Unit III
Commercial Plant Breeding & Seed Industry
Contents
Quality Seed production of vegetable crops under an open and protected environment.
Alternative strategies for the development of the line and cultivars: haploid inducer,
tissue culture techniques and biotechnological tools.
Quality Seed production of vegetable crops under open
and protected environment
Quality seed production is a critical aspect of vegetable crop cultivation as it directly
influences crop performance, uniformity, and yield. The production of high-quality seeds
can be achieved under both open field conditions and protected environments, such as
greenhouses or polyhouses. Let's explore the key aspects of quality seed production for
vegetable crops in both settings:
1. Open Field Seed Production:
Selection of Parental Lines: High-quality seeds start with the selection of superior
parental lines based on desirable traits like disease resistance, yield potential, and
overall crop performance.
Isolation: To maintain genetic purity and prevent cross-pollination, adequate isolation
distance is maintained between different varieties or hybrids to avoid contamination.
Rogueing: Off-types or plants showing undesirable traits are removed promptly during
the growing season to prevent seed contamination.
Field Management: Proper agronomic practices, including irrigation, fertilization, and
pest management, are employed to ensure healthy plant growth and optimal seed
production.
Pollination Control: For cross-pollinated crops, proper pollination control methods like
bagging or caging are used to avoid unwanted cross-pollination.
Harvesting and Drying: Seeds are harvested at the correct maturity stage and carefully
dried to the optimum moisture level to maintain seed quality.
2. Protected Environment (Greenhouses or Polyhouses) Seed Production:
Climate Control: In protected environments, temperature, humidity, and light conditions
are carefully controlled to provide an optimal environment for seed production.
Pest and Disease Management: Strict biosecurity measures are followed to prevent the
entry of pests and diseases into the protected environment. Integrated pest
management (IPM) practices are employed to minimize the use of chemical pesticides.
Pollination: For crops requiring pollination, such as tomatoes or cucurbits, controlled
pollination methods are used to ensure genetic purity.
Support Structures: Trellising or staking is often employed to support the growth of
plants and prevent lodging.
Enhanced Yield Potential: Under protected environments, crops can be grown
throughout the year, allowing for multiple seed production cycles and higher overall
seed yield.
Quality Assurance and Testing: Seeds produced in protected environments undergo
rigorous quality testing to ensure genetic purity and germination viability.
Commercial Plant Breeding & Seed Industry 2
Benefits of Quality Seed Production:
1. Uniformity: High-quality seeds lead to uniformity in crop growth and
performance, making crop management and harvesting more efficient.
2. Resistance: Quality seeds from resistant parent lines contribute to improved
disease and pest resistance in the resulting crops.
3. Yield Potential: Well-produced seeds exhibit higher yield potential, ultimately
benefiting farmers with increased productivity.
4. Market Demand: High-quality seeds are in demand in the market, attracting more
buyers and enhancing the reputation of seed producers.
Quality seed production of vegetable crops is essential for achieving successful and
profitable vegetable cultivation. Whether in open fields or protected environments,
attention to detail in breeding, isolation, pollination control, and field management
ensures the production of genetically pure and high-performing seeds.
Alternative strategies for the development of the line and
cultivars
Traditional plant breeding methods have been highly successful in developing improved
lines and cultivars. However, in recent years, alternative strategies that incorporate
modern biotechnological tools have emerged, enhancing the efficiency and precision of
crop improvement. Three notable alternative strategies are haploid inducer technology,
tissue culture techniques, and biotechnological tools. Let's explore each strategy in
detail:
1. Haploid Inducer Technology:
Concept: Haploid inducer technology involves the creation of haploid plants (with half
the normal chromosome number) through the process of haploid induction.
Process:
● A haploid inducer line is developed, possessing specific genes that promote
haploid formation during pollination.
● The haploid inducer is crossed with the target crop, and the resulting progeny
inherit the haploid-inducing genes.
Commercial Plant Breeding & Seed Industry 3
● The inducer lines are usually male sterile, preventing self-pollination and
promoting hybrid seed production.
Benefits:
● Rapid Development of Pure Lines: Haploid inducer technology allows the rapid
development of pure homozygous lines, significantly reducing the breeding time.
● Hybrid Seed Production: Male sterility in the inducer lines facilitates hybrid seed
production, ensuring genetic purity and higher seed yields.
● Precision Breeding: This technology enables precise selection and transfer of
desired genes, leading to the development of superior cultivars.
2. Tissue Culture Techniques:
Concept: Tissue culture is the aseptic culture of plant cells, tissues, or organs in nutrient
media, leading to the formation of whole plants.
Process:
● Plant material, such as seeds, embryos, or explants (small pieces of plant tissue),
is sterilized to prevent contamination.
● The explants are cultured in a nutrient medium containing essential nutrients and
growth regulators.
● Under controlled conditions, the explants differentiate and grow into new
plantlets.
● The regenerated plantlets are transferred to soil to grow into mature plants.
Benefits:
● Clonal Propagation: Tissue culture allows rapid clonal propagation of plants with
desirable traits, maintaining genetic uniformity.
● Disease Elimination: Infected plant material can be effectively treated, and
disease-free plants can be produced.
● Genetic Transformation: Tissue culture enables the introduction of specific
genes into plants, leading to genetically modified crops with desired traits.
3. Biotechnological Tools:
Commercial Plant Breeding & Seed Industry 4
Concept: Biotechnological tools refer to advanced techniques used for genetic
manipulation, such as genetic engineering and genome editing.
Process:
● Genetic Engineering: Specific genes are inserted or modified to introduce desired
traits into the plant's genome.
● Genome Editing: Advanced techniques like CRISPR-Cas9 are used to precisely
edit specific genes, enabling targeted modifications.
Benefits:
● Trait Incorporation: Biotechnological tools allow the incorporation of beneficial
traits, such as disease resistance, herbicide tolerance, and improved nutritional
content.
● Precision and Efficiency: These tools offer precise and efficient gene editing,
accelerating the development of improved cultivars.
● Trait Stacking: Multiple desired traits can be stacked in a single plant, creating
cultivars with a combination of beneficial characteristics.
By incorporating these modern tools into the traditional plant breeding process,
breeders can efficiently develop superior cultivars with improved traits, contributing to
global food security and sustainable agriculture.
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Agricorn - Commercial Plant Breeding & Seed Industry
Commercial Plant Breeding & Seed Industry 5