006), Biochemistry, 2nd Edition, John Wiley & Sons,
U.S.A.
2. Benjamin Lewin (2004), Genes VIII, Oxford University Press, New York.
3. S.C.Rastogi (1986), Cell and Molecular biology, 3rd edition, New Age international Pvt
Ltd, NewDelhi.
4. De Robertis (2005), Cell and Molecular Biology, 8th Edition, B.I.Waverly Pvt Ltd,
New Delhi.

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCE11
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 M M M L L - H - - L

CO2 M L L M M M H M - L

CO3 H H H H H M H M - -

CO4 H H H H H H H H L H

CO5 H H H H H H H H L H

Dr.P.Annapoorani Mrs. R.Gloria Jemmi Christobel

Head of the Department Course Designer

40 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester I Hours/Week: 6
DSEC-1 ENZYMES AND ENZYME Credits: 5
Course Code TECHNOLOGY Internal External
20PBCE12 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: describe the structure, functions, mechanism of action of enzymes, kinetics of
enzyme catalyzed reactions, enzyme inhibitions, regulatory process and their
application in commercial production. [K2]
CO2: apply their knowledge in the commercial synthesis of novel products
using the enzyme kinetics in living systems. [K3]
CO3: compare and contrast the uses of enzyme technology with current
applications in a diverse range of industries. [K3]
CO4: evaluate the function of cofactors in enzyme catalyzed reactions,
immobilization of enzymes, exposure of wide applications of enzymes and
future potential. [K4]
CO5: detect the immobilization of enzymes, commercial enzyme production and the
presence of aminoacids in the active sites. [K5]
UNIT I
Introduction: Active site-definition, salient features, list of functional groups, Identification of
amino acids present in the active site by chemical modification, affinity labeling and site directed
mutagenesis. Specificity of enzymes. Cofactor as carriers: Redox carriers (NAD/NADP,
flavoprotein, lipoate, glutathione, ascorbic acid, quinines, cytochromes). Carriers of one
carbon group - (Tetrahydrofolate, Homocysteine, Biotin), Phosphate carrier (ATP), Glycosyl
carrier (UTP). (15 Hours)

41 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II

Enzyme Kinetics: Concept of ES complex, derivation of Michaelis-Menten equation for uni-

substrate reactions. Different plots for the determination of Km &Vmax and their physiological
significance. Importance of Kcat/Km. Enzyme inhibition: Irreversible inhibition and reversible-
Competitive, non- competitive, uncompetitive. Reactions of two substrates. (20 Hours)

UNIT III

Enzyme Regulation: Allosteric enzyme –definition, classification and properties. Allosteric

regulation - Concerted model of Monod et al., and sequential model of Koshland et al.,
allosteric and feedback inhibition. NAD/NADH ratio, adenylate charge. (15 Hours)

UNIT IV

Mechanism Enzyme action: Activation energy, Fischer‟s Lock and Key hypothesis and
Koshland‟s induced fit model. Proximity and orientation effect, Strain & distortion theory. Acid –
base catalysis - Ribonuclease, Lysozyme. Covalent catalysis – Chymotrysin, Carboxy peptidase.
Multienzyme complex.
(25 Hours)

UNIT V

Enzyme technology: Immobilized enzyme: Techniques of immobilization and its

applications. Commercial production of enzymes – amylases, cellulases, pectinases, protease
and applications of enzymes in industries. Enzyme as Biosensor - Calorimetric biosensor and
Immunosensor. Enzyme engineering, artificial enzymes in organic solvents.
(15 Hours)

42 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
TEXT BOOKS
1. Ronald Voet & Judith G.Voet (2006), Biochemistry, 2nd Edition, John Wiley &
Sons, U.S.A
2. J.L.Jain, Nitin Jain and Sunhay Jain (2013), Fundamentals of Biochemistry, 6th
edition, S.Chand publishing.

3. Dr.P.Asokan (2003), Enzymes, 1st edition, Chinnaa Publications.

4. R.C.Dubey and D.K.Maheswari (2013), Text book of Microbiology,4thedition,S Chand
Publishing.

REFERENCE BOOKS

1. Pandey, A. Webb, C., Soccol C.R. and Larroche, C. (2004).Enzyme Technology,

Published by Asia Tech Publishers INC. New Delhi.
2. Dixon and Web (1979), Enzymes, 3 rd Edition , Academic Press, New York
3. Geoffrey L.Zubay (1998), Biochemistry, 4th edition, Wm.C. Brown Publishers, USA
4. Trevor Palmer (2001), Enzymes: Biochemistry, Biotechnology and Clinical
Chemistry, Horwood.

5. Martin Chaplin and Christopher Bucke (1990). Enzyme Technology, Cambridge University
Press.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCE12
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H M M M H M - - -
CO2 H H M M M M M - - M
CO3 H M M M M M M - - -
CO4 H H M M M H H - - M
CO5 H H M M M M H - - M

Dr.P.Annapoorani K.Sudha Rameshwari

Head of the Department Course Designer

43 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester I Hours/Week: 6
DSEC-1 Credits: 5
Course Code DAIRY BIOCHEMISTRY Internal External
20PBCE13 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand the composition and physicochemical characteristics of the main components
of milk. [K2]
CO2: apply the knowledge of chemistry of dairy components and find the impacts of processing
conditions on milk and dairy products. [K3]
CO3: identify the dairy products manufacturing and key functions of the processing steps
involved. [K3]
CO4: analyze the food adulteration and contamination of food, food laws and standards. [K4]
CO5: design methods of dairy production, and refrigeration and storage techniques. [K5]
Unit I
Composition of Milk, Food and Nutritive Value of Milk, Classification, Colostrums and its
properties and difference from normal milk, Correlations amongst Compositional parameters,
Legal standards of milk, Chemical test.
(20 Hours)
UNIT II
Reception & Treatment of Milk at the Dairy Plant: Reception, Chilling, Clarification and
Storage Coagulation and heat stabilizing milk.
(15 Hours)

UNIT III
Homogenization – Definition, Effect of Homogenization on Physical properties of Milk.
Elementary knowledge about indigenous and modern dairy products.
(15 Hours)

44 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT IV

Thermal Processing of Milk; Definition & Description of Processes – Pasteurization,

Thermization, Sterilization and UHT Processing. Collection and Transportation of Milk,
Preservation at Farm, Refrigeration. (20 Hours)

UNITV

Adulteration in milk & their detection, Defects in Market Milk, Standardized Milk,
Manufacturing of Special Milk – Toned, Doubled Toned, Reconstituted, Recombined, Flavored
Milk (20 Hours)

TEXT BOOKS

1. Dubey R.C, (2000). Text book on Microbiology,1st edition. S.Chand & Co., New Delhi.

2 .John Wiley and sons , Dairy Chemistry and Physics. New York,

REFERENCE BOOKS

1. S.K.DEY, Outlines of Dairy Technology, Oxford IBH Pub. NDRI Market milk.
2. Pelczar, M.J., Chan,E.C.S and Kreig,N.R (1993).Microbiology,5th Edition, Tata Publishing
Co., Ltd., New Delhi.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCE13 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H M M M M M M M M

CO2 H H M H H M M M - -

CO3 H M H M M H M - - M

CO4 H H M H H M H M M -

CO5 H H H H H H H - - -

Dr.P.Annapoorani M.Rajakumari
Head of the Department Course Designer

45 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester II Hours/Week: 5
Core Course-4 ENDOCRINOLOGY & METABOLIC Credits: 4
Course Code REGULATION Internal External
20PBCC21 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: demonstrate various metabolic pathways of various biomolecules, hormones of endocrine
glands and their mechanism of action. [K2]
CO2: apply the crucial role of hormones with regard to the integration of metabolic
Pathways. [K3]
CO3: find the integrated approach of anabolic and catabolic pathways of various biomolecules
mediated by hormones. [K3]
CO4: analyze the role of hormones and key enzymes that regulate various metabolic
Pathways. [K4]
CO5: assess the involvement of hormones and organs in the metabolic pathways of the living
System. [K5]
UNIT I
Introduction to Hormones: Characteristics of hormone system, functions & mechanism of action of
hormones. Growth factors: somatomedin & erythropoietin, Nerve growth factor, epidermal growth
factor, fibroblast growth factor, GI tract hormones. Chemistry, biosynthesis, secretion, biochemical
actions of pituitary, thyroid, parathyroid, adrenal & gonadal hormones. (15 Hours)

46 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT II
Hormonal regulation of carbohydrate metabolism: Glycolysis & gluconeogenesis:
Phosphofructokinase as the key enzyme in glycolysis; role of fructose 2, 6 diphosphate in liver &
muscle; hexokinase and pyruvate kinase as regulatory enzymes in glycolysis, pyruvate dehydrogenase
complex & its regulation. Reciprocal of glycolysis & gluconeogenesis (15 Hours)

UNIT III
Glycogen metabolism: Glycogen as an efficient storage form of glucose, cAMP& coordinated control
of Glycogenesis & Glycogenolysis. Phosphorylase activation & inactivation. Effect of phosphorylation
of synthase. Action of phosphatases. HMP shunt - glucose – 6- phosphate dehydrogenase as a
regulatory enzyme – role of NADPH in metabolism. TCA cycle, Citrate synthase, Isocitrate
dehydrogenases & α- Ketoglutarate dehydrogenase as regulatory site in TCA cycle. Transport of
NADH into mitochondria. (15 Hours)

UNIT IV
Fatty acid and Amino acid Metabolism: Control of Acetyl-coA carboxylase, role of hormones,
effect of diet on fatty acid synthesis. Regulation of biosynthesis of Triacylglycerol, cholesterol,
phosphatidyl ethanolamine sphingomyelin. Metabolism of triacylglycerol during stress. Fatty acid
oxidation, role of carnitine control of oxidation, regulation of ketogenesis, Metabolism of
aromatic amino acids, prostaglandins & thromboxanes. (15 Hours)

UNIT V
Urea Cycle & Nucleic Acid Metabolism: Regulation of glutamate dehydrogenase & Urea
cycle. Regulation of purine & pyrimidine nucleotide biosynthesis. Integration of metabolism: Key
junction in metabolism: Glucose- 6- PO4, Pyruvate & Acetyl coA. Metabolic profiles of major
organs – Brain, muscle, liver and adipose tissue. (15 Hours)
TEXT BOOKS

1. Stryer L (2003). Biochemistry 2nd Edition, W.H. Freeman and Company, NY.
2. Chatterjee (2011), Text book of Medical Biochemistry, 8th edition, Jaypee Brothers Medical
(P) Ltd. New Delhi.

3. Lehninger A.L. (2008). Principles of Biochemistry , 5th edition, Palgrave Macmillan UK.

47 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
REFERENCE BOOKS
1. Ronald Voet& Judith G.Voet (2006), Biochemistry, 2nd Edition, John Wiley & Sons, U.S.A
2. Geoffrey L.Zubay (1998), Biochemistry, 4th edition, Wm.C. Brown Publishers, USA.
3. Conn E.E., Stumpf P.K ,Bruening G (1987). Outlines of Biochemistry 5/e –R.H, John Wiley
& Sons, USA.

4. Voet D & Voet J.G (1990). Biochemistry, John Wiley & Sons, NY.

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCC21
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 M M M L L L M - - -

CO2 H H L M M M M - - H

CO3 H H M H H M H M - H

CO4 H H H H H H H M L H

CO5 H H H H H H H H L H

Dr.P.Annapoorani Mrs. R.Gloria Jemmi Christobel

Head of the Department Course Designer

48 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)
Semester II Hours/Week: 5
Core Course-4 ENDOCRINOLOGY & METABOLIC Credits: 4
Course Code REGULATION Internal External
20PBCC21N 40 60

COURSE
OUTCOMES
On completion of the course, the students will be able to
CO1: demonstrate various metabolic pathways of various biomolecules, hormones of endocrine
glands and their mechanism of action [K2]
CO2: apply the crucial role of hormones with regard to the integration of metabolic Pathways [K3]
CO3: find the integrated approach of anabolic and catabolic pathways of various biomolecules
mediated by hormones. [K3]
CO4: analyze the role of hormones and key enzymes that regulate various metabolic
pathways [K4]
CO5: assess the involvement of hormones and organs in the metabolic pathways of the living
system [K5]
UNIT I
Introduction to Hormones: Characteristics of Hormone System, Functions & Mechanism of Action of
Hormones. Growth Factors: Somatomedin & Erythropoietin, Nerve Growth Factor, Epidermal Growth
Factor, Fibroblast Growth Factor, GI Tract Hormones. Chemistry, Biosynthesis, Secretion, Biochemical
Actions of Pituitary, Thyroid, Parathyroid, Adrenal & Gonadal Hormones (15 Hours)

UNIT II
Hormonal Regulation of Carbohydrate Metabolism: Glycolysis & Gluconeogenesis:
Phosphofructokinase As The Key Enzyme in Glycolysis; Role of Fructose 2, 6 Diphosphate in Liver &
Muscle; Hexokinase and Pyruvate Kinase as Regulatory Enzymes in Glycolysis, Pyruvate
Dehydrogenase Complex & its Regulation. Reciprocal of Glycolysis & Gluconeogenesis. (15 Hours)

49 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT III

Glycogen metabolism: Glycogen as an efficient storage form of glucose, cAMP& coordinated control of
Glycogenesis & Glycogenolysis. Phosphorylase activation & inactivation. Effect of phosphorylation of
synthase. Action of Phosphatases. HMP shunt-Glucose-6-phosphate dehydrogenase as a Regulatory
enzyme, Role of NADPH in metabolism. TCA cycle, Citrate synthase, Isocitrate dehydrogenases & α-
Ketoglutarate dehydrogenase as Regulatory site in TCA cycle. Transport of NADH into mitochondria.
Heme metabolism : Biosynthesis, catabolism (porphyrins and bilirubin metabolism) (15 Hours)
UNIT IV
Fatty acid and Amino acid Metabolism: Control of Acetyl-Coa Carboxylase, Role of Hormones, Effect
of Diet On Fatty Acid Synthesis. Biosynthesis of Triacyl Glycerol, Cholesterol, Phosphatidyl
Ethanolamine & Sphingomyelin. Metabolism of Triacylglycerol during Stress. Fatty Acid Oxidation,
Role of Carnitine Control of Oxidation, Regulation of Ketogenesis, Metabolism of Aromatic Amino
Acids, Prostaglandins & Thrombaxanes. (15 Hours)
UNIT V
Urea Cycle & Nucleic Acid Metabolism: Regulation of Glutamate Dehydrogenase & Urea Cycle.
Regulation of Purine & Pyrimidine Nucleotide Biosynthesis. Integration of Metabolism: Key Junction in
Metabolism: Glucose- 6- PO4, Pyruvate & Acetyl CoA. Metabolic Profiles of Major Organs - Brain,
Muscle, Liver and Adipose Tissue. (15 Hours)
TEXT BOOKS
1. Stryer L (2003). Biochemistry 2nd Edition, W.H. Freeman and Company, NY.
2. Chatterjee (2011), Text book of Medical Biochemistry, 8th edition, Jaypee Brothers. New Delhi.
3. Lehninger A.L. (2008). Principles of Biochemistry , 5th edition, Palgrave Macmillan UK.
REFERENCE BOOKS
1. Ronald Voet& Judith G.Voet (2006), Biochemistry, 2nd Edition, John Wiley & Sons, U.S.A
2. Geoffrey L.Zubay (1998), Biochemistry, 4th edition, Wm.C. Brown Publishers, USA.
3. Conn E.E., Stumpf P.K ,Bruening G (1987). Outlines of Biochemistry 5/e –R.H, John Wiley
& Sons, USA.
4. Voet D &Voet J.G (1990). Biochemistry, John Wiley & Sons, NY.

50 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

Course Code
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
(20PBCC21N)
1a 1b 2 3a 3b 4 5 6 7 8

CO 1 H M M M M M H M M M
CO 2 H M M H H H H M M M
CO 3 M M H H M H H M M M
CO 4 M M H H M H H M M M
CO 5 M M H H M H H M M M

Dr.P.Annapoorani Dr. R.Gloria Jemmi Christobel

Head of the Department Course Designer

51 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester II Hours/Week: 5
MICROBIAL BIOCHEMISTRY
Core Course-5 Credits: 4
AND FERMENTATION
Course Code TECHNOLOGY Internal External
20PBCC22 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand how microbes are relevant in technological developments for
industries related to food and fermentation and their various, metabolic energy
yielding pathways. [K2]
CO2: develop knowledge about microbial metabolism, growth, energy generation, various
fermentation pathways, energetic its role in science and industry today. [K3]
CO3: apply the knowledge of microbial biochemistry and fermentation technology
Including areas such as energy metabolism, microbial biosynthesis and
industrial production, purification of compounds and their application to
research, development and societal needs. [K3]
CO4: analyze different fermentation techniques, bioreactor design, inoculum
development for industrial fermentation, the use of microorganisms for the
value-added products through fermentation processes, the production of
secondary metabolites. [K4]
CO5: assess fermentation technology and requirements; production of commercially
important microbial products and various metabolic activities in
microorganisms. [K5]

52 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT I

Metabolic and energy yielding reactions of sugars: Transport of sugars into the bacterial cell-
The phosphotransferase system, Transport of non-PTS sugars. Membrane bound transport systems
– E.coli lactose permease, ß-methyl galactoside system. Pathways of glucose degradation: EMP,
HMP, ED pathways, and Phosphoketolase pathways. Aerobic pathways of pyruvate
metabolism-TCA cycle, electron transport and glyoxylate cycle, anaplerosis. Utilization of
one carbon and two carbon compounds-Glycerate pathway and serine pathway. Interrelationship
of EMP, HMP and ED pathways.
(15 Hours)
UNIT II
Metabolism of lipids: Oxidation of fatty acids in microbes, Oxidation of fatty acids with odd
number of carbon atoms, oxidation of branched chain fatty acids, Oxidation of aliphatic and
aromatic hydrocarbons. Biosynthesis of straight chain , branched chain fatty acids, biosynthesis of
superchain fatty acids, glycerol dissimilation, synthesis of triglycerides, phospholipids and
glycolipids. Catabolism of phospholipids, poly isoprenoid biosynthesis.
(15 Hours)
UNIT III
Microbial Biosynthesis: Synthesis of storage polymers- Poly Beta hydroxybutyrate and poly
phosphate. Secondary metabolites - Biosynthesis of patulin as an example. Extracellular enzymes.
Photosynthesis: Photosynthetic structures, types of bacterial photosynthesis, photosynthetic
pigments, photosynthetic electron transport, photophosphorylation. Co2 fixation - Calvin cycle and
reductive carboxylic acid cycle. Distinction between prokaryotic and eukaryotic photosynthesis.
(15 Hours)
UNIT IV
Fermentation technology: Fermentation - definition, Types of fermentation- Homolactic and
hetero lactic fermentation, propionic fermentation and formic acid fermentation. Surface,
submerged fermentation and solid state fermentation. Design and operation of Fermentor -
Basic concepts for selection of a reactor, Packed bed reactor, Fluidized bed reactor, Trickle bed
reactor, Bubble column reactor. Downstream processing-precipitation, Centrifugation,
filtration, solvent extraction, chromatographic purification and affinity purification.
(15 Hours)

53 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UN
IT
V

Industrial Biotechnology: General techniques of the inocula buildup for industrial

fermentations. Scale up and scale down fermentation. Cheap raw materials as substrates.
Industrial production- Streptomycin, Penicillin (antibiotic), Beer, Wine (alcohol), organic
acid (Citric acid, Lactic acid), L-glutamic acid (amino acid), amylase, Protease
(enzymes), Vitamins (B12.Production of
single cell proteins, production of fermented
foods.
(15 Hours)

TEXT BOOKS

1. C.B.Power (1992), General Microbiology, Vol I, 8th Edition, Himalaya

Publishing house, Mumbai.
2. R.C.Dubey and D.K.Maheswari (2013), Text book of Microbiology,4thedition, S Chand
Publishing.
3. L.E.Casida, JR (1994). Industrial Microbiology, New Age International Publ.
4. Patel A.H.(1999 ), Industrial microbiology , Macillan India Ltd.
REFERENCE BOOKS

1. Caldwell D.R(2002.). Microbial physiology and metabolism, William C Brown

Publishers, USA.
2. Stanbury O.F., Whitakar A., & Hall S.J.(1997), Principles of Fermentation
Technology, Aditya Books (P) Ltd., New Delhi.
3. Schlegel H.G. (1995),General Microbiology, 7th edition, Cambridge University
Press, Cambridge
4. Puvanakrishnan.R, Sivasubramanian.S, Hemalatha.T.(2012).Microbial
technology concepts and applications.MJP publishers, New Delhi.

54 17th Academic Council Meeting 31.01.2023

 PO1 PO2 PO3 PO4 Curriculum
PO5 for M.Sc.
PO6BIOCHEMISTRY
PO7 PO8
Course Code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCC22
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H M M H H M M - -
CO2 H H M M H H M H - -
CO3 H H H H H H H H M H
CO4 H H H H H H M H M H
CO5 H H M H H H M H M H

Dr.P.Annapoorani Mrs.K. Sudha Rameshwari

Head of the Department Course Designer

55 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)

Semester II Hours/Week: 5
Core Course-5 MICROBIAL BIOCHEMISTRY Credits: 4
AND FERMENTATION
Course Code TECHNOLOGY Internal External
20PBCC22N 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand how microbes are relevant in technological developments for industries
related to food and fermentation and their various, metabolic energy yielding
pathways. [K2]
CO2: develop knowledge about microbial metabolism, growth, energy generation,
various fermentation pathways, energetic its role in science and industry today. [K3]
CO3: apply the knowledge of microbial biochemistry and fermentation technology including
areas such as energy metabolism, microbial biosynthesis and industrial production,
purification of compounds and their application to research, development and societal
needs. [K3]
CO4: analyze different fermentation techniques, bioreactor design, inoculum
development for industrial fermentation, the use of microorganisms for the value-
added products through fermentation processes, the production of secondary
metabolites. [K4]
CO5: assess fermentation technology and requirements; production of commercially
important microbial products and various metabolic activities in microorganisms.
[K5]

56 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT I
Metabolic and energy yielding reactions of sugars: Transport of sugars into the bacterial cell-
The phosphotransferase system, Transport of non-PTS sugars. Membrane bound transport systems
– E.coli lactose permease, ß-methyl galactoside system. Pathways of glucose degradation:
EMP, HMP, ED pathways, and Phosphoketolase pathways. Aerobic pathways of pyruvate
metabolism-TCA cycle, electron transport and glyoxylate cycle, anaplerosis. Utilization of
one carbon and two carbon compounds-Glycerate pathway and serine pathway.
Interrelationship of EMP, HMP and ED pathways. (15 Hours)

UNIT II
Metabolism of lipids: Oxidation of fatty acids in microbes, Oxidation of fatty acids with odd
number of carbon atoms, oxidation of branched chain fatty acids, Oxidation of aliphatic and
aromatic hydrocarbons. Biosynthesis of straight chain , branched chain fatty acids, biosynthesis of
superchain fatty acids, glycerol dissimilation, synthesis of triglycerides, phospholipids and
glycolipids. Catabolism of phospholipids, poly isoprenoid biosynthesis.
(15 Hours)
UNIT III
Microbial Biosynthesis: Synthesis of storage polymers- Poly Beta hydroxybutyrate and poly
phosphate. Commercially important microbial polysaccharides and their applications.
Secondary metabolites - Biosynthesis of patulin as an example. Extracellular enzymes.
Photosynthesis: Photosynthetic structures, types of bacterial photosynthesis, photosynthetic
pigments, photosynthetic electron transport, photophosphorylation. Co2 fixation - Calvin
cycle and reductive carboxylic acid cycle. Distinction between prokaryotic and eukaryotic
photosynthesis. (15 Hours)
UNIT IV
Fermentation technology: Fermentation - definition, Types of fermentation- Homolactic and
hetero lactic fermentation, propionic fermentation and formic acid fermentation.
Surface, submerged fermentation and solid state fermentation. Design and operation of
Fermentor - Basic concepts for selection of a reactor, Packed bed reactor, Fluidized bed
reactor, Air lift bioreactor, Trickle bed reactor, Bubble column reactor. Downstream
processing-precipitation, Centrifugation, filtration, solvent extraction, chromatographic
purification and affinity purification. (15 Hours)

57 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT V
Industrial Biotechnology: General techniques of the inocula buildup for industrial fermentations.
Scale up and scale down fermentation. Cheap raw materials as substrates. Industrial
production-antibiotic (Streptomycin, Penicillin), alcohol (Beer, Wine), organic acid (Citric acid,
Lactic acid), aminoacid (L- glutamic acid), enzymes (amylase, Protease), Vitamins (B12).
Production of single cell proteins from wastes and wood. Production of fermented foods.
(15 Hours)
TEXT BOOKS
1. Caldwell D.R (2002.). Microbial physiology and metabolism, William C Brown Publishers, USA.
2. Joanne Willey, Kathleen Sandman and Dorothy Wood (2019). Prescott‟s Microbiology
3. U.Satyanaayana (2015), Biotechnology, Arunabha sen Books and allied (p) Ltd., Kolkata.
4. Lehninger A.L. (2008). Principles of Biochemistry , 5th edition, Palgrave Macmillan UK.
REFERENCE BOOKS
1. Schlegel H.G. (1995), General Microbiology, 7th edition, Cambridge University
Press, Cambridge
2. L.E.Casida, JR (1994). Industrial Microbiology, New Age International Publication
3. R.C.Dubey and D.K.Maheswari (2014), A text book of Biotechnology, 5th edition ,S Chand
Publishing.
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
Course Code
(20PBCC22N)
1a 1b 2 3a 3b 4 5 6 7 8

CO 1 H H M M H M M M M M

CO 2 H H M M H M M H M M

CO 3 H H H M H H H H M H

CO 4 H H M M H M M H M H

CO 5 H H M M H M M H M H

Dr.P.Annapoorani Mrs.K. Sudha Rameshwari

Head of the Department Course Designer

58 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester II Hours/Week: 5
Core Course- 6 MOLECULAR BIOLOGY AND GENETIC Credits: 4
Course Code ENGINEERING Internal External
20PBCC23 40 60
COURSE OUTCOMES
On completion of the course, the studs will be able to
· CO1: understand the principles and techniques leads to comprehensive analysis and
practices in Molecular Biotechnology. [K2]
CO2: apply the synthetic information from a wide variety of sources to understand the key
principles of molecular biology and Genetic Engineering. [K3]
CO3: identify the various issues both independently and cooperatively for current and future
research problems in molecular and advanced biotechnology. [K3]
CO4: analyze the various applications of rDNA technology in evolving plants for resistance
to pest and disease, tolerance to herbicides and abiotic factors. [K4]
CO5: evaluate the steps of the synthesis of novel bio products,
development of research aptitude and technical skills. [K5]
UNIT I
Structural aspects of DNA Duplex: DNA Replication – Various models. Enzymes involved in
DNA replication, Events in replication fork, Mechanism of Bacterial and Plasmid DNA
replication. Inhibitors of replication. Errors during replication, Mutation & its types.
(15 Hours)

59 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT II
Transcription & Translation: Enzymes involved in transcription – DNA dependent RNA
polymerase – Mechanism involved in transcription. Inhibitors of transcription, Operon model –
lac, Ara& trp. Translation- Enzymes, mechanism of translation. Inhibitors of translation.
Transposons and their types. (15 Hours)
UNIT III
Introduction to restriction modification system: Types, Restriction, Ligation enzymes.
Hemopolymer tailing. Cloning vectors: Gene transfer Vector, expression vectors, plasmid vector-
PBR322, Phage vector – M13 filamentous phage, cosmid, yeast vector – YIP.
Intoduction of rDNA into host cells- E-coli, plant cells and Mammalian Cells. Techniques involved
in transfer of genes. Blue white selection, phenotypic selection, selection based on hybridization
technique, HRT & HART.
(15 Hours)
UNIT IV
Expression of cloned proteins: Maximizing the expression of cloned genes – vectors for
maximizing the product – promoter expression system- T7 expression system and ara expression
system, Constructing the optimal promoter, increasing the plasmid copy number.
Gene manipulation in plants: Gene transfer through Agrobacterium tumefaciens, Protoplast
isolation and its applications, protoplast fusion , Bt Cotton, production of herbicide, virus & pest
resistance plants. Transgenic plants; Experimental procedure for producing transgenic plants.
(15 Hours)
UNIT V
Production of novel proteins: Human insulin, somatostatin, Interferon, Vaccines, Blood proteins,
Lymphokines. Transgenic animals: Methods of production, Expression of foreign DNA in
transgenic mice. Gene therapy: Treating adenosine deaminase deficiency (combined immune
deficiency)

(15 Hours)
TEXT BOOKS
1. R.C.Dubey and D.K.Maheswari (2014), A text book of Biotechnology, 5th edition ,S
Chand Publishing.
2. V.Kumaresan (2012), Biotechnology ,6th edition, Saras Publication.

60 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS

1. David Freifelder(2005), Molecular biology, 2nd Edition, Narosa publishing house,

New Delhi.

2. Watson, J. D., Hopkins, N. H., Roberts, J. W., Steitz, J. A., & Weiner, A. M.( 1992).
Molecular biology of the Gene , 4/e, The Benjamin/Cumming Publishing Company Inc.

3. Adams (1986), Biochemistry of nucleic acids, 10th Edition, Springer.

4. Old and primrose (2004), Principles of Gene manipulation, 6th edition, Blackwell science
Ltd, USA.

5. T.A.Brown (2006), Gene cloning, 5th Edition, Wiley-Blackwell, USA.

6. S.N.Jogdand (2008), Gene Biotechnology, 2nd Edition, Himalaya publication.
7. Sandhya Mitra (1996), Genetic Engineering-Principles and practice, Macmillan India Ltd,
New Delhi.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course
Code
20PBCC23 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 M M M L L L - - - -

CO2
M M L M M M M - - -
CO3 H H H H H H M - - -

CO4 H H H H M M M M M -
CO5
H H M H M H H H M M

Dr.P.Annapoorani
Dr.P.Annapoorani Mrs.M.Rajakumari
Head of the Department Course Designer

61 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)

Semester II Hours/Week: 5

Core Course- 6 Credits: 4

MOLECULAR BIOLOGY AND GENETIC
Course Code ENGINEERING Internal External
20PBCC23N 40 60

COURSE OUTCOMES
On completion of the course, the studs will be able to
· CO1: understand the principles and techniques leads to comprehensive
analysis and practices in Molecular Biotechnology. [K2]
CO2: apply the synthetic information from a wide variety of sources to understand the
key principles of molecular biology and Genetic Engineering. [K3]
CO3: identify the various issues both independently and cooperatively for
current and future research problems in molecular and advanced biotechnology. [K3]
CO4: analyze the various applications of rDNA technology in evolving plants for
resistance to pest and disease, tolerance to herbicides and abiotic factors. [K4]
CO5: create and explain the steps involved in the synthesis of novel bio products,
development of research aptitude and technical skills. [K5]
UNIT I
Structural aspects of DNA Duplex: DNA Replication – Various models. Enzymes involved in
DNA replication, Events in replication fork, Mechanism of Bacterial and Plasmid DNA
replication. Inhibitors of replication. Errors during replication, Mutation & its types. (15 Hours)

UNIT II
Transcription & Translation: Enzymes involved in transcription – DNA dependent RNA
polymerase – Mechanism involved in transcription. Inhibitors of transcription, Fine structure of
gene, Operon models – lac, ara & trp. Translation- Enzymes, mechanism of translation. Inhibitors of
translation. Transposons and its types. (15 Hours)
62 17th Academic Council Meeting 31.01.2023
 Curriculum for M.Sc. BIOCHEMISTRY
UNIT III

Introduction to restriction modification system: Types, Restriction, Ligation enzymes.

Hemopolymer tailing. Cloning vectors: Gene transfer Vector, expression vectors, plasmid vector-
PBR322, PUC18, BAC, Phage vector – M13 filamentous phage, cosmid, yeast vector – YIP, YAC.
Introduction of rDNA into host cells- E-coli, plant cell and Mammalian Cells. Techniques involved
in transfer of genes. Blue white selection, phenotypic selection, selection based on hybridization
technique, HRT & HART. (15 Hours)
UNIT IV
Expression of cloned proteins: Maximizing the expression of cloned genes – vectors for
maximizing the product – promoter expression system- T7 expression system and ara expression
system, Constructing the optimal promoter, increasing the plasmid copy number, Synthetic Biology
(E. coli and Yeast).
Gene manipulation in plants: Gene transfer through Agrobacterium tumefaciens, Protoplast
isolation and its applications, protoplast fusion , Bt Cotton, production of herbicide, virus &
pest resistance plants. Transgenic plants; Experimental procedure for producing transgenic plants.
(15Hours)
UNIT V
Production of novel proteins: Human insulin, somatostatin, Interferon, Vaccines, Blood
proteins, Lymphokines. Transgenic animals: Methods of production, Expression of foreign
DNA in transgenic mice, Gene Knock out and Knock in (Humanized mice Antibody). Gene
therapy: Treating adenosine deaminase deficiency (combined immune deficiency) .
(15 Hours)
TEXT BOOKS
1. R.C.Dubey and D.K.Maheswari (2014), A text book of Biotechnology, 5th edition ,S. Chand
Publishing.
REEFERENCE BOOKS
1. David Freifelder(2005), Molecular biology, 2nd Edition, Narosa publishing house, New
Delhi.
2. Watson, J. D., Hopkins, N. H., Roberts, J. W., Steitz, J. A., & Weiner, A. M.( 1992).
Molecular biology of the Gene , 4/e, The Benjamin/Cumming Publishing Company Inc.
3. Adams (1986), Biochemistry of nucleic acids, 10th Edition, Springer.
4. T.A.Brown (2006), Gene cloning, 5th Edition, Wiley-Blackwell, USA.
5. S.N.Jogdand (2008), Gene Biotechnology, 2nd Edition, Himalaya publication.

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 Curriculum for M.Sc. BIOCHEMISTRY

6. Sandhya Mitra (1996), Genetic Engineering-Principles and practice, Macmillan

India Ltd, New Delhi.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
(20PBCC23N)
1a 1b 2 3a 3b 4 5 6 7 8

CO 1 H H H M M H M M L M

CO 2 H M M H M H M H M M
CO 3 H H H H H H M H H H

CO 4 H H H H M M M H H M

CO 5 H H M H M H H H H M

Dr.P.Annapoorani
Dr.P.Annapoorani Mrs.M.Rajakumari
Head of the Department Course Designer

64 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2020 -21 onwards)
Semester: II Hours/Week: 6

Core Practical-2 MICROBIOLOGY AND MOLECULAR Credits: 3

BIOLOGY TECHNIQUES LAB
Course Code Internal External
20PBCC21P 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: apply the principles and methodologies of microbial cell culture, characterization of
microbes, electrophoresis , DNA isolation from various cells such as bacterial cells
and animal tissues. [K3]
CO2: outline the flow charts for microbes characterization, electrophoresis, DNA isolation
from various cells [K3]
CO3: observe and interpret the results of microbiology and molecular biology experiments
using the standard methods and techniques and complete the record work. [K3]
CO4: Comment on the microbial cell culture, characterization of microbes, electrophoresis
And DNA isolation from various cells. [K4]
CO5: assess the modifications in the use of reagents, in characterizing microbes, in
electrophoresis and DNA isolation. [K5]
Experiments
1. Preparation and use of glassware, sterilization
2. Preparation of simple microbial culture media
3. Maintenance of microbial cultures
4. Metabolite study:
i) Hydrogen sulfide production
ii) Acid production
5. Electrophoresis i) SDS-PAGE
ii) Agarose gel electrophoresis

65 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

6. Isolation of genomic DNA animal tissue/ intact chloroplast

/coconut endosperm i)DNA-Quantification
ii) Hyperchromic effect and Tm

REFERENCE BOOKS
1. Gunasekaran.P (1995) ,Laboratory manual in Microbiology-, New age
International(P) Ltd. Publishers, New Delhi.
2.Aneja, K.R. (1996).Experiments in Microbiology, Plant pathology, Tissue
culture and mushroom Cultivation, I edition, New age International (P) Ltd.
Publishers, New Delhi.
3. PalaniveluP.(2004) Analytical Biochemistry & Separation Techniques
,Palkalai Nagar,
Madurai: 4/e, 21st Century Publication.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCC21P PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 M M M M M M M - M M

CO2 H H M M M M M - M H

CO3 H H H H H H H L M H

CO4 H H H H H H H L M H

CO5 H H H H H H H M M H

Dr.P.Annapoorani Mrs. R.Gloria Jemmi Christobel

Head of the Department Course Designer

66 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2020 - 2021 onwards)
Semester II Hours/Week: 4
Core practical-3 Credits: 2
Course Code BIOINFORMATICS LAB Internal External
20PBCC22P 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: write the protocols for sequence retrieval from different Biological databases. [K3]
CO2: construct the methodologies for accessing proteomic tools and DNA or protein
sequence similarity search using BLAST and visualizing protein structure. [K3]

CO3: interpret the results of retrieved and aligned sequences from different primary
databases, structural databases, multiple sequence alignment, proteomic tools and
complete the record work notebook. [K3]
CO4: assess the basic informatics tools to extract or retrieve information from
Biological databases, molecular visualization tools and its applications. [K4]
CO5: build homology model for unknown protein sequence and predict the structure. [K5]

Experiments

● Access EBI query website for Downloading a file

● Accessing of Genbank website and downloading of files
● Accessing EMBL website and cross link to Genbank
● Accessing DDBJ website for Downloading a file
● Accessing SWISS-Prot database and Downloading a protein sequence
● Accessing PDB website and Downloading a protein structure
● Search NCBI‟s MMDB

67 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
● USE THE Dali structure family Database
● BLAST similarity search for Nucleotide sequences
● BLAST similarity search for protein sequences
● Fasta similarity search for Nucleotide sequences
● Fasta similarity search for Protein sequences
⮚ Find difference between insulin sequence in pig and human Accessing EBI website for
Downloading an Entry of Human Lysosomal alpha Glucosidase Gene
⮚Show that sequence coding for C-peptide is less conserved than sequences coding for the A-
chain and the B-chain
● Searching and retrieving from KEGG
● Working with Ensembl
⮚ Exploring features related to a gene
⮚ Examining the supporting evidence for a gene prediction.
● ENTREZ AND OMIM
● CHEM Sketch: Small molecules build
⮚ Draw the structure of any chemical compound using Chem Sketch
⮚ Find its properties
● Multiple Sequence Alignment – ClustaLw
● Expasy-proteomic tools
● Visualize a protein structure
⮚ Rasmol
⮚ Homology modeling
● NCBI‟s 1000 Genomes Browser-Introduction
⮚ Data viewer: Navigate exons and find CDS
positions
REFERENCES BOOKS

1. Mani.K(2000), Bioinformatics – a practical approach, 3rd edition, published by Saras

Publication, Nagercoil.

2. Sundaralingam.R , Kumaresan.V(2001), Bioinformatics , 3rd edition, published by Saras

Publication, Nagercoil.

68 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCC22P
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H M M M M M M - - L
CO2 H H M M M M M - - L
CO3 H H M H H H H H H H
CO4 H H M H H H H H H H
CO5 H H M H H H H H H H

Dr.P.Annapoorani Mrs. K.Sudha Rameshwari

Head of the Department Course Designer

69 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2020 - 2021 onwards)
Semester II Hours/Week: 5
DSEC-2 Credits: 4
Course Code PLANT BIOCHEMISTRY Internal External
20PBCE21 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: summarize plant tissue culture, organelles of plant cell and also
biochemistry of photosynthetic process and its relation to man and its
environment. [K2]
CO2: interpret the role of nutrients and secondary metabolites in plants. [K3]
CO3: sketch the effect of environmental factors, growth regulators and
pathogens in plant physiology. [K3]
CO4: analyze the biochemical pathways involved in the synthesis, transport,
growth, maturation and disease resistant mechanisms in plants. [K4]
CO5: evaluate the transport mechanism, tissue culture technique and industrial
applications of secondary metabolites in plants. [K5]
UNIT I
Plant Cell & Transport Mechanism: Structure. Composition and functions of plant cell
organelles, including cell wall and cell membranes. Biosynthesis of cell wall. Plant and tissue
culture. Water management, ascent of sap, mechanisms for movement of solutes. Translocation in
xylem and phloem. (15 Hours)

UNIT II

Plant Nutrition: Essential nutrients – inorganic nutrients, their functions, deficient and
toxicity symptoms. Nitrogen fixation Biochemistry of nitrate assimilation sulphur metabolism,
sulphur activation and assimilation.
(15 Hours)
70 17th Academic Council Meeting 31.01.2023
 Curriculum for M.Sc. BIOCHEMISTRY

UNIT III

Photosynthesis: Structure and composition of photosynthetic apparatus light and dark reactions-
Photophosphorylation; Carbondioxide fixation C3, C4 and CAM pathways. Biosynthesis of
sucrose and starch, Factors affecting the rate of photosynthesis. Photorespiration.
(10 Hours)

UNIT IV

Growth Regulators & Phytochemistry: Auxins, Gibberellins, Cytokinins, ABA, Ethylene

Metabolism, functions and mechanism of action. Plant growth inhibitors. Plant chemicals
and their significance storage carbohydrates, proteins and fats. Secondary plant products and
their economic importance- waxes; essential oils, phenolic glycosides, flavones anthocyanins and
alkaloids. (15 Hours)

UNIT V

Biochemistry of plant diseases: Plant pathogenesis initial stages of pathogenesis, mechanisms of

pathogenesis- Mechanism of attack. Responses of plants to pathogens- pathological effects of
respiration, photosynthesis, cell wall enzymes and –water uptake. Disease-resistance mechanism;
phytoalexins. Photomorphogenesis: Photoperiodism – phytochrome, Physiology of flowering,
Physiology and biochemistry of fruit ripening, Physiology and biochemistry of senescence
Biochemistry of seed germination. (20 Hours)
TEXT BOOKS
1. R.K. Sinha. (2012). Modern Plant Physiology, 4th Edition, Alpha Science International Ltd
2. Pandey & Sinha. (2012), Modern Plant Physiology, 4th Edition. Vikas Publication
House Pvt Ltd.
REFERENCE BOOKS
1. Thomas Moore. (2010). Biochemistry and physiology of plant hormones, II
Edition, Springer-Verlag Berlin and Heidelberg GmbH & Co.
2. Devlin. (2009). Plant Biochemistry, Fourth Edition, Vikas Publication House Pvt Ltd.

71 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCE21 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H H M M H M M M H
CO2 H M M M M H M M - -
CO3 H H M M M M M M - L
CO4 H H M H M M M M - -
CO5 H H M H H H H M H M

Dr.R.Renuka
Dr.P.Annapoorani Dr.R.Salini
Head of the Department Course Designer

72 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)
Semester II Hours/Week: 5
DSEC-2 PLANT BIOCHEMISTRY Credits: 4
Course Code Internal External
20PBCE21N 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: summarize plant tissue culture, organelles of plant cell and also
biochemistry of Photosynthetic process and its relation to man and its environment. [K2]

CO2: interpret the role of nutrients and secondary metabolites in plants. [K3]
CO3: sketch the effect of environmental factors, growth regulators and
pathogens in plant physiology. [K3]
CO4: analyze the biochemical pathways involved in the synthesis, transport,
growth, maturation and disease resistant mechanisms in plants. [K4]
CO5: evaluate the transport mechanism, tissue culture technique and industrial
applications of secondary metabolites in plants. [K5]
UNIT I
Plant Cell & Transport Mechanism: Structure. Composition and functions of plant cell
organelles, including cell wall and cell membranes. Biosynthesis of cell wall. Plant tissue culture.
Transport Mechanisms-Water management, ascent of sap, mechanisms for movement of solutes.
Translocation in xylem and phloem. (15 Hours)

UNIT II
Plant Nutrition: Essential nutrients – inorganic nutrients, their functions, deficient and
toxicity symptoms. Nitrogen fixation- Biochemistry of Nitrate assimilation, Sulphur
metabolism, Sulphur Activation and Assimilation. (15 Hours)

73 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT III
Photosynthesis: Structure and composition of photosynthetic apparatus, light and dark
reactions- Photophosphorylation; Carbon-di-oxide fixation C3, C4 and CAM pathways.
Biosynthesis of sucrose and starch, Factors affecting the rate of photosynthesis.
Photorespiration-Photosynthesis and plant productivity.
(10 Hours)
UNIT IV
Growth Regulators & Phytochemistry: Auxins, Gibberellins, Cytokinins, ABA, Ethylene
Metabolism, functions and mechanism of action. Plant growth inhibitors. Plant chemicals and
their significance storage carbohydrates, proteins and fats. Secondary plant products and their
economic importance- waxes; essential oils, phenolic glycosides, flavones, anthocyanins and
alkaloids. Biosynthesis of alkaloids, terpenoids, phenolics and pigments (general treatment
only). Algal secondary metabolites. (15 Hours)

UNIT V
Biochemistry of plant diseases: Plant pathogenesis initial stages of pathogenesis, mechanisms of
pathogenesis- Mechanism of attack. Responses of plants to pathogens- pathological
effects of respiration, photosynthesis, cell wall enzymes and –water uptake. Disease-resistance
mechanism; phytoalexins. Photomorphogenesis: Photoperiodism – phytochrome, Physiology of
flowering, Physiology and biochemistry of fruit ripening, Physiology and biochemistry of
senescence Biochemistry of seed germination. Plant Stress, Plant responses to abiotic and biotic
stress. (20 Hours)
TEXT BOOKS
1. Lincoln Taiz and Eduardo Zeiger, (2002). Plant Physiology, Sinauer Associates; 3rd edition
2. Pandey & Sinha. (2012), Modern Plant Physiology, 4th Edition. Vikas Publication
House Pvt Ltd.
REFERENCE BOOKS
1. Thomas Moore. (2010). Biochemistry and physiology of plant hormones, II
Edition, Springer-Verlag Berlin and Heidelberg GmbH & Co.
2. Devlin. (2009). Plant Biochemistry, Fourth Edition, Vikas Publication House Pvt Ltd.
3. R.K. Sinha. (2012). Modern Plant Physiology, 4th Edition, Alpha Science International
Ltd

74 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
(20PBCE21N) 1a 1b 2 3a 3b 4 5 6 7 8
CO 1 H H M M M H M M M L
CO 2 H M M M M H M M L L
CO 3 H H M M M M M M L L
CO 4 H H M H M M M M M L
CO 5 H H M H H H H M H M

Dr.R.Renuka
Dr.P.Annapoorani Dr.R.Salini
Head of the Department Course Designer

75 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 - 2021 onwards)
Semester II Hours/Week: 5
BIOINFORMATICS AND
DSEC- 2 Credits: 4
Course Code NANOTECHNOLOGY Internal External
20PBCE22 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand the basic bioinformatics techniques and synthesis,
applications of nanomaterials used in biological research. [K2]
CO2: apply sequence alignment methods for sequence similarity search, visualization
tools in biological data and different types of nanomaterials, applications of
Nanotechnology in Biomedical and Pharmaceutical Industries. [K3]
CO3: analyze the different types of nano materials and application of
Nanotechnology in Biomedical and Pharmaceutical Industries and different
databases, tools used in biological analysis. [K3]
CO4: examine the development, ELSI of Genome projects, challenges, scope and
application of bioinformatics, importance of scoring matrix, gap penalty in
sequence alignment, properties of nanomaterials, different types of nanoparticle
synthesis methods and its advantage, disadvantage. [K4]
CO5: evaluate sequence analysis using tools in biological systems, important
contributions in bioinformatics, goals, strategics of human genome project, role
of nanotechnology in biological research and industries. [K5]

UNIT I
History, Scope and Importance: Important contributions - Aims and tasks of Bioinformatics -
applications of Bioinformatics - challenges and opportunities - internet basics – HTML –
introduction to NCBI data model - Various file formats for biological sequences. Databases -
Tools and their uses Primary sequence databases - Composite sequence databases - Secondary

76 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

databases- Nucleic acid sequence databases - Protein sequence data bases – Structural databases-
Protein structure visualization tools (RasMol, Swiss PDB Viewer) (15 Hours)

UNIT II

Sequence Alignment Methods: Sequence analysis of Biological data - Significance of Sequence

alignment – Pairwise sequence alignment methods - Use of Scoring matrices and Gap penalties in
sequence alignments - Multiple sequence alignment methods – Tools and application of
multiple sequence alignment. Definition of genome and genomics. Types of gene - map genetic,
cytogenetic and physical. Molecular markers for mapping - RFLPs, microsatellites and SNPs.
Assembling a physical map of the genome – chromosome walking and jumping. (15 Hours)

UNIT III
Proteomics and Genomics: Genome projects: E.coli, D.melanogaster, A. thaliana and
mouse. The human genome project: goals, mapping strategies, markers, sequencing technologies,
results of final sequence, potential benefits and risks, ethical, legal and social issues (ELSI).
(15 Hours)
UNIT IV
Introduction to Nanotechnology: Introduction, Definition, and Nanoscale, Classification of
Nanomaterials: Quantum Dots, Wells and Wires. Carbon-based Nanomaterials - Nanotubes, Metal
based Nanomaterials (Nanogold, Nanosilver and metal oxides). Properties of nanostructured
materials.
(15 Hours)
UNIT V
Synthesis and applications of Nanomaterials: Top-down (Nanolithography, CVD), Bottom-up
(Sol-gel processing, chemical synthesis). Biological methods of Synthesis: Use of Plant extracts,
bacteria, fungi, yeast and other biological particles. Applications of Nanotechnology in
Biomedical and Pharmaceutical Industries. (15 Hours)

TEXT BOOKS

1. S.C. Rastogi & others (2003), Bioinformatics - Concepts, Skills, and

Applications, CBS Publishing.

2. T K Attwood, D J parry – Smith (2005), “Introduction to Bioinformatics”, Pearson Education,

1st Edition, 11th Reprint.

77 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

3. Pradeep.T (2007). Nano: The Essentials Understanding Nanoscience and Nanotechnology,

1st Edition, Tata McGraw – Hill Publishing Company Limited, New Delhi.

REFERENCES BOOKS

1. Andreas D Baxevanis& B F Francis (2000), "Bioinformatics - A practical guide to analysis of

Genes & Proteins ", John Wiley.
2. LakshmanDesai (2007), Nanotechnology, 1st Edition, Paragon International Publishers.
3. C S V Murthy, (2003,) “Bioinformatics”, Himalaya Publishing House, 1st Edition.
4. S. Ignacimuthu, S.J., (1995). “Basic Bioinformatics”, Narosa Publishing House,
5. C.S. Tsai, (2002), An Introduction to Computational Biochemistry, Wiley Liss, New York.

Course PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCE22
1a 1b 2 3a 3b 4 5 6 7 8

CO1 H H M L L L M M - -

CO2 H M M L L L M M - -

CO3 H H M H M M M M - M

CO4 H H M H M M M M - M

CO5 H H M H M M M M - M

Dr.P.Annapoorani Mrs.K.Sudha Rameshwari

Head of the Department Course Designer

78 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester II Hours/Week: 5
DSEC- 2 MOLECULAR MECHANISM OF Credits: 4
Course Code INFECTIOUS DISEASES Internal External
20PBCE23 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: demonstrate the mechanism of disease cause, transmission, detection,
treatment and prevention. [K2]
CO2: apply the mechanism behind various diseases in diagnosis and research for treatment
Interventions. [K3]
CO3: identify the reason behind the disease cause, transmission and the response towards
Treatment. [K3]
CO4: analyze the existing or emerging infection, drug resistance mechanisms in
order to develop new tools for their management. [K4]
CO5: interpret the research findings pertaining to transmission, detection, treatment and
prevention of diseases. [K5]

UNIT I

Overview of infectious diseases : Infectious agents - Bacteria, Viruses, protozoa and

fungi, pathogenicity and virulence; Facultative / obligate intracellular pathogens.

(15 Hours)

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 Curriculum for M.Sc. BIOCHEMISTRY

UNIT
II
Bacterial disease : Epidemiology, signs and symptoms, causative agent, history, infection and
pathogenicity, Diagnostics, Therapeutics and vaccines. Drug resistance, mechanisms, Multidrug
efflux pumps, extended spectrum β-lactamases and implications on public health. Molecular
mechanisms for Tuberculosis, Typhoid, Cholera. (15 Hours)

UNIT
III
Viral diseases : Epidemiology, signs and symptoms, causative agent, history, infection and
pathogenesis, Detection, Drugs and inhibitors, Vaccines. Molecular mechanisms for
hepatitis, influenza, dengue, polio, herpes.
(15 Hours)

UNIT
IV
Parasitic diseases : Epidemiology, signs and symptoms, causative agents, history, Vectors,
life cycle, Host parasite interactions, Diagnostics, Drugs and Inhibitors, Resistance,
Vaccine development. Molecular mechanisms for Malaria.
(15 Hours)

UNIT
V
Emerging and re-emerging infectious diseases and pathogens: MRSA, SARS virus, Bird
flu, prions, Hemorrhagic Fever and Chlamydiae, opportunistic fungal pathogens. (15
Hours) TEXT BOOKS

1. Principles and practices of Infectious diseases, 7th edition, Mandell, Douglas

and
Bennett. S, Volume, 2. Churchill Livingstone Elsevier. ISBN: 978-0-443-06839-3

REFERENCE
BOOKS
1. Klein's Microbiology (2008) 7th Ed., Prescott, Harley, Willey, J.M.,
Sherwood, L.M., Woolverton, C.J. Mc Graw Hill International Edition (New
York) ISBN:
978-007-126727.

80 17th Academic Council Meeting 31.01.2023

2. Sherris Medical Microbiology: An Introduction to Infectious Curriculum
Diseases. for M.Sc. BIOCHEMISTRY

(2010).
3. Kenneth J.Ryan, C. George Ray, Publisher: McGraw-Hill. ISBN-13:
978-
0071604024 ISBN-10: 0071604022 Medical Microbiology. (2012).
4. Patrick R. Murray, Ken S. Rosenthal, Michael A.Pfaller, Elsevier Health
Sciences.
ISBN: 978-0-323-08692-9.
5. Bacterial Pathogenesis: A molecular approach by Salyers AA and Whitt DD
eds.
American Society for Microbiology Press, Washington, DC USA. 2002

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCE23
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 M M H M M M H - - -

CO2 H H H H H M H - L H

CO3 H H H H H M H M L H

CO4 H H H H H H H M M H

CO5 H H H H H H H M M H

Dr.P.Annapoorani Mrs. R.Gloria Jemmi Christobel

Head of the Department Course Designer

81 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY

(2020 -2021onwards)
Semester III Hours/Week: 6
Core Course-7 Credits: 5
IMMUNO CHEMISTRY
Course Code Internal External
20PBCC31 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to·
CO1: understand the structure and function of the major lymphoid systems,
including the molecular, biochemical and cellular mechanisms for
maintaining homeostasis and basic defense mechanism. [K2]
CO2: apply the immunoglobulin genes of the immune system, antigen antibody
interaction and their applications in various immuno assays. [K3]
CO3: develop the knowledge of each lymphoid system „s contribution to the functioning of
health and diseases. [K3]
CO4: analyze the mechanism of cellular and molecular basis of immune
responsiveness and its therapeutic implications in human system. [K4]
CO5: evaluate the state-of-the-art experimental methods and Technologies in disease
diagnosis and clinical research. [K5]
UNIT I
Principles of immunology: Types of immunity. Antigens – Factors influencing antigenicity.
Specific group of antigens- self antigens, foreign antigens- tumor antigens, viral antigens, bacterial
antigens, haptens. Organs of the immune system. Cells of the immune system, response of B cells
to antigens, response of T cells to antigens, Interaction between T and B cells. Cytokines and
Lymphokines (Briefly). Mononuclear phagocytic system.
(20 Hours)

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 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II

Antibodies and Complement: properties of antibody, structure of IgG, Isotopes, allotypes,

idiotype Igs as antigens. Monoclonal antibodies (hybridoma). Ag-Ab complex- chemical basis of
Ag-Ab binding, affinity, valence, kinetics of Ag-Ab reactions. Theories of antibody formation;
generation of antibody diversity and its genetics. Complement system-complement pathway and
complement fixation. Disorders of immunoglobulin synthesis.
(20 Hours)
UNIT III

Vaccines and Hypersensitivity: Vaccination- Passive and active immunization schedule,

antibacterial, antitoxic and antiviral vaccines - Corona viral Vaccine. Allergy and
hypersensitivity: Type I, II, III, IV hypersensitivity. Autoimmunity: Rheumatoid arthritis,
myasthenia gravis, Systemic lupus erythematosus and Type I Diabetes mellitus.
(15 Hours)
UNIT IV
Transplantation and Tumour immunology: Transplantation- Graft rejection,
transplantation antigens, Graft vs Host disease. prevention of graft rejection, Immuno
suppressive agents, Immune surveillance mechanism. Tumor immunology. Acquired
Immuno Deficiency Syndrome.
(20 Hours)
UNIT V
Immuno histochemistry: Principles, Procedure and Applications-
Precipitation, Agglutination, Immuno-electrophoresis, Fluorescent antibody techniques, RIA ,
ELISA, ELISpot, FACS .
(15 Hours)
TEXT BOOK
1. Janis kuby,(2007). Immunology, New York.: W.H.Freeman and Company, 6th Edition.
REFERENCE BOOKS
1. Tizard (1995). Immunology, USA: Harcourt Brace College Publishers, 4th Edition.
2. Ivan M. Roitt and Peter J. Delves, (2005). Essential Immunology, USA: Blackwell Publication,
10th Edition.
3. James T.Barrett (1988). Text Book of Immunology - An Introduction to
Immunochemistry & Immunobiology, Mosby: 5th Edition.
4. Donald Weir (1997). Immunology, Churchill Livingstone, United Kingdom,8th Edition.H
83 17th Academic Council Meeting 31.01.2023
 Curriculum for M.Sc. BIOCHEMISTRY

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCC31 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 H H H - - M M M M -

CO2 H M H M H M M M - -

CO3 H H M H M M M - - M

CO4 H H H H M M H - M -

CO5 M H H H M H H M - -

Dr.P.Annapoorani Mrs.M.Rajakumari
Head of the Department Course Designer

84 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020-2021 onwards)
Semester III Hours/Week: 6
Core Course-8 Credits: 5
BIOSTATISTICS
Course Code Internal External
20PBCC32 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: demonstrate the necessary skill sets to interpret statistical data. [K2]
CO2: utilize effective statistical methods for data analysis and
interpretation. [K3] CO3: discover the statistical methodology for the
evaluation during health and disease conditions. [K3]
CO4: assess the general theory of data analysis and specific concepts as they
apply to confidence intervals, effect sizes and hypothesis testing. [K4]
CO5: hypothesize study designs for analyzing data based on research problems. [K5]

UNIT I
Sampling and tests of significance: sampling distribution, standard error. Tests of significance for
attributes-test for number of success, test for proportion of success and test for difference between
proportions. Tests of significance of large samples: testing the significance of mean, testing the
difference between means of two samples, testing the difference between two standard
deviations. Students„t‟ distribution: Testing the significance of the mean of a random sample,
testing the difference between means of two samples and testing the significance of
observed correlation coefficient. (Problems Only).Steps in hypothesis testing. (20 Hours)

85 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT II

Theoretical distributions-Binomial distribution –Types-Properties-Binomial distribution-Fitting of

Binomial distribution, Poisson distribution-Fitting of Poisson distribution, Normal distribution
Methods of constructing normal distribution-The Ordinate method, The Area method(Problems Only)
(15 Hours)

UNIT III
Types of Correlation - positive & negative: Simple, partial & negative: Linear and non-linear
correlation (Definition Only).Scatter diagram method, Graphic Method, Karl Pearson‟s
coefficient of correlation, rank correlation coefficient .Regression equation and regression lines
(problems only). Difference between Correlation and Regression. (20 Hours) UNIT IV

Analysis of frequencies and analysis of variance: Chi-square-Test for goodness of fit,

Test

for independence of attributes; yate‟s correction, analysis of variance (ANOVA)- Single factor
ANOVA and Two-factor ANOVA with unequal and equal replication. (Problems Only).

(20 Hours)
UNIT V
Research methodology: Concepts of research, types of study design-case control study, nested
case control study, Familial study. Layout of thesis-review of literature, Referencing-styles of
Reference. Plagiarism (Short note). (15 Hours)
TEXT BOOKS
1. Palanichamy.S and Manoharan.M (2008). Statistical Methods for

Biologists(Biostatistics),Palani: Palani Paramount Publications, 3rd Edition.

2 . Gupta.S.P. (2008). Statistical Methods, New Delhi: Sultan Chand & Sons, 36th

Edition.

REFERENCE BOOK
1.Kothari, R.C. (1993). Research Methodology, New Delhi: Wiley Eastern Limited, 5th
Edition.

86 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCC32
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H M H L H M - - -

CO2 H H H H M H M L - M

CO3 H H H H M H M M M M

CO4 H H H H M H H M M H

CO5 H H H M H H H H M H

Dr.P.Annapoorani Mrs.R.Gloria Jemmi Christobel

Head of the Department Course Designer

87 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2023 -2024 onwards)
Semester III Hours/Week: 6
Core Course-8 Credits: 5
BIOSTATISTICS
Course Code Internal External
20PBCC32N 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1 : demonstrate the necessary skill sets to interpret statistical data. [K2]
CO2: utilize effective statistical methods for data analysis and interpretation. [K3]
CO3: discover the statistical methodology for the evaluation during health and
disease conditions. [K3]
CO4: assess the general theory of data analysis and specific concepts as they apply
to confidence intervals, effect sizes and hypothesis testing. [K4]
CO5:evaluate various statistical methods for data analysis . [K5]
UNIT I
Representation of Data: Diagrammatic- Simple bar diagram, Rectangles, squares, circles or Pie
diagram, Graphic representation-Histogram, Frequency Polygon, Frequency Curve, Cumulative
Frequency Curve or O Give Curve. Measures of central tendency (mean, median, mode) and
dispersion (standard deviation and mean deviation). Sampling: Types of sampling, Advantages
and disadvantages. R-Studio
(15Hours)
UNIT II
Sampling and tests of significance: standard error. Tests of significance for attributes-test for
number of success test for proportion of success and test for difference between proportions. Tests
of significance of large samples: testing the significance of mean, testing the difference between
means of two samples, testing the difference between two standard deviations. Students„t‟
distribution: Testing the significance of the mean of a random sample, testing the difference
between means of two samples and testing the significance of observed correlation coefficient.
(Problems Only). Steps in hypothesis testing. (20 Hours)
88 17th Academic Council Meeting 31.01.2023
 Curriculum for M.Sc. BIOCHEMISTRY
UNIT III
Theoretical distributions-Binomial distribution –Types-Properties-Binomial distribution-Fitting
of Binomial distribution, Poisson distribution-Fitting of Poisson distribution, Normal
distribution Methods of constructing normal distribution-The Ordinate method, The Area method
(Problems Only) (15 Hours)

UNIT IV
Types of Correlation - positive & negative: Simple, partial & negative: Linear and non-linear
correlation (Definition Only).Scatter diagram method, Graphic Method, Karl Pearson‟s
coefficient of correlation, rank correlation coefficient. Regression equation and regression
lines (problems only). Difference between Correlation and Regression. (20 Hours)
UNIT V
Analysis of frequencies and analysis of variance: Chi-square-Test for goodness of fit, Test for
independence of attributes; yate‟s correction, analysis of variance (ANOVA)- Single factor
ANOVA and Two-factor ANOVA with unequal and equal replication. (Problems Only).
(20 Hours)
TEXT BOOKS
1. Palanichamy.S and Manoharan.M (2008). Statistical Methods for Biologists(Biostatistics),
Palani: Palani Paramount Publications, 3rd Edition.
2. Gupta.S.P. (2008). Statistical Methods, New Delhi: Sultan Chand & Sons, 36th Edition.
REFERENCE BOOK
1.Kothari, R.C. (1993). Research Methodology, New Delhi: Wiley Eastern Limited, 5th Edition.
2. Babak Shahbaba. (2012) Biostatistics with R -An Introduction to Statistics Through Biological
Data. ISBN: 978-1-4614-1302-8.

PO1 PO 2 PO3 PO4 PO5 PO 6 PO7 PO8

Course Code
20PBCC32N 1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H H H H M M H M M
CO 2 H H M H H H H H M M
CO 3 M M M M H H H H H H
CO 4 H H M H H H H H M M
CO 5 H H M H H H H H H H

Dr.P.Annapoorani
Dr.R.Salini
Head of the Department Course Desi

89 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester III Hours/Week: 6
EUKARYOTIC GENE
Core Course-9 Credits: 5
EXPRESSION
Course Code Internal External
20PBCC33 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: outline the cell division process, stem cell types, genome organization and molecular
mechanisms in gene expression and regulation in eukaryotes. [K2]
CO2: apply the properties of stem cells, gene function and their modulation in various aspects of
normal and abnormal signaling pathways. [K3]
CO3: make use of the perceived knowledge about gene and protein expression on various signaling
mechanism and its deregulation in the oncogenesis process. [K3]
CO4 : analyze the properties of stem cells , normal and abnormal gene regulation and protein
expression during cell cycle ,cell signaling, and oncogenesis mechanism. [K4]
CO5 : assess the information related to eukaryotic gene expression , stem cells , cell division, cell
signaling and cancer. [K5]
UNIT I
Genome Organization- Eukaryotic genome organization, Repeat sequences- Cot curve analysis,
structural DNA sequences- Complexity, frequency of repetitions. Nucleosomes, chromatin - active
chromatin, inactive chromatin, heterochromatin and euchromatin. (20 Hours)

UNIT II
Replication & Transcription- Eukaryotic gene replication, Transcription of RNAs, mRNA
structure, processing of mRNA- capping, splicing and editing. Promoters, Cis-regulatory
elements, Enhancers, trans – acting proteins. Difference between prokaryotic and eukaryotic
transcription. DNA repair. Difference between prokaryotic and eukaryotic replication.
(20 Hours)

90 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT III
Epigenetics- Chromatin remodeling, Role of histones and its modifications, non-histone proteins.
Introduction to Silencing mechanism- DNA methylation. Role of epigenetic mechanism in
normal development and oncogenesis. Protein and gene microarray technology -Principles and
applications.
(20 Hours)
UNIT IV
Translation-Genetic code, eukaryotic ribosomes, eukaryotic translational process, polysomes,
post translational modification. Secretory proteins-signal hypothesis - Difference between
prokaryotic and eukaryotic translation.
(15 Hours)
UNIT V
Signaling pathways, Cancer and Cell cycle- Signaling Pathways- Stat pathway, MAPK/ERK
Pathway, JNK pathway, Wnt signaling pathway, Notch signaling pathway. Cell cycle,
Oncogenes. Introduction to Stem cells- Properties , Embryonic and adult stem cells.CRISPR-Cas9
technology.
(15 Hours)
TEXT BOOKS
1. David Freifelder (2008). Molecular Biology, New Delhi: Narosa Publications, 2nd Edition.
2. James D.Watson et al., (2004). Molecular Biology of Gene, Pearson Education (Singapore)
Indian Branch, New Delhi. 2nd Edition.
3. Becker, Kleinsmith, Harden (2000). The World of the Cell, United States: Addison Wesley
Longman Inc. 4th Edition.
REFERENCE BOOKS
1. Stephen.L.Wolfe (1993). Molecular and Cellular Biology, USA: Cengage
st
learning Inc. 1 Edition.
2.
Benjamin Lewin (2004). Genes VIII, New Jersey: Pearson Prentice Hall,
8th Edition.

3. Darnell, Lodish, Baltimore (1986). Molecular Cell Biology, United

States: WH Freeman & Company, 1st Edition.
4. Brown. T.A. (2006). Genomes, Garland Science Inc. United States, 3rd Edition.

91 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

5.Lodish et.al.(2004). Molecular Cell Biology, United States: WH Freeman & Company,
5th Edition.
6.De Robertis and De Robertis (2001). Cell and Molecular Biology,Wolters Kluwer India Pvt
Ltd. 8th Edition.
7.Gerald Karp (2004). Cell and Molecular Biology, New York: John Wiley & Sons. 4thedition

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO 8

20PBCC33
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 M H M L L - - - - -
CO2 M H H H H M H - - H
CO3 H H H H H M H - L H
CO4 H H H H H H H - L H
CO5 H H H H H H H H M H

Dr.P.Annapoorani
Dr.P.Annapoorani Mrs.R.Gloria Jemmi Christobel
Head of the Department Course Designers

92 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2023 -2024 onwards)
Semester III Hours/Week: 6
EUKARYOTIC GENE
Core Course-9 Credits: 5
EXPRESSION
Course Code Internal External
20PBCC33N 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to

CO1: demonstrate the cell division process, stem cell types, genome organization and
molecular mechanisms in gene expression and regulation in eukaryotes. [K2]
CO2: apply the properties of stem cells, gene function and their modulation in various
aspects of normal and abnormal signaling pathways. [K3]
CO3: apply the perceived knowledge about gene and protein expression on various
signaling mechanism and its deregulation in oncogenesis process. [K3]
CO4 : analyse and discuss research articles in the area of normal and
abnormal gene regulation and protein expression, stem cells & cell
cycle process. [K4]
CO5 : evaluate the information relating to eukaryotic gene expression, cell division ,
stem cells, cell signaling and cancer. [K5]
UNIT I
Genome Organization- Eukaryotic Genome Organization, Repeat Sequences- Cot
Curve Analysis, C-Value Paradox, Structural DNA Sequences- Complexity, Frequency Of
Repetitions. Nucleosomes, Chromatin - Active Chromatin, Inactive Chromatin, Heterochromatin
and Euchromatin. (20 Hours)

93 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II
Replication & Transcription- Eukaryotic Gene Replication, Transcription of RNAs.
Structure of mRNA & tRNA .RNA Processing-Capping, Polyadenylation, Splicing- Types of
Splicing, Snurps and RNA editing. Promoters, Enhancers, Cis-regulatory elements, Trans - acting
proteins. Difference between Prokaryotic and Eukaryotic Replication and Transcription. DNA
repair. (20 Hours)

UNIT III
Epigenetics- Chromatin remodeling, Role of Histones and its modifications, Non-histone
proteins. Introduction to Silencing mechanism- DNA methylation. Role of Epigenetic
mechanism in normal development and Oncogenesis. Protein and Gene Microarray Technology -
Principles and Applications. (20 Hours)

UNIT IV
Translation-Genetic Code, Eukaryotic Ribosomes, Eukaryotic Translational process,
Polysomes, Post Translational Modification. Secretory Proteins-Signal hypothesis . Difference
between Prokaryotic and Eukaryotic translation. (15 Hours)

UNIT V
Signaling pathways, Cancer and Cell cycle- Signaling Pathways- Wnt signaling pathway,
Notch signaling pathway, ROS pathway, Protein Kinase B, NF-kB and AMP-Activated Protein
Kinase. Cell cycle- Phases and Check points. Oncogenesis Mechanism. Introduction to Stem
cells- Properties, Embryonic and adult stem cells. CRISPR-Cas9 technology.
(15 Hours)

TEXT BOOKS
1. David Freifelder (2008). Molecular Biology, New Delhi: Narosa Publications, 2nd
Edition.
2. James D.Watson et al., (2004). Molecular Biology of Gene, Pearson Education
(Singapore) Indian Branch, New Delhi. 2nd Edition.
3. Becker, Kleinsmith, Harden (2000). The World of the Cell, United States: Addison
Wesley Longman Inc. 4th Edition.

94 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS
1. Stephen.L.Wolfe (1993). Molecular and Cellular Biology, USA: Cengage
st
learning Inc.Edition.
1
2. Benjamin Lewin (2004). Genes VIII, New Jersey: Pearson Prentice Hall, 8th
Edition.
3. Darnell, Lodish, Baltimore (1986). Molecular Cell Biology, United States: WH
Freeman & Company, 1st Edition.
4.Brown. T.A. (2006). Genomes, Garland Science Inc. United States, 3rd
Edition.
5.Lodish et.al.(2004). Molecular Cell Biology, United States: WH Freeman &
Company, 5th Edition.
6.De Robertis and De Robertis (2001). Cell and Molecular Biology,Wolters Kluwer
India Pvt. Ltd. 8th Edition.
7.Gerald Karp (2004). Cell and Molecular Biology, New York: John Wiley & Sons. 4thedition

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO 8

20PBCC33N
1a 1b 2 3a 3b 4 5 6 7 8
CO1 M M H H H H M H M M
CO 2 H H M H H H H H M M
CO 3 H M H M H H H H M M
CO 4 H H M H H H H H M M
CO 5 H H M H H H H H M M

Dr.P.Annapoorani
Dr.P.Annapoorani Dr.R.Gloria Jemmi Christobel
Head of the Department Course Designers

95 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester III Hours/Week: 6
Core Practical-4 IMMUNOLOGY AND Credits: 3
ADVANCED BIOCHEMISTRY
Course Code LAB Internal External
20PBCC31P 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: apply the principles of western blot, immunochemical techniques,
cloning and restriction digestion. [K3]
CO2: sketch the schema chart for the techniques involved in immunology and
advanced biochemistry. [K3]
CO3: observe and interpret the results of molecular biology and immunological
techniques and complete the record work. [K3]
CO4: comment and compare the antigen-antibody interaction methods and gene
cloning technique. [K4]
CO5: Assess gene cloning with and without restriction endonucleases, ligase,
plasmid and judge the antigen-antibody interaction. [K5]
EXPERIMENTS
1. Aggultination and precipitiation
2. Immunoelectrophoresis
3. Immunodiffusion
4. ELISA [demonstration]
5. Western Blot analysis – commercial kit
6. Isolation of Plasmid DNA from E.coli Bacteria.
7. Single Restriction digestion of λ DNA and ligation
8. Double restriction digestion of λ DNA.
9. Cloning of DNA fragment by blue, white selection method
10. Determination of antibody titre

96 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS

1. Adrian.J.Harwood (1996). Basic DNA and RNA Protocols, NewYork, USA: Humana Press,
1st Edition.
2. Stanley R.Maloy (1990). Experimental Techniques in Bacterial Genetics, Boston: Jones and
Bartlett Publisher, 1st Edition.
3. Richard.L.Myers (1994). Immunology: A Laboratory Manual, United States: McGraw-Hill
Science, 2nd Edition.
4. Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J.G.
Seidman, John A. Smith & Kevin Struhl (2003). Current Protocols in Molecular
Biology, United
States: John Wiley & Sons Inc., 1st Edition.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
20PBCC31P
1a 1b 2 3a 3b 4 5 6 7 8
CO1 H H M H H H H M H M
CO2 H H M H H H H M H M
CO3 H H M H H H H M H M
CO4 H H M H H H H M H M
CO5 H H M H H H M M H M

Dr.P.Annapoorani Dr.R.Salini
Mrs.K.Sudha Rameshwari
Head of the Department Course Designer

97 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. Bio Chemistry

(2021 -22 onwards)
Semester III Hours/Week:1
Course Code PRACTICE FOR CSIR NET – Credits: 1
GENERAL PAPER
20PGOL32 Internal External
100 -

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1 : explian various concepts related to numbers, quantitative comparison,
monetary problems and logical reasoning. [K2]
CO2 : apply the analytical skills and logical reasoning in solving problems related to
competitive examinations. [K3]
CO3 : solve typical problems, geometrical type problems, daily life problems in a
effective manner. [K3]
CO4 : analyze the techniques used in solving complicated real life problems. [K4]
CO5 : interpret the data using logical reasoning and observational ability. [K5]

UNIT I
Typical Problems- Series formation
Numerical Ability- Numbers
UNIT II
Geometrical Type Problems
Mensuration and quantitative
comparison
UNIT III
Typical Problems- Moving locomotive problem
Numerical Ability- Distance and Directions

98 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT IV
Daily Life Problems
Finding the X – Average - Monetary problems

UNIT V
Logical Reasoning
Data interpretation – Observational ability – Logical puzzles

BOOKS FOR STUDY:

Christy Varghese (2016)., CSR – NET, General aptitude –A new outlook, Lilly publishing
house, Changanacherry, Kerala

REFERENCE BOOKS
1. Pradip Kumar Ray, General Aptitude Theory ,CSIR NET, Previous question and answer
with explanation and hint to solve, Notion Press, India
2. Ram Mohan Pandey (2021)., CSIR-UGC-NET General Aptitude Theory and
Practice, Pathfinder Publication, a unit of Pathfinder Academy Pvt. Ltd., India.

Unit Chapter Section/Page Number

4 142-162
1
5 163-192
2 12 272-294
3 132-141
3
7 206-220
8 221-230
4 9 231-239
10 240-249
13 295-309
14 310-323
5
15 324-332

99 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

Course code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PGOL32
CO1 H H M M - M - -
CO2 H H H H - M - -
CO3 H H H H - H - -
CO4 H M H H - H - -
CO5 H M H H - H - -

Dr.A.Uma Devi
Dr.A.Uma Devi
Tmty.T.Anitha
Head of the Department Course Designer

100 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020-2021onwards)
Semester IV Hours/Week: 6
Core Course-10 ENVIRONMENTAL Credits: 5
Course Code BIOCHEMISTRY Internal External
20PBCC41 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand the key concepts and complex relationship between biotic and
abiotic components of ecosystem. [K2]
CO2: apply the ecological knowledge and its consequences in basis of regional and
global environmental issues.[K3]
CO3: identify the current environmental problems (Air, water and soil pollution),
chemical pollutants and its impacts on Biosphere. [K3]
CO4: analyze the strategies, technologies and methods for assessment of
environmental system and discuss some mitigation strategies like
Biodegradation and Bioremediation. [K4]
CO5: evaluate basic chemical concepts to analyze the Bio chemical processes involved in
different environmental problems. [K5]
UNIT I
Introduction to Ecosystem-Fundamentals of Ecology, Ecosystems: concept of ecosystems,
energy flow in ecosystems, Food-chains. Interactions between environment and biota - concept of
habitat and ecological niches, Organisms and stress factors - temperature: cold exposure,
acclimatization, heat exposure and adaptation to heat. (15 Hours)

101 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT II

Air, Land and Noise pollution- Types of air pollutants, Sources, effects and control.
Land pollutants & their biochemical effects. Solid Waste- Characteristics of municipal Waste,
disposal of hazardous waste. Noise pollution and their biological effects. Fireworks and match
industry: Pollutants and its abatement. (20 Hours)

UNIT III

Water Pollution & Industrial pollution: Water pollution - Sources, effect and control.
Marine pollution – pollutants, sources, effects, oil pollution -control. Thermal pollution sources,
effects & prevention. Removal of waste water from sugar industry, paper industry, pesticides &
tannery industry. Bioplastics. Industrial & Laboratory hazards of plastics – biodegradation.
Bioremediation and Bioleaching. (20 Hours)

UNIT IV
Pesticides and Herbicides: Systemic & Non systemic pesticides, structure, mode of
action and applications (2, 4 D, DDT and Malathion only). Behaviour in soil, problems of
pollution by pesticides. Environmental risks of direct & Indirect food additives, food colors &
other contaminants. Occurrence of pesticides in foods .Removal of waste water from
pesticide industry. Biodegradation of pesticides. (20 Hours)

UNIT V
Environmental Toxicology: Environmental carcinogens – chemical carcinogens, classification and
mode of action (Azo dye-Tartrazine, Nitrosamine), Environmental teratogens, teratogenic
effects, mechanism of action of teratogens. Environmental mutagens and their effects. Effects
of radiation – sources of radiation, radioactive waste & management. (15 Hours)

TEXT BOOK
1.Sharma, P.D. (1994). Environmental Biology, Meerut, India: Rastogi & Company, 1st
Edition.

102 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
REFERENCE BOOKS
1. Sharma, B.K. (1994). Environmental Chemistry, Meerut, India: Goel Publishing House,
1stEdition.
2. Jogdand, S.N. (2008). Environmental Biotechnology, Bombay: Himalaya Publishing House,
4th Edition.
3. Sharma, B.K. (2001). Water Pollution, New Delhi: Goel Publishing, 3rd Edition.
4. Paliwal, K.V. (1994). Pesticidal Pollution of Environment and Control, Delhi, India:M.D. \
st
Publications Pvt. Ltd., 1 Edition.
5. Khopkar, S.M. (1994). Environmental Pollution Analysis, London: Heyden & Son Ltd, 1st
Edition.
6. Trivedi, R.N. (1997). A Text Book of Environmental Sciences, New Delhi: Anmol
st
7. Publications, 1 Edition.
8. Michael L. Cain, William D. Bowman, & Sally D. Hacker, (2014). Ecology, New Delhi: Sinauer
Associates Inc, 3rd Edition.

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCC41 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1
M H M M - M M - - M

CO2 H H M H H M M - M -

CO3 H H H H M H M - M M

CO4 H H H H H M H - - -

CO5 H H M H M H H - M M

Dr.P.Annapoorani Mrs.M. Rajakumari

Head of the Department Course Designer

103 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022-2023onwards)

Semester IV Hours/Week: 6
ENVIRONMENTAL
Core Course-10 BIOCHEMISTRY Credits: 5

Course Code Internal External

20PBCC41N 40 60
COURSE OUTCOMES

On completion of the course, the students will be able to

CO1: understand the key concepts and complex relationship between biotic and
abiotic components of ecosystem [K2]
CO2: apply the ecological knowledge and its consequences in basis of
regional and global environmental issues. [K3]
CO3: analyze the current environmental problems (Air, water and soil
pollution), chemical pollutants and its impacts on Biosphere.[K3]
CO4: evaluate the strategies, technologies and methods for assessment of
environmental system and discuss some mitigation strategies like
Biodegradation and Bioremediation.[K4]
CO5: create basic chemical concepts to analyze the Bio chemical processes involved in
different environmental problems.[K5]
UNIT I
Introduction to Ecosystem-Fundamentals of Ecology, Ecosystems: concept of ecosystems,
energy flow in ecosystems, Food-chains. Interactions between environment and biota - concept
of habitat and ecological niches, Organisms and stress factors - temperature: cold exposure,
acclimatization, heat exposure and adaptation to heat. (15 Hours)

104 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II
Air, Land and Noise pollution- Types of air pollutants, Sources, effects and control. Land
pollutants &their biochemical effects. Solid Waste- Characteristics of municipal Waste,
Solid waste management, disposal of hazardous waste. Noise pollution and their biological
effects. Fireworks and match industry: Pollutants and its abatement. Carbon sequestration.
(20 Hours)
UNIT III
Water Pollution & Industrial pollution: Water pollution - Sources, effect and control.
Marine pollution – pollutants, sources, effects, oil pollution -control. Thermal pollution
sources, effects & prevention. Treatment of waste water from sugar industry, paper industry,
pesticides & tannery industry (Physical, Chemical and Biological methods). Effects of radiation
– sources of radiation, radioactive waste & management. Industrial & Laboratory hazards of
plastics, Biodegradation, Bioremediation and Bioleaching. Bioplastics.
(20 Hours)
UNIT IV
Pesticides and Herbicides: Systemic & Non systemic pesticides, structure, mode of action
and applications (2, 4 D, DDT and Malathion only). Behaviour in soil, problems of pollution by
pesticides. Environmental risks of direct & Indirect food additives, food colors & other
contaminants. Occurrence of pesticides in foods. Treatment of waste water from pesticide
industry. Biodegradation of pesticides.
(20 Hours)

Environmental Toxicology and convention : Environmental carcinogens – chemical

carcinogens, classification and mode of action (Azo dye-Tartrazine, Nitrosamine),
Environmental teratogens, teratogenic effects, mechanism of action of teratogens.
Environmental mutagens and their effects. Chipko movement, Montreal Protocol, Kyoto
Protocol, Ramsar Convention, Stockholm Convention, Rio summit.
(15 Hours)

TEXT BOOK
1.Sharma, P.D. (1994). Environmental Biology, Meerut, India: Rastogi & Company, 1st Edition

105 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS
1. Sharma, B.K. (1994). Environmental Chemistry, Meerut, India: Goel Publishing House,
1stEdition.
2. Jogdand, S.N. (2008). Environmental Biotechnology, Bombay: Himalaya Publishing House,
th
4 Edition.
3. Sharma, B.K. (2001). Water Pollution, New Delhi: Goel Publishing, 3rd Edition.
4. Paliwal, K.V. (1994). Pesticidal Pollution of Environment and Control, Delhi, India:M.D.
Publications Pvt. Ltd., 1st Edition.
5. Khopkar, S.M. (1994). Environmental Pollution Analysis, London: Heyden & Son Ltd,
1st Edition. Trivedi, R.N. (1997). A Text Book of Environmental Sciences, New Delhi: Anmol
Publications, 1st Edition.
6. Michael L. Cain, William D. Bowman, & Sally D. Hacker, (2014). Ecology,
New Delhi: Sinauer Associates Inc, 3rd Edition.

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
(20PBCC41N)
1a 1b 2 3a 3b 4 5 6 7 8

CO 1 M H M H H H M L L M

CO 2 H M M H H M H M M H

CO 3 H M H H M H M M M M

CO 4 H M H H M M M H H H
CO 5 M M M M M M M M M M

Dr.P.Annapoorani Mrs.M. Rajakumari

Head of the Department Course Designer

106 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester IV Hours/Week: 6
Core Course-11 CLINICAL BIOCHEMISTRY Credits: 5
Course Code Internal External
20PBCC42 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: discuss the diagnostic importance of gastric, renal function, immunological,
prenatal test and metabolic functions. [K2]
CO2: perform clinical investigation on blood, urine and other body fluids for diagnostic purpose
[K3]
CO3. determine the abnormalities of digestive, reproductive, kidney system and inborn
errors of metabolism. [K3]
CO4: interpret the laboratory results with respect to the biological reference ranges and
infer the results critically in light of the clinical picture. [K4]
CO5: assess the role of clinical biochemistry monitoring the biochemical basis of diseases and
novel strategies to prevent the diseases [K5]

UNIT I
Disorders of carbohydrate and lipid metabolism: Diabetes mellitus, Hypoglycemia, Hyper
lipoproteinemia and hypolipoproteinemia.

Disorders of inborn error of metabolism: Metabolic disorders of Carbhohydrate-galctosemia, glucogen

storage disease (GSD). Metabolic disorders of aminoacids- Phenylketonuria, Alkaptonuria, Maple syrup
urine disease. Metabolic disorders of lipids: Tay-sach‟s disease, Fabry‟s disease, Niemann-Pick disease,
Gaucher‟s disease, Refsum‟s disease, Krabbe‟s disease. (20 Hours)

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 Curriculum for M.Sc. BIOCHEMISTRY
UNIT II

Disorders of digestive system: Typhoid, Cholera, Crohn disease, Appendicitis, Hernia –causes,
symptoms, diagnosis, treatment and prevention.

Gastric function tests: collection of gastric contents, examination of gastric residium, Fractional Test
Meal (FTM), stimulation tests, tubeless gastric analysis. Peptic Ulcer -definition, types, causes, diagnosis,
prevention, treatment. Difference between stomach cancer and stomach ulcer. (20 Hours)

UNIT III

Kidney and urine- Collection of urine, Routine qualitative analysis of normal and abnormal constituents
of urine and its diagnostic importance – reducing sugar, blood, bilirubin, ketone bodies, bile salts,
porphyrin, uric acid and protein. Acute and chronic glomerulonephritis, acute and chronic renal failure.
Renal function test, renal calculi. (20 Hours)

UNIT IV

Hematology – E.S.R, Screening test for sickle cell anemia, prothrombin time, Bleeding time.
Immunological test:C- reactive protein test, rheumatoid arthritis test, immunologic test for pregnancy.
Body fluid: Cerebrospinal fluid – site of withdrawal, blood brain barrier, collection of sample, function of
CSF, composition of normal CSF and clinical investigation of CSF in various CNS diseases.

(15 Hours)
UNIT V
Disorders of reproductive system: Amnorrhea, cervical cancer, Poly cystic ovarian disease (PCOD),
Endometriosis, Menopause, Breast cancer, Ectopic pregnancy- causes, symptoms, diagnosis, treatment
and prevention.
Prenatal diagnosis of diseases- Amniotic fluid and fetal blood examination. Acetylcholinesterase and
other tests on amniotic fluid. Chromosomal abnormalities by cytogenetics. Newborn screening: β-
Thalassemia, PKU, cystic fibrosis and sweat tests. (20 Hours)
TEXT BOOKS
1. Chatterjea, M. N. & Rana Shinde (2011). Text book of Medical Biochemistry, New Delhi:
Jaypee Brothers Medical Publishers (P) Ltd, 8th Edition.
2. Deb, A.C. (2001). Fundamentals of Biochemistry, Kolkata: New Central Book Agency,
7th Edition.
3. Kanai L Mukherjee &Swarajit Ghosh (2010). Medical Laboratory Technology,New Delhi:
Vol I, Tata Mcgrawhill, 2nd Edition.

108 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
REFERENCE BOOKS
1. Harrison (1994). Principles of Internal Medicine,McGraw-Hill Companies, United States:
13th Edition.
2. Sonntag & Oswald ( 2002). Tietz Fundamentals of Clinical Chemistry, WB Saunders
Philadelphia. 5th Edition.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCC42 PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 H H H M M M M M M -
CO2 H H M M M M M M M -
CO3 H H H M H M - M M M
CO4 H H M M H M M M M H
CO5 H H H M H M H M M H

Dr.R.Mallika
Dr.P.Annapoorani Mrs.K. Sudha Rameshwari
Head of the Department Course Designers

109 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022-2023onwards)

Semester IV Hours/Week: 6
CLINICAL BIOCHEMISTRY
Core Course-11 Credits: 5

Course Code Internal External

20PBCC42N 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to

CO1: discuss the diagnostic importance of gastric, renal function, immunological,

prenatal test and metabolic functions. [K2]
CO2: perform clinical investigation on blood, urine and other body fluids for diagnostic purpose
[K3]
CO3. determine the abnormalities of digestive, reproductive, kidney system and inborn
errors of metabolism. [K3]
CO4: interpret the laboratory results with respect to the biological reference ranges and
infer the results critically in light of the clinical picture. [K4]
CO5: assess the role of clinical biochemistry monitoring the biochemical basis of diseases and
novel strategies to prevent the diseases [K5]

UNIT I
Disorders of carbohydrate and lipid metabolism: Diabetes mellitus, Hypoglycemia, Hyper
lipoproteinemia and hypolipoproteinemia .
Disorders of inborn error of metabolism: Metabolic disorders of Carbhohydrate - galctosemia,
glucogen storage disease (GSD). Metabolic disorders of aminoacids- Phenylketonuria, Alkaptonuria,
Maple syrup urine disease. Metabolic disorders of lipids: Tay-sach‟s disease, Fabry‟s disease, Niemann-
Pick disease, Gaucher‟s disease, Refsum‟s disease, Krabbe‟s disease. (20 Hours)

110 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II
Disorders of digestive system: Typhoid, Cholera, Crohn disease, Appendicitis, Hernia –causes,
symptoms, diagnosis, treatment and prevention. Gastric function tests: collection of gastric contents,
examination of gastric residium, Fractional Test Meal (FTM), stimulation tests, tubeless gastric analysis.
Peptic Ulcer-definition, types, causes, diagnosis, prevention, treatment. Difference between stomach
cancer and stomach ulcer. (20 Hours)

UNIT III
Kidney and urine- Collection of urine, Routine qualitative analysis of normal and abnormal constituents
of urine and its diagnostic importance – reducing sugar, blood, bilirubin, ketone bodies, bile salts,
porphyrin, uric acid and protein. Acute and chronic glomerulonephritis, acute and chronic renal failure.
Renal function test, renal calculi. (15 Hours)
UNIT IV
Hematology – E.S.R, Screening test for sickle cell anemia, prothrombin time, Bleeding time.
Immunological test: C- reactive protein test, rheumatoid arthritis test, immunologic test for pregnancy.
Body fluid: pericardial fluid, synovial fluid, pleural fluid, Cerebrospinal fluid –Collection, composition,
clinical significances. (15 Hours)
UNIT V
Disorders of reproductive system: Amnorrhea, cervical cancer, Poly cystic ovarian disease (PCOD),
Endometriosis, Menopause, Breast cancer, Ectopic pregnancy- causes, symptoms, diagnosis, treatment
and prevention.
Prenatal diagnosis of diseases- Amniotic fluid and fetal blood examination. Acetylcholinesterase and
other tests on amniotic fluid. Chromosomal abnormalities by cytogenetics. Newborn screening: β-
Thalassemia, PKU, cystic fibrosis and sweat tests. (20 Hours)
TEXT BOOKS
1. Chatterjea, M. N. & Rana Shinde (2011). Text book of Medical Biochemistry, New Delhi:
Jaypee Brothers Medical Publishers (P) Ltd, 8th Edition.
2. Deb, A.C. (2001). Fundamentals of Biochemistry, Kolkata: New Central Book Agency,
7th Edition.
3. Kanai L Mukherjee &Swarajit Ghosh (2010). Medical Laboratory Technology,New Delhi:
Vol I, Tata Mcgrawhill, 2nd Edition.

111 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

REFERENCE BOOKS
1. Harrison (1994). Principles of Internal Medicine,McGraw-Hill Companies, United States:
13th Edition.
2. Sonntag & Oswald ( 2002). Tietz Fundamentals of Clinical Chemistry, WB Saunders
Philadelphia. 5th Edition.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course Code
20PBCC42N PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 H H H M M M M M M -
CO2 H H M M M M M M M -
CO3 H H H M H M - M M M
CO4 H H M M H M M M M H
CO5 H H H M H M H M M H

Dr.R.Mallika
Dr.P.Annapoorani Mrs.K. Sudha Rameshwari
Head of the Department Course Designer

112 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.SC. BIOCHEMISTRY
(2020 -2021 onwards)
Semester IV Hours/Week: 6

Core Course-12 DEVELOPMENTAL BIOLOGY Credits: 5

Course Code AND GENETICS Internal External

20PBCC43 40 60
COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: summarize the principles and experimental approaches of developmental biology and
genetics. [K2]
CO2: compute the principles of genetics , molecular events of embryogenesis in understanding
human embryo development and associated defects. [K3]
CO3: make use of molecular laboratory techniques used routinely in embryo development,
forensic analysis including sex typing, DNA profiling, Single Nucleotide Polymorphism
detection , DNA sequencing and genetic disorders. [K3]
CO4: analyse the findings of embryo development process, human genome project, genetic
counseling and mendelian principles.[K4]
CO5: evaluate the early development process, principles of genetics to produce a family
pedigree from a family history, and to distinguish patterns of inheritance for genetic
disorders linked to autosomes or sex chromosomes. [K5]
UNIT I
Basic concepts of Developmental Biology: Theories of developmental biology. General concepts of
organism development: Potency, commitment, specification, induction, competence, determination &
differentiation; morphogenetic gradients; cell fate & cell lineages; genomic equivalence and cytoplasmic
determinants. (15 Hours)

113 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

UNIT II
Gametogenesis, Fertilization, Cleavage & Gastrulation: Spermatogenesis and oogenesis
process; types of sperms and eggs. Fertilization- approach of sperm to the egg- Activation of egg –
Cortical reaction. Cleavage pattern in mammals. Blastulation and Gastrulation in mammals. Neural
tube formation and cell migration in mammals.
(20 Hours)
UNIT III

Mendelian Genetics: Mendel‟s study of heredity, Phenotype, Genotype, Dominant and

Recessive alleles, Principle of dominance, Principle of segregation, Principle of independent
assortment. Monohybrid crosses, Dihybrid crosses, Trihybrid crosses, Test Cross, Back cross.
Alleles, Co-dominant alleles, Multiple alleles . Linkage and crossing over in Drosophila.
(20 Hours)

UNIT IV

Human Genetics: Pedigree analysis, Linkage analysis, Chromosome mapping. Human disorders
following Mendelian patterns of inheritance. Genome imprinting. Gene amplification. STRs and
VNTRs, Paternity test. (20 Hours)

UNIT V

Inherited disorders: Polyploidy, aneuploidy , Allosomal (Kline felter‟s syndrome and Turner
syndrome) Autosomal (Down syndrome). Genetic counseling. Human genome project.

(15 Hours)

TEXT BOOKS
1. Arumugam.N. (2003). A Text Book of Embryology, Nagercoil, TamilNadu: Saras
Publication, 1st Edition.
2. Varma P.S. &Agarwal.V.K (2001). Genetics, New Delhi: S.chand Publications,5th Edition.
3. Subramanian.M.A. (2012). Developmental Biology, Chennai: MJP Publishers, Ist Edition.

114 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
REFERENCE BOOKS
1. Balinsky (1981). An Introduction to Embryology, Philadelphia: W.B. Saunders Company,
5th Edition.
2. Berill, N.J.(1971). Developmental Biology, London: MC Graw Hill, 1st Edition.
3. Saunder.J.W. (1982). Developmental Biology – Pattern and Principles,
Newyork: Macmillan.
4.James D.Watson Et al., (2004). Molecular Biology of Gene, New Delhi: Pearson Education
(Singapore) Indian Branch, 2nd Edition.
5.Eldon John Gardner (1991). Principles of Genetics, USA: John Wiley & Sons, 8th Edition.
6. Bhatnagar, S.M. (1995). Essentials of Human Genetics, Hyderabad: Sangam Books Pvt. Ltd.

1st Edition.

7. Robert H.Tamarin (2001). Principles of Genetics, Newyork: MCGraw Hill Companies,

7th Edition.

Course Code PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
20PBCC43
PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8
CO1 M H M L L - - - - -

CO2 H H H H H M L - - H

CO3 H H H H H H H H - H

CO4 H H H H H H H H L H

CO5 H H H H H H H H L H

Dr.P.Annapoorani Mrs.R.Gloria Jemmi Christobel

Head of the Department Course Designer

115 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)
Semester IV Hours/Week: 6
DEVELOPMENTAL BIOLOGY
Core Course-12 AND GENETICS Credits: 5

Course Code Internal External

20PBCC43N 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to

CO1: summarize the principles and experimental approaches of developmental

biology and genetics. [K2]
CO2: apply the genetics principle, molecular events of embryogenesis in understanding
human development and associated defects. [K3]
CO3 : apply molecular laboratory techniques used routinely in embryo development,
forensic analysis including sex typing, DNA profiling, Single Nucleotide
Polymorphism detection and in DNA sequencing. [K3]
CO4: examine the early development process, principles of genetics to produce a
family pedigree from a family history, and to distinguish patterns of
inheritance for genetic disorders linked to autosomes or sex chromosomes. [K4]
CO5 : evaluate the concept of genetics, human genome project, genetic counselling,
fertilization process and embryo development, genetic markers . [K5]
UNIT I
Basic concepts of Developmental Biology: Theories of developmental biology. General
concepts of organism development: Potency, commitment, specification, induction,
competence, determination & differentiation; morphogenetic gradients; cell fate & cell lineages;
genomic equivalence and cytoplasmic determinants. Transgenics in analysis of development,
Programmed Cell Death (15 Hours)

116 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
UNIT II
Gametogenesis, Fertilization, Cleavage & Gastrulation: Spermatogenesis and oogenesis
process; types of sperms and eggs. Fertilization- approach of sperm to the egg- Activation of
egg – Cortical reaction. Cleavage pattern in mammals. Blastulation and Gastrulation in mammals.
Neural tube formation and Cell migration in mammals. (20 Hours)
UNIT III
Mendelian Genetics: Mendel‟s study of heredity-Mendelian Laws. Pedigree analysis- Sex
Linked Pedigree, Autosomal Pedigree, Importance of Pedigree Analysis. Linkage analysis,
Chromosome mapping. Linkage and crossing over in Drosophila.
(20 Hours)
UNIT IV
Human Genetics: Human disorders following Mendelian patterns of inheritance. Gene Markers-
STRs and VNTRs. Karyotype, Chromosome Banding. Genome Imprinting. Gene amplification.
Paternity test. Next Generation Sequencing. (20 Hours)
UNIT V
Inherited disorders: Polyploidy, aneuploidy , Allosomal -Klinefelters‟ syndrome and Turners‟
syndrome. Autosomal -Down syndrome. Genetic counseling. Human genome project. Online
Mendelian Inheritance in Man. (15 Hours)
TEXT BOOKS
1 . Arumugam.N. (2003). A Text Book of Embryology, Nagercoil, TamilNadu: Saras Publication,
1st Edition.
2. Varma P.S. &Agarwal.V.K (2001). Genetics, New Delhi: S.chand Publications,5th Edition.
3. Subramanian.M.A. (2012). Developmental Biology, Chennai: MJP Publishers, Ist Edition.
REFERENCE BOOKS
1. Balinsky (1981). An Introduction to Embryology, Philadelphia: W.B. Saunders Company,
5th Edition.
2. Berill, N.J.(1971). Developmental Biology, London: MC Graw Hill, 1st Edition.
3 Saunder.J.W. (1982). Developmental Biology – Pattern and Principles,
Newyork: Macmillan.
4.James D.Watson Et al., (2004). Molecular Biology of Gene, New Delhi: Pearson Education
(Singapore) Indian Branch, 2nd Edition.
5.Eldon John Gardner (1991). Principles of Genetics, USA: John Wiley & Sons, 8th Edition.

117 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

6. Bhatnagar, S.M. (1995). Essentials of Human Genetics, Hyderabad: Sangam Books Pvt. Ltd.
1st Edition.
7. Robert H.Tamarin (2001). Principles of Genetics, Newyork: MC Graw Hill Companies,
7th Edition.

Course Code PO1 PO2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8

20PBCC43N 1a 1b 2 3a 3b 4 5 6 7 8

CO1 M M H H H H M H M M
CO 2 H H M H H H H H M M
CO 3 M M M M H H H H M H
CO 4 H H H H H H H H H H
CO 5 H H M H H H H H H H

Dr.P.Annapoorani Dr.R.Gloria Jemmi Christobel

Head of the Department Course Designer

118 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2020-2021 onwards)

Semester IV Hours/Week: 12
Project Credits: 8
PROJECT VIVA-VOCE
Course Code Internal External
20PBCC41PR 40 60

COURSE OUTCOMES
On completion of the course, the students will be able to

CO1 : Make use of literature review through existing digital platform to formulate the
project work. [K3]
CO2 : Discover the inter disciplinary knowledge to carry out project the
work for the welfare of the society. [K3]
CO3 : Analyze the results of the project work that is being executed and to correlate
them for improving the society. [K4]
CO4 : Develop an insight into the experiments carried out during the project work and
conclude the findings with the existing results. [K5]
CO5 : assess the original findings and interpret the data.[K5]

● Project will be done by the final year students in the fourth semester under the guidance of
respective guides.
● For projects internal marks will be awarded by the respective guide and external marks will be
awarded in the external examinations held at the end of the semester.
● Only individual projects should be allotted.
● The report of the project must be in the prescribed form. It should be typed neatly in MS word
(12 pt, Times New Roman, 1.5 spacing)

● The format of the project report should have the following components.

119 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

❖ First page should contain:

Title of the project report
Name of the candidate
Register number
Name of the supervisor
Address of the institution
Month & year of submission
❖ Contents
❖ Certificate by supervisor
❖ Declaration by candidate
❖ Acknowledgement
❖ Chapters
❖ References
● The project report should be written in 30 - 40 pages.
● Four copies of the project report with binding should be submitted.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8

Course code
20PBCC41PR PSO PSO PSO PSO PSO PSO PSO PSO PSO PSO
1a 1b 2 3a 3b 4 5 6 7 8

CO1 H H H H H H H H M H

CO2 H H H H H H H M M H

CO3 H H H H H H H M M H

CO4 H H H H H H H H M H

CO5 H H H H H H H H M H

Dr.P.Annapoorani Dr.R.Mallika
Head of the Department Course Designer

120 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY

with ‘A’ Grade (3rd Cycle) by NAAC

VIRUDHUNAGAR - 626 001

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)
Semester IV Hours/Week: 12

Core Course-12 Project - Research Methodology & Credits: 8

Ethics
Course Code Internal External
22PBCC41PR 60 40

COURSE OUTCOMES
On completion of the course, the students will be able to

CO1 : apply the knowledge of research problems and hypothesis to carry out the experiments for
Project with Research Ethics. [K3]
CO2 : relate different research papers with their own research problem and design protocols for
their works. [K3]
CO3 : analyze the results of the project work that is being executed and to correlate them for
improving the society. [K4]
CO4 : Develop an insight into the experiments carried out during the project work and conclude
the findings with the existing results. [K5]
CO5 : Summarise the original findings of the project work for paper publication. [K5]

Unit-I: Art of Research planning

Objectives of research – Understanding research and its goals. Critical thinking. Research topic
selection and justification. Techniques involved in designing a questionnaire – Methods of
scientific enquiry – formulation of hypotheses and testing of the same – Development of a
research proposal – Theoretical and Experimental Processes.
(6 hours)

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 Curriculum for M.Sc. BIOCHEMISTRY

Unit-II: Literature survey, Research sources

Sources of information- Literature search: Computers in literature search using Internet websites,
Online data bases – search tools. Literature review – Case studies, review articles and Meta
analysis – record of research review. Ethical and Moral Issues in Research, Plagiarism, tools to
avoid plagiarism – Thesis writing, and Research report writing and preparation of dissertation.
Publication in Journals - ACS-pubs, Royal Society, Springer link, science direct, Wiley –
Interscience ,Pubmed, Elsevier. Submission of research articles for Publication to Reputed journals-
h-index, i-index, ISSN, ISBN –Science Citation Index - Chemistry journal index. Intellectual
Property Rights – Copy right laws – Patent rights. (6 hours)
● Project will be done by the final year students in the fourth semester under the
guidance of respective guides.
● For projects internal marks will be awarded by the respective guide and external
marks will be awarded in the external examinations held at the end of the semester.
● Only individual projects should be allotted.
● The report of the project must be in the prescribed form. It should be typed neatly
in MS word (12 pt, Times New Roman, 1.5 spacing)
● The format of the project report should have the
following components.

First page should contain:

 Title of the project report
 Name of the candidate
 Register number
 Name of the supervisor
 Address of the institution
 Month & year of submission
❖ Contents

❖ Certificate by supervisor

❖ Declaration by candidate

❖ Acknowledgement

❖ Chapters

122 17th Academic Council Meeting 31.01.2023

 Curriculum for M.Sc. BIOCHEMISTRY
❖ References
● The project report should be written in 30 - 40 pages.
● Four copies of the project report with binding should be submitted.
References
1. Ganesan R, Research Methodology for Engineers , MJP Publishers, Chennai. 2011
2. Walpole R.A., Myers R.H., Myers S.L. and Ye, King: Probability &amp; Statistics for
Engineers and Scientists, Pearson Prentice Hall, Pearson Education, Inc. 2007.
3. Anderson B.H., Dursaton, and Poole M.: Thesis and assignment writing, Wiley Eastern
1997.
4. Bijorn Gustavii: How to write and illustrate scientific papers? Cambridge University
Press.
5. Bordens K.S. and Abbott, B.b.: Research Design and Methods, Mc Graw Hill, 2008.
6. Graves N, Varma V: Working for a doctorate Toutledge 1997.
7. Graziano, A., M., and Raulin, M.,L.: Research Methods – A Process of Inquiry, Sixth
Edition, Pearson, 2007.
8. Leedy., P., D.: Practical Research – Planning and Design, Eighth Edition, Pearson., 2005.
9. Kothari C.K., Research Methodology‐ Methods and Techniques ( New Age International,

New Delhi), 2004.

Course PO2 PO 3 PO 4 PO 5 PO PO PO
Code PO1 6 7 8
22PBCC41PR 1a 1b 2 3a 3b 4 5 6 7 8

CO1 H H H H H H H H M H
CO 2 H H H H H H H M M H
CO 3 H H H H H H H M M H
CO 4 H H H H H H H H M H
CO 5 H H H H H H H H M H

Evaluation Pattern (100 marks)

Internal Assessment (60marks) External Assessment (40 marks)
One Periodic Test Project Pre-Submission One Open online Project Viva Voce
(20) Report Presentation Course related to Presentation (10)
(20) (10) the Project (10) (30)

Dr.P.Annapoorani Dr.P.Annapoorani
Head of the Department Course Desiger
123 17th Academic Council Meeting 31.01.2023
 Curriculum for M.Sc. BIOCHEMISTRY

M.Sc. BIOCHEMISTRY
(2022 -2023 onwards)
EXTRA CREDIT COURSE

Semester II Credits: 2

EXTRA CREDIT
BIOSAFETY, LABORATORY
COURSE-1
SAFETY AND IPR
Course Code Internal
22PBCO21 100

COURSE OUTCOMES
On completion of the course, the students will be able to
CO1: understand the biosafety, bioethics and Intellectual Property Rights concepts
CO2: Know-how of issues and bioethics related to molecular technologies and GMOs
CO3: Apply the concept of patenting and process of filing for a patent
CO4: Analyze the use of genetically modified organisms and its effect on human health
CO5: Evaluate the importance of biosafety practices and guidelines in research
Unit-I
Biosafety: Historical background; introduction to biological safety cabinets; primary containment
for biohazards; biosafety levels; recommended biosafety levels for infectious agents and infected
animals; biosafety guidelines - government of India, roles of IBSC, RCGM, GEAC etc. for GMO
applications in food and agriculture; environmental release of GMOs; risk assessment;
risk management and communication; national regulations and international agreements.
Unit-II
Bioethics: Introduction to bioethics, human genome project and its ethical issues, genetic
manipulations and their ethical issues, ethical issues in GMOs, foods and crops in developed and
developing countries, environmental release of GMOs, ethical issues involved in stem cell research
and use, use of animals in research experiments, animal cloning, human cloning and their ethical
aspects, testing of drugs on human volunteers.
124 17th Academic Council Meeting 31.01.2023
 Curriculum for M.Sc. BIOCHEMISTRY
Unit-III
Intellectual Property Rights (IPR): Introduction to patents, types of patents, process involved in
patenting in India, trademarks, copyright, industrial design, trade secrets, traditional knowledge,
geographical indications, history of national and international treaties and conventions on patents,
WTO, GATT, WIPO, Budapest Treaty, Patent Cooperation Treaty (PCT) and TRIPS.
Unit-IV
Patent filing and infringement: Patent application- forms and guidelines, fee structure, time
frames; types of patent applications: provisional and complete specifications; PCT and convention
patent applications, International patenting-requirement, financial assistance for patenting-
introduction to existing schemes; Publication of patents-gazette of India, status in Europe and US.
Unit-V
Patent databases: Searching international databases; analysis and report formation. Indian
Patent Act 1970; recent amendments; filing of a patent application; precautions before patenting
disclosure/non- disclosure; procedure for filing a PCT application.
Research Patenting: Patenting by researchers and scientists-University/organizational rules in
India and abroad. Detailed information on patenting biological products, Case studies on patents
(basmati rice, turmeric, neem etc.), and patent infringement.
Text books
1. Sateesh, M.K., Bioethics and Biosafety, IK International Publishers (2008)
2. Singh I. and Kaur, B., Patent law and Entrepreneurship, Kalyani Publishers (2006).
3. Srinivasan, K. and Awasthi, H.K., Law of Patents, Jain Book Agency (1997)
Reference Books
1. Narayan, P., Patent Law, Eastern Law House (1975).
2. Jonathan, Y.R., Anthology of Biosafety (Vols. 1-4), American Biological Safety

Association (2005).
3. Encyclopedia of Ethical, Legal and Policy issues in Biotechnology, John Wiley & Sons
Inc. (2005)

125 17th Academic Council Meeting 31.01.2023