Sop of gel card method cross
match:-
Gel made up of dextran
acrylamide gel .
LIS (LOW IONIC STRENGTH we use
to reduce the incubation time and
reduce the zeta potential
between the cell.
1. Mentioned patient MR number and bag number on the
gel card
2. Take 2 tube one for the save sample and other for the
suspense of Crossmatch
3. Make suspension from the segment of donor bag .For
segment take 10microlitter blood from the segment
and 50microlitter of LIS solution into tube.
4. Add 50microlitter suspension into marked gel card and
25 microliter serum .
5. Incubate gel card for 15 minutes at 37’C for AHG
6. Run gel card into analyzer for 10 mints at 3500 rpm.
7. Read the result.
Gradating of Crossmatch
4+means one solid button positive reaction
3+means one small button positive reaction
2+ means many Chung of button positive reaction
1+means several Chung’s of button positive reaction.
0 means negative reaction.
SOP FOR A1,B AND O CELL
PREPARATION:-
3 tube with a1cell, b cell and o
cell.
Take 4to5 segments of a1cell,bcell
and o cell.
Wash 3 time all the tubes discard
the supernatant with help of
dropper.
Calculate the amount of alsever
and cell with the help of C1v1
formula .
Take 500ul cell and 9500 ul
alsever With the help of adjuster
Mark the Vail with initials and
DOP.
Cells can use UpTo 7 days .
Sop of weak D testing:-
1. Take 2 tube and mark with patient
MR number and DU ,DUC
2. Make 5 %suspension from the
sample .
3. Add 2to 3 drop of suspension in to
both tube and add anti D into both
tube
4. Spin at IS phase
5. Record the result .
6. Wash the both tube 3 times and at
the end make dry cell button.
7. Add AHG in to the DU and 6
%albumin into DUC
8. Incubate for 15 mints At 37’C
9. Spin at IS Phase.
10. Record the result and add check
cell if the AHG is negative.
SOP OF MANUAL CROSSMATCH :-
1. Mark 5 tubes 2 for XM and remaing 3
for screening cell 1,2 and 3
2. Mark 2 tubes with XM one for saved sample
and one for Cross match
3. NoMention patientMR number and donor bag
number
4. Male 3 to 5% suspension from the donor
bag .
5. Take one drop of suspension of donor and
one drop of patients serum.
6. Run the XM tube at IS phase .
7. In screening cells at screening cell 1 ,2 and 3
and add patient serum into corresponding
tube
8. Add 22% albumin and incubate for 15mints
at 37.c for 15 mints in XM and screen cell
tube and run at 22% albumin phase record
the result
9. Run XM and screening cell tube at AHG
PHASE if AHG PHASE is negative a the check
cell .run at IS PHASE
SOP FOR DAT TESTING:-
1. Mark 2 tubes of DAT and NCDAT with
MR number.
2. Make 3 to5% suspension from purple
top.
3. Take 1 to 2 drop of suspension into
both tube and wash 3 time at the
end make dry red cell button.
4. Add 1 drop of AHG into DAT and 2
drop of 6%albumin into NCDAT .
5. Spin at AHG PHASE
6. Record the result if AHG PHASE is
negative add check cell.
7. Check cell should be MF .
Sop for quality control of blood
product:-
product quality control:-
1. Take near expiry red blood cell bag and strip it 20
times properly
2. Take blood from the segment into tube.
3. The HCT Count should be greater than 55%.
4. Now note the each and very thing on the Quality
control sheet
Formula for conversation of gram into
ml
Weight ÷specific gravity.
Specific gravity of whole blood is
1.06g/ml
Specific gravity of red blood cells is
1.090g/ml
Specific gravity of platelets is 1.04.
Specific gravity for ffp 1.03
Formula for patelets yields:- patelets
count×volume,×10000
For hemolysis take 1 segment blood
and centrifuge for one mint is normal
saline became reddish REDCELL are
hemolysis.
Ideal volume of platelets after
preparation is 15-20ml.
