Here are 30 multiple choice questions (MCQs) based on the contents of
your uploaded PowerPoint file titled "9 Lecture Staining". Each question
includes 4 options with the correct answer marked.
1. Who is considered the founder of microscopical staining?
A) Robert Hooke
B) Antonie van Leeuwenhoek
✅ C) Joseph Von Gerlach
D) Rudolf Virchow
2. What did Joseph Von Gerlach introduce as a stain in 1858?
A) Haematoxylin
✅ B) Carmine mixed with gelatin
C) Eosin
D) Orcein
3. Why is staining necessary in histology?
A) To destroy unwanted tissue
B) To magnify tissues
C) To make tissues transparent
✅ D) To produce contrast for interpretation
4. Which cellular component has a high affinity for basic dyes?
✅ A) Nucleus
B) Mitochondria
C) Cytoplasm
D) Cell membrane
5. What dye is commonly used for staining the cytoplasm?
A) Haematoxylin
✅ B) Eosin
C) Saffron
D) Litmus
6. Acidic tissue components stain with:
✅ A) Basic dyes
B) Neutral dyes
C) Acidic dyes
D) Alcohol
7. The physical attachment of dye molecules to tissue components
is called:
A) Solubility
B) Ion exchange
✅ C) Adsorption
D) Extraction
8. Which staining mechanism relies on the solubility of the dye in
different solvents?
A) Direct staining
B) Ion binding
✅ C) Differential solubility
D) Electrostatic staining
9. An example of a dye used in lipid staining is:
✅ A) Sudan Black B
B) Haematoxylin
C) Carmine
D) Litmus
10. Stains derived from natural sources include all EXCEPT:
A) Orcein
B) Litmus
✅ C) Eosin
D) Carmine
11. Which dye is synthetic?
A) Haematoxylin
✅ B) Basic fuchsin
C) Saffron
D) Carmine
12. Synthetic dyes are also referred to as:
A) Metallic dyes
✅ B) Coal tar dyes
C) Alcoholic dyes
D) Natural dyes
13. In acidic stains, the colouring ability lies in the:
✅ A) Acidic component
B) Basic component
C) Alcoholic part
D) Mordant
14. Which stain is used to colour acidic tissue components?
✅ A) Basic stain
B) Acidic stain
C) Neutral stain
D) Alcoholic stain
15. Haematoxylin is an example of a:
A) Natural dye
✅ B) Basic dye
C) Acidic dye
D) Neutral dye
16. Neutral stains are formed by:
✅ A) Mixing acidic and basic dyes
B) Combining two acidic dyes
C) Mixing dyes with alcohol
D) Using only synthetic dyes
17. An example of a neutral stain is:
A) Eosin
B) Haematoxylin
✅ C) Giemsa
D) Saffron
18. What component gives the dye its colour-retaining ability?
✅ A) Auxochrome
B) Mordant
C) Alcohol
D) Chromogen
19. A chromogen differs from a dye because:
✅ A) It does not retain colour in tissues
B) It is colourless
C) It is synthetic
D) It binds covalently
20. What is added to convert chromogen into a dye?
A) Mordant
✅ B) Auxochrome
C) Lipid
D) Cytoplasm
21. The part of the dye that gives colour is the:
A) Mordant
✅ B) Chromophore
C) Auxochrome
D) Fixative
22. Which property is NOT associated with dyes?
A) Salt-forming ability
✅ B) Carbohydrate metabolism
C) Colouring
D) Retention
23. Direct staining is performed:
✅ A) Without a mordant
B) Using only water
C) With a mordant
D) In lipid solvents
24. The role of a mordant is to:
✅ A) Link stain to tissue
B) Intensify colour only
C) Stain the lipid
D) Bleach the stain
25. Substances that increase staining intensity without acting as
mordants are:
✅ A) Accentuators
B) Fixatives
C) Chromogens
D) Mordants
26. Vital staining is performed on:
A) Fixed tissues
✅ B) Living cells
C) Dead cells
D) Alcohol-treated tissues
27. Which staining is performed on living cells still in the organism?
✅ A) Intravital staining
B) Supravital staining
C) Indirect staining
D) Natural staining
28. What type of staining uses a dye and a mordant?
A) Direct
✅ B) Indirect
C) Vital
D) Neutral
29. Which of the following is not a type of classification for stains?
A) Natural vs synthetic
B) Acidic vs basic vs neutral
✅ C) Metallic vs non-metallic
D) None of the above
30. Which dye would be best for staining basic tissue components
like the cytoplasm?
✅ A) Acidic dye
B) Basic dye
C) Neutral dye
D) Mordant-based dye
Would you like a downloadable Word or PDF version of these MCQs, or
maybe an answer key in a table format?
You said:
Myelin Sheeth Staining.pptx
Presentation
ChatGPT said:
Here are 30 multiple choice questions (MCQs) based on the content of
your presentation "Myelin Sheath Staining" by Dr. Muhammad Ilyas. Each
question includes 4 options, with the correct answer marked.
1. Who discovered the myelin sheath?
A) Joseph Von Gerlach
B) Santiago Ramón y Cajal
✅ C) Rudolf Virchow
D) Camillo Golgi
2. What is the main composition of the myelin sheath?
A) 60% lipid, 40% protein
✅ B) 80% lipid, 20% protein
C) 50% lipid, 50% protein
D) 20% lipid, 80% protein
3. The myelin sheath appears white on gross examination because:
A) It is pigmented
✅ B) It is highly refractile
C) It contains melanin
D) It is vascular
4. Which cells produce the myelin sheath in the CNS?
A) Schwann cells
✅ B) Oligodendrocytes
C) Astrocytes
D) Microglia
5. Schwann cells are responsible for myelin formation in the:
A) CNS only
✅ B) PNS
C) Both CNS and PNS
D) Spinal cord only
6. Myelin sheath develops from:
A) Neurons
✅ B) Supporting cells
C) Blood vessels
D) Neuroglial junctions
7. Which of the following stains nerve fibers, leaving the myelin
sheath invisible?
A) Haematoxylin
✅ B) Silver impregnation
C) Osmium tetraoxide
D) Iron alum
8. Which stain leaves nerve fibers invisible but stains the myelin
sheath?
A) Methylene blue
✅ B) Osmium tetraoxide
C) Silver nitrate
D) Sudan black
9. Which vital dye is used to stain nerve fibers?
✅ A) New methylene blue
B) Eosin
C) Giemsa
D) Haematoxylin
10. What is used as a mordant in Haematoxylin staining for myelin?
A) Osmium
✅ B) Iron alum
C) Potassium iodide
D) Ethanol
11. What method uses both mordant and stain in one solution?
A) Nissl method
✅ B) Weil’s method
C) Cajal stain
D) H&E stain
12. Weil’s method stains:
A) Only nerve fibers
✅ B) Myelin sheath
C) Glial cells
D) Cell bodies
13. The recommended fixative for myelin sheath staining is:
A) Formalin
B) Acetone
✅ C) Formol calcium
D) Bouin’s solution
14. Why are thicker sections recommended for nervous tissue
biopsies?
✅ A) To prevent crumbling
B) For easier staining
C) To reduce cost
D) For quicker dehydration
15. What embedding material is suggested for myelin sheath
sections?
A) Paraffin
✅ B) Celloidin
C) Gelatin
D) Resin
16. Myelin sheath staining requires section thickness of:
A) 2-4 µm
✅ B) 10-15 µm
C) 20-25 µm
D) 5-8 µm
17. Which of the following is NOT used in the preparation of
Weigert’s differentiator?
A) Sodium borate
B) Potassium ferricyanide
✅ C) Iron alum
D) Distilled water
18. Which differentiator is a mordant type?
✅ A) Iron alum
B) Weigert’s borax ferricyanide
C) Osmium
D) Silver nitrate
19. Weigert’s differentiator works by:
A) Binding dye to proteins
✅ B) Converting dye to a leucoform
C) Reacting with lipids
D) Melting wax
20. What is the first step in the staining procedure?
✅ A) Dewax and hydrate
B) Add differentiator
C) Mount the section
D) Dry the slide
21. Myelin sheath staining is done at what temperature range?
A) Room temperature
✅ B) 50–60°C
C) 70–80°C
D) 35–40°C
22. How long is the section stained with iron haematoxylin solution?
A) 1–5 min
✅ B) 10–45 min
C) 60 min
D) 5–10 min
23. After staining, what follows next in the procedure?
✅ A) Differentiation in iron alum
B) Clearing in xylene
C) Dehydration
D) Coverslipping
24. Weigert’s differentiator is used:
A) Before staining
B) In place of mordant
✅ C) After iron alum differentiation
D) As a fixative
25. What term is used to describe dye converted into a colourless
form?
A) Auxochrome
✅ B) Leucoform
C) Chromogen
D) Soluble dye
26. The differentiation process in Weil’s method is:
✅ A) Regressive
B) Progressive
C) Accumulative
D) Random
27. What is a key characteristic of iron haematoxylin staining in
Weil's method?
✅ A) Uses iron alum as mordant
B) Direct staining
C) Only one differentiation step
D) Uses eosin
28. The leucoform state refers to:
A) Stained lipid
✅ B) Decolourized dye
C) Mordant-dye complex
D) Denatured protein
29. In which system are Schwann cells NOT involved in myelination?
✅ A) Central Nervous System (CNS)
B) Peripheral Nervous System (PNS)
C) Somatic nerves
D) Autonomic nerves
30. What is the function of celloidin in the staining process?
✅ A) Embedding medium to avoid crumbling
B) Mordant substitute
C) Lipid fixative
D) Differentiator
Let me know if you’d like a combined question paper and answer key, or
formatted document (e.g., PDF, Word).
You said:
Nissl Substance Staining.pptx
Presentation
ChatGPT said:
Here are 30 multiple choice questions (MCQs) based on the presentation
"Nissl Substance Staining" by Dr. Muhammad Ilyas. Each MCQ includes 4
options, with the correct answer marked.
1. Who developed the Nissl staining technique?
A) Rudolf Virchow
✅ B) Franz Nissl
C) Joseph Gerlach
D) Camillo Golgi
2. What is the Nissl substance composed of?
✅ A) Ribonucleoproteins
B) Lipoproteins
C) DNA only
D) Myelin
3. Where is the Nissl substance found in a neuron?
A) Axon only
✅ B) Soma and dendrites
C) Only nucleus
D) Axon hillock
4. Where is Nissl substance absent in neurons?
A) Soma
B) Dendrites
✅ C) Axon and axon hillock
D) Cytoplasm
5. What is the function of the Nissl substance?
A) Lipid synthesis
✅ B) Protein synthesis
C) DNA replication
D) Signal transmission
6. Nissl substance is equivalent to which organelle?
A) Mitochondria
✅ B) Granular endoplasmic reticulum
C) Smooth ER
D) Golgi apparatus
7. Nissl staining is useful for identifying:
A) Myelin sheaths
B) Cell membranes
✅ C) Basophilic substances in neurons
D) Axon terminals
8. Which type of dyes are used to stain Nissl substance?
A) Acidic dyes
✅ B) Basic cationic dyes
C) Neutral dyes
D) Alcoholic dyes
9. Which group do the suitable dyes for Nissl substance belong to?
A) Carmine
✅ B) Aniline group
C) Saffron
D) Hematoxylin group
10. The best dye for staining Nissl substance is:
A) Toluidine blue
✅ B) Cresyl fast violet
C) Eosin
D) Methylene blue
11. Cresyl fast violet stains:
A) Lipids
B) Carbohydrates
✅ C) Nissl substance and nuclei
D) Cell membranes
12. The principle of Nissl staining is based on:
A) Solubility
✅ B) Acid-base reaction
C) Osmotic pressure
D) Precipitation
13. The Nissl material is:
A) Basic
✅ B) Acidic
C) Neutral
D) Non-polar
14. Cresyl fast violet solution is acidified using:
A) Sulfuric acid
✅ B) Acetic acid
C) Hydrochloric acid
D) Nitric acid
15. What is the ideal pH range for Cresyl fast violet solution?
A) 7.0–7.4
✅ B) 3.5–3.8
C) 4.5–5.0
D) 2.0–2.5
16. How much acetic acid is added to 100 ml of cresyl fast violet
solution?
A) 1.0 ml
B) 0.5 ml
✅ C) 0.7 ml
D) 1.5 ml
17. What is the concentration of cresyl fast violet solution used?
A) 1%
✅ B) 0.1%
C) 0.01%
D) 10%
18. The first step in the Nissl staining procedure is:
✅ A) Dewax and hydrate the section
B) Add counterstain
C) Rinse in tap water
D) Observe under microscope
19. What temperature is used during the staining step?
A) 50°C
✅ B) Room temperature
C) 37°C
D) 60°C
20. For how long are sections stained with Cresyl fast violet?
A) 10 min
✅ B) 30 min
C) 5 min
D) 45 min
21. What is used for differentiation after staining?
A) 70% ethanol
✅ B) 95% ethanol
C) Acetic acid
D) Water
22. What follows differentiation in the staining process?
A) Restaining
✅ B) Clearing and mounting
C) Decolorizing
D) Dehydration
23. Nissl bodies are absent in:
A) Soma
B) Dendrites
✅ C) Axon
D) Nucleus
24. Nissl substance appears basophilic because it:
✅ A) Binds to basic dyes
B) Contains lipids
C) Is neutral
D) Is protein-free
25. Which neurotransmitter synthesis is linked to Nissl substance?
A) Dopamine
✅ B) Acetylcholine
C) Serotonin
D) GABA
26. What is a common result of using Cresyl fast violet on neurons?
A) Cytoplasm turns red
✅ B) Granular purple-blue staining
C) Lipid droplets visible
D) Nucleus becomes invisible
27. What property of RNA allows it to bind cresyl fast violet?
✅ A) Its acidic nature
B) Its lipid content
C) Its hydrophobicity
D) Its neutral charge
28. Nissl staining primarily helps in studying:
A) Nerve conduction
✅ B) Neuronal structure
C) Synapse formation
D) DNA repair
29. Cresyl fast violet is best classified as:
A) Acidic dye
✅ B) Basic dye
C) Neutral dye
D) Lipid-soluble dye
30. The invention of Nissl staining was primarily to study:
A) Astrocytes
B) Myelin sheaths
✅ C) Neuronal cytoplasm
D) Capillaries
Would you like all three sets of MCQs (Staining, Myelin Sheath, and Nissl
Substance) compiled into a printable document or formatted as a
quiz/test?
You said:
Metachromatic Staining.pptx
Presentation
ChatGPT said:
Here are 30 multiple choice questions (MCQs) based on your uploaded
presentation "Metachromatic Staining" by Dr. Muhammad Ilyas. Each
question has 4 options, with the correct answer marked.
1. What is metachromasia?
A) The ability of tissue to resist staining
B) Staining tissue with multiple dyes
✅ C) Staining tissue a different colour than the dye's original colour
D) Loss of stain after dehydration
2. What are "metachromatic stains"?
A) Dyes that fade quickly
✅ B) Dyes that stain tissues in a different colour from their original
C) Only acidic dyes
D) Fluorescent dyes
3. Metachromatic staining depends on which two factors?
✅ A) Nature of dye and nature of tissue component
B) Temperature and pH
C) Fixative and embedding medium
D) Thickness of the tissue
4. What is the chemical nature of all metachromatic stains?
A) Acidic
✅ B) Basic
C) Neutral
D) Amphoteric
5. What group do metachromatic stains mainly belong to?
A) Carboxylic group
B) Sulphur group
✅ C) Aniline group of dyes
D) Aldehyde group
6. What is the key property of metachromatic dyes that enables
colour change?
✅ A) Polymerization
B) Solubility
C) Adsorption
D) Diffusion
7. In solution, toluidine blue exists as:
✅ A) Monomers
B) Dimers
C) Polymers
D) None of the above
8. When toluidine blue polymerizes on tissue, the final colour is:
A) Blue
B) Violet
✅ C) Red
D) Green
9. Tissue components showing metachromasia are rich in:
A) Neutral lipids
✅ B) Anionic radicals like phosphates, sulphates
C) Amino acids
D) Fatty acids
10. Glycosaminoglycans are also known as:
A) Neutral mucins
✅ B) Acid polysaccharides
C) Alkaline proteins
D) Basic granules
11. Which of the following is a glycosaminoglycan?
A) Histamine
✅ B) Heparan sulphate
C) Keratin
D) Melanin
12. Which cell has granules that show metachromasia?
✅ A) Mast cell
B) Lymphocyte
C) Neutrophil
D) Eosinophil
13. The main chemical in mast cell granules responsible for
metachromasia is:
✅ A) Heparin
B) Histamine
C) Serotonin
D) Acetylcholine
14. The role of cationic radicals in metachromatic dyes is to:
✅ A) Bind with anionic radicals in tissues
B) Create a negative charge
C) Break down DNA
D) Inhibit protein synthesis
15. What is the approximate distance between anionic radicals that
facilitates metachromasia?
A) 1.0 nm
✅ B) 0.5 nm
C) 0.1 nm
D) 2.0 nm
16. What is the nature of the dual bonding in metachromasia?
✅ A) Acid-base bonding and polymerization
B) Hydrogen and covalent bonds
C) Disulfide bonds and ionic bonds
D) Electrostatic and covalent
17. What happens in the primary reaction of metachromasia?
✅ A) Basic dye binds with acidic tissue
B) Polymerization of tissue
C) Acid dye binds with lipids
D) Protein denaturation
18. What happens in the secondary reaction of metachromasia?
A) Acid binding
✅ B) Polymerization of dye molecules
C) Fixation of proteins
D) Methylation
19. What chemical is stained using toluidine blue in mast cells?
A) Collagen
✅ B) Heparin
C) DNA
D) Myelin
20. What is the function of histamine in mast cells?
A) Clotting agent
✅ B) Vasodilator
C) Metachromatic dye
D) Acid-base buffer
21. Toluidine blue solution is prepared in:
A) Water
✅ B) 50% isopropanol
C) 95% ethanol
D) Acetone
22. What is the concentration of toluidine blue used?
✅ A) 1%
B) 0.5%
C) 2%
D) 0.1%
23. At what temperature should the staining be performed?
A) Room temperature
✅ B) 37°C
C) 60°C
D) 45°C
24. How long should the tissue be stained with toluidine blue?
A) 5–10 minutes
✅ B) 15–20 minutes
C) 30–40 minutes
D) 60 minutes
25. What is used to differentiate after staining with toluidine blue?
A) Water
B) 95% ethanol
✅ C) Absolute isopropanol
D) Acetic acid
26. What is the purpose of differentiation in isopropanol?
✅ A) Clear background by removing excess stain
B) Fix the dye
C) Remove water
D) Bleach the tissue
27. What is used to mount the sections after staining?
A) Glycerin
✅ B) DPX or Canada balsam
C) Wax
D) Alcohol
28. What are proteoglycans made of?
✅ A) Glycosaminoglycans + proteins
B) Collagen + elastin
C) Lipids + proteins
D) DNA + RNA
29. The cytoplasm of mast cells is described as:
A) Homogenous
✅ B) Full of coarse granules
C) Empty
D) Vacuolated
30. Metachromatic red colour indicates what level of
polymerization?
A) Monomer
B) Dimer
C) Trimer
✅ D) Complete polymerization
Would you like me to compile all four MCQ sets into a single document
(PDF or Word) for download or print?
You said:
10 HE stain.pptx
Presentation
ChatGPT said:
Here are 30 multiple choice questions (MCQs) based on the presentation
"Hematoxylin and Eosin (H&E) Staining" by Dr. Muhammad Ilyas. Each
MCQ includes 4 options, with the correct answer marked.
1. H&E stain is primarily used for:
✅ A) Routine histological diagnosis
B) Immunohistochemistry
C) DNA sequencing
D) Microbiology cultures
2. Hematoxylin is derived from which plant?
✅ A) Haematoxylon campechianum
B) Eucalyptus
C) Acacia
D) Rhubarb
3. Who first used hematoxylin in staining?
A) Rudolf Virchow
✅ B) Wilhelm von Waldeyer-Hartz
C) Camillo Golgi
D) Franz Nissl
4. What is the chemical formula of hematoxylin?
A) C6H12O6
✅ B) C6H14O6
C) C12H22O11
D) C6H10O5
5. Hematoxylin requires what to effectively stain tissues?
✅ A) A mordant
B) A fixative
C) A clearing agent
D) A plasticizer
6. The process of enhancing hematoxylin’s staining capacity is
called:
✅ A) Ripening
B) Maturation
C) Polymerization
D) Precipitation
7. Natural ripening takes:
A) 1 week
B) 2 months
✅ C) 3–4 months
D) 10 days
8. Chemical ripening can be achieved using:
✅ A) Mercuric oxide
B) Sodium chloride
C) Acetic acid
D) Potassium iodide
9. Over-ripening occurs when:
✅ A) Excess oxidant is added
B) Not enough dye is present
C) Staining is too short
D) Sections are too thick
10. What is the function of a mordant in hematoxylin staining?
✅ A) Links dye to tissue
B) Fixes tissue
C) Dehydrates tissue
D) Destroys lipids
11. Common metals used as mordants include all EXCEPT:
A) Aluminium
B) Iron
C) Chromium
✅ D) Zinc
12. Traditionally, which salt is used as a mordant in hematoxylin
staining?
✅ A) Alum
B) NaCl
C) K2Cr2O7
D) Calcium chloride
13. In Harris’s hematoxylin, the ripening agent is:
✅ A) Mercuric oxide
B) Sodium nitrate
C) Sulphuric acid
D) Formalin
14. What is the role of glacial acetic acid in Harris’s hematoxylin?
✅ A) Enhances staining intensity
B) Decolorizes tissue
C) Fixes the dye
D) Clears background
15. Which is the correct sequence in preparing Harris’s
hematoxylin?
✅ A) Dissolve → boil → add HgO → cool → add acid
B) Boil → dissolve → filter → add acid
C) Cool → filter → add acid → boil
D) Filter → boil → dissolve → cool
16. What type of dye is eosin?
✅ A) Acidic
B) Basic
C) Neutral
D) Metallic
17. Eosin belongs to which dye group?
A) Azo group
✅ B) Xanthene group
C) Aniline group
D) Carbonyl group
18. Which of the following components are eosinophilic?
A) Nuclei
✅ B) Cytoplasm and connective tissue
C) Chromatin
D) Myelin sheath
19. Eosin Y is a derivative of:
✅ A) Fluorescein
B) Haematoxylin
C) Phenolphthalein
D) Pyronin
20. Eosin stains structures in shades ranging from:
A) Blue to green
✅ B) Orange to deep red
C) Yellow to purple
D) Pink to brown
21. Structures that stain well with eosin are called:
A) Basophilic
✅ B) Eosinophilic
C) Metachromatic
D) Chromogenic
22. What is the IARC classification of eosin?
A) Class 1
B) Class 2A
✅ C) Class 3
D) Class 4
23. Which of the following is NOT a commercial type of eosin?
A) Eosin Y
B) Eosin B
✅ C) Eosin Z
D) Eosin S
24. Which of the following can substitute eosin?
✅ A) Orange G phloxine
B) Sudan black
C) Toluidine blue
D) Cresyl violet
25. In eosin solution preparation, what enhances staining intensity?
✅ A) Potassium dichromate (K2Cr2O7)
B) Sodium bicarbonate
C) Ammonium sulfate
D) Acetic acid
26. What is the solvent used in preparing eosin solution?
✅ A) Distilled water
B) Xylene
C) Ethanol
D) Acetone
27. What is the typical eosin powder concentration?
✅ A) 1.0 g per 100 ml
B) 0.1 g per 10 ml
C) 5.0 g per 100 ml
D) 2.5 g per 50 ml
28. Hematoxylin is considered a(n):
A) Direct stain
✅ B) Indirect stain
C) Counterstain
D) Nuclear clearing agent
29. Alum is traditionally used because:
✅ A) It is inexpensive and easily available
B) It is the only effective mordant
C) It adds colour to eosin
D) It enhances solubility of glacial acid
30. In the staining procedure, when is glacial acetic acid added?
A) First step
✅ B) After cooling the mixture
C) Before boiling
D) During filtration
Would you like all the MCQs from the five presentations compiled into a
printable Word or PDF document with an answer key?
You said:
Connective Tissue Staining.pptx
Presentation
ChatGPT said:
Here are 30 multiple choice questions (MCQs) based on your uploaded
presentation "Connective Tissue Staining" by Dr. Muhammad Ilyas. Each
question has 4 options, with the correct answer marked.
1. Connective tissue primarily serves to:
✅ A) Connect and support body parts
B) Transport oxygen
C) Secrete enzymes
D) Control metabolism
2. Which of the following is not a fibrous component of connective
tissue?
A) Collagen
B) Elastin
C) Reticulin
✅ D) Actin
3. The most abundant type of collagen in connective tissue is:
A) Type II
B) Type III
✅ C) Type I
D) Type IV
4. Which staining technique is used for identifying connective tissue
fibers?
A) PAS
✅ B) Trichrome staining
C) Gram stain
D) H&E
5. Trichrome staining differentiates:
A) Neurons from glia
✅ B) Collagen from muscle fibers
C) Nuclei from cytoplasm
D) RBCs from WBCs
6. Trichrome staining includes all the following techniques EXCEPT:
A) Mallory’s stain
B) Masson’s stain
✅ C) PAS stain
D) Van Gieson stain
7. In H&E staining, both connective tissue and muscle appear:
✅ A) Eosinophilic
B) Basophilic
C) Metachromatic
D) Pale
8. In tumors, trichrome staining is especially useful because:
A) It stains nuclei
✅ B) It differentiates tissue origin
C) It measures mitosis
D) It reveals pathogens
9. Trichrome staining works based on:
✅ A) Molecular size of dyes and tissue permeability
B) Lipid solubility
C) Water content
D) Temperature of the dye
10. What factor affects permeability of tissue proteins in trichrome
staining?
✅ A) Lattice structure after fixation
B) Water content
C) Protein synthesis rate
D) Mitochondrial activity
11. Collagen shows what type of lattice structure?
A) Tight
B) Medium
✅ C) Loose and porous
D) Crystalline
12. Which component has the tightest lattice structure?
✅ A) Red blood cells
B) Collagen
C) Muscle fibers
D) Fat cells
13. In trichrome staining, permeability affects:
✅ A) Dye uptake based on molecular size
B) Tissue dehydration
C) Fixative diffusion
D) Nuclei integrity
14. PMA and PTA are used to:
✅ A) Clear collagen of small dye molecules
B) Fix tissues
C) Act as mordants
D) Acidify solutions
15. The belief that PMA/PTA are mordants is:
✅ A) Incorrect
B) Confirmed
C) Partially true
D) Not addressed
16. What replaces PMA in collagen fibers during staining?
✅ A) Aniline blue
B) Iron
C) Acid fuchsin
✅ D) Aniline blue/light green
17. Mallory’s trichrome is best used for:
✅ A) Collagen, reticulin, cartilage, bone, amyloid
B) Lipid droplets
C) DNA strands
D) Neuronal dendrites
18. What is the order of staining in Mallory’s method?
✅ A) Orange G → Acid fuchsin → PMA → Aniline blue
B) Aniline blue → PMA → Acid fuchsin → Orange G
C) PMA → Orange G → Acid fuchsin
D) Acid fuchsin → Aniline blue → Orange G
19. Orange G stains:
✅ A) RBCs
B) Nuclei
C) Cytoplasm
D) Collagen
20. Acid fuchsin in Mallory’s stain targets:
A) Collagen only
✅ B) Muscle and collagen
C) Nuclei only
D) Lipids
21. PMA/PTA displaces which dye from collagen?
✅ A) Acid fuchsin
B) Aniline blue
C) Orange G
D) Iron haematoxylin
22. Iron haematoxylin is preferred over Harris’s haematoxylin
because:
✅ A) It resists acidic solutions
B) It stains lipids
C) It is faster
D) It is fluorescent
23. In Mallory’s stain, aniline blue can be replaced by:
✅ A) Light green
B) Safranin
C) Eosin
D) Toluidine blue
24. Which trichrome technique uses methyl blue or light green?
✅ A) Masson’s trichrome
B) Van Gieson stain
C) PAS stain
D) H&E stain
25. What is used for nuclear staining in trichrome methods?
A) Harris’s haematoxylin
✅ B) Iron haematoxylin
C) Eosin
D) Orange G
26. Van Gieson stain solution contains:
A) Cresyl violet and ethanol
✅ B) Picric acid and acid fuchsin
C) Sudan black and methanol
D) Eosin and orange G
27. In Van Gieson’s method, which reagent stains nuclei?
✅ A) Iron haematoxylin
B) Methyl green
C) Toluidine blue
D) Basic fuchsin
28. For which tissue is Van Gieson stain especially useful?
A) Liver
B) Brain
✅ C) Connective tissue
D) Epithelium
29. What is a “lake” in the context of staining?
✅ A) Dye and mordant complex
B) Decolorized stain
C) Large pool of stain
D) Buffer solution
30. What does the PAP stain used in cytology share with trichrome
methods?
✅ A) Same principle of dye-mordant binding
B) Same fixatives
C) Identical dyes
D) Requires frozen sections
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