Check up

 General check up
 CBC
 ESR
 FBS & 2hrpp
 GPT & GOT
 Creat & urea &uric acid
 Lipid profile
 TSH &FREE T4
 Urine & stool

CHEMISTRY UNIT
 Lipid profile
● Total cholesterol

● Triglyceride

● HDL

● LDL

● VLDL

 Liver function
● Billirubin “Total & Direct”

● GPT (Alt)

● GOT(Ast)

● GGT

● Alkaline Phosphatase

● Albumin
● Total protein

● PT,Pc,INR

 Kidney or Renal function

● Creat

● Urea

● Uric acid

● K+

● Na+

● Mg+2

● Mn+2

● Phosphorus

● Calcium “Total &ionized”.

● Chloride

● Urine analysis

● microalbuminurea

● *Creatinin Clearance

● *E. GFR

● *Cystatin C

 Cardiac profile
● LDH ”lactate dehydrogenase”

● Ck total or CPk

● Ck MB

● Troponin (I &T)

● Myoglobin
● GOT

● D. Dimer

● Homocystiene
 Diabities profile
● FBS

● PP

● Hb A1C “Glycated Haemoglobin”

● Fructoseamine

● RBS

● Insulin “Fasting & PP”

● C. Peptide “Fasting &PP”

● Glucose curve

● Pregnant “Fasting, 1hr,2hr,3hr”

● Non pregnant & Male ”Fasting, 1/2hr,1hr,1 ½ hr, 2hr”

● Homa IR

● Anti Insulin Ab

● Anti Islet cell Ab

● Anti GAD Ab

VIROLOGY UNIT
Hepatitis A virus
● HAV “IGG, IGM”
Hepatitis C virus
● HCV Ab
● Riba test

● HCV by pcr “Qualitative, Quantitative”

● HCV genotyping

Hepatitis B virus
● HBs Ag

● Hbs Ab

● HBe Ag

● HBe Ab

● HBc IgM

● HBc Total
● HBV DNA by PCR “qualitative, quantitative”
Hepatities D ‘’DELTA VIRUS
 HDV AG
 HDV ‘’TOTAL &IGM’’
Hepatities E VIRUS
 HEV IGG
 HEV IGM
 HEV PCR
HIV

● HIV Ab

● Western Blot

● HIV PCR “qualitative, quantitative”

Measles & Mumps
  IGG &IGM
 CULTURE
 PCR
*Cytomegalovirus
 CMV “IGG &IGM’
 CMV PCR
*Epstein-Barr virus
 EBV “IGG &IGM” VCA
*Rubella
 Rubella “IGG &IGM”
*Herpes Simplex
 HSV I “IGG &IGM”
 HSV II”IGG &IGM”
*Human Papilloma Virus
 HPV AG
 HPV “IGG &IGM”

*ROTA VIRUS in stool

Sexual Transmitted Disease

STD
VIRUS
 HIV
 HBV
 HSV’’I&II’’
 HPV
 Syphilis
 TPHA
 VDRL
 RPR

Chlamydia
 Ag & Ab

“ Abortion”

-TORCH
● Toxoplasmosis “IGG, IGM”

● Rubella “IGG, IGM”

● CMV “IGG, IGM”

● Herpes

● HSV I “IGG, IGM”

● HSV II “IGG, IGM”

-Anti Phospholipid

● Lupus Anticoagulant

● Anticardiolipin “IgG, IgM”

● Nk cells

● Protien C

● Protien S

● Anti thrombin III

● Factor V liden mutation

● Thrmbophilia gene

Tumar Marker
*Leukemia
 “CBC, TlC “WBC”
 ABL-BCR
 Philadelphia Chromosome
*Breast
 CA 15.3
*OVARY
 CA 72.4
 CA 125
*Uterus
 PAP Smear
 SCC
 TPA
 TPS
*Colon
 CEA
 CA 19.9
  Occult blood in Stool
 Schebo Test “M2_PK” STOOL
 Calprotectin
*Pancreas
 Amylase
 Lipase
 CA 19.9
*Stomach
 Gastrin
*Liver
 AFP
 PIVKA II
 Ferritin
*LUNG
 NSE
 LTA
*Kidney
 Renin
 Erythropoietin
*THYROID GLAND
 Thyroglobulin
 Calcitonin
*Adrenal Gland ‘’ Pheochromocytoma ’’
 “ Catecholamine, Dopamine”
 “Epinephrine, Nor Epinephrine”
 VMA
*Pituitary Gland
 ACTH
 Prolactin
 GH
*Prostate
 PSA ‘’TOTAL,FREE’’
*Bladder
 CA50
 TPA
 TPS
 UBC
*Testis
 BHCG
 AFP

HORMONES UNIT
THYROID GLAND
● TSH
● T3” Total ,Free ”
● T4”Total ,Free ”
Hypo Thyrodism Hyper Thyrodism
TSH “High” TSH “low”

T3&T4 “low” T3&T4 “High”

Anti Thyroid Ab “Auto immune Disease”

● Anti Thyroglobulin AB
● Anti Peroxidase AB ’’Anti Microsomal AB ‘’
Para Thyroid Gland
● Para Thyroid Hormone “PTH” “EDTA PLASMA”
● Calcium Total TO IoniZed Calcium

Adrenal GLAND
* CORTEX
 Aldosterone
 Cortisol “ 9 -10am, 9 - 10pm”
 17 OH Cortisol
 DHEAS
*MEDULLA

● Adrenaline ’’ Epinephrine ’’ & noradrenaline ’’ norepinephrine ’’

● VMA

PITUITARY GLAND

● TSH
● ACTH(9-10 AM /9-10 PM)
● PROLACTIN
● FSH
● LH
● GH (basal& Stimulation ”Activity, Clonidine,Dopa,Insulin ”)
● ADH
● Oxytocin
GONODAL HORMONE
MALE (Infertility)

● TESTOSTERON “Total ,Free”

● FSH
● LH
● PROLACTIN
● Semen Analysis
 ● Anti Sperm Ab
FEMALE (Infertility)

● FSH
● LH
● PROLACTIN
● PROGESTERON
● ESTRADIOL “E2”
● TESTOSTERON “Total &Free”
● AMH “Anti Mullerian Hormone”
● Inhibin B
● Anti Sperm Ab

Down Syndrome
*Double Marker (First Trimester 8-13 weeks)
 BHCG
 PAPP (Placenta Associated Plasma Protein)
*Triple Marker (Secand Trimester 14-22 weeks)
 BHCG
 ESTRIOL ‘’E3’’
 AFP
*Neobona "NIPT"
 Trisomies 21, 18, and 13
 Fetal sex

HEMATOLOGY UNIT
*Comlete Blood Picture
 HB
 RBCs
 Platelet
 WBCs
Parameter Increase Decrease
RBCs  Polycythemi  Anemia
a  Hemolysis
 Dehydration  Chronic illness
 Iron & Vit B 12
Platelets Thrombocytosis Thrombocytopenia
 Acute  Leukemia
bleeding  Anemia
 Cancer  HCV ,HIV
 Infection  Chemotherapy
 Iron  Alcohol
deficiency
 Haemolytic
anemia
 Remove
spleen
 Surgery

WBCs Leucocytosis Leukopenia

 leukemia  Chronic infection
 Inflammatio  Antibiotics
n  Typhoid
 Pregnancy
 New born
 Acute
bacterial
infection
Neutrophil  Acute  Anemia
s infection  Bacterial infection
 Rheumatoid  Chemotherapy
artharitis  Radiotherapy
 pregnancy
lymphocyt  Inflammatio  Chemotherapy
e n  HIV Infection
 Chronic
infection
 leukemia
Monocyte  Chronic  Blood infection
 inflammator  Chemotherapy
y disease  Bone marrow disorder
 Tuberculosis
 Viral’measle
s,mumps
eosinophil  Allergic  Acute allergic
s reaction
 Parasitic
infection
basophils  Blood cancer

*Coagulation Profiles
 BT
 CT
 PT
 PTT
 Platelet Function(Aggregation
 Factor V
 Factor VIII
 Factor IX
 Protein C
 Protein S
 Anti Thrombin III
 Thrombophelia Gene
 FDPs

Factor of Coagulation
Factor Name
I Fibrinogen
II Prothrombin
III Tissue factor or thromboplastin
IV Calcium ++
V Proaccelerin (Labile factor)
VI Unassigned
VII Proconvertin (Stable factor
VIII Antihaemophilic factor A,
Antihaemophilic globulin
IX Antihaemophilic factor B,
Plasma thromboplastin
component,
Christmas factor
X Stuart- Prower factor
XI Plasma thromboplastin
antecedent,
Haemophilia C,
Rosenthal syndrome
XII Hageman factor
XIII Fibrin stabilising factor,
 ANEMIA
Deficiency of haemoglobin

NORMOCYTIC NORMOCHROMIC ANEMIA

 Chronic Disease
 Drug Therapy

*HEMOLYTIC ANEMIA
1-FAVISM
● G6PD “Qualitative ,Quantitative”
2-THALLASSEMIA
● Hb electrophoresis “Hb F”
3-SICKLE CELL ANAEMIA
● Hb electrophoresis “Hb S”
4-SPHEROCYTOSIS
● Osmotic Fragility
5-AUTO IMMUNE
● Coombs TEST “Direct & indirect”

MICROCYTIC ANEMIA
*IRON PROFILE
● IRON
● FERRITIN
● TIBC
● Vit C

MACROCYTIC ANEMIA
● FOLIC ACID
● VIT B 12
PROFILES

FEVER
 WIDAL
 BRUCELLA
 MONOSPOT “POWEL DISEASE”
 CMV “IGG ,IGM”
 EBV “IGG ,IGM”
 Rubella ,Measles ,Mumps
 HIV
 BLOOD” CULTURE &SENSITIVITY”
 Urine “C&S”
 FMF ”Familial Mediterranean Fever” ‘’AMYLOID A’’
 Q fever (Dengue fever)→ Coxiella IGM and IGG, RT PCR.
 MALARIA “THIN ,THICK FILM” EDTA
 Tuberculosis
 ANA
 CBC
 ESR
 CRP
 LDH
 LIVER Enzymes

Rheumatoid Arthritis
 RF “qualitative & quantitative”
 CRP” qualitative & quantitative”
 Rose Waller
 Uric acid
 ESR
 Anti CCP
 Anti MCV
 HLA B27

Rheumatic Fever
 ASOT
 CRP
 ESR

Osteoporosis
 Calcium “Total & ionized”
 Phosphorus
 Alkaline phosphatase
 Osteocalcin
 Vit. D
 PTH
 DPD

Hypertension

 Renin
 VMA
 Aldosterone
 Catecholamine
 Dopamine
 Adrenaline & noradrenaline

Tuberclosis
 Tuberculin test
 AFB
 TB examination “’one sample ‘’
 Z/n stain
 TB C&S
 Bactec Culture
 Quantiferon TB Gold “IGRA”
  TB by Pcr
 ADA ‘’body fluid’’

ALLERGY
 IGE TOTAL
 IGE Specific ‘RAST’ ‘’Food & Inhalent’’
 Skin Test

Culture
*BACTERIA
 Urine ex. C&S
 Stool ex. C&S
 Semen ex.C&S
 Prostatic’’ Massage ‘’Discharge ex. C&S
 Urethral Discharge ex. C&S
 Vaginal Discharge ex. C&S
 Cervical Discharge ex. C&S
 Blood C&S
 Ear Discharge ex. C&S
 Conjunctival Discharge ex. C&S
 Nasal Discharge ex. C&S
 Throat swab ex. C&S
 Pus .C&S
 Sputum ex. C&S
 Nipple Discharge ex. C&S

*FUNGI
 Fungus examination
 Fungus C&S

Helicobacter
 H. Pylori AG in Stool
 H. Pylori AB
 H. Pylori IGG ,IGA
 Urea Breath Test

Pre-Operative
 CBC
 PT, PTT
 RBS
 GPT, GOT
 CREAT, UREA
 HBs Ag ,HCV Ab
 KIDS’’ASOT, CRP, ESR’’

Premarital
 CBC
 RBS
 ABO-Rh
 HBs AG
 HIV
 VDRL

*Male
 Semen analysis
 *Female
 FSH
 LH
 Prolactin
 E2
 Progesterone
 AMH
 TORCH ‘IGG , IGM’
*Related
 Hb-electrophoresis
 Karyotyping
 Aminogram

Hirsutism
 FSH
 LH
 PROLACTIN
 DHEA-S
 17 –OH Progesteron
 Testosterone ‘Total &Free’
 DHT
 Androstendione
 Cortisol ‘’AM ,PM’’

HAIR LOSS
*NUTRITION
 CBC
  IRON
 FERRITIN
 TIBC
 ZINC
 Folic Acid
 VIT. B12
 VIT. D ’’25 OH’’
 Copper
*HORMONES
 Testosterone ‘’Total &Free’’
 DHEA-S
 DHT
 FSH
 LH
 PROLACTIN

Disputed Paternity
 DNA Sequencer
 HLA Type I
 HLA Type II
 ABO &Rh
 Rh- Phenotype

Trasnplantation Profiles
 Cross match
 HLA Type I
 HLA Type II
 ABO &Rh
 Rh- Phenotype

Drug Monitoring
 Cyclosporine ‘’Sandimmune’’ C 0 & C 2
 Tacrolimus ‘’FK 506’’ ’’Paragraph’’
 Sirolimus
 Lithium
 Digoxin
 Methotrexate
 Theophylline

Antiepileptics

 Depakine 5
 Epanutin 6
 Tegretol 8

TOXOCOLOGY PANEL
 Amphetamine ‫اﻟﻤﻨﺒﻬﺎت‬
 Barbiturates‫اﻟﻤﻨﻮﻣﺎت‬
 Benzodiazepines‫اﺑﻮﺻﻠﻴﺐ ه‬
 Cocaine‫ﻛﻮﻛﺎﻳﻦ‬
 Morphine ‫ﻣﻮرﻓﻴﻦ‬
 Cannabinoids ‫ﺣﺸﻴﺶ وﺑﺎﻧﺠﻮ‬
 Tramadol ‫ﺗﺮاﻣﺎدول‬
 Alcohol ‫ﻛﺤﻮل‬
 Pathology unit

*Cytology
1-Urine
2-Nipple Discharge
3-Body Fluid ( Examination , C&S , Chemistry)
 CSF
 Synovial Fluid
 Pericardial Fluid
 Pleural Fluid
 Peritoneal Fluid ( Ascitic )

*Biobsy
 Small
 Medium
 Large

*FNAC
Fine Needle Aspiration Cytology
*Immunohistochemistry
*Immunophenotyping
*Slides for Consultation
 IMMUNOLOGY UNIT
Antibodies (Immunoglobulins
“IGM,IGA,IGG,IGE,IGD”
Auto Immune Disease
*Systemic Lupus Erythematosus
 ANA
 ADNA
 Anti Smith
 LE Cell
 Anti SSA (RO)
 Anti SSB (LA)
 C3
 C4

*Liver
 LKM (Liver Kidney Microsomal)
 AMA (Anti Mitochondria Antibody)
 ASMA (Anti Smooth Muscle Antibody)

*Kidney
 AGBM (Anti Glomerular Basement Membrane)

*Thyroid
 Anti Thyroglobulin AB
  Anti Peroxidase AB OR Anti Microsomal AB

*Colon
 ASCA
 ANCA

*Pancreas
 Anti Islet cell AB
 Anti Insulin AB
 Anti GAD AB

*Sperm
 Anti Sperm AB

*Derma
 Anti SCL 70 AB

*Celiac Disease
 ATTG ‘’Anti Tissue Transglutaminase IgA & IgG
 AGA ’’Anti Glidin Ab’’

*Gastric
 APA (Anti Parietal Ab )

*Muscle
 JO-1
Collected 24hr urine
With 10 ml HCL With OUT
  VMA  Creatinin Clearance
 5 HIAA  Albumin
 Hydroxy Prolen  Protein
 Inorganic Phosphorus  Na
 Mercury  Uric acid
 Lead  Glucose
 Oxalate
 Copper
 Calcium
 Catecholamine
 k

Frozen sample
serum  Insulin
 c-peptide
 GH
EDTA PLASMA  ACTH
 PTH
 Ammonia
CITRATE PLASMA  PTT
 Lupus
Anticoagulant
 Protein C
 Protein S
 Anti Thrombin III
 D-dimer