14/04/25 (Batch B), 16/04/25 (Batch C), 17/04/25 (Batch A)
A 75-year-old man is admitted to the ICU with acute myocardial infarction. He is
 unconscious and put on Foley’s catheter and central venous line. Three days later he
 develops fever and hypotension.
Blood
Nutrient agar
 Shape: round
 Size: 2-4 mm
 Colour: whitish
 Surface: smooth
 Elevation: convex
 Edges: entire
 Opacity: opaque
 Emulsifiability: easily emulsifiable
 Observation: Violet coloured spherical shaped
              bacteria in clusters
 Inference: Gram positive cocci seen
Catalase: Positive
Slide coagulase: Negative
Tube coagulase: Negative
Coagulase negative Staphylococcus spp.
Mannitol fermentation: Not Fermented
Beta haemolysis on blood agar: Negative
Heat-stable nuclease: Negative
CRBSI
04.12.24     A 49-year-old man with no          Initial laboratory study          Clinical features, history of
             medical history presented to       results were notable for a        water logging and laboratory
             hospital for a history of fever,   creatinine of 2.3 mg/dL,          results are suggestive of
             myalgias, and severe bilateral     platelets of 58,000 cells/mm3,    fulminant leptospirosis also
             calf pain that began six days      hemoglobin of 12.8 G/dL,          known as Weil’s disease.
             prior. He denied recent travel and white blood cell count of 8.9 ×
             worked as a construction worker. 103 cells/mm3 with
             He complains of requent            lymphopenia of 2.4%, total
             episodes of water logging at the bilirubin of 4.3 mg/dL, direct
             construction site. Vital signs in bilirubin of 2.6 mg/dL, alkaline
             the emergency department were phosphatase (ALP) of 143 U/L,
             notable for a temperature of       aspartate aminotransferase
             101°F, pulse of 120 beats/min      (AST) of 201 U/L, alanine
             and blood pressure of 156/63 mm aminotransferase (ALT) of 246
             Hg. The patient was alert and      U/L, and creatine kinase of
             oriented. The ocular examination 1219 U/L. The urine analysis
             was notable for scleral icterus. showed moderate hematuria
             The skin appeared jaundiced, the but no proteinuria. Serological
             lungs were clear to auscultation, studies show reactive for
             the abdomen was soft, bilateral Leptospira IgM antibody
             lower extremity tenderness was
             noted and dorsal pedal pulses
             were present bilaterally.
18/03/2025   A seriously ill 60-year-old        Both the blood culture    Clinical presentation is suggestive
             elderly patient was admitted to bottles flagged positive and of catheter related bloodstream
             the ICU of our health setup 5      identification was done   infection (CRBSI)
             days ago. A non-tunneled CVC using Vitek-2 identification
             was in place in the femoral vessel system and identified as
             4 days back. The patient is        Pseudomonas aeruginosa
             having severe fever and chills     on both the specimen.
             since yesterday. His CBC is
             raised and he is having high CRP
             value. The catheter was removed
             and a section of catheter tip was
             sent to the microbiology lab
             along with a peripheral blood
             culture in Bact-Alert automated
             Blood culture bottle.growth of
             catheter tip flashed positive 2.5
             hours prior to the peripheral
             blood culture bottle.
18/03/2025   Mrs. Akshita Mehta, 65-year-old    2 sets of blood culture were    Clinical presentation is suggestive
             female, was admitted CCU after     drawn from central line and    of central line -associated
             having a heart-attack on           peripheral line (mid cubital   bloodstream infection (CLABSI)
             03.08.2022. The next day, a        vein) respectively. Both of    caused by E. coli
             central line was inserted in the   them flagged positive and
             cath-lab. On day 4 of her          were identified as E. coli.
             admission, she developed a fever
             (102°F).